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ML-00-00528Rev01 Questions about any product in our lineup? We’re just a click or phone call away. 800.526.5224 | [email protected] | diapharma.com Visit diapharma.com for our full line of products for TMA research, including anti-Factor H! *Research use only in the U.S. & Canada • Chromogenic Activity ELISA • Fluorogenic Activity ELISA • ADAMTS13 Antigen ELISA • ADAMTS13 Inhibitor ELISA • Automated ADAMTS13 on the Ceveron s100 • ADAMTS13 Screening Test Your partners for ADAMTS13 Research ADAMTS13 *Research use only in the U.S. & Canada ADAMTS13 FLUOROGENIC ELISA SCREENING ASSAY KIT AUTOMATED ADAMTS13 ASSAY TECHNOZYM ® ADAMTS13 Activity ELISA Prod. #5450701, 96 Tests TECHNOZYM ® ADAMTS13 Activity/Antigen ELISA Prod. #5450501, 2 x 48 Tests | Prod. #5450551, 48 Tests TECHNOSCREEN ® ADAMTS13 Activity Prod. #5700100, 10 Tests TECHNOFLUOR ® ADAMTS13 Activity Assay on CEVERON ® s100 Prod. #9822210, 1 Package TECHNOZYM ® ADAMTS13 INH ELISA Prod. #5450401, 96 Tests | Prod. #5450451, 48 Tests TECHNOZYM ® ADAMTS13 Antigen ELISA Prod. #5450601, 96 Tests 12 x 8 anti GST strips 1 x 12 ml HRP Conjugate 1 x 30 ml Reaction Buffer 1 x 53 ml Wash Buffer Concentrate 1 x 12 ml TMB Substrate 2 x 6 ml GST-vWF73 Substrate 1 x 12 ml Stop Solution 8 x 0.5 ml Calibrators and Controls 12/6 x 8 anti-ADAMTS13 strips 1 x 0.3 ml anti-ADAMTS13 Conjugate 1 x 80 ml Wash Buffer Concentrate 1 x 90 ml Incubation Buffer 2/1 x 3 ml Activity Substrate 1 x 6 ml Antigen Substrate 1 x 0.7 ml Stable Peroxidase Solution 1 x 6 ml Stop Solution 7 x 0.5 ml Calibrators and Controls 10 x test devices 10 x pre-incubation tubes 1 x 4 ml R1 1 x 1 ml R2 1 x 1 ml R3 11 x large pipettes (300 μl) 22 x small pipettes (60 μl) 1 x Short instruction pad 1 x Reference color chart 12 x 8 rec. ADAMTS13 strips 1 x 0.3 ml anti-IgG Conjugate 1 x 80 ml Wash Buffer Concentrate 1 x 90 ml Incubation Buffer 1 x 12 ml TMB Substrate 1 x 12 ml Stop Solution 7 x 0.5 ml Calibrators and Controls 12 x 8 anti ADAMTS13 strips 1 x 0.3 ml anti-ADAMTS13 Conjugate 1 x 80 ml Wash Buffer Concentrate 1 x 90 ml Incubation Buffer 1 x 12 ml TMB Substrate 1 x 12 ml Stop Solution 7 x 0.5 ml Calibrators and Controls ADAMTS13 CHROMOGENIC ELISAs Fully automated coagulation analyzer for clotting, chromogenic, turbidimetric assays, TGA and quenching assays.

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Page 1: ADAMTS13 · 12 x 8 rec. ADAMTS13 strips 1 x 80 ml Wash Buffer Concentrate 1 x 90 ml Incubation Buffer 1 x 12 ml TMB Substrate 1 x 12 ml Stop Solution 7 x 0.5 ml Calibrators and Controls

ML-00-00528Rev01

Questions about any product in our lineup? We’re just a click or phone call away.

800.526.5224 | [email protected] | diapharma.com

Visit diapharma.com for our full line of products for TMA research, including anti-Factor H!

