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Survey for Fishes in Freeman Creek System and North River, Fayette and Tuscaloosa Counties, Alabama, on 26 May 2012 Submitted to: Mike Watts 15439 Byler Trace Northport, AL By: Bernard R. Kuhajda, Ph.D. Department of Biological Sciences Box 870345 University of Alabama Tuscaloosa, Alabama 35487-0345 30 May 2012

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Page 1: Survey for Fishes in Freeman Creek System and North River ...media.al.com/spotnews/other/Freeman Creek Report on Fishes May … · Survey for Fishes in Freeman Creek System and North

Survey for Fishes in Freeman Creek System and North River, Fayette and Tuscaloosa Counties,

Alabama, on 26 May 2012

Submitted to:

Mike Watts

15439 Byler Trace

Northport, AL

By:

Bernard R. Kuhajda, Ph.D.

Department of Biological Sciences

Box 870345

University of Alabama

Tuscaloosa, Alabama 35487-0345

30 May 2012

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Survey for Fishes in Freeman Creek System and North River, Fayette and Tuscaloosa

Counties, Alabama, on 26 May 2012

Summary

Freeman Creek and its unnamed tributary west of US Hwy 43 in Fayette and Tuscaloosa

counties and the North River at and upstream of County Road 63 in Tuscaloosa County were

sampled for fishes on 26 May 2012 to assess the effects of a coal slurry spill into the unnamed

tributary on 15 July 2011 (Fig. 1). Based on all data collected over 11 months at these sites since

the slurry spill, aquatic habitat and the fish community has recovered or, in the case of fish

abundance in lower Freeman Creek, is on its way to recovery, therefore additional habitat and

fish surveys are not needed and biological monitoring can be terminated.

The coal slurry spill on 15 July 2011 negatively impacted the habitat, diversity, and

abundance of fishes and other aquatic taxa in the unnamed tributary and Freeman Creek in areas

downstream of the spill based on data collected 5 and 10 days after the event. This is especially

the case in the unnamed tributary, where habitat was buried by the slurry and practically no

fishes or other aquatic taxa were present. Evaluation of habitat and sampling of fishes and other

aquatic taxa on 26 May 2012 revealed that there was slightly less fish diversity present in the

impacted areas of the unnamed tributary (Sites 2 and 3) relative to the area upstream of the slurry

spill (Site 1) but diversity had increased over the last three months (March-May); the overall

trend since the coal slurry spill is upward. Abundance was higher compared to the previous

month at sites 1 and 2 and remained high at Site 3. The two previously impacted sites have had

an overall increasing trend since the coal slurry spill (Fig. 13). No coal slurry deposits were

observed in the unnamed tributary.

Diversity and of fishes was similar (14-16 species) at all three sites on Freeman Creek, with

an increasing trend at all sites since November 2011. Abundance of fishes has remained

relatively constant at the control site (Site 4) over the last six months but has shown downward

trends at both sites 5 and 6 over this same period, with abundance at Site 6 continuing to be very

low, likely associated with lasting effects of dewatering for slurry removal in late October (the

last habitat in Freeman Creek to be restored) (Fig. 14). Now that cleanup in Freeman Creek is

completed and all filter dams in lower Freeman Creek removed, restored habitat does allow

access for fishes to previously isolated areas and this should lead to further improvements in

abundance in the fish community in the coming months as fishes spawn in late spring and

summer.

The limited fish data collected in the North River in this study shows similar numbers from

August through April at County Road 63 (Site 8) and higher diversity and numbers in May

relative to the control site (Site 7).

Biotic recovery of the unnamed tributary is complete, with equal or higher abundance and

similar diversity of fishes compared to the unaffected stream reach. Freeman Creek has similar

diversity of fishes but lower abundance in impacted areas, especially the lower portion, however

biotic recovery should continue now that cleanup efforts are completed, habitat has recovered,

and all reaches in Freeman Creek are reconnected after the removal of all filter dams.

