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SUPPLEMENTARY MATERIAL

for the doc.funds application

“Molecular Physiology in Aging, Metabolism and Lipids”

INDEX

CV – Univ.-Prof. Dr. Ronald Kühnlein 2

CV – Univ.-Prof. Dr. Frank Madeo 6

CV – Assoz. Prof. Dr. Monika Oberer 21

CV – Assoz. Prof. Dr. Stefan Schild 26

CV – Univ.-Prof. Dr. Ulrich Stelzl 31

CV – Ao. Univ.-Prof. Dr. Klaus Zangger 36

CV – Ao. Univ.-Prof. Dr. Ellen L. Zechner 44

CV – Univ.-Prof. Dr. Rudolf Zechner 49

CV – Assoz. Prof. Dr. Robert Zimmermann 63

Patents of the consortium members 69

Research grants of the consortium members (since 2011) 70

Prizes and awards of the consortium members (since 2011) 74

CV - Kühnlein

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Curriculum Vitae – Univ.- Prof. Dr. rer. nat. Ronald P. Kühnlein Institute of Molecular Biosciences, University of Graz, Humboldtstrasse 50, 2nd floor, 8010 Graz, Austria, Phone: +43 (0) 316 380 5504, E-Mail: [email protected] Year/ Place of Birth *1966/ Berlin, Germany Nationality German Orcid ID http://orcid.org/ 0000-0003-1448-4117 No of Publications 33 (22 first and/or corresponding) h-Index 20 (Scopus, Nov, 2016) Supervised Ph.D. Students since 2010: 2 finished, 1 ongoing Key words Drosophila melanogaster, lipid metabolism, energy homeostasis,

lipases, lipid droplet proteins, inter-organ communication, hormonal signaling, obesity

Professional experiences

since Oct. 2016 University Professor for Biochemistry, Institute of Molecular Biosciences, University of Graz, Austria

2010-2016 Independent Research Group head “Molecular Physiology”, Max Planck Institute for biophysical Chemistry, Göttingen, Germany

2000-2009 Project group head, Department Molecular Developmental Biology, Max Planck Institute for biophysical Chemistry, Göttingen, Germany

1998-1999 Research scientist, Max Planck Institute for biophysical Chemistry, Göttingen, Germany

1998 Visiting scientist at the Rockefeller University, New York, USA 1997-1998 Postdoctoral fellow, Max Planck Institute for biophysical Chemistry,

Göttingen, Germany 1996-1997 Postdoctoral fellow, Georg-August-University, Göttingen, Germany Education 2012 Habilitation in “Developmental Biology”, Georg-August-University,

Göttingen, Germany 1996 Dr. rer. nat., Eberhard-Karls-University, Tübingen, Germany

(“summa cum laude”) 1991 Diploma in Biology (“with distinction”; Subjects: Genetics, Microbiology,

Biochemistry), Ludwig-Maximilians-University, Munich, Germany Honors ▪ Awards 1985-1990 Undergraduate fellow of the “Studienstiftung des deutschen Volkes” Career related activities 2010-2016 GVO safety officer, Department of Molecular Developmental Biology,

Max Planck Institute for biophysical Chemistry, Göttingen, Germany 1998-2000 Scientific consultant, DeveloGen AG, Göttingen, Germany since 2000 Scientific reviewer for 34 Journals (e.g. Nature, Nat. Methods, Cell

Metabolism, Trends in Cell Biology, Nat. Commun., EMBO Journal, PNAS, Trends in Endocrinology and Metabolism, PLoS Genetics (also guest editor), Cell Reports), research foundations FWF (AT), DFG (DE), Welcome Trust (GB), NWO (NL), GACR (CZ), HFSP (FR), Boehringer Ingelheim Fund (DE), DAAD (DE) and universitites

for Universities: Univ. of Rochester (USA), Washington Univ. Conference organization: 18th European Drosophila Research Conference, Göttingen, Germany 21st European Drosophila Research Conference, Nice, France

2008 Selection panel member “Studienstiftung des Deutschen Volkes”

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Externally funded national and international projects (since 2011)

Funding Org.

Project Number

Research Topic Amount Funded [kEuro]

Funding Period

Max Planck Society

Molecular Physiology of Drosophila 700 2010-2016

DFG KU 2866/1-1 Regulation of Drosophila

melanogaster body fat content by store-operated calcium entry

133 2013-2016

Behrens Weise

Foundation

Nutrigenomics of Obesity using the Drosophila Model Organism 150 2013-2015

Publications [1] Klepsatel, P., Gáliková, M., Xu, Y., Kühnlein, R.P., Thermal stress depletes energy

reserves in Drosophila. Sci. Rep., The Author(s) SN -: 2016; Vol. 6, pp 33667 EP --33612. [2] Gáliková, M., Klepsatel, P., Xu, Y., Kühnlein, R.P., The obesity-related Adipokinetic

hormone controls feeding and expression of neuropeptide regulators of Drosophilametabolism. Eur. J. Lipid Sci. Technol., 2016; pp 1-14.

