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The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea Nitrogen Concentrations of Feedlot Steers Phase Fed Different Levels of Protein. L.W. Greene 1, 2 and J. T. Vasconcelos 1,3 1 Texas Agricultural Experiment Station - Amarillo 2 West Texas A&M University, Canyon 3 Texas A&M University

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Page 1: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

Manure Concentration of N:P, Animal Performance, and Blood Urea Nitrogen

Concentrations of Feedlot Steers Phase Fed Different Levels of Protein.

L.W. Greene1, 2 and J. T. Vasconcelos1,3

1Texas Agricultural Experiment Station - Amarillo2West Texas A&M University, Canyon3Texas A&M University

Page 2: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

Introduction• Cattle are started on high concentrate finish diets at

approximately 750 lbs and fed to an average weight of 1250 lbs.

• Early in the feeding period, cattle have a greater deposition of lean tissue and less deposition of fat compared to later in the feeding period.

• As animals mature through the feeding period, the composition of gain changes -- protein gain decreases and fat gain increases in relative proportion.

Page 3: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

Introduction

• The typical feedlot diet in the Southern Plains contains 13.0 to 13.5% Crude Protein (% N X 6.25)

Page 4: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

Days on Feed-400

-300

-200

-100

0

100

200

300

14 42 70 99 127

Crude protein excess or deficiency (g/d) during feeding period given a

typical diet (adapted from Galyean)

Days on Feed

Average excess = 50 g/d

Page 5: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

-10

0

10

20

30

40

1 2 3

14.5% CP13% CP

11.5% CP

Nitrogen retained, % of intake

Collection Period

%

Page 6: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

• The typical feedlot diet in the Southern Plains contains approximately 0.33% P

• The typical feedlot steer requirement for P is likely < 0.18%.

Introduction

Page 7: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

-4

-2

0

2

4

6

8

10

12

14

14 42 70 99 127

Phosphorus excess or deficiency (g/d) during feeding period given a typical

diet (adapted from Galyean)

Days on Feed

Average excess 6.7 g/d

Page 8: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

0

10

20

30

40

50

60

1 2 3

14.5% CP13% CP11.5% CP

Phosphorus retained, % of intake

Collection Period

%

Page 9: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

• The typical feedlot diet does not contain supplemented P.• The dietary P is supplied from the dietary ingredients.

Introduction

0.90+28.0Dried distillers grains

0.9346.0Cottonseed meal

0.3022.0Alfalfa hay

0.2511.0Sorghum silage

0.279.0Corn silage

0.319.8Corn grain

Phosphorus, %Crude Protein, %Source

Page 10: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

Objective• Determine the effects of phase-

feeding of protein in finishing steers on– Steer performance– Blood urea nitrogen concentration– Manure N concentration– Manure P concentration– Carcass characteristics

Page 11: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

Methods

One hundred eighty four steers

Randomized block design (blocked by weight into heavy medium and light and by start date)

54 steers started on June 6, 2003130 steers started on June 17, 2003

Steers were housed in 21 pens (7 pens/treatment)

Page 12: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

Methods Three dietary treatments

1. Control diet – 13% Crude protein diet fed continually throughout the feeding period

2. 11.5% Crude protein – 13% Crude protein diet fed until cattle the average of the block reached 477 kg, then switched to an 11.5% crude protein diet

3. 10.0% Crude protein – 13% Crude protein diet fed until cattle the average of the block reached 477 kg, then switched to no supplemental crude protein diet

Page 13: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

MethodsThe supplemental protein was supplied from equal amounts of nitrogen from urea and cottonseed meal

Page 14: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

MethodsIngredient composition of finishing diets (DM basis)Item 13.0% CP 11.5% CP 10.0% CP

