the mechanism of enzymatic action
DESCRIPTION
The Mechanism of Enzymatic Action. Figure 5.4a. Enzyme Inhibitors: Competitive Inhibition. Figure 5.7a–b. Enzyme Inhibitors: Competitive Inhibition Example-Sulfa drugs (sulfonamides) Discovered in the 1930s. Oxidation-Reduction. Figure 5.9. Representative Biological Oxidation. Figure 5.10. - PowerPoint PPT PresentationTRANSCRIPT
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Enzyme Inhibitors: Competitive Inhibition Example-Sulfa drugs (sulfonamides) Discovered in the 1930s
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The Generation of ATP ATP is generated by the phosphorylation of ADP
1. Substrate-level Phosphorylation2. Oxidative Phosphorylation3. Photophosphorylation
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Substrate-Level Phosphorylation
A chemical reaction where a phosphate group is transferred from one molecule to ADP. This requires a specific enzyme that can transfer the phosphate from this specific molecule to ADP.
ATP is produced this way during FERMENTATION Glycolysis (or alternative pathways) Krebs cycle
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Oxidative Phosphorylation
Energy released from transfer of electrons (oxidation) from one compound to another (reduction) is used to generate ATP in the electron transport chain
An electron transport chain(ETC) couples a chemical reaction between an electron donor (such as NADH) and an electron acceptor (such as O2) to the transfer of H+ ions across a membrane, through a set of mediating biochemical reactions. http://en.wikipedia.org/wiki/Electron_transport_chain
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Photophosphorylation
Light causes chlorophyll to give up electrons. The electrons go through a process similar to what happens during respiration (an electron transport chain and chemiosmosis occur). This process releases energy used to bond a phosphate to ADP producing ATP.
The ATP produced is used to produce food molecules (sugars-glucose).
Copyright © 2010 Pearson Education, Inc.Figure 5.11
Glycolysis
The oxidation of glucose to pyruvic acid produces ATP (Substrate level phosphorylation)and NADH
2 Stages: See next 2 slides
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Energy Using Stage of Glycolysis
2 ATP are used Glucose is split to form 2 glucose-3-phosphate
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ATP Creating Stage of Glycolysis
2 glucose-3-phosphate oxidized
to 2 pyruvic acid 4 ATP produced
Substrate-level phosphorylation 2 NADH produced
Copyright © 2010 Pearson Education, Inc.Figure 5.13
Preparatory Step Intermediate between Glycolysis and Krebs Cycle Pyruvic acid (from glycolysis) is oxidized and
decarboyxlated
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Pathway Eukaryote Prokaryote
Glycolysis Cytoplasm Cytoplasm
Intermediate step Cytoplasm Cytoplasm
Krebs cycle Mitochondrial matrix Cytoplasm
ETC Mitochondrial inner membrane Plasma membrane
Comparing Eukaryotic and Prokaryotic Cellular Location of Catabolic Processes
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Aerobic and Anaerobic Respiration
Aerobic respiration: The final electron acceptor in the electron transport chain is molecular oxygen (O2).
Anaerobic respiration: The final electron acceptor in the electron transport chain is not O2. Yields less energy than aerobic respiration because only part of the Krebs cycles operates under anaerobic conditions.
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Electron Acceptor Products
NO3– NO2
–, N2 + H2O
SO4– H2S + H2O
CO32 – CH4 + H2O
Anaerobic Respiration
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Fermentation
FERMENTATION Scientific definition: Releases energy from oxidation of organic molecules Does not use oxygen Does not use the Krebs cycle or ETC Uses an organic molecule (pyruvic acid) as the final
electron acceptor to form ‘end-products’ (acids and alcohols)
2 ATPs netted
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Photosynthesis
Conversion of light energy into chemical energy (ATP) which is used to synthsize nutrients (glucose)
Overall Summary Reaction? Compare and Contrast: Oxidative Phosphorylation
and Photophosphorylation.
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Photosynthesis
Oxygenic:
Anoxygenic:
2 2
6 12 6 2 2
6 CO + 12 H O + Light energy
C H O + 6 H O + 6 O
2 2
6 12 6 2
6 CO + 12 H S + Light energy
C H O + 6 H O + 12 S
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Nutritional Type Energy Source Carbon Source Example
Photoautotroph Light CO2 Oxygenic: Cyanobacteria plantsAnoxygenic: Green, purple bacteria
Photoheterotroph Light Organic compounds
Green, purple nonsulfur bacteria
Chemoautotroph Chemical CO2 Iron-oxidizing bacteria
Chemoheterotroph Chemical Organic compounds
Fermentative bacteriaAnimals, protozoa, fungi, bacteria.
Metabolic Diversity among Organisms