CLIA CATEURINE SC

The UScreeMethamphetBarbiturates Antidepressa

The Uscreenoff levels. For HealthcaFor in vitro d

WHAT IS Th

The UScreendrugs listed WHAT IS TH

Marijuana Cocaine (CAmphetamMethamphMorphine (Morphine 2MethylenedBarbiturateBenzodiazMethadoneOxycodonePhencyclidTri-cyclic ABuprenorp

WARNINGS

• This• Disc• Kee

MATERIAL P•••••

STORAGE A• Stor• Kee• DO

PRINCIPLE UScreen2 DrIt is chromatdrug monocl When the temonoclonal below the deconjugate imstrip, that, re When sampdrug monocrespective ddistinct color To serve as performed p

SPECIMEN

WHEN TO CCollect urinedetecting anrefer to the package inse HOW TO CO1. Remov

the caaccept

2. Observsubstit

HOW TO PE

Test must be1. After th2. Read t3. Remov

your cu4. Remov5. Do not

EGORIZATION:REENING TEST

en2 Drugs of Abustamine (MET), Me

(BAR), Benzodiants (TCA) and Bu

n2 test cup is a rap

are professionals idiagnostic use only

he USCREEN2 DR

n2 Drugs of Abusein the table below.

HE CUT-OFF VALUDrug(Identi

(THC) COC) mine (AMP) hetamine (MET) (MOP) 2000 (OPI) dioxymethamphetes (BAR) epines (BZO) e (MTD) e (OXY) dine (PCP) Antidepressants (Thine(BUP)

S AND PRECAUTIs kit is for external card after first use.p out of the reach

PROVIDED Delet• 25 Test devices• Desiccant Pouc• One (1) Instruct• One (1) Adulter• 25 Security sea

AND STABILITY re at 39 ~ 86 ºF (4 p away from direcNOT FREEZE.

rugs of Abuse Cuptographic absorbelonal antibody (moest is activated, thantibody conjugatetection level of t

mmobilized in the Tegardless of its intele drug levels are

clonal antibody codrug-protein conjugred band in the tes

a procedure conroperly.

COLLECTION AN

COLLECT URINE e samples after miy drug of abuse. Esection “WHAT ISert, to determine thOLLECT URINE?ve a test cup from p from the test cutable to collect extve the temperaturtute urine, or liquid

ERFORM THE TES

e performed at roohe urine has been temperature immeve Cup label, and up is equipped witve the label and ret read results afte

UScreen

: WAIVED T RESULTS IN

se Cup tests for ththylenedioxymethaiazepines (BZO), uprenorphine(BUP

pid, screening test

including professioy.

RUGS OF ABUSE

e Cup is an immun. The test you pu

UE AND APPROXfier)

amine (MDMA)

CA)

ONSuse only The test cannot bof children

e “OF THE KIT” s, one test cup per ch - Remove desiction Sheet ation color compa

als (if Provided)

~ 30 ºC) in the set sunlight, moistur

p is a competitive imnt device in which

ouse) conjugate binhe urine is absorbte, and flows acrohe test, respectiveTest Region (T) of ensity, indicates a at or above the dnjugate, preventingate immobilized st region, indicatingtrol, a colored line

ND PREPARATION

FOR THE TEST?nimum detection t

Each drug is cleareS THE CUT-OFF he minimum/maxim

the foil pouch by tup, and void directra sample. re strip affixed on

d other than urine.

ST?

om temperature. 65collected, tighten

ediately to verify thverify that adulterah specimen validit

ead the results. Waer 5 minutes. Res

n2 Drugs

5 MINUTES

he possible use ofamphetamine - ec

Methadone (MTP).

for the qualitative

onals at point of ca

CUP?

no-chromatographiurchased may test

XIMATE DETECTICut-off l

50 ng/mL 300 ng/mL1000 ng/m1000 ng/m300 ng/mL2000 ng/m500 ng/mL300 ng/mL300 ng/mL300 ng/mL100 ng/mL25 ng/mL 1000 ng/m10 ng/mL

be used more than

pouch. Each poccant from cup. The

rison chart for inte

aled pouch up to te and heat.

mmunoassay that , drugs within a urnding sites. bed into each testoss a pre-coated me drug monoclonathe test strip. Thisnegative test resuetection level of th

ng the respective in the Test Regiog a preliminary pose will appear at th

N

ime following susped by the body and

VALUE AND APPmum detection tim

tearing at the notctly into the test cu

n the test cup, imThe temperature r

5 - 86ºF (18 - 30ºClid, and place the at urine temperatuant pad colors arety/adulterant pads.ait 5 minutes to desults after more tha

of Abuse C

f marijuana (THC)stasy (MDMA), Mo

TD), Phencyclidine

e detection of multi

are sites.

c assay for the qufor any combinatio

ON TIME? level Minimu

2 hoursL 1-4 houmL 2-7 houmL 2-7 houL 2 hoursmL 2 hoursL 2-7 houL 2-4 houL 2-7 houL 3-8 houL 1-3 hou

