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Tissue Culture SolutionsFrom Whitehead Scientific (Pty) Ltd.

Products. Expertise. Support.

WhiteSciWhitehead Scientific (Pty) Ltd

ContentsCell Culture Dishes ..............................................................................................

Cell Culture Plates ...............................................................................................

Cell Culture Flasks ................................................................................................

Centrifuge Tubes .................................................................................................

Benchtop Storage Racks ....................................................................................

Serological Pipettes .............................................................................................

Cell Scraper .........................................................................................................

Syringe Filters ........................................................................................................

Cell Culture Classical Media ..............................................................................

Serum-free Media ................................................................................................

Mycoplasma Detection & Removal .................................................................

Cell Culture Reagents .........................................................................................

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Whitehead Scientific Tissue Culture Brochure Second Edition published August 2014

Whitehead Scientific is the proud supplier of Tissue Culture products from:

Quality Assurance:

PorvairProduction is carried out in a Class 100000 / ISO Class V clean room to ISO 9001:2008 and EN ISO 13485:2003 manufacturing standards to ensure all products are free from DNA/RNA, DNase/RNase and Pyrogen contamination.

LonzaThe Lonza sites are covered by a Global Certification Scheme, with a single ISO 9001:2008 Certificate for all certified sites.

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CERTIFIED NON-

PYROGENICRNaseFREEDNase/

TRACEABILITYLOT NUMBERISO

REGISTERED

9001

• High clarity, 100% virgin polystyrene• Vacuum plasma tissue culture treatment• Special vents for air exchange• Packaged in gamma irradiated sterile sleeves• Clear lot number for batch traceability• Non-pyrogenic, DNase/RNase free

• Flat bottom with low evaporation lid• Tissue culture treated plate• Polystyrene• Non-pyrogenic• Individually packaged• Sterile

Porvair tissue culture plates are packaged in paper peel pouches that are designed to keep the plates in pristine condition until use.

Cell Culture Plates

Porvair tissue culture dishes are ideal for all types of culturing where microscopic examination is required. Numeric indicators on the bottom of each culture dish allows users to identify the location of cells.

Cell Culture Dishes

Cat # Description Pack size500026 35mm diameter Cell Culture Dish, inner pack 20 x 25 500500025 60mm diameter Cell Culture Dish, inner pack 20 x 25 500500024 100mm diameter Cell Culture Dish, inner pack 20 x 15 300

Cat # Description Pack size500023 6-well Cell Culture Plate 50500022 12-well Cell Culture Plate 50500021 24-well Cell Culture Plate 50500020 96-well Cell Culture Plate 100

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Cell Culture Flasks

Porvair cell culture flasks have an even surface with unrivalled optical purity. Vacuum plasma tissue culture treatment ensures maximum and uniform cell growth, ensuring cells adhere more efficiently to the surface by reducing hydrophobicity. Vented and sealed caps are available for all sized flasks.

• Packaged in sterile, zip-sealable bags• Non-pyrogenic, DNase/RNase free• 100% USP class VI polystyrene, optically clear• Frosted writing and clear graduations• Notched bottom for slip free stacking• Opaque areas for laboratory markers• Moulded graduations in the flask walls• Sector division marks on the dishes• Ridge underneath neck of flask to prevent tipping forward

Porvair cell culture flasks feature:

Plug Seal Cap

Filter Vent Cap

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Cat # Description Pack size500030 25cm² Cell Culture Flask, 50ml volume, inner pack of 10 x 20 200500029 75cm² Cell Culture Flask, 250ml volume, inner pack of 5 x 20 100500028 175cm² Cell Culture Flask, 750ml volume, inner pack of 5 x 8 40

Cat # Description Pack size500033 25cm² Cell Culture Flask, 50ml volume, inner pack of 10 x 20 200500032 75cm² Cell Culture Flask, 250ml volume, inner pack of 5 x 20 100500031 175cm² Cell Culture Flask, 750ml volume, inner pack of 5 x 8 40

Racks

Bulk in bags

Self standing

Centrifuge Tubes

Benchtop Storage Racks

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Cat # Description Pack size500037 15ml Centrifuge Tubes bulk, bags of 50 x 10, sterile 500500036 50ml Centrifuge Tubes bulk, bags of 25 x 20, sterile 500

