topic 1 advances in sample prep ppt - agilent condition 1 ml methanol equilibrate 1 ml water wash 1...

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SampliQ SPE and QuEChERS Advancements

Agilent Technologies

Month ##, 200X

Agenda

• Agilent SPE Polymeric Phases

o Addition of 60 µm resin sizeo Stron Ion Exchange Polymeric Resins (SCX,SAX)o New Weak Ion Exchange Polymeric Resin (WCX,WAX)

• QuEChERSo Extensions of portfolioo Review of New Advancements in QuEChERS Applications

Month ##, 200XPage 2

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What is the SampliQ Polymer?Features:• High retention, outstanding recovery, and

excellent reproducibility• High sorbent robustness: if cartridges

accidentally go dry during the SPE process, you will not risk losing analytes and/or reproducibility

• No leaking bonded phases or other leachables that can contaminate valuable extracts

• Compatibility with most organic solvents and aqueous solutions over a pH range of 0 to 14

• Spherical particles and narrow size distribution, which ensure reproducible flow characteristics


Specifications:• spherical particles 25-35 µm• pore size 74-80Å using BET measure• pore size 1000Å using inverse size exclusion• surface area 475-505 m 2/g• particle quality controls

•Electrozone sensing (size)•Light microscopy (shape)•Mass Spectrometry (contaminants)

Resin Quality Controls :Packed Cartridge Test•Cartridge Purity Test (GC)•Frit Purity Test (GC)•Material Weight Check•Cartridge Flow Resistance•Extraction Residue (%)•Turbidity (NTU)

Electron Micrograph of the Polymeric Particles


• Spherical shape• Symmetrical• Easily packed• Higher capacity• No residual silanol

interactions• Better flow

characteristics

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SampliQ Polymer Line OPT and Strong Ion Exchange


O

R

N

R

R

x-Stream

R1

SO3-

R2 N

SCX Resin

R3

R2R1

SAX resin

Resin Resin

OPT • a novel polyamide polymer• has affinity for both hydrophobic and hydrophillic compounds

OPT

SCX• a sulfonic acid modified divinylbenzene polymer• has mixed-mode affinity for both basic and neutral compounds

SAX• a tertiary amine modified divinylbenzene polymer• has mixed-mode affinity for both acidic and neutral compounds

Polymer Performance is Robust

• Highly reproducible recoveries wet or dry

– Cartridges dried under vacuum for 10 minutes before the equilibration step • RSD’s of the recoveries for each of the compounds (n=5) very low • Compounds range from very polar, basic compounds to hydrophobic, neutral


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1acetaminophen

propranolol

brompheniramine

mianserin

doxepin

fluoxetine

Dihydroxynaphthalene

DRY DRY DRY DRY DRY DRY DRYWET WET WET WET WET WET WET

acetaminophen

propranolol

brompheniramine

mianserin

doxepin

fluoxetine

Dihydroxynaphthalene

DRY DRY DRY DRY DRY DRY DRYWET WET WET WET WET WET WET

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Extensions to SampliQ SPE Portfolio

•Why : To broaden the reach of our polymeric family

•What : The addition of weak ion exchange offers an extraction option of quaternary amines and strong acids, always charged compounds

•What : Addition of 60 micron size polymeric resins

•Why : Offer same extraction parameters of 30 micron, with increased throughput with greater flow characteristics


New SampliQ Polymeric SPE Products


Recently Released Polymeric SPE

Already on CPL

Newly Added

OPT SCX SAX WCX WAX OPT SCX SAX WCX WAX For Acids For Bases

1ml 30mg, 100/pk 5982-6013 5982-6213 5982-6313 5982-6513 5982-6613 5982-3013 5982-3213 5982-3313 5982-3513 5982-3613 5982-3713 5982-3813

3ml 60mg, 50/pk 5982-6036 5982-6236 5982-6336 5982-6536 5982-6636 5982-3036 5982-3236 5982-3336 5982-3536 5982-3636

6ml 150 mg, 30/pk 5982-6067 5982-6267 5982-6367 5982-6567 5982-6667 5982-3067 5982-3267 5982-3367 5982-3567 5982-3667

6ml 200 mg, 30/pk

6ml 500 mg, 30/pk 5982-6065

96 Well Plate 1ml/10mg 5982-3096

96 Well Plate 2ml/30mg 5982-6096 5982-6296 5982-6396 5982-6596 5982-6696 5982-3097 5982-3296 5982-3396 5982-3596 5982-3696

