Teaching innovations in upper-division instructional chemistry laboratories at Regis University

Surendra N. Mahapatro, Department of Chemistry, Regis

University, Denver, Colorado 80221.

Some of the Challenges

Massive data collection with little insight

Disconnect between upper division laboratories including physical, analytical, inorganic and biochemistry

Relevance

• Synthesis and Characterization Laboratory Spring 2014

• Synthesis and Characterization of Vandyl bis-acetylacetonoate Complex

VANDYL BIS-ACETYLACETONOATE COMPLEX

Synthesis reported in 1957insulin mimetic properties

http://en.wikipedia.org/wiki/File:Vanadyl-acetylacetonate-from-xtal-3D-balls.png

Synthesis

IR: V= O STRETCH AT 992.1 CM-1

UV-VIS SPECTRA

CRYSTAL FIELD DIAGRAM

H1NMR-DETERMINATION OF MAGNETIC SUSCEPTIBILITY

EPR SPECTRUM OF THE VANADYL-COMPLEX

Magnetic Susceptibility Balance

4 Fe2+-cytochrome c + 8 H+in + O2

4 Fe3+-cytochrome c + 2 H2O + 4 H+

out

ISOLATION, PURIFICATION AND CHARACTERIZATION OF BEEF-HEART CYTOCHROME C OXIDASE

Aerobic life

RETRIEVED FROM: HTTP://WWW.BIOSCIENCE.ORG/2004/V9/AF/1237/FULLTEXT.

ASP?BFRAME=FIGURES.HTM&DOI=YES

Yoshikawa et al Science, 269, 1069-1074, 1995; ibid, Science,1996, 272, 1136-1144

Neurodegenerative diseases: Alzheimer’s and Parkinson’sLeigh syndrome (LS).

n-Dodecyl β-D-maltoside (Lauryl maltoside)

Cytochrome c Oxidase 

Heme analysis (nine micromoles of heme per gram of protein)  Molecular activity (kinetics- spectrophotometry and oxygen electrode assay) Cyanide (suicide substrate) binding Subunit integrity- SDS-PAGE

Lenny Carruthers, PhD (UTHSCSA) Christina Closken, MD (Creighton)Baltazor Gomez , PhD (UTHSCSA) Natalie Gibson, PhD (UTHSCSA)Joaquin Urbina, PhD (KSU) Kenroy Crawford, PhD (Purdue)Anthony Giordion, PhD (Georgia Tech) Angello Aguilar; PhD (KSU)Aplolonio Aguilar, PhD (UT Austin) Heather Brungart, PhD (CU Boulder)Lisa Brassell, PhD (CU Boulder) Carissa Cawich, MDHong T. Lee, DO (Turo, CA) Nhan Pham, DO (Turo College, CA)Ryan Farmer, MD (CU, Medical center) David Pinsinski, MD (Creighton)Clifford Everett, MD (George Town) Charles Tate , MD (Univ. Colorado)Karen Link, MS (UTHSC, San Antonio) Andrew Wallocot, PhD (Pen Sate)Elizabeth Forbes, MD (Univ. Colorado) Katie Srock, MD (Univ. Colorado)

 

REGIS ALUMNI (CHEMISTRY MAJORS) RESEARCH ASSOCIATES

 

Jacob Garcia Micah FernandezAndreina LujanSara RodriguezJennifer Campos

CURRENT UNDERGRADUATE RESEARCH ASSOCIATES

• Research Corporation-Cottrell College science grant• ACS-PRF (type B) grant• NIH-AREA• NSF-ILI• Department of Energy-Rocky Flats• NREL –subcontract• Gates Foundation of Colorado-Partners in Science grant• Pittsburgh Memorial Analytical Conference: College Science

grant program• Western Alliance to Expand student opportunities

(WAESO); Hispanic Research Center, Arizona State University

 

GRANT SUPPORT

• During the past 7 years, 32 students were supported by undergraduate research stipends.

WAESO

• Prof. Michael Doyle, University of Maryland, College Park, Maryland

• Fr. William T. Miller, S.J., Regis University, Denver, Colorado

• Prof. Fraser Armstrong, Oxford • Prof. Duy Hua, KSU• Prof. Brindavan Ranu, Indian Association for the

Cultivation of Science, Calcutta• Fullbright Foundation

Acknowledgement: