table 1. suppl. primers used for real-time qpcr analysis.10.1007... ·  · 2017-05-19primers used...

12
Table 1. Suppl. Primers used for real-time qPCR analysis. Gene name Unigene ID Sequence (5ʹ-3ʹ) C4H 54449 CTGGTAAATCTCACGGGTCATGGGAAGAGTCCATTACTTTGGTCAG CHS 10609 TGGGTGTCTTGTTTGGATTTGGCGGATTACTACACACACTTACAAGCC F3H 1667 CCACTATTTTCTCTCTCACTTTCCA CCACCGAACTGCCTTTACCT FLS 44780 GCCAACGGAAGTGAAACAGAGATGAGGAGGAAGATAGTGAAGGAA 625 ACCCTGTCCAAGACCAGACCCCACCGAACTGCCTTTACCT OMT 49313 ACGCCAAATGATAACTACGCTTCTCGCAGTTCTCTCTTTACCACCT 49335 CCTTATGGTTTGGTGTTGTGGCTCGCAAATGGTAATCCCGTCTC F3'H 22319 CAACAAGCTCAAGATTCTTTCATGCATCGTCAGTGTCGTCGTCC F3'5'H 42301 CACGAGATTGATGCTGGACGATCATTATTCTTGCTCCTACCGC MYBF1 11090 ACAACAAGAAGAAGCACGATGGGGGAAAGGATGACTGGTAGGTG Actin GGAATCCACGAGACCACCTACAGATTGATCCTCCGATCCAGACA Table 2 Suppl. UPLC-MS data of compounds (CE - collision energy, RT - retention time). Compounds Precursors Fragmentors CE Product ion RT [min] Calibration curves R 2 Dihydromyricetin 319.1 105 12 193 2.38 y=2098.66*x-7.81 0.999 Taxifolin 303.1 115 12 285 3.47 y=2177.73*x+29.39 0.999 Isorhamnetin-3- O-neohespeidoside 623.1 160 40 314 4.62 y=566.66*x+14.16 0.9999 Quercetin 301.1 130 30 115 8.23 y=1195.89*x+38.01 0.9999 Isorhamnetin 315.1 120 20 300 9.04 y=14231.54*x+1487.50 0.9906 Kaempferide 299.1 130 22 284 9.55 y=6115.72*x+84.67 0.9998 Hyperoside 463.1 170 27 300 4.75 y=5547.72*x+1.09 0.9999 Myricetin 317.1 130 28 151 5.79 y=975.88*x+83.00 0.9989 Rutin 609.1 200 35 300 4.93 y=2416.98*x+26.20 0.9997

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Table 1. Suppl. Primers used for real-time qPCR analysis.

Gene name Unigene ID Sequence (5ʹ-3ʹ)

C4H 54449 CTGGTAAATCTCACGGGTCATGGGAAGAGTCCATTACTTTGGTCAG

CHS 10609 TGGGTGTCTTGTTTGGATTTGGCGGATTACTACACACACTTACAAGCC

F3H 1667 CCACTATTTTCTCTCTCACTTTCCA CCACCGAACTGCCTTTACCT

FLS 44780 GCCAACGGAAGTGAAACAGAGATGAGGAGGAAGATAGTGAAGGAA

625 ACCCTGTCCAAGACCAGACCCCACCGAACTGCCTTTACCT

OMT 49313 ACGCCAAATGATAACTACGCTTCTCGCAGTTCTCTCTTTACCACCT

49335 CCTTATGGTTTGGTGTTGTGGCTCGCAAATGGTAATCCCGTCTC

F3'H 22319 CAACAAGCTCAAGATTCTTTCATGCATCGTCAGTGTCGTCGTCC

F3'5'H 42301 CACGAGATTGATGCTGGACGATCATTATTCTTGCTCCTACCGC

MYBF1 11090 ACAACAAGAAGAAGCACGATGGGGGAAAGGATGACTGGTAGGTG

Actin GGAATCCACGAGACCACCTACAGATTGATCCTCCGATCCAGACA

Table 2 Suppl. UPLC-MS data of compounds (CE - collision energy, RT - retention time).

Compounds Precursors Fragmentors CE Product ion RT [min] Calibration curves R2

Dihydromyricetin 319.1 105 12 193 2.38 y=2098.66*x-7.81 0.999

Taxifolin 303.1 115 12 285 3.47 y=2177.73*x+29.39 0.999

Isorhamnetin-3-

O-neohespeidoside

623.1 160 40 314 4.62 y=566.66*x+14.16 0.9999

Quercetin 301.1 130 30 115 8.23 y=1195.89*x+38.01 0.9999

Isorhamnetin 315.1 120 20 300 9.04 y=14231.54*x+1487.50 0.9906

Kaempferide 299.1 130 22 284 9.55 y=6115.72*x+84.67 0.9998

Hyperoside 463.1 170 27 300 4.75 y=5547.72*x+1.09 0.9999

Myricetin 317.1 130 28 151 5.79 y=975.88*x+83.00 0.9989

Rutin 609.1 200 35 300 4.93 y=2416.98*x+26.20 0.9997

Table 3 Suppl. Number of unigenes related to the flavonoids synthesis pathway under NaCl-treatment in

Reaumuria trigyna (Deg/all - the ratio of DEGs among the annotated unigenes).