[3] Gáliková, M., Diesner, M., Klepsatel, P., Hehlert, P., Xu, Y., Bickmeyer, I., Predel, R., Kühnlein, R.P., Energy Homeostasis Control in Drosophila Adipokinetic Hormone Mutants. Genetics, Genetics Society of America: 2015; Vol. 201, pp 665-683.

[4] Baumbach, J., Xu, Y., Hehlert, P., Kühnlein, R.P., Galphaq, Ggamma1 and Plc21C Control Drosophila Body Fat Storage. J Genet Genomics, Elsevier Limited and Science Press: 2014; Vol. 41, pp 283-292.

[5] Baumbach, J., Hummel, P., Bickmeyer, I., Kowalczyk, K.M., Frank, M., Knorr, K., Hildebrandt, A., Riedel, D., Jäckle, H., Kühnlein, R.P., A Drosophila In Vivo Screen Identifies Store-Operated Calcium Entry as a Key Regulator of Adiposity. Cell Metab, Elsevier Inc.: 2014; Vol. 19, pp 331-343.

[6] Li, Z., Thiel, K., Thul, P.J., Beller, M., Kühnlein, R.P., Welte, M.A., Lipid droplets control the maternal histone supply of Drosophila embryos. Curr Biol, 2012; Vol. 22, pp 2104-2113.

[7] Kühnlein, R.P., Thematic review series: Lipid droplet synthesis and metabolism: from yeast to man. Lipid droplet-based storage fat metabolism in Drosophila. The Journal of Lipid Research, 2012; Vol. 53, pp 1430-1436.

[8] Birner-Gruenberger, R., Bickmeyer, I., Lange, J., Hehlert, P., Hermetter, A., Kollroser, M., Rechberger, G.N., Kühnlein, R.P., Functional fat body proteomics and gene targeting reveal in vivo functions of Drosophila melanogaster alpha-Esterase-7. Insect Biochemistry and Molecular Biology, Elsevier Ltd: 2012; Vol. 42, pp 220-229.

[9] Bi, J., Xiang, Y., Chen, H., Liu, Z., Grönke, S., Kühnlein, R.P., Huang, X., Opposite and redundant roles of the two Drosophila perilipins in lipid mobilization. J Cell Sci, The Company of Biologists Ltd: 2012; Vol. 125, pp 3568-3577.

[10] Kühnlein, R.P., The contribution of the Drosophila model to lipid droplet research. Progress in Lipid Research, Elsevier Ltd: 2011; Vol. 50, pp 348-356.

[11] Hildebrandt, A., Bickmeyer, I., Kühnlein, R.P., Reliable Drosophila Body Fat Quantification by a Coupled Colorimetric Assay. PLoS ONE, 2011; Vol. 6, p e23796.

[12] Kühnlein, R.P., Drosophila as a lipotoxicity model organism--more than a promise? Biochim Biophys Acta, 2010; Vol. 1801, pp 215-221.

[13] Kühnlein, R.P., Energy homeostasis regulation in Drosophila: a lipocentric perspective. Results Probl Cell Differ, 2010; Vol. 52, pp 159-173.

[14] Beller, M., Bulankina, A.V., Hsiao, H.-H., Urlaub, H., Jäckle, H., Kühnlein, R.P., PERILIPIN-dependent control of lipid droplet structure and fat storage in Drosophila. Cell Metab, 2010; Vol. 12, pp 521-532.

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[15] Kühnlein, R.P., Putting the brakes on dietary fat breakdown. Cell Metab, 2009; Vol. 10, pp 438-439.

[16] Grönke, S., Bickmeyer, I., Wunderlich, R., Jäckle, H., Kühnlein, R.P., Curled encodes the Drosophila homolog of the vertebrate circadian deadenylase Nocturnin. Genetics, 2009; Vol. 183, pp 219-232.

[17] Grönke, S., Müller, G., Hirsch, J., Fellert, S., Andreou, A., Haase, T., Jäckle, H., Kühnlein, R.P., Dual lipolytic control of body fat storage and mobilization in Drosophila. PLoS Biol, 2007; Vol. 5, p e137.

[18] Döring, F., Scholz, H., Kühnlein, R.P., Karschin, A., Wischmeyer, E., Novel Drosophila two-pore domain K channels: rescue of channel function by heteromeric assembly. Eur J Neurosci, Blackwell Publishing Ltd: 2006; Vol. 24, pp 2264-2274.

[19] Beller, M., Riedel, D., Jänsch, L., Dieterich, G., Wehland, J., Jäckle, H., Kühnlein, R.P., Characterization of the Drosophila lipid droplet subproteome. In Mol Cell Proteomics, 2006; Vol. 5, pp 1082-1094.

[20] Grönke, S., Mildner, A., Fellert, S., Tennagels, N., Petry, S., Müller, G., Jäckle, H., Kühnlein, R.P., Brummer lipase is an evolutionary conserved fat storage regulator in Drosophila. Cell Metab, 2005; Vol. 1, pp 323-330.

