The Mechanism of Protein Splicing:

**** the movie ****

Animation by Maurice W. Southworth

As seen in InBase, Francine Perler, Curator

CH2

SH

Cys

Intein

Asn

C-extein

NH

Cys

C

O

N-extein

NH C

O

CH2

SH

CH2

SH

N-extein Cys

Intein C-extein

Asn

C

O

NH

Cys

C

O

NH

CH2

SH

CH2

SH

N-exteinCys

Intein C-extein

Asn

C

O

NH

Cys

C

O

NH

CH2

SH

CH2

SH

N-extein

Cys

Intein C-extein

Asn

C

O

NH

Cys

C

O

NH

CH2

SH

CH2S

N-extein

Cys

Intein C-extein

Asn

C

O

NH

Cys

C

O

NH2

CH2

SH

Intein C-extein

Asn

C

O

NH

Cys

CH2

SH

CH2

SN-extein

Cys

C

O

NH2

Intein

C-extein

Asn

C

O

NH

CH2

SH

CH2

SN-extein

Cys

C

O

NH2

Intein

C-extein

Asn

C

O

NH

CH2

SH

CH2

SN-extein

Cys

C

O

NH2

Intein

C-extein

Asn

C

O

NH

CH2

SH

CH2

SN-extein

Cys

NH2

C

O

CH2

N-extein

Cys

Intein

C-extein

Asn

C

O

CH2

SH

S

C

O

NH

NH2

CH2

N-extein

Cys

Intein

C-extein

Asn

C

O

CH2

SH

S

C

O

NH

NH2

CH2

N-extein

Cys

Intein

C-extein

Asn

C

O

CH2

SH

S

C

NH

O

NH2

CH2

N-extein

Cys

Intein

C-extein

Asn

C

O

NH

CH2S

SH

C

O

NH2

CH2

N-extein

Cys

Intein

C-extein

Asn

C

O

CH2S

SH

C

O

NH2

NH

N-extein

Cys

Intein

C-extein

Asn

C

O

CH2

SC

O

NH2

CH2

SH

NH

N-extein

Cys

Intein

C-extein

Asn

C

O

NH

CH2

SC

O

NH2

CH2

SH

N-extein

Cys

Intein

C-extein

Asn

C

O

NH

CH2

S

C

O

NH2

CH2

SH

N-extein

Cys

Intein

C-extein

Asn

C

O

NH

CH2

S

C

O

NH2

CH2

SH

N-extein

Cys

Intein

C-extein

Asn

C

O

NH

CH2

S

C

O

NH2

CH2

SH

N-extein

Cys

Intein

C-extein

Asn

C

O

NH

CH2

S

C

O

NH2

CH2

SH

N-extein

Cys

Intein

C-extein

Asn

C

O

NH

CH2

S

C

O

NH2

CH2

C

NH2

CH2

SHO

N-extein

Cys

Intein

C-extein

Asn

C

O

NH

CH2

S

C

O

NH2

CH2

C

NH2

CH2

SHO

N-extein

Cys

Intein

C-extein

Asn

C

O

NH

CH2

S

C

O

NH2

CH2

C

NH2

CH2

SHO

N-extein

Cys

Intein

C-extein

Asn

C

O

NH

CH2

S

C

O

NH2

CH2

C

NH2

CH2

SHO

N-extein

Cys

Intein

C-extein

Asn

C

O

NH

CH2

S

C

O

NH2

CH2

C

NH2

CH2

SHO

N-extein

Cys

Intein

C-extein

Asn

C

O

NH

CH2

S

C

O

NH2

CH2

CNH2

CH2

SHO

N-extein

Cys

Intein

C-extein

Asn

C

O

NH

CH2

S

C

O

NH2

CH2

NH2

CH2

SHC

O

N-extein

Cys

Intein

C-extein

Asn

C

O

NH

CH2

S

C

O

NH2

CH2

NH2

CH2

SHC

O

N-extein

Cys

Intein

C-extein

Asn

C

O

NH

CH2

S

C

O

NH2

CH2

NH2

CH2

SHC

O

N-extein

Cys

Intein

C-extein

Asn

C

O

NH

CH2

S

C

O

NH2

CH2NH2

CH2

SHC

O

N-extein

Cys

Intein

C-extein

Asn

C

O

NH

CH2

S

C

O

NH2

CH2NH2

CH2

SHC

O

N-extein

Cys

Intein

C-extein

Asn

C

O

NH

CH2

S

C

O

NH2

CH2NH2

CH2

SHC

O

N-extein

Cys

Intein

C-extein

Asn

C

O

NH

CH2

S

C

O

NH2

CH2NH2

CH2

SHC

O

N-extein

Cys

Intein

C-extein

Asn

C

O

NH

CH2

S

C

O

NH2

CH2NH2

CH2

SHC

O

N-extein

Cys

Intein

C-extein

Asn

C

O

NH

CH2

S

C

O

NH2

