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Personal Purification SystemUser’s Guide

PLC 2020 Personal Purification System User’s Guide TOC-1

Safety

Introduction

Description ........................................................................................ 2-2

Standard Equipment ...................................................................... 2-3Documentation ....................................................................... 2-4Accessories ................................................................................ 2-4Optional Accessories ............................................................. 2-6

Training ............................................................................................... 2-7

Customer Service ............................................................................. 2-7

Technical Specifications ................................................................ 2-7

Operating Environment ..............................................................2-12Temperature ...........................................................................2-12Contaminants .........................................................................2-12Ventilation ...............................................................................2-12

Setup/Operation

Shipping Screw Removal .............................................................. 3-2Tools Required ......................................................................... 3-2

Pump Head Installation ................................................................. 3-3

Flow Cell Installation ...................................................................... 3-4

Injection Port Installation ............................................................. 3-5

Tubing Clip Installation ................................................................. 3-5

Rack Installation ............................................................................... 3-5

Table of Contents

TOC-2 PLC 2020 Personal Purification System User’s Guide

Tabl

e of C

onte

nts Rear Panel Connections ................................................................ 3-6

Analog Output Channels ..................................................... 3-7USB Ports .................................................................................... 3-7Ethernet and GSIOC Ports .................................................... 3-8Input and Output Ports ........................................................ 3-8Power Cord Connection ...................................................... 3-9

Accessory Installation ..................................................................3-10Column Holder Bracket and Waste Bottle Bracket Installation ...............................................................................3-10

Plumbing Connections ................................................................3-11Plumbing Connections (A–H) ...........................................3-12Plumbing Connections (I–K and O) ................................3-13Plumbing Connections (L–P) ............................................3-14Plumbing Connections for the FC Valve (Optional) ..3-15

Startup ...............................................................................................3-17

Software Operation ......................................................................3-17

Priming the PLC 2020 ...................................................................3-17

Conditioning the Pump Heads .................................................3-18

Shut Down the PLC 2020 ............................................................3-18

Cleaning

Helpful Hints ...................................................................................... 4-2

System ................................................................................................. 4-2

Touchscreen ...................................................................................... 4-3Shut Down the PLC 2020 ..................................................... 4-3Clean the Touchscreen .......................................................... 4-3

Fluid Path ............................................................................................ 4-4Cleaning Methods .................................................................. 4-4

Cleaning the Inside of the Flow Cell ......................................... 4-6Solvent Cleaning ..................................................................... 4-6Acid Cleaning ........................................................................... 4-7

Unclogging the Flow Cell ............................................................. 4-8

PLC 2020 Personal Purification System User’s Guide TOC-3

Table of Contents

Replacing Parts

Tubing .................................................................................................. 5-2Stainless Steel Tubing and Fittings ................................... 5-2Other Tubing and Fittings .................................................... 5-2

Detector .............................................................................................. 5-4Flow Cell Assembly ................................................................. 5-4Tubing and/or the Flow Cell................................................ 5-6Visible Lamp ............................................................................5-10UV Lamp ...................................................................................5-12

Pumping System ............................................................................5-15Pump Heads ............................................................................5-16Check Valves ...........................................................................5-17Piston Seal ...............................................................................5-19Piston Assembly ....................................................................5-22Inlet Filter .................................................................................5-24In-Line Filter ............................................................................5-24

Fraction Collector ..........................................................................5-25Fraction Collection Probe ..................................................5-25Fraction Collector Valve Assembly ..................................5-25

Injection Valve ................................................................................5-28Injection Valve ........................................................................5-28Injection Port ..........................................................................5-29Injection Port Liner ...............................................................5-29Injection Valve Rotor ............................................................5-30

Fuse .....................................................................................................5-32

Additional Replacement Parts ..................................................5-32

TOC-4 PLC 2020 Personal Purification System User’s Guide

Tabl

e of C

onte

nts Troubleshooting

Error Codes and Messages ........................................................... 6-2Obtain Error Code and Message........................................ 6-2PLC 2020 Error Codes ............................................................ 6-2Detector Error Codes ............................................................. 6-3

Troubleshooting .............................................................................. 6-4Detector Troubleshooting ................................................... 6-5Hydraulic (Pump) Problems ................................................ 6-8Dampener .................................................................................. 6-9Injection Valve ........................................................................6-11

Repair and Return Policies..........................................................6-12Before Calling Us ...................................................................6-12Warranty Repair .....................................................................6-12Non-Warranty Repair ...........................................................6-12Return Procedure ..................................................................6-13Unit End-of-Life .....................................................................6-13

Reference

Compressibility ................................................................................. 7-2

Solvent Miscibility Table ................................................................ 7-4

Mobile Phase Tips ............................................................................ 7-5Check UV and Visible Absorbance of Solvents ............. 7-5

PLC 2020 Personal Purification System User’s Guide 1-1

Chapter 1

Read this section before installing and operating the PLC 2020 Personal Purification System.

The PLC 2020 is intended to be used in a laboratory environment by trained technical personnel.

For safe and correct use of this system, it is recommended that both operating and service personnel follow the instructions contained in this guide when installing, cleaning, and maintaining the system.

The following safety precautions must be observed during all phases of operation, service, and repair of the system. Failure to comply with these precautions or with specific warnings elsewhere in this user’s guide violates safety standards of design, manufacture, and intended use of the system. Gilson assumes no liability for the customer’s failure to comply with these requirements.

The PLC 2020 Personal Purification System has been certified to UL, CSA, and CE Safety standards and has been tested to the requirements of CAN/CSA-C22.2 No. 61010-1, second edition, including Amendment 1, or a later version of the same standard incorporating the same level of testing requirements.

Safety

1-2 PLC 2020 Personal Purification System User’s Guide

Safe

ty The following electronic and hazard symbols may appear on the system:

Symbol Explanation

~ Alternating current

Courant alternatif

Wechselstrom

Direct current

Courant continu

Gleichstrom

Protective conductor terminal

Borne de terre de protection

Schutzleiteranschluss

| Electrical power ON

Sous tension

Netzschalter ein

O Electrical power OFF

Hors tension

Netzschalter aus

Caution

Attention

Vorsicht

Caution, risk of electric shock

Attention, risque de choc électrique

Vorsicht, Elektroschockgefahr

Caution, hot surface

Attention, surface chaude

Vorsicht, heiße Oberfläche

Caution, ultraviolet light

Attention, rayonnement ultraviolet

Vorsicht, Ultraviolettes Licht

Fuse

Fusible

Sicherung


Safety

The following safety notices may appear in this document:

WARNING indicates a potentially hazardous situation which, if not avoided, may result in serious injury

CAUTION indicates a potentially hazardous situation which, if not avoided, may result in minor or moderate injury

NOTICE indicates a potentially hazardous situation which, if not avoided, may result in equipment damage

Ultraviolet (UV) Radiation

The deuterium lamp emits UV radiation that is harmful to the eyes. Always turn off the system power before removing the lamp cover.

Never look directly into the light from the deuterium lamp. Always use protective glasses with a UV filter, if you have to open the lamp housing while the deuterium lamp is on.

Voltage

Access to the rear panel is necessary. The system must be detached from all voltage sources before service, repair, or exchange of parts.

For normal operation, the system is to be grounded through the AC line cord provided. Failure to do so can result in a potential shock hazard that could result in serious personal injury.

Use only fuses with the rated current and of the specified type as listed on the rear panel of the system.

The system must only be operated with the voltage specified on the rear panel label of the system using a grounded AC line cord.

Heat

The deuterium lamp can reach temperatures of 176°F (80°C) in normal operation. Turn off the system power and then allow 30 minutes for the lamp to cool before servicing/replacing.


Safe

ty Probes

Because the fraction collection probe may contain a dangerous substance, do not interfere in the work area of the system until the system has completed its procedures.

Solvents

Observe safe laboratory practices when handling solvents. If dangerous liquids are used, adequate protection such as proper ventilation, safety glasses, etc., should be used.

Refer to the Material Safety Data Sheets for all solvents before use.

Replacement Parts

Be sure to use only replacement parts mentioned in Chapter 5, Replacing Parts. Do not repair or change parts which are not listed in this user’s guide. If it is necessary to change parts not listed, please contact your Gilson-authorized representative.


Chapter 2

Contents

Description ................................................................................... 2-2

Standard Equipment ................................................................. 2-3

Training .......................................................................................... 2-7

Customer Service ....................................................................... 2-7

Technical Specifications ........................................................... 2-7

Operating Environment .........................................................2-12

Introduction


Intro

duct

ion Description

The PLC 2020 Personal Purification System is a compact and independent platform capable of performing manual injection purification runs from 1–100 mL/min with pressures up to 4060 psi (depending on pump head size). The integrated pumping system, variable–dual wavelength UV/VIS detection, fraction collection and touchscreen controller allows the system to be completely self-contained and operated without the need for any peripheral equipment.


Introduction

Standard Equipment

After the PLC 2020 and accessories have been unpacked, you should have these items:

PLC 2020 Personal Purification System (part number 21130000)

PLC 2020 Accessory Package (part number 21037020) which includes:

Part Number Description21037010 PLMB PKG,INJ VLV PLC includes:

49400007 PORT,MNL INJ VLV .75 20GA SST49953059 TUBING, .030" X 5FT, PEEK4903180411 VALCO PEEK NUT 1/16, 1 EA (Qty 2)4903180511 VALCO PEEK FERRULE 1/16, 1 EA (Qty 2)499331519 TUBING, .030" ID X 5FT, SS490318011 VALCO NUT SS,ZN1 (Qty 2)490318021 VALCO FERRULE SS,ZF1S6 (Qty 2)49041001 FITTING,FINGERTIGHT,10-32,KEL-F(F-100N) (Qty 2)495033 TUBING, .5MM ID X 1.5MM OD,TFE 10 FT/PK

21037011 PLMB PKG,PMP PLC includes:21037000 TBG ASSY,25/50 SC PMP “A” TO DMPNR PLC21037001 TBG ASSY,25/50 SC DMPNR TO PPMM PLC21037015 TBG ASSY,100 SC PMP “A” TO DMPNR PLC21037016 TBG ASSY,100 SC DMPNR TO PPMM PLC21037002 TBG ASSY,PMP “B” TO PPMM PLC21037008 TBG ASSY,PPMM TO WASTE PLC47014386301 TUBE,VITON .313IDx.438OD 10FT21037021 SYRINGE, PRIME PLC36610101 WRENCH, 5/16 - 1/4

21037013 PLMB PKG,SLVT SEL VLV PLC includes:21037005 TBG ASSY,SLVT INLET (Qty 2)4957232 INLET FILTER,20UM 5/16-24 FOR 3/16 TBG (Qty 2)21037003 TBG ASSY,SLVT VLV TO PMP “B”49010000 FTG,5/16-24 TO 1/4-28 M PEEK ADPTR (Qty 2)49010001 FTG,5/16-24 F TO M10x1.5 PEEK ADPTR (Qty 2)54096003 CLA,CBL1.18 DIA ADH BACKED RICHCO SHR-C3

(Qty 3)54118051 SPIRAL WRAP,1/2" BLACK21037022 EXTND TOOL,5/16-24 DIBA FTG

21031063 BRKT, COL HLDR PLC3645292 CLAMP, SC PUMP HEAD (Qty 2)400956161 M6 X 16MM KNURLED T KNOB SCREW (Qty 2)


Intro

duct

ion Part Number Description

7080318107 POWER CORD, 18/3, 110V7080316106 POWER CORD, DETACHABLE, 220V4320302 ALLEN WRENCH, 3MM4311562 ALLEN WRENCH, 5/32"

Documentation

The following documents are included with the PLC 2020:

• Setup Guide

• User’s Guide

• Software Quick Reference Guide

• Installation Qualification Procedures

• Unpacking Instructions

• Declaration of Conformity

• Software Validation Certificate

• Quality Control (QC) Checklist

• Software License Agreement

Accessories

Based upon your configuration, you also ordered and received additional accessories, including the pump heads, sample loops, flow cell assemblies, racks, etc.