Gene All Deg Upregulated Downregulated Deg/all [%] Up/deg [%]

PAL 11 4 0 4 36 0

C4H 9 1 1 0 11 100

4CL 23 5 4 1 22 80

CHS 11 6 2 4 55 33

CHI 6 1 0 1 17 0

F3H 8 3 3 0 38 100

FLS 6 6 4 2 100 57

F3’H 2 2 1 1 100 50

F3’5’H 3 2 1 1 67 50

UFGT 5 5 2 3 100 40

OMT 6 6 6 0 100 100

DFR 4 4 0 4 100 0

IFR 5 5 0 5 100 0

LDOX 3 2 1 1 60 50

CYP450 16 16 16 0 100 100

Table 4 Suppl. Putative transcription factors related to the flavonoid synthesis pathway in Reaumuria trigyna (Fold

change - relative fold-increase in expression of target genes in NaCl stress as compared to respective control.)

Gene name Gene ID Gene

length [bp]

Accession Fold

change

Identity

[%]

Function Reference

MYBF1 unigene11090 947 gi|289623206|

Vitis vinifera

8.22 79 regulation of flavonol synthesis (Czemmel et al.

2009)

MYB5b unigene4486 648 gi|526117769|

Vitis vinifera

1.13 78 regulation of anthocyanin and

proanthocyanidin biosynthesis

(Deluc et al.

2008)

MYB1 unigene14669 449 gi|23476279|

Gossypium hirsutum

0.63 81 regulation of polyphenols

accumulation

(Bomal et al.

2008)

unigene15466 1440 gi|23476279|

Gossypium hirsutum

3.73 72

MYB3 unigene972 678 gi|2921333|

Lotus japonicus

0.56 82 regulating anthocyanin

biosynthesis in flower

(Nakatsuka et al.

2008)

unigene 1212 1088 gi|731317153|

Arabidopsis thaliana

0.38 80

MYCA1 unigene13791 1902 gi|526118174|

Vitis vinifera

1.00 75 related to flavonoid synthesis (Matus et al.

2010)

bHLH3 unigene1295 992 gi|38490122|

Cornus capitata

1.27 78 regulation of anthocyanin

biosynthesis pathway

(Espley et al.

2007)

unigene29229 964 gi|302398604|

Malus x domestica

0.97 80

MYC1 unigene 19039 1214 gi|33339703|

Catharanthus roseus

0.55 75 promotion of anthocyanin

accumulation in grape cells

(Hichri et al.

2010)

unigene55994 581 gi|33339703|

Catharanthus roseus

1.32 78

MYB32 unigene 46082 252 gi|71041091|

Malus x domestica

5.62 78 regulation of polyphenols

accumulation

(Dubos et al.

2010)

WRKY23 unigene14033 1913 gi|42570003|

Arabidopsis thaliana

2.95 79 control of flavonol

biosynthesis

(Grunewald et

al. 2012)

Gen

e na

me

Gen

e ID

G

ene

Len

gth

C21

_raw

read

s (8

4865

31)

C21

_RPK

M

T43

_raw

read

s (9

5602

48)

T43

_RPK

M

log2

(T43

_RPK

M

/C21

_RPK

M)

Ann

otat

ion

phen

ylal

anin

e am

mon

ia-ly

ase,

PA

L

Uni

gene

10

418_

All

283

96

39.9

719

130

48.0

494

0.26

55

gi|1

8803

7480

|gb|

AB

N79

671.

2|

[Rud

beck

ia h

irta

] U

nige

ne

1179

8_A

ll 89

6 38

1 50

.105

7 15

2 17

.744

6 -1

.497

6 gi

|295

0026

04|g

b|A

DF5

9061

.1|

[Pyr

us x

bre

tsch

neid

eri]

Uni

gene

13

929_

All

1840

97

2 62

.247

11

74

66.7

392

0.10

05

gi|2

5350

9589

|gb|

AC

T320

33.1

| [G

lyci

ne so

ja]

Uni

gene

17

23_A

ll 21

41

522

28.7

292

157

7.67

03

-1.9

052

gi|3

0243

60|sp

|Q42

667.

1|

[Citr

us li

mon

] U

nige

ne

3038

8_A

ll 35

9 42

5 13

9.49

68

404

117.

7112

-0

.245

gi

|224

0687

33|re

f|XP_

0023

2618

6.1|

[P

opul

us tr

icho

carp

a]

Uni

gene

30

947_

All

267

62

27.3

622

58

22.7

221

-0.2

681

gi|2

4191

2463

|gb|

AC

S719

53.1

| [E

upho

rbia

pul

cher

rim

a]

Uni

gene

39

79_A

ll 31

1 50

18

.944

3 13

4.

3723

-2

.115

3 gi

|241

9124

63|g

b|A

CS7

1953

.1|

[Eup

horb

ia p

ulch

erri

ma]

U

nige

ne

6291

6_A

ll 29

3 40

4 16

2.47

39

333

118.

8796

-0

.450

7 gi

|190

7107

09|g

b|A

CE9

5170

.1|

[Pop

ulus

tom

ento

sa]

Uni

gene

79

35_A

ll 45

1 16

8 43

.893

7 71

16

.466

9 -1

.414

4 gi

|224

0687

33|re

f|XP_

0023

2618

6.1|

[P

opul

us tr

icho

carp

a]

Uni

gene

80

63_A

ll 59

6 66

4 13

1.27

79

553

97.0

532

-0.4

358

gi|2

3768

2387

|gb|

AC

R10

243.

1|

[Can

ariu

m a

lbum

] U

nige

ne

9595

_All

221

125

66.6

481

134

63.4

225

-0.0

716

gi|5

6409

806|

emb|

CAH

1768

6.1|

[B

eta

vulg

aris

]

Table5Supp

l.Cand

idateun

igen

esann

otated

toflavono

idbiosynthe

sispathw

ayand

differentia

llyexpressedbetweencontrol(C2

1)and

Na

Cl-treatedsamples(T

43)inRe

aumuriatrigyna.

Gen

e na

me

Gen

e ID

G

ene

Len

gth

C21

_raw

read

s (8

4865

31)

C21

_RPK

M

T43

_raw

read

s (9

5602

48)

T43

_RPK

M

log2

(T43

_RPK

M

/C21

_RPK

M)

Ann

otat

ion

cinn

amat

e-4-

hydr

oxyl

ate,

C4H

Uni

gene

14

792_

All

520

19

4.30

55

15

3.01

73

-0.5

129

gi|9

8283

650|

gb|A

BF5

8026

.1|

[Hib

iscu

s can

nabi

nus]

U

nige

ne

1936

5_A

ll 38

7 41

1 12

5.14

13

317

85.6

799

-0.5

465

gi|4

7933

894|

gb|A

AT3

9513

.1|

[Cam

ptot

heca

acu

min

ata]

U

nige

ne

2007

3_A

ll 31

2 63

8 24

0.95

5 44

0 14

7.51

25

-0.7

079

gi|2

8402

2951

|gb|

AD

B65

927.

1|

[Ipo

moe

a ba

tata

s]

Uni

gene

21

626_

All

620

904

171.

8092

58

8 99

.201

1 -0

.792

4 gi

|237

6823

85|g

b|A

CR

1024

2.1|

[C

anar

ium

alb

um]

Uni

gene

26

866_

All

737

34

5.43

6 46

6.

5286

0.

2642

gi

|237

6823

85|g

b|A

CR

1024

2.1|

[C

anar

ium

alb

um]

Uni

gene

26

989_

All

557

74

15.6

548

146

27.4

175

0.80

85

gi|2

8402

2951

|gb|

AD

B65

927.

1|

[Ipo

moe

a ba

tata

s]

Uni

gene

32

040_

All

370

153

48.7

259

255

72.0

89

0.56

51

gi|8

4578

865|

dbj|B

AE7

2875

.1|

[Ver

bena

x h

ybri

da]

Uni

gene

54

449_

All

663

16

2.84

37

42

6.62

62

1.22

04

gi|8

4578

865|

dbj|B

AE7

2875

.1|

[Ver

bena

x h

ybri

da]

Uni

gene

83

52_A

ll 67

5 42

9 74

.889

9 52

9 81

.975

2 0.

1304

gi

|161

5977

79|g

b|A

BX

7477

9.1|

[R

ubus

cor

eanu

s]

4-co

umar

ate-

coal

igas

e,

4CL

Uni

gene

11

762_

All

424

251

69.7

554

308

75.9

829

0.12

34

gi|2

2413

6077

|ref|X

P_00

2322

234.

1|

[Pop

ulus

tric

hoca

rpa]

U

nige

ne

1857

5_A

ll 82

1 17

2.

4399

43

5.

4784

1.

1669

gi

|224

0859

96|re

f|XP_

0023

0777

0.1|

[P

opul

us tr

icho

carp

a]

Uni

gene

18

690_

All

1882

34

0 21

.287

7 38

5 21

.397

9 0.

0074

gi

|224

1219

30|re

f|XP_

0023

1870

8.1|

[P

opul

us tr

icho

carp

a]

Uni

gene

18

960_

All

721

50

8.17

16

58

8.41

44

0.04

22

gi|2

2407

4401

|ref|X

P_00

2304

364.