*Research use only in the U.S. & Canada

• Chromogenic Activity ELISA

• Fluorogenic Activity ELISA

• ADAMTS13 Antigen ELISA

• ADAMTS13 Inhibitor ELISA

• Automated ADAMTS13 on the Ceveron s100

• ADAMTS13 Screening Test

Your partners for ADAMTS13 Research

ADAMTS13

*Research use only in the U.S. & Canada

ADAMTS13 FLUOROGENIC ELISA

SCREENING ASSAY KIT

AUTOMATED ADAMTS13 ASSAY

TECHNOZYM® ADAMTS13 Activity ELISA Prod. #5450701, 96 Tests

TECHNOZYM® ADAMTS13 Activity/Antigen ELISA Prod. #5450501, 2 x 48 Tests | Prod. #5450551, 48 Tests

TECHNOSCREEN® ADAMTS13 Activity Prod. #5700100, 10 Tests

TECHNOFLUOR® ADAMTS13 Activity Assay on CEVERON® s100 Prod. #9822210, 1 Package

TECHNOZYM® ADAMTS13 INH ELISA Prod. #5450401, 96 Tests | Prod. #5450451, 48 Tests

TECHNOZYM® ADAMTS13 Antigen ELISA Prod. #5450601, 96 Tests

12 x 8 anti GST strips

1 x 12 ml HRP Conjugate

1 x 30 ml Reaction Buffer

1 x 53 ml Wash Buffer Concentrate

1 x 12 ml TMB Substrate

2 x 6 ml GST-vWF73 Substrate

1 x 12 ml Stop Solution

8 x 0.5 ml Calibrators and Controls

12/6 x 8 anti-ADAMTS13 strips

1 x 0.3 ml anti-ADAMTS13 Conjugate

1 x 80 ml Wash Buffer Concentrate

1 x 90 ml Incubation Buffer

2/1 x 3 ml Activity Substrate

1 x 6 ml Antigen Substrate

1 x 0.7 ml Stable Peroxidase Solution

1 x 6 ml Stop Solution

7 x 0.5 ml Calibrators and Controls

10 x test devices

10 x pre-incubation tubes

1 x 4 ml R1

1 x 1 ml R2

1 x 1 ml R3

11 x large pipettes (300 μl)

22 x small pipettes (60 μl)

1 x Short instruction pad

1 x Reference color chart

12 x 8 rec. ADAMTS13 strips

1 x 0.3 ml anti-IgG Conjugate

1 x 80 ml Wash Buffer Concentrate

1 x 90 ml Incubation Buffer

1 x 12 ml TMB Substrate

1 x 12 ml Stop Solution

7 x 0.5 ml Calibrators and Controls

12 x 8 anti ADAMTS13 strips

1 x 0.3 ml anti-ADAMTS13 Conjugate

1 x 80 ml Wash Buffer Concentrate

1 x 90 ml Incubation Buffer

1 x 12 ml TMB Substrate

1 x 12 ml Stop Solution

7 x 0.5 ml Calibrators and Controls

ADAMTS13 CHROMOGENIC ELISAs

Fully automated coagulation analyzer for clotting, chromogenic,

turbidimetric assays, TGA and quenching assays.

Page 2: ADAMTS13 · 12 x 8 rec. ADAMTS13 strips 1 x 80 ml Wash Buffer Concentrate 1 x 90 ml Incubation Buffer 1 x 12 ml TMB Substrate 1 x 12 ml Stop Solution 7 x 0.5 ml Calibrators and Controls

ADAMTS13

ADAMTS13 Function

A Disintegrin and Metalloprotease with ThromboSpondin Motifs Chromogenic ELISA for the determination of ADAMTS13 activity

Test Principle

Correlation

Intact vessel wall ADAMTS13 regulates under normal circumstances the size of vWF multimers

Injured/inflamed vessel wall vWF multimers build a connection between collagen and platelets

Good correlation in ADAMTS13 activity of different groups of samples

Absence of ADAMTS13 Formation of very large vWF multimers leads to platelet aggregation in healthy vessels –> TTP

*Research use only in the U.S. & Canada *Research use only in the U.S. & Canada

Background:

• ADAMTS13 is a plasma protein with low concentration (5 nmol/L) which cleaves very specific large vWF multimeres under laminar flow conditions.

• A functional defect of ADAMTS13 leads to presence of higher molecular weight forms of vWF in plasma and thus to increased platelet aggregation.

• High sensitivity for ADAMTS13 activity down to 0.01 IU/ml

• Short assay time (3 h)

• Calibrators and Controls are traceable to WHO 1st international Standard ADAMTS13 plasma (NIBSC code 12/252)

• No interference by bilirubin, hemolytic or lipemic samples

• High precision with intra-assay CVs <5% and inter-assay CVs <9%

• Can be used for ADAMTS13 Inhibitor determination in Bethesda assay

Main cause for thrombotic thrombocytopenic purpura (TTP):

• Thrombotic microangiopathy (TMA) is a pathologic state which results in thrombosis in capillaries and arterioles, due to an endothelial injury. The classic TMAs are atypical hemolytic uremic syndrome (aHUS) and TTP.