Pump Heads

Part Number Description360522 PUMP HEAD, 25SC, 25 ML/MIN, 4060 PSI360524 PUMP HEAD, 50SC, 50 ML/MIN 2030 PSI360530 PUMP HEAD,100SC, .50-100ML/MIN, 1015 PSI

Sample Loops

Part Number Description49440007 SAMPLE LOOP, 20UL, SS, VALCO, 1/16"49440008 SAMPLE LOOP, 50UL, SS, VALCO, 1/16"49440009 SAMPLE LOOP, 100UL, SS, VALCO, 1/16"


Introduction

Part Number Description494400002 SAMPLE LOOP, 250UL, SS, VALCO, 1/16"494400005 SAMPLE LOOP, 500UL, SS, VALCO, 1/16"49440001 SAMPLE LOOP, 1ML, SS, VALCO, 1/16"49440002 SAMPLE LOOP, 2ML, SS, VALCO, 1/16"49440005 SAMPLE LOOP, 5ML, SS, VALCO, 1/16"

Flow Cell Assemblies

Part Number Description100441 CELL BLOCK ASSY, 0.2MM, QUARTZ100443 CELL BLOCK ASSY, 0.05MM, QUARTZ1004395 FLOW CELL, PREP HIGH PSI, 2 UL, .5MM

Syringe and Needles (for Manual Injection)

Part Number Description54350100 SYRINGE,10CC LUER LOK PLASTIC 100PK54350101 SYRINGE,1CC LUER LOK PLASTIC 100PK54350302 NDL,20GA 1" LUER LOK W/TIP GUARD 100PK54350303 NDL,20GA 1" LUER LOK BLUNT TIP REUSE 3PK

Racks

Part Number Description260440079 RACK,CODE 332 44 18x150MM TUBES260440036 RACK,CODE 333 14 20mL SCINT VIALS260440081 RACK,CODE 334 14 40ML SCINT VIALS260440083 RACK,CODE 335 48 15x45MM WISP VIALS260440025 RACK,CODE 343 80 13x100MM TUBES260440041 RACK,CODE 345 44 16x150mm TUBES260440049 RACK,CODE 346 44 16x100MM TUBES


Intro

duct

ion Optional Accessories

FC Valve Accessories

Part Number Description21037009 PLMB PKG,200ML/MIN FC VLV 100SC PLC includes:

499541209 TUBING, .040" X 10FT, PEEKF1410153 COUPLING, 1/4-28, PVDF (QTY 2)490410255 NUT, 1/16" ANTI TWIST PEEK (P-255) (QTY 2)49041034 FERRULE, 1/16" ANTI-TWIST, PEEK (P-250) (QTY 2)21037019 TUBE,DISP .080IDX.124ODX2.440 PLC49041015 FERRULE, FLANGELESS, 1/8", TEFZEL(P-300)49041054 NUT,HEADLESS 1/4-28,1/8"TUBING(P-386)

Solvent Accessories

Part Number Description21037005 TBG ASSY,SLVT INLET4957232 INLET FILTER,20UM 5/16-24 FOR 3/16 TBG21037026 RTNR,SLVT BTL PLC21037027 BRKT, WASTE BTL PLC

Touchscreen Accessories

Part Number Description21037024 PROTECTOR,DCU SCREEN PLC21037025 STYLUS DCU


Introduction

Training

Efficient education complements your time when classroom education courses are just not feasible or convenient to schedule. Let us bring our industry-leading education to you with the use of our G.E.T. LIVE web education courses to address your PLC 2020 application questions for a single user or for multi-users.

Please contact Gilson’s Education Department with any questions and for registration details.

Gilson, Inc. 800-445-7661 608-836-1551 [email protected]

Customer Service

Gilson, Inc. and its worldwide network of authorized representatives provide customers with the following types of assistance: sales, technical support, applications, and instrument repair. If you need assistance, please contact your Gilson-authorized representative. Specific contact information can be found at www.gilson.com. To help us serve you quickly and efficiently, please refer to Before Calling Us on page 6-12.

Technical Specifications

Please be aware of the following before operating the PLC 2020 Personal Purification System.

Changes or modifications to the PLC 2020 not expressly approved by Gilson could void the warranty.

This system complies with part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) this instrument may not cause harmful interference, and (2) this instrument must accept any interference received, including interference that may cause undesired operation.

Shielded cables must be used with the system to ensure compliance with the FCC Class A limits.

mailto:[email protected]


Intro

duct

ion

Technical Specification

Definition

Connections Three USB ports for USB drive, local printer, mouse, and/or keyboard

Two inputs (contact closure, TTL), two relay outputs, and one switched +24V DC 1A output

Note: For your safety, do not switch voltages higher than 30V even though the output contacts are rated for high voltage.

Dimensions (W x D x H) 42.5 x 57.5 x 75.0 cm (17 x 23 x 30 in.)

Environmental Conditions

Indoor use

Altitude: up to 2000 m

Temperature range: 5°–40°C

Air pressure: 75–105 kPa

Humidity: Maximum relative humidity 80% for temperatures up to 31° C, decreasing linearly to 50% relative humidity at 40°C

Front Panel Touchscreen and POWER soft key/indicator light

Fuse Two 5 x 20 mm “T”-type 4.0A

Liquid Contact Materials

Valcon H, Nitronic 60 (N60), 316L stainless steel, titanium, sapphire/ceramic, ruby, PCTFE, PEEK, PTFE/HDPE, Hastelloy® C276 or Elgiloy® HT, quartz, ETFE, Delrin

Number of Racks Up to three Code 33X/34X-series racks

Power Requirements Frequency: 50/60 Hz

Voltage: 100–240V

Current rating: 2.2A for 100–120V or 1.1A for 220–240V

Safety Approvals/EMC Compliance

Certified to UL, CSA, CE, and C-Tick Safety/EMC standards

System Controller Chemical resistant 10" WSVGA TFT Touch Panel PC

5-wire resistive touchscreen

1.6 GHz Intel Atom processor

2 GB of RAM

160 GB internal hard drive

Weight 60 kg (133 lbs)

Shipping weight: 79 kg (175 lbs)

Hastelloy is a registered trademark of Haynes International, Inc. Elgiloy is a registered trademark of Elgiloy Specialty Metals


Introduction


Definition

Detection

Detector Microprocessor-controlled absorbance detector

• Dual, variable wavelengths

• Range 190–700 nm

• Spectral bandwidth: 9 nm

• Setting accuracy: ±2 nm

• Setting precision: ±0.2 nm

Drift After one hour at constant temperature: 3.0 x 10-4 AU/hour

Flow Cells The following flow cells are available:

Description Pressure

psi bar MPa

Preparative, 0.05 mm pathlength, 0.16 µL volume, quartz

500 34.5 3.45

Preparative, 0.2 mm pathlength, 0.7 µL volume, quartz

500 34.5 3.45

High pressure preparative, 0.5 mm pathlength, 2.0 µL volume, stainless steel

10,000 689.7 68.97

Lamp Warm-up Time 15–30 minutes

Lamps UV lamp: Deuterium, warranted for 750 hours or 180 days

Visible lamp: Tungsten/halogen lamp, warranted for 500 hours

Linearity 1%, when value is within sensitivity range of 0.001–2.0 AU

Peak Width 0 and 4–99 seconds

Sensitivity Range 0.001–2.0 AU. Sensitivity is adjustable in increments of 0.001 AU.

Short Term Noise

(With Air Block Installed)

4.0 x 10-5 AU; 254 nm, short term, peak to peak

5.0 x 10-5 AU; 280 nm, short term, peak to peak

The air block pathlength is set to 1 cm.

Temperature Sensitivity

3.0 x 10-4/°C for temperature change from 21° to 24°C


Intro

duct

ion


Definition

Fraction Collection

Valve Three-port, PTFE, 126.5 µL internal volume, <5 µL dead volume from common port to normally closed port

Operating pressure: 30 psi on the NO and NC port, 60 psi on the COMM port

Collection Modes Collect by Slope, Level, Time or Volume or use conditional logic collection to collect by Slope AND/OR Slope, Slope AND/OR Level, Level AND/OR Slope, or Level AND/OR Level.

Flow Rate Up to 200 mL/min

Maximum Collection Volume/Tube

40 mL (scintillation vials with Code 334 rack)

Maximum Fractions Qty Capacity Gilson Rack (Qty)

240 13 x 100 mm tubes (20 mL) Code 343(3)

144 15 x 45 mm vials (4 mL) Code 335 (3)

132 18 x 150 mm tubes (25 mL) Code 332 (3)

132 16 x 150 mm tubes (20 mL) Code 345 (3)

132 16 x 100 mm tubes (20 mL) Code 346 (3)

42 20 mL scintillation vials Code 333 (3)

42 40 mL scintillation vials Code 334 (3)

Valve Switching Speed

<20 msec

Injection

Valve Electronically-actuated manual injection valve, stainless steel

(2-position, 6-port) 0.030" ID ports, 1/16" OD

Injection Repeatability

<1% CV Total loop (3x loop size)

Sample Loops 20 µL, 50 µL, 100 µL, 250 µL, 500 µL, 1 mL, 2 mL, and 5 mL

Syringe 1 mL or 10 mL

(20 gauge disposable or reusable needles)

Valve Switching Speed

≤200 msec


Introduction


Definition

Pumping System

Composition Accuracy

±2%

Flow Rate 25SC Pump Head 1–25 mL/min

50SC Pump Head 1–50 mL/min

100SC Pump Head 1–100 mL/min

1 mL/min flow rates are for analytical scout runs and will show delays in gradient response time

Flow Rate Accuracy ±1% of requested composition

Gradient Linearity 1–99% using Pump B

Inlet Filter 20 µm

Minimum Flow Rate Adjustment

0.01% of pump head size

Mixing Volume 2.87 mL

Pressure 25SC Pump Head 4060 psi (280 bar)

50SC Pump Head 2030 psi (140 bar)

100SC Pump Head 1015 psi (70 bar)

Solvent Selection Valve

One outlet and four inlets to select one solvent out of four

Inlet: 216 µL internal volume total per channel

Outlet: 131 µL internal volume total per channel with 63 µL in common path

Total valve internal volume: 1451 µL

<5 µL dead volume per channel


Intro

duct

ion Operating Environment

You can use the system in normal laboratory and cold room environments.

The factors described below will influence the performance of your system.

Temperature

Changes in ambient temperature can produce noticeable detector output variations, especially at higher sensitivity settings.

Avoid areas which are in direct sunlight for part of the day and those which are subject to draft; for example, do not place the system near an open window or doorway.

Contaminants

Smoke, vapors, and acid fumes can cause baseline drift and noise, and may damage the system.

Ventilation

You must allow at least 4 inches (10 cm) of clearance behind the system to permit exhaust from the cooling fan to escape.


Chapter 3

Contents

Shipping Screw Removal ......................................................... 3-2

Pump Head Installation ............................................................ 3-3

Flow Cell Installation ................................................................. 3-4

Injection Port Installation ........................................................ 3-5

Tubing Clip Installation ............................................................ 3-5

Rack Installation .......................................................................... 3-5

Rear Panel Connections ........................................................... 3-6

Accessory Installation .............................................................3-10

Plumbing Connections ..........................................................3-11

Startup..........................................................................................3-17

Software Operation .................................................................3-17

Priming the PLC 2020 ..............................................................3-17

Conditioning the Pump Heads ............................................3-18

Shut Down the PLC 2020 .......................................................3-18

Setup/Operation


Setu

p/Op

erat

ion Shipping Screw Removal

All four shipping screws must be removed before operating the system. There are holes in the rear panel (below the analog outputs) for storing the screws.

Tools Required

• 3 mm Allen wrench (included with the Unpacking Instructions)

• Phillips screwdriver (not included)

The screw that holds the panel door closed during shipment is located to the left of the injection valve, behind the panel door, and near the run light cable. The head of the screw is accessible from the right side of the system.

1 Use the 3 mm Allen wrench to remove the screw.

2 Store the screw in the rear panel of the system.

The screws that secure the detector optical assembly are located on the bottom of the detector.

The system will not operate properly if these three screws are not removed.

1 Use the 3 mm Allen wrench to remove the two screws that secure the detector to the system.

2 Pull out the detector.

Pull the detector out only until the middle set of rubber feet are resting on the edge of the enclosure.

3 Remove the three Phillips screws (with three washers each) from the bottom of the detector.

4 Slide the detector back into position and replace the two screws that secure the detector.

5 Store the screws and washers in the rear panel of the system.

Reach below the detector from the right side to access the

panel door screw (not shown)

Remove these screws to access

the three shipping screws (not shown)

Remove the three Phillips screws from the bottom of

the detector


Setup/Operation

Pump Head Installation

1 Orient the pump head so the notch is at the bottom and then place it over the pin on the bottom of the pump.

2 Hold the pump head in place with one hand and place the clamp diagonally over the head.

3 Turn the clamp clockwise into position in the slots on both sides of the pump head.

4 Tighten the thumbscrew until the clamp holds the pump head securely. Make sure that the clamp ends are secured in their slots on both sides.

pin


Setu

p/Op

erat

ion Flow Cell Installation

Be extremely careful when working with the flow cell and its fittings. Flow cells are not covered by warranty if damaged during installation.