*Method Development 96 Well Plate = 3 rows each of SAX, SCX, WAX, WCX; Method Development Cartridge Kit = 10 ea. of SAX, WAX, and OPT (Acids); and SAX, WCX, and OPT (Bases)

5982-3796

Polymer Method Development Products*

Polymer

60 um 30um

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SampliQ WAX

•WAX (weak anion exchanger) resin is a neutral amine-modified divinylbenzene (DVB) polymer

•The polymeric resin exhibits retention for acidic/anionic, neutral and, more importantly, strong acids (e.g. sulfonates) that are irreversibly retained on strong anion exchange resins

•The amine functionality (pKa ~ 6) for the SampliQ WAX can be ionized or neutral (dependent on the pH of the buffer solution); unlike the strong anion exchange (SAX) resin (always ionized, pKa ~18) WAX offers a wider pH range

• The weak anion exchange resin exhibits both anion exchange and reversed phase behavior (mixed mechanism)


SampliQ WAX

•The weak anion exchange resin is inert to a wide variety of solvents, stable in the pH range 0 to 14, and is water-wettable

•The weak anion exchange resin’s pKa ~6


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Condition 1 mL Methanol

Equilibrate 1 mL Water

Wash 1 mL 25 mM Sodium Acetate, pH 4.5-5.0

Wash and Interference removal 1 mL Methanol

Dry 3 minutes

Dry 1 minute

Elute 2 x 1 mL 5% Ammonium Hydroxide/Methanol:ACN ( 20/80)

Load 1 mL sample (dilute 1:1 2% phosphoric acid in water pH 1.5-2.0)

Methanol fraction may be collected if basic and neutral compound capture is desired

Dry and reconstitute in mobile phase

Dry 1 minutes

SampliQ WAX ® Generic Method Process for Acidic/Anionic and Neutral Compounds


Performance of the Weak Anion Exchange Resin for the Extraction of Acidic/Anionic and Neutral Compounds from Human Plasma


0102030405060708090

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Exceptional Compound Recovery from SampliQ WAX

Acidic Compound Recovery from SampliQ WAX

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SampliQ WCX

•WCX (weak cation exchanger) is a neutral carboxylic acid-modified divinylbenzene (DVB) polymer

•The resin exhibits retention for basic/cationic, neutral and more importantly strong bases (quaternary amines) that are irreversibly retained on strong cation exchange resins

•The carboxyl functionality (pKa ~5) of the WCX resin can be ionized or neutral dependent on the pH of the buffer solution; unlike the strong cation exchange resin that is always ionized at all pH values, the WCX has a wider pH range.

•The WCX exhibits both cation exchange and reversed phase behavior (mixed mechanism)


SampliQ WCX

•The weak cation exchange resin is inert to a wide range of solvents, stable in the pH range 0 to 14, and is water-wettable


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SampliQ-WCX ® Generic Method Process for Strongly Basic/Cationic and Neutral Compounds


Condition 3 mL methanol

Equilibrate 3 mL water

Wash 1 mL 25 mM phosphate buffer (pH 7)

Interference removal: 2 mL methanol

Dry 1 minutes

Dry <2 minute

Elute with 5% formic acid in methanol:ACN (60/40)

Recommended flow through cartridge: not faster than 1mL per minute

Load 1 mL prepared sample in 2% phosphoric acidspiked with internal standard

Methanol fraction may be collected if neutral compound capture is desired

Dry and reconstitute in mobile phase

Dry 3 minutes

Performance of the Weak Cation Exchange Resin for the Extraction of Strongly Basic/Cationic and Neutral Compounds


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Atenolol Protryptyline Chlorpromazine

Basic Compounds Eluted by WCX

Basic Compounds

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Load:

Wash:

Elute 1:

Elute 2:

Equilibrate:

Condition:

Strong AcidspKa <1

Weak BasespKa 2-10

Weak AcidspKa 2-8

Strong BasespKa >10

Agilent SampliQ Polymeric A/N/B MethodNeutral

CompoundspKa 3-10

Weak Bases

Strong Acids

Strong Bases

Weak Acids

0.1% Formic Acid in Methanol

2% Formic Acid in Water

Prepared sample

2% Formic Acid

Methanol

Water

Prepared sample

5% NH4OH

2% Formic Acid in Methanol

Analyte:

Methanol

Water

Prepared sample

5% - 10% Methanol in Water

Methanol or 0.1% Formic Acid in

Methanol

Use: SampliQ OPT

Use: SampliQ SCX

Use: SampliQ WAX

Use: SampliQ SAX

Use: SampliQ WCX

Prepare Sample for SPE

Log P < 1.5 Log P < 1.5Log P > 1.3

Neutrals

5% NH4OH in Methanol

Weak Acids

100% Methanol

Weak Bases

100% Methanol


Method Development: Tips and Tricks

•Identify the compounds you need to extract

• pKa, Log P and matrix: biological, food, environmental

• Analysis by HPLC will offer insight into possible SPE modes

• The more details available will facilitate quick method development

•http://www.acdlabs.com/products/phys_chem_lab/logp/

•http://www.organic-chemistry.org/prog/peo/

•http://www.chemaxon.com/marvin/sketch/index.jsp

•http://www.pdfgeni.com/book/PKA-pdf.html


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Agenda

Traditional Sample Preparation Methods

What is QuEChERS?

How to choose the Right Kits?

Advantages of QuEChERS over Standard SPE

Applications


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Agenda


What is QuEChERS?



Applications


Traditional sample Preparation Methods- SPE


Prewash*

Remove contaminants that could elute with analyte

Precondition Load Wash Elute

Prepare cartridge to accept sample

Load sample and rinse reservoir(s)

Wash with solvent that won’t elute analyte

Elute analyte in smallest volume possible

If elution solventwill be stronger

than precond. solvent

1. MeOH or ACN

2. Weak solvent(water, buffer)

Weakly retained matrix compds

elute

Analyte and othermatrix compds

retained

Elute analyteleaving highly

retained compds

1 2

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Traditional sample Preparation Methods- GPC with Polymer or Silica Bases Columns


Zorbax PSM 60 S for GPC clean-upEPA test mixture2 x 250 mm L x 6.2 mm i.d. x 5 µm 60 A column combination1 mL/min MTBE/cyclohexane (50/50)

Agenda


What is QuEChERS?



Applications


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What is QuEChERS (pronounced “ Catchers ”)

• Quick, Easy, Cheap, Effective, Robust and Safe

•Sample Preparation Method targeting the Food Safety markets

• Developed jointly by US FDA and EU Food Regulatory Agencies

• Methodology for simplified extraction of a large number of pesticide residues in fruits and vegetables

Target Market - Food Safety scientists working with the analysis of pesticide/herbicide residues from fruits and vegetables

– Government inspection/regulatory agencies

– Food processing companies

– University/Academic researchers


Why is QuEChERS getting so important?

•Majority of current methods for pesticides in food use solid phase extraction (SPE)

•SPE requires multiple methods for specific classes of compounds

•A single QuEChERS method can be used to extract +250 compounds at one time

•After cleanup, can use extract for GC/MS or LC/MS analysis

Reduced solvent, reduced labor, increased lab productivity


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QuEChERS First Step - Extraction


QuEChERS Second Step – Dispersive SPEin less than 20 min


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Four Variations of the QuEChERS Method

�The original QuEChERS method was introduced in 2003, uses sodium chloride to enhance extraction

�AOAC 2007.01 uses sodium acetate as a buffer replacing sodium chloride and 1% acetic acid in acetonitrile

�The dual phase variation introduces the use of PSA and GCB cartridge to remove high levels of chlorophyll and plant sterols in the final extract without the loss of planar pesticides (polar aromatics) using an acetone:toluene solvent blend (3:1)

�European version is similar to the AOAC method, except the extraction uses sodium chloride, sodium citrate dihydrate and disodium citrate sesquihydrate instead of sodium acetate, and acid is added at the very end.


Modifications to the Original Schenck Method- Buffered Extraction

�Ensure efficient extraction of pH dependent compounds and minimize degradation of base and acid labile pesticides

�Citrate Buffering for the µ-extraction at a compromise pH of 5-5.5, most acid / base compounds in the sample extracts, small amount of formic acid is added to the final extract after cleanup

�Acidic pesticides are directly analyzed from raw extract before PSA cleanup since they would be absorbed and not released by the sorbent

�Graphitized carbon black (GCB) PSA cartridge is used to remove plant pigments without the loss of planar compounds


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Agenda


What is QuEChERS?



Applications


SampliQ QuEChERS Kits Making it Easy to Select the Right Kit

Choose your Extraction Kit based on the QuEChERS method you’re using, and the pesticides you’re screening for

– All salts and sorbents are pre-measured according to the specific method you are using.

