Slide 1

The Microscope

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Definition A compound microscope is an optical instrument used to magnify and resolve fine detail within a transparent specimen. It has two separate lenses: (1) The objective lens and (2) the eyepiece

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Function of Microscope Parts

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Eyepiece The topmost optical lens system, also called the ocular. This is where the eye is positioned to view the object. Magnifies 10X Eyepiece

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Pointer Used to locate an object being viewed under the scope. Turning the eyepiece changes the pointer. Pointer (inside)

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Body Tube This is where the rays of light are bent so that the object being viewed is enlarged by the scope Body Tube

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Arm Supports the body tube and the objectives. Used to carry the microscope Arm

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Low Power Objective Magnifies 10x and is found above the specimen. Always begin focusing under low power Low Power (smallest objective)

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Medium Power Magnifies 25x. Use second after low power. Medium Power (middle sized objective)

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High Power Objective Magnifies 65x and is used last for greatest detail High Power (largest objective)

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Coarse Adjustment Knob The larger set of knobs used for coarse focusing under low power Coarse Adjustment Knob

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Fine Adjustment Knob Smaller set of focusing knobs used to control fine focusing under medium and high power Fine Adjustment Knob

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Base Supports the scope. Also used to carry the scope along with the arm Base

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Stage Flat area that supports the microscope slide Stage

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Stage Clips Hold the slide in place Stage Clips

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In-Stage Condenser Focuses and concentrates the light coming through the stage so that the object is correctly illuminated Aka aperture In-Stage Condenser

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Diaphragm The rotatable disc located beneath the stage that controls the amount of light entering the in- stage condenser Diaphragm

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Nosepiece Supports the revolving nosepiece Nosepiece

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Revolving Nosepiece Supports the objectives and is used to change from low to high Revolving Nosepiece

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Resolution Vs. Magnification

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Resolution The ability of a microscope to distinguish two objects as being separate

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Magnification The ability of a microscope to make objects appear larger.

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Resolution is more important than magnification!

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Magnification

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The magnification of the low power objective is 10x

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The magnification of the medium power objective is 25x

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The magnification of the high power objective is 65x

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The magnification of the eyepiece is 10x Eyepiece

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To find the Total Magnification you must Multiply Low Power X Eyepiece = Total magnification Under low power 10 x 10 = 100

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Carrying a Microscope

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1. One hand is placed around the arm and the other is placed under the base.

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2. Always hold in an upright position

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3. The stage clips should be turned inward

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4. Eyepeice at home position on low power.

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5. Body tube is in home position (all the way up)

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6. All slides have been put away and are no longer on the stage.

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7. Cord is unplugged and wrapped around the base

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Procedures for Focusing the Microscope

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1. Put the microscope on low power and turn the stage all the way down

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2. Place the microscope slide on the stage and secure with stage clips

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3. While looking through the eyepiece, turn the coarse adjuster toward you until the object comes into focus

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4. Turn the objective to medium power and refocus while looking through the eyepiece using the fine adjuster

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5. Turn the objective to high power and refocus while looking through the eyepiece using the fine adjuster http://www.youtube.com/watch?v=zDw_R3Y76yM&feature=related

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eyepiece pointer Arm Stage Clip Coarse Adjustment Fine Adjustment Power SwitchBase Light Diaphragm Stage (opening) In-Stage Condenser Objectives Revolving Nosepiece Nosepiece (whole thing) Body tube Stage Stop

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Lab Safety: What NOT to do! http://www.youtube.com/watch?v=vRIA04U796E&feature=related