sus 6592 003 001.p1 ii.pdfeugon broth quality control and recommended incubation conditions:...

36

Selective enrichment of following microorganism Media Positive test organism Page

Soft drinks

Escherichia coli Wallerstein Differential Broth Escherichia coli ATCC 25922 69Coliform M -Endo Coliform Broth Escherichia coli ATCC 25922 50Yeast and Mold M-Green Select Broth Saccharomyces cerevisiae ATCC 9763 53

M-Green Yeast and Mold Saccharomyces cerevisiae ATCC 9763 54Lactobacillus acidophilus Orange Serum Broth Lactobacillus acidophilus ATCC 314 58


Beer and wine

Escherichia coli Wallerstein Differential Broth Escherichia coli ATCC 25922 69Coliform M -Endo Coliform Broth Escherichia coli ATCC 25922 50Yeast and Mold M-Green Select Broth Saccharomyces cerevisiae ATCC 9763 53

Wallerstein Nutrient Broth Saccharomyces cerevisiae ATCC 9763 69


Escherichia coli m-Total Count Media Escherichia coli ATCC 25922MI-Broth Escherichia coli ATCC 25922Tryptic Soy Broth Escherichia coli ATCC 25922M -Endo Coliform Broth Escherichia coli ATCC 25922Total Count Media with TTC Escherichia coli ATCC 25922

Coliform MacConkey with MUG Escherichia coli ATCC 25922 47M-FC/ M-FC Rosolic Acid Escherichia coli ATCC 25922 51

Fecal Streptococci KF-Streptococcus Broth Streptococcus faecalis ATCC 19433 45Pseudomonas aeruginosa Cetrimide Broth Pseudomonas aeruginosa ATCC 10145 39in purified water Pseudomonas Broth Pseudomonas aeruginosa ATCC 10145 60Staphylococci Mannitol Salt Broth Staphylococcus aureus ATCC 25923 48Enterococci Enterococcus Broth Streptococcus faecalis ATCC 19433 42

SUS_6592_036_036_001 07.01.2003 9:39 Uhr Seite 36 BF_0152

5755665065

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Products Media

Quick Media Selection Guide

Quick Media Selection Guide

Dairy products

Escherichia coli Wallerstein Differential Broth Escherichia coli ATCC 25922 69Coliform MacConkey with MUG Escherichia coli ATCC 25922 47Yeast and Mold Potato Dextrose Broth Saccharomyces cerevisiae ATCC 9763 59Lactobacillus MRS Broth Lactobacillus plantarum ATCC 8014 56


Food


Pharmaceutical and cosmetics


Escherichia coli Tryptic Soy Broth Escherichia coli ATCC 25922 66M-Endo Coliform Broth Escherichia coli ATCC 25922 50

Coliform MacConkey with MUG Escherichia coli ATCC 25922 47Fecal Streptococci KF-Streptococcus Broth Streptococcus faecalis ATCC 19433 45Yeast and Mold Sabouraud Dextrose Broth Saccharomyces cerevisiae ATCC 9763 62Staphylococci Mannitol Salt Broth Staphylococcus aureus ATCC 25923 48Pseudomonas aeruginosa Cetrimide Broth Pseudomonas aeruginosa ATCC 10145 39

Pseudomonas Broth Pseudomonas aeruginosa ATCC 10145 60

Escherichia coli Tryptic Soy Broth Escherichia coli ATCC 25922 66MIBlue Escherichia coli ATCC 25922

Coliform MacConkey with MUG Escherichia coli ATCC 25922 47Fecal Streptococci KF-Streptococcus Broth Streptococcus faecalis ATCC 19433 45Yeast and Mold Potato Dextrose Broth Saccharomyces cerevisiae ATCC 9763 59Lactobacillus MRS Broth Lactobacillus plantarum ATCC 8014 56

SUS_6592_037_037_001 07.01.2003 9:39 Uhr Seite 37 BF_0152

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Media Products

Brilliant Green Bile Broth 2 %

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Additional information:

To prevent the growth of accompanying microorganisms in this media anincreased concentration of brilliant green should be added.Salmonella, for example, are not able to ferment either lactose or sucrose. For this reason the lactose contained in this medium allowsidentification of accompanying, weakly lactose-positive or lactose-negativeorganisms.

Order information Brilliant Green Bile Media

Bottled broth 9 ml vial, Durham tube 20 10 496 710

Organisms Characteristics

E. coli ATCC 25922 Growth / gas

E. aerogenes ATCC 13048 Growth / gas

E. faecalis ATCC 29212 Inhibited

S. aureus ATCC 25923 inhibited

Product Description Qty/Pkg Order No

SUS_6592_038_001 18.12.2002 15:57 Uhr Seite 38 Inserat_BC_D_S_03

Products Media

Cetrimide Broth

39

Cetrimide Broth: Pure Culture of Pseudomonasaeruginosa ATCC 10145.

Formulation:Per liter of Water adjusted to pH 7.2 ± 0.2

Peptone 20.0 gMagnesium chloride 1.4 gPotassium sulfate 10.0 gCetrimide 0.3 gGlycerol 10.0 ml

Cetrimide Broth

Quality Control and recommendedincubation conditions:

Positive control:Pseudomonas aeruginosa ATCC 10145, incubated 24–48 hours at 35º C.

Negative control:Escherichia coli ATCC 25922,24–48 hours at 35º C.

Sterility:7 days plated sterility test.

Used for the isolation and determi-nation of Pseudomonas aeruginosa.Cetrimide Broth complies with therecommendations of the United Sta-tes Pharmacopeia and also EuropeanPharmacopeia. The formulation ofthis medium corresponds to thespecifications in the DIN Norm 38411.

Description:Pseudomonas aeruginosa is characteri-zed by the production of pyocyanin (ablue green, water soluble, nonfloures-cent, phenazine pigment), which isstimulated by the inclusion of magne-sium chloride and potassium sulfate inthe broth.Cetrimide (N-cetyl-NNN-trimethylammo-nium bromide) is added to inhibitbacteria other than Pseudomonas aeru-ginosa. Its action as a quaternary ammo-nium cationic detergent causes nitrogenand phosphorus to be released frombacterial cells other than Pseudomonasaeruginosa.

Interpretation:Pyocyanin blue-green pigmentationsurrounding growth is positive forPseudomonas aeruginosa. No colordevelopment is negative for Pseudo-monas aeruginosa.

Historical background:

Harper and Canton followed by Hood described the use of cetrimide (cetyl-tri-methylammonium bromide) for selective isolation of Pseudomonas aeruginosa.Lawbury used cetrimide in a 0.1 % concentration for clinical application.Sawbury and Collins later reported a modification of the concentration ofcetrimide required for selectivity. The introduction of ”Cetevlon“ (cetrimide)stimulated a new study to determine the minimum concentration of cetrimiderequired for selective isolation of Pseudomonas aeruginosa for mixed clinicalflora. The new experiments demonstrated that a concentration of 0.03 % usingthe much improved Cetavlon was sufficient for selectivity.Brown and Sawbury introduced the use of a new improved cetrimide agar in1965. By combining the Medium B of King, Ward and Raney with the 0.03 %cetrimide concentration previously introduced, they developed a medium thatwould support the grow of most desired organisms.

Order information Cetrimide Broth


Ampouled Media 2 ml 50 10 496 146Bottled broth 50 ml 8 10 496 856*Petri dishes with sterile pads 47 mm 100 10 498 544Petri dishes with sterile pads 50 mm 50 10 445 905

* available on request

Organisms Characteristics Coloring

P. aeruginosa ATCC 10145 Growth Blue/green

E. coli ATCC 25922 inhibited

S. aureus ATCC 25923 inhibited


Media Products

EC Broth

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EC Broth was developed by Hajna and Perry for use in the detection ofcoliform bacteria at 37° C and Escherichia coli at 44.5° C. This bufferedlactose broth was designed to improve the methods of detection ofcontamination of waters, milk and shellfish. Bile salts were incorporated toinhibit the growth of gram-positive cocci and sporeformers whichfrequently were responsible for false-positive results obtained when usinglactose broth or lauryl tryptose broth. The EC Broth formula conforms tothat recommended by the APHA for use in determining the source of waterpollution. Through employment of the elevated temperature confirmatorytest procedure, differentiation can be made between coliforms of fecalorigin (intestine of warm-blooded animals) and coliforms from othersources

Order information EC Media

Bottled broth 9 ml, vials, Durham tubes 20 10 496 714


Organisms Growth at 44.5º C


E. aerogenes ATCC 13048 Growth / no gas

E. faecalis ATCC 29212 inhibited

Organisms Growth at 37º C


E. aerogenes ATCC 13048 Growth / gas



Products Media

EC Broth with MUG

Quality Control and recommendedincubation conditions:Positive control:Escherichia coli ATCC 25922,incubated 24 hours at 35–37º C

Negative control:Enterobacter aerogenes ATCC 13048,incubated 24 hours at 35–37º C.