Choose the Dispersive SPE Kit based on the type of food you’re analyzing and the method you are following

– A look-up reference of foods and kits can be found at www.agilent.com/chem/quechers


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SampliQ QuEChERS Selection Guide


Extraction SPE kits


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Extraction SPE kits



Dispersive SPE kits

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Dispersive SPE kits( contd)

New !!

SampliQ QuEChERS Kits Making it Easy to Select the Right Kit Provides food testing labs with a simplified approa ch to preparing samples for pesticide analysis

•No guesswork, no measuring: Pre-packed Extraction Kits and Dispersive SPE Kits are assembled to suit specific food types and screening protocols

•Extraction salts are pre-weighed and provided in anhydrous packets, so you can conveniently add them at the correct time and improve results by reducing analyte degradation

•Sorbents and salts for the dispersive SPE step are supplied in 2 mL or 15 mL centrifuge tubes, for 1 mL, 6 mL or 8 mL aliquot volumes, as specified by the various QuEChERS methodologies in use today

•Agilent’s tight quality control processes ensure that all QuEChERS salts, sorbents and supplies are high quality, and free of contaminants, so results are accurate and reproducible


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What Makes SampliQ QuEChERS Kits Unique?

• SampliQ QuEChERS kits have strong product advantages:

– Ease of selection: outline how to choose products based on analysis, regulatory method or sample size

– Pre-measured packets: provided in anhydrous packs ensure procedure is correctly followed, resulting in more accurate data

– Agilent provide ceramic homogeneizers to improve the recoveries

– Agilent provide standards

– Agilent focus on education: providing video

– Recognized brand, quality and WW technical support


The value of the kits•Individually packaged extraction salts improves acc uracy of results

– Vacuum-sealed anhydrous foil packs ensure salt integrity

– Allows the user to add the extraction salts as specified in the approved methods to avoid exothermic reactions

– Exothermic reactions occur in competitor’s kits when the sample is added directly to the salts already in tubes degrading analytes and decreasing recoveries

•No need for additional centrifuge tubes

– Less total cost

– Less waste (green solution)

•Agilent design innovations

– Convenient finger-tip access to all tubes

– Minimizes handling of tubes and promotes GLP

– Effortlessly determine remaining quantity at a glance

– Stores easily on shelf or bench

• No amorphous, plastic bags to shove in a drawer


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Cost Savings with Pre-made QuEChERS Kits


Cost / 100 samples

weigh in the SPE material needed incl. salt etc. for 100 samples 30 min x 60 €/h

30 €

PSA 25 g = 62 samples 59 € 95.16 €C18 EC 25g = 62 samples 59 € 95.16 €MgSO4 100g = 83 samples 33€ 39.76 €15 ml PP Centrifuge tubes 500 = 28€ 5.60 €total cost for 100 samples € 265.68Agilent QuEChERS Kit per sample 5982-5158for 50 samples incl. 15ml tubes = 106 €

€ 212 20% less

Ceramic Homogenizers for the Extraction step


• Extraction step requires the shaking of extraction salts with comminuted fruit/vegetable

• Consistency in shaking can be an issue, effecting recoveries and pesticide analysis• Limited Consistency in QuEChERS applications

Agilent’s Response: SampliQ’s CH: Ceramic Homogenizers• Offers more consistent extraction of the sample with the salts• Breaks up salt agglomerates • Facilitates homogenation by angle cut• Increase recovery of pesticides from sample• Also available in dispersive sizes too, 2 mL and 15 mL

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Ceramic Homogenizers improve recoveries


• Are made from a mixture of ceramic and alumina oxide• Are inert, showing no loss in pesticide recovery

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Reco

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Comparison of QuEChERS Method AOAC and EN With and Without Ceramic Homogenizers

AOAC Method with Ceramic HomogenizersEN Method with Ceramic HomogenizersAOAC Method without Ceramic HomogenizersEN Method without ceramic Homogenizers

Ceramic Homogenizers decrease shaking time


Average recoveries of several pesticide types with Ceramic Homogenizers

20 seconds of shaking with the ceramic homogenizers give equivalent or better recoveries than 1 minute shaking without them.