CH2NH2

CH2

SHC

O

N-extein

Cys

Intein

C-extein

Asn

C

O

NH

CH2

S

C

O

NH2

CH2NH2

CH2

SHC

O

N-extein

Cys

Intein

C-extein

Asn

C

O

NH

CH2

S

C

O

NH2

CH2NH2

CH2

SHC

O

N-extein

Cys

Intein

C-extein

Asn

C

O

NH

CH2

S

C

O

NH2

CH2NH2

CH2

SHC

O

N-extein

Cys

Intein

C-extein

Asn

C

O

NH

CH2

S

C

O

NH2

CH2NH2

CH2

SHC

O

N-extein

Cys

Intein

C-extein

Asn

C

O

NH

CH2

S

C

O

NH2

CH2NH2

CH2

SHC

O

N-extein

Cys

Intein

C-extein

Asn

C

O

NH

CH2

S

C

O

NH2

CH2NH2

CH2

SHC

O

N-extein

Cys

Intein

C-extein

Asn

C

O

NH

CH2

S

C

O

NH2

CH2NH2

CH2

SHC

O

N-extein

Cys

Intein

C-extein

NH2

CH2

S

C

O

NH2

CH2

SH

Asn

C

O

NHCH2

C

O

N-extein

Cys

Intein

C-extein

NH2

CH2

S

C

O

NH2

CH2

SH

Asn

C

O

NHCH2

C

O

N-extein

Cys

Intein

C-extein

NH2

CH2

S

C

O

NH2

CH2

SH

Asn

C

O

NHCH2

C

O

N-extein

Cys

Intein

C-extein

NH2

CH2

S

C

O

NH2

CH2

SH

Asn

C

O

NHCH2

C

O

N-extein

Cys

Intein

C-extein

NH2

CH2

S

C

O

NH2

CH2

SH

Asn

C

O

NHCH2

C

O

N-extein

Cys

Intein

C-extein

NH2

CH2

S

C

O

NH2

CH2

SH

Asn

C

O

NHCH2

C

O

N-extein

Cys

Intein

C-extein

NH2

CH2

S

C

O

NH2

CH2

SH

Asn

C

O

NHCH2

C

O

N-extein

Cys

Intein

C-extein

NH2

CH2

S

C

O

NH2

CH2

SH

Asn

C

O

NHCH2

C

O

N-extein

Cys

Intein

C-extein

NH2

CH2

S

C

O

NH2

CH2

SH

Asn

C

O

NHCH2

C

O

N-extein

Cys

Intein

C-extein

NH2

CH2

S

C

O

NH2

CH2

SH

Asn

C

O

NHCH2

C

O

N-extein

Cys

Intein

C-extein

NH2

CH2

S

C

O

NH2

CH2

SH

Asn

C

O

NHCH2

C

O

N-extein

Cys

Intein

C-extein

NH2

CH2

S

C

O

NH2

CH2

SH

Asn

C

O

NHCH2

C

O

N-extein

Cys

Intein

C-extein

NH2

CH2

S

C

O

NH2

CH2

SH

Asn

C

O

NHCH2

C

O

N-extein

Cys

Intein

C-extein

NH2

CH2

S

C

O

NH2

CH2

SH

Asn

C

O

NHCH2

C

O

N-extein

Cys

Intein

C-extein

NH2

CH2

S

C

O

NH2

CH2

SH

Asn

C

O

NHCH2

C

O

N-extein

Cys

Intein

C-extein

NH2

CH2

S

C

O

NH2

CH2

SH

Asn

C

O

NHCH2

C

O

N-extein

Cys

Intein

C-extein

NH2

CH2

S

C

O

NH2

CH2

SH

Asn

C

O

NHCH2

C

O

N-extein

Cys

Intein

C-extein

NH2

CH2

S

C

O

NH2

CH2

SH

Asn

C

O

NHCH2

C

O

N-extein

Cys

Intein

C-extein

NH2

CH2

S

C

O

NH2

CH2

SH

Asn

C

O

NHCH2

C

O

N-extein

Cys

Intein

C-extein

NH2

CH2

S

C

O

NH2

CH2

SH

Asn

C

O

NHCH2

C

O

N-extein

Cys

Intein

C-extein

NH2

CH2

S

C

O

NH2

CH2

SH

Asn

C

O

NHCH2

C

O

N-extein

Cys

Intein

C-extein

NH2

CH2

S

C

O

NH2

CH2

SH

Asn

C

O

NHCH2

C

O

N-extein

Cys

Intein

C-extein

NH2

CH2

S

C

O

NH2

CH2

SH

Asn

C

O

NHCH2

C

O

N-extein

Cys

Intein

C-extein

NH2

CH2

S

C

O

NH2

CH2

SH

Asn

C

O

NHCH2

C

O

N-extein

Cys

Intein

C-extein

NH2

CH2

S

C

O

NH2

CH2

SH

Asn

C

O

NHCH2

C

O

N-extein

Cys

Intein

C-extein

NH2

CH2

S

C

O

NH2

CH2

SH

Asn

C

O

NHCH2

C

