the next generation of dna assembly and cloning · * while supplies last. offer valid in the us...
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The Next Generation of
DNA Assembly and Cloning
NEW ENGLAND BIOLABS®, NEB® and NEBUILDER® are registered trademarks of New England Biolabs, Inc.
GIBSON ASSEMBLY® is a registered trademark of Synthetic Genomics, Inc.
* While supplies last. Offer valid in the US only. Limit one sample per customer.
NEBuilder® HiFi
DNA AssemblyThe next generation of DNA assembly andcloning has arrived. With NEBuilder HiFi DNAAssembly, you’ll enjoy virtually error-free joiningof DNA fragments. More efficient assemblyis now possible, even with larger fragments,low inputs, or 5´- and 3´-end mismatches.Additionally, use NEBuilder HiFi to bridge twodsDNA fragments with a ssDNA oligo. Save timewith less screening or re-sequencing, and benefitfrom no licensing fee requirements from NEBwhen choosing NEBuilder products.
Request a free sample*
at www.NEBuilderHiFi.com
Total fragmentsassembled
0
20
40
Fragment size
Vector size
Total size (kb)
60
80
100
NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621)
Gibson Assembly Master Mix (NEB #E2611)
PercentCorrect
2
A
3 kb
5.4 kb
8.4
10/10 10/10
2
B
210 bp
7.7 kb
7.9
3/35/5
6
F
5 x 1 kb frags.
3.3 kb
8.3
11/12
1/10
3
E
450 bp + 250 bp
7 kb
7.7
12/12
5/8
2
D
3 kb
2.1 kb
5.5
10/10
7/10
2
C
210 bp
7.7 kb
7.9
5/5
1/4
NEBuilder HiFi DNA Master Mix offers improved fidelity
over Gibson Assembly Master Mix
Fidelity of assembled products was compared between NEBuilder HiFI DNA Assembly Master Mix
(NEB #E2621) and Gibson Assembly Master Mix (NEB #E2611). Experiments were performed
using the various fragment and vector sizes, following suggested protocols. Experiments B and C vary
because sequences of fragments are different. Experiments D and F were performed with fragments
containing 3´-end mismatches.
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