Used for the detection of Escherichiacoli in water and food samples by afluorogenic procedure.

Description:The presence of the fluorescence usinga long-wave UV light source confirmsthe presence of Escherichia coli, andany further confirmation is not required.MUG detects anaerogenic strains,which may not be detected in theconventional procedure.Lactose is a source of energy. Caseinpeptone provides additional nutrients.The mixture of bile salts is inhibiting forgram-positive bacteria, particularlybacilli and fecal streptococci.The substrate 4-methylumbelliferyl-β-D-glucuronide is hydrolyzed by anenzyme, β-glucuronidase, possessedby most Escherichia coli and a fewstrains of Salmonella, Shigella andYersinia, to produce a fluorescent endproduct, 4-methylumbelliferone.

Interpretation:The presence of Escherichia coli isdetected by the appearance offluorescence throughout the tube.

EC Broth with MUG

Formulation:Per liter of water adjustedto pH 6.9 ± 0.2

Pancreatic Digest of Casein 20.0 gLactose 5.0 gBile Salts Mixture 1.5 gDipotassium Phosphate 4.0 gMonopotassium Phosphate 1.5 gSodium Chloride 5.0 g4-Methylumbelliferyl-β-D-glucuronide 50 mg

EC-Broth: Vial left: Control; Vial right: Broth inocu-lated with Escherichia coli ATCC 25922.

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Order information EC Media with MUG


Bottled broth 9 ml, vial 20 10 496 709Bottled broth 9 ml vial, no Durham Tubes 20 10 496 332

Organisms Growth at 44,5° C

E. coli ATCC 25922 Growth/gas/ fluorescence

E. aerogenes ATCC 13048 inhibited fluorescence


Organisms Growth at 35° C

E. coli ATCC 25922 Growth/gas/ fluorescence

E. aerogenes ATCC 13048 Growth/gas/ no fluorescence


SUS_6592_041_041_001 07.01.2003 9:40 Uhr Seite 41 BF_0152

Media Products

Enterococcus Broth

Quality Control and recommendedincubation conditions:Positive control.Enterococcus faecalis ATCC 19433,incubated at 35º C for 24 hours.

Negative control:Escherichia coli ATCC 25922incubated at 35º C for 24–48 hours.

Sterility test:14 days plated sterility test.

Selected media for use in membranefiltration procedures for the isolationand enumeration of enterococci infood, water and other materials.

Description:Enterococcus broth is a modified ver-sion of the improved media describedby Slanetz and Bartley with TTC. Themembrane filtration method is simple toperform, does not require confirmationand permits a direct count of ente-rococci in 48 hours.

Interpretation:Enterococci appear as pink to darkmaroon colonies from 0.5–3 mm in dia-meter.

Enterococcus Broth

Formulation:Per liter of water adjusted to pH 7.2 ± 0.2

Yeast extract 5.0 g Casein 15.0 gDextrose 2.0 gPapaic digest of soybean meal 5.0 gPotassium phosphate 4.0 gSodium azide 0.4 gTriphenyltetrazoliumchloride1% 10 ml

Enterococcus Broth: A pure culture of Enterococ-cus faecalis ATCC 19433 appears pink to red onthis media.

42

Additional informations:

The presence of sodium azide function as inhibitor for the growth of theentire accompanying Gram-negative microbial flora. As described aboveEnterococci reduce TTC and therefore, their colonies appear pink to darkmaroon in color. Furthermore an improved selectivity for enterococci canbe obtained by adding additives like carbonate and Tween 80® to themedia (Lachica and Hartman,1968).

Order information Enterococcus Media

Ampouled Media 2 ml 50 10 496 120Petri dishes with sterile pads 47 mm 100 10 498 544Petri dishes with sterile pads 50 mm 50 10 445 905



Organism Growth/Coloring

E. faecalis ATCC 19433 Pink to red colonies

E. coli ATCC 25922 Inhibited

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Products Media

Eugon Broth

Quality Control and recommendedincubation conditions:Positive control:Escherichia coli ATCC 25922,24–48 hours at 35–37º CCandida albicans ATCC 10231,48 hours at 25–30º C

Negative control:Not undertaken.


Used for the cultivation of a widevariety of microorganisms, includingfastidious bacterial species.

Description:Eugon media was developed to obtaineugonic (luxuriant) growth of fastidiousmicroorganisms. The unenriched mediasupports rapid growth of lactobacilliassociated with cured meat products,dairy products and other food.The high concentration of Dextrose isthe energy source for rapid growth ofbacteria. L-cystine and sodium sulfiteare added to stimulate growth. Sodiumchloride maintains the osmotic balanceof the media. The high carbohydratecontent along with high sulfur (cystine)content improves growth with chromo-genicity.

Interpretation:Colony morphology and color are spe-cies specific.

Eugon Broth


Pancreatic Digest of Casein 15.0 gPapaic Digest of Soybean Meal 5.0 gSodium Chloride 4.0 gl-Cystine 0.3 gSodium Sulfite 0.2 gDextrose 5.5 g

Eugon Broth: A pure culture of Escherichia coliATCC 25922 appears on Eugon Broth with typicalwhite-creamy to opaque colonies.

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Organism Coloring

E. coli ATCC 25922 White, cream to opaquecolonies

Order information Eugon Media





Media Products

HPC Broth with TTC

Quality Control and recommendedincubation conditions:Positive control:Escherichia coli ATCC 25922,incubate for 24–48 hours at 35º C.



Used for heterotrophic plate countsin the examination, for example, ofpotable water, dairy products andswimming pools.

Description:HPC is used to determine total count atincubation temperatures of 35º C. Allbacteria develop on HPC with indicatormedia and produce a red color as aresult of the precipitation of formazanfollowing the reduction of 2,3,5-triphenyltetrazolium chloride (TTC) bybacteria.

Interpretation:Colonies that develop on HPC arecounted as total heterotrophic counts.Specific colony identification should bedone using standard microbiologicalidentification techniques.

HPC Broth with TTC


Peptone 30.0 gGelatin 15.0 gGlycerol 15.0 mlTTC, 1% solution 10.0 ml

HPC with Indicator: Escherichia coli ATCC 25922shows a typical red to pink coloring.

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Order information HPC with TTC





E. coli ATCC 25922 Growth Pink to red

E. faecalis ATCC 29212 Growth Pink to red

S. aureus ATCC 25923 Growth Pink to red

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Products Media

KF-Streptococcus Broth

Quality Control and recommendedincubation conditions:Positive control:Enterococcus faecalis ATCC 19433,incubated 24–48 hours at 35° C.

Negative control:Escherichia coli ATCC 25922,incubated 24– 48 hours at 35° C.


Selected media for the isolation andenumeration of fecal streptococci

Description:KF-Streptococcus Broth is selective forthe determination of fecal streptococciin polluted surface waters. Maltose andlactose are fermentable carbohydrates,sodium azide is the selective agent andbrom cresol purple is the indicator dye.

Interpretation:Identification of fecal streptococci hasto be undertaken using conventionalmicrobiology techniques.

KF-Streptococcus Broth


Peptone 10.0 gYeast extract 10.0 gSodium chloride 5.0 gSodium glycerophosphate 10.0 gMaltose 20.0 gLactose 1.0 gSodium azide 0.4 gBrom cresol purple 15 mgTTC 1% solution 10.0 ml

KF-Streptococcus Broth: Pure culture of Entero-coccus faecalis ATCC 19433 develops on thismedia a typical red colony coloring.