O

N-extein

Cys

Intein

C-extein

NH2

CH2

S

C

O

NH2

CH2

SH

Asn

C

O

NHCH2

C

O

N-extein

Cys

Intein

C-extein

NH2

CH2

S

C

O

NH2

CH2

SH

Asn

C

O

NHCH2

C

O

Cys

Intein

C-extein

NH2

CH2

NH2

CH2

SH

Cys

Asn

C

O

NHCH2

C

O

N-exteinS

C

O

Cys

Intein

C-extein

NH2

CH2

NH2

CH2

SH

Cys

Asn

C

O

NHCH2

C

O

N-exteinS

C

O

Cys

Intein

C-extein

NH2

CH2

NH2

CH2

SH

Cys

Asn

C

O

NHCH2

C

O

N-exteinS

C

O

Cys

Intein

C-extein

NH2

CH2

NH2

CH2

SH

Cys

Asn

C

O

NHCH2

C

O

N-exteinS

C

O

Cys

Intein

C-extein

NH2

CH2

NH2

CH2

SH

Cys

Asn

C

O

NHCH2

C

O

N-exteinS

C

O

Cys

Intein

C-extein

NH2

CH2

NH2

CH2

SH

Cys

Asn

C

O

NHCH2

C

O

N-exteinS

C

O

Cys

Intein

C-extein

NH2

CH2

NH2

CH2

SH

Cys

Asn

C

O

NHCH2

C

O

N-exteinS

C

O

Cys

Intein

C-extein

NH2

CH2

NH2

CH2

SH

Cys

Asn

C

O

NHCH2

C

O

N-exteinS

C

O

Cys

Intein

C-extein

NH2

CH2

NH2

CH2

SH

Cys

Asn

C

O

NHCH2

C

O

N-exteinS

C

O

Cys

Intein

C-extein

NH2

CH2

NH2

CH2

SH

Cys

Asn

C

O

NHCH2

C

O

N-exteinS

C

O

Cys

Intein

C-extein

NH2

CH2

NH2

CH2

SH

Cys

Asn

C

O

NHCH2

C

O

N-exteinS

C

O

Cys

Intein

C-extein

NH2

CH2

NH2

CH2

SH

Cys

Asn

C

O

NHCH2

C

O

N-exteinS

C

O

Cys

Intein

C-extein

NH2

CH2

NH2

CH2

SH

Cys

Asn

C

O

NHCH2

C

O

N-exteinS

C

O

N-extein

Cys

Intein

C-extein

NH2CH2

S

C

O

NH2

CH2

SHCys

Asn

C

O

NHCH2

C

O

N-extein

Cys

Intein

C-extein

NH2CH2

S

C

O

NH2

CH2

SHCys

Asn

C

O

NHCH2

C

O

N-extein

Cys

Intein

C-extein

NH2

CH2

SC

O

NH2

CH2

SHCys

Asn

C

O

NHCH2

C

O

N-extein

Cys

Intein

C-extein

NH2

CH2

SC

O

NH2

CH2

SHCys

Asn

C

O

NHCH2

C

O

N-extein

Cys

Intein

C-extein

NHCH2

SH

C

O

NH2

CH2

SHCys

Asn

C

O

NHCH2

C

O

N-extein

Cys

Intein

C-extein

NHCH2

SH

C

O

NH2

CH2

SHCys

Asn

C

O

NHCH2

C

O

N-extein

Cys

Intein

C-extein

NH

CH2

SH

C

O

NH2

CH2

SH

Cys

Asn

C

O

NHCH2

C

O

N-extein

Cys

Intein

C-extein

NH

CH2

SH

C

O

NH2

CH2

SH

Cys

Asn

C

O

NHCH2

C

O

N-extein

Cys

Intein

C-extein

NH

CH2

SH

C

O

NH2

CH2

SH

Cys

Asn

C

O

NHCH2

C

O

N-extein C-extein

NH

CH2

SH

C

O

Cys

Cys

Intein

NH2

CH2

SH

Asn

C

O

NHCH2

C

O

N-extein C-extein

NH

CH2

SH

C

O

Cys

Cys

Intein

NH2

CH2

SH

Asn

C

O

NHCH2

C

O

N-extein C-extein

NH

SH

C

O

Cys

Cys

Intein

NH2

CH2

SH

Asn

C

O

NHCH2

C

O

CH2

N-extein C-extein

NH

CH2

SH

C

O

Cys

Cys

Intein

NH2

CH2

SH

Asn

C

O

NHCH2

C

O

N-extein C-extein

NH

CH2

SH

C

O

Cys

Cys

Intein

NH2

CH2

SH

Asn

C

O

NHCH2

C

O

N-extein C-extein

NH

CH2

SH

C

O

Cys

Cys

Intein

NH2

CH2

SH

Asn

C

O

NHCH2

C

O

N-extein C-extein

NH

CH2

SH

C

O

Cys

Cys

Intein

NH2

CH2

SH

Asn

C

O

NHCH2

C

O

N-extein C-extein

NH

CH2

SH

C

O

Cys

Cys

Intein

NH2

CH2

SH

Asn

C

O

NHCH2

C

O