4-6 houmL 8-12hou

4 hours

• Do not once • Do not

• Do not

uch contains a tese desiccant is for s

erpretation of adult

the expiration date

is used to screen frine sample, comp

t strip by capillarymembrane. When al antibody conjugs produces a colorlt.

he test, the free drdrug monoclonal

on (T) of the devisitive result. he Control Region

pected drug use. d is detected in thePROXIMATE DET

mes, and cut-off lev

ch. Use it as soon p. Instruct the don

mmediately, to detrange from 32°C to

C). test cup on a flat s

ure is within the ac within acceptable.)

etermine a positivean 5 minutes may

Cup

), Cocaine (COC),orphine 300 (MOPe (PCP), Oxycod

iple drugs in huma

ualitative determinaon of drugs listed

um detection time

s urs urs urs s s urs urs urs urs urs urs urs

s

use if pouch is punuse test kit beyondread results after 5

st cup with integratstorage purposes

teration test results

e.

for the presence opetitively combined

y action, mixes wdrug within the u

gate binds to the red Test line in the

rug in the sample antibody conjuga

ce. This prevents

n (C), of each stri

Urine collection te urine at different tTECTION TIMES?vel for each drug.

as possible. Instrunor to fill the cup

termine if the urino 38°C (90 ºF-100

surface. cceptable range. 90e range according

result.be not accurate a

, Amphetamine (AP), Morphine 2000 done, (OXY), Tri-

an urine at specifie

ation of the presenin the table below.

Maximum detectiontime

Up to 5+ days 2-4 days 2-4 days 2-4 days 2-3 days 2-3 days 2-4 days 1-3 weeks1-4 days 1-3 days 1-2 days 7-14days 2-7 days 1-3 days

nctured or not sead expiration date 5 minutes

ted test card only.

s (if equipped)

f various drugs in ud to a limited numb

ith the respective rine sample is a lrespective drug-pre Test Region (T) o

binds to the respeate from binding t the development

ip, if the test has

ime is very importtimes and rates. P?” contained within

uct the donor to reto the 30 mL mar

ne is diluted by w0ºF) is acceptable.

0 – 100oF (32 – 38to adulteration gui

and should not be r

AMP), (OPI), cyclic

ed cut

nce of .

n

aled

urine. ber of

drug levels rotein of the

ective to the t of a

been

ant in lease n this

move rk. It’s

water,

8oC) ide (If

read.

READINPrelimiA rose-ppositive NegativIf a rosecorrespoInvalid If a coloAnotherdistribut Note: T

CertainREGIONIMPORTbe usechromatjudgmenWhat IsThe defAbuse Cdrugs an What IsThe defDiluted

TEST L1. T

d2. A

o3. T

le

ASSISTIf you h SUMMAAmphedoses inanxiety, as unchin the bamphetaBarbituthat is sDependbarbiturbarbiturBenzodof anxieas sedadependeare takebenzodiCocainepsycholpsycholexcreted8 hourscocaineMethyleadverseanxiety, 3,4-met3,4-metmetabolMethadThe psymethadohas a biOpiatesopiates depressmetabolmorphinheroin, mOxycodand sedmetaboloxycodoPhencyused asintravensense oin urine Tetrahysmokedloss of heart ra

NG THE RESULTSnary positive (+)pink band is visiblresult is indicated

ve (-) e-pink band is visibonding drug of tha

or band is not visibr test should opentor from whom you

There is no meanin

lines may appeN, NO MATTER HTANT: This assay

ed in order to tography/mass spnt should be applies A False Positivefinition of a false pCup. The most cond nutritional supp

s A False Negativefinition of a false nor adulterated urin

LIMITATIONS This test has beendevice to test subsAdulterated urine soxidize drug analytThis test is a qualitevel of intoxication

TANCE have any questioARYtamine/Methampnduce euphoria, aparanoia, psycho

hanged drug alongody. As a result, uamine metabolite.

urates (BAR) are similar to alcohol iding on the dose, frates can occur. Brate drug class typdiazepines (BZO)ety and insomnia. atives in low dosesence on benzodiaen orally or by intriazepines are excre (COC) is a potenogical effects indogical effects are ad in the urine prim, which is much lo

e use or exposure.enedioxymethame effects of 3,4-me

blurred visiohylenedioxymethahylenedioxymethalizes including met

done (MTD) is a syychological effectsone may cause coiological half-life ofs (OPI/MOP) suchat high doses pro

sed coordination, lized to 6-acetylm

ne and morphine morphine, and/or c

done (OXY) is a sdative effects, and lized by demethylaone in urine is expyclidine (PCP), cos an anesthetic anous injection. It pof euphoria and vis

in moderate amouydrocannabinol (Td, it produces euphcoordination, imp

ate. The primary m

S

e in each control d for the correspon

ble in each control at specific test zone

ble in the control “ned and run to re-u purchased the pr

ng attributed to line

ear lighter or thiHOW DARK OR FA

provides only a pobtain a confirm

pectrometry (GC/Med to any drug tese Test? positive test would ommon causes of plements may cause Test? negative test is thane specimens may

n developed for testances other than

samples may prodtes. If a sample is stative screening asn

on regarding to t

phetamine (AMP/Malertness, and se

otic behavior, and cg with deaminated urine specimens fr

classified as centrntoxication. Sympfrequency, and duBarbiturates are tically excrete in urare central nervo