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Figure 1. Stream reaches surveyed for fishes in an unnamed tributary, Freeman Creek, and the

North River in Tuscaloosa and Fayette counties, Alabama, on 26 May 2012. See text for locality

and description of Sites 1 to 8.

Coal slurry spill site

1

2

1

4

5

6

7

8

3

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Figure 2. Unnamed tributary to Freeman Creek upstream of coal slurry spill (Site 1) on 26 May

2012 showing A) pool and B) riffle habitat.

A

B

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Figure 3. Unnamed tributary to Freeman Creek downstream of coal slurry spill (Site 2) on 26

May 2012 showing A) run, B) riffle, and C) pool habitat.

A

B

C

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Figure 4. Unnamed tributary to Freeman Creek downstream of coal slurry spill and Holcomb

Road (Site 3) on 26 May 2012 showing A) pool & B) riffle habitat.

A

B

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Figure 5. Freeman Creek upstream of coal slurry spill (Site 4) on 26 May 2012 showing A)

abandoned beaver dam just above unnamed tributary and B) no pool above beaver dam.

A

B

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Figure 6. Freeman Creek upstream of coal slurry spill (Site 4) on 26 May 2012 showing A) riffle

habitat below new beaver dam and B) bedrock riffle habitat at upper end of sampling area.

A

B

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Figure 7. Freeman Creek downstream of mouth of unnamed tributary, coal slurry spill input, and

US Hwy 43 (Site 5) on 26 May 2012 showing A) pool and B) riffle habitat.

A

B

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Figure 8. Lower Freeman Creek near mouth (Site 6) on 26 May 2012 showing A) riffle and B)

riffle/pool habitat downstream of former filter dams not inundated, low water in North River.

A

B

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Figure 9. Lower Freeman Creek (Site 6) on 26 May 2012 showing A) site of former new filter

dams and B) pool habitat upstream of former new filter dam site.

A

B

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Figure 10. Lower Freeman Creek (Site 6) on 26 May 2012 showing A) riffle habitat and B) site

of former upper (original) filter dams.

A

B

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Figure 11. North River at mouth of Freeman Creek (Site 7) on 26 May 2012 showing A) riffle

habitat and B) riffle and pool habitat.

A

B

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Figure 12. North River at Co. Rd. 63 (Site 8) on 26 May 2012 showing A) riffle and B) pool

habitat.

A

B

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14

0

2

4

6

8

10

12

14

16

18

Above spill Below spill Near mouth

July

August

September

October

November

December

January

February

March

April

May

0

5

10

15

20

25

30

35

40

Above spill Below spill Near mouth

July

August

September

October

November

December

January

February

March

April

May

Figure 13. A) Diversity (number of fish species per site) and B) abundance (average number of

specimens per seine haul) of fishes for unnamed tributary for July 2011–May 2012.

Unnamed Tributary Diversity

Nu

mb

er

of

spec

ies

per

sit

e

Ave

rage

nu

mb

er s

pec

imen

s p

er

sein

e h

aul

A

B

Site 1 Site 2 Site 3

Site 1 Site 2 Site 3

Unnamed Tributary Abundance

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15

0

5

10

15

20

25

Above spill Below spill Near mouth

July

August

September

October

November

December

January

February

March

April

May

0

5

10

15

20

25

30

35

40

45

50

Above spill Below spill Near mouth

July

August

September

October

November

December

January

February

March

April

May

Figure 14. A) Diversity (number of fish species per site) and B) abundance (average number of

specimens per seine haul) of fishes for Freeman Creek for July 2011–May 2012.

Freeman Creek Diversity

Freeman Creek Abundance

Nu

mb

er

of

spec

ies

per

sit

e

Ave

rage

nu

mb

er s

pec

imen

s p

er

sein

e h

aul

A

B

Site 4 Site 5 Site 6

Site 4 Site 5 Site 6

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Table 1. Fishes and other aquatic taxa collected in unnamed tributary above coal slurry spill, 26 May 2012.