Order information KF-Streptococcus Media


Ampouled Media 2 ml 50 10 496 125Bottled broth 50 ml 8 10 496 753*

100 ml 1 10 496 754*500 ml 1 10 496 755*

Petri dishes with sterile pads 47 mm 100 10 498 544Petri dishes with sterile pads 50 mm 50 10 445 905



E. faecalis ATCC 29212 Growth Red

E. faecalis ATCC 19433 Growth Red

E. aerogenes ATCC 13048 inhibited



Order information Lauryl Sulfate or Lauryl Tryptose Media

Lauryl Sulfate or Lauryl Tryptose Broth 9 ml vials 20 10 496 722Petri dishes with sterile pads 47 mm 100 10 498 544Petri dishes with sterile pads 50 mm 50 10 445 905



Media Products

Lauryl Sulfate or Lauryl Tryptose Broth

Quality Control and recommendedincubation conditions:Positive control:Escherichia coli ATCC 29522incubation for 24–48 hours at 35º C.

Negative control:Enterococcus faecalis ATCC 19433incubated at 35º C for 24–48 hours = no growth.

Sterility test:7 days plated sterility test.

Used for the detection of coliformorganisms in water, wastewater andfoods.

Description:This media was developed for thedetection of coliform organisms by theAmerican Public Health Association(APHA). It is now the standard mediumof choice in the presumptive phase ofthe standard coliform MPN test for themicrobiological examination of water.

Interpretation:Lactose acts as a source of fermen-table carbohydrates for coliforms. Thefermentation of lactose with gas for-mation is a presumptive test for coli-forms. Sodium lauryl sulfate inhibits thegrowth of organisms other than coli-forms.

Lauryl Sulfate or Lauryl Tryptose Broth


Sodium lauryl sulfate 0.1 g Pancreatic Digest of Casein 20.0 gLactose 5.0 gDipotassium phosphate 2.75 gMonopotassium phosphate 2.75 gSodium chloride 5.0 g

Lauryl Sulfate Broth: Pure culture of Escherichiacoli ATCC 25922 cultivated on this media showsnormal growth with gas formation.

46


E. coli ATCC 25922 Growth/gas

E. aerogenes ATCC 13048 Growth/gas

S. faecalis ATCC 29212 inhibited


Order information MacConkey with MUG




Products Media

MacConkey with MUG

Quality Control and recommendedincubation conditions:Positive control:Escherichia coli ATCC 25922,incubated at 35º C for 24 hours.Checked for florescence at 366 nm.


Sterility.7 days plated sterility test.

Used in the presumptive phase forthe presence of coliform bacteria inwater and foods. Selective to thedetection of gram-negative lactosefermenting bacilli. This mediumlargely complies with the EuropeanPharmacopeia.

Description:MacConkey Broth was originallydeveloped by MacCONKEY and HILL(1901) and the formulation for the agarwas modified by MacConkey in 1950. MacConkey developed this medium forthe cultivation of enteric pathogens andcoliforms. It contains bile salts, whichinhibit certain gram-negative bacteria,and crystal violet, which inhibits gram-positive organisms such as enterococciand staphylococci. Combination of thelactose and neutral red indicator toproduce pink to red colored coloniesindicates an isolate with the ability toferment lactose. Non-lactose fermen-ting organisms are colorless. The ad-dition of MUG (4-Methylumbellifryl-β-D-glucuronide), which is a fluorogenicenzyme, allows the media to selectivelyidentify Escherichia coli. MUG ishydrolyzed by the Escherichia colispecific enzyme β-glucuronidase to re-lease 4-Methylumbellifone which fluo-resces under ultraviolet light.

Interpretation:Fluorescence under UV light is specificfor the presence of Escherichia coli.Lactose fermenting organisms grow aspink to red colonies.

MacConkey with MUG

Formulation:Make to 1 liter and adjust pH to 7.1

Casein Peptone 17.0 gProteose Peptone 3.0 gLactose 10.0gBile Salts #3 1.5 gSodium Chloride 5.0 gNeutral Red 30 mgCrystal Violet 0.1 mgMUG (4-Methylumbelliferyl-β-D-glucuronide) 0.1 g

MacConkey with MUG: Escherichia coli ATCC25922 forms as lactose-fermenting organisms pinkto red colonies.

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S. typhimurium ATCC 14028 Growth Clear

E. coli ATCC 25922 Growth/fluorescence

Additional information: MacConkey Broth is a modification of the original bile salt broth where bromocresol purple is used in place of neutral red or litmus as the indicator. Bile saltsreplace 0.5 % sodium taurocholate in the origin formulation. The addition of MUG(4-Methylumbellifryl-(-D-glucuronide), which is a fluorogenic enzyme, allows themedia to selectively identify Escherichia coli. MUG is hydrolyzed by theEscherichia coli specific enzyme (β-glucuronidase to release 4-Methylumbellifonewhich fluoresces under ultraviolet light).

Historical background:MacConkey Broth is a modification of the formula given by MacConkey whichcorresponds to the alternative formulation recommended by the World HealthOrganization. The medium is used for the presumptive determination of thepresence of coliform organisms (gram-negative, lactose – fermenting bacilli) inwater and milk as well as other materials. Presence of lactose – fermentingorganisms is detected by the change of color of the medium (from purple toyellow) after inoculation and incubation.The original formula of MacConkey calledfor use of litmus as an indicator of acid reaction. In later investigations neutral redwas found to be a more suitable indicator. More recently, Childe and Allendemonstrated an inhibitory effect on the growth of Escherichia coli by neutral redin this medium. Bromcresol purple is not only less inhibitory, but also gives amore clear – cut indication of acid reaction.


Media Products

Mannitol Salt Broth

Quality Control and recommendedincubation conditions:Positive control:Staphylococcus aureus ATCC 25923,24–48 hours at 35º C.

Negative control:Escherichia coli ATCC 25922,24–48 hours at 35º C.


Used for the selective isolation andenumeration of staphylococci. Inaddition the medium complies withthe recommendations in the UnitedStates Pharmacopeia.

Description:Because of the amount of peptonesand beef extract, Mannitol Salt is anutrient rich medium. Most bacteria(other than staphylococci) are inhibitedby the high concentration of sodiumchloride. Organisms capable of fer-menting mannitol e.g. Staphylococcusaureus, cause a pH change in themedia. With phenol red as the pHindicator the colonies appear with ayellow coloration.

Interpretation:Typical pathogenic staphylococciferment mannitol and form yellowcolonies with yellow zones, whiletypical non-pathogenic staphylococcido not ferment mannitol and form redcolonies.

Mannitol Salt Broth


Beef Extract 1.0 gPancreatic Digest of Casein 5.0 gPeptic Digest of Animal Tissue 5.0 gSodium Chloride 75.0 gd-Mannitol 10.0 gPhenol Red 25 mg

Mannitol Salt Broth: Staphylococcus aureus ATCC25923 forms typical yellow colonies with zones onthis media. Indication for Mannitol positiveorganisms.

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The tolerance of Staphylococcus aureus to high concentrations of sodiumchloride was reported by Koch in 1942. In 1945, Chapman described aformulation which incorporated 7.5 % sodium chloride in phenol redmannitol agar to successfully cultivate pathogenic staphylococci. Thesecoagulase positive organisms grew in large colonies surrounded by yellowzones. Non-pathogenic staphylococci are usually less luxuriant after the 36hour incubation period recommended by Chapman.Mannitol Salt Broth and Agar is recommended for the enumeration ofstaphylococci in food and dairy products by the American Public HealthAssociation.

Order information Mannitol Salt Media





S. aureus ATCC 25923 Growth Yellow with yellow zones

S. epidermidis ATCC 12228 Growth Red with no zone


E. aerogenes ATCC 13048 inhibited

Appearance of Colonies Microorganisms

Surrounded by bright Mannitol-positive:yellow zones, abundant S. aureusgrowth

pink to red colonies Mannitol-negative:growth is usually poorer S. epidermis and others


To use membrane lauryl sulphate brothfor the identification of thermotolerantcoliforms incubate at 30º C for 4 hoursthen incubate for further 14 hours at44º C. Yellow colonies are counted aspresumptive coliforms which requireconfirmation.