1 Open the panel door.

2 Use the 5/32" Allen wrench included in the accessory package to remove the two screws on the front of the detector block.

3 Pull the detector block toward you and then slide it to the side.

4 Insert the flow cell assembly into the detector and tighten the flow cell assembly screw using a Phillips screwdriver.

Note: The high pressure flow cell assembly uses the 7/64" Allen wrench included with the flow cell assembly.

5 Replace the detector block and tighten its two screws. Do not pinch the outlet tubing.

6 Close the panel door.

Remove these screws from the front of the detector

block to install the flow cell

flow cell assembly

screws

high pressure preparative flow cell

flow cell assembly

screw

preparative flow cell

outlet tubing


Setup/Operation

Injection Port Installation

1 Insert a needle into the injection port.

2 Connect the injection port to port 1 on the injection valve and finger tighten.

3 Remove the needle and then insert it in the injection port again.

Note: When reinserted, the needle should encounter slight resistance before being fully installed.

Connect the syringe to the needle to perform injections.

Tubing Clip Installation

Three adhesive-back tubing clips are included with the solvent inlet tubing. Install one clip on the side to retain the waste tubing. Install two clips on the rear panel to retain the solvent inlet tubing. Up to four 4 L bottles can be placed on top of the system.

Rack Installation

1 Orient the rack so that the code number is facing forward.

2 Locate the middle slot on the back of the rack. Slide this over the pin on the tray.

3 Fit the middle slot on the front of the rack over the pin in the front of the tray.


Setu

p/Op

erat

ion Rear Panel Connections

1 Analog output channels

2 Fuse drawer

3 Power switch

4 Power receptacle

5 Input ports

6 Output ports

7 Gilson Serial Input/Output Channel (GSIOC) port

8 Ethernet ports

9 USB ports

POWER

FUSE

MAINS

SHOWNIN OFF

POSITION

120-240V~/2.0A220-240V~/1.0A

50/60 HZ

FUSE

250V, 4.0A "T"(5 x 20mm)-(2)

THIS DEVICE COMPLIES WITH PART 15 OF THE FCC RULES. OPERATION IS SUBJECTTO THE FOLLOWING TWO CONDITIONS: (1) THIS DEVICE MAY NOT CAUSE HARMFULINTERFERENCE, AND (2) THIS DEVICE MUST ACCEPT ANY INTERFERENCE RECEIVED,INCLUDING INTERFERENCE THAT MAY CAUSE UNDESIRED OPERATION.

WARNING: DISCONNECT SUPPLY BEFORE SERVICING.ADVERTISSEMENT: COUPLER L'ALIMENTATION AVANT L'ENTRETIEN ET LE DEPANNAGE.

AVERTISSEMENT: POUR UNE PROTECTIONCONTINUE CONTRE LE RISQUE D’INCENDIE,REMPLACER LE FUSIBLE UNIQUEMENT PARUN FUSIBLE DE MEME TYPE ET DE MEMECOURANT NOMINAL.

CAUTION: FOR CONTINUEDPROTECTION AGAINST RISK OFFIRE, REPLACE ONLY WITH THESAME TYPE AND RATING OF FUSE

SEE USER’S GUIDE FORSAFETY AND SERVICE CONCERNS

!

25N959

UL 61010-1CAN/CSA-C22.2 61010-1NRTL TÜV Rheinland

EN 61010-1

OUTPUT{1 21+24V DC (1A MAX)

65

-+12

43

INPUT{ A 21

B

43

ETHERNETGSIOC USB

MADEIN USA

GILSON, INC.MIDDLETON, WI 53562www.gilson.com

PLC 2020

PRIMARYCHANNEL

GROUND

MONITORCHANNEL

GROUND

6 985

7

4

3

2

1


Setup/Operation

Analog Output Channels

The system has two output channels, Primary and Monitor.

USB Ports

The system has one USB port on the front and two USB ports on the rear panel.

The USB ports can be used to connect to any of the following options: USB drive, local printer, keyboard, and/or mouse.

Print to Local Printer

Reports and chromatograms can be printed from the PLC 2020 software. The software provides for setting up to print to a local printer or saving the information to a .PDF that can be saved to a USB drive. The Settings - Printer tab enables setting up to print to a local printer.

1 Copy printer driver to a USB drive and then connect the USB drive to a USB port on the PLC 2020.

2 Connect a USB printer to a USB port on the back of the PLC 2020 and then turn the printer on. The Found New Hardware wizard should appear.

Note: If the printer is not detected, tap Install Printer to install the printer using the Add Printer wizard on the Settings - Printer tab.

3 Select Install from a list or specific location (Advanced) and then tap Next.

4 Select Search for the best driver in these locations.

5 Clear the check box for Search removable media (floppy, CD-ROM...).

6 Select the check box for Include this location in the search.

7 Tap Browse and then browse for the folder containing the printer driver on the USB drive.

8 Tap Next and then tap Finish. The printer appears in the list of available printers.

9 On the Settings - Printer tab, tap to select the printer to use.

10 Tap Set as Default.

11 From the drop-down list next to Page Size, select the page size to use for all reports or chromatograms printed from the PLC 2020 software.

12 Tap to select the orientation to use for all reports or chromatograms printed from the PLC 2020 software: Portrait or Landscape.


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ion Ethernet and GSIOC Ports

Not supported in this release.

Input and Output Ports

You can use the input and output contacts on the rear panel of the system to control peripheral devices. Refer to Rear Panel Connections on page 3-6 for the location of the input and output ports.

Inputs

The input terminal block on the system has four contacts. All of the inputs are paired, and each pair include a GROUND reference ( ).

The contact input pairs are labeled A and B.

A contact is connected if it has a short across the input or is held low by a TTL output or other device.

Never connect voltages higher than 5V DC to an input. When using TTL signals, be sure to match GROUND connections.

Outputs and DC Power Outputs

The output terminal block has six contacts.

Pins 1 through 4 are paired, isolated-relay contact closures and are labeled 1 and 2.

Pins 5 and 6 are DC power outputs and can be turned on (supplying +24V DC) or off (+24V DC floats) via software control.

Make Connections

To make connections, you will need the following:

• 2-conductor cable (22–30 gauge for each wire)

You can purchase a 6-foot piece of suitable cable (part number 709910206) or a package of 5 cables with identification markers (part number 36078155) from Gilson.

• wire insulation stripper

• small-blade screwdriver


Setup/Operation

To make connections with the 2-conductor cable:

1 Cut the cable into pieces of appropriate length.

2 Strip about 3 mm of insulation from each end of the cable.

3 Insert each wire into the appropriate terminal on the terminal block connector.

When making connections, be sure to maintain the correct orientation of the connector relative to the port.

Push the wire all the way in; then tighten its corresponding pin screw.

4 Push the terminal block connector in as far as it will go. It is designed to fit snugly into its receptacle.

5 Connect the opposite ends of the wires to the other device(s). Be sure to match ground connections.

6 Label each cable to identify the purpose of the connection.

Power Cord Connection

Locate the appropriate power cord for your line voltage. Use the power cord to connect the system to a power source.


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ion Accessory Installation

Column Holder Bracket and Waste Bottle Bracket Installation

Install the column holder bracket (included in the accessory package) and the waste bottle bracket (ordered separately, part number 21037027) on the right side of the system.

To install the column holder bracket, place the large holes on the bracket over one of the top two sets of pins on the right side of the system and then slide the bracket into place. (Additional column holder brackets are available, part number 21031063.)

To install the waste bottle bracket, place the large holes on the bracket over the lower set of pins on the side of the system and then slide the bracket into place.


Setup/Operation

Plumbing Connections

The overall plumbing diagram is shown below. The tables and diagrams on the following pages provide detailed information about making these connections.

23

4

56

1

inlet

outlet

Pump A Pump B

dampener

PPMM

�lter

to waste

injection valve

FC valve

inletoutlet

inlet

outlet

from solvent bottle from solvent bottle

to waste

to waste

drains100SC

25/50SC

1 2

3 4

L

P

O

N

K

J

H

E

D

C

A

Q

�ow cell

B

to wasteM

column

F

couplingcoupling

PEEKtubing

coilG

inlet

outlet

I

Note: The end of the waste tubing should be above the level of the liquid in the waste container.


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ion Plumbing Connections (A–H)

Tubing Connections

A Port 1 on the injection valve (INJECTION PORT), refer to Injection Port Installation on page 3-5

BPort 2 to port 5 on the injection valve

Sample loop (ordered separately) Use the supplied fittings on the sample loop to connect to ports 2 and 5.

C

Port 3 on the injection valve from column inlet

Uses these parts from PLMB PKG, INJ VLV PLC (PN 21037010): TUBING, 0.030" X 5FT, PEEK

VALCO PEEK NUT AND FERRULE 1/16 orTUBING, 0.030" ID X 5FT, SS VALCO SS NUT AND FERRULE F-100N FINGERTIGHT FITTING

PEEK: On one end of the PEEK tubing coil use a PEEK nut and ferrule to connect to port 3 on the injection valve. Cut the tubing to the appropriate length. OrStainless steel: On one end of the stainless steel tubing use a stainless steel nut and ferrule to connect to port 3 on the injection valve. Cut the tubing to the appropriate length using a stainless steel tubing cutter.On the other end of the tubing use the F-100N fingertight fitting to connect to the column inlet.

D Port 4 on the injection valve from 10 µm in-line filter (PRE-INSTALLED)

E

Port 6 on the injection valve to waste

Uses these parts from PLMB PKG, INJ VLV PLC: VALCO PEEK NUT AND FERRULE 1/16

TUBING, 0.5MM ID X 1.5MM OD, TFE 10 FT

On one end of the tubing use a PEEK nut and ferrule to connect to port 6 on the injection valve. Place the other end of the tubing in a waste container.

F

Column outlet to flow cell inlet

Inlet tubing pre-installed on the flow cellF-100N FINGERTIGHT FITTING (located in PLMB PKG, INJ VLV PLC)

Route the inlet tubing out the slot on the right side of the system. Use the F-100N fingertight fitting to connect the stainless steel inlet tubing to the column outlet.

G

Flow cell outlet to FC valve

Outlet tubing pre-installed on the flow cell

Route the end of the outlet tubing through the grommet on the lower left side of the detector enclosure and connect it to the coupling from the tubing assembly connected to the IN port on the FC valve.

Note: If you ordered the PLMB PKG,200ML/MIN FC VLV 100SC PLC (part number 21037009) refer to Plumbing Connections for the FC Valve (Optional) on page 3-15 for instructions about making this connection.

H FC valve to waste (PRE-INSTALLED and routed through the drain)

23

4

56

1

�lter

to waste

injection valve

FC valve

inletoutlet

inlet

outlet

to waste H

E

D

C

Aow cell

B

column

F

couplingcoupling

PEEKtubing

coilG


Setup/Operation

Plumbing Connections (I–K and O)

I

Solvent bottle to inlet of Pump A

Uses these parts from PLMB PKG, SLVT SEL VLV PLC (PN 21037013): TBG ASSY,SLVT INLET

INLET FILTER,20UM 5/16-24 FOR 3/16 TBG FTG,5/16-24 TO 1/4-28 M PEEK ADPTR or FTG,5/16-24 F TO M10x1.5 PEEK ADPTR

Connect the fitting (PN 49010000 [25SC] or PN 49010001 [50/100SC]) to the inlet port of Pump A. Connect the end of the tubing assembly with the fitting attached to the fitting on the inlet of Pump A. Connect the other end of the tubing to the inlet filter using the fittings included with the filter. Place the inlet filter in the solvent bottle.

J

25/50SC pump head—Outlet of Pump A to 25/50SC on dampener (bottom left port, as shown in diagram)100SC pump head—Outlet of Pump A to 100SC on dampener (top left port, not shown)

Uses these parts from PLMB PKG, PMP PLC (PN 21037011) TBG ASSY,25/50 SC PMP “A” TO DMPNR PLC

orTBG ASSY,100 SC PMP “A” TO DMPNR PLC WRENCH, 5/16 - 1/4

Note: All SS tubing included in PLMB PKG, PMP PLC is pre-formed with the fittings attached, but not swaged; be careful not to lose the fittings.