Corn grain, Steam flaked, % 74.07 76.25 79.50

Sorghum-sudan silage, % 7.50 7.50 7.50

Molasses, cane, % 4.00 4.00 4.00

Fat, % 3.00 3.00 3.00

Limestone, % 1.00 1.00 1.00

Mineral and vitamin premix, % 5.00 5.00 5.00

Urea, % 0.75 0.46 -

Cottonseed meal, % 4.68 2.79 -

Page 15: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

Analyzed chemical composition of finishing diets.Item 13.0% CP 11.5% CP 10.0% CP

Crude protein, % 12.07 10.57 8.30

NDF, % 13.70 15.47 13.47

NEl, (Mcal/lb) 0.83 0.82 0.87

NEm, (Mcal/lb) 0.87 0.86 0.91

NEg, (Mcal/lb) 0.58 0.57 0.61

Ca, % 0.94 1.03 0.82

P, % 0.31 0.31 0.27

Methods

Page 16: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

2.0062.042 1.965

1

1.2

1.4

1.6

1.8

2

2.2

13.0%CP

11.5%CP

10.0%CP

kg/d

Average Daily GainBeginning until diet change

P-value =0.912

Page 17: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

1.622

1.713

1.526

11.11.21.31.41.51.61.71.8

13.0%CP

11.5%CP

10.0%CP

kg/d

Average Daily GainDiet change until finish

P-value =0.213

Page 18: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

1.743

1.8581.687

1

1.2

1.4

1.6

1.8

2

13.0%CP

11.5%CP

10.0%CP

kg/d

Average Daily GainBeginning until finish

P-value =0.094

Page 19: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

10.229 10.17910.328

5

6

7

8

9

10

11

13.0%CP

11.5%CP

10.0%CP

kg/d

Dry matter intakeBeginning until diet change

P-value =0.619

Page 20: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

11.077 10.777 10.079

56789

101112

13.0%CP

11.5%CP

10.0%CP

kg/d

Dry matter intakeDiet change until finish

P-value =0.008

Page 21: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

10.751 10.61 10.151

5

6

7

8

9

10

11

13.0%CP

11.5%CP

10.0%CP

kg/d

Dry matter intakeBeginning until finish

P-value =0.056

Page 22: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

0.19

0.201 0.196

0.1

0.12

0.14

0.16

0.18

0.2

13.0%CP

11.5%CP

10.0%CP

Kg gain/Kg intake

Gain efficiencyBeginning until diet change

P-value =0.832

Page 23: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

0.151

0.159 0.146

0.1

0.12

0.14

0.16

0.18

0.2

13.0%CP

11.5%CP

10.0%CP

Gain efficiencyDiet change until finish

P-value =0.294

Kg gain/Kg intake

Page 24: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

0.166

0.175 0.162

0.1

0.12

0.14

0.16

0.18

0.2

13.0%CP

11.5%CP

10.0%CP

Gain efficiencyBeginning until finish

P-value =0.094

Kg gain/Kg intake

Page 25: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

Plasma Urea Nitrogen (mg/dL)

7.72 7.31 6.52

1

6

11

P-value =0.8280

Day 0

6.56 6.94 6.12

1

6

11

P-value =0.8100

Diet change

9.847.02 4.75

1

6

11

P-value =0.0001

Harvest

Page 26: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

2.8

2.42

2.6

1

1.5

2

2.5

3

13.0%CP

11.5%CP

10.0%CP

Pen surface manure concentrationNitrogen P-value =0.600

%

Page 27: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

0.10.11

0.12

00.020.040.060.08

0.10.120.14

13.0%CP

11.5%CP

10.0%CP

Pen surface manure concentrationAmmonia Nitrogen P-value =0.931

%

Page 28: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

2.72

2.33

2.47

1

1.5

2

2.5

3

13.0%CP

11.5%CP

10.0%CP

Pen surface manure concentrationOrganic Nitrogen P-value =0.549

%

Page 29: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

0.780.72 0.68

0.40.45

0.50.55

0.60.65

0.70.75

0.8

13.0%CP

11.5%CP

10.0%CP

Pen surface manure concentrationPhosphorus P-value =0.642

%

Page 30: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

3.56 3.45

3.87

2

2.5

3

3.5

4

13.0%CP

11.5%CP

10.0%CP

Pen surface manure concentrationNitrogen:Phosphorus P-value =0.038

Page 31: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

1.15

1.281.15

0.50.60.70.80.9

11.11.21.3

13.0%CP

11.5%CP

10.0%CP

Carcass CharacteristicsFat thickness, cm P-value =0.227

Page 32: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

87.33 85.9487.33

505560657075808590

13.0%CP

11.5%CP

10.0%CP

Carcass CharacteristicsLongissimus area, cm2 P-value =0.192

Page 33: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

1.891.92

1.97

1

1.2

1.4

1.6

1.8

2

13.0%CP

11.5%CP

10.0%CP

Carcass CharacteristicsKidney, Pelvic and Heart Fat, % P-value =0.459

Page 34: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

360.1361.1

363.76

300310320330340350360370

13.0%CP

11.5%CP

10.0%CP

Carcass CharacteristicsHot carcass wt, kg P-value =0.663

Page 35: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

577579

580

500

520

540

560

580

13.0%CP

11.5%CP

10.0%CP

Carcass CharacteristicsLive weight, kg P-value =0.763

Page 36: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

62.8 62.7 62.28

58

59

60

61

62

63

64

13.0%CP

11.5%CP

10.0%CP

Carcass CharacteristicsDressing percentage, % P-value =0.874

Page 37: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

430.43 409.44

438.1

200

250

300

350

400

13.0%CP

11.5%CP

10.0%CP

Carcass CharacteristicsMarbling score P-value =0.173

Page 38: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

2.69

2.912.92

2

2.2

2.4

2.6

2.8

3

13.0%CP

11.5%CP

10.0%CP

Carcass CharacteristicsYield grade P-value =0.174

Page 39: The Texas A&M University System Agricultural Research and Extension Center at Amarillo Manure Concentration of N:P, Animal Performance, and Blood Urea

The Texas A&M University SystemThe Texas A&M University SystemAgricultural Research and Extension Center at AmarilloAgricultural Research and Extension Center at Amarillo

• Variation of crude protein in the experimental diet resulted in lower than expected crude protein intake.

• Reducing crude protein intake to 8.3% reduced feed intake and tended to reduce ADG when summarized across the entire feeding period.

• No differences were detected in manure nutrient concentrations but the N:P ratio was increased when the lower CP diet was fed.

• No differences were detected in USDA Carcass Characteristics.

Summary