Start 18:42, end 19:20 Number of Number of

Species R1 R2 R3 R4 P1 P2 P3 P4 RU1 R/RU1 R/RU2 R/P1 seines present specimens

Ammocoete 0 0

Ichthyomyzon gagei 0 0

Campostoma oligolepis 0 0

Cyprinella callistia 0 0

Luxilus chrysocephalus 2 3 2 1 1 5 9

Lythrurus bellus 1 1 1

Nocomis leptocephalus 1 4 1 6 4 1 8 7 25

Notropis baileyi 7 1 9 5 8 9 11 13 16 5 39 11 123

Notropis stilbius 0 0

Semotilus atromaculatus 2 2 2 1 4 7

Semotilus thoreauianus 2 2 2 2 3 2 2 7 15

Erimyzon oblongus 5 1 5

Moxostoma poecilurum 0 0

Noturus funebris 2 1 2

Noturus gyrinus 0 0

Noturus leptacanthus 0 0

Fundulus olivaceus 1 1 1

Lepomis cyanellus 1 1 2 2

Lepomis macrochirus 0 0

Lepomis megalotis 1 3 2 4

Micropterus punctulatus 0 0

Etheostoma artesiae 1 1 2 2

Etheostoma parvipinne 0 0

Etheostoma stigmaeum 0 0

Etheostoma swaini 2 1 2 3

Percina nigrofasciata 0 0

Number of species 1 2 2 2 6 5 8 4 3 4 1 8 Total 13

Number of specimens 7 3 2 11 16 15 31 18 16 20 5 55 Total 199

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Table 1 (continued).

Non-fish taxa

Dragonfly nymph 1 1 1 1 1 5 5

Damselfly nymph 0 0

Mayfly nymph 0 0

Stonefly nymph 2 1 2

Helgramites 0 0

Cranefly larva 0 0

Caddisfly larva 0 0

Tabanid larva 0 0

Crayfishes 9 7 1 1 1 3 1 7 23

Salamander 0 0

Number of taxa 1 2 2 1 0 1 1 1 2 1 0 1 Total 3

Number of specimens 9 8 3 1 0 1 1 1 4 1 0 1 Total 30

R1 R2 R3 R4 P1 P2 P3 P4 RU1 R/RU1 R/RU2 R/P1

R = Riffle

P = Pool

RU = Run

Start of sampling, P1 33o35'02"N, 87o40'10"W

End of sampling, R5 33o35'06"N, 87o40'15"W

Water lowest since fall sampling.

Beaver dam (33o35'02"N, 87o40'10"W) abandoned, riffle and pool complexes have returned.

P1 below beaver dam

Creek with lots of fine sediments, mostly sand.

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Table 2. Fishes and other aquatic taxa collected in unnamed tributary below coal slurry spill, 26 May 2012.

Start 17:52, end 18:37 Number of Number of

Species R1 R2 R3 R4 R5 P1 P2 P3 P4 P5 RU1 RU2 R/P1 seines present specimens

Campostoma oligolepis 0 0

Cyprinella callistia 0 0

Luxilus chrysocephalus 1 4 2 3 7

Lythrurus bellus 0 0

Nocomis leptocephalus 1 3 1 15 2 5 6 27

Notropis baileyi 16 4 11 9 26 3 1 1 2 43 10 116

Semotilus atromaculatus 1 2 3 4 1 5 3 20 5 9 44

Semotilus thoreauianus 3 4 8 1 4 2 4 3 2 3 1 11 35

Larval minnows 1 3 2 4

Erymyzon oblongus 0 0

Larval suckers 1 1 2 2

Moxostoma poecilurum 0 0

Ictalurus punctatus 0 0

Noturus funebris 0 0

Noturus gyrinus 0 0

Noturus leptacanthus 0 0

Pylodictis olivaris 0 0

Fundulus olivaceus 1 1 1

Lepomis cyanellus 0 0

Lepomis macrochirus 0 0

Lepomis megalotis 0 0

Micropterus punctulatus 0 0

Etheostoma artesiae 2 1 2 1 4 6

Etheostoma parvipinne 0 0

Etheostoma stigmaeum 0 0

Percina nigrofasciata 0 0

Number of species 2 2 4 4 2 5 7 6 3 4 2 2 5 Total 8

Number of specimens 4 6 27 8 8 18 21 53 8 26 3 5 55 Total 242

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Table 2 (continued).