Quality Control and recommendedincubation conditions:Positive control:Escherichia coli ATCC 25922incubation for 4 hours at 30º Cthen for 14 hours at 37º C.



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Products Media

Membrane Lauryl Sulfate Broth

For the presumptive identification ofcoliforms and Escherichia coli in waterand drinking water. Prepared to theformula published in Journal ofHygiene (PHLS/SCA).

Description:This media was developed for thedetection of coliform organisms and isnow the media of choice for theenumeration of total coliforms andEscherichia coli in the United Kingdom.This media replaced membrane enrichedbroth containing 0.4 % Teepol 610.

Interpretation:Lactose acts as a source of ferment-able carbohydrates for coliforms. Phenolred acts as an indicator of acidity as aresult of coliform metabolism. Incubatefor 4 hours at 30º C then increase thetemperature to 37º C and incubate forfurther 14 hours. Yellow colonies arecounted as presumptive coliforms whichrequire confirmation. Pink or colorlesscolonies are not counted as coliforms.

Membrane Lauryl Sulfate Broth


Peptone 40.0 gYeast extract 6.0 gLactose 30.0 gPhenol red (0.4% solution) 50.0 mlSodium lauryl sulfate 1.0 g

This picture shows a mix culture on MembraneLauryl Sulphate Broth incubated at 37° C. Orga-nisms like Escherichia coli ATCC 25922 and Ente-robacter aerogenes ATCC 13048 form yellowcolonies whereas Enterococcus faecalis ATCC29212 appears as pink colonies.

Order information Membrane Lauryl Sulfate Media




Organisms Characteristics Coloring 37 °C

E. coli ATCC 25922 Growth Yellow

E. aerogenes ATCC 13048 Growth Yellow

E. faecalis ATCC 29212 Growth Pink or colorless

Organisms Characteristics Coloring 44 °C

E. coli ATCC 25922 Growth Yellow

E. aerogenes ATCC 13048 Inhibited Inhibited

E. faecalis ATCC 29212 Inhibited Inhibited


Media Products

M-Endo Coliform Broth

Quality Control and recommendedincubation conditions:Positive control:Escherichia coli ATCC 25922, 24 hours at 35º C.

Negative control:Pseudomonas aeruginosa ATCC 10145,24 hours at 35º C.


M-Endo Broth is used for theenumeration of coliforms by mem-brane filtration. It is used to dif-ferentiate lactose from non-lactosefermenting intestinal organisms andas a presumptive test for coliforms.

Description:M-Endo is a red colored media, whichneeds to be stored in the dark to preventdiscoloration of the media. Gram-positivebacteria are inhibited on this media bythe desoxycholate and lauryl sulfate. Theaddition of ethanol increases theantibacterial nature of the formulation.Lactose fermenting organisms formaldehydes, which react with Schiffsreagent (basic fuchsin and sodium sulfite)to give red colored zones around thecolonies. Coliform colonies are thereforered with a characteristic metallic sheen.

Interpretation:Production of both acid and aldehydeby lactose fermenters, such asEscherichia coli, produce deep redcolonies that color the surroundingmedium and have a green metallicsheen. Non-lactose fermenters formcolorless, translucent colonies.

M-Endo Coliform Broth


Peptones 10.0 g(equal parts digests of animal tissueand casein)Peptic Digest of Animal Tissue 5.0 gPancreatic Digest of Casein 5.0 gYeast extract 1.5 gLactose 12.5 gSodium chloride 5.0 gDipotassium phosphate 4.375 gMonopotassium phosphate 1.375 gSodium lauryl sulfate 50 mgSodium desoxycholate 0.1 gSodium sulfite 2.1 gBasic fuchsin 1.05 g95 % Ethanol 30.0 ml

M-Endo Broth: Mixed culture of Escherichia coliATCC 25922: (red colonies with green metallicsheen) and Pseudomonas aeruginosa ATCC 10145(pink to colorless).

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Order information M-Endo

Ampouled Media 2 ml 50 10 496 103Bottled broth 50 ml, Screw cap 8 10 496 700

50 ml, Septa cap 8 10 496 701Petri dishes with sterile pads 47 mm 100 10 498 544Petri dishes with sterile pads 50 mm 50 10 445 905




E. coli ATCC 25922 Growth Red with greenmetallicsheen

E. aerogenes ATCC 13048 Growth Red colonieswith orwithoutgreenmetallicsheen

P. aeruginosa ATCC 10145 Growth Pink to colorless

S. aureus ATCC 25923 Marked to complete inhibition



100 ml 1 10 496 757*500 ml 1 10 496 758*



Products Media

M-FC Broth

Quality Control and recommendedincubation conditions:Positive control:Escherichia coli ATCC 25922,incubated 24 hours at 44.5º C.

Negative control:Enterobacter aerogenes ATCC 13048,incubated 24 hours at 44.5º C.


M-FC media (membrane FecalColiform media) is used for the de-tection of fecal coliforms as an indexof water pollution.

Description:Allows the development of fecalcoliforms at elevated temperatures(44.5° C).

Interpretation:Bile salts included in the medium inhibitthe growth of gram-positive bacteria.Fecal coliforms ferment lactose atelevated temperatures and produceblue colonies. Other organisms formgrey to cream colonies.

M-FC Broth


Tryptose 10.0 gPeptone No. 3 3 5.0 gYeast extract 3.0 gSodium chloride 5.0 gLactose 12.5 gBile salts 1.5 gAniline blue 0.1 g

M-FC-Media: A pure culture of Escherichia coliATCC 25922 shows a typical blue coloring on m-FC Broth.

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M-FC-Media: A cultivated mix culture indicateslactose fermenters as blue colonies whereas non-lactose fermenters form grey to creamy coloniese.g. Enterobacter aerogenes ATCC 13048.Order information M-FC-Media



E. coli ATCC 25922 Growth Blue

E. aerogenes ATCC 13048 Growth Gray to cream



Media Products

M-FC with Rosolic Acid

Quality Control and recommendedincubation conditions:Positive control:Escherichia coli ATCC 25922,24 hours at 44,5º C.

Negative control:Enterobacter aerogenes ATCC 13048,24 hours at 44.5º C.


M-FC Broth with Rosolic Acid is usedfor the detection of fecal coliforms bythe membrane filtration technique.

Description:M-FC with Rosolic Acid acts andfunctions in the same way as m-FCBroth. Rosolic acid inhibits bacterialgrowth in general, except for fecalcoliforms.

Interpretation:Bile salts inhibit non-enteric bacteria.Aniline blue indicates the ability of fecalcoliforms to ferment lactose to acidthat causes a pH change in themedium. Other organisms form grey tocream colonies.

M-FC with Rosolic Acid

Formulation:Per liter of water adjusted to pH 7.4

Tryptose 10.0 gPeptone No. 3 5.0 gYeast extract 3.0 gSodium chloride 5.0 gLactose 12.5 gBile salts 1.5 gAniline blue 0.1 gRosolic Acid 1% 10.0 ml

M-FC with Rosolic Acid: Escherichia coli ATCC25922 forms blue colonies whereas non-lactosefermenters appears as grey colonies.

52


Fecal coliform (FC) Medium for the membrane filtration technique wasdescribed by Geldereich et al. in 1965. It was the first membrane filtrationtechnique to be incubated at 44.5 ± 0.2.

Order information m-FC with Rosolic Acid

Ampouled Media 2 ml 50 10 496 114Bottled broth 50 ml 8 10 496 719* Petri dishes with sterile pads 47 mm 100 10 498 544Petri dishes with sterile pads 50 mm 50 10 445 905




E. coli ATCC 25922 Growth Blue

E. aerogenes ATCC 13048 Growth Gray to cream



Quality Control and recommendedincubation conditions:Positive control:Candida albicans ATCC 10231,incubated 48 hours at 25–30º C.