One end of the tubing assembly is slightly longer than the other. Remove the cover from the longer end of the tubing and place the loosely assembled ferrule and tubing into the appropriate port on the dampener (until the tubing is bottomed in the port). While pressing down firmly on the tubing, finger tighten the nut and then use the wrench to tighten an additional 3/4 of a turn to swage the ferrule onto the tubing. Remove the tubing and fittings from the port. Repeat this procedure with the shorter end of the tubing in the outlet port of Pump A. After swaging both ferrules onto the tubing, connect one end to the outlet of Pump A and the other end to the appropriate port on the dampener.

K

25/50SC pump head—25/50SC on dampener to Pressure, Purge, and Mixing Module (PPMM, as shown in diagram)100SC pump head—100SC on dampener to PPMM (not shown)

Uses these parts from PLMB PKG, PMP PLC: TBG ASSY,25/50 SC DMPNR TO PPMM PLC or

TBG ASSY, 100 SC DMPNR TO PPMM PLC WRENCH, 5/16 - 1/4

Refer to the diagram for the correct orientation of the tubing. Using the process described in J , swage the ferrules on each end of tubing and then install the tubing.

L – P are shown on the next page.

Q

Front drains to waste

TUBE,VITON 0.313IDx0.438OD 10FT (located in PLMB PKG, PMP PLC)

Cut the tubing to the appropriate lengths. Connect one end of the tubing to the barbed adapter on the front of the system and place the other end of the tubing in a waste container. The drain on the left is from the tray and the drain on the right is the main drain.

inlet

outlet

Pump A

dampener

PPMM

from solvent bottle

to waste

drains100SC

25/50SC

1 2

3 4

K

JQ

I


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ion Plumbing Connections (L–P)

L PPMM to 10 µm in-line filter (PRE-INSTALLED)

M

PPMM to waste

TBG ASSY, PPMM TO WASTE PLC (located in PLMB PKG, PMP PLC)

Connect the end of the tubing with the fitting to the front port on the top of the PPMM. Place the other end of the tubing in a waste container.

N

Solvent valve block to inlet of Pump B

Uses these parts from PLMB PKG, SLVT SEL VLV PLC: TBG ASSY,SLVT VLV TO PMP “B”

FTG,5/16-24 TO 1/4-28 M PEEK ADPTR or FTG,5/16-24 F TO M10x1.5 PEEK ADPTR EXTND TOOL,5/16-24 DIBA FTG

Connect the fitting (PN 49010000 [25SC] or PN 49010001 [50/100SC]) to the inlet port of Pump B. Connect one end of the tubing assembly to the fitting on the inlet of Pump B. Connect the other end of the tubing assembly to the center port on the solvent valve block. Tighten using the supplied tool.

O

Solvent bottle to solvent valve block

Uses these parts from PLMB PKG, SLVT SEL VLV PLC: TBG ASSY,SLVT INLET

INLET FILTER,20UM 5/16-24 FOR 3/16 TBG EXTND TOOL,5/16-24 DIBA FTG

Connect the end of the tubing assembly with the fitting attached to a port (1–4) on the solvent valve block. Tighten using the supplied tool. Connect the other end of the tubing assembly to the inlet filter using the fittings included with the filter. Place each inlet filter in the appropriate solvent. (The solvent inlet tubing can be routed below the system and then through the cable clips on the back.) Note: Inlet tubing for one solvent is supplied. To use up to three additional solvents, purchase additional solvent inlet tubing assemblies (21037005) and inlet filters (4957232).

P

Outlet on Pump B to PPMM

TBG ASSY,PMP “B” TO PPMM PLC (located in PLMB PKG, PMP PLC)

Refer to the diagram for the correct orientation of the tubing. Using the process described in J , swage the ferrules on each end of tubing and then install the tubing.

Pump B

dampener

PPMM

�lter

from solvent bottle

1 2

3 4

L

P

O

N

to wasteM

inlet

outlet

Note: The end of the waste tubing should be above the level of the liquid in the waste container.


Setup/Operation

Plumbing Connections for the FC Valve (Optional)

The following instructions apply to using the PLMB PKG,200ML/MIN FC VLV 100SC PLC (ordered separately, part number 21037009).

The following items are included in this package.

Part Number Description499541209 TUBING, 0.040" X 10FT, PEEKF1410153 COUPLING, 1/4-28, PVDF (QTY 2)490410255 NUT, 1/16" ANTI TWIST PEEK (P-255) (QTY 2)49041034 FERRULE, 1/16" ANTI-TWIST, PEEK (P-250) (QTY 2)21037019 TUBE,DISP 0.080IDX0.124ODX2.440 PLC49041015 FERRULE, FLANGELESS, 1/8", TEFZEL(P-300)49041054 NUT,HEADLESS 1/4-28,1/8"TUBING(P-386)

Refer to the diagram and table when making connections using this plumbing package.

Tubing Connections

7 Flow cell outlet to FC valve

Outlet tubing pre-installed on the flow cellUses these parts from PLMB PKG,200ML/MIN FC VLV 100SC PLC (PN 21037009): TUBING, 0.040" X 10FT, PEEK

COUPLING, 1/4-28, PVDF (2) NUT, 1/16" ANTI TWIST PEEK (P-255) (2) FERRULE, 1/16" ANTI-TWIST, PEEK (P-250) (2)

Route the end of the outlet tubing through the grommet on the lower left side of the detector enclosure.Remove the pre-installed 0.030" PEEK tubing coil and two couplings connected to the PEEK tubing from the FC valve.On both ends of the 0.040" PEEK tubing coil, use a P-255 nut and P-250 ferrule to connect to a coupling. Connect one end to the outlet tubing from the detector. Connect the other end to the PEEK tubing from the FC valve.

8 FC valve to waste (PRE-INSTALLED and routed through the rinse station/waste drain)

FC valve

inlet

outlet

to waste 8

�ow cell

couplingcoupling

PEEKtubing

coil7


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ion Fraction Collection Probe Installation

To replace the pre-installed fraction collection probe with the probe included in this package:

1 Slide the valve to the front of the Y-arm.

2 Use a small Phillips screwdriver to remove the two screws that secure the valve to the bracket.

3 Remove the original fraction collection probe and fittings and replace with the new ones.

4 Use the Phillips screwdriver to reattach the valve to the bracket.

5 Ensure that the fraction collection probe is in the correct position. The probe should be perpendicular to the bottom of the Y-arm and centered in the slot on the Y-arm.


Setup/Operation

Startup

Turn on the MAINS power switch on the rear panel to power on the system. The power indicator light changes to green, the detector lamps turn on and begin to warm up, the fraction collection probe homes, and the software starts.

Note: Before operating the system, prime the pump heads and, if necessary, condition the pump heads.

Software Operation

The PLC 2020 Personal Purification System includes built-in software control. The software provides fraction collection capabilities including conditional logic collection.

The PLC 2020 software’s interface features graphical gradient creation and adjustments, tap-and-drag, icon-based tasks, and graphical method optimization.

For more information about the software, refer to its on-line help and the PLC 2020 Personal Purification Software Quick Reference Guide supplied with the system.

Priming the PLC 2020

The unit needs to be powered on and a solvent valve position must be selected before priming. Use Manual Control to set the solvent valve position.

1 Disconnect the waste tubing from the PPMM.

2 Connect the priming syringe (part number 21037021) to the outlet of the PPMM.

3 Open the prime purge valve and aspirate with the syringe.

4 Disconnect the syringe and dispense any aspirated liquid into a waste container.

5 Repeat steps 2–4 until the tubing connected to both Pump A and Pump B is filled with liquid.


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ion Conditioning the Pump Heads

To prolong the life of the piston seal, the pump heads should be conditioned, especially if there will be continuous pumping at maximum flow rates.

Gilson recommends performing the conditioning procedure at the following times:

• after pump head installation

• after pump head replacement

• after piston seal replacement

Contact your Gilson-authorized representative for the PLC 2020 Personal Purification System conditioning procedure.

Shut Down the PLC 2020

Do not turn off the MAINS power switch without first following this procedure.

1 Press the POWER button on the front of the system. Optionally, save any unsaved information.

The power indicator light changes to orange, the pumps turn off, the detector lamps turn off, and the software display shuts down.

The fan in the lower portion of the PLC 2020 will continue running until the MAINS power switch is turned off.

2 Turn off the MAINS (power) switch on the rear panel.


Contents

Helpful Hints ................................................................................ 4-2

System ............................................................................................ 4-2

Touchscreen ................................................................................. 4-3

Fluid Path ....................................................................................... 4-4

Cleaning the Inside of the Flow Cell .................................... 4-6

Unclogging the Flow Cell ........................................................ 4-8

Chapter 4Cleaning


Clea

ning Helpful Hints

In order to keep your PLC 2020 Personal Purifications System at peak performance, it is recommended that you do the following:

• Change or clean the tubing regularly to maintain maximum performance.

• Check periodically to ensure that all fittings are tight.

• Wipe up all spills immediately.

• Warm fluids to room temperature before running them through the system; cold fluids may cause leakage.

System

The system should be cleaned occasionally using a dry, clean cloth. Or, if necessary, use a cloth dipped in soapy water. If liquid is accidentally spilled on the system, wipe the system using a dry, clean cloth.


Cleaning

Touchscreen

Under normal use, dirt and fingerprints do not affect the operation of the touchscreen on the display. However, it is recommended to periodically clean the touchscreen for the best visual and operational experience.





3 Disconnect the power cord from the power outlet and from the rear panel receptacle.

Clean the Touchscreen

Normal household cleaners, cloths, and wipes may damage sensitive electronic components.

Do not use any solutions that contain ammonia, acidic, alkali, or other caustic chemicals on the touchscreen.

Do not use any vinegar-based solutions.

Do not spray cleaner directly on the touchscreen.

1 Spray a small amount of LCD and plasma screen cleaner or isopropyl alcohol onto an LCD and plasma screen cleaning wipe or electronic equipment wipe. (Do not spray cleaner directly on the touchscreen.)

To prevent injury, observe good laboratory practices when handling solvents. Know the physical and chemical properties. Refer to the Material Safety Data Sheet for the solvent used.

2 Wipe the touchscreen in a gentle motion to remove any dirt, dust, or fingerprints.

3 Use a dry LCD and plasma screen cleaning wipe or electronic equipment wipe to remove any excess moisture.


Clea

ning Fluid Path

Depending upon your use of the system, it may be necessary to flush the entire fluid path.

It is important to clean the fluid path if you will not be using the system for a while or if you are using a solution with a high salt concentration. Refer to the following instructions.

To prevent injury, observe good laboratory practices when handling solvents. Know the physical and chemical properties. Refer to the Material Safety Data Sheets for all solvents used.

1 Prime the fluid path with distilled or deionized water.

2 Flush the fluid path with 30% ethanol. The fluid path has now been cleaned appropriately for weekend storage (or longer).

3 Prime and flush the fluid path with distilled or deionized water before running applications.

Cleaning Methods

Depending on the samples or reagents that come into contact with the fluid path, you may need to vary your cleaning methods accordingly. Use the following cleaning protocols as references and make any changes to them as required for the samples and reagents being pumped for your application.

Proteins and Peptides



2 Flush the fluid path using a weak detergent solution.

3 Pause the priming sequence.

4 After 30 minutes, resume flushing and priming the fluid path using distilled or deionized water to pump the remaining detergent from the tubing into a waste container.

5 When you are satisfied that the entire fluid path has been flushed with water, end the priming sequence.


Cleaning

Acidic Compounds, Basic Compounds, or Salt Solutions



2 Flush the fluid path using a 0.1N NaOH solution.


4 After 10 minutes, resume priming the fluid path using distilled or deionized water. Prime until the fluid path has been flushed with water.


6 Prime the fluid path using a 0.1N HCl solution.


8 After 10 minutes, resume priming the fluid path using distilled or deionized water.


Biological Fluids



2 Make a solution of 10% bleach by adding one part of commercial bleach to nine parts of water.

3 Flush the fluid path using the bleach solution.


5 After 30 minutes, resume priming the fluid path using distilled or deionized water to pump the remaining bleach solution from the tubing into a waste container.



Clea

ning Cleaning the Inside of the Flow Cell

Be extremely careful when working with the flow cell and its fittings. Flow cells are not covered by warranty if damaged during any maintenance procedure.

There are two ways to clean an active flow cell that has become coated with light absorbing material: solvent cleaning and acid cleaning.

• If you suspect that droplets of an organic-soluble contaminant or immiscible solvent contaminate the active flow cell, draw or pump a series of miscible solvents through the cell. Refer to Solvent Cleaning.

• If you suspect that residual proteins contaminate the flow cell, draw 50% nitric acid through the cell. Acid cleaning is a more thorough technique, though much more care must be taken. Refer to Acid Cleaning.