Cat # Description Pack size500039 50ml Centrifuge Tubes, resealable bags of 50 x 10, sterile 500

Cat # Description Pack size500035 15ml Centrifuge Tubes in racks of 50 x 10, sterile 500500034 50ml Centrifuge Tubes in racks of 25 x 20, sterile 500

The 4-way rack from MBP is the ultimate bench top storage system combining four great racks in one! Designed to support a variety of tube sizes and styles, each rack can be rotated to any of the four sides to support different size centrifuge tubes. Constructed of rugged polypropylene, these racks are autoclavable, may be placed in the freezer, or used in waterbaths. Regardless of the support position used, the format is small enough to be conveniently used under the hood or on the benchtop.

Cat # Colour Pack Size 8850 Fluorescent Green 1 8860 Fluorescent Orange 1 8870 Fluorescent Pink 1

Porvair standard centrifuge tubes are available in 15ml and 50ml sizes. The thin clear polypropylene walls can withstand centrifugal forces of 8000xG and 12000xG respectively.

Porvair self-standing centrifuge tubes are supplied in 50ml size. The skirted conical bottom allows the tube to stand on a bench and can withstand 6000xG.

• 4 x 50ml conical tubes• 12 x 15ml tubes• 32 x 1.5/2ml microcentrifuge tubes• 32 x 0.5ml tubes

This rack will hold either:

Serological Pipettes

• Suitable for most brands of pipette-aids• Colour-coded stripes for quick volume identification• Individually wrapped, paper peel• Calibrated for accurate dispensing to within ±2%• Made of the highest quality virgin crystal polystyrene• Non-pyrogenic, DNase/RNase free

All pipettes are supplied with a filter plug and are individually wrapped in sterile easy to open packs. The 10ml and 25ml pipettes have an ultrasonically welded mouth piece for extra strength and safety.

Porvair serological pipettes are high-quality disposable pipettes that can be used for a variety of applications.

The range of disposable, colour-coded, Porvair pipettes covers all the popular sizes from 1ml to 25ml.

Cat # Description Pack size500050 1ml Serological Pipette, single-piece extrusion 500500051 2ml Serological Pipette, single-piece extrusion 500500052 5ml Serological Pipette, single-piece extrusion 200500053 10ml Serological Pipette, ultrasonically welded 200500055 25ml Serological Pipette, ultrasonically welded 150

• Two-position charging stand• Ergonomic design for exceptional comfort• Large LCD display for continuous visualisation of speed, modes and battery status• Easy access to battery compartment• Easy maintenance

Cat# P2002

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Cell Scraper

The Porvair cell scraper is specially designed to make the process of scraping off and collecting cells easy and effective.

• 25cm total length and 2cm blade length• Two blade positions: scraper and lifter• Sterilised by Gamma irradiation• Individually wrapped• Non-pyrogenic

Syringe FiltersSyringe/vacuum-driven filters are an essential way to purify cell culture media and supplements. Porvair offers these with three popular membranes:

Polyethersulfone (PES) has a low affinity for proteins and extractables; suitable for pre-filtration and filtration of buffers and culture media.

Mixed Cellulose Ester (MCE) is ideal for filtration of aqueous solutions; effectively binds trace proteins.

Nylon provides a broad range of chemical compatibility for the filtra-tion of either aqueous or organic solvents. It is hydrophobic and can be used in a broad pH range.

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Cat # Description Pack size500062 Syringe Filter, 30mm dia. PES 0.22μm 45500063 Syringe Filter, 30mm dia. MCE 0.22μm 45500064 Syringe Filter, 30mm dia. Nylon 0.45μm 45

Cat # Description Pack size500065 Cell Scraper, 25cm total length and 2cm blade length 100

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Feature Nucleic acid

Benefit for transfection

Cell lines

Transfection in presence of serumReverse transfectionTested for endotoxins

HiPerFectsiRNA, miRNA

Low siRNA amounts minimise off-target effects

Eukaryotic cell lines and primary cellsYes

Yes-

HiPerFect HTSsiRNA, miRNA

Easy to use andeconomical forhigh-throughputexperimentsEukaryotic cell lines and primary cellsYes

Yes-

AttracteneDNA, siRNA, or miRNA/DNAcotransfectionEfficient transfection with very low cytotoxicityAdherent cells

