THE IMPORTANCE OF LABELLING YOUR S(TUFF) In the Globe and Mail this weekend there was a little anecdote that went as

follows:PhD student spends three years in tropical rainforest laboriously collecting lizard

excrement for some sort of analysis.The PhD student stores the excrement in the lab. freezer in an unlabelled plastic bag.The PhD student returns to rainforest to collect more excrement (apparently this stuff

is difficult to get!)The PhD student returns home.

Finds out all his excrement has been thrown out in a lab clean-up.PhD student says his career and life are ruined.

Let this tragedy be a warning to us all.

THE RER/GOLGI SYSTEM

The Nobel Prize in Physiology or Medicine

1974."for their discoveries

concerning the structural and functional organization

of the cell"

George Palade

Christian de Duve INTRACELLULAR PATHWAY OF SECRETORY PROTEIN TRAFFIC

LYSOSOMES/PEROXISOMES

Charles Leblond,

McGill University

Marian Neutra,

Harvard Medical School

ROLE OF THE GOLGI BODY

Palade is credited with being the first one to describe the small particles that later came to be known as ribosomes.

HOW DID PALADE AND CO-WORKERS ELUCIDATE THE PATHWAY OF SECRETORY PROTEIN TRAFFIN IN CELLS?

HOW DID NEUTRA AND LEBLOND ELUCIDATE THE ROLE OF THE GOLGI BODIES IN PPROTEIN SECRETION?

CHOOSE THE BEST CELLS TO WORK WITH!

PALADE AND CO-WORKERS DIGESTIVE CELLS OF PANCREAS

NEUTRA AND LEBLOND SMALL INTESTINE

FIBROBLAST

OSTEOCYTE

(2) USE THE BEST TECHNIQUES

PALADE AND CO-WORKERS DIGESTIVE CELLS OF PANCREAS

NEUTRA AND LEBLOND SMALL INTESTINE

(1) CHOOSE THE BEST CELLS TO WORK WITH!

MICROAUTORADIOGRAPHY

DUCT

ZYMOGENGRANULES

MITOCHONDRIA

ACINARCELLS

ROUGH ENDOPLASMICRETICULUM

BASEMENT LAMINA

DUODENUM

β-PARTICLES

3H HELIUM + β-PARTICLE

USE THE AMINO ACID LEUCINE IN WHICH AN R-GROUP 1H-ATOM HAS BEEN REPLACED WITH AN 3H-ATOM

SECRETORY VESICLES

SECRETORY VESICLES WITH PROTEIN MADE DURING THE EXPERIMENT

DECAY TRACK OF ONE β-PARTICLE

www.ncbi.nlm.nih.gov/bookshelf/picrender.fcgi...

PULSE-CHASE EXPERIMENT

Charles Leblond,

McGill University

Marian Neutra,

Harvard Medical School

ROLE OF THE GOLGI BODY

WANTED TO STUDY ROLE OF GOLGI BODY

USED 3H-FUCOSE (A SUGAR) INSTEAD OF 3H- LEUCINE (AN AMINO ACID)

GOBLET CELLS FROM EPITHELIUM OF SMALL INTESTINE

GOBLET CELLS OF RAT INTESTINE

THE MECHANISM OF POLYPETIDE IMPORT INTO THE RER

PAGE 697Post-translational import

MELANOSOMESCENTRAL VACUOLE

INTEGRAL MEMBRANE PROTEINSP 687

Co-translational import

GUNTHER BLOBEL

SIGNAL HYPOTHESIS

HOW ARE THE INTEGRAL MEMBRANE PROTEINS INSERTED

INTO THE RER MEMBRANE?

p703

p702 THE “STOP TRANSFER SEQUENCE” CAN SERVE AS THE SIGNAL SEQUENCE

HOW THE POLYPEPTIDE CAN MOVE LATERALLY OUT OF THE

TRANSLOCON

TRANSLOCON

THE ROLE OF CHAPERONES IN POLYPEPTIDE IMPORT

INTO THE RER

HEAT SHOCK PROTEINS IN FRUIT FLIES

Hsp = HEAT SHOCK PROTEIN

CHROMOSOMAL PUFFS

POLYTENE CHROMOSOMEENDOMITOSIS

MULTIPLE SISTER CHROMATIDS

Hsp90

CHAPERONES (NOT BARRELS)

CHAPERONINS (BARRELS)

NH3+

(AAAAA)n

mG

Hsp70PORE PROTEIN OF TRANSLOCON

NH3+

COO-

FOLDED PROTEIN

ATP

ADP +Pi

Elizabeth Craig

UNIVERSITY OF WISCONSIN