1|

[Pop

ulus

tric

hoca

rpa]

U

nige

ne

1901

2_A

ll 80

3 12

5 18

.342

7 81

10

.551

2 -0

.797

8 gi

|224

1330

70|re

f|XP_

0023

2795

4.1|

[P

opul

us tr

icho

carp

a]

Gen

e na

me

Gen

e ID

G

ene

Len

gth

C21

_raw

read

s (8

4865

31)

C21

_RPK

M

T43

_raw

read

s (9

5602

48)

T43

_RPK

M

log2

(T43

_RPK

M

/C21

_RPK

M)

Ann

otat

ion

4-co

umar

ate-

coal

igas

e,

4CL

Uni

gene

21

064_

All

1503

47

0 36

.847

6 24

6 17

.120

1 -1

.105

9 gi

|294

5169

38|g

b|A

DE9

6997

.1|

[Sor

bus a

ucup

aria

] U

nige

ne

2298

1_A

ll 23

9 46

9 23

1.23

03

441

193.

0063

-0

.260

7 gi

|187

2367

98|g

b|A

CD

0213

5.1|

[P

anic

um v

irga

tum

] U

nige

ne

2310

1_A

ll 33

5 6

2.11

05

38

11.8

651

2.49

11

gi|2

2408

5996

|ref|X

P_00

2307

770.

1|

[Pop

ulus

tric

hoca

rpa]

U

nige

ne

2606

0_A

ll 76

1 93

7 14

5.08

57

1138

15

6.41

86

0.10

85

gi|2

2412

6051

|ref|X

P_00

2329

649.

1|

[Pop

ulus

tric

hoca

rpa]

U

nige

ne

2843

1_A

ll 69

4 17

9 30

.392

3 12

6 18

.990

7 -0

.678

4 gi

|294

5169

38|g

b|A

DE9

6997

.1|

[Sor

bus a

ucup

aria

] U

nige

ne

2874

3_A

ll 70

7 31

3 52

.166

9 37

2 55

.037

0.

0773

gi

|224

1219

30|re

f|XP_

0023

1870

8.1|

[P

opul

us tr

icho

carp

a]

Uni

gene

55

989_

All

2314

11

0 5.

6014

21

6 9.

7639

0.

8017

gi

|224

0775

16|re

f|XP_

0023

0528

2.1|

[P

opul

us tr

icho

carp

a]

Uni

gene

69

50_A

ll 19

90

877

51.9

298

2266

11

9.10

71

1.19

76

gi|2

2413

7016

|ref|X

P_00

2322

473.

1|

[Pop

ulus

tric

hoca

rpa]

U

nige

ne

8526

_All

483

16

3.90

34

12

2.59

88

-0.5

869

gi|2

6863

6247

|gb|

AC

Z173

92.1

| [V

itis v

inife

ra]

Uni

gene

91

29_A

ll 12

65

135

12.5

751

149

12.3

205

-0.0

295

gi|2

2413

3070

|ref|X

P_00

2327

954.

1|

[Pop

ulus

tric

hoca

rpa]

U

nige

ne

3011

1_A

ll 38

6 35

7 10

8.98

1 44

4 12

0.31

69

0.14

28

gi|9

6519

15|g

b|A

AF9

1309

.1|

[Rub

us id

aeus

] U

nige

ne

3444

8_A

ll 52

5 43

3 97

.184

8 50

7 10

1.01

35

0.05

57

gi|2

2412

1930

|ref|X

P_00

2318

708.

1|

[Pop

ulus

tric

hoca

rpa]

U

nige

ne

3484

4_A

ll 74

1 18

2.

8624

15

2.

1174

-0

.434

9 gi

|224

1219

30|re

f|XP_

0023

1870

8.1|

[P

opul

us tr

icho

carp

a]

Uni

gene

37

4_A

ll 92

5 90

3 11

5.03

12

1684

19

0.42

82

0.72

72

gi|2

2412

7758

|ref|X

P_00

2329

170.

1|

[Pop

ulus

tric

hoca

rpa]

Gen

e na

me

Gen

e ID

G

ene

Len

gth

C21

_raw

read

s (8

4865

31)

C21

_RPK

M

T43

_raw

read

s (9

5602

48)

T43

_RP

KM

lo

g2(T

43_R

PK

M/C

21_R

PKM

) A

nnot

atio

n

4-co

umar

ate-

coa

ligas

e,

4CL

Uni

gene

40

111_

All

316

84

3.37

73

35

1.43

49

8.37

07

gi|2

2407

4393

|ref|X

P_00

2304

363.

1|

[Pop

ulus

tric

hoca

rpa]

U

nige

ne

4138

1_A

ll 20

73

214

12.1

642

335

16.9

035

0.47

47

gi|2

2407

7516

|ref|X

P_00

2305

282.

1|

[Pop

ulus

tric

hoca

rpa]

U

nige

ne

4866

6_A

ll 37

2 66

3 21

0.01

02

1205

33

8.82

46

0.69

01

gi|2

2412

7758

|ref|X

P_00

2329

170.