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Standards For QuEChERS:


QuEChERS Standards Part Number

HPLC & GC Internal Standard, AOAC Method 5190-0502

QC Solution, AOAC Method 5190-0503

HPLC Internal Standard, EN Method 5190-0500

GC Internal Standard, EN Method 5190-0501

QC Surrogate for GC Standard, EN Method 5190-0499

HPLC Standard Mix, EN Method 5190-0498

GC Standard Mix, EN Method 5190-0497

LC 6 Pt. Calib. Curve Test Kit EN Method 5190-0495

GC 6 Pt. Calib. Curve Test Kit EN Method 5190-0496

Why QuEChERS Standards:


• Ready to use for QuEChERS extractions-no dilution necessary

• Support GC and HPLC with MS, MS/MS, and selective detectors

• Takes all variability out of making standards and calibration curves, CRM (certified reference material), manufactured in accordance with ISO 9001, concentrations verified by ISO 17025 accredited laboratory, calibrated balances and glassware, weights traceable to NIST in compliance with ANSI/NCSL Z-540-1

• Convenient storage and usage in sealed amber ampoules, lot number and expiration date

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Agenda


What is QuEChERS?



Applications


Traditional Methods

Blending

Filtration

Large solvent volumes

Multiple partitioning steps

Transfers of entire extract

Lots of glassware

Evaporation Concentration

Classical SPE

Shaking

Centrifugation

Small solvent volumes

Single partitioning step

Take aliquots (use I.S.)

Single vessel

Large volume injection

Dispersive SPE

Advantages: QuEChERS vs. Standard Sample Prep


QuEChERS

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Advantages of Dispersive SPE Over Standard SPE


Dispersive SPE =

No SPE Apparatus No Flow Control needed

No SPE Cartridges No Elution Solvent

No Vacuum No Dilution of Extract

No pretreatment No Solvent Evaporation

No Channeling Less Sorbent

No Drying Out Less Time

No Collection Less Cost

Advantages of Dispersive SPE Over Standard SPE


Standard SPE method QuEChERS Method

Sample Processing: 120 min. 30 min. 25% of the time

Solvent usage: 60-90 ml Solvent usage: 10-15 ml

Chlorinated Solvents: 20-30 ml Chlorinated Solvents: None

Save 65%-92% of solvent Reduce Hazardous Waste by 56-87%Recoveries typically > 90% *

* A Comparison of Solid Phase A Comparison of Solid Phase Extraction Cleanups for use with the Extraction Cleanups for use with the QuEChERS Method QuEChERS MethodFRANK J. SCHENCK Southeast Regional Laboratory, FDA, Atlanta, GA Southeast Regional Laboratory, FDA, Atlanta, GAJon Wong & Michael Hennessy Jon Wong & Michael HennessyCFSAN, FDA, College Park, MD CFSAN, FDA, College Park, MD

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QuEChERS Advantages

�� A batch of 6-12 extracts can be prepared in 30-40 m in by a single analyst with ≈$1-3 of disposable materials per sample and generate <12 mL solvent waste and only a single re-usable item (FEP tube) for cleaning.

� Consistently high recoveries (mostly 90-110% with R SDs < 5%) of a wide range of GC- and LC-amenable pesticide s are achieved from many matrices.

� A low cost, highly effective sample preparation tec hnique which can be used on most food matricies for a wide range of pesticide classes


Agenda


What is QuEChERS?



Applications


27


Applications

Analysis of Pesticide Residues in Spinach Using Agi lentSampliQ QuEChERS AOAC Kits by GC/MS (Publication 5990-4305EN)

This application note describes the use of kits QuEChERS AOAC sample preparation approach for extraction and cleanup of 18 GC-amenable multiple pesticide class residues in spinach

In order to address the significant loss of planar pesticides caused by graphitized carbon black (GCB) in dispersive SPE, a modified method with addition of toluene was employed for the planar pesticides.

The target pesticides in the spinach extracts were then analyzed by gas chromatography/mass spectrometry (GC/MS) operating in selective ion monitoring (SIM) mode


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GC/MS of a Spinach Extraction by QuEChERS


GC/MS of a Spinach Extraction by QuEChERS


50 ng/g Fortified Spinach

Blank

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Results original method without toluene


Results original method with toluene


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Current Trends in QuEChERS Research

•In general the QuEChERS methodology is expanding out into others areas

• Drug Residue Analysis: Pharmaceuticals in Whole Blood

• Anthelmintics in Animal Tissue

• Veterinary Drugs in Animal Tissue*


Expanding the QuEChERS ConceptVeterinary Drugs in Animal Tissue

A method for the determination of 11 Quinolone anti biotics in bovine liver has been established:

• Analytes were extracted and cleaned up from bovine liver with Agilent SampliQ QuEChERS kits.

• Extraction was performed using SampliQ EN extraction kits and 5% FA in Acetonitrile.