Solvent Cleaning


Refer to the table for the list of solvents and the order that they will be used when cleaning the inside of the flow cell. The solvents are based on the type of mobile phase pumped, polar or non-polar.

Mobile Phase

Polar Non-Polar

Solv

ents

1 methanol

2 tetrahydrofuran

3 methylene chloride

4 methanol

1 hexane

2 isopropanol

3 methanol

4 water

1 Disconnect the inlet and outlet tubing from the system.

2 Using a 1/4-28 coupler, connect a Luer-lock fitting to the fitting on the outlet tubing. Then attach a large glass syringe to the Luer-lock fitting.

3 Place the end of the inlet tubing into a reservoir of the first solvent of the series. Refer to the table for the list of solvents.

4 Draw solvent through the cell.

5 Repeat for each remaining solvent in the series.


Cleaning

6 Reconnect the inlet and outlet tubing to the system.

7 Check the detector performance by running a sample through the system.

Acid Cleaning

Take all necessary precautions so that acid does not come into contact with eyes, skin, clothing, and equipment surfaces.

1 Disconnect the inlet and outlet tubing from the system.

2 Using a 1/4-28 coupler, connect a Luer-lock fitting to the fitting on the outlet tubing. Then attach a large glass syringe to the Luer-lock fitting.

3 Place the end of the inlet tubing into a reservoir of 50% nitric acid.

4 Draw 50% nitric acid through the cell.

Use nitric acid. Do not use halogenated acid. Halogenated acid will damage stainless steel.

Do not pump acid through the cell. Never put acid under pressure.

5 After the acid flush, draw HPLC-grade water through the cell.

6 To be sure that all acid has been removed, monitor the pH of the final outflow.

7 Reconnect the inlet and outlet tubing to the system.

8 Check the detector performance by running a sample through the system.


Clea

ning Unclogging the Flow Cell

To try to eliminate a blockage in the active cell or in its inlet or outlet tubing, pump solvent backwards through the flow cell.

1 Connect the mobile phase pumps to the detector outlet.

a) Locate the coupling in the left side of the detector enclosure that connects the tubing assembly to the detector outlet.

b) Disconnect the tubing assembly fitting from the coupling, leaving the detector outlet fitting connected to the coupling.

c) Disconnect the F-100N fingertight fitting from the column inlet and remove the fitting from the tubing.

d) Replace the fingertight fitting with a P-201 black nut (PN 49041012) and P-200R red ferrule (PN 49041011).

e) Connect the P-201 black nut to the coupling in the detector enclosure.

2 Disconnect the detector inlet tubing from the column and direct the inlet to a waste container.

3 Using Manual Control in the PLC 2020 software, start flow of the mobile phase pumps at 0.1 mL/min. Monitor the system pressure, stopping the pumps immediately if the system pressure exceeds 500 psi (34 bar).

4 Gradually increase the flow rate in increments of 0.1 mL/min, continuing to monitor the system pressure.

A significant pressure drop indicates that the blockage has been removed.


Contents

Tubing ............................................................................................ 5-2

Detector ......................................................................................... 5-4

Pumping System .......................................................................5-15

Fraction Collector .....................................................................5-25

Injection Valve ...........................................................................5-28

Fuse ...............................................................................................5-32

Additional Replacement Parts .............................................5-32

Chapter 5Replacing Parts


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rts Tubing

It is important to keep all tubing clean and free of crimps. Replace tubing as needed.

Stainless Steel Tubing and Fittings

When retightening a nut after the ferrule is swaged to the tubing, 1/6 turn past fingertight is sufficient to reconnect. Monitor the connection for any leaks. If leaking occurs, continue to tighten the nut a little at a time until the leak stops. If it is necessary to tighten the nut more than one complete revolution past finger tight, the fitting should be replaced, as excessive tightening is typically indicative of a damaged product.

Part Number Description21037011 PLMB PKG,PMP PLC21037000 TBG ASSY,25/50 SC PMP “A” TO DMPNR PLC21037001 TBG ASSY,25/50 SC DMPNR TO PPMM PLC21037015 TBG ASSY,100 SC PMP “A” TO DMPNR PLC21037016 TBG ASSY,100 SC DMPNR TO PPMM PLC21037002 TBG ASSY,PMP “B” TO PPMM PLC21037008 TBG ASSY,PPMM TO WASTE PLC47014386301 TUBE,VITON .313IDx.438OD 10FT21037021 SYRINGE, PRIME PLC36610101 WRENCH, 5/16 - 1/4

Other Tubing and Fittings

Injection Valve Plumbing

Part Number Description21037010 PLMB PKG,INJ VLV PLC

49953059 TUBING, .030" X 5FT, PEEK4903180411 VALCO PEEK NUT 1/16, 1 EA4903180511 VALCO PEEK FERRULE 1/16, 1 EA 499331519 TUBING, .030" ID X 5FT, SS490318011 VALCO NUT SS,ZN1,PKG OF 10490318021 VALCO FERRULE SS,ZF1S6,PKG OF 1049041001 FITTING,FINGERTIGHT,10-32,KEL-F(F-100N) 495033 TUBING, .5MM ID X 1.5MM OD,TFE 10 FT/PK49440007 SAMPLE LOOP, 20UL, SS, VALCO, 1/16"49440008 SAMPLE LOOP, 50UL, SS, VALCO, 1/16"49440009 SAMPLE LOOP, 100UL, SS, VALCO, 1/16"


Replacing Parts

Part Number Description494400002 SAMPLE LOOP, 250UL, SS, VALCO, 1/16"494400005 SAMPLE LOOP, 500UL, SS, VALCO, 1/16"49440001 SAMPLE LOOP, 1ML, SS, VALCO, 1/16"49440002 SAMPLE LOOP, 2ML, SS, VALCO, 1/16"49440005 SAMPLE LOOP, 5ML, SS, VALCO, 1/16"54350100 SYRINGE,10CC LUER LOK PLASTIC 100PK54350101 SYRINGE,1CC LUER LOK PLASTIC 100PK54350302 NDL,20GA 1" LUER LOK W/TIP GUARD 100PK54350303 NDL,20GA 1" LUER LOK BLUNT TIP REUSE 3PK

Solvent Selection Valve Plumbing

Part Number Description21037013 PLMB PKG,SLVT SEL VLV PLC21037005 TBG ASSY,SLVT INLET21037003 TBG ASSY,SLVT VLV TO PMP “B”49010000 FTG,5/16-24 TO 1/4-28 M PEEK ADPTR 49010001 FTG,5/16-24 F TO M10x1.5 PEEK ADPTR54096003 CLA,CBL1.18 DIA ADH BACKED RICHCO SHR-C354118051 SPIRAL WRAP,1/2" BLACK21037022 EXTND TOOL,5/16-24 DIBA FTG

Fraction Collection Valve Plumbing

Part Number Description49953059 TUBING, .030" X 5FT, PEEKF1410153 COUPLING, 1/4-28, PVDF490410255 NUT, 1/16" ANTI TWIST PEEK (P-255)49041034 FERRULE, 1/16" ANTI-TWIST, PEEK (P-250)21037009 PLMB PKG,200ML/MIN FC VLV 100SC PLC499541209 TUBING, .040" X 10FT, PEEKF1410153 COUPLING, 1/4-28, PVDF490410255 NUT, 1/16" ANTI TWIST PEEK (P-255) 49041034 FERRULE, 1/16" ANTI-TWIST, PEEK (P-250)


Repl

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rts Detector

Flow Cell Assembly


Refer to the instructions in this section when replacing the entire flow cell assembly. To replace tubing and/or the flow cell individually, refer to Tubing and/or the Flow Cell on page 5-6.

The table contains part numbers for replacement flow cell assemblies.

Part Number Description100441 CELL BLOCK ASSY, 0.2MM, QUARTZ100443 CELL BLOCK ASSY, 0.05MM, QUARTZ1004395 FLOW CELL, PREP HIGH PSI, 2 UL, .5MM






Replace the Flow Cell Assembly

1 Open the panel door to access the detector.

2 Use the 5/32" Allen wrench included in the accessory package to remove the two screws on the front of the detector block.





Replacing Parts

4 Use a Phillips screwdriver to remove the flow cell assembly screw. Then pull the flow cell assembly toward you to remove it.

Note: The high pressure flow cell assembly has two screws that can be removed using the 7/64" Allen wrench included with the flow cell.

5 Insert the new flow cell assembly into the detector and tighten the flow cell assembly screw (the high pressure flow cell assembly has two screws).



flow cell assembly

screws

high pressure preparative flow cell

flow cell assembly

screw

preparative flow cell

outlet tubing


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rts Tubing and/or the Flow Cell


Refer to the instructions in this section when replacing tubing and/or the flow cell individually. To replace the entire flow cell assembly, refer to Flow Cell Assembly on page 5-4.

Note: The instructions in this section do not apply to the high pressure flow cell assembly.






Remove the Flow Cell Assembly


2 Use the 5/32” Allen wrench included in the accessory package to remove the two screws on the front of the detector block.





Replacing Parts

4 Use a Phillips screwdriver to remove the flow cell assembly screw. Then pull the flow cell assembly toward you to remove it.

Refer to the following procedures and diagrams when replacing the flow cell and/or tubing.

Remove the Flow Cell

1 Loosen the fittings for the inlet and outlet tubing. The inlet tubing enters the flow cell assembly from the bottom while the outlet tubing exits from the top.

2 Using a 1/16" Allen wrench, loosen the screw located on the left side of the flow cell assembly.

3 Carefully remove the flow cell using a pair of tweezers or by tilting the flow cell assembly until the flow cell drops into the palm of your hand.

Notice that there is only one way that the flow cell fits into the flow cell assembly.

circular portion

screw

inlet tubing

flow cell assembly screw

inlet tubing clamp

flow cell

outlet tubing


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rts Replace the Flow Cell

Refer to the instructions in this section when replacing the flow cell. The table contains part numbers for replacement flow cells.

Part Number Description100401 FLOW CELL, PREP, .7UL, .2MM, QUARTZ100407 FLOW CELL, PREP, 0.16UL, 0.05 MM, QUARTZ

1 Before inserting the replacement flow cell, dampen the quartz windows with alcohol or a laboratory cleaning solution. Then wipe the windows with a piece of lens paper or lint-free towel.

2 Wipe the inside of the flow cell mounting area with a lint-free towel soaked in alcohol.

3 Carefully dry all surfaces of the assembly.

4 Without touching the quartz windows, insert the flow cell into its chamber. Push the cell into the chamber as far as it will go. A poorly aligned flow cell may restrict or block flow.

5 Use a 1/16" Allen wrench to tighten the screw located on the left side of the flow cell assembly.

Do not over tighten this screw. Over tightening may crack the flow cell.

Replace the Outlet Tubing

Refer to the instructions in this section when replacing the outlet tubing (OUTLET TUBING, PREP, QUARTZ, 118/119, part number 1007415).

1 Remove the outlet tubing and fitting from the flow cell assembly.

2 Connect the fitting on the replacement tubing to the flow cell assembly. Finger tighten the fitting.

3 Turn the outlet fitting an additional 1/16 turn using pliers.

4 Route the other end of the tubing through the grommet on the lower left side of the detector enclosure.


Replacing Parts

Replace the Inlet Tubing

Refer to the instructions in this section when replacing the inlet tubing (INLET TUBING, PREP, SS, QUARTZ, 118, part number 10044121).

1 Remove the inlet tubing clamp and set it aside.

2 Unscrew the male nut that connects the inlet tubing to the flow cell assembly.

3 Remove the inlet tubing. To do this, hold the flow cell assembly in one hand. Grasp the shorter segment of tubing leading away from the flow cell assembly. Then gently twist the tubing to the left to unseat the circular portion from the groove around the flow cell assembly. Once the circular portion is free, pull down on the tubing to remove the segment from inside the flow cell assembly.

4 Insert the inlet tubing into the bottom of the flow cell assembly. Position the circular segment of tubing over the flow cell and seat it into the groove around the flow cell assembly.

5 Make sure the inlet tubing is seated in the flow cell assembly. Finger tighten the fitting.

6 Press together the two segments of inlet tubing as shown in the diagram below. The segment leading away from the flow cell should be almost in front of the segment leading into the flow cell.

7 Insert the inlet tubing clamp into its socket and tighten.

8 Route the other end of the tubing through the slot on the right side of the system.

Press together these two segments

inlet tubing clamp


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1 Use a syringe to initiate flow through the cell.

2 Check carefully along the sides of the cell for beads of liquid. A flashlight may be helpful.

3 If leakage is detected visually or if the area around the fitting feels cool to the touch, remove and clean the flow cell as well as its chamber. Then re-insert the flow cell and re-tighten the fittings.