[21] Häder, T., Müller, S., Aguilera, M., Eulenberg, K.G., Steuernagel, A., Ciossek, T., Kühnlein, R.P., Lemaire, L., Fritsch, R., Dohrmann, C., Vetter, I.R., Jäckle, H., Doane, W.W., Brönner, G., Control of triglyceride storage by a WD40/TPR-domain protein. EMBO Rep, 2003; Vol. 4, pp 511-516.

[22] Grönke, S., Beller, M., Fellert, S., Ramakrishnan, H., Jäckle, H., Kühnlein, R.P., Control of fat storage by a Drosophila PAT domain protein. Current Biology, 2003; Vol. 13, pp 603-606.

[23] Döring, F., Wischmeyer, E., Kühnlein, R.P., Jäckle, H., Karschin, A., Inwardly rectifying K+ (Kir) channels in Drosophila. A crucial role of cellular milieu factors Kir channel function. JBC, American Society for Biochemistry and Molecular Biology: 2002; Vol. 277, pp 25554-25561.

[24] Mollereau, B., Wernet, M.F., Beaufils, P., Killian, D., Pichaud, F., Kühnlein, R., Desplan, C., A green fluorescent protein enhancer trap screen in Drosophila photoreceptor cells. Mech Dev, 2000; Vol. 93, pp 151-160.

[25] Loosli, F., Köster, R.W., Carl, M., Kühnlein, R., Henrich, T., Mücke, M., Krone, A., Wittbrodt, J., A genetic screen for mutations affecting embryonic development in medaka fish (Oryzias latipes). Mech Dev, 2000; Vol. 97, pp 133-139.

[26] Köster, R.W., Kühnlein, R.P., Wittbrodt, J., Ectopic Sox3 activity elicits sensory placode formation. Mech Dev, 2000; Vol. 95, pp 175-187.

[27] Kühnlein, R.P., Chen, C.K., Schuh, R., A transcription unit at the ken and barbie gene locus encodes a novel Drosophila zinc finger protein. Mech Dev, 1998; Vol. 79, pp 161-164.

[28] Chen, C.K., Kühnlein, R.P., Eulenberg, K.G., Vincent, S., Affolter, M., Schuh, R., The transcription factors KNIRPS and KNIRPS RELATED control cell migration and branch morphogenesis during Drosophila tracheal development. Development, 1998; Vol. 125, pp 4959-4968.

[29] Kühnlein, R.P., Bronner, G., Taubert, H., Schuh, R., Regulation of Drosophila spalt gene expression. Mech Dev, 1997; Vol. 66, pp 107-118.

[30] Reuter, D., Kühnlein, R.P., Frommer, G., Barrio, R., Kafatos, F.C., Jackle, H., Schuh, R., Regulation, function and potential origin of the Drosophila gene spalt adjacent, which encodes a secreted protein expressed in the early embryo. Chromosoma, 1996; Vol. 104, pp 445-454.

[31] Kühnlein, R.P., Schuh, R., Dual function of the region-specific homeotic gene spalt during Drosophila tracheal system development. Development, 1996; Vol. 122, pp 2215-2223.

[32] Kohlhase, J., Schuh, R., Dowe, G., Kühnlein, R.P., Jackle, H., Schroeder, B., Schulz-Schaeffer, W., Kretzschmar, H.A., Köhler, A., Muller, U., Raab-Vetter, M., Burkhardt, E., Engel, W., Stick, R., Isolation, characterization, and organ-specific expression of two novel human zinc finger genes related to the Drosophila gene spalt. Genomics, 1996; Vol. 38, pp 291-298.

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[33] Kühnlein, R.P., Frommer, G., Friedrich, M., Gonzalez-Gaitan, M., Weber, A., Wagner-Bernholz, J.F., Gehring, W.J., Jackle, H., Schuh, R., spalt encodes an evolutionarily conserved zinc finger protein of novel structure which provides homeotic gene function in the head and tail region of the Drosophila embryo. EMBO J, 1994; Vol. 13, pp 168-179.

Selected oral presentations 2016 Ludwig-Maximilians-University, Munich, Germany

2016 FASEB conference, “Lipid droplets: Dynamic Organelles in Metabolism and Beyond”

Snowball, CO, USA

2016 Annual Invertebrate Neuropeptide Conference, Ouro Preto, Brazil 2015 DGF Annual Meeting, Heidelberg, Germany 2015 Annual Invertebrate Neuropeptide Conference, Bagan, Myanmar 2015 9th International Congress of Comparative Physiology and Biochemistry, Krakow,

Poland 2015 Workshop “Molecular Methods in Insect Physiology”, České Budějovice, Czech

Republic 2014 Annual Invertebrate Neuropeptide Conference, El Calafate, Argentina

