tracking year-to-year changes in intestinal nematode communities of rufous mouse lemurs
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Tracking year-to-year changes in intestinal nematode communities
Tuomas Aivelo,University of Helsinki
(@aivelo)
11.2.2015Oikos Finland
of rufous mouse lemurs
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Why longitudinal surveys?
Host individual is a dynamic habitat How parasite communities change? Understanding interactions in
parasite communities
→ virulence, prevalence etc.→ resistance, tolerance
Succession in parasite communities
→ host developmet
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Long-term studies are quite common, but...
Focus often on single species / taxons
Focus on component populations (= one parasite species in a host community)
Infrapopulations (= one parasite species in a single host) rarely studied Photo: St. Kilda Soay Sheep Project / Arpat Ozgul
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Research questions
Is there consistent succession in infracommunities?
How component and infracommunities relate to each other?
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Studying infracommunities
Mark-recapture of rufous mouse lemurs (Microcebus rufus)
3 years in Ranomafana National Park, in southeastern Madagascar
634 samples on 78 individual mouse lemurs
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...but it's not easy n thousand species of
nematodes which look the same
No adult specimens Identification of operational
taxonomic units Nematode barcoding routinely
with 18S gene Pyrosequencing to isolate and
sequence whole samples (1-1000 larvae
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2011vs.
2012
Putative species 1 and 2 quite stable
Highest diversity when the prevalence is the lowest
Putative species 1
Strongyloides
Putative species 2
Rhabditidae
Putative species 3
Strongylida
Putative species 4
Chromadorea
Putative species 5
Enterobius
Putative species 6
Panagrellus
Component communities
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First catches rarely contain nematodes
Rare putative species ephemeral
There's also turnover in common putative species
Infracommunities
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In nutshell
Component community quite stable, whereas pervasive variation in infracommunity
Role of transmission routes? Hibernation and parasites?
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What next?
Looking at the microbiome interaction between macrobiota and microbiota