Negative control:Escherichia coli ATCC 25922,incubated 48 hours at 35º C.


M-Green Select Broth

M-Green Select Broth


E. coli ATCC 25922 Partial to marked inhibition

S. cerevisiae ATCC 4098 Growth

C. albicans ATCC 10231 Growth

Products Media

Used for the enumeration of yeastsand mold in soft drinks and fruitjuices.

Description:M-Green Select Broth is an improvedmodification of the liquid medium, m-Green Yeast and Mold Broth and wasdeveloped to improve efficiency ofdetection and enumeration of fungi insugar based drinks using the mem-brane filtration method.This medium has a low pH, whichinhibits bacterial growth. The additionof Chloramphenicol further inhibits thegrowth of bacteria to allow for the de-velopment and enumeration of yeastand mold.The addition of bromocresol green,which diffuses into fungal colonies asan alkaline reaction, allows them to beeasily identified. Metabolic by-productsfrom the developing colonies diffuseinto the surrounding medium, furtherreducing the pH which aids in theinhibition of bacterial growth, but alsoproduces an acid reaction whichcauses residual bromocresol green tochange to yellow.

Interpretation:Green opaque colonies against ayellow background indicative of thegrowth of yeast. Mold colonies aregreen and filamentous.


Dipeptone 10.0 gYeast extract 9.0 gDextrose 50.0 gMagnesium sulfate 2.1 gPotassium phosphate 2.0 gDiastase 50 mgThiamine 50 mgBromocresol green 26 mgChloramphenicol, 1% Solution 8.5 ml

M-Green Select Media: Ideal for the enumerationof yeasts & mold.

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Order information M-Green Select Media





Media Products

M-Green Yeast and Mold

Quality Control and recommendedincubation conditions:Positive control:Candida albicans ATCC 10231,48 hours at 25–30º C.



Used for the enumeration of yeastand mold in soft drinks and fruitjuices.

Description:M-Green is an improved modification ofthe liquid medium, m-Yeast and MoldBroth and was developed to improveefficiency of detection and enumerationof fungi in sugar based drinks using themembrane filtration method.This medium has a low pH, whichinhibits bacterial growth. The additionof bromocresol green, which diffusesinto fungal colonies as an alkalinereaction, allows them to be easilyidentified. Metabolic by-products fromthe developing colonies diffuse into thesurrounding medium, further reducingthe pH which aids in the inhibition ofbacterial growth, but also produces anacid reaction which causes residualbromocresol green to change to yellow.

Interpretation:Green opaque colonies against ayellow background are indicative of thegrowth of yeast. Mold colonies aregreen and filamentous. Examples ofdetected organisms:

M-Green Yeast and Mold


Dipeptone 10.0 gYeast extract 9.0 g Dextrose 50.0 gMagnesium sulfate 2.1 gPotassium phosphate 2.0 gDiastase 50 mgThiamine 50 mgBromocresol green 26 mg

M-Green Yeast & Mold Broth: Typical growth ofCandida albicans ATCC 10231 on a blackmembrane.

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Order information M-Green Yeast & Mold


100 ml 1 10 496 760*500 ml 1 10 496 761*

Bottled agar 100 ml 1 10 496 705Petri dishes with sterile pads 47 mm 100 10 498 544Petri dishes with sterile pads 50 mm 50 10 445 905




Products Media

MI Broth and MI Agar

Interpretation:Fluorescent blue colonies areEscherichia coli.Colonies that demonstrate blue/whitefluorescence where the colonies areclear, cream or pale yellow in color areother coliform organisms. Clearcolonies that do not fluoresce are non-coliforms.

Quality Control and recommendedincubation conditions:Positive control:Escherichia coli ATCC 25922,18–24 hours at 35º C.Enterobacter aerogenes ATCC 13048,18–24 hours at 35º C.

Negative control:Pseudomonas aeruginosa ATCC 10145,24 hours at 35º C.


Used for the simultaneous detectionof total coliforms and Escherichiacoli in water according to the SurfaceWater Treatment Rule (USEPA) andthe Total Coliform Rule (USEPA)

Description:MI Broth detects the presence ofcoliform bacteria by the production ofβ-galactosidase, which cleaves thesubstrate MUGal to produce 4-Methyl-umbelliferone, which fluoresces onexposure to UV light. Non-coliforms donot produce this enzyme and thereforedo not fluoresce on the medium.Escherichia coli is detected by thecompound IBDG. The β-glucuronidaseproduced by Escherichia coli cleavesthe substrate to produce a blue indigocolor in the colonies. As Escherichiacoli is also a total coliform, and alsoproduces β-galactosidase it will alsofluoresce.The antibiotic cefsulodin is added toinhibit the growth of gram-positivebacteria and some non-coliform gram-negative bacteria that can cause falsepositive reactions.

MIBlue is specially developed for thefood industry.

MI Broth and MI Agar


Protease peptone 5.0 gYeast extract 3.0 gβ-d-lactose 1.0 gMUGal 0.1 gNaCl 7.5 g K2HPO4 3.3 gKH2PO4 1.0 gSodium lauryl sulfate 0.2 gSodium desoxycholate 0.1 gIBDG 0.32 gCefsulodin 5 mg(Agar) 15.0 g

MI-Media: Pure Culture of Escherichia coli ATCC25922 with UV light.

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MI-Broth Media: Mixed Culture with Escherichiacoli ATCC 25922 and Enterobacter aerogenesATCC 13048 without UV.Order information MI Media


Ampouled Media 2 ml 50 10 496 191Bottled broth 50 ml 1 10 496 851Bottled agar 50 ml 1 10 496 847Petri dishes with sterile pads 47 mm 100 10 498 544Petri dishes with sterile pads 50 mm 50 10 445 905MIBlue 10 496 501*



E. coli ATCC 25922 Growth Blue with fluorescence

E. aerogenes ATCC 13048 Growth Yellow withfluorescence

P. aeruginosa ATCC 10145 Complete inhibition


Order information MRS Media




Media Products

MRS Broth

Quality Control and recommendedincubation conditions:Positive control:Lactobacillus plantarum ATCC 8014Lactobacillus fermentum ATCC 9338incubated at 35º C for 48–72 hours.



Used for the isolation and cultivationof lactobacilli. MRS Broth complieswith the German DIN-Norm 10109and the International Standard ISO13721 for the detection of lactoseinmeat and to the regulations acc. to §35 LMBG (06.00/35) for the detectionin meat.

Description:MRS medium supports luxuriantgrowth of all lactobacilli, even the slowgrowing species.

Interpretation:Lactobaccili appear as white colonies.Pediococcus and Leuconostoc mayalso grow on MRS.

MRS Broth


Pancreatic Digest of Casein 10.0 gBeef extract 8.0 gYeast extract 4.0 gDextrose 20.0 gDipotassium Phosphate 2.0 gPolysorbate 80 1.0 gAmmonium citrate 2.0 gSodium acetate 5.0 gMagnesium sulfate 0.2 gManganese sulfate 50 mg

MRS Media; Pure Culture of Lactobacillus planta-rum ATCC 8014.

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Additional information:The media contains special growth factors for lactobacilli like polysorbate,acetate, magnesium and manganese. These compounds are known as richnutrient base. On a very low degree of selectivity, Pediococcus and Leu-conostoc species and other secondary bacteria can be cultivated.

Historical background:MRS Agar was developed by de Man, Rogosa & Sharper for the cultivationof Lactobacillus species. The medium will effectively support the growth ofmany strains of lactobacilli that do not generally grow as well as othermedia designed for this purpose. Due to the nutritional factors and theneutral pH other organisms which are not fastidious will grow on themedium.Lactobacillus will grow well on the surface of the medium as well as indeep culture preparations. Although enrichment with carbon dioxide isunnecessary with this medium, the atmosphere must be kept fairly moist

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Order information M-TGE Total Count



100 ml 1 10 496 763*300 ml 1 10 496 764*500 ml 1 10 496 765*



Products Media

M – TGE Total Count Media

Quality Control and recommendedincubation conditions:Positive control:Escherichia coli ATCC 25922,48 hours at 35º C.



