TEAM MEMBERSTEAM MEMBERS

Dan Carlson Team LeaderDan Carlson Team Leader

Dave Ugai CommunicatorDave Ugai Communicator

Erik Bieging BWIG/BSACErik Bieging BWIG/BSAC

““THE SOLUTIONATORS”THE SOLUTIONATORS”

From left: Dave Ugai, Erik Bieging, and Dan Carlson

CLIENTSCLIENTSJinling Wang, Ph.D.Jinling Wang, Ph.D.

Research AssociateResearch Associate

Department of PhysiologyDepartment of Physiology

Gail A. Robertson, Ph.D.Gail A. Robertson, Ph.D.Associate ProfessorAssociate Professor

Department of PhysiologyDepartment of Physiology

ADVISORADVISORProfessor John G. Webster, Ph.D.

ABSTRACTABSTRACTThe objective of the project is to design an The objective of the project is to design an automated system of valves for a solution automated system of valves for a solution delivery system to be used in patch clamp delivery system to be used in patch clamp research. The current system has two to eight research. The current system has two to eight solution reservoirs suspended in the air above solution reservoirs suspended in the air above a manifold. The manifold allows the a manifold. The manifold allows the researcher to manually control which solution researcher to manually control which solution is being delivered. This is a tedious process is being delivered. This is a tedious process for the researcher, so an automated system is for the researcher, so an automated system is desired.desired.

PROBLEM STATEMENTPROBLEM STATEMENT

The goal of this project was to The goal of this project was to develop a computer-automated develop a computer-automated system for delivering solutions to a system for delivering solutions to a patch clamp for physiological patch clamp for physiological laboratory research.laboratory research.

PATCH CLAMPPATCH CLAMP Method used to study Method used to study

single cellssingle cells HERG potassium HERG potassium

channel is critical in channel is critical in repolarization of the repolarization of the heart’s action potentialheart’s action potential

Long QT syndrome is a Long QT syndrome is a disease that can lead to disease that can lead to cardiac arrhythmia or cardiac arrhythmia or sudden deathsudden death MICRO PIPET OVER ION CHANNELMICRO PIPET OVER ION CHANNEL

http://www.iac-usnc.org/Methods/wholecell/equipment.htmlhttp://www.iac-usnc.org/Methods/wholecell/equipment.html

CURRENT SETUPCURRENT SETUP

Solutions are fed to a manifold and as the orientation of the multi-position stop-cocks is changed, the solution being delivered changes

MOTIVATIONMOTIVATION

•The current method for switching solutions is manually changing the alignment of valves on a manifold

•This is a tedious process that wastes valuable research time

CLIENT REQUIREMENTSCLIENT REQUIREMENTS

Must reliably open and close valves on Must reliably open and close valves on command from the computer command from the computer

Compatible with up to eight feeds of Compatible with up to eight feeds of medical tubingmedical tubing

Must generate minimal electrical and Must generate minimal electrical and mechanical noise (so as not to disturb mechanical noise (so as not to disturb delicate physiological research)delicate physiological research)

VARIABLE DESIGN VARIABLE DESIGN COMPONENTSCOMPONENTS

1.1. Valves: solenoid or solenoid pinch Valves: solenoid or solenoid pinch valve (both normally closed)valve (both normally closed)

2.2. Valve Controller: Valvelink8, Valve Controller: Valvelink8, Controlled 8 relay driver, Manually Controlled 8 relay driver, Manually assembled controller assembled controller

SOLENOID VALVESSOLENOID VALVES

When current runs through the solenoid the When current runs through the solenoid the plunger is drawn up and the valve opensplunger is drawn up and the valve opens

SOLENOID PINCH VALVESSOLENOID PINCH VALVES

The spring loaded plunger pinches the tubing, and The spring loaded plunger pinches the tubing, and when current runs through the solenoid, the plunger when current runs through the solenoid, the plunger is pulled back.is pulled back.

DECISION MATRIXDECISION MATRIX

Valvelink8Valvelink8 R85PROR85PRO Manually Manually assembledassembled

PricePrice 11 55 99

SimplicitySimplicity 1010 88 44

ReliabilityReliability 1010 99 77

TotalTotal 2121 2424 2020

FINAL DESIGN COMPONENTSFINAL DESIGN COMPONENTS

1.1. R85PRO relay driver (1)R85PRO relay driver (1)

2.2. 1/8 inch solenoid valves, normally closed (8)1/8 inch solenoid valves, normally closed (8)

3.3. RSIO Adapter (1)RSIO Adapter (1)

4.4. Serial cable (1)Serial cable (1)

5. 8-into-1 Teflon micro manifold with flow control (1)

6. NPT Male Pipe adapter (1/8” x 1/16”) (16)

MOUNTED DESIGNMOUNTED DESIGN1. R85PRO Driver

2. Solenoid valves

3. RSIO adapter1

2

3

The design was mounted on foam pads in a plastic box to reduce the mechanical noise produced by the valves

FUTURE WORKFUTURE WORK

Install device in client’s laboratoryInstall device in client’s laboratory

Configure with pClamp data acquisitionConfigure with pClamp data acquisition softwaresoftware

Provide instruction to client on usageProvide instruction to client on usage

REFERENCESREFERENCES Jack Commins, sales representative, AutoMate Jack Commins, sales representative, AutoMate

ScientificScientific L. Burke O’Neal, Biomedical Engineering Instrument L. Burke O’Neal, Biomedical Engineering Instrument

Innovator, UW Madison Innovator, UW Madison Ryan Sheldon, owner, National Control Devices, Ryan Sheldon, owner, National Control Devices,

controlanything.comcontrolanything.com James A. Sullivan, author, www.cellsalive.comJames A. Sullivan, author, www.cellsalive.com American Heart Association, website, American Heart Association, website,

www.americanheart.orgwww.americanheart.org