“the instructions for assembling every organism on the planet--slugs and sequoias, peacocks and
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“The instructions for assembling every organism on the planet--slugs and sequoias, peacocks and parasites, whales and wasps--are all specified in DNA sequences that can be translated into digital information and stored in a computer for analysis. As - PowerPoint PPT PresentationTRANSCRIPT
“The instructions for assembling every organismon the planet--slugs and sequoias, peacocks andparasites, whales and wasps--are all specified in DNAsequences that can be translated into digital information and stored in a computer for analysis. As a consequence of this revolution, biology in the 21st century is rapidly becoming an information science...
...hypotheses will arise as often in silico as in vitro.”
Eric Lander, Science 287 (5459), 1777-1782
The Problem …analysis of native state assembly of proteins.
• Protein function and folding are highly cooperative processes,
• Amino acids that interact in these processes can be close, or relatively distant in the 1o structure,
– identifying interacting residues in active sites, or identifying interacting residues that yield discrete 3o structure is difficult,
– these interactions are not obvious by scanning primary sequence.
A Partial Solution
• Mutational analysis,
– clone the gene,
– alter the DNA sequence that codes for specific residues,
– express the gene,
– check for function or conformational fidelity.
Labor intensive. Doesn’t indicate residue interactions.
A Better SolutionThermodynamic Mutant Cycling Analysis
More later…but briefly…
Double mutation analysis,
– used to determine if two different residues (or peptide fragments) interact.
Labor intensive.
Presently impossible to accomplish on a large scale.
A Bioinformatic Alternative…...let Evolution do the dirty work.
Multiple Sequence Alignment (globins)
“Entropy” in a MSA…the key to this paper.
• Think of amino acids as parts of a system that follows the rules of thermodynamics,
– if there were no constraints, amino acid frequency and distribution would tend to randomness,
– however, natural selection constrains primary sequence in living systems.
MSA and Entropy
Genomic SequencesDNA Sequence:Reagent for the 21st Century
PDZ Domains (n = 274)
“Model” Protein Domain Family
• Evolutionarily conserved, especially in tertiary structure,
– C atoms: root mean square deviation = 1.4 angstroms*,
• More diverged in sequence homology,
– averaging 24% AA sequence similarity.
• *Four high resolution crystal structures of distantly related family members.
Post synaptic density protein (PSD95), Drosophila disc large tumor suppressor (DlgA), and Zonula occludens-1 protein (zo-1)
Structural Classification of Proteinsdomains
Google: SCOP
Pfam
PDZ domains are found in diverse signaling proteins in bacteria, yeasts, plants, insects and vertebrates. They bind either the carboxyl-terminal sequences of proteins or internal peptide sequences
PDZ domains consist of 80 to 90 amino acids comprising six beta-strands (betaA to betaF) and two alpha-helices, A and B, compactly arranged in a globular structure. Peptide binding of the ligand takes place in an elongated surface groove as an antiparallel beta-strand interacts with the betaB strand and the B helix. The structure of PDZ domains allows binding to a free carboxylate group at the end of a peptide through a carboxylate-binding loop between the betaA and betaB strands.
Don’t Sweat the Formulas!
…English Translation: a measure of conservation can be made by comparing the frequency of amino acids in the column of a MSA, to a randomly filled column…
…expressed as a change in free energy.
Figure 1A
Black: amino acid frequency in a database of 36,498 proteins.
Gray: amino acid frequency in a database of 274 PDZ domains.
PDZ domain AA 76
• AA 76 is known to be important in determining ligand specificity,
- S/T - X- V/I - COO- - or - - F/Y - X- V/A - COO-
Antepenultimate AA in the ligand.
Figure 1B,CPDZ MSA Gstat
Highly conserved.
Poorly conserved.
Gstat = 3.83 kT*
Gstat = 0.1 kT*
Figure 1D
76
99
Figure 1E, F
Coupled Sites?
…English Translation: you change the MSA by removing a subset of peptides that have similar (or identical) amino acids in a specific column…
…if the amino acid in the original column interacts with another part of the peptide, you might expect to see a change in Gstat (Gstat ) in another column of the new MSA.
Perturbing the MSA…extract subsets of low-entropy alignments.
Re-calculate Gstat in the new MSA, look
for columns that had a change in
Gstat.
AA 76
– removed all of the peptides that had a histidine at AA 76 in the MSA,
• Calculated the change in Gstat (Gstat) at all positions.
Figure 2 B AA 76
AA 34
AA 63
Figure 2C-F
33, 34, 80, 84local
29, 26other side of ligand binding
66, 57, 51unexpected
in Silica, So Far, So What?
Show me the money...
Fig. 3
Statistical Gstat
Experimental Gstat
H76Y
FRET Förster Resonance Energy Transfer
Mutant Cycling Analysis (General)…with FRET (Förster Resonance Energy Transfer)
ratio m1
ratio m1:m2
If not equal, then sites are coupled.
Please Note: this was a general presentation, see slide 31 for the manner used in this paper.
Fig. 3
?
Fig. D: What is it, why is it included?
Figure 4
Attempt to map connectivity through the peptide.
Also performed analysis on POZ domain.
Conclusion
With growing sequence data from evolutionary distant genomes, the mapping
of energetic connectivity for many fold families should be a realistic goal.
Figure 4
k (wt:wt) x k (mut:mut)
k (wt:mut) x k (mut:wt)
Coupling Coefficient(Mutant Cycling Analysis)
coupling coefficient =
...if there is no coupling, then the coupling coefficient would approach unity.