2014 5th Intl. Graz Symposium on Lipid and Membrane Biology, Graz, Austria

2014 University of Kassel, Germany

2012 60th Annual Meeting of the Entomological Society of America, Knoxville, USA

2011 1st German Fly Metabolism Meeting, Cologne, Germany

2011 17th International Biophysics Congress satellite meeting: Lipid metabolism and

function“, Beijing, People`s Republic of China

2011 Technical University of Munich, Germany

2011 Keystone Symposium „Lipid Biology and Lipotoxicity“, Killarney, Ireland

CV - Madeo

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Curriculum Vitae – Univ.- Prof. Dr. rer. nat. Frank Madeo Institute of Molecular Biosciences, University of Graz, Humboldtstrasse 50, 1st floor 8010 Graz, Austria, Phone: +43 (0) 316 380 8878, E-Mail: [email protected] Year/ Place of Birth *1967/ Schwerte, Germany Nationality Germany Orcid ID orcid.org/0000-0002-5070-1329 No of Publications 172 (74 first and/or corresponding) h-Index 57 (Scopus, Nov, 2016) Supervised Ph.D. Students since 2007: 14 finished, 6 ongoing Key words Apoptosis, Autophagy and Aging in yeast, flies and mammalian model

systems

Professional experiences since 2004 Contract as a Full Professor at the University of Graz, Austria 1997-2004 Group Leader at the University of Tübingen, Germany Education 1994-1997 Ph.D. thesis “Tyrosin phosphorylation and apoptosis in yeast”, University

of Tübingen, Germany, with first class honors 1987-1993 Studies of Biochemistry, University of Tübingen, Germany Honors ▪ Awards 2015 Erzherzog-Johann Research Award 2003-2004 Heisenberg fellow of the Deutsche Forschungsgemeinschaft Career related activities 2002 Guest Professor and teaching activity in the Master Course on Molecular

Genetics, Minho, University of Braga, Portugal 2002 Organization of the 1st International Meeting on Yeast Apoptosis (IMYA),

Minho, University of Braga, Portugal 2005 Organization of the 4th IMYA, Miami, FL, USA since 2007 Vice Speaker of the FWF-funded research consortium SFB LIPOTOX 2008 Guest Editor for BBA Molecular Cell Research coordinating a special

issue “Apoptosis in Yeast” 2009 Organization of the 7th IMYA, Graz, Austria 2009 Best publishing scientist of the University of Graz 2009/2010 Guest Editor for Cell Death and Differentiation coordinating a special

issue “Apoptosis in Yeast” 2013 Guest Editor for BBA Molecular Cell Research coordinating a special

issue “Cell Death Pathways” 2014 Editor in Chief Microbial Cell

mailto:[email protected]

CV - Madeo

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Funding Org.

Project Number


Funding Period

FWF P24381 Causes of spermidine-mediated

longevity 395 2012-2015

FWF/DFG “DACH”

I1000 Linking mitochondrial dynamics with

neurotoxicity in yeast cell death models

144 2012-2015

FWF P23490 Autophagic cell death:

characteristics and regulation 376 2011-2014

FWF F30

SFB LIPOTOX – Epigenetics of Lipid Overload during Aging: The

Crutial Role of Acetyl-CoA 1st, 2nd and 3rd Funding Period

1110 2007-2017

FWF W1226 DK Metabolic and Cardiovascular

Disease 392 2010-2018

University support

- Unconventional research programm 420 2014-2018

Austrian Ministry of Science

- FlySleep 184 2014-2017

FWF P 29203 Causal epigenetic changes during

aging and caloric restriction 352 2016-2019

FWF P 29262 Mechanisms of Spermidine mediated neuroprotection

339 2016-2019

FWF P 27893 Pro-autophagic Polyphenols and

Polyamines for Longevity 453 2015-2018

Publications [1] Carmona-Gutierrez D, Hughes AL, Madeo F, and Ruckenstuhl C 2016. The crucial

impact of lysosomes in aging and longevity. Ageing Res Rev 32: 2–12. doi: 10.1016/j.arr.2016.04.009.

[2] Eisenberg T, Abdellatif M, Schroeder S, Primessnig U, Stekovic S, Pendl T, Harger A, Schipke J, Zimmermann A, Schmidt A, Tong M, Ruckenstuhl C, Dammbrueck C, Gross AS, Herbst V, Magnes C, Trausinger G, Narath S, Meinitzer A, Hu Z, Kirsch A, Eller K, Carmona-Gutierrez D, Büttner S, Pietrocola F, Knittelfelder O, Schrepfer E, Rockenfeller P, Simonini C, Rahn A, Horsch M, Moreth K, Beckers J, Fuchs H, Gailus-Durner V, Neff F, Janik D, Rathkolb B, Rozman J, de Angelis MH, Moustafa T, Haemmerle G, Mayr M, Willeit P, von Frieling-Salewsky M, Pieske B, Scorrano L, Pieber T, Pechlaner R, Willeit J, Sigrist SJ, Linke WA, Mühlfeld C, Sadoshima J, Dengjel J, Kiechl S, Kroemer G, Sedej S, and Madeo F 2016. Cardioprotection and lifespan extension by the natural polyamine spermidine. Nat Med doi: 10.1038/nm.4222.