In general, benzods. The use of benzzepines can deveramuscular or intrreted in the urine ant central nervous duced by using accompanied by inarily as benzoylec

onger than that of

mphetamine (MDMthylenedioxymetha

on, vomiting, amphetamine amphetamine is tathylenedioxyamphynthetic analgesic s induced by usingoma or even deathf 15-60 hours. h as heroin, morpoduces euphoria a

disrupted decisiomorphine (6-AM), glucuronide. Thuscodeine use. emi-synthetic opiohas a dependenc

ation to noroxycodected to be similarommonly known aagent and a veterproduces hallucinasual distortions. It isunts (10% of the dTHC) is generally

horic effects. Abuseaired short term m

metabolite of marij

region. If no colornding drug of that s

region and the appe is absent or belo

“C” region or a co-evaluate the specroduct. When ca

e color intensity or

inner than otherAINT, SHOULD Breliminary analytic

med analytical reMS) is the preferre

t result, particularl

be an instance wa false positive tese a false positive

at the initial drug iy cause a false neg

sting urine sampleurine. uce erroneous ressuspected of beingssay. It is not desig

the use of this p

MET) and their mense of increased cardiac dysrhythmor hydroxylated d

rom most metham

ral nervous systemptoms include slurrration of use, tolertaken orally, or brine as parent comus system (CNS) diazepines act as zodiazepines can lop if high doses o

ravenous injectionas conjugates and system stimulant cocaine are eup

ncreased heart ratcgonine in a short cocaine (0.5 to 1.

MA) is classified asamphetamine useand increased may cause

aken orally in tablehetamine (MDA).drug originally useg methadone are h. Methadone is ta

hine, and codeineand release from aon making, decre

morphine, and ms, the presence o

oid with a structurace liability similar todone and oxymorpr to that of other o

as “angel dust” anrinary tranquilizer. ations, lethargy, dis well absorbed foose). accepted to be th

ers exhibit central memory, anxiety, uana excreted in

r band appears in specific test zone.

propriate test “T” reow the detection lim

lor band is only vicimen. If test still plling, be sure to pr

width. Any visibl

r lines. ANY COE INTERPRETED

cal test result. A moesult. GC/MS is d confirmatory mey when preliminary

here a substance est are cross react

test result with thi

s present but isn’tgative result.

es only. No other f

sults. Strong oxidizg adulterated, obtagned to determine

product, please c

etabolites are poteenergy and powe

mias. Methamphetaderivatives. Methammphetamine users

m depressants. Thred speech, loss orance, physical de

by intravenous anmpound and metab

depressants commhypnotics in high result in drowsine

of the drug are give, and are extensivmetabolites. and a local anesthphoria, confidencete, dilation of the pperiod of time. Be5 hour), and can b

s both a stimulant include jaw clenc

blood pressheart failu

ets or capsules an

ed for the treatmenanalgesia, sedati

aken orally or intra

e, are central nervanxiety. Physical deased respiration, morphine glucurof morphine or mo

al similarity to codeo morphine. Oxycophone followed bypioids such as mod “crystal cycloneThe drug is abu

sorientation, loss ollowing all routes o

he principle activenervous system eparanoia, depressthe urine is 11-no

the appropriate te

egion, it indicates mit of the test.

isible in the test “Tprovides an invalirovide the lot numb

le line is considere

OLORED LINE VID AS A NEAGATIVore specific alterna

the preferred cethod. Clinical cony positive results a

is identified incorrtants. Certain foodis product.

t detected by USc

fluids have been e

zing agents such aain a new sample

e the quantitative c

contact US Diag

ent central nervouer. Responses froamine and amphetmphetamine also contain both unch

hese products proof motor coordinatependence and psnd intramuscular ibolites. monly prescribed doses, as anxiolytss and confusion. en over a prolongevely oxidized in th

hetic found in the lee and sense of

pupils, fever, tremonzoylecgonine hasbe generally detec

and a hallucinogeching, teeth grindinure and heaure or exnd excreted in urin

nt of narcotic addicon, and respiratoavenously and is m

vous system (CNSdependence is app

hypothermia andnide. Codeine als

orphine glucuronid

eine. It producesodone is most oftey glucuronidation. Torphine. e", is an arylcyclohsed by oral or naof coordination, tr

of administration. U

e component in mffects, altered moosion, confusion, hor-Δ-9-tetrahydroca

est “T” region, a p

that the concentra

T” region, the test d result, please cober for the test.

ed to be a line.

