syngas fermentation for bio-fuels production
TRANSCRIPT
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Syngas fermentation for bio-fuels production: Opportunities &
Summation
Presented By – Diksha Kumari
Mangalayatan university Aligarh
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introduction
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Time taking complex pre-treatment steps.
Low fermentibility of mixed sugar stream.
High enzyme cost.
Difficulty in utilization of whole
biomass.
Challenges in biochemical pathway
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Synthetic gas or syngas is a mixture mainly composed of carbon monoxide (CO), carbon dioxide (CO2), hydrogen (H2) and other by-products like tars, hydrocarbon, nitric oxide and ammonia.
syngas
Biochemical pathway Solution Syngas derived Biofuel
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Utilization of the whole biomass including lignin. Elimination of complex pre-treatment steps and costly
enzymes. Higher specificity of the biocatalysts. Independence of the H2:CO ratio for bioconversion. Aseptic operation of syngas fermentation due to
generation of syngas at higher temperatures Biological catalysts are able to ferment syngas into
liquid fuel effectively(Heiskanen et al., 2007; Henstra et al.,2007).
Why syngas...
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Gasification SyngasProcessing
Syngas Fermentation
SyncrudeRefining &Upgrading
X LG
Syngas processing
Ligno-cellulosicBiomass
Fuels&
Chemicals
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Incomplete utilization of lignocellulosic biomass
Low gas liquid mass transfer.
Low productivity.
Sensitivity of microorganisms to environmental conditions
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Gasification is required to overcome this hurdle.
Gasification of lignocellulosic biomass through partial
oxidation leads to the formation of gaseous mixture.
Biomass Gasification
2(-CH-) + O2 → 2CO + H2
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Incomplete utilization of lignocellulosic biomass
Low gas liquid mass transfer.
Low productivity.
Sensitivity of microorganisms to environmental
conditions
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Rate limiting step.
Gas liquid interface mass transfer is the major
resistance for gaseous substrate diffusion.
Leading to low substrate uptake by microbes.
Leading to low productivity.
GAS – LIQUID MASS TRANSFER
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Poor solubility of gaseous substrates in liquid phase is coped up by –
Optimizing the bioreactor design. (Bredwell et al., 1999). Increasing the agitation speed of the impeller (Bredwell et
al., 1999). Smaller bubbles sizes. Increasing the gas–liquid interfacial area for efficient mass
transfer.
Solution to GAS – LIQUID MASS TRANSFER
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Incomplete utilization of lignocellulosic biomass.
Poor solubility of gaseous substrates in liquid phase.
Low productivity.
Sensitivity of microorganisms to environmental conditions
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The identification of new microbial isolates that would
broaden the product range of syngas.
Thermophiles that employ CO as a substrate for the
production of chemicals could be selected based on the
identification of hydrogenase & CO dehydrogenase.
employ genetic engineering in carboxydotroph microbes
to enhance the product range of syngas fermentation
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Incomplete utilization of lignocellulosic biomass.
Poor solubility of gaseous substrates in liquid phase.
Low productivity.
Sensitivity of microorganisms to environmental conditions.
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Temperature….
By providing optimum temperature (eg- 37- 40°C for
mesophilles & 55-80°C for thermopiles').
Increases microbial growth and substrate utilization .
Increases solubility of gaseous substrate in aqueous
medium. (Younesi et al., 2005).
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Ph….. Important for optimal activity of microbial catalysts. Optimum pH ranges between 5.5 - 7.5 (depending
upon species) E.g.- C.ljungdahli - optimum Ph of 5.8 – 6.0. (Klasson
et al., 1993)
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Downstream
Midstream
Upstream
CONCLUSION
Optimization
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Upstream Processes - Strain development by metabolic engineering Use of inexpensive carbon substrate.
Midstream Process - Innovative fermentation strategies. Reduced environmental impact.
Downstream Process - In situ recovery & other cost effective recovery Geographically distributed supply source. Improve energy efficiency.
Conclusions
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A Joint study sponsored by U. S. Department of Energy (USDOE) and U.S. Department of agriculture (USDA). 2005. Biomass as feedstock for a bio energy and bioproducts industry: The technical feasibility of a Billion-ton annual supply, Oak Ridge, Tennessee.
Bredwell, M.D., Srivastava, P., Worden, R.M., 1999. Reactor design issues for synthesis-gas fermentations. Biotechnology Progress 15, 834–844.
Bouaifi, M., Hebrard, G., Bastoul, D., Roustan, M., 2001. A comparative study of gas hold-up, bubble size, interfacial area and mass transfer coefficients in gas-liquid reactors and bubble columns. Chemical Engineering and Processing 40, 97–111.
References
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Heiskanen, H., Virkajarvi, I., Viikari, L., 2007. The effects of syngas composition on the growth and product formation of Butyribacterium methylotrophicum. Enzyme and Microbial Technology 41, 362–367.
Klasson, K.T., Ackerson, C.M.D., Clausen, E.C., Gaddy, J.L., 1993. Biological conversion of coal and coal-derived synthesis gas. Fuel 72, 1673–1678.
Lee, K.C., Rittmann, B.E., 2001. Applying a novel autohydrogenotrophic hollow-fiber membrane biofilm reactor for denitrification of drinking water. Water Research 36, 2040–2052.
Younesi, H., Najafpour, G., Mohameda, A.R., 2005. Ethanol and acetate production from synthesis gas via fermentation processes using anaerobic bacterium, Clostridium ljungdahlii. Biochemical Engineering Journal 27, 110–119.
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