Yes

Yes-

EffecteneDNA

Highly effective for primary and sensitive cells

Eukaryotic cell lines and primary cellsYes

YesYes

EffectenemRNA

Efficient transfection

Eukaryotic cell lines and primary cellsYes

YesYes

QIAGEN siRNAFlexiTube siRNA and FlexiTube GeneSolution

siRNA in convenient 1 nmol, 5 nmol, and 20 nmol amounts

• Cost-effective siRNA enables analysis of more genes• Optimal solutions for every gene• Innovative design minimises the risk of off-target effects• Easy to search for and order siRNA at GeneGlobe (www.qiagen.com/GeneGlobe)• Thousands of human siRNAs have been experimentally verified

E�cient and robusttransfection – for allyour applications

QIAGEN Chemical Transfectants

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Lonza Clonetics® Primary Cells and Media

The 4D-Nucleofector™ System is a modular system comprising one core unit and up to 5 different functional units.

• Easy – Novel technology for transfection of various cell numbers with same conditions at different throughput• Fast – Different throughput, from 1 - 16 wells in 10 seconds• Flexible – Nucleofection® of cells in adherence for functional assays at various cellular stages• Future proof – Modular system for upcoming transfection challenges

For primary cells or difficult to transfect cell lines.

Easy. Fast. Flexible. Future proof

Stretching Transfection DimensionsLonza 4D-Nucleofector™ System

• Normal human and animal primary derived cells – biologically more relevant• Cryopreserved cells – ready for use when you are• Proliferation and/or differentiation media – optimised so you spend time getting results• Guaranteed system performance – consistent, reproducible quality every time

Primary cells and related products are known to provide more biologically relevant results than cell lines. Primary cells are non-transformed, non-immortalised cells isolated directly from tissue, and provide conditions that closely simulate a living model and yield more physiologically significant results.

Cell Culture Classical MediaCell culture involves the placement of cells, tissues or organs into an artificial envi-ronment conducive for growth. The most crucial step in cell culture is selecting an appropriate growth medium for in vitro cultivation. Liquid media provides the nutrients essential to growth, together with buffers to maintain pH and osmolality in the culture system.

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Cat # Description SizeBE04-649F Grace’s insect media with lactalbumin, yeastolate, gentamicin 500ml 09-774F RPMI 1640 with HEPES, Pen/Strep and L-Glutamine 500ml BE12-115F/12-115F RPMI 1640 with HEPES and L-Glutamine 500ml BE12-117F/12-117F Medium 199 with EBSS, with L-Glutamine and HEPES 500ml 12-132A Cryoprotective media without L-Glutamine, with 15% DMSO 500ml BE12-136F/12-136F MEM Eagles with Earle’s BSS, without L-Glutamine, with HEPES 500ml BE12-167F/12-167F RPMI 1640 without L-Glutamine 500ml BE12-168F/12-168F McCoy's 5 A with L-Glutamine and HEPES 500ml BE12-604F/12-604F DMEM 4.5 g/l glucose, with L-Glutamine 500ml BE12-611F/12-611F MEM Eagles with Earle’s BSS with L-Glutamine 500ml BE12-614F/12-614F DMEM 4.5 g/l glucose, without L-Glutamine 500ml BE12-615F/12-615F Ham’s F12 with L-Glutamine 500ml BE12-618F/12-618F Ham’s F10 with L-Glutamine 500ml BE12-662F/12-662F MEM Eagle EBSS with NEAA, Pyr, without L-Glutamine 500ml BE12-702F/12-702F RPMI 1640 with L-Glutamine 500ml BE12-719F/12-719F DMEM F12 1:1 mix, with HEPES and L-Glutamine 500ml BE12-914F/12-914F DMEM 4.5 g/L glucose without L-Glutamine and without Phenol red 500ml BE12-917F/12-917F DMEM 4.5 g/L glucose without L-Glutamine 500ml BE12-918F/12-918F RPMI 1640 Medium without L-Glutamine and without Phenol red 500ml BE02-011F TC 100 Insect Medium 500ml BE12-604F/U1 DMEM with UltraGlutamine 1 500ml

• Strictly manufactured and quality assessed to ensure lot-to- lot consistency (in accordance with cGMP guidelines) • Manufactured using Water For Injection (WFI) quality water • Sterile filtered • Formulated to guidelines recommended by the Tissue Culture Association

All BioWhittaker™ Classical Media products are:

Serum-Free Media

Mycoplasma Detection & Removal

Serum-free media and reagents have a wide range of applications, including production of monoclonal antibodies, viral antigens, and recombinant proteins using a variety of mammalian and invertebrate cell lines. There are numerous advantages associated with the use of serum-free media formulations.