[3] Macheiner T, Fengler VHI, Agreiter M, Eisenberg T, Madeo F, Kolb D, Huppertz B, Ackbar R, and Sargsyan K 2016. Magnetomitotransfer: An efficient way for direct mitochondria transfer into cultured human cells. Sci Rep 6: 35571. doi: 10.1038/srep35571.

[4] Pietrocola F, Pol J, Vacchelli E, Baracco EE, Levesque S, Castoldi F, Maiuri MC, Madeo F, and Kroemer G 2016. Autophagy induction for the treatment of cancer. Autophagy 12(10): 1962–1964. doi: 10.1080/15548627.2016.1214778.

[5] Gupta VK, Pech U, Bhukel A, Fulterer A, Ender A, Mauermann SF, Andlauer TFM, Antwi-Adjei E, Beuschel C, Thriene K, Maglione M, Quentin C, Bushow R, Schwärzel M, Mielke

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T, Madeo F, Dengjel J, Fiala A, and Sigrist SJ 2016. Spermidine Suppresses Age-Associated Memory Impairment by Preventing Adverse Increase of Presynaptic Active Zone Size and Release. PLoS Biol 14(9): e1002563. doi: 10.1371/journal.pbio.1002563.

[6] López-Otín C, Galluzzi L, Freije JMP, Madeo F, and Kroemer G 2016. Metabolic Control of Longevity. Cell 166(4): 802–821. doi: 10.1016/j.cell.2016.07.031.

[7] Pietrocola F, Pol J, Vacchelli E, Rao S, Enot DP, Baracco EE, Levesque S, Castoldi F, Jacquelot N, Yamazaki T, Senovilla L, Marino G, Aranda F, Durand S, Sica V, Chery A, Lachkar S, Sigl V, Bloy N, Buque A, Falzoni S, Ryffel B, Apetoh L, Di Virgilio F, Madeo F, Maiuri MC, Zitvogel L, Levine B, Penninger JM, and Kroemer G 2016. Caloric Restriction Mimetics Enhance Anticancer Immunosurveillance. Cancer Cell 30(1): 147–160. doi: 10.1016/j.ccell.2016.05.016.

[8] Mitchell SJ, Madrigal-Matute J, Scheibye-Knudsen M, Fang E, Aon M, González-Reyes JA, Cortassa S, Kaushik S, Gonzalez-Freire M, Patel B, Wahl D, Ali A, Calvo-Rubio M, Burón MI, Guiterrez V, Ward TM, Palacios HH, Cai H, Frederick DW, Hine C, Broeskamp F, Habering L, Dawson J, Beasley TM, Wan J, Ikeno Y, Hubbard G, Becker KG, Zhang Y, Bohr VA, Longo DL, Navas P, Ferrucci L, Sinclair DA, Cohen P, Egan JM, Mitchell JR, Baur JA, Allison DB, Anson RM, Villalba JM, Madeo F, Cuervo AM, Pearson KJ, Ingram DK, Bernier M, and de Cabo R 2016. Effects of Sex, Strain, and Energy Intake on Hallmarks of Aging in Mice. Cell Metab 23(6): 1093–1112. doi: 10.1016/j.cmet.2016.05.027.

[9] Rockenfeller P, Carmona-Gutierrez D, Pietrocola F, Kroemer G, and Madeo F 2016. Ethanolamine: A novel anti-aging agent. Mol Cell Oncol 3(1): e1019023. doi: 10.1080/23723556.2015.1019023.

[10] Klionsky DJ, Abdelmohsen K, Abe A, Abedin MJ, Abeliovich H, Acevedo Arozena A, Adachi H, Adams CM, Adams PD, Adeli K, Adhihetty PJ, Adler SG, Agam G, Agarwal R, Aghi MK, Agnello M, Agostinis P, Aguilar PV, Aguirre-Ghiso J, Airoldi EM, Ait-Si-Ali S, Akematsu T, Akporiaye ET, Al-Rubeai M, Albaiceta GM, Albanese C, Albani D, Albert ML, Aldudo J, Algül H, Alirezaei M, Alloza I, Almasan A, Almonte-Beceril M, Alnemri ES, Alonso C, Altan-Bonnet N, Altieri DC, Alvarez S, Alvarez-Erviti L, Alves S, Amadoro G, Amano A, Amantini C, Ambrosio S, Amelio I, Amer AO, Amessou M, Amon A, An Z, Anania FA, Andersen SU, Andley UP, Andreadi CK, Andrieu-Abadie N, Anel A, Ann DK, Anoopkumar-Dukie S, Antonioli M, Aoki H, Apostolova N, Aquila S, Aquilano K, Araki K, Arama E, Aranda A, Araya J, Arcaro A, Arias E, Arimoto H, Ariosa AR, Armstrong JL, Arnould T, Arsov I, Asanuma K, ….Madeo F, … Zhou S-F, Zhou X-J, Zhu H, Zhu H, Zhu W-G, Zhu W, Zhu X-F, Zhu Y, Zhuang S-M, Zhuang X, Ziparo E, Zois CE, Zoladek T, Zong W-X, Zorzano A, and Zughaier SM 2016. Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy 12(1): 1–222. doi: 10.1080/15548627.2015.1100356.

