valentina hällefors physics sbcc ucsb detecon of ... hallefor… · of forensic significance...
TRANSCRIPT
Detec%onofSubstancesofForensicSignificanceUsingMicrofluidicsand
SERSFunding
Rustin Mirsafavi BMSE
Carl Meinhart Mechanical Engineering
Valentina Hällefors Physics SBCC UCSB
Real world applications Homeland security, medicine, drug trafficking
Our Research
The devices Inexpensive and reproducible
Real world applications Homeland security, medicine, drug trafficking
Our Research
The devices Inexpensive and reproducible
Microfluidics Control small amounts of fluid in a predictable way
Surface-enhanced Raman spectroscopy (SERS) Analyze composition, small sample volume
Real world applications Homeland security, medicine, drug trafficking
Our Research
This Summer:
• Relate Raman spectra to concentration
Knownconcentra%on
This Summer:
• Relate Raman spectra to concentration
Knownconcentra%on
• Get Raman spectra for different concentrations
This Summer
• Relate Raman spectra to concentration
Knownconcentra%on
• Get Raman spectra for different concentrations
• Build devices
Data Collection
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2
3
Sample preparation
Data Collection
1
2
3
Sample preparation
• 0.1-100 µM sample • Flow salt (LiCl) • 20 nm AgNPs
Data Collection
1
2
3
Microscope & fluidic processes
Data Collection
• Low velocity creates separated flow
• Mixture of fluids purely driven by diffusion
• Salt induces aggregation of silver necessary for strong signal
Fentanyl
Data Collection
1
2
3 Laser and SERS
Data Collection
5 min
• Map channel section with 633 nm laser
• Run device 4-5 times
• Stop after 10-12 minutes 10 min
Data – Noscapine/Papaverine
(100µM)
(100µM)
Data – References
Heroin
6-Monoacetylmorphine
Progress to date
Lab work Microfluidic devices, Raman microscope
Papaverine and noscapine Gathered data for 100uM
Reference spectra Heroin, morphine, 6-MAM
! Test broader range of concentrations
! Obtain data for other substances
! Spectra-concentration relationship
Future work
Acknowledgments
Thank You!