universal phylogenetic tree 1. 2 classification systems in the prokaryotae 1.microscopic morphology...
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Universal phylogenetic tree
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Classification Systems in the Prokaryotae
1. Microscopic morphology
2. Macroscopic morphology – colony appearance
3. Bacterial physiology
4. Serological analysis
5. Genetic and molecular analysis
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Bacterial Taxonomy Based on Bergey’s Manual
• Bergey’s Manual of Determinative Bacteriology – five volume resource covering all known prokaryotes– Classification based on genetic information –
phylogenetic– Two domains: Archaea and Bacteria– Five major subgroups with 25 different phyla
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Species and Subspecies• Species – a collection of bacterial cells which share an
overall similar pattern of traits in contrast to other bacteria whose pattern differs significantly
• Strain or variety – a culture derived from a single parent that differs in structure or metabolism from other cultures of that species (biovars, morphovars)
• Type – a subspecies that can show differences in antigenic makeup (serotype or serovar), susceptibility to bacterial viruses (phage type) and in pathogenicity (pathotype)
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Characteristics of CellsEukaryotic cells: animals, plants, fungi, and
protists– Contain membrane-bound organelles that
compartmentalize the cytoplasm and perform specific functions
– Contain double-membrane bound nucleus with DNA chromosomes
Prokaryotic cells: bacteria and archaea – No nucleus or other membrane-bound organelles
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Characteristics of Life• Reproduction and heredity – genome composed of
DNA packed in chromosomes; produce offspring sexually or asexually
• Growth and development• Metabolism – chemical and physical life processes• Movement and/or irritability – respond to
internal/external stimuli; self-propulsion of many organisms
• Cell support, protection, and storage mechanisms – cell walls, vacuoles, granules and inclusions
• Transport of nutrients and waste
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Comparison of Three Cellular Domains
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Prokaryotic Profiles
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Prokaryotic Profiles
• Structures that are essential to the functions of all prokaryotic cells are a cell membrane, cytoplasm, ribosomes, and one (or a few) chromosomes
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Cell Membrane Structure
• Phospholipid bilayer with embedded proteins – fluid mosaic model
• Functions in:– Providing site for energy reactions, nutrient processing, and
synthesis
– Passage of nutrients into the cell and the discharge of wastes• Cell membrane is selectively permeable
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Cell membrane structure
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Membrane Lipids
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Structure of a bacterial cell
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Structure of Cell Walls
• Determines cell shape, prevents lysis (bursting) or collapsing due to changing osmotic pressures
• Peptidoglycan is primary component:– Unique macromolecule composed of a
repeating framework of long glycan chains cross-linked by short peptide fragments
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Peptidoglycan
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Gram-Positive Cell Wall
• Thick, homogeneous sheath of peptidoglycan– 20-80 nm thick– Includes teichoic acid and lipoteichoic acid:
function in cell wall maintenance and enlargement during cell division; move cations across the cell envelope; stimulate a specific immune response
– Some cells have a periplasmic space, between the cell membrane and cell wall
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Gram-Negative Cell Wall• Composed of an outer membrane and a thin
peptidoglycan layer• Outer membrane is similar to cell membrane bilayer
structure – Outermost layer contains lipopolysaccharides and
lipoproteins (LPS)• Lipid portion (endotoxin) may become toxic when released
during infections• May function as receptors and blocking immune response• Contain porin proteins in upper layer – regulate molecules
entering and leaving cell
– Bottom layer is a thin sheet of peptidoglycan• Periplasmic space above and below peptidoglycan
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Comparison of Gram-Positive and Gram-Negative
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The Gram Stain
• Differential stain that distinguishes cells with a gram-positive cell wall from those with a gram-negative cell wall– Gram-positive - retain crystal violet and stain purple– Gram-negative - lose crystal violet and stain red from
safranin counterstain
• Important basis of bacterial classification and identification
• Practical aid in diagnosing infection and guiding drug treatment
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Nontypical Cell Walls• Some bacterial groups lack typical cell wall
structure, i.e., Mycobacterium and Nocardia– Gram-positive cell wall structure with lipid
mycolic acid (cord factor) • Pathogenicity and high degree of resistance to certain
chemicals and dyes• Basis for acid-fast stain used for diagnosis of infections
caused by these microorganisms
• Some have no cell wall, i.e., Mycoplasma – Cell wall is stabilized by sterols– Pleomorphic
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Extreme variation in shape of Mycoplasma pneumoniae
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Bacterial Internal Structures
• Cell cytoplasm:– Dense gelatinous solution of sugars, amino acids,
and salts– 70-80% water
• Serves as solvent for materials used in all cell functions
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• Chromosome– Single, circular, double-stranded DNA
molecule that contains all the genetic information required by a cell
– Aggregated in a dense area called the nucleoid • DNA is tightly coiled
Bacterial Internal Structures
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Chromosome structure
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Bacterial Internal Structures
• Plasmids– Small circular, double-stranded DNA– Free or integrated into the chromosome– Duplicated and passed on to offspring– Not essential to bacterial growth and metabolism– May encode antibiotic resistance, tolerance to toxic
metals, enzymes, and toxins– Used in genetic engineering - readily manipulated
and transferred from cell to cell
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Random microbe of the week!!!