1|

[Pop

ulus

tric

hoca

rpa]

U

nige

ne

5163

_All

1943

22

1 13

.402

6 40

4 21

.749

0.

6984

gi

|224

0859

96|re

f|XP_

0023

0777

0.1|

[P

opul

us tr

icho

carp

a]

chal

cone

sy

ntha

se,

CH

S

Uni

gene

10

609_

All

332

38

13.4

87

125

39.3

825

1.54

6 gi

|122

2961

3|sp

|Q9X

GX

2.1|

[S

orgh

um b

icol

or]

Uni

gene

10

759_

All

365

532

171.

7468

79

6 22

8.11

35

0.40

95

gi|2

9363

2014

|gb|

AD

E594

87.1

| [M

usa

acum

inat

a AA

A G

roup

] U

nige

ne

1782

8_A

ll 26

4 73

4 32

7.61

36

243

96.2

794

-1.7

667

sp|P

1795

7|

[Gly

cine

max

] U

nige

ne

2638

7_A

ll 37

4 12

88

405.

8019

53

0 14

8.22

97

-1.4

529

gi|3

2329

248|

gb|A

AP7

4755

.1|

[Gyp

soph

ila p

anic

ulat

a]

Uni

gene

26

797_

All

452

2292

59

7.51

11

866

200.

4058

-1

.576

gi

|300

8852

73|g

b|A

DK

4532

5.1|

[F

allo

pia

mul

tiflo

ra]

Uni

gene

33

350_

All

351

13

4.36

42

6 1.

788

-1.2

874

gi|1

5851

5815

|gb|

AB

W69

675.

1|

[Ipo

moe

a pu

rpur

ea]

Uni

gene

39

67_A

ll 30

1 21

4 83

.775

5 36

8 12

7.88

28

0.61

02

gi|3

4850

869|

dbj|B

AC

8786

3.1|

[T

oren

ia h

ybri

da]

Uni

gene

43

107_

All

208

284

160.

8884

37

1 18

6.56

98

0.21

37

gi|2

9336

939|

sp|Q

9FSC

0.1|

[R

uta

grav

eole

ns]

Uni

gene

49

878_

All

206

1 0.

572

2 1.

0155

0.

8281

gi

|867

5321

7|gb

|ABD

1473

8.1|

[R

uta

grav

eole

ns]

Uni

gene

54

191_

All

343

5 1.

7177

26

7.

9288

2.

2066

gi

|999

2378

1|gb

|AB

F688

80.1

| [C

arda

min

e pe

ctin

ata]

U

nige

ne

5960

1_A

ll 23

1 26

5 13

5.17

7 34

0 15

3.95

6 0.

1877

gi

|861

6157

2|gb

|AB

C86

919.

1|

[Pop

ulus

alb

a]

Gen

e na

me

Gen

e ID

G

ene

Len

gh

C21

_raw

read

s (8

4865

31)

C21

_RPK

M

T43

_raw

read

s (9

5602

48)

T43

_RPK

M

log2

(T43

_RPK

M

/C21

_RPK

M)

Ann

otat

ion

chal

cone

is

omer

ase,

C

HI

Uni

gene

23

656_

All

807

203

29.6

41

102

13.2

208

-1.1

648

gi|2

3994

9926

|gb|

AC

S366

62.1

| [V

itis l

abru

sca]

U

nige

ne

2501

7_A

ll 80

3 39

5.

7229

73

9.

5091

0.

7326

gi

|425

6934

2|re

f|NP_

1801

99.3

| [A

rabi

dops

is th

alia

na]

U

nige

ne

2379

9_A

ll 27

6 15

7 67

.028

6 17

2 65

.185

4 -0

.040

2 gi

|122

2334

81|sp

|Q45

QI7

.2|

[Cam

ellia

sine

nsis

] U

nige

ne

2956

5_A

ll 90

4 95

3 12

4.22

08

709

82.0

368

-0.5

986

gi|2

2411

9114

|ref|X

P_00

2331

328.

1|

[Pop

ulus

tric

hoca

rpa]

U

nige

ne

4002

_All

377

579

180.

9702

40

5 11

2.36

85

-0.6

875

gi|2

2470

8766

|gb|

AC

N60

401.

1|

[Cap

sicu

m a

nnuu

m]

Uni

gene

66

85_A

ll 39

9 15

8 46

.661

10

6 27

.788

4 -0

.747

7 gi

|170

7837

64|g

b|A

CB

3736

6.1|

[G

lyci

ne m

ax]

flava

none

3-

hydr

oxyl

ase

, F3H

Uni

gene

13

22_A

ll 10

23

926

106.

6609

68

9 70

.448

9 -0

.598

4 gi

|119

6570

97|g

b|A

BL8

6673

.1

|[Gos

sypi

um b

arba

dens

e]

Uni

gene

16

67_A

ll 12

63

175

16.3

269

128

10.6

008

-0.6

231

gi|2

9780

8467

|ref|X

P_00

2872

117.