Lonza provides a powerful product for reliable detection, successful elimination and prevention of mycoplasma contaminations:

• Detection - easily detect mycoplasma using the luminescent MycoAlert™ PLUS Mycoplasma Detection Kit • Elimination - eliminate mycoplasma with the MycoZap™ Elimination Reagent• Prevention - prevent mycoplasma with our MycoZap™ Antibiotics or MycoZap™ Spray

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Cat # Description SizeLT07-118 MycoAlert® Mycoplasma detection kit 10T LT07-218 MycoAlert® Mycoplasma detection kit 25T LT07-318 MycoAlert® Mycoplasma detection kit 100T LT07-518 MycoAlert® Assay control set 10T LT37-618 MycoAlert® sample pack 5T

Cat # Description SizeBE12-730F/12-730F Insect-XPRESS with L-Glutamine 500ml BE12-730Q/12-730Q Insect-XPRESS with L-Glutamine 1L BE02-015E Lymphochrome serum free karyotyping medium 100ml BE12-756FCM Amniochrome II modified complete medium 500ml BE12-764Q/12-764Q Pro293 Chemically defined, serum-free media 1L

• Increased definition• Increased lot-to-lot consistency• Simplified purification and downstream processing• Better control over the physiological condition of cultures• Ability to optimise formulations for specific cell types

Benefits include:

Cell Culture Reagents

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Cat # Description Size09-757F Pen/Strep stock, 20K/20K 500ml BE10-508F/10-508F Hanks BSS(1X) with phenol red 500ml BE10-527F/10-527F Hank's BSS without phenol red 500ml BE10-543F/10-543F Hanks BSS without Ca++ Mg++ with phenol red 500ml BE10-547F/10-547F Hanks BSS without Ca++ Mg++ or phenol red 500ml BE13-114E/13-114E MEM Eagle non-essential amino acid solution 100ml BE13-115E/13-115E Sodium Pyruvate solution 100ml BE17-160E/17-160E Trypsin (10x) 2,5% in HBSS without Ca++ Mg++ 100ml BE17-161E/17-161E Trypsin-Versene (EDTA) 100ml BE17-512F/17-512F Dulbecco PBS (1x) without Ca++ Mg++ 500ml BE17-513F/17-513F DPBS (1X) with Ca++ Mg++ 500ml BE17-516F/17-516F PBS (1x) TC grade 500ml BE17-516Q/17-516Q PBS (1x) TC grade 1L BE17-517Q/17-517Q PBS (10x) TC grade 1L 17-518L Gentamicin 50mg/ml (screw cap) 10X10ml 17-518Z Gentamicin 50mg/ml (screw cap) 1x10ml 17-519L Gentamicin 10mg/ml (screw cap) 10x10ml 17-519Z Gentamicin 10mg/ml (screw cap) 1x10ml DE17-602E/17-602E Penicillin/Streptomycin mixture 10Kunits/10Kug 100ml BE17-605E/17-605E L-Glutamine 200mM 100ml BE17-724F/17-724F Water for cell culture 500ml BE17-724Q/17-724Q Water for cell culture 1L BE17-737E/17-737E HEPES Buffer in normal saline 100ml BE17-745E/17-745E Pen/Strep/Fungizone 100ml 17-829E Lymphocyte separation medium, density 1.077 100ml 17-836R Fungizone (Amphotericin B) 20ml 17-942E Trypan Blue 0,4% 100ml BE02-007E Trypsin/EDTA 10X 100ml

BioWhittaker™ cell culture reagents include a number of different products, such as amino acids, antibiotics and buffers, all of which are used routinely in applications involving cell culture. These products are manufactured under the same GMP con-ditions as our other cell culture products.