ISIBLE IN THE TVE RESULT. ative chemical meconfirmatory methnsideration and proare indicated.

rectly by UScreen2

ds and medicines

creen2 Drugs of Ab

evaluated. DO NO

as bleach (hypochin a different, unus

concentration of dr

gnostics 888-669

us system stimulanom chronic use catamine are excretemetabolize to amphanged parent dru

oduce a state of intion and impaired sychological depeninjections. Membe

for the short-term tics in moderate dPsychological an

ed period. Benzode liver to metabol

eaves of the coca increased energ

ors and sweating. Cs a biological half-cted for 24 to 60 h

en. Like methampng, dilated pupils, prt rate. Overd

xtreme heat ne as parent comp

ction and pain manry depression. Ovmetabolized in the

S) depressants. Tparent in users and coma. Heroin so partially metab

de in the urine ca

s potent euphoria, en administered orThe window of de

hexylamine that isasal ingestion, smrance-like ecstaticUnchanged PCP is

marijuana. When inod and sensory peallucinations and annabinol-9-carbo

preliminary

ation of the

is invalid. ontact the

TEST “T”

ethod must hod. Gas ofessional

2 Drugs of , diet plan

buse Cup.

T use this

lorite) can sed, cup. rugs or the

9-4337

nts. Acute an include ed in urine phetamine ug and the

ntoxication judgment.

ndence on ers of the

treatment doses, and d physical

diazepines lites. Most

plant. The gy. These Cocaine is -life of 5 to hours after

phetamine, perspiring, dose of

stroke. pound and

nagement. verdose of e liver and

The use of d leads to is quickly bolizes to n indicate

analgesic rally and is tection for

s originally moking, or c states, a s excreted

ngested or erceptions, increased

oxylic acid.

TheTricof cby Bupnamcomsubidedemelimtho

QUUseconThorecPosass

PE

Acc112abudru

A

B

B

C

TH

M

M

M

M

P

TC

e elimination of THcyclic Antideprescausing serious cainjection. TCAs anprenorphine (BUmes Subutex™, mbination with Nabstitution treatmenntical substance tmonstrates a lowemination of a singught to be approxi

UALITY CONTROLers should follow tntrol materials. ough there is an incommended as gositive and negativesay procedure sho

RFORMANCE CH

curacy20 (eighty of each use Test. Each Ug-free, less than h

Drug UScreen2 Test Result

AMP Viewer A +-

Viewer B +-

Viewer C +-

AR Viewer A +-

Viewer B +-

Viewer C +-

ZO Viewer A +-

Viewer B +-

Viewer C +-

OC Viewer A +-

Viewer B +-

Viewer C +-

HC Viewer A +-

Viewer B +-

Viewer C +-

MET Viewer A +-

Viewer B +-

Viewer C +-

MDMA Viewer A +-

Viewer B +-

Viewer C +-

MOP Viewer A +-

Viewer B +-

Viewer C +-

MTD Viewer A +-

Viewer B +-

Viewer C +-

CP Viewer A +-

Viewer B +-

Viewer C +-

CA Viewer A +-

Viewer B +-

Viewer C +

HC and metabolitesssants (TCA) haveardiac complicationnd their metaboliteP) is a potent anaBuprenex™, Tem

aloxone HCl. Thernt is a form of meto the drug normaer level of physicale-dose of the druimately 3 days.

Lthe appropriate fed

nternal procedural ood laboratory tese control should guld be adopted.

HARACTERISTICS

drug) clinical urineUScreen2 test washalf the cutoff, nea

Drug Less than h

Free

acutoff ntraticonce o

GC/MS ana0 0

10 18 0 0

10 18 0 0

10 18 0 0

10 10 0 0

10 10 0 0

10 10 0 0 10 10 0 0 10 10 0 0 10 10 0 0 10 10 0 0 10 10 0 0 10 10 0 0 10 12 0 0 10 12 0 0 10 12 0 0 10 16 0 0 10 16 0 0 10 16 0 0 10 10 0 0 10 10 0 0 10 10 0 0 10 19 0 0 10 19 0 0 10 19 0 0 10 12 0 0 10 12 0 0 10 12 0 0 10 13 0 0 10 13 0 0 10 13 0 0 10 19 0 0 10 19 0 0

s in urine is highly e been prescribedns, TCAs can be ls are excreted in ulgesic often used i

mgesic™ and Subrapeutically, Bupreedical care offeredally used. In subsal dependence. Thug can take as lon

deral state, and lo

control line in the sting practice to cgive the expected

S

e specimens weres read by three vier cutoff negative, n

alf the

on bylysis

Near Cutoff (Between 50

the cutoff acutoff conce

2102101112182181191

191

192

181

192

182

182

161

171

171

132

121

132

182

181

191

10291

102

161

172

162

152

152

151

101

101

y dependent on fre for depression anethal if misused aurine (mostly in thein the treatment ofboxone™; all of enorphine is used d to opiate addictsstitution therapy, he plasma half-lifeng as 6 days, the

cal guidelines con

test device of Conconfirm the test p

results. When te

e analyzed by GC-ewers. Samples wnear cutoff positive

Negative 0% below and the entration)