• TTP occurs at very low ADAMTS13 activity concentration.

Presentation Dr. Scheiflinger, Laibach June 2004

TECHNOZYM® ADAMTS13 ACTIVITY (CHROMOGENIC) #5450701

Page 3: ADAMTS13 · 12 x 8 rec. ADAMTS13 strips 1 x 80 ml Wash Buffer Concentrate 1 x 90 ml Incubation Buffer 1 x 12 ml TMB Substrate 1 x 12 ml Stop Solution 7 x 0.5 ml Calibrators and Controls

TECHNOSCREEN® ADAMTS13 ACTIVITY

Semi-quantitative flow through assay for the determination of ADAMTS13 activity levels

Quick & Easy

*Research use only in the U.S. & Canada

The TECHNOSCREEN® ADAMTS13 is a semiquantitative flow through assay for the determination of ADAMTS13 activity levels in human citrated plasma. This assay is intended as a screening tool for estimating ADAMTS13 activity. Useful for selecting plasmas for ADAMTS13 research.

Test plasma is incubated with a vWF fragment. ADAMTS13 specific cleavage of the vWF is then detected with a cleavage site specific antibody by the amount of color development. The amount of cleavage directly correlates to the ADAMTS13 activity level in the plasma sample.

• Short instruction with a pre-incubation tube holder included in every package

• Large and small pipettes for the pre-incubation mixture included

• Easy test procedure for rapid results with ready to use reagents

• No additional instrument required

• Results in 30 minutes

• For the interpretation of the results a color chart is included in the kit

#5700100

*Research use only in the U.S. & Canada

Page 4: ADAMTS13 · 12 x 8 rec. ADAMTS13 strips 1 x 80 ml Wash Buffer Concentrate 1 x 90 ml Incubation Buffer 1 x 12 ml TMB Substrate 1 x 12 ml Stop Solution 7 x 0.5 ml Calibrators and Controls

TECHNOZYM® ADAMTS13 (FLUOROGENIC)

Fluorogenic ELISA for the determination of ADAMTS13 antigen and activity

Activity and Antigen Determination

*Research use only in the U.S. & Canada

• Fluorogenic substrate

• Incubation at room temperature

• Calculation tool available for antigen and activity

TECHNOZYM® ADAMTS13 ANTIGEN (CHROMOGENIC)

Chromogenic ELISA for determination of ADAMTS13 antigen concentration

• Measuring range 0.0 - 1.0 U/ml

• LOD 0.012 U/ml

• High linearity

• Calibrators and Controls are traceable to WHO 1st international Standard ADAMTS13 plasma (NIBSC code 12/252)

• Fast result within approximately 3.5 hours

• Detection is independent of enzyme activity

• Manual or automated test applications available

• Applications available for various fluorogenic readers

• No sample predillution

• Calibrators and Controls are traceable to WHO 1st international Standard ADAMTS13 plasma (NIBSC code 12/252)

TECHNOZYM® ADAMTS13 INHIBITOR (CHROMOGENIC)

Chromogenic ELISA for determination of antibodies directed against ADAMTS13

• Differentiation between TTP and non-TTP auto-antibodies

• Measuring range 2 - 104 U/ml

• LOD 1.68 U/ml

• High specifity

• Fast result within about 3 hours

• Manual or fully automated testing

TECHNOFLUOR® ADAMTS13 ACTIVITY ON CEVERON® s100

Time and Cost Saving

Easy and Fast

Quenching technology is based on high sensitive fluorescence measurement. Different ways of quenching methods, like dynamic quenching such as fluorescence resonance energy transfer (FRET), or static quenching which can be used.

The TECHNOFLUOR ADAMTS13 Activity test uses FRET technology. Due to the quenching reaction the donor fluorophore is activated, and transfers its energy to the quenching molecule which is emitting light. The measured fluorescence signal is proportional to the amount of VWF cleaved by ADAMTS13.

• Save valuable plasma and financial resources

• Fully automated ADAMTS13 activity result in less than 30 minutes

• Run ADAMTS13 activity sample as easily as a routine coagulation test

• Quenching module using parameters can be run in paralell to all other parameters including TGA

• Stable lot calibration curve with a high linearity

*Research use only in the U.S. & Canada

#5450501

#5450601

#5450451

#9822210

#5450401


“What is ADAMTS13 Anyway?” Maxwell Smith, MD August 19th, 2005

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