university of toronto t-space€¦ · web viewtable s2. results from a tukey-kramer post-hoc tests...
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Supplementary Material
Supplementary Tables
Table S1. Source information for estimated percent germination and dormancy breaking
information, used to determine the number of seeds per petri dish and additional treatment in our
experiment.
Species CodeEstimated Germ
PercentageGerm Percentage/Dormancy Breaking info:
Amsinckia
menziesii 40%
http://www.fs.fed.us/rm/pubs_other/
rmrs_2010_forbis_t001.pdf
Atriplex lentiformis10%
http://npn.rngr.net/renderNPNProtocolDetails?
selectedProtocolIds=chenopodiaceae-atriplex-1394
Brassica nigra 60-80% based on our previous germination trials
Bromus diandrus 60-80% based on our previous germination trials
Bromus
madritensis 60-80% based on our previous germination trials
Elymus
condensatus 50% based on IRC seed farm observation
Encelia californica 30% based on our previous germination trials
Eriogonum
fasciculatum
50%
http://www.fs.fed.us/psw/publications/beyers/
psw_2010_beyers013(montalvo)eriogonumfasciculatum
Erodium
cicutarium 50% based on our previous germination trials
Eschscholzia
californica 40%
http://npn.rngr.net/renderNPNProtocolDetails?
selectedProtocolIds=papaveraceae-eschscholzia-605
Lupinus bicolor40%
http://npn.rngr.net/renderNPNProtocolDetails?
selectedProtocolIds=fabaceae-lupinus-636
Lupinus
succulentus 40% based on our previous germination trials
Malosma laurina 50%
https://books.google.com/books?
id=ZnBRmdUU_3UC&pg=PA957&lpg=PA957&dq=c
old+stratifying+seeds+malosma&source=bl&ots=Tg1d
FmEow_&sig=hIakRlMRDmvDsHrvcQ_E__8dZSI&hl
=en&sa=X&ved=0ahUKEwjI_7mLrpjKAhUOxmMKH
azLC6YQ6AEIMTAD#v=onepage&q=cold
%20stratifying%20seeds%20malosma&f=false
Penstemon
spectabilis 20%
http://digitalcommons.unl.edu/cgi/viewcontent.cgi?
article=1664&context=agronomyfacpub
Peritoma arborea 20% based on anecdotal planting success from IRC employee
Phacelia cicutaria 20% based on our previous germination trials
Plantago erecta 50% based on our previous germination trials
Rhus integrifolia50%
http://www.cnpssd.org/germination%20rhus
%20integ.pdf
Stipa pulchra 30% based on our previous germination trials
Table S2. Results from a Tukey-Kramer post-hoc tests on the effect of (A) species, (B)
treatment, and (C) species-by-treatment interaction on germination timing. Shared letters
indicate no significant differences amongst groups (P<0.05 significance level). Mean and SE
values are also provided.
A.
Species
Mean Days to
Germination Standard Error Letter Group
Rhus integrifolia 25.70 0.9138 A
Peritoma arborea 21.45 0.5977 B
Malosma laurina 19.80 0.6089 B
Eriogonum
fasciculatum15.84
0.5752 C
Elymus condensatus 15.51 0.3663 C
Encelia californica 15.06 0.4719 C
Atriplex lentiformis 14.27 0.2475 C
Eschscholzia
californica13.76
0.4415 C
Stipa pulchra 11.59 0.2951 D
Bromus diandrus 8.96 0.3112 E
Amsinckia menziesii 8.77 0.3258 E
Bromus madritensis 7.73 0.325 EF
Lupinus bicolor 7.14 0.3715 FG
Brassica nigra 6.64 0.2931 FG
Erodium cicutarium 6.43 0.2577 FG
Lupinus succulentus 5.77 0.2823 G
Rhus integrifolia 25.70 0.9138 A
B.
Treatment
Mean Days to
Germination Standard Error Letter Group
soil 13.8748 13.87 A
ppfm 12.2493 12.25 B
control 12.2053 12.21 B
C.