Near Cu(Betweeand 50%cutoff co

20

20

128

28

9

9

928

928

28

26

7

7

322

328

28

9

029

026

726

25

25

25

0

0

quency of drug usnd compulsive disoat high doses. TCAe form of metabolitf opioid addiction. which contain Bas a substitution

s (primarily heroinBuprenorphine is e of Buprenorphin

e detection window

ncerning the freque

ntrol region, the usprocedure and to sting the positive

-MS and by each were divided by coe, and high positive

utoff Positive en the cutoff % above the oncentration)

Highgra

eateboveconce

110110110

200

200

20020020020011011011018018018020020020020020020020020020019019019018018018010010010

se and physiology oorders. Because ofAs are taken orallytes) for up to ten dThe drug is sold u

Buprenorphine HCn treatment for opn addicts) based o

as effective as Mne is 2-4 hours. Ww for the parent d

ency of assaying e

se of external contverify proper testand negative con

corresponding USoncentration into fe. Results were as

h Positive r than 50%the cutoff

entration)

%Ag

29 100%0 95%29 100%0 95%29 100%0 97.5%20 100%0 95%20 100%0 95%20 100%0 97.5%20 100%0 97.5%20 100%0 97.5%20 100%0 95%29 100%0 97.5%29 100%0 95%29 100%0 95%22 100%0 95%22 100%0 97.5%22 100%0 97.5%20 100%0 97.5%20 100%0 95%20 100%0 97.5%20 100%0 95%20 100%0 95%20 100%0 97.5%20 100%0 97.5%20 100%0 95%20 100% 0 97.5%21 100%0 95%21 100%0 97.5%21 100% 0 95% 22 100%0 95%22 100%0 95%22 100%0 95%30 100%0 97.5%30 100%0 97.5%30 100%

of the user. f the possibility

y or sometimes days. under the trade Cl alone or in ioid addicts. A on a similar or Methadone but While complete drug in urine is

external quality

trols is strongly t performance. ntrol, the same

Screen2 drug of ive categories: s follows:

greement with GC/MS (95%CI)

(84.5% - 100%)(79.5% - 100%)(84.5% - 100%)

(79.5% - 100%)(84.5% - 100%)

% (82% - 100%)(84.5% - 100%)

(79.5% - 100%)(84.5% - 100%)

(79.5% - 100%)(84.5% - 100%)

% (82% - 100%)(84.5% - 100%)

% (82% - 100%)(84.5% - 100%)

% (82% - 100%)(84.5% - 100%)

(79.5% - 100%)% (84.5% - 100%)% (82% - 100%)

(84.5% - 100%)(79.5% - 100%)(84.5% - 100%)

(79.5% - 100%)(84.5% - 100%)

(79.5% - 100%)% (84.5% - 100%)% (82% - 100%)% (84.5% - 100%)% (82% - 100%)

(84.5% - 100%)% (82% - 100%)(l84.5% - 100%)(79.5% - 00%1 )(84.5% - 100%)

% (82% - 100%)(84.5% - 100%)

(79.5% - 100%)(84.5% - 100%)

(79.5% - 100%)(84.5% - 100%)

% (82% - 100%)% 84.5% - 100%)% (82% - 100%)

(84.5% - 100%)(79.5% - 100%)(84.5% - 100%)

% (82% - 100%)(84.5% - 100%)

(79.5% - 100%)(84.5% - 100%)

% (82% - 100%)(84.5% - 100%)

(79.5% - 100%)(84.5% - 100%)

(79.5% - 100%)(84.5% - 100%)

(79.5% - 100%)(84.5% - 100%)

(79.5% - 100%)(84.5% - 100%)

% (82% - 100%)(84.5% - 100%)

% (82% - 100%)(84.5% - 100%)

- 10 19 10 0 0 97.5% (82% - 100%)

R

OXY Viewer A + 0 0 2 19 21 100% (84.5% - 100%) - 10 20 8 0 0 95% (79.5% - 100%)

Viewer B + 0 0 2 19 21 100% (84.5% - 100%) - 10 20 8 0 0 95% (79.5% - 100%)

Viewer C + 0 0 1 19 21 100% (84.5% - 100%) - 10 20 9 0 0 97.5% (82% - 100%)

BUP Viewer A + 0 0 1 16 24 100% (84.5% - 100%) - 10 18 11 0 0 97.5% (82% - 100%)

Viewer B + 0 0 1 16 24 100% (84.5% - 100%) - 10 18 11 0 0 97.5% (82% - 100%)

Viewer C + 0 0 1 16 24 100% (84.5% - 100%) - 10 18 11 0 0 97.5% (82% - 100%)

recision and Sensitivity

sensitivity, each drug sample was analyzed at the following concentrations: cutoff - 100%,

deter r lot

P To investigate the precision andcutoff - 75%, cutoff - 50%, cutoff - 25%, cutoff, cutoff +25%, cutoff + 50%, cutoff + 75% and the cutoff + 100%. All concentrations were confirmed with GC-MS. The study was performed 2 runs /day and lasted 25 days using three different lots of the corresponding UScreen2 drugs of abuse test. In total, 3 operators participated in the study of the corresponding UScreen2 drugs of abuse test. Each of the 3 operators tested 2 aliquots at each concentration for each lot per day (2 runs /day), for a total of 50 determinations per concentration per lot of the corresponding UScreen2 drugs of abuse test.

