talinum paniculatum
TRANSCRIPT
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TALINUM PANICULATUM (GINSENG JAWA)
20 DAYS
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GROUP MEMBERS… NATASHA KAUR AMARPREET KAUR YAASHINI
SELVARAJAH KRISNAVIMALA
KRISHUAN NABILAH BINTI
YUSOF NORIZA BINTI
ISHAK
AMIRAH BINTI SHAARI
HILDA ANAK EMAK AIN NAJWA BINTI
AMRAN NURUL HIDAYAH
BINTI MANSOR ONG SHWU CHYN PUI WAN TING WONG SIEW CHING
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INTRODUCTION
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Five Mus musculus have been
used to investigate the effect of Talinum Paniculatum (ginseng jawa) to the liver, kidney and intestine within `20 days of experimental.
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Problem statement
Why do we choose Mus musculus as our specimen?
• Class of mammalian• Easy to get• Easy to handle-small in size
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Purpose of the study
To know the effect of Talinum Paniculatum size and body weight of Mus musculus
Behaviour investigation of the Mus musculus.
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Significance of study Talinum Paniculatum contains
vitamin c and toxic
Tanin, Saponin and Flavoid.
So we carry out this experiment to see the side effects of Tanilum Paniculatum to the internal organs (intestine, kidney and liver) of Mus musculus.
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Research limitation
Experiment applicable only to five Mus musculus.
only three leaves of Tanilum Paniculatum used everyday.
Time limitation to only 20 days
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LITERATURE REVIEW
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1.Talinum:
A Summer Vegetable For Florida
DAVID FAIRCHILD, COCONUT GROOVE, FLORIDA
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DR J.L HEID said that in his report :In the green leaves of Talinum
The Vitamin C is about 160 mg per 100 gram
The Talinum show the phenomenally high content of vitamin C compared to guavas and sweet peppers.
It also probably has a considerable contents of carotenoid pigments.
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2The Effect Of Ginseng Jawa Root (Talinum Paniculatum) To The White Male Mencit
Evi Setiya ManuputyFaculty Of Pharmacy
University Of Muhammadiyah Surakarta
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Chemical content in Talinum paniculatum composed of saponin, flavanoid and tanin.
Saponin - strengthen our body and - improve blood
circulation. Saponin : can cause irritation in
mucosa membrane, and can damage red blood cell through hemolysis
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The root can be used to treat : tiredness, dizzy, diarrhea, aphrodisiacs and pneumonia.
The leaves can be used to treat bump. Also increase the sexual desire in male
mencit.
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Materials and methods
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20 days of observation5 mice (Mus musculus)Bedding3 ginseng jawa (Talinum paniculatum)
leaves (0.5g) mix with 100 ml waterMice food
Mice is weighed daily Amount of water consumed are
measured
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DissectionGloveCotton and chloroformDissecting setDissecting tray and pinsWaterBeaker
Water was applied on the ventral side of mice
The kidney, small intestine and liver were dissected.
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Stages/technique in preparing slide
Fixation. Dehydration. Clearing. Embedding. Sectioning. Coloring/ staining.
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FixationBouin’s fluid (<24 hours)Bottles
DehydrationEthanol 70% (< 24 hours)Ethanol 80%, 95%, 100% (< 1 hour)
ClearingToluene solution (< 24 hours)
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Embedding (Paraffin processing)
Paraffin solution (60C) (< 1 hour) Paraffin solution (60C) (< 1 hour)
• (twice procedure)
Paraffin solution added in mold followed by the organ.
Tissue sample then was left for 5 days.
The infiltrating medium (paraffin) provides cellular support for cutting tissue sections.
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Sectioning (Microtome /cutting) materials methods
Diamond penDistilled waterAlbuminSlide and slide
coverMicrotomeBrush
The slices was placed on the slide.
Few drops of distilled water/albumin are dropped on the slides. Intestine-distilled
waterAlbumin - liver and
kidney Heating (30C)
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Coloring/staining
Materials Methods Xylen 1 And 2 Alcohol+xylen (1:1) Alcohol,
Alcohol( 90%,80%,75%) Distilled Water Hematoksilin Solution 0.5 % Sodium Bicarbonate
Solution Running Water Scott’s Solution Eosin
Color stain used :• Harris Hematoksilin- Eosin
coloring.
• Soaking of slides- • into 21 piece of jar
containing different types of chemicals and liquid
• based on the duration given.
• Gluing: DPX / Canada balsam
• Heating of slides • (37 C) • 1 week
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Results & discussion
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day 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17Resu
lt0
5
10
15
20
25
30
12345
Graph showing the increasing weight mass of the mice in 20 days
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The weight of each mice increase day by day After taking Talinum paniculatum as their drink.
The Talinum paniculatum rich with vitamin Cincreases appetiteGaining of body weight
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Graph showing the amount of water and Talinum paniculatum consumed by the mice's
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Intestine (duodenum)
Affected intestine
The goblet cell in this intestine secretes more mucous compared to the normal intestine
40x magnification
Goblet cell
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LIVER
Affected liver Normal slide
Wide spaces in between cells showing the sinusoid. This means that more enzymes are secreted to detoxify the drug (ginseng jawa). In the normal slide the sinusoid is compact.
sinusoid
40 x magnification
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The presence of vitamin C in Talinum paniculatum causes more enzyme to be released to detoxify the drugs in the liver cells.
prove: white spaces (sinusoid)seen in effected liver cells while the original cell the (sinusoid) is more compact.
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KIDNEY
Affected kidney Normal kidney
The glomerular is tightly packed due to the toxic caused by the drug that is being detoxify by the glomerular as compared to the normal kidney slide.
glomerulus
40x magnification
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Materials & function:• act as rinsing agent to remove the blood from
the organs, and its sterileNormal saline solution
• a fixing & preserving solution for the organsBouin’s solution-
• For dehydration processEthanol 70%,80%,95% & 100%
• used for the clearance purposeToluene
• to hold the organ tissues tightly during the sectioning process
paraffin
• Only the small intestine cell can withstand with the pressure.Distilled water
• to maintain the shape of the kidney and liver cell.Albumin
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Effect of Talinum
paniculatum
Increase the appetite
Improve blood circulation
Stimulate testosterone hormone to
enhance sexual desire
they will get attracted to mate becomes active and aggressive
Weight gain
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Tannins precipitate proteins inhibits in some ruminant animals for the absorption of nutrients from high-tannin
grains such as sorghum.
Tannic acid does not affect absorption
of other trace minerals such as zinc, copper, and manganese in rats
Prove: the glomerulus at kidney cell is tightly packed compared to the original slide of kidney where there are some spaces between the glomerulus.
The lethal dose of Talinum paniculatum around 6% is able to increase the animal's body weight.
Prove: the mice’s body weight increases drastically in that 20 days.
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Problem about the project
Damage of the weighing machine.
Damage of Microscope.
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The end..Thank you..