1|

[Ara

bido

psis

lyra

ta su

bsp.

lyra

ta]

Uni

gene

29

546_

All

642

70

12.8

479

194

31.6

08

1.29

88

gi|1

1547

6924

|ref|N

P_00

1062

058.

1|

[Ory

za sa

tiva

Japo

nica

Gro

up]

Uni

gene

29

669_

All

1116

12

8 13

.515

17

7 16

.589

8 0.

2957

gi

|115

4769

24|re

f|NP_

0010

6205

8.1|

[O

ryza

sativ

a Ja

poni

ca G

roup

] U

nige

ne

3307

9_A

ll 43

5 79

21

.399

7 22

2 53

.382

1.

3188

gi

|115

4769

24|re

f|NP_

0010

6205

8.1|

[O

ryza

sativ

a Ja

poni

ca G

roup

] U

nige

ne

3421

_All

310

115

43.7

125

287

96.8

392

1.14

75

gi|2

2410

3505

|ref|X

P_00

2313

083.

1|

[Pop

ulus

tric

hoca

rpa]

U

nige

ne

4198

8_A

ll 68

9 59

10

.090

3 74

11

.234

2 0.

1549

gi

|240

2559

24|re

f|NP_

5674

91.5

| [A

rabi

dops

is th

alia

na]

Uni

gene

60

106_

All

237

207

102.

9181

12

5 55

.168

7 -0

.899

6 gi

|238

6252

81|g

b|A

CR

4797

6.1|

[J

ugla

ns re

gia]

Gen

e na

me

Gen

e ID

G

ene

Len

gth

C21

_raw

read

s (8

4865

31)

C21

_RP

KM

T

43_r

awre

ads

(956

0248

) T

43_R

PKM

lo

g2(T

43_R

PK

M/C

21_R

PKM

) A

nnot

atio

n

flavo

nol

synt

hase

, FL

S

Uni

gene

23

941_

All

237

381

189.

429

179

79.0

015

-1.2

617

gi|2

2104

7210

|gb|

AC

L980

52.1

| [C

amel

lia si

nens

is]

Uni

gene

44

780_

All

231

173

88.2

478

489

221.

4255

1.

3272

gi

|224

1035

03|re

f|XP_

0023

1308

2.1|

[P

opul

us tr

icho

carp

a]

Uni

gene

59

864_

All

234

4 2.

0143

36

16

.092

3 2.

998

gi|2

6888

28|g

b|A

AB

8887

8.1|

[P

runu

s arm

enia

ca]

Uni

gene

60

648_

All

244

622

300.

3795

33

2 14

2.32

43

-1.0

776

gi|2

2406

3126

|ref|X

P_00

2301

003.

1|

[Pop

ulus

tric

hoca

rpa]

U

nige

ne

625_

All

1404

15

6 13

.092

6 97

8 72

.862

3 2.

4764

gi

|184

0299

2|re

f|NP_

5666

85.1

| [A

rabi

dops

is th

alia

na]

Uni

gene

64

863_

All

526

6 1.

3441

25

2 50

.112

5 5.

2205

gi

|297

8065

77|re

f|XP_

0028

7117

2.1|

[A

rabi

dops

is ly

rata

subs

p. ly

rata

]

flavo

noid

3'

-hyd

roxy

lase

, F3

'H

Uni

gene

13

978_

All

1396

16

81

141.

8901

77

1 57

.769

7 -1

.296

4 gi

|144

2332

7|gb

|AA

K62

346.

1|

[Viti

s vin

ifera

] U

nige

ne

2231

9_A

ll 10

15

134

7.62

6 74

15

.556

4 1.

0285

gi

|304

2143

3|gb

|AA

P310

58.1

| [P

opul

us tr

icho

carp

a]

flavo

noid

3'

,5'-h

ydro

xyla

se,

F3

'5'H

Uni

gene

29

604_

All

785

210

31.5

224

247

32.9

123

0.06

22

gi|3

0421

433|

gb|A

AP3

1058

.1|

[Pop

ulus

tric

hoca

rpa]

U

nige

ne

4230

1_A

ll 20

1 2

0.52

04

1 1.

1725

1.

1719

gi

|224

1267

37|re

f|XP_

0023

1991

4.1|

[G

ossy

pium

hir

sutu

m]

Uni

gene

45

635_

All

245

10

4.80

95

1 0.

4269

-3

.493

9 gi

|297

8118

49|re

f|XP_

0028

7380

8.1|

[G

ossy

pium

hir

sutu

m]

UD

P fla

vono

id

gluc

osyl

tra

nsfe

rase

, U

FGT

Uni

gene

63

776_

All

340

7 2.

426

34

10.4

6 2.

1082

gi

|528

3967

6|db

j|BA

D52

003.