• Clean up was performed using SampliQ dispersive SPE kits (25 mg C18 and 150 mg MgSO4), for drug residue in animal tissue (NEW)

• Extracted samples were then analyzed by LC/MS/MS

• Limits of Quantitation (LOQ) were 5.0 ng/g

• Calibration curves were linear over the range of 5.0 to 400 ng/g

• The sample pre-fortified recoveries were between 62.0% and 113.1% with RSD (n=6) values between 2.2% and 13.4%


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Weigh 2 g homogenized liver sample ( ±0.05g) in 50 mL centrifuge tube

Spike 100 µL of IS spike solution, 50 µL of QC spik e solution if necessary vortex 30 sec

Centrifuge @ 4000rpm for 5min

Transfer 1 mL of upper ACN layer to SampliQ QuEChER S dispersive-SPE 2 mL tube

Vortex 1min, centrifuge @ 13000rpm for 3 min with m icro-centrifuge

Add 10 mL of 5% FA in ACN, and shake vigorously for 30 sec

Add SampliQ EN QuEChERS extraction kit, and shake v igorously for 1 min

Transfer 800 µL extract to another tube, blow down @ 40°C with N 2

Sample are ready for LC/MS/MS analysis

QuEChERS Extraction Procedure for Bovine Liver


Add 8 mL of 30 mM KH 2PO4, pH 7.0 buffer, vortex

Reconstitute into 800 µL 1:9 MeOH/H 2O w/ 0.1% FA, vortex and sonicate

Filter samples w/ 0.22 µm Cellulose Acetate Spin Fi lter

LC-MS/MS Beef Liver Blank and 200 ng/g Fortified L iver ExtractsA

B

1

2

IS

3

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5

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7

8 9

10 11

LC/MS/MS Chromatograms of A) liver blank extract, and B) 200ng/g fortified liver extract. Peaks identification: 1. Pipemidic acid,2. Ofloxacin, 3. Ciprofloxacin, 4. Danofloxacin, 5. Lomefloxacin, 6. Enrofloxacin, 7. Sarafloxacin, 8. Cinoxacin, 9., Oxolinic acid, 10. Nalidixic acid, 11. Flumequine, IS (internal standard). Norfloxacin.


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LC-MS/MS Beef Liver


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in

Dan

oflo

xaci

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Lom

eflo

xaci

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oflo

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aflo

xaci

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Cin

oxac

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Oxo

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aci

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idix

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cid

Flu

meq

uine

Recovery and ReproducibilityR

ecov

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Instruments and Methods – LC/MS/MS

time (minutes) % B Flow Rate (mL/min)0 15 0.3

0.2 15 0.38.0 75 0.39.0 100 0.311.5 Stop


Chromatography System: Agilent 1200 HPLC System

HPLC conditions:• Column: Agilent Eclipse Solvent Saver Plus Phenyl-hexyl Column, 150×3.0mm 3.5µm (PN: 959963-312). • Flow rate: 0.3ml/min Column Temperature: 30oC • Injection volume: 10µl• Mobile phase: A) 5mM Ammonium Acetate in H2O, pH3.0, B) 1:1 Methanol/Acetonitrile.

• Gradient: Post Time: 4min

Mass Spectrometry System: Agilent 6410 Triple Quad Mass Spectrometrywith electrospray ionization.

Conditions: Polarity: Positive Gas Temperature: 325ºC Gas Flow: 8L/minNebulizer: 50psi Capillary : 4000VSolvent cut: 5min

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QuEChERS Advancements


� Data presented shows that the QuEChERS methodology can be used for other residues besides pesticides

� Very large scope of possibilities, with very simple sample preparation technique, “ just enough sample preparation ”

� Relatively green and very cost effective technique when compared to Liquid-Liquid Extraction and Solid Phase Extraction

QuEChERS – As a conclusion


� A ready to use and fast solution for pesticides analysis in Fruits and Vegetables

� Very effective, improve cost and time savings : improve the laboratory productivity!

� SampliQ QuEChERS Kits easy to choose, extract up to 250 pesticides in a row

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Additional Resources - QuEChERS

Check www.agilent.com/chem/quechers

Description of Extraction and Dispersive Kits

Description of QuEChERS SOP

Suggested SampliQ QuEChERS Dispersive Kits by Food Type and Method

Demo Video

Brochures

Application notes

Food Safety Notebook


topic 1 advances in sample prep ppt - agilent condition 1 ml methanol equilibrate 1 ml water wash 1...

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