Non-fish taxa

Dragonfly nymph 2 1 1 2 1 1 6 8

Mayfly nymph 1 1 2 2

Stonefly nymph 1 1 1

Damselfly nymph 0 0

Helgramites 0 0

Caddisfly larvae 0 0

Frogs 1 1 2 2

Crayfishes 3 1 2 3 6

Number of taxa 4 0 0 2 2 0 0 1 1 0 2 1 1 Total 5

Number of specimens 7 0 0 2 2 0 0 2 1 0 3 1 1 Total 19

R1 R2 R3 R4 R5 P1 P2 P3 P4 P5 RU1 RU2 R/P1

R = Riffle

P = Pool

RU = Run

Start of sampling, P1 33o34'57"N, 87

o39'58"W

End of sampling, R5 33o35'00"N, 87

o40'05"W

Water present and flowing throughout entire study reach including areas dry in August 2011.

Water lowest since fall sampling.

More sand present in stream since fall.

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Table 3. Fishes and other aquatic taxa collected in unnamed tributary below spill, just above its mouth, 26 May 2012.

Start 17:00, end 17:20 Number of Number of

Species R1 R2 P1 P2 P3 R/P1 seines present specimens

Campostoma oligolepis 0 0

Cyprinella callistia 0 0

Luxilus chrysocephalus 5 1 5 3 11

Lythrurus bellus 5 5 6 3 16

Nocomis leptocephalus 1 1 2 2

Notropis baileyi 3 1 13 4 32 3 6 56

Notropis stilbius 0 0

Semotilus atromaculatus 1 6 3 8 9 6 6 33

Semotilus thoreauianus 1 2 2 3

Erimyzon oblongus 0 0

Larval suckers 1 1 2 2

Ameiurus natalis 0 0

Ictalurus punctatus 0 0

Noturus funebris 0 0

Noturus gyrinus 0 0

Noturus leptacanthus 0 0

Pylodictis olivaris 0 0

Fundulus olivaceus 0 0

Lepomis cyanellus 0 0

Lepomis macrochirus 0 0

Lepomis megalotis 1 1 1

Micropterus punctulatus 0 0

Etheostoma artesiae 0 0

Etheostoma parvipinne 0 0

Etheostoma stigmaeum 1 1 1

Etheostoma swaini 0 0

Percina nigrofasciata 0 0

Number of species 4 3 5 5 5 4 Total 9

Number of specimens 6 8 28 19 53 11 Total 125

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Table 3 (continued).

Dragonfly nymph 0 0

Danselfly nymph 0 0

Mayfly nymph 0 0

Helgramites 0 0

Caddisfly larva 1 1 2 2

Beetle larvae 0 0

Cranefly larva 0 0

Crayfishes 4 2 1 3 7

Number of taxa 1 1 0 1 1 1 Total 5

Number of specimens 1 4 0 1 2 1 Total 9

R1 R2 P1 P2 P3 R/P1

R = Riffle

P = Pool

Start of sampling, R/P1 33o34'27"N, 87o39'34"W

End of sampling, P3 33o34'30"N, 87

o39'37"W

Slurry gone from pools and shoreline.

Water present and flowing throughout entire study reach including areas dry in August 2011.

Water lowest since fall sampling.

More sand present in stream since fall.