[11] Enot DP, Niso-Santano M, Durand S, Chery A, Pietrocola F, Vacchelli E, Madeo F, Galluzzi L, and Kroemer G 2015. Metabolomic analyses reveal that anti-aging metabolites are depleted by palmitate but increased by oleate in vivo. Cell Cycle 14(15): 2399–2407. doi: 10.1080/15384101.2015.1064206.

[12] Longo VD, Antebi A, Bartke A, Barzilai N, Brown-Borg HM, Caruso C, Curiel TJ, de Cabo R, Franceschi C, Gems D, Ingram DK, Johnson TE, Kennedy BK, Kenyon C, Klein S, Kopchick JJ, Lepperdinger G, Madeo F, Mirisola MG, Mitchell JR, Passarino G, Rudolph KL, Sedivy JM, Shadel GS, Sinclair DA, Spindler SR, Suh Y, Vijg J, Vinciguerra M, and Fontana L 2015. Interventions to Slow Aging in Humans: Are We Ready? Aging Cell 14(4): 497–510. doi: 10.1111/acel.12338.

[13] Pietrocola F, Galluzzi L, Bravo-San Pedro JM, Madeo F, and Kroemer G 2015. Acetyl coenzyme A: a central metabolite and second messenger. Cell Metab 21(6): 805–821. doi: 10.1016/j.cmet.2015.05.014.

[14] Carmona-Gutierrez D, Zimmermann A, and Madeo F 2015. A molecular mechanism for lipophagy regulation in the liver. Hepatology 61(6): 1781–1783. doi: 10.1002/hep.27738.

[15] Libiseller G, Dvorzak M, Kleb U, Gander E, Eisenberg T, Madeo F, Neumann S, Trausinger G, Sinner F, Pieber T, and Magnes C 2015. IPO: a tool for automated

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optimization of XCMS parameters. BMC Bioinformatics 16: 118. doi: 10.1186/s12859-015-0562-8.

[16] Niso-Santano M, Malik SA, Pietrocola F, Bravo-San Pedro JM, Mariño G, Cianfanelli V, Ben-Younès A, Troncoso R, Markaki M, Sica V, Izzo V, Chaba K, Bauvy C, Dupont N, Kepp O, Rockenfeller P, Wolinski H, Madeo F, Lavandero S, Codogno P, Harper F, Pierron G, Tavernarakis N, Cecconi F, Maiuri MC, Galluzzi L, and Kroemer G 2015. Unsaturated fatty acids induce non-canonical autophagy. EMBO J 34(8): 1025–1041. doi: 10.15252/embj.201489363.

[17] Braun RJ, Sommer C, Leibiger C, Gentier RJG, Dumit VI, Paduch K, Eisenberg T, Habernig L, Trausinger G, Magnes C, Pieber T, Sinner F, Dengjel J, van Leeuwen FW, Kroemer G, and Madeo F 2015. Accumulation of Basic Amino Acids at Mitochondria Dictates the Cytotoxicity of Aberrant Ubiquitin. Cell Rep doi: 10.1016/j.celrep.2015.02.009.

[18] Rockenfeller P, Koska M, Pietrocola F, Minois N, Knittelfelder O, Sica V, Franz J, Carmona-Gutierrez D, Kroemer G, and Madeo F 2015. Phosphatidylethanolamine positively regulates autophagy and longevity. Cell Death Differ 22(3): 499–508. doi: 10.1038/cdd.2014.219.

[19] Pietrocola F, Lachkar S, Enot DP, Niso-Santano M, Bravo-San Pedro JM, Sica V, Izzo V, Maiuri MC, Madeo F, Mariño G, and Kroemer G 2015. Spermidine induces autophagy by inhibiting the acetyltransferase EP300. Cell Death Differ 22(3): 509–516. doi: 10.1038/cdd.2014.215.

[20] Hine C, Harputlugil E, Zhang Y, Ruckenstuhl C, Lee BC, Brace L, Longchamp A, Treviño-Villarreal JH, Mejia P, Ozaki CK, Wang R, Gladyshev VN, Madeo F, Mair WB, and Mitchell JR 2015. Endogenous hydrogen sulfide production is essential for dietary restriction benefits. Cell 160(1–2): 132–144. doi: 10.1016/j.cell.2014.11.048.

[21] Madeo F, Zimmermann A, Maiuri MC, and Kroemer G 2015. Essential role for autophagy in life span extension. J Clin Invest 125(1): 85–93. doi: 10.1172/JCI73946.