Used for the non-selective develop-ment and enumeration of all aerobicbacteria.

Description:All bacteria develop on TGE media andproduce a range of different coloredand sized colonies.

Interpretation:Identification of bacteria should beundertaken by using traditional micro-biology techniques following initialcolony development.For quality control two typicalorganisms are detected and enume-rated with m – TGE Total Count Media:

M – TGE Total Count Media

Formulation:Per liter of water and adjusted to pH 7.0 ± 0.2

Pancreatic Digest of Casein 10.0 gYeast extract 5.0 gDextrose 2.0 g

Pure culture of Escherichia coli ATCC 25922 onm-TGE Total Count Media.

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M-TGE Total Count Media with a mixed culture ofEscherichia coli ATCC 25922 and Staphylococcusaureus ATCC 25923.


E. coli ATCC 25922 Growth Yellow to cream

S. aureus ATCC 25923 Growth Yellow to cream


Order information Orange Serum

Ampouled Media 2 ml 50 10 496 104Bottled Agar 100 ml 1 10 496 713Petri dishes with sterile pads 47 mm 100 10 498 544Petri dishes with sterile pads 50 mm 50 10 445 905



Media Products

Orange Serum Media

Quality Control and recommendedincubation conditions:Positive control:Lactobacillus fermentum ATCC 9338,48 hours at 35º C.Candida albicans ATCC 10231,48 hours at 25–30º C.



Used for the isolation and enume-ration of organisms associated withthe spoilage of citrus juices.

Description:Organisms known to grow in singlestrength and concentrated juices arelactic acid and acetic acid bacteria andyeast. Lactobacilli, Leuconostoc andyeast have all been identified as spoi-lage organisms by numerous authors.Orange serum at pH 5.4 to 5.6 hasbeen reported to yield maximumcounts of all types of spoilageorganisms in mixed cultures, and insingle culture comparison tests.

Interpretation:The low pH of the test solution usuallyprohibits the development of othermicroorganisms which cannot survivelow pH conditions. Therefore develo-ping colonies are presumed to beproblematic organisms.

Orange Serum Media

Formulation:Per liter of water, adjusted to pH 5.6 ± 0.2

Orange serum 10.0 gYeast extract 3.0 gTryptone 10.0 gDextrose 4.0 gDipotassium phosphate 2.5 g

Orange Serum Media agar is especially selectivefor microorganisms which prefer low pH condi-tions e.g. Lactobacillaceae and some yeast e.g.Candida.

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Products Media

Potato Dextrose Broth and Agar Media

Quality Control and recommendedincubation conditions:Positive control:Candida albicans ATCC 10231,incubated at 25–30º C for 48 hours.



Recommended for culturing andenumerating yeast and mold.Potato Dextrose Broth complies withthe recommendations of the AmericanPublic Health Association for foodand the USP.

Description:Potato Dextrose Broth is recommendedin Standard Methods as the media thatgives the most consistent and highestcounts for the recoveries of yeast andmold in dairy products. The inclusion ofpotato extract encourages the growthand development of fungi. SterileTartaric Acid may be added to low thepH to 3.5 ± 0.2 to further inhibit thegrowth of conflicting bacteria.

Interpretation:Yeast, mold and acid tolerant bacteriagrow well on Potato Dextrose Broth.

Potato Dextrose Broth and Agar Media


Potato infusion 4.0 gDextrose 20.0 g

For agar add:Agar 15.0 g

Potato Dextrose Media: Pure culture of Candidaalbicans ATCC 10231.

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In general carbohydrate and potato infusion promote the growth of yeastand mold. Additionally the low pH value has a positive effect by partiallyinhibiting the growth of accompanying bacterial flora. If the medium is usedfor fungal counts, the pH should be adjusted to approximately 3.5. Fungigrow on this medium to develop typical morphology.


Potato Dextrose Broth is commonly used in slide culture preparations offungi to stimulate sporulation and to enhance growth of poorly sporulatingmycelia. It has been used for cultivation and isolation of mold and yeast indairy and food products as recommended by the American Public HealthAssociation and for maintenance of stock cultures of geophilicdermatophytes.

Order information Potato Dextrose Media



C. albicans ATCC 10231 Growth White, creamy

S. cerevisiae ATCC 4098 Growth White, creamy


100 ml 1 10 496 770*500 ml 1 10 496 771*

Bottled agar 23 ml tube 15 10 496 863*100 ml 1 10 496 731*500 ml 1 10 496 767*

1000 ml 1 10 496 768*Petri dishes with sterile pads 47 mm 100 10 498 544Petri dishes with sterile pads 50 mm 50 10 445 905



Order information Pseudomonas Media


100 ml 1 10 496 776*500 ml 1 10 496 777*

Bottled agar 50 ml 8 10 496 772*100 m 1 10 496 773*500 ml 1 10 496 774*




Media Products

Pseudomonas Broth

Quality Control and recommendedincubation conditions:Positive control:Pseudomonas aeruginosa ATCC 10145,24–48 hours at 35º C.

Negative control:Escherichia coli ATCC 25922,24 hours at 35º C.


Used for the isolation of Pseudo-monas and differentiating Pseudo-monas aeruginosa from other pseu-domonads based on pigment forma-tion.Pseudomonas Broth complies withthe formulation recommendations ofthe United States Pharmacopeia andto the specifications in the DIN Norm38411 (examination of water).

Description:Pseudomonas aeruginosa is characteri-sed by the production of pyocyanin (ablue green, water soluble, nonfloures-cent, phenazine pigment), which isstimulated by the inclusion of magne-sium chloride and potassium sulfate inthe broth. Irgasan, an antimicrobialagent, selectively inhibits gram positiveand gram negative bacteria other thanpseudomonads. Glycerol serves as bothan energy source and helps in thepromotion of pyocyanin.

Interpretation:Development of a green to blue greenpigmentation surrounding colonies ispositive for Pseudomonas aeruginosa.Other pseudomonads develop as clearto amber yellow colonies. Non-pseudo-monads are suppressed.

Pseudomonas Broth


Pancreatic Digest of Casein 20.0 gMagnesium chloride 1.4 gPotassium sulfate 10.0 gIrgasan 0.25 gGlycerol 20.0 ml

Pseudomonas Media: Typical growth of Pseudo-monas aeroginosa ATCC 10145.

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Additional information:Identification of most Pseudomonas strains can be obtained by theirdifferent pigmentation according to the compounds used in the media.Some of the strains can only synthesize pyocyanin, some form onlyfluorescein and others form both pigments.Pseudomonas aeruginosa ATCC 27853 is characterised by the productionof pyocyanin (a blue green, water soluble, nonflourescent, phenazinepigment), which is stimulated by the inclusion of magnesium chloride andpotassium sulfate in the broth. The same Pseudomonas aeruginosa strainappears on Pseudomonas Broth as colonies surrounded by a yellow togreenish-yellow zone when different types of peptone are added andmagnesium chloride and potassium sulphate are omitted.BLAZEVIC et al. (1973), noted that some Pseudomonas aeruginosa strainsatypically appear as pyocyanin-negative, fluorescein-positive and for thatreason it is possible to differentiate them from Pseudomonas fluorescensand Pseudomonas putida. Furthermore BRODSKY and NIXON (1973)showed that cultivation on MacCONKEY agar can be used for a simple andrapid differentiation of these strains.


P. aeroginosa ATCC 10145 Growth Blue to blue-green

P. aeroginosa ATCC 27853 Growth Blue to blue-green


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Order information R2 Media


Ampouled Media 2 ml 50 10 496 161Bottled agar 15 ml tube 15 10 496 724

100 ml 1 10 496 723500 ml 1 10 496 726*



Products Media

R2 Broth and R2 Agar

Quality Control and recommendedincubation conditions:Positive control:Escherichia coli ATCC 25922,incubation for not less than 72 hours at35° C.

Negative control:Not applicable.


Used for Heterotrophic plate countsin the examination of potable water.

Description:R2 broth can be used to determineheterotrophic plate count at 35ºC.When incubated at lower temperatures(25–30º C) for longer periods of 72–96hours it can also be used to recoverenvironmentally stressed organisms, orthose that are chlorine tolerant.