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Table 4. Fishes and other aquatic taxa collected in Freeman Creek above coal slurry spill and US Hwy 43, 26 May 2012.

Start 15:53, end 16:40 Number of Number of

Species R1 R2 R3 R4 R5 R6 P1 P2 P3 RU1 RUP1 INP1 seines present specimens

Ichthyomyzon gagei 0 0

Campostoma oligolepis 2 3 1 1 4 7

Cyprinella callistia 0 0

Luxilus chrysocephalus 1 13 2 3 16

Lythrurus bellus 1 20 3 1 4 25

Nocomis leptocephalus 4 2 3 2 2 5 13

Notropis baileyi 14 5 8 38 1 18 24 1 10 2 10 121

Semotilus atromaculatus 2 1 1 1 4 5

Semotilus thoreauianus 3 1 3

Erymyzon oblongus 0 0

Hypentelium etowanum 0 0

Moxostoma poecilurum 1 1 1

Ameiurus natalis 0 0

Ictalurus punctatus 0 0

Noturus funebris 0 0

Noturus leptacanthus 1 1 1

Fundulus olivaceus 1 1 1

Lepomis cyanellus 2 1 2

Lepomis marcrochirus 4 1 4

Lepomis megalotis 1 20 1 1 4 23

Larval darter 1 1 1

Etheostoma parvipinne 1 1 1

Etheostoma stigmaeum 4 6 3 1 3 1 2 6 8 26

Etheostoma swaini 0 0

Percina maculata 0 0

Percina nigrofasciata 3 1 1 2 4 7

Number of species 2 7 4 5 2 7 8 7 5 3 3 1 Total 16

Number of specimens 7 31 9 16 3 48 34 55 31 8 13 2 Total 257

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Table 4 (continued).

Non-fish taxa

Dragonfly nymph 4 4 5 1 5 5 19

Damselfly nymph 0 0

Mayfly nymph 0 0

Stonefly nymph 0 0

Helgramites 1 1 1

Tabanid larva 0 0

Cranefly larva 0 0

Caddisfly larva 3 1 3

Crayfishes 1 1 1 3 3

Tadpoles 0 0

Desmognathus salamander 0 0

Number of taxa 1 1 1 1 2 1 1 0 0 1 0 1 Total 4

Number of specimens 4 4 5 1 4 1 1 0 0 5 0 1 Total 26

R1 R2 R3 R4 R5 R6 P1 P2 P3 RU1 RUP1 INP1

R = Riffle

P = Pool

RU = Run

INP = Innundated Pool

Start of sampling, INP1 33o34'26"N, 87o39'36"W

Abandonded beaver dam 33o34'25"N, 87o39'38"W

End of sampling, above bedrock riffles, P1 33o34'23"N, 87

o39'47"W

Water lowest since fall sampling.

1st beaver dam at lower end of sampling reach abandoned, two pools now not inundated.

New beaver dam above Innundated Pool and below 1st abandoned beaver dam.

Beaver dam still abandonded halfway between 1st abandoned dam & bedrock riffle, inundated from new beaver dam.

Riffle 3 at base of 2nd beaver dam, Run/Pool at base of upstream bedrock riffle.

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Table 5. Fishes and other aquatic taxa collected in Freeman Creek below coal slurry spill, below US Hwy 43, 26 May 2012.