Drug test Approximate concentration Number of Result (Negative/Positive) of sample (ng/mL) minations pe Lot 1 Lot 3 Lot 2

AMP 0 50 50/0 50/0 50/0 2 50 50 50/0 50/0 50/0 500 50 50/0 50/0 50/0 750 50 50/0 50/0 50/0 1000 50 5/45 5/45 4/46 1250 50 0/50 0/50 0/50 1500 50 0/50 0/50 0/50 1750 50 0/50 0/50 0/50 2000 50 0/50 0/50 0/50

BAR 0 50 50/0 50/0 50/0 75 50 50/0 50/0 50/0

150 50 50/0 50/0 50/0 225 50 50/0 50/0 50/0 300 50 7/43 5/45 5/45 375 50 0/50 0/50 0/50 450 50 0/50 0/50 0/50 525 50 0/50 0/50 0/50 600 50 0/50 0/50 0/50

BZO 0 50 50/0 50/0 50/0 75 50 50/0 50/0 50/0

150 50 50/0 50/0 50/0 225 50 50/0 50/0 50/0 300 50 7/43 6/44 5/45 375 50 0/50 0/50 0/50 450 50 0/50 0/50 0/50 525 50 0/50 0/50 0/50 600 50 0/50 0/50 0/50

COC 0 50 0/50 0/50 0/50 75 50 50/0 50/0 50/0

150 50 50/0 50/0 50/0 225 50 50/0 50/0 50/0 300 50 5/45 5/45 5/45 375 50 0/50 0/50 0/50 450 50 0/50 0/50 0/50 525 50 0/50 0/50 0/50 600 50 0/50 0/50 0/50

THC 0 50 50/0 50/0 50/0 1 2.5 50 50/0 50/0 50/0 25.0 50 50/0 50/0 50/0 37.5 50 50/0 50/0 50/0 50.0 50 5/45 6/44 5/45 62.5 50 0/50 0/50 0/50 75.0 50 0/50 0/50 0/50 87.5 50 0/50 0/50 0/50 100.0 50 0/50 0/50 0/50

MET 0 50 50/0 50/0 50/0 2 50 50 50/0 50/0 50/0 500 50 50/0 50/0 50/0 750 50 50/0 50/0 50/0 1000 50 4/46 5/45 5/45 1250 50 0/50 0/50 0/50 1500 50 0/50 0/50 0/50 1750 50 0/50 0/50 0/50 2000 50 0/50 0/50 0/50

MDMA 0 50 50/0 50/0 50/0 1 25 50 50/0 50/0 50/0 250 50 50/0 50/0 50/0 375 50 50/0 50/0 50/0 500 50 6/44 5/45 6/44 625 50 0/50 0/50 0/50 750 50 0/50 0/50 0/50 875 50 0/50 0/50 0/50 1000 50 0/50 0/50 0/50

MOP 0 50 50/0 50/0 50/0 75 50 50/0 50/0 50/0

150 50 50/0 50/0 50/0 225 50 50/0 50/0 50/0 300 50 5/45 6/44 5/45 375 50 0/50 0/50 0/50 450 50 0/50 0/50 0/50 525 50 0/50 0/50 0/50 600 50 0/50 0/50 0/50

MTD 0 50 50/0 50/0 50/0 75 50 50/0 50/0 50/0

150 50 50/0 50/0 50/0 225 50 50/0 50/0 50/0 300 50 6/44 4/46 5/45375 50 0/50 0/50 0/50450 50 0/50 0/50 0/50525 50 0/50 0/50 0/50600 50 0/50 0/50 0/50

PCP 0 50 50/0 50/0 50/06.25 50 50/0 50/0 50/012.5 50 50/0 50/0 50/018.75 50 50/0 50/0 50/0

25 50 5/45 4/46 5/453 51.2 50 0/50 0/50 0/5037.5 50 0/50 0/50 0/5043.75 50 0/50 0/50 0/50

50 50 0/50 0/50 0/50TCA 0 50 50/0 50/0 50/0

250 50 50/0 50/0 50/0500 50 50/0 50/0 50/0750 50 50/0 50/0 50/01000 50 5/45 6/44 5/451250 50 0/50 0/50 0/501500 50 0/50 0/50 0/501750 50 0/50 0/50 0/502000 50 0/50 0/50 0/50