Serratia marcescens
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• Ribosomes– Made of 60% ribosomal RNA and 40% protein– Consist of two subunits: large and small– Prokaryotic differ from eukaryotic ribosomes in
size and number of proteins– Site of protein synthesis
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Prokaryotic ribosome
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• Inclusions and granules– Intracellular storage bodies– Vary in size, number, and
content– Bacterial cell can use them
when environmental sources are depleted
– Examples: glycogen, poly -hydroxybutyrate, gas vesicles, sulfur and phosphate granules, particles of iron oxide
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• Cytoskeleton – Many bacteria possess an internal network of
protein polymers that is closely associated with the cell wall
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Bacterial Shapes, Arrangements, and Sizes
• Vary in shape, size, and arrangement but typically described by one of three basic shapes:– Coccus – spherical– Bacillus – rod
• Coccobacillus – very short and plump• Vibrio – gently curved
– Spirillum – helical, comma, twisted rod, • Spirochete – spring-like
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Common bacterial shapes
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Comparison of Spiral-Shaped Bacteria
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Bacterial Arrangements
• Arrangement of cells is dependent on pattern of division and how cells remain attached after division:– Cocci:
• Singles
• Diplococci – in pairs
• Tetrads – groups of four
• Irregular clusters
• Chains
• Cubical packets (sarcina)
– Bacilli:• Diplobacilli
• Chains
• Palisades
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Arrangement of cocci
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Arrangement of bacilli
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External Structures
• Appendages– Two major groups of appendages:
• Motility – flagella and axial filaments (periplasmic flagella)
• Attachment or channels – fimbriae and pili
• Glycocalyx – surface coating
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Flagella• 3 parts:
– Filament – long, thin, helical structure composed of protein Flagellin
– Hook – curved sheath
– Basal body – stack of rings firmly anchored in cell wall
• Rotates 360o
• Number and arrangement of flagella varies:– Monotrichous, lophotrichous, amphitrichous, peritrichous
• Functions in motility of cell through environment
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Flagella
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Flagellar Arrangements
1. Monotrichous – single flagellum at one end
2. Lophotrichous – small bunches emerging from the same site
3. Amphitrichous – flagella at both ends of cell
4. Peritrichous – flagella dispersed over surface of cell; slowest
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Electron micrographs of flagellar arrangements
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Flagellar ResponsesGuide bacteria in a direction in response to external
stimulus: Chemical stimuli – chemotaxis; positive and negativeLight stimuli – phototaxis
Signal sets flagella into rotary motion clockwise or counterclockwise:Counterclockwise – results in smooth linear direction –
runClockwise – tumbles
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The operation of flagella
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Chemotaxis in bacteria
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Periplasmic Flagella
• Internal flagella, enclosed in the space between the outer sheath and the cell wall peptidoglycan
• Produce cellular motility by contracting and imparting twisting or flexing motion
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Periplasmic flagella
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Fimbriae
• Fine, proteinaceous, hairlike bristles emerging from the cell surface
• Function in adhesion to other cells and surfaces
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Pili• Rigid tubular structure made of pilin protein
• Found only in gram-negative cells
• Function to join bacterial cells for partial DNA transfer called conjugation
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Glycocalyx• Coating of molecules external to the cell wall,
made of sugars and/or proteins• Two types:
1. Slime layer - loosely organized and attached
2. Capsule - highly organized, tightly attached
• Functions:– Protect cells from dehydration and nutrient loss
– Inhibit killing by white blood cells by phagocytosis, contributing to pathogenicity
– Attachment - formation of biofilms
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Biofilm on a catheter
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Archaea: The Other Prokaryotes• Constitute third Domain Archaea• Seem more closely related to Domain Eukarya than to
bacteria• Contain unique genetic sequences in their rRNA• Have unique membrane lipids and cell wall construction• Live in the most extreme habitats in nature,
extremophiles• Adapted to heat, salt, acid pH, pressure, and atmosphere• Includes: methane producers, hyperthermophiles,
extreme halophiles, and sulfur reducers
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Archaea
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Comparison of Three Cellular Domains
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