Start 14:42 end 15:25 Number of Number of

Species R1 R2 R3 R4 R5 P1 P2 P3 P4 P5 RU1 TOR1 seines present specimens

Campostoma oligolepis 1 1 3 3 5

Cyprinella callistia 0 0

Luxilus chrysocephalus 2 7 1 3 10

Lythrurus bellus 3 8 4 1 1 2 6 19

Nocomis leptocephalus 1 1 1 2 1 1 6 7

Notropis baileyi 4 1 1 1 6 10 9 3 10 3 10 48

Notropis stilbius 0 0

Semotilus atromaculatus 0 0

Semotilus thoreauianus 1 1 1

Larval minnows 1 1 1

Erimyzon oblongus 1 1 1

Moxostoma poecilurum 2 1 2 3

Ameiurus natalis 0 0

Noturus funebris 0 0

Noturus gyrinus 0 0

Noturus leptacanthus 0 0

Pylodictis olivaris 0 0

Fundulus olivaceus 1 2 2 3

Lepomis cyanellus 0 0

Lepomis macrochirus 0 0

Lepomis megalotis 5 3 2 1 2 5 13

Micropterus punctulatus 0 0

Etheostoma artesiae 1 1 1

Etheostoma parvipinne 0 0

Etheostoma stigmaeum 1 1 2 1 1 5 6

Percina kathae 4 1 1 3 6

Percina nigrofasciata 1 1 1 1 1 1 6 6

Number of species 2 2 3 5 5 8 8 3 9 5 3 2 Total 14

Number of specimens 5 2 3 5 11 28 32 7 13 7 13 4 Total 130

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Table 5 (continued).

Non-fish taxa

Dragonfly nymph 1 1 2 3 2 1 1 2 3 9 16

Damselfly nymph 0 0

Mayfly nymph 2 2 2 4

Stonefly nymph 1 1 2 2

Helgramites 4 1 2 5

Tabanid larva 0 0

Cranefly larva 0 0

Caddisfly larva 1 1 1 3 3

Tabanid larva 0 0

Crayfishes 2 1 1 2 1 5 7

Tadpoles 0 0

Number of taxa 4 2 4 0 3 1 0 2 1 0 2 4 Total 6

Number of specimens 8 3 6 0 5 2 0 2 1 0 4 6 Total 37

R1 R2 R3 R4 R5 P1 P2 P3 P4 P5 RU1 TOR1

R = Riffle

P = Pool

TOR = Top of riffle

Start of sampling, R1 33o34'26"N, 87

o39'21"W

Abandoned beaver dam 33o34'28"N, 87o39'24"W

End of sampling, P6 33o34'27"N, 87o39'29"W

Slurry gone from pools and shoreline.

Beaver dam still abandoned in upper reach of sample area, innundated former riffle now flowing.

Water level lowest since fall sampling.

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Table 6. Fishes and other aquatic taxa collected in Freeman Creek below spill, just above mouth to original filter dam, 26 May 2012.

Start 12:47, end 13:50 Number of Number of

Species R1 R2 R3 R4 R5 R6 R7 R8 R9 P1 P2 P3 P4 P5 P6 P7 P8 P9 TOR1 seines present specimens

Campostoma oligolepis 1 2 1 3 4

Cyprinella callistia 1 1 3 1 4 6

Cyprinella venusta 0 0

Hybopsis winchelli 0 0

Luxilus chrysocephalus 1 1 2 2

Lythrurus bellus 2 2 1 1 4 6

Nocomis leptocephalus 0 0

Notropis baileyi 1 1 3 2 4 7

Notropis stilbius 0 0

Semotilus atromaculatus 1 4 1 3 6

Semotilus thoreauianus 3 2 1 3 6

Cyprinid larvae 0 0

Erimyzon oblongus 0 0

Larval suckers 1 1 1 2 4 5

Moxostoma poecilurum 0 0

Noturus funebris 0 0

Noturus leptacanthus 1 1 1

Fundulus olivaceus 0 0

Gambusia affinis 0 0

Lepomis cyanellus 0 0

Lepomis macrochirus 1 1 2 2

Lepomis megalotis 1 1 2 3 4

Micropterus salmoides 1 1 2 2

Etheostoma artesiae 0 0

Etheostoma stigmaeum 1 3 1 3 3 1 3 4 3 4 10 26

Percina maculata 1 1 1

Percina nigrofasciata 1 3 1 2 1 5 8

Larval darters 2 1 2

Number of species 4 2 6 2 3 5 3 3 1 6 1 3 1 2 2 3 2 1 2 Total 15

Number of specimens 4 6 6 4 9 9 7 7 1 9 1 4 1 4 2 4 3 1 6 Total 88

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Table 6 (continued).