OXY 0 50 50/0 50/0 50/025 50 50/0 50/0 50/050 50 50/0 50/0 50/075 50 50/0 50/0 50/0

100 50 6/44 6/44 5/45125 50 0/50 0/50 0/50150 50 0/50 0/50 0/50175 50 0/50 0/50 0/50200 50 0/50 0/50 0/50

BUP 0 50 50/0 50/0 50/02.5 50 50/0 50/0 50/05.0 50 50/0 50/0 50/07.5 50 50/0 50/0 50/0

10.0 50 6/44 4/46 4/4612.5 50 0/50 0/50 0/5015.0 50 0/50 0/50 0/5017.5 50 0/50 0/50 0/50 20.0 50 0/50 0/50 0/50

pecificity and cross reactivity device was used to test various drugs, drug metabolites and other components of the

Cocaine (COC)

STo test the specificity of the test, the testsame class that are likely to be present in urine. All the components were added to drug-free normal human urine. The following structurally related compounds produced positive results with the test when tested at levels equal to or greater than

the concentrations listed below.

Amphetamine (AMP) Concentration Concentration

(ng/ml) (ng/ml) d-Amphetamin 1,000 Benzoylecgonine 300d.l-Amphetamine 3,000 Cocaine HCl 7501-Amphetamine 50,000 Cocaethylene 12,500(+/-) 3,4-methylenedioxyamphetamine (MDA) 5,000 Ecgonine 32,000Phentermine 3,000 Methadone (MTD)d-methamphetamine >100,000 Methadone 300l-methamphetamine >100,000 Doxylamine 50,0003,4-Methylenedioxyethylamphetamine(MDE) 100,000 Methamphetamine (MET)(+/-)3,4-methylenedioxumethamphetamine (MDMA) 100,000 D(+)-Methamphetamine 1,000Secobarbital (BAR) D-Amphetamine 50,000Secobarbital 300 Chloroquine 50,000Amobarbital 300 (+/-)-Ephedrine 50,000Alphenol 150 (-)-Methamphetamine 25,000Aprobarbital 200 (+/-)3,4-methylenedioxumethamphetamine(MDMA) 2,000Butabarbital 75 β-Phenylethylamine 50,000Butathal 100 Trimethobenzamide 10,000Butalbital 2,500 Methylenedioxymethamphetamine (MDMA)Cyclopentobarbital 600 3,4-Methylenedioxymethamphetamine HCl (MDMA) 500Pentobarbital 300 3,4-Methylenedioxyamphetamine HCl (MDA) 3,000Phenobarbital 10,000 3,4-Methylenedioxyethylamphetamine (MDE) 300Oxazepam(BZO) Morphine (MOP)Oxazepam 300 Morphine 300Alprazolam 200 Codeine 300a-Hydroxyalprazolam 1,500 Ethyl Morphine 300Bromazepam 1,500 Hydrocodone 5,000Chlordiazepoxide 1,500 Hydromorphone 5,000Clonazepam HCl 800 Morphinie-3-β-d-glucuronide 1,000Clobazam 100 Thebaine 30,000Clonazepam 800 Phencyclidine (PCP)Clorazepate dipotassium 200 Phencyclidine 25Delorazepam 1,500 4-Hydroxyphencyclidine 1 0250Desalkylflurazepam 400 Notriptyline (TCA)Diazepam 200 Notriptyline 1,000Estazolam 2,500 Nordoxepine 1,000Flunitrazepam 400 Trimipramiine 3,000D,L-Lorazepam 1,500 Amitriptyline 1,500Midazolam 12,500 Promazine 1,500Nitrazepam 100 Desipramine 200Norchlordiazepoxide 200 Imipramine 400Nordiazepam 400 Clomipramine 12,500Temazepam 100 Doxepine 2,000Trazolam 2,500 Maprotiline 2,000Cannabinoids (THC) Promethazine 25,00011-nor-Δ9-THC-9-COOH 50 Oxycodone(OXY)11-nor-Δ8-THC-9-COOH 30 Oxycodone 10011-hydroxy-Δ9-Tetrahydrocannabinol 2,500 Dihydrocodeine 20,000Δ8- Tetrahydrocannabinol 7,500 Codeine 100,000Δ9- Tetrahydrocannabinol 10,000 Hydromorphone 100,000Cannabinol 100,000 Morphine ＞100,000Cannabidiol 100,000 Acetylmorphine ＞100,000Buprenorphine(BUP) Buprenorphine ＞100,000Buprenorphine 10 Ethylmorphine ＞100,000Buprenorphine -3-D-Glucuronide 15Norbuprenorphine 20Norbuprenorphine 3-D-Glucuronide 200

Adulteration/Specimen Validity Test tor in an alkaline medium to form a purplish-brown color complex if creatinine in

sed on the pKa change of certain pretreated polyelectrolytes in relation to the ionic

other oxidizing agents react with an oxidant indicator to form a color complex. Observation of a blue-green,

ntain substances that could potentially interfere with the test. The following compounds were

Fenoprofen xolinic acid

de (except OXY test) except BZO test)

O test) l (except BZR, OXY test) st)

Cr: Creatinine reacts with a creatinine indicathe urine is present at the normal level. The color intensity is directly proportional to the concentration of creatinine. A urine sample with creatinine concentration of less than 20 mg/dL produces a very light, or no pad color change, which indicates adulteration in the from of specimen dilution. Adulteration/Specimen Validity Test

S.G.: The specific gravity test is baconcentration. The pad colors will change from dark blue to blue-green in urine of low ionic concentration to green and yellow-green in urine of higher ionic concentration. A urine specific gravity below 1.003 or above 1.025 is considered abnormal.