Species Treatment
Mean Days
to
Germination Standard Error Letter Group
Rhus
integrifolia soil26.02 1.622 A
Rhus
integrifolia ppfm25.85 1.871 A
Rhus
integrifolia control25.23 1.149 A
Peritoma
arborea soil24.38 1.115 A
Malosma
laurina ppfm20.40 1.004 ABC
Malosma
laurina control20.11 0.871 AB
Peritoma
arborea ppfm20.05 0.856 ABD
Peritoma
arborea control19.92 1.085 ABC
Malosma
laurina soil18.88 1.228 ABCE
Eschscholzia
californica soil16.89 0.921 BCEF
Eriogonum
fasciculatum soil16.69 0.921 BCEFG
Encelia
californica soil16.69 1.005 BCEFG
Atriplex
lentiformis soil16.26 0.410 CE
Elymus
condensatus soil16.01 0.581 BCEF
Eriogonum
fasciculatum control15.99 1.029 BCEFGH
Elymus
condensatus control15.92 0.655 BCEF
Eriogonum
fasciculatum ppfm14.85 1.004 CDEFGHI
Elymus
condensatus ppfm14.59 0.615 EFGH
Encelia
californica control14.50 0.714 EFGH
Encelia
californica ppfm14.00 0.649 EFGHI
Atriplex
lentiformis ppfm13.77 0.397 FGH
Eschscholzia
californica ppfm12.87 0.649 FGHIJ
Atriplex
lentiformis control12.79 0.404 GHI
Stipa
pulchra soil11.93 0.516 HIJK
Stipa
pulchra ppfm11.72 0.483 HIJK
Eschscholzia
californica control11.52 0.650 HIJKLM
Stipa
pulchra control11.12 0.471 IJKL
Amsinckia
menziesii soil9.75 0.662 JKLMN
Brassica
nigra soil9.39 0.525 KLMN
Bromus
diandrus soil9.35 0.568 KLMNO
Bromus
diandrus control9.21 0.496 KLMN
Amsinckia
menziesii control8.56 0.483 LMNOP
Bromus
diandrus ppfm8.31 0.490 NOPQ
Bromus
madritensis soil8.21 0.545 MNOPQR
Amsinckia
menziesii ppfm8.00 0.471 NOPQR
Lupinus
bicolor soil7.99 0.668 LMNOPQRST
Erodium
cicutarium soil7.61 0.422 NOPQRS
Bromus
madritensis ppfm7.60 0.538 NOPQRST
Bromus
madritensis control7.38 0.549 NOPQRST
Lupinus
bicolor ppfm7.21 0.637 NOPQRST
Lupinus
bicolor control6.20 0.573 OPQRST
Lupinus
succulentus soil5.92 0.493 PQRST
Lupinus
succulentus control5.89 0.467 QRST
Erodium
cicutarium ppfm5.84 0.419 QRST
Erodium
cicutarium control5.83 0.427 QRST
Lupinus
succulentus ppfm5.51 0.441 RST
Brassica
nigra ppfm5.41 0.464 ST
Brassica
nigra control5.12 0.469 T
Rhus
integrifolia soil26.02 1.622 A
Rhus
integrifolia ppfm25.85 1.871 A
Rhus
integrifolia control25.23 1.149 A
Table S3. Results from a Tukey-Kramer post-hoc test analyzing the effect of (A) species, (B)
treatment, and (C) species-by-treatment interaction percent germination. Shared letters indicate
no significant differences amongst groups (P<0.05 significance level). Mean and SE values are
also provided.
A.
Species
Mean %
Germinatio
n SE Group
Brassica
nigra1.313 0.032
A
Erodium
cicutarium1.270 0.032
AB
Bromus
diandrus1.137 0.032
BC
Bromus
madritensis0.998 0.032
CD
Atriplex
lentiformis0.947 0.032
D
Stipa
pulchra0.917 0.032
D
Lupinus
succulentus0.871 0.032
D
Amsinckia
menziesii0.659 0.032
E
Lupinus 0.597 0.032 E
bicolor
Elymus
condensatus0.543 0.032
EF
Eschscholzi
a californica0.441 0.032
FG
Encelia
californica0.415 0.032
FG
Peritoma
arborea0.333 0.032
GH
Eriogonum
fasciculatum0.311 0.032
GH
Malosma
laurina0.303 0.032
GH
Rhus
integrifolia0.228 0.032
H
B.