1|

[Mor

ella

rubr

a]

Uni

gene

20

183_

All

481

26

6.36

94

83

18.0

494

1.50

27

gi|2

0995

4711

|dbj

|BA

G80

546.

1|

[Viti

s vin

ifera

]

Gen

e na

me

Gen

e ID

G

ene

Len

gth

C21

_raw

read

s (8

4865

31)

C21

_RP

KM

T

43_r

awre

ads

(956

0248

) T

43_R

PK

M

log2

(T43

_RPK

M

/C21

_RPK

M)

Ann

otat

ion

UD

P fla

vono

id

gluc

osyl

tra

nsfe

rase

, U

FGT

Uni

gene

13

453_

All

408

150

43.3

212

28

7.17

84

-2.5

933

gi|2

2285

8290

|gb|

EEE9

5837

.1|

[P

opul

us tr

icho

carp

a]

Uni

gene

14

481_

All

939

259

32.5

015

78

8.68

88

-1.9

033

gi|2

9731

9645

|gb|

EFH

5006

7.1|

[A

rabi

dops

is ly

rata

subs

p. ly

rata

] U

nige

ne

9797

_All

276

241

102.

8911

12

0 45

.478

2 -1

.177

9 gi

|528

3967

6|db

j|BA

D52

003.

1|

[Dia

nthu

s car

yoph

yllu

s]

O-m

ethy

ltran

sfer

ase,

O

MT

Uni

gene

62

137_

All

270

1 0.

4364

44

17

.045

9 5.

2876

gi

|663

0734

|emb|

CAB

6421

7.1|

[A

rabi

dops

is th

alia

na]

Uni

gene

52

342_

All

330

1 0.

3571

25

7.

9242

4.

4719

gi

|224

0696

29|re

f|XP_

0023

2638

8.1|

[P

opul

us tr

icho

carp

a]

Uni

gene

49

313_

All

614

540

103.

6323

38

16

650.

086

2.64

92

gi|1

1546

5627

|ref|N

P_00

1056

413.

1|

[Pop

ulus

tric

hoca

rpa]

U

nige

ne

4933

5_A

ll 63

9 43

8 80

.768

7 27

40

448.

5187

2.

4733

gi

|663

0734

|emb|

CAB

6421

7.1|

[A

rabi

dops

is th

alia

na]

Uni

gene

28

478_

All

693

32

5.44

11

173

26.1

122

2.26

28

gi|4

7900

734|

gb|A

AT3

9306

.1|

[Pop

ulus

tric

hoca

rpa]

U

nige

ne

3367

4_A

ll 27

5 21

8.

9982

63

23

.962

9 1.

4131

gi

|228

4804

64|g

b|A

CQ

4189

3.1|

[P

runu

s mum

e]

isof

lavo

ne

redu

ctas

e,

IFR

Uni

gene

30

811_

All

323

831

303.

1575

45

9 14

8.64

18

-1.0

282

gi|1

4934

9541

|gb|

AB

R24

115.

1|

[Viti

s vin

ifera

] U

nige

ne

2330

5_A

ll 34

0 33

1 11

4.71

46

69

21.2

276

-2.4

34

gi|1

2306

14|g

b|A

AB

6772

9.1|

[Lup

inus

alb

us]

Uni

gene

67

93_A

ll 43

3 81

22

.042

8 33

7.

9718

-1

.467

3 p|

P525

81|

[Lup

inus

alb

us]

Uni

gene

10

289_

All

550

3712

79

5.27

09

1460

27

7.66

49

-1.5

181

gi|7

6559

894|

tpe|

CAI5

6334

.1|

[V

itis v

inife

ra]

Uni

gene

64

845_

All

525

1635

36

6.96

8 86

5 17

2.34

06

-1.0

904

sp|P

5257

7|

[Ara

bido

psis

thal

iana

]

Gen

e na

me

Gen

e ID

G

ene

Len

gth

C21

_raw

read

s (8

4865

31)

C21

_RPK

M

T43

_raw

read

s (9

5602

48)

T43

_RPK

M

log2

(T43

_RPK

M

/C21

_RPK

M)

Ann

otat

ion

Dih

ydro

flavo

nol

-4-r

educ

tase

, D

FR

Uni

gene

15

73_A

ll 98

9 11

5 13

.701

6 60

6.

3458

-1

.110

5 gi

|765

5989

4|tp

e|CA

I563

34.1

| [O

ryza

sativ

a Ja

poni

ca G

roup

] U

nige

ne

6323

_All

365

70

22.5

983

19

5.44

49

-2.0

532

gi|1

7084

24|sp

|P52

581.

1|

[Jat

roph

a cu

rcas

] U

nige

ne

2168

8_A

ll 70

6 12

93

215.

8061

55

6 82

.376

-1

.389

4 gi

|116

0779

90|d

bj|B

AF3

4846

.1|

[Sol

anum

dem

issu

m]

Uni

gene

43

60_A

ll 49

6 36

2 85

.999

7 17

4 36

.694

3 -1

.228

8 gi

|765

5989

4|tp

e|CA

I563

34.1

| [C

amel

lia o

leife

ra]

leuc

oant

hocy

ani

din

diox

ygen

ase,

LD

OX

Uni

gene

38

112_

All

509

28

6.48

2 22

4.