Non-fish taxa

Dragonfly nymph 1 1 2 1 1 1 2 1 3 9 13

Damselfly nymph 1 1 1

Mayfly nymph 1 1 1

Stonefly nymph 1 1 1 3 3

Helgramites 0 0

Caddisfly larva 0 0

Cranefly larva 0 0

Backswimmer 0 0

Crayfishes 1 1 1 2 1 3 1 4 8 14

Tadpoles 1 1 1

Number of taxa 2 0 0 1 1 3 4 1 2 1 0 2 0 2 0 1 1 0 2 Total 6

Number of specimens 2 0 0 1 1 3 5 2 2 1 0 4 0 2 0 2 1 0 7 Total 33

R1 R2 R3 R4 R5 R6 R7 R8 R9 P1 P2 P3 P4 P5 P6 P7 P8 P9 TOR1

R = Riffle

P = Pool

TOR = Top of Riffle

R1 33o33'47"N, 87o38'16"W

End of sampling below former original filter dam 33o33'55"N, 87o38'28"W

1st 2 Riffles, 1 Top of Riffle, and pool below former filter dams NOT innundated from high water.

Water lowest since fall sampling.

Substrate above former lower filter dams dominated by bedrock (no pools) up to small tributary from the north.

No coal slurry depostits observed.

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Table 7. Fishes & other aquatic taxa collected in North River above spill, above Freeman Crk, 26 May 2012.

Start 12:30, end 12:42 Number of Number of

Species R1 R2 R3 P1 P2 TOR1 seines present specimens

Campostoma oligolepis 0 0

Cyprinella callistia 5 1 5

Luxilus chrysocephalus 0 0

Lythrurus bellus 0 0

Nocomis leptocephalus 0 0

Notropis stilbius 1 1 1

Notropis texanus 0 0

Hypentelium etowanum 0 0

Moxostoma poecilurum 0 0

Fundulus olivaceus 0 0

Gambusia affinis 0 0

Lepomis megalotis 0 0

Micropterus coosae 0 0

Micropterus punctulatus 0 0

Etheostoma stigmaeum 6 12 1 3 19

Percina kathae 0 0

Percina maculata 0 0

Percina nigrofasciata 1 1 2 2

Percina sciera 0 0

Number of species 0 4 2 0 0 1 Total 4

Number of specimens 0 13 13 0 0 1 Total 27

Non-fish taxa

Dragonfly nymph 2 1 2

Mayfly nymph 1 1 1

Stonefly nymph 2 1 2

Caddisfly larvae 0 0

Helgramites 0 0

Crayfishes 1 1 2 2

Tadpoles 0 0

Eurycea salamander 0 0

Number of species 0 3 1 1 0 0 Total 4

Number of specimens 0 4 2 1 0 0 Total 7

Species R1 R2 R3 P1 P2 TOR1

8 Elimia carinifera collected.

3 Corbicula collected.

R = Riffle

P = Pool

TOR = Top of Riffle

Confluence w/ Freeman Ck 33o33'46"N, 87

o38'16"w

Upstream of Freeman Ck 33o33'47"N, 87o38'15"w

Water lowest since fall sampling.

Distinct habitats lacking due to changes in river morphology at this site.

No coal slurry present, including shoreline and backwater areas.

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Table 8. Fishes and other aquatic taxa collected in North River below spill, Co. Rd. 63, 26 May 2012.