OX: Bleach or medium to dark brown, or orange color indicates adulteration with bleach or other oxidizing agents.

nterfering substances IClinical urine samples may coadded to drug-free urine, with a drug concentration 25% below the cutoff, and urine with a drug concentration 25% above the cutoff and were tested with the UScreen2 drugs of abuse test. All potential interferents were added at a concentration of 100 µg/mL. None of the urine samples showed any deviation from the expected results.

cetominophen (4-Acetamidophenol) A(except OXY test) Acetophenetidin

mi

OFurosemide Oxymetazoline

N-Acetylprocaina Gentisic acid Papaverine Acetylsalicylic acid Hydralazine ( Penicillin-G Aminopyrine Hydrochlorothiazide (except BZ PentobarbitaAmoxicillin Hydrocodone (exceptBZO,MOP,OXY te Perphenazine Ampicillin Hydrocortisone Phenelzine Apomorphine (except PCP, OXY tests) O-Hydroxyhippuric acid PhencyclidineAspartame 3-Hydroxytyramine Prednisone Atropine (except BAR test) test) )

P, BAR test)

ine (except COC,OXY test) R tests)

e id xcept TCA, OXY tests) except BAR, OXY tests)

test) one, 3-acetate (except AMP,

M ,

except MOP, BZO, OXY tests)

costerone e

xcept MOP, BZO, OXY tests) (except AMP, BAR tests)

dramine ter

ZO test) BZO, OXY tests)

Ibuprofen (except OXY Procaine (except BZO, MOP, OXY testsBenzilic acid D,L-Isoproterenol (except AM DL-Propranolol

e (except OXY, test) Benzoic acid Isoxsuprine xcept OXY test)

D-PropoxyphenBenzoylecgon Ketamine (e D-Pseudoephedrine(except AMP, BABilirubin

diol (except THC, OXY tests) Ketoprofen Quinine

e Cannabi Labetalol RanitidinChloralhydrate

ol Loperamid Salicylic ac

Chloramphenic Maprotiline (e Secobarbital (Chlorothiazide

e Meperidine (except THC, OXY tests) Serotonin (5- Hydroxytyramine)

Chlorpromazin Meprobamate xcept MTD, OXY tests)

Sulfamethazine Chlorquine Methadone (e Sulindac

ocortisCholesterol Methoxyphenamine (exceptAMP,BAR TetrahydrBAR, OXY tests) Tetrahydrocortisone 3-(β-Dglucuronide) (except Clonidine orphinie-3-β-d-glucuronide (except BZO

MOP tests) Nalidixic acid

AMP, BAR, OXY tests) Tetrahydrozoline Codeine (

Cortisone e

Naloxone

Thiamine ne (-) Cotinin Naltrexone Thioridazi

Creatinine Naproxen e

Triamterene Deoxycorti Niacinamid DL-Tyrosine Dextromethorphan Nifedipine

(eTrifluoperazin

Diclofenac Norcodein Trimethoprim nDiflunisal Norethindrone

hene D L-Tryptopha

Digoxin y

D-Norpropoxyp Tyramine (except AMP, BAR tests) Diphenh Noscapine

mine Uric acid

l Ecgonine methyl es D,L-Octopa VerapamiErythromycin (except B Oxalic acid

(exceptZomepirac

β-Estradiol (except BZO test) Oxazepam

IBLIOGRAPHY OF SUGGESTED READING

micals in Man. Biomedical Publications, Davis, CA, 1982. ew

ug Abuse (NIDA),

B

aselt, R.C. Disposition of Toxic Drugs and CheBEllenhorn, M.J. and Barceloux, D. G Medical Toxicology. Elservier Science Publishing Company, Inc., NYork, 1988 Gilman, A. G., and Goodman, L. S. The Pharmacological Fluids, in Martin WR(ed): Drug Addiction I, New York, Spring – Verlag, 1977. Harvey, R.A., Champe, P.C. Lippincotts Illustrated Reviews. Pharmacology. 91-95, 1992. Hawwks RL, CN Chiang. Urine Testing for drugs of Abuse. National Institute for DrResearch Monography 73, 1986 Hofmann F.E., A Handbook on Drug and Alcohol Abuse: The Biomedical Aspects, New York, Oxford

Version 11/28/2012

University Press, 1983. McBay, A. J. Clin. Chem. 33,33B-40B, 1987. INDEX OF SYMBOLS