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Page 1: 1Sources of DNA and Sequencing Methods.1Sources of DNA and Sequencing Methods 2 Genome Assembly Strategy and Characterization 2 Genome Assembly
Page 2: 1Sources of DNA and Sequencing Methods.1Sources of DNA and Sequencing Methods 2 Genome Assembly Strategy and Characterization 2 Genome Assembly
Page 3: 1Sources of DNA and Sequencing Methods.1Sources of DNA and Sequencing Methods 2 Genome Assembly Strategy and Characterization 2 Genome Assembly
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Page 5: 1Sources of DNA and Sequencing Methods.1Sources of DNA and Sequencing Methods 2 Genome Assembly Strategy and Characterization 2 Genome Assembly
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•..1Sources of DNA and Sequencing 1Sources of DNA and Sequencing MethodsMethods

• 2 Genome Assembly Strategy and 2 Genome Assembly Strategy and CharacterizationCharacterization

• 3 Gene Prediction and Annotation3 Gene Prediction and Annotation

• 4 Genome Structure4 Genome Structure

• 5 Genome Evolution5 Genome Evolution

• 6 A Genome-Wide Examination of 6 A Genome-Wide Examination of Sequence VariationsSequence Variations

• 7 An Overview of the Predicted Protein- 7 An Overview of the Predicted Protein- Coding Genes in the Human GenomeCoding Genes in the Human Genome

• 8 Conclusions8 Conclusions

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2.91 billion bp (Cellera) 3.2 billion (Hcon)

14.8 billions were sequenced in 9 months5.1 times overlap 8 times in genes

26,588 (sure) genes for proteins + 460 genes for RNAs+ ~12,000 sequences related to mouse etc.

1.1 % exons 24% introns 75% intergenic

About 50 % repeat sequences 45% transposables elements

Average length of a gene 27,894 basesExon ~100 bp , most: 234 in titin mRNAIntron ~100-30,000 bp

2.1 million SNPs, less than 1% of them in proteinsDifferences between human genomes 1 per 1250 bp

The Human Genome (Feb. 2001)

Page 21: 1Sources of DNA and Sequencing Methods.1Sources of DNA and Sequencing Methods 2 Genome Assembly Strategy and Characterization 2 Genome Assembly
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Unknown 36%

hydrolase

isomerase

Kinase 4%

Nucleic acid enzyme 13%

Page 27: 1Sources of DNA and Sequencing Methods.1Sources of DNA and Sequencing Methods 2 Genome Assembly Strategy and Characterization 2 Genome Assembly
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Genome Sequencing: Harmonia axyridis

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Next Generation Sequencing - Applications - MSImaths-people.anu.edu.au/~foret/next_gen_seq/hts_applications.pdf · 1 Genome sequencing De novo genome sequencing Genome resequencing

Class 02: Whole genome sequencing. The seminal papers ``Is Whole Genome Sequencing Feasible?'' ``Whole-Genome DNA

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articles Initial sequencing and analysis of the human genome...Initial sequencing and analysis of the human genome International Human Genome Sequencing Consortium* *A partial list

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Title of SMI goes here · Web viewYersinia pestis 97. Whole Genome Sequencing (WGS) This is also known as “full genome sequencing, complete genome sequencing, or entire genome sequencing”

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Genome Annotation - NDSUmcclean/plsc411/Genome... · Genome Annotation . Genome Sequencing • Costliest aspect of sequencing the genome o But Devoid of content • Genome must be

Sequencing a genome (a) outline the steps involved in sequencing the genome of an organism; (b) outline how gene sequencing allows for genome- wide comparisons

Whole genome sequencing

Genome Sequencing - NDSUmcclean/plsc411/Genome Sequencing...Genome Sequencing . ... Here the Phred scores are overlaid on the chromatogram of a Sanger sequencing output. ... o Directed

Sequencing the Maize (B73) Genome Genome Sequencing Center Maize Genome Sequencing Consortium

Sequencing the Human Genome€¦ · • Weber, J. L., & Myers, E. W. (1997). Human whole-genome shotgun sequencing. Genome Res, 7(5), 401-409. – Use clone end sequencing generating

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Validation of cytology media for whole genome sequencing · Validation of cytology media for whole genome sequencing: ... Validation of cytology media for whole genome sequencing:

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