Install the Flow Cell Assembly

1 Insert the flow cell assembly into the detector and tighten the flow cell assembly screw.



Visible Lamp

The detector contains a tungsten/halogen lamp for monitoring visible wavelengths. The visible lamp has a 500-hour warranted life.

Never touch a hot lamp. After turning off the system, always wait 30 minutes or more before replacing a lamp.

Refer to the instructions in this section when replacing the visible lamp (LAMP ASSY, HALOGEN, 118/119, part number 100325).







Replacing Parts

Remove the Visible Lamp


2 Locate the visible lamp and check the lamp connection.

If the lamp was not connected, you may want to reconnect and start the system to determine if this caused the problem.

3 Disconnect the lamp connector. Notice the orientation of the connector as you disconnect.

4 Grasp the visible lamp and turn it until the flat edge faces upward. Usually, this is just a slight rotation of the lamp in either direction.

5 Remove the lamp by pulling it away from the lamp housing.

Install the Replacement Visible Lamp

1 With the flat edge of the replacement lamp facing upward, insert the lamp into the lamp housing.

2 Once the lamp is inserted, rotate it slightly in either direction. Then gently pull on the lamp to ensure that it is secure.

3 Reconnect the visible lamp connector.



visible lamp

lamp housing

visible lamp connector


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rts Check for Stable Baseline

1 Connect the power cord to the rear panel receptacle and then to the power outlet.

2 Turn on the MAINS (power) switch on the rear panel. The power indicator light changes to green, the detector lamps turn on and begin to warm up, the fraction collection probe homes, and the software starts.

3 Allow the lamps to warm up. The lamps require a 15–30 minute warm-up period to achieve a stable baseline for use at high sensitivities.

4 In the PLC 2020 software, use Manual Control ( Chart) to confirm a stable baseline with the new lamp.

If the lamp fails again, there may be an internal alignment, a power supply, or a starting circuitry problem. Contact your Gilson-authorized representative; see Before Calling Us on page 6-12.

Reset the Maintenance Information

After the visible lamp is installed, and only at that time, reset the VIS Lamp (hours) Counter and Last Service Date in the PLC 2020 software.

1 On the Main Menu, tap Maintenance.

2 Under Detector and next to VIS Lamp (hours), tap Reset.

UV Lamp

The detector contains a deuterium lamp for monitoring UV wavelengths. The UV lamp has a warranted life of 750 hours or 180 days.

Always wear protective eyewear when looking at a UV-radiating lamp. Prescription glasses are acceptable, if they have Plasticon lenses. Plastic safety goggles with a UV filter provide maximum protection.

Never touch a hot lamp. After turning off the system, always wait 30 minutes or more before replacing a lamp.

Refer to the instructions in this section when replacing the UV lamp (LAMP ASSY, UV, 118 UV/119 UV, part number 100326).


Replacing Parts






Remove the UV Lamp


2 Use the 3 mm Allen wrench included with the Unpacking Instructions to remove the two screws that secure the detector to the system.

3 Pull out the detector to access the lamp housing.

Pull the detector out only until the middle set of rubber feet are resting on the edge of the enclosure.

4 Locate the UV lamp and check the lamp connection.

If the lamp was not connected, you may want to reconnect and start the system to determine if this caused the problem.

5 Disconnect the lamp connector. Notice the orientation of the connector as you disconnect.

6 Loosen the three thumbscrews that anchor the UV lamp to the top of the lamp housing.

7 Grasp the UV lamp by its base and remove the lamp from the lamp housing.

Always handle the UV lamp by the base, avoiding contact with its glass window. A hot lamp can cause a severe burn, and fingerprints can etch the window. To clean a lamp, use a soft cloth moistened with alcohol.

UV lamplamp

housing UV lamp connector

screws


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rts Install the Replacement UV Lamp

1 Make sure the replacement lamp is free of fingerprints and other blemishes.

2 Install the replacement lamp and tighten the thumbscrews.

3 Reconnect the UV lamp connector.



Check for Stable Baseline



3 Allow the lamps to warm up. The lamps require a 15–30 minute warm-up period to achieve a stable baseline for use at high sensitivities.

4 In the PLC 2020 software, use Manual Control ( Chart) to confirm a stable baseline with the new lamp.

If the lamp fails again, there may be an internal alignment, a power supply, or a starting circuitry problem. Contact your Gilson-authorized representative; see Before Calling Us on page 6-12.


After the UV lamp is installed, and only at that time, reset the UV Lamp (hours) Counter and Last Service Date in the PLC 2020 software.


2 Under Detector and next to UV Lamp (hours), tap Reset.


Replacing Parts

Pumping System

The table below gives an indication of replacement periods for maintenance parts, according to the type of use, intensive, regular, or occasional. The data assumes that the pump is working at half of its maximum flow rate and pressure. The nature of the liquid and the pump head model have only a small influence on these figures.

Replacement periods for pump head maintenance parts:

Parts Use Intensive (168 h/week)

Regular (40 h/week)

Occasional 10 h/week

Piston seal 2–3 months 1300–2000 h

6–9 months 1000–1400 h

1 year 500 h

Set of check valves 3–6 months 2000–4000 h

1 year 2000 h

2 years 1000 h

Piston assembly 6–12 months 4000–8000 h

2–3 years 2000–3000 h

5 years 2500 h

Anti-extrusion gasket 6-12 months 4000–8000 h

2–3 years 2000–3000 h

5 years 2500 h

Return spring 1 year 8000 h

2–3 years 2000–3000 h

5 years 2500 h


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rts Pump Heads

Refer to the instructions in this section when replacing the pump heads. The table contains part numbers for replacement pump heads.

Part Number Description360522 PUMP HEAD, 25SC, 25 ML/MIN, 4060 PSI360524 PUMP HEAD, 50SC, 50 ML/MIN 2030 PSI360530 PUMP HEAD,100SC, .50-100ML/MIN, 1015 PSI3645292 CLAMP, SC PUMP HEAD400956161 M6 X 16MM KNURLED T KNOB SCREW

Remove the Pump Head

1 Disconnect the inlet and outlet tubing from the pump head.

2 Loosen the thumbscrew holding the clamp on the pump head in place.

3 Turn the clamp counter-clockwise to remove it from the slots on both sides of the pump head.

Install the Pump Head

1 Orient the replacement pump head so the notch is at the bottom and then place it over the pin on the bottom of the pump.




After the piston seal has been replaced it is recommended to condition the pump head. Contact your Gilson-authorized representative for the PLC 2020 Personal Purification System conditioning procedure.

pin


Replacing Parts

Check Valves

The check valves are supplied in clean, pre-mounted cartridges. Each one is strictly checked and then packed separately.

Reliable flow rates will be achieved only if the check valves are kept in good operating condition by proper care and maintenance.

The check valve cartridge must be replaced if there is a check valve problem. This can be determined by an abnormal loss in flow rate.

The check valves must not be disassembled into sub-components. No check valve sub-component is available from Gilson.

Refer to the instructions in this section when replacing the inlet and outlet check valves. The table contains part numbers for replacement check valves.

Part Number Description3650170 INLET CHECK VALVE, 5/10/25/H1 HEAD3650180 OUTLET CHECK VALVE, 5/10/25/H1/H2 HEAD3650263 INLET CHECK VALVE CARTRIDGE, 50SC/100SC3650267 OUTLET CHECK VALVE CARTRIDGE,50SC/100SC

The check valve support screws can also be replaced while the check valves are removed. The table contains part numbers for replacement check valve support screws.

Part Number Description3645309 OUTLET VALVE SCREW, 5/10/25 PUMP HEAD3645310 INLET VALVE SCREW, 5/10/25 PUMP HEAD3645219 SCREW, OUTLET VALVE, 50/1003645356 INLET VALVE SCREW, 50/100 PUMP HEAD






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rts Replace the Check Valves

1 Unscrew the check valve support screws which contain the check valve cartridges.

2 Take out the check valve cartridges (if necessary, by means of a clean, compressed air flow).

3 Install the new check valve cartridges.

The check valve cartridges or their support screws are not interchangeable from inlet to outlet.

4 Connect the check valve support screws to the pump head.

Tighten the support screws at the nominal torque of 7 N.m which ensures tightness up to the maximum pressure. In the absence of a dynamometric wrench, tighten up to the mechanical stop, (i.e., to the metal on metal contact).






5 Run the pump and check for leaks around the support screws. Tighten any leaking support screw until the leakage stops.


After the check valves have been replaced, and only at that time, reset the Check Valve Last Service Date in the PLC 2020 software.


2 Under Pump A or Pump B and next to Check Valve, tap Reset.

inlet check valve

cartridge

outlet check valve

cartridge

inlet check valve support screw

outlet check valve support screw

25SC

25SC

50SC 100SC

50SC 100SC

pin


Replacing Parts

Piston Seal

The piston seal consists of a seal ring made of either graphite reinforced PTFE (black) or HDPE (yellow) and a spring made of Hastelloy® C-276 (wire) or Elgiloy® HT (ribbon). It must be changed whenever a piston seal leak occurs. This can be easily detected by the appearance of liquid at the notch located at the bottom of the pump head.

Choose piston seals based on the solvents used:

• Use the black PTFE piston seals with organic solvents

• Use the yellow HDPE piston seals with aqueous solvents

The table contains part numbers for replacement piston seals.

Part Number Description5463050025 SEAL, PISTON, PTFE, BLACK, 25SC/25WTI5463050825 SEAL, PISTON, HDPE, YELLOW, 25SC5463070020 SEAL, PISTON, PTFE, BLACK, 50SC5463070820 SEAL, PISTON, HDPE, YELLOW, 50SC5463394062 SEAL, PISTON, PTFE, BLACK, 100SC5463394862 SEAL, PISTON, HDPE, YELLOW, 100SC

Due to fatigue of the steel, the return spring should be replaced with every five piston seal replacements. The table contains part numbers for replacement return springs.

Part Number Description36300058 RETURN SPRING 5/10/25SC36300062 RETURN SPRING, 50/100SC

The following parts of the pump head can also be replaced while the pump head is disassembled: anti-extrusion gasket, spring spacer, piston tail guide, and ball housing assembly. The table contains replacement part numbers.

Part Number Description3650016K ANTI EXTR. GASKET, KEL-F, 25 SC3650017K ANTI EXTR. GASKET, KEL-F, 50 SC3650018K ANTI EXTR. GASKET, KEL-F 100 SC3650019 SPRING SPACER, 5,10,25 SC3650020 SPRING SPACER FOR 50 & 100 SC HEADS3650027 PISTON TAIL GUIDE 5,10,25 SC3650028 PISTON TAIL GUIDE 50 & 100 SC3650067 BALL HOUSING ASSY, 5,10,25 ML3650068 BALL HOUSING ASSY. 50 & 100 ML HEADS


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rts Remove the Pump Head




Disassemble the Pump Head

1 Unscrew the threaded cover on the pump head.

2 Take out the piston tail guide, the piston assembly, the spring spacer, and the return spring.

3 Loosen the two screws on the back of the pump head and remove the ball housing and the housing spacer.

4 Remove the anti extrusion gasket.

5 Carefully take out the piston seal.

If the piston seal remains tightly seated in the head body, pry it out with care in order to avoid damaging the pump head body.

piston sealanti-extrusion gasket

housing spacerreturn spring

spring spacerthreaded cover

screws

head body

ball housing assemblypiston assembly

piston tail guide


Replacing Parts

Install the Piston Seal

Inspect the piston and the area around the piston seal to ensure that they are clean, undamaged and completely free of any particles.

Use the piston seal mounting tool (composed of a seal pusher and a seal guide) to install the new piston seal.

1 Set the guide onto the pump head body with its bevelled edge out.

2 Insert the new piston seal in the guide with its spring side in.

3 Press the seal pusher with your finger until the piston seal goes down to the pump head.

Reassemble the Pump Head

1 Place the anti extrusion gasket over the piston seal.

2 Line up the ball housing assembly, the housing spacer, and the pump head body with the matching hole or notch on the same side. Reinstall the two screws on the back of the pump head body.

Place the pump head body on a flat surface to facilitate the mounting of other parts.

3 Reinstall or replace the return spring.

Note: Due to fatigue of the steel, the return spring should be replaced with every five piston seal replacements.

4 Reinstall the spring spacer, the piston assembly, and the piston tail guide.

Ensure that the piston assembly and the spring spacer are correctly assembled (spherical male into spherical female, refer to the diagram for more information).