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[77] Braun RJ, Sommer C, Carmona-Gutierrez D, Khoury CM, Ring J, Büttner S, and Madeo F 2011. Neurotoxic 43-kDa TAR DNA-binding protein (TDP-43) triggers mitochondrion-dependent programmed cell death in yeast. J Biol Chem 286(22): 19958–19972. doi: 10.1074/jbc.M110.194852.

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[81] Aflaki E, Radovic B, Chandak PG, Kolb D, Eisenberg T, Ring J, Fertschai I, Uellen A, Wolinski H, Kohlwein S-D, Zechner R, Levak-Frank S, Sattler W, Graier WF, Malli R, Madeo F, and Kratky D 2011. Triacylglycerol accumulation activates the mitochondrial apoptosis pathway in macrophages. J Biol Chem 286(9): 7418–7428. doi: 10.1074/jbc.M110.175703.

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[154] Vahsen N, Candé C, Brière J-J, Bénit P, Joza N, Larochette N, Mastroberardino PG, Pequignot MO, Casares N, Lazar V, Feraud O, Debili N, Wissing S, Engelhardt S, Madeo F, Piacentini M, Penninger JM, Schägger H, Rustin P, and Kroemer G 2004. AIF deficiency compromises oxidative phosphorylation. EMBO J 23(23): 4679–4689. doi: 10.1038/sj.emboj.7600461.

[155] Wissing S, Ludovico P, Herker E, Büttner S, Engelhardt SM, Decker T, Link A, Proksch A, Rodrigues F, Corte-Real M, Fröhlich K-U, Manns J, Candé C, Sigrist SJ, Kroemer G, and Madeo F 2004. An AIF orthologue regulates apoptosis in yeast. J Cell Biol 166(7): 969–974. doi: 10.1083/jcb.200404138.

[156] Wadskog I, Maldener C, Proksch A, Madeo F, and Adler L 2004. Yeast lacking the SRO7/SOP1-encoded tumor suppressor homologue show increased susceptibility to apoptosis-like cell death on exposure to NaCl stress. Mol Biol Cell 15(3): 1436–1444. doi: 10.1091/mbc.E03-02-0114.

[157] Herker E, Jungwirth H, Lehmann KA, Maldener C, Fröhlich K-U, Wissing S, Büttner S, Fehr M, Sigrist S, and Madeo F 2004. Chronological aging leads to apoptosis in yeast. J Cell Biol 164(4): 501–507. doi: 10.1083/jcb.200310014.

[158] Swan LE, Wichmann C, Prange U, Schmid A, Schmidt M, Schwarz T, Ponimaskin E, Madeo F, Vorbrüggen G, and Sigrist SJ 2004. A glutamate receptor-interacting protein homolog organizes muscle guidance in Drosophila. Genes Dev 18(2): 223–237. doi: 10.1101/gad.287604.

[159] Mazzoni C, Mancini P, Madeo F, Palermo V, and Falcone C 2003. A Kluyveromyces lactis mutant in the essential gene KlLSM4 shows phenotypic markers of apoptosis. FEMS Yeast Res 4(1): 29–35.

[160] Mazzoni C, Mancini P, Verdone L, Madeo F, Serafini A, Herker E, and Falcone C 2003. A truncated form of KlLsm4p and the absence of factors involved in mRNA decapping trigger apoptosis in yeast. Mol Biol Cell 14(2): 721–729. doi: 10.1091/mbc.E02-05-0258.

[161] Madeo F and Fröhlich KU (2002). Comment on Severin, F.F., and Hyman, A.A. 2002. Pheromone induces programmed cell death in S. cerevisiae. Curr. Biol. 12, R233-R235. Curr Biol 12(13): R445.

[162] Madeo F, Engelhardt S, Herker E, Lehmann N, Maldener C, Proksch A, Wissing S, and Fröhlich K-U 2002. Apoptosis in yeast: a new model system with applications in cell biology and medicine. Curr Genet 41(4): 208–216. doi: 10.1007/s00294-002-0310-2.

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[163] Madeo F, Herker E, Maldener C, Wissing S, Lächelt S, Herlan M, Fehr M, Lauber K, Sigrist SJ, Wesselborg S, and Fröhlich KU 2002. A caspase-related protease regulates apoptosis in yeast. Mol Cell 9(4): 911–917.

[164] Fröhlich KU and Madeo F 2001. Apoptosis in yeast: a new model for aging research. Exp Gerontol 37(1): 27–31.

[165] Ligr M, Velten I, Fröhlich E, Madeo F, Ledig M, Fröhlich KU, Wolf DH, and Hilt W 2001. The proteasomal substrate Stm1 participates in apoptosis-like cell death in yeast. Mol Biol Cell 12(8): 2422–2432.