Treatment
Mean %
Germinatio
n SE
Letter
Group
ppfm 0.750 0.014 A
control 0.733 0.014 A
soil 0.632 0.014 B
C.
Species Treatment Mean % SE Letter Group
Germination
Brassica nigra ppfm 1.459 0.055 A
Brassica nigra control 1.426 0.055 A
Erodium cicutarium ppfm 1.314 0.055 AB
Bromus diandrus ppfm 1.259 0.055 ABC
Erodium cicutarium soil 1.257 0.055 ABCD
Erodium cicutarium control 1.240 0.055 ABCDE
Bromus diandrus control 1.221 0.055 ABCDEF
Brassica nigra soil 1.053 0.055 BCDEFG
Bromus madritensis ppfm 1.017 0.055 BCDEFGH
Bromus madritensis soil 0.993 0.055 CDEFGH
Bromus madritensis control 0.984 0.055 CDEFGH
Stipa pulchra control 0.972 0.055 CDEFGH
Atriplex lentiformis ppfm 0.969 0.055 CDEFGH
Lupinus succulentus ppfm 0.949 0.055 DEFGHI
Atriplex lentiformis control 0.945 0.055 EFGHI
Stipa pulchra ppfm 0.932 0.055 EFGHI
Bromus diandrus soil 0.930 0.055 EFGHI
Atriplex lentiformis soil 0.926 0.055 FGHI
Lupinus succulentus control 0.863 0.055 GHIJ
Stipa pulchra soil 0.845 0.055 GHIJK
Lupinus succulentus soil 0.799 0.055 GHIJKL
Amsinckia menziesii ppfm 0.757 0.055 GHIJKLM
Amsinckia menziesii control 0.731 0.055 HIJKLM
Lupinus bicolor control 0.657 0.055 IJKLMN
Lupinus bicolor ppfm 0.585 0.055 JKLMNO
Elymus condensatus soil 0.581 0.055 JKLMNO
Lupinus bicolor soil 0.548 0.055 KLMNO
Elymus condensatus ppfm 0.544 0.055 KLMNOP
Encelia californica ppfm 0.509 0.055 LMNOP
Eschscholzia
californica ppfm0.508 0.055
LMNOP
Elymus condensatus control 0.503 0.055 LMNOP
Amsinckia menziesii soil 0.489 0.055 MNOPQ
Eschscholzia
californica control0.467 0.055
MNOPQ
Encelia californica control 0.452 0.055 MNOPQ
Peritoma arborea ppfm 0.412 0.055 NOPQ
Malosma laurina control 0.365 0.055 NOPQ
Eschscholzia
californica soil0.348 0.055
NOPQ
Eriogonum
fasciculatum soil0.346 0.055
OPQ
Rhus integrifolia control 0.314 0.055 OPQ
Malosma laurina ppfm 0.307 0.055 OPQ
Eriogonum ppfm 0.294 0.055 OPQ
fasciculatum
Peritoma arborea soil 0.294 0.055 OPQ
Eriogonum
fasciculatum control0.294 0.055
OPQ
Peritoma arborea control 0.291 0.055 OPQ
Encelia californica soil 0.283 0.055 OPQ
Malosma laurina soil 0.237 0.055 PQ
Rhus integrifolia ppfm 0.189 0.055 Q
Rhus integrifolia soil 0.181 0.055 Q
Table S4. Pearson correlation coefficients and P-values associated with mean germination
timing (MGT), mean percent germination (MPG), and seed weight (g).
Pearson Correlation Coefficients,
N=18 P-values
Control MPG MGT Weight_g Control MPG MGT Weight_g
MPG 1 -0.69735 -0.28855 MPG . 0.0013 0.2456
MGT 1 0.36179 MGT . 0.1401
Weight_g 1
Weight_
g .
PPFM MPG MGT Weight_g Control MPG MGT Weight_g
MPG 1 -0.6988 -0.26186 MPG . 0.0013 0.2939
MGT 1 0.52166 MGT . 0.0264
Weight_g 1
Weight_
g .
Soil MPG MGT Weight_g Control MPG MGT Weight_g
MPG 1 -0.38978 -0.2693 MPG . 0.1098 0.2799
MGT 1 0.54665 MGT . 0.0189
Weight_g 1
Weight_
g .