5 Reinstall the threaded cover on the pump head.

piston seal mounting toolseal guide

seal pusher

head body

bevelled edge outspring side in

piston seal


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rts Install the Pump Head





After the piston seal has been replaced it is recommended to condition the pump head. Contact your Gilson-authorized representative for the PLC 2020 Personal Purification System conditioning procedure.


After the piston seal has been replaced, and only at that time, reset the Piston Seal Last Service Date in the PLC 2020 software.


2 Under Pump A or Pump B and next to Piston Seal, tap Reset.

Piston Assembly

Refer to the instructions in this section when replacing the piston assembly. The table contains part numbers for replacement piston assemblies.

Part Number Description3650011 PISTON FOR 25 SC PUMP HEAD3650012 PISTON FOR 50 SC PUMP HEAD3650013 PISTON FOR 100 SC PUMP HEAD





pin


Replacing Parts

Replace the Piston Assembly

1 Unscrew the threaded cover on the pump head.

2 Take out the piston tail guide and the piston assembly.

3 Install the new piston assembly.

4 Reinstall the piston tail guide.

5 Reinstall the threaded cover on the pump head







After the piston assembly has been replaced, and only at that time, reset the Piston Assembly Last Service Date in the PLC 2020 software.


2 Under Pump A or Pump B and next to Piston Assembly, tap Reset.

return springspring spacer

threaded cover

ball housing assemblypiston assembly

piston tail guide

pin


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rts Inlet Filter

To protect the check valves on the pump head, an inlet filter must be used with all solvents. Inlet filters must be in good condition for the pump to operate efficiently.

Change the inlet filter (INLET FILTER,20UM 5/16-24 FOR 3/16 TBG, part number 4957232) if you suspect that it has become plugged.

In-Line Filter

The in-line filter protects the injection valve and the column. A plugged in-line filter may cause pressure buildup and leaks.

Routinely check and clean the interior of the filter, and when necessary replace the filter cartridge.

The table contains part numbers for replacement parts.

Part Number Description36007004 FLTR, IN-LINE PREP 10MICRON SS36007005 FLTR CRTG, IN-LINE 10 MICRON FOR 36007004

Refer to the documentation included with the filter or cartridge for replacement instructions.


After the in-line filter has been replaced, and only at that time, reset the Inline Filter Last Service Date in the PLC 2020 software.


2 Next to Inline Filter, tap Reset.


Replacing Parts

Fraction Collector

Fraction Collection Probe

Refer to the instructions in this section when replacing the fraction collection probe. The table contains part numbers for replacement probes and fittings.

Part Number Description21037017 TUBE,DISP .030 IDX.063ODX2.440 PLC49041011 FERRULE, 1/16", RED (P-200R)49041021 NUT,HEADLESS,1/4-28, 1/16 TUBING(P-286)21037019 TUBE,DISP 0.080IDX0.124ODX2.440 PLC49041015 FERRULE, FLANGELESS, 1/8", TEFZEL(P-300)49041054 NUT,HEADLESS 1/4-28,1/8"TUBING(P-386)



3 Remove the original fraction collection probe and fittings and replace with the new ones.



Fraction Collector Valve Assembly

Refer to the instructions in this section when replacing the fraction collector valve assembly (FC VLV AND TBG REPL. KIT, PLC, part number 21037029).







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rts Remove the Fraction Collector Valve Assembly

1 Disconnect the following:

a) Valve cable from the system

b) FC valve to waste (disconnect at drain)

c) FC valve to detector (Open the panel door to access and remove the PEEK tubing from the coupling. The black grommet may need to be removed to get the fitting through.)



4 Remove the tubing assembly from the IGUS cable.

Replace the Fraction Collector Valve Assembly

1 Connect the fraction collection probe to the replacement valve.



4 Place the tubing assembly in the IGUS cable.

5 Connect the following:

a) Valve cable to the system

b) FC valve to waste (connect at drain)

c) FC valve to detector

Power on the PLC 2020



disconnect


Replacing Parts


After the fraction collector valve has been replaced, and only at that time, reset the Fraction Collector Valve Last Service Date in the PLC 2020 software.


2 Under Fraction Collector and next to Piston Assembly, tap Reset.


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rts Injection Valve

Injection Valve

Refer to the instructions in this section when replacing the injection valve (VALVE,INJ .030ID 2POS 6PORT SS 1/16, part number 49400009).

Remove the Valve

1 Under Manual Control in the PLC 2020 software, tap Inject to switch the valve to the inject position.

2 Remove the injection port and disconnect all plumbing connections to the valve (including ports 2–6 and the weep hole).

3 Use a 9/64" Allen wrench to loosen the screw on the valve collar and then remove the valve.

Install the Valve

1 Ensure that the notches in the coupler are aligned vertically with the screws in the valve collar.

2 Orient the replacement valve with port 1 at the top and install the valve on the system.

3 Use the 9/64” Allen wrench to tighten the screw on the valve collar.


After the injection valve has been replaced, reset the Injection Valve Switches Counter and Last Service Date in the PLC 2020 software.


2 Under Manual Injector and next to Injection Valve Switches, tap Reset.

screw

notch


Replacing Parts

Injection Port

Refer to the instructions in this section when replacing the injection port (PORT,MNL INJ VLV .75 20GA SST, part number 49400007).

1 Remove the installed injection port.

2 Insert a needle into the new injection port.





After the injection port has been replaced, reset the Injection Port Last Service Date in the PLC 2020 software.


2 Under Manual Injector and next to Injection Port, tap Reset.

Injection Port Liner

Refer to the instructions in this section when replacing the injection port liner (PORT,MNL INJ VLV .75 20GA ETFE LINER KIT, part number 49400008).

1 Remove the installed injection port.

2 Remove the liner from the injection port and replace with a new one.

3 Insert a needle into the injection port.





After the injection port liner has been replaced, reset the Injection Port Last Service Date in the PLC 2020 software.


2 Under Manual Injector and next to Injection Port, tap Reset.


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rts Injection Valve Rotor

Refer to the instructions in this section when replacing the injection valve rotor (ROTOR,VALCON H,6 PORT,C2-30R6, part number 494C230R6).

Remove the Valve

1 Under Manual Control in the PLC 2020 software, tap Inject to switch the valve to the inject position.

2 Remove the injection port and disconnect all plumbing connections to the valve (including ports 2–6 and the weep hole).

3 Use a 9/64" Allen wrench to loosen the screw on the valve collar and then remove the valve.

Disassemble the Valve

1 Use a 9/64" Allen wrench to remove the socket head screws which secure the cap (stator) on the valve.

2 To ensure that the sealing surface of the cap (stator) is not damaged, rest it on its outer face. Or, if the tubing is still connected, leave it suspended by the tubing.

3 With your fingers or a small tool, gently pry the rotor away from the driver.

4 Examine the rotor sealing surface for scratches. If scratches are visible to the naked eye, replace the rotor. If no scratches are visible, clean all the parts thoroughly with an appropriate solvent, taking care that no surfaces get scratched. (A common problem is the formation of buffer crystals, which are usually water-soluble.) It is not necessary to dry the rotor.

Reassemble the Valve

1 Replace the rotor in the driver, making sure that the rotor sealing surface with its engraved flow passages is facing out. The rotor is asymmetrical to prevent improper orientation.

2 Replace the cap (stator). Insert the socket head screws and tighten them gently until snug. Do not over-tighten them— the screws simply hold the assembly together and do not affect the sealing force, which is automatically set as the screws close the cap against the valve body.

screw

driver

rotor

valve body

cap

socket head screws (9/64")


Replacing Parts

Install the Valve

1 Ensure that the notches in the coupler are aligned vertically with the screws in the valve collar.

2 Orient the replacement valve with port 1 at the top and install the valve on the system.

3 Use the 9/64" Allen wrench to tighten the screw on the valve collar.

4 Test the valve by pressurizing the system. If it doesn’t hold pressure, contact your Gilson-authorized representative.

notch


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rts Fuse

Refer to the instructions in this section when replacing the fuse. The table contains part numbers for replacement fuses.

Part Number Description6730404007 4 AMP T-4 SLO-BLO FUSE 250V6770100411 FUSE DRAWER, 4303.2401, 220V





4 Locate the fuse drawer on the rear panel.

5 Insert a small screwdriver into the notch under the fuse drawer.

6 Using the screwdriver, lift up to remove the fuse drawer. The fuse drawer contains two 4.0A, “T”-type fuses.

7 Remove the old fuses and insert the new fuses.

8 Insert the fuse drawer into its receptacle.

A blown fuse may indicate the existence of another problem in the system. If the replacement fuses blow, don’t try others. Contact your Gilson-authorized representative. Refer to Before Calling Us on page 6-12.

Additional Replacement Parts

Part Number Description21031062 LCTR, (3) 33X-SERIES RACKS PLC49081702 ADAPTER, BARBED,1/8MPT TO 3/8 TUBE,HDPE21031063 BRKT, COL HLDR PLC21037026 RTNR,SLVT BTL PLC21037027 BRKT, WASTE BTL PLC


Chapter 6

Contents

Error Codes and Messages ...................................................... 6-2

Troubleshooting ......................................................................... 6-4

Repair and Return Policies ....................................................6-12

Troubleshooting


Trou

bles

hoot

ing Error Codes and Messages

When an error occurs during a run, the Method stops, the pumps stop, an error message appears, and the run light flashes.

Obtain Error Code and Message

When a user-defined error occurs, details about the error appear on screen. To obtain the current error code and message for other errors, do the following:

1 On the Main Menu, tap Utilities.

2 Select the Tools tab and then, tap Gilson Ethernet Utility.

3 Select PLC 2020 from the list and then tap Connect.

4 Select Get Error from the command drop-down and then tap Go. The Results will display the current error.

Note: If the system returns error 99 (Accessory Error), it is likely a detector error. Repeat step 3, but select the detector and then tap Connect. Repeat step 4 to obtain the detector error code.

5 For assistance with resolving the error, contact your Gilson-authorized representative.

Refer to the following tables for a list of error codes and messages.

PLC 2020 Error CodesError Error Text

0 No Error

10 Unknown Command

11 Invalid NV-RAM Address

13 Bad Parameter Entered

14 FIFO Full

15 FIFO Add

16 Character Limit

17 X Axis Park Location

18 Y Axis Park Location

20 X Axis Unhomed

21 Y Axis Unhomed

23 I Axis Unhomed


Troubleshooting

24 X Axis Moving

25 Y Axis Moving

27 I Axis Moving

28 X Axis Stall

29 Y Axis Stall

32 X Axis Encoder

33 Y Axis Encoder

36 X Axis Speed Range

37 Y Axis Speed Range

40 X Axis Target Range

41 Y Axis Target Range

99 Accessory Error

Detector Error Codes

These error codes are specific to the detector.

Error Error Text

00000000 Detector is functioning properly

00000001 Digital Signal Processor malfunctioning

00000002 Invalid age for UV lamp

00000004 UV lamp too old (Beyond lamp alarm setting)

00000008 Invalid age for VIS lamp

00000010 VIS lamp too old (Beyond lamp alarm setting)

00000020 Invalid UV lamp identification

00000040 Monochromator not properly aligned (calibration peak too close to stop), or UV lamp did not start

00000080 Monochromator not properly aligned (calibration peak too far from stop), or UV lamp did not start

00000100 Too much stray light on low wavelength side of calibration peak, or UV lamp did not start

00000200 Too much stray light on high wavelength side of calibration peak, or UV lamp did not start


Trou

bles

hoot

ing Troubleshooting

This section provides possible solutions when troubleshooting the PLC 2020 Personal Purification System. If these solutions are ineffective, contact your Gilson-authorized representative.

System Not Operational

• Make sure the power is turned on.

• Check AC power cord connections.

• Try a different AC outlet.

• Check fuses; replace, if necessary. Refer to the instructions for replacing the Fuse on page 5-32.

System Blows Fuses

Contact your Gilson-authorized representative.

Run Light Blinking

An error has occurred; refer to Error Codes and Messages on page 6-2.

Input Contacts Not Operational

• Make sure connections into the terminal block connector are secure.

• Make sure the terminal block connector is secure in the input/output port.

• Check the connections for proper pin assignments.

• Be sure pins from external devices are assigned correctly.

• Check polarity of input. Inputs should be a contact closure. If not, it must be TTL level (logic 0 activates).

• Confirm that the source supplying input to the system is working.

Output Contacts Not Operational

• Make sure connections into the terminal block connector are secure.

• Make sure terminal block connector is secure in the input/output port.

• Check the connections for proper pin assignments.

• Output from the system should be compatible with device to which it is interfaced. Outputs are contact closures.