521

-0.5

198

gi|2

0260

150|

gb|A

AM

1297

3.1|

[A

rabi

dops

is th

alia

na]

Uni

gene

24

841_

All

549

14

3.00

49

470

89.5

481

4.89

73

gi|2

9780

6577

|ref|X

P_00

2871

172.

1|

[Ara

bido

psis

lyra

ta su

bsp.

lyra

ta]

Uni

gene

26

243_

All

473

28

6.97

54

12

2.65

37

-1.3

943

gi|1

7695

92|g

b|A

AB

3999

5.1|

[D

iant

hus c

aryo

phyl

lus]

Cyt

ochr

ome

P450

, C

YP4

50

Uni

gene

50

151_

All

427

5 1.

3798

56

13

.718

3.

3135

gi

|222

8468

37|g

b|EE

E843

84.1

| [P

opul

us tr

icho

carp

a]

Uni

gene

30

27_A

ll 83

3 18

2.

5462

15

3 19

.212

2 2.

9156

gi

|224

0716

32|re

f|XP_

0023

0354

6.1|

[P

opul

us tr

icho

carp

a]

Uni

gene

32

892_

All

339

17

5.90

91

119

36.7

179

2.63

55

gi|4

6359

653|

dbj|B

AD

1533

1.1|

[P

anax

gin

seng

] U

nige

ne

2752

7_A

ll 15

78

83

6.19

78

522

34.6

015

2.48

1 gi

|224

0716

32|re

f|XP_

0023

0354

6.1|

[P

opul

us tr

icho

carp

a]

Uni

gene

96

96_A

ll 61

5 29

5.

5564

15

5 26

.362

6 2.

2463

gi

|224

0586

39|re

f|XP_

0022

9957

9.1|

[P

opul

us tr

icho

carp

a]

Uni

gene

25

799_

All

1548

10

3 7.

8404

40

6 27

.433

8 1.

807

gi|4

6359

653|

dbj|B

AD

1533

1.1|

[P

anax

gin

seng

] U

nige

ne

1504

6_A

ll 16

83

71

4.97

1 27

0 16

.780

7 1.

7552

gi

|170

6591

6|em

b|CA

C80

883.

1|

[Cat

hara

nthu

s ros

eus]

Gen

e na

me

Gen

e ID

G

ene

Len

gth

C21

_raw

read

s (8

4865

31)

C21

_RP

KM

T

43_r

awre

ads

(956

0248

) T

43_R

PKM

lo

g2(T

43_R

PK

M/C

21_R

PKM

) A

nnot

atio

n

Cyt

ochr

ome

P450

, C

YP4

50

Uni

gene

25

113_

All

564

337

70.4

078

1269

23

5.34

95

1.74

1 gi

|224

1373

06|re

f|XP_

0023

2709

3.1|

[P

opul

us tr

icho

carp

a]

Uni

gene

32

915_

All

385

15

4.59

09

52

14.1

278

1.62

17

gi|2

2405

8639

|ref|X

P_00

2299

579.

1|

[Pop

ulus

tric

hoca

rpa]

U

nige

ne

2178

0_A

ll 78

3 21

3.

1603

69

9.

2176

1.

5443

gi

|850

0171

9|gb

|AB

C68

413.

1|

[Gly

cine

max

] U

nige

ne

1405

3_A

ll 18

21

288

18.6

36

834

47.9

057

1.36

21

gi|4

2569

342|

ref|N

P_18

0199

.3|

[P

opul

us tr

icho

carp

a]

Uni

gene

28

321_

All

1867

65

1 41

.087

2 17

46

97.8

207

1.25

15

gi|2

2406

3931

|ref|X

P_00

2301

307.

1|

[Pop

ulus

tric

hoca

rpa]

U

nige

ne

1582

4_A

ll 41

2 52

14

.872

2 12

2 30

.973

7 1.

0584

gi

|850

0170

9|gb

|AB

C68

408.

1|

[Ory

za sa

tiva

Japo

nica

Gro

up]

Uni

gene

30

263_

All

534

31

6.84

05

123

24.0

932

1.81

65

gi|2

2405

8639

|ref|X

P_00

2299

579.

1|

[Pop

ulus

tric

hoca

rpa]

U

nige

ne

5613

3_A

ll 39

3 2

0.59

97

22

5.85

55

3.28

75

gi|7

8183

422|

dbj|B

AE4

7005

.1|

[Cat

hara

nthu

s ros

eus]

U

nige

ne

2579

9_A

ll 15

48

103

7.84

04

406

27.4

338

1.80

7 gi

|224

1251

06|re

f|XP_

0023

2989

5.1|

[P

anax

gin

seng

]