[166] Laun P, Pichova A, Madeo F, Fuchs J, Ellinger A, Kohlwein S, Dawes I, Fröhlich KU, and Breitenbach M 2001. Aged mother cells of Saccharomyces cerevisiae show markers of oxidative stress and apoptosis. Mol Microbiol 39(5): 1166–1173.

[167] Fröhlich KU and Madeo F 2000. Apoptosis in yeast--a monocellular organism exhibits altruistic behaviour. FEBS Lett 473(1): 6–9.

[168] Madeo F, Fröhlich E, Ligr M, Grey M, Sigrist SJ, Wolf DH, and Fröhlich KU 1999. Oxygen stress: a regulator of apoptosis in yeast. J Cell Biol 145(4): 757–767.

[169] Ligr M, Madeo F, Fröhlich E, Hilt W, Fröhlich KU, and Wolf DH 1998. Mammalian Bax triggers apoptotic changes in yeast. FEBS Lett 438(1–2): 61–65.

[170] Madeo F, Schlauer J, Zischka H, Mecke D, and Fröhlich KU 1998. Tyrosine phosphorylation regulates cell cycle-dependent nuclear localization of Cdc48p. Mol Biol Cell 9(1): 131–141.

[171] Madeo F, Schlauer J, and Fröhlich KU 1997. Identification of the regions of porcine VCP preventing its function in Saccharomyces cerevisiae. Gene 204(1–2): 145–151.

[172] Madeo F, Fröhlich E, and Fröhlich KU 1997. A yeast mutant showing diagnostic markers of early and late apoptosis. J Cell Biol 139(3): 729–734.

Selected oral presentations 2016 Invitation of behalf of the Swedish Royal Academy of Science, Goetheborg, Sweden 2014 Keystone Symposium on Autophagy, Austin, TX, USA 2012 EMBO conference on “Programmed Cell Death in Model Organisms” Ein Gedi, Israel 2010 Keystone Symposium on Cell Death Pathways. Vancouver, Canada 2010 Gordon Research Conference “Aging”. Les Diablerets, Switzerland

Patents

Baekelandt V., Buettner S., Madeo F., Winderickx J., (2014). Alpha Synuclein Toxicity. US2014093494 (A1).

Winderickx J., Van den Berghe G., Gunst J., Vanhorebeek I., Langouche L., Eisenberg T., Madeo F., Magnes C., Sinner F., (2012), Methods and Preparations for Protecting Critically Ill Patients, US2012015901 (A1).

Madeo F., Eisenberg T., Pieber T., Magnes C., Sinner F., Winderickx J., Van den Berghe G., Gunst J., (2012), Methods and Compostions for Diagnosing Medical Conditions, WO2012007531 (A2); WO2012007531 (A3).

Eisenberg T., Rockenfeller P., Madeo F., Monois N., Kroemer G., Winderickx J., (2010), Activators of the Autophagic Pathway, NL1036427 (C).

Baekelandt V., Buettner S., Gerard M., Madeo F., Windericks J., (2010), Synucleinophaties, WO2010094090 (A3); WO2010094090 (A2).

Winderickx J., Eisenberg T., Kroemer G., Madeo F., Minois N., Rockenfeller P., (2010), Activators of the Autophagic Pathway, WO2010081204 (A2); WO2010081204 (A3).

Froehlich KU., Madeo F., (2000) Rapid Screening Assay for Apoptosis-modulating Substances Comprises Determining the Effects of Substances on Yeasts, DE19842612 (A1).

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Curriculum Vitae – Assoz. Prof. Dr. rer. nat. Monika Oberer Institute of Molecular Biosciences, University of Graz, Humboldtstrasse 50, 3rd floor, 8010 Graz, Austria, Phone: +43 (0) 316 380 5483, E-Mail: [email protected] Year/ Place of Birth *1973/ Hartberg, Austria Nationality Austrian Orcid ID http://orcid.org/0000-0003-2525-9513 No of Publications 40 (13 first and/or corresponding) h-Index 19 (Scopus, June 2016) Supervised Ph.D. Students since 2007: 13 (6 finished, 7 ongoing) Key words Structure and function of proteins, protein-protein/DNA/lipid interaction,

lipid metabolism, lipases, NMR spectroscopy, X-ray crystallography, biophysics, biochemistry


since 2014 Associate Professor, Institute of Molecular Biosciences (IMB), University of Graz, Austria

2013 Habilitation (‚venia docendi‘), University of Graz 2008-2011 University Assistant at IMB, University of Graz since 2008 Project Leader (GOLD-III, DK Molecular Enzymology, SFB Lipotox) 2006-2008 Co-Project Leader in ‘Genomics of Lipid-associated Disorders’ –

GOLD-II; Institute of Chemistry, University of Graz 2002-2006 Post-doctoral Research Fellow, Laboratory of Prof. G. Wagner, BCMP,

Harvard Medical School, Boston, MA, USA Education 1998-2002 Ph.D. thesis, Institute of Chemistry, University of Graz, Austria,

Graduation Promotio sub auspiciis praesidentis rei publicae