Start 11:18, end 11:45 Number of Number of

Species R1 R2 R3 P1 TOR1 TOR2 seines present specimens

Lepisosteus sp. juvenile 1 1 1

Campostoma oligolepis 9 2 6 1 4 18

Cyprinella callistia 2 4 5 7 4 18

Cyprinella venusta 0 0

Luxilus chrysocephalus 0 0

Lythrurus bellus 0 0

Nocomis leptocephalus 1 1 1

Notropis stilbius 5 12 1 3 5 5 26

Notropis texanus 0 0

Semotilus atromaculatus 2 1 2

Larval minnows 50 1 50

Larval suckers 4 2 10 3 16

Fundulus olivaceus 0 0

Gambusia affinis 0 0

Lepomis macrochirus 0 0

Lepomis megalotis 0 0

Micropterus coosae 0 0

Micropterus punctulatus 1 1 1

Etheostoma stigmaeum 1 11 2 1 4 15

Percina kathae 1 1 1

Percina maculata 2 1 1 3 4

Percina nigrofasciata 5 4 1 1 4 11

Larval darters 1 1 1

Number of species 7 4 7 8 4 4 Total 12

Number of specimens 28 22 14 82 5 14 Total 165

Non-fish taxa

Dragonfly nymph 2 1 2

Damselfly nymph 1 1 2 2

Stonefly nymph 0 0

Cranefly larvae 0 0

Helgramites 1 1 1

Crayfishes 1 5 2 6

Tadpoles 1 1 1

Eurycea salamander 0 0

Number of species 4 0 1 1 1 0 Total 5

Number of specimens 5 0 1 1 5 0 Total 12

Species R1 R2 R3 P1 TOR1 TOR2

150+ Elimia carinifera snails collected.

9 Corbicula clams collected.

R = Riffle

P = Pool

TOR = Top of Riffle

Sampling near bridge 33o33'43"N, 87o37'49"W

No coal slurry on bedrock in shallower water or in Justicia beds in backwaters.

Water lowest since fall sampling.

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Table 9. Scientific names, common names, and families of fishes collected in the Freeman Creek

watershed and the North River during this study.

Family Ichthyomyzon – Lampreys

Ichthyomyzon gagei Southern Brook Lamprey

Family Cyprinidae – Minnows, shiners, and chubs

Campostoma oligolepis Largescale Stoneroller

Cyprinella callistia Alabama Shiner

Cyprinella venusta Blacktail Shiner

Hybopsis winchelli Clear Chub

Luxilus chrysocephalus Striped Shiner

Lythrurus bellus Pretty Shiner

Nocomis leptocephalus Bluehead Chub

Notropis baileyi Rough Shiner

Notropis stilbius Silverstripe Shiner

Notropis texanus Weed Shiner

Semotilus atromaculatus Creek Chub

Semotilus thoreauianus Dixie Chub

Family Catostomidae – Suckers and redhorses

Erimyzon oblongus Creek Chubsucker

Hypentelium etowanum Alabama Hog Sucker

Minytrema melanops Spotted Sucker

Moxostoma poecilurum Blacktail Redhorse

Family Ictaluridae – Catfishes, bullheads, and madtoms

Amieurus natalis Yellow Bullhead

Ictalurus punctatus Channel Catfish

Noturus funebris Speckled Madtom

Noturus gyrinus Tadpole Madtom

Noturus leptacanthus Speckled Madtom

Pylodictis olivaris Flathead Catfish

Family Fundulidae - Topminnows

Fundulus olivaceus Blackspotted Topminnow

Family Poeciliidae – Mosquitofishes

Gambusia affinis Western Mosquitofish

Family Centrarchidae – Sunfishes and basses

Lepomis cyanellus Green Sunfish

Lepomis macrochirus Bluegill

Lepomis megalotis Longear Sunfish

Micropterus coosae Redeye Bass

Micropterus punctulatus Spotted Bass

Family Percidae - Darters

Etheostoma artesiae Redspot Darter

Etheostoma parvipinne Goldstripe Darter

Etheostoma stigmaeum Speckled Darter

Etheostoma swaini Gulf Darter

Percina kathae Mobile Logperch

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Percina maculata Blackside Darter

Percina nigrofasciata Blackbanded Darter

Percina sciera Dusky Darter