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Essential and toxic microelements in grains of Fusarium -infected maize and wheat genotypes: ICP-MS analysis. Zsuzsanna Kolbert, Attila Ördög, Ágnes Vashegyi, László Erdei. Two countries, one goal, joint success!. Materials and methods. Plant material : - PowerPoint PPT PresentationTRANSCRIPT
Two countries, one goal, joint success!
Essential and toxic microelements in grains of Fusarium-infected maize and wheat genotypes:
ICP-MS analysis
Zsuzsanna Kolbert, Attila Ördög, Ágnes Vashegyi, László Erdei
Materials and methods
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Sample preparation:•500 – 1000 mg from each sample•microwave digestion in nitric acid and hydrogen peroxide•dilution to 200 - 400 X
Measurement:•Microelement analysis (Mn, Fe, Co, Ni, Cu, Zn, As, Se, Mo, Cd, Pb) by ICP-MS device
Plant material:•2 maize genotypes (two sampling times)•13 wheat genotypes
Infection:•Fusarium graminearum•Fusarium culmorum•Fusarium verticillioides
The advantages of an ICP-MS
• More than 1000 samples in one week (up to 250 samples/day)• More than 15 000 data from the analysis • Lot of information in a few seconds (less than a second per
element)• User-friendly software (PlasmaLab), which makes the data
analysis easier and faster
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Microelement contents in the two maize genotypes
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GK-Sarolta GK-Boglár
Mn content in maize genotypes
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Two countries, one goal, joint success!
Fe content in maize genotypes
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Co content in maize genotypes
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Ni content in maize genotypes
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Cu content in maize genotypes
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Zn content in maize genotypes
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As content in maize genotypes
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Se content in maize genotypes
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Mo content in maize genotypes
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Cd content in maize genotypes
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Pb content in maize genotypes
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Microelement contents in 13 different wheat genotypes
• Manastur - TM• Sanandrei - TM• Faget-TM• Pietroasa- TM• Lovrin -TM• Aldesti -TM• Dudestii Vechi -TM• Horea - Ar• Siria - Ar• Recas - Ar• Semlac - Ar• Paulis - Ar• Barzava - Ar
Mn content in different wheat genotypes
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Fe content in different wheat genotypes
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Co content in different wheat genotypes
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Ni content in different wheat genotypes
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Cu content in different wheat genotypes
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Zn content in different wheat genotypes
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As content in different wheat genotypes
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Se content in different wheat genotypes
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Two countries, one goal, joint success!
Mo content in different wheat genotypes
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Cd content in different wheat genotypes
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Pb content in different wheat genotypes
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Pb content in different wheat genotypes
Summary
Maize genotypes:•Infections by all Fusarium strains increased the concentrations of microelements (Mn, Fe, Ni, Cu) and toxic metals (Cd, Pb) in the grains
Wheat genotypes:•Mn and Ni concentration were high in Barzava-Ar•Fe, Co, As content were higher in the chaff compared to grains in all genotypes•In the chaff of Horea-Ar high Pb and As concentrations were measured protection for the grain
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Two countries, one goal, joint success!
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