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• Rapid sample screening• Simple technology – easy to use• Large number of targets per array• No specialised equipment required• Choice of detection substrates

Proteome Profiler Antibody Arrays allow for the measure-ment of up to 119 proteins in a single sample. These arrays require no specialised equipment and eliminate the need for multiple Western blot experiments. Antibody array kits contain buffers, detection antibodies, and membranes spotted in duplicate with high quality capture antibodies. The arrays utilise chemiluminescence for detection and membranes can be assessed for protein levels in the same manner as traditional Western blots. Select arrays are also suitable for use with the LI-COR® detection system.

Proteome Profiler™ Antibody Arrays

See no evil

See no evil

See no evil See no evil See no evil

Hear no evilHear no evil

Hear no evil

Hear no evil

Hear no evilSpeak no evil

Speak no evilSpeak no evil

Hear no evil

When did you last test for mycoplasma?

Mycoplasma contaminations can seriously impact the reliability, reproducibility, and consistency of experimental results.

The MycoAlert™ PLUS Assay is a selective biochemical test that exploits the activity of mycoplasmal enzymes which are found in all six of the main mycoplasma cell culture contaminants and the vast majority of 180 mycoplasma species.

MycoAlert™ PLUS Mycoplasma Detection Kit

Detection Antibody

Streptavidin-HRP

Target Analyte

Capture Antibody

Array Membrane

Array Membrane

Substrate Light

Biotin

ASSAY PRINCIPLE

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Key Features• Configurable to different well formats for maximum flexibility• Optional 16-way manifold for washing 384-well plates• Detachable plate carrier for easy cleaning• Low noise, pressure-free aspiration and dispensing pumps• Auto rinse function prevents liquid path clogging• Reduces aerosols due to cover and pumping technology• Optional liquid level sensors

Key Features:• Easy to use• Create new plate layouts• Add multiple assays to a plate• Add multiple plates per analysis• Select only the data you want for data reporting• Windows 7 & 8 compatibility as well as older Windows OS from XP.

Everything you need to measure your ELISA assays:• Measures flat, round and v-bottomed 96-well microplates and stripwells with standard ANSI SBS footprints• Accurate measurements ensured by automatic lamp calibration prior to each reading• USB 2.0 PC connection

Biochrom EZ Read 400 ELISA Microplate Reader

EZ Read 400 ELISA and EZ Read software delivers everything you need to make your ELISAs easy...

Biochrom Asys Atlantis Microplate Washer

The Biochrom Asys Atlantis microplate washer for standard ANSI SBS footprint 96-well plates with flexiblility in washing options, simple operation and digitally controlled aspiration and dispensing pumps. The perfect choice to compliment any microplate reader in any applications environment.

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Santa Cruz BiotechnologyQuality antibodies and biochemicals

Santa Cruz Biotechnology is a world leader in the development of products for the biomedical research market, providing an extensive range of research antibodies,

reagents and biochemicals.

| Proteins | Antibodies | ELISAs | Multiplex Assays/Arrays | Activity Assays & Reagents | | Stem Cell & Cell Culture Products | ELISpot Kits and Development Modules | | Flow Cytometry & Cell Selection/Detection | General Laboratory Reagents |

R&D Systems –Tools for Cell BiologyR&D Systems offers a range of high quality tools for research in Cell Biology including:

Research Product Categories:Primary antibodies: mammalian

Phospho-specific antibodiesAntibodies for IHC (Paraffin) staining

Flow CytometryVeterinary research antibodies

Primary antibodies: non mammaliansiRNA and shRNA (plasmid and lenti virus) products

Proteins A, G PLUS L agaroseTissue extracts and cell lysates

Biologically active and purified proteinsTransfected cell lysates

Secondary antibodies, control antibodies and normal seraSpeciality reagents and kits

Chemicals

Santa Cruz Biotechnology, Inc. now offers over 140,000 specialty biochemicals under the ChemCruz™ brand.

BiochemicalsStains, dyes and fluorescent probes

Whitehead Scientific (Pty) LtdSouth Africa - Cape Town(tel) +27 (0) 21 944 6460 - (fax) +27 (0) 21 949 5478PO Box 194, Brackenfell, 7561, Cape Town, South Africawhitesci@whitesci.co.za - www.whitesci.co.za

Whitehead Scientific is the proud supplier of Tissue Culture products from:

Products. Expertise. Support.

WhiteSciWhitehead Scientific (Pty) Ltd