Troubleshooting

Detector Troubleshooting

Baseline Noise

• Allow lamps to warm up for 15–30 minutes.

• Eliminate drafty air currents, smoke, and variations in ambient temperature. Move the system away from windows or doors.

• Degas solvents.

• Check fittings for a leak in the system; tighten or replace, if necessary.

• If you see baseline deflection when back pressure is exerted on the flow cell you probably have bubbles trapped in the cell. Try one of these methods to eliminate bubbles:

• Increase back pressure on the cell by connecting pressure regulator tubing to the flow cell outlet.

• Increase back pressure by raising flow rate and using a syringe or septum to momentarily block exit stream.

• Flush flow cell using a syringe filled with fresh solvent.

• A lamp may be weak; check lamp hours counter in the PLC 2020 software (Maintenance - Device tab) for possible warranty replacement or preventative maintenance due. Refer to the instructions for replacing the Visible Lamp on page 5-10 and/or the UV Lamp on page 5-12.

• Check flow cell for contamination or damage; clean or replace, if necessary.

Baseline Spikes

• Degas solvents.

• Press the POWER button on the front of the system to turn off the lamps. Optionally, save any unsaved information. Visually inspect flow cell for air bubbles.


• Reduce pump noise by changing the compressibility setting in the PLC 2020 software and by examining check valves.

• Check the solvent lines for air; prime, if necessary.


Trou

bles

hoot

ing A Lamp Fails to Ignite/A Lamp Fails While On

A lamp connector may be loose or a lamp may require replacement. Check lamp hours counter in the PLC 2020 software (Maintenance - Device tab) for possible warranty replacement or preventative maintenance due. Refer to the instructions for replacing the Visible Lamp on page 5-10 and/or the UV Lamp on page 5-12.

Baseline Drift

• Allow lamps to warm up for 15–30 minutes.

• Eliminate changes in ambient temperature. If drift is dependent on temperature, eliminate drafts and avoid areas of direct sunlight.

• Check for contaminated or “bleeding” column. If drift disappears after removing column connections, regenerate or replace column.

• Degas solvents.


• If you see baseline deflection when back pressure is exerted on the flow cell you probably have bubbles trapped in the cell. Try one of these methods to eliminate bubbles:




• Check each pump to determine that flow rate is constant.


• Check flow cell for leak or contamination; clean or replace, if necessary.

• Check purity of solvents.


Troubleshooting

Autozero Out of Range

Autozero out of range is a result of high background absorbance. The detector cannot reduce the baseline to zero, but will reduce the baseline as much as possible. Operation continues.

• Flow cell may be coated with highly absorbing eluents; refer to Cleaning the Inside of the Flow Cell on page 4-6 for flushing instructions.

• Select a new solvent or monitor wavelength; refer to Mobile Phase Tips on page 7-5.

• Check purity of solvents.

Out of Range

• Check purity and UV/VIS-cutoff of solvents; use HPLC-grade solvents.

• Degas solvents.

• There may be bubbles in the flow cell. Try one of the following:




• Check for contaminated or “bleeding” column; regenerate or replace column, if necessary.


• A lamp connector may be loose or a lamp may be weak; check lamp hours counter in the PLC 2020 software (Maintenance - Device tab) for possible warranty replacement or preventative maintenance due. Refer to the instructions for replacing the Visible Lamp on page 5-10 and/or the UV Lamp on page 5-12.

Peaks Too Broad

• Check the peak width setting in the Detector Settings task in the PLC 2020 software—it may be set too high. Measure the narrowest peak; then set new value.

• Column age may cause loss of resolution and larger peak widths; replace or regenerate column, if necessary.


Trou

bles

hoot

ing Decreased Response to Known Sample


• Verify sample concentration and mobile phase solvent.


• Check wavelength and sensitivity values in the Detector Settings task in the PLC 2020 software. Sensitivity values may be set too high; reduce, if necessary.

Abnormally High System Pressure

• Check for blockage in flow cell or tubing.

• Check for blockage in the column; replace column, if necessary.

Hydraulic (Pump) Problems

Leaks from the Pump Head

The piston seal may be defective. Replace the piston seal. Refer to the instructions for replacing the Piston Seal on page 5-19.

Abnormally Low Flow Rate

• Check all tubing and connections for leaks.

• The inlet filter may be plugged. Clean or replace the inlet filter. Refer to the instructions for replacing the Inlet Filter on page 5-24.

• Check that solvent lines are primed.

• A check valve may be defective. Clean or replace the check valve. Refer to the instructions for replacing the Check Valves on page 5-17.

• Check that the pump heads are mounted properly.

• Place solvent bottles higher than the pump heads.


Troubleshooting

Air Bubbles Appear in Solvent Inlet Tubing

• Check all low pressure connections.

• The inlet tubing may be loose; tighten the connection (but do not over tighten).

• The nut and/or the ferrule may be damaged; replace the nut and/or the ferrule.

• An inlet filter may be clogged; clean or replace the inlet filter.

Air Bubbles Appear Only in Outlet Tubing

The outlet tubing may be loose; tighten the connection (but do not over tighten).

Abnormally High Pressure

• The outlet filter may be plugged; clean or replace the outlet filter.

• The column particle size may be too small or the column may be plugged; change or flush column.

Dampener

Poor Dampening Effect

• The pressure may be too low to activate the dampener effect; increase the pressure using a capillary tubing or increase the flow rate.

• The membrane in the dampener may be defective; contact your Gilson-authorized representative.

Loss of Dampening Effect during Operation

The membrane in the dampener may have failed; contact your Gilson-authorized representative.

Blue-tinted Fluid from Column

The membrane in the dampener may have broken; contact your Gilson-authorized representative.


Trou

bles

hoot

ing Fraction Collector

Unit Makes Loud Noise When Changing X or Y Position in Run

Check for obstruction to X or Y movement. Press the POWER button on the front of the system to relax the arm. Optionally, save any unsaved information. Slowly move the XY arm. It should move smoothly.

Fraction Fluid Missing the Tube

• Check to be sure rack codes in the PLC 2020 software match the racks in the system.

• Check for proper rack installation.

• Check that the fraction collection probe is not bent.

• Check mechanical XY arm assembly for alignment. Press the POWER button on the front of the system to relax the arm. Optionally, save any unsaved information. Wait 10 seconds and then press the POWER button on the front of the system again to restart the system and reset the alignment.

Separation Problems or Not Recognizing Peaks

• Check values for properties in the Fraction Collection Settings or Conditional Fraction Collection task in the PLC 2020 software.

• Bubbles in mobile phase creating spikes on chromatogram; degas solvents or eliminate plumbing leak that is causing bubbles.

• Check that the FC Delay Volume in the PLC 2020 software (Method Builder - Configuration tab) is set correctly.

• Check wavelength values in the Detector Settings task in the PLC 2020 software.

FC Valve Not Functioning or Functioning Incorrectly

Check the valve’s plumbing connections and electrical connection to the system.


Troubleshooting

Injection Valve

Mobile Phase Pressure is Steadily Increasing

Check that the injection valve tubing is not crimped or plugged.

Liquid Leaking from the Bottom of the Injection Valve

• Check that all fittings are secure in the valve; tighten, if necessary.

• Check that the stator is securely tightened to the valve; tighten, if necessary.

• Replace the injection port liner. Refer to the instructions for replacing the Injection Port Liner on page 5-29.

Bubbling Liquid from Waste Port or Injection Port

• Replace injection valve’s rotor. Refer to the instructions for replacing the Injection Valve Rotor on page 5-30.

• Check that the injection valve waste tubing is not crimped or plugged.

Liquid Leaking from Weep Hole

• Check if the injection valve waste tubing is clogged; replace, if necessary.

• Replace the injection port. Refer to the instructions for replacing the Injection Port on page 5-29.


Trou

bles

hoot

ing Repair and Return Policies

Before Calling Us

Gilson-authorized representatives will be able to serve you more efficiently if you have the following information:

• the serial number of the system. The serial number is located on the inside left panel (below the display)

• the installation procedure you used

• list of concise symptoms

• list of operating procedures and conditions you were using when the problem arose

• list of other devices connected to the system and a description of those connections

• list of other electrical connections in the room

Warranty Repair

Units covered under warranty will be repaired and returned to you at no charge. If you have any questions about applicability, please contact your local distributor.

Non-Warranty Repair

For out-of-warranty repairs, contact your local distributor. A Customer Service representative will discuss service options with you and can assist in making arrangements to return the equipment, if necessary.


Troubleshooting

Return Procedure

Contact your local distributor’s Customer Service Department to obtain authorization before returning any Gilson equipment. To return a piece of equipment:

• Carefully pack the unit to prevent damage in transit. Check with your distributor regarding proper method of shipment. No responsibility is assumed by Gilson or your distributor for damage caused by improperly packaged instruments. Indicate the authorization on the carton and on the packing slip.

• Always insure for the replacement value of the unit.

• Include a description of symptoms, your name, address, phone number, and purchase order to cover repair costs, return and shipping charges, if your institution requires it.

Unit End-of-Life

When a unit reaches the end of its useful life, refer to www.gilson.com for directions and information about the end-of-life policy. This is in accordance with the European Union Directive 2002/96/EC on Waste Electrical and Electronic Equipment (WEEE).

http://www.gilson.com


Chapter 7

Contents

Compressibility ........................................................................... 7-2

Solvent Miscibility Table .......................................................... 7-4

Mobile Phase Tips ....................................................................... 7-5

Reference


Refe

renc

e Compressibility

The following table provides the compressibility values for some common mobile phases.

Solvent Temperature (ºC) Compressibility (Mbar-1)

Water

20 4625 4630 4540 45

Methanol

20 121–12325 125–12730 129–13040 138

Acetonitrile 99Tetrahydrofuran 93

Benzene

20 94–9525 96–9730 101–10340 110

n-Hexane

20 150–16525 161–17130 165–18040 183

Carbon Tetrachloride

20 103–10525 106–10830 112–11340 120–122

Water-Methanol, 10-90 117Water-Methanol, 20-80 86Water-Methanol, 40-60 56Water-Methanol, 50-50 52Water-Methanol, 60-40 46Water-Methanol, 80-20 40

Chloroform

20 97–10125 9730 108–11040 118–119


Reference

Methylene Chloride 25 97

Ethanol

20 110–11225 114–11630 118–11940 126–127

Acetone

20 126–12725 12430 13340 144–156

n-Heptane

20 140–14525 142–14930 150–15540 160

Diethyl Ether25 195–20025 195–20030 208–209


Refe

renc

e Solvent Miscibility Table

When changing solvents, make sure that the new solvent is miscible with the previous one. Otherwise, use an intermediate solvent.

MISCIBLE

IMMISCIBLE

ACETIC ACIDACETONEACETONITRILEBUTYL ALCOHOLCHLOROFORM (1)

CYCLOHEXANEDICHLOROETHANE (1)

DICHLOROMETHANEDIMETHYLFORMAMIDEDIMETHYL SULFOXIDEDIOXAN (2)

ETHYLACETATEETHANOLDI-ETHYLETHERHEPTANEHEXANEMETHANOL (3)

METHYLETHYL KETONEI-OCTANEPENTANEISOPROPANOLDI-PROPYLETHERTETRAHYDROFURANTOLUENEWATERXYLENE


Reference

Mobile Phase Tips

Check UV and Visible Absorbance of Solvents

Remember to use only HPLC-grade solvents for your mobile phase. Impure solvents can absorb significant quantities of UV or visible light even when the cutoff may be below the monitoring wavelength. This can result in an inability to accurately set the baseline to zero.

Manufacturer’s specifications list the UV and visible cutoff for a solvent. This is the wavelength below which a solvent should not be used for UV or visible detection. The cutoff is the wavelength at which the solvent is 90% opaque (absorbance 3, 1.0 AUFS). Interference can still occur above the rated limit, particularly when the solvent composition changes during a run. This frequently appears as a gradual baseline shift.

The table below lists commonly used solvents with low UV and visible cutoffs.

Solvent UV/Vis Cutoff

Acetonitrile 190 nm

Cyclohexane 200 nm

Cyclopentane 200 nm

Decahydronaphthalene 200 nm

Ethanol 210 nm

Heptane 200 nm

Hexadecane 190 nm

Hexane 195 nm

Hexanes 210 nm

Isooctane 197 nm

Methanol 205 nm

Methylene chloride 233 nm

Pentane 205 nm

i-Pentanol 208 nm

i-Propanol 205 nm

n-Propanol 240 nm

Tetrahydrofuran 212 nm

Water <190 nm

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