Interpretation:Heterotrophic plate counts are not thesame as standard plate counts and amedia such as TGE should be run incombination with R2.

R2 Broth and R2 Agar


Yeast extract 0.5 gPeptones 0.5 gAcid hydrolysate of casein 0.5 gDextrose 0.5 gSoluble starch 0.5 gDipotassium phosphate 0.3 gMagnesium sulfate (anhydrous) 24 mgSodium pyruvate 0.3 g


Sample of tap water on R2 Media.

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E. coli ATCC 25922 Growth

E. faecalis ATCC 29212 Growth

S. aureus ATCC 25923 Growth


Media Products

Sabouraud Dextrose Broth

Quality Control and recommendedincubation conditions:Positive control:Candida albicans ATCC 10231incubated for 72 hours at 25–30º C.



The medium is used for the quan-titative identification of yeast andmold.

DescriptionPeptone in the media is used as anitrogen source for the development offungi. Dextrose acts as an energysource for the growth of micro-organisms. The low pH is favorable forthe development of fungi, especiallydermatophytes, but at the same timeinhibits the development of conta-minating bacteria from clinical speci-mens.

Interpretation:Growth is interpreted as yeast/mold.Subculturing and species/group spe-cific identification is required.

Sabouraud Dextrose Broth


Peptone 10.0 gDextrose 20.0 g


Sabouraud Dextrose Broth is especially selectivefor yeast and mold due to the low pH conditions.

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Sabouraud Dextrose Broth is a modification of dextrose agar described bySabouraud in 1892 for identification of fungi based on their culturalcharacteristics. The medium depended solely upon its acid pH forsuppression of bacteria. Sabouraud Dextrose Agar, Emmons is amodification with a neutral pH and reduced dextrose concentration.Historically, the inhibitory action of Sabouraud Dextrose Agar onmicroorganisms other than fungi was enhanced by the addition of agentssuch as tellurite, copper sulfate, bile salts, dyes and antibiotics.Sabouraud Dextrose Broth (Fluid Sabouraud Medium) is the liquidcounterpart of the agar prepared according to the formulation specified inthe U.S. Pharmacopoeia and National Formulary for sterility testing ofpharmaceutical products. Used in the quantitative identification of yeastand mold.

Order information Sabouraud Dextrose Media


100 ml 1 10 496 785*500 ml 1 10 496 786*

Bottled agar 50 ml 8 10 496 781*100 ml 1 10 496 782*500 ml 1 10 496 783*





C. albicans ATCC 10231 Growth Off-white, creamy

E. coli ATCC 25922 Growth Off-white, creamy

S. cerevisiae ATCC 9763 Growth Off-white


Products Media

Sabouraud 4 % Dextrose Broth

incubation conditions:Positive control:Candida albicans ATCC 10231,incubated 72 hours at 25–30º C.



Used in the quantitative identificationof yeast, mold and acidophilicmicroorganisms.This culture mediumcomplies with the recommendationsof the United States Pharmacopeiaand the European Pharmacopeia.

Description:Peptone in the media is used as anitrogen source for the development offungi. Dextrose acts as an energysource for the growth of microorga-nisms. The low pH is favorable for thedevelopment of fungi, especially der-matophytes, but at the same timeinhibits the development of conta-minating bacteria from clinical speci-mens.The use of Sabouraud media has beendocumented for establishing the mi-crobial concentration in cosmetics andfor the mycological evaluation of food.

Interpretation:Growth is interpreted as yeast/mold.Sub-culturing and species/groupspecific identification is required.Quality Control and recommended

Sabouraud 4 % Dextrose Broth


Peptone 10.0 gDextrose 40.0 g

Sabouraud 4 % Dextrose Media: Pure culture ofCandida albicans ATCC 10231.

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Order information Sabouraud 4% Dextrose Media







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Media Products

Quality Control and recommendedincubation conditions:Positive control:Escherichia coli ATCC 25922,incubated 48 hours at 35º C.



Used for obtaining microbiologicalplate counts from milk and dairyproducts, foods, water and othermaterials of sanitary importance.The formulation of this mediumfollows the demands of the StandardMethods for the Examination ofWater and Wastewater, the AmericanPublic Health Association, theAmerican Water Works Associationand the Water Pollution ControlFederation in 1992 and the StandardMethods for the Examination of DairyProducts of the American PublicHealth Association (1985).

Description:All bacteria develop on StandardMethods and produce a range ofdifferent colored and sized colonies.

Interpretation:It is not possible using StandardMethods Agar to presumptively identifyany bacteria. Identification can only beundertaken using traditional microbio-logy techniques following initial colonydevelopment.


Pancreatic Digest of Casein 5.0 gYeast extract 2.5 gDextrose 1.0 gAgar 15.0 g

Standard Methods Agar

Standard Methods Agar

Same agar as above but incubated with a mixculture of Escherichia coli ATCC 25922 andStaphylococcus aureus ATCC 25923. Both formyellow to creamy colonies.


Standard Methods Agar is amodification of Bowers andHucker Tryptone Glucose SkimMilk Agar. It was shown by Yaleto be more effective in platecount procedures on milk anddairy products.The American Public HealthAssociation in their ”StandardMethods for the Examination ofDairy Products“ recommendsthe use of Standard MethodsAgar for performance of the”Standard Plate Count“ ondairy products.Standard Methods Agar is thesame formulation as PlateCount Agar recommended bythe APHA for use in the”Standard Plate Count“ onwater.

Standard Methods Agar with membrane filter andincubated with a pure culture of Escherichia coliATCC 25922.

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Order information Standard Methods Medium

Bottled agar 100 ml 1 10 496 706Petri dishes with sterile pads 47 mm 100 10 498 544Petri dishes with sterile pads 50 mm 50 10 445 905




E. coli ATCC 25922 Growth Yellow to cream

S. aureus ATCC 25923 Growth Yellow to cream

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Products Media

Total Count Media with TTC

Quality Control and recommendedincubation conditions:Positive control:Escherichia coli ATCC 25922incubated 24–48 hours at 35° C.


Sterility7 days plated sterility test.

Total Count Media with Indicator isused for the non-selective develop-ment and enumeration of all aerobicbacteria by the membrane filtrationmethod.This media complies with thespecifications given by the APHA forthe examination of water (1992) andfor food (1992).

Description:All bacteria develop on Total CountMedia with indicator and produce a redcolor as a result of the precipitation offormazan following the reduction of2,3,5-triphenyltetrazolium chloride(TTC) by bacteria. The table belowshows typical organisms which can beenumerated with this media:

Interpretation:It is not possible using Total CountMedia with Indicator to presumptivelyidentify any bacteria. Identification canonly be undertaken using traditionalmicrobiology techniques followinginitial colony development.

Total Count Media with TTC


Pancreatic Digest of Casein 10.0 gYeast extract 5.0 gDextrose 2.0 gTTC, 1% solution 10.0 ml

Total Count Media with Indicator: Escherichia coliATCC 25922 and Staphylococcus aureus ATCC25923 can be easily detected according to theirred to pink colonies.

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Order information Total Count Media with Indicator





E. coli ATCC 25922 Growth Pink to red

S. aureus ATCC 25923 Growth Pink to red

P. aeruginosa ATCC 10145 Growth Pink to red

E. faecalis ATCC 29212 Growth Pink to red


Media Products

Trypticase Soy Broth (TSB)

Quality Control and recommendedincubation conditions:Positive controls:Escherichia coli ATCC 25922,incubation for 24–48 hours at 35º C.



Trypticase Soy Broth (TSB) is ageneral-purpose medium used forthe cultivation of most bacteria.

Description:TSB broth is a medium that will supportthe growth of a wide variety of micro-organisms including aerobic, facultativeand anaerobic bacteria and fungi.

Interpretation:When used as a broth adsorbed ontopads TSB will support the developmentof mixed cultures of bacteria and fungi.When used in sterility test applicationsturbidity indicates the presence oforganisms when compared to anuninoculated control.

Trypticase Soy Broth (TSB)


Pancreatic digest of casein 17.0 gPapaic digest of soybean meal 3.0 gSodium chloride 5.0 gDipotassium phosphate 2.5 gDextrose 2.5 g

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Order information Trypticase Soy Media (TSB)

Ampoules 2 ml 50 10 496 160Bottled media 50 ml 8 10 496 791*

100 ml 1 10 496 792*100 ml, Septa Cap 1 10 496 750*100 ml 1 10 496 707*

Bottled agar 23 ml 15 10 496 862*Petri dishes with sterile pads 47 mm 100 10 498 544Petri dishes with sterile pads 50 mm 50 10 445 905



Ready to use Tryptic Soy Broth (USP). Notinoculated.


B. subtilis ATCC 6633 Growth





Products Media

Trypticase Soy Broth – Single Strength

Quality Control and recommendedincubation conditions:Positive control:Escherichia coli ATCC 25922,incubated 24–48 hours at 35º C.



Description:General-purpose medium used inqualitative procedures for the culti-vation of fastidious and non-fastidiousmicroorganisms. Trypticase Soy Broth –Single Strength complies with the de-mands of the DIN Norm 10167 for thedetection of Escherichia coli serotype0157:H7 in foods and FDA-BAM for theisolation of enterohemorrhagic Escheri-chia coli (EHEC). In addition the mediaconforms to the formula of the U.S.Pharmacopoeia.

Interpretation:When used as a broth adsorbed ontopads TSB will support the developmentof mixed cultures of bacteria and fungi.When used in sterility test applicationsturbidity indicates the presence oforganisms when compared to an un-inoculated control.

Trypticase Soy Broth – Single Strength



Trypticase Soy Broth-Single Strength. Notinoculated.

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Trypticase Soy Broth was originally developed for detecting the effec-tiveness of sulfonamides against microorganisms such as pneumococciwithout the addition of serum or blood to the medium. Spink and Hamiltondemonstrated the suitability of the medium for cultivation of aerobic andfacultative microorganisms including Brucella. Garrision and Hedgecockboth used the medium to promote the growth of pathogenic fungi. Theaddition of agar to enhance the growth of anaerobic organisms isdescribed by Mashima and Ellison and Fredette, Auger, and Forget.Sherman and Stark demonstrated that a medium containing 6.5 % NaClcould be used for differentiation of group D enterococci from otherstreptococci.

Order information Trypticase Soy Broth-Single Strength







Bottled broth 10 ml, Vials 20 10 496 742*100 ml 1 10 496 707100 ml, wide mouthed 1 10 496 741*



SUS_6592_067_067_001.01 07.01.2003 9:51 Uhr Seite 67

Media Products

Trypticase Soy Broth – Double Strength

Quality Control and recommendedincubation conditions:Positive controls:Escherichia coli ATCC 25922,incubated 24–48 hours at 35º C.



General-purpose medium used inqualitative procedures for the culti-vation of fastidious and non-fastidiousmicroorganisms. This media is alsorecommended for sterility testaccording to U.S. Pharmacopeia.

Description:TSB broth is a medium that will supportthe growth of a wide variety ofmicroorganisms including aerobic,facultative and anaerobic bacteria andfungi.

Interpretation:When used as a broth adsorbed ontopads TSB will support the developmentof mixed cultures of bacteria and fungi.When used in sterility test applicationsturbidity indicates the presence oforganisms when compared to anuninoculated control.

Trypticase Soy Broth – Double Strength



Trypticase Soy Broth (Double Strength). Notinoculated.

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Order information Trypticase Soy Broth (Double Strength)

Bottled broth 50 ml, W/M bottle 1 10 496 725100 ml 1 10 496 708


Trypticase Soy Broth (Double Strength): left vial:

control, right vial inoculated with Escherichia coli

ATCC 25922.







Products Media

Interpretation WL Differential Broth:When incubated at pH 5.5 estimates ofbeer cocci and lactic rods can be madeunder anaerobic conditions. Estimatesof acetic acid rods and thermobacteriaare made under aerobic conditions.

WL Differential Broth

Quality Control and recommendedincubation conditions WL Nutrient:Positive control:Sacchromyces cerivisiae ATCC 4098,incubated 48 hours at 25–30° C.Escherichia coli ATCC 25922,incubated 24–48 hours at 35° C.

Negative control:Not applicable.


Quality Control and recommendedincubation conditions WL Differential:Positive control:Lactobacillus plantarum ATCC 8014,incubated 48 hours at 35° C.

Negative control:Sacchromyces cerivisiae ATCC 4098,incubated 48 hours at 25–30° C.


WL Nutrient broth is used for thedetermination of microbiological florain brewing and fermentation pro-cesses. WL differential media sup-presses the growth of yeast and moldand cultivated bacteria encounteredin brewing and industrial fermen-tation. The medium has the sameformulation as WL nutrient media buthas 4 mg of Cyclohexamide per literadded.

Description:Use of the medium at pH 5.5 andincubation at 25° C will give reliablecounts for brewer’s yeast. Adjustmentof the pH to 6.5 and incubation at 30° Callows for the selective growth ofbakers and distillers yeast.

Interpretation WL Nutrient Broth:Media will develop all yeast thatincubate at the selected temperatureand pH range.

WL Nutrient Broth

Wallerstein Nutrient Broth (WL) and WL Differential Broth (WLD)


Casein 5.0 gYeast Extract 4.0 gDextrose 50.0 gMonopotassium phospate 550 mgPotassium chloride 425 mgCalcium chloride 125 mgMagnesium sulfate 125 mgFerric chloride 2.5 mgManganese sulfate 2.5 mgBromocresol green 22 mg

Additional Component for WL Differential Broth:Cyclohexamide 4 mg

Culture of Sacchromyces cerivisiae ATCC 4098 onWallerstein Nutrient Broth (WL).

69


WL Nutrient Broth has a pH of 5.5 which is optimal for the enumeration ofbrewers’ yeast. In the case of cultivating microorganisms from an alcoholicmash, tomato juice should be added to the medium. WL Differential Mediacontains additionally 4 mg of Cyclohexamide per liter. This componentinhibits the developments of yeast without interfering with the developmentof bacteria generally encountered in beers. Cyclohexamide in WL Diffe-rential Broth is added to suppress yeast and any mold which may bepresent.

Wallerstein Nutrient Broth (WL) andWLDifferential Broth (WLD)


Escherichia coli ATCC 25922Growth

L. fermentum ATCC 9338 Growth



Escherichia coli ATCC 25922Growth

L. fermentum ATCC 9338 Growth

S. cerevisiae ATCC 9763 Partial to marked inhibition


Media Products

Wallerstein Nutrient Broth (WL) and WL Differential Broth (WLD)

Wallerstein Differential Broth (WLD): Cultur ofLactobacillus plantarum ATCC 8014.

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WL Nutrient Agar and WL Differential Agar were developed by GRAY andGREEN (1950), to study the microbial population of worts, beers, liquids,yeast, and other materials used in fermentation control procedures of thebrewing industry and other fermentation industries.The WL Nutrient Agar is used for the cultivation and enumeration of yeast.At pH 5.5 brewers’ yeast will grow unrestricted at 25º C within 24–48 hours.For growth of bakers’ and distillers’ yeast, the pH of the medium should beadjusted to 6.5 with incubation at 30º C for 2–7 days. Where yeast cells arein low number, certain bacteria can be detected.The WL Differential Broth is used to determine bacterial counts. GRAY andGREEN (1950) recommended using the differential medium to culturebacteria normally encountered in brewing; aerobic conditions for aceticacid and thermophilic bacteria, anaerobic conditions for lactic bacteria andbeer cocci.

Order information WL Nutrient Media

Ampoules 2 ml 50 10 496 108Petri dishes with sterile pads 47 mm 100 10 498 544Petri dishes with sterile pads 50 mm 50 10 445 905



Order information WL Differential Media

Ampoules 2 ml 50 10 496 109Bottled Media 50 ml 8 10 496 717*Petri dishes with sterile pads 47 mm 100 10 498 544Petri dishes with sterile pads 50 mm 50 10 445 905



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sus 6592 003 001.p1 ii.pdfeugon broth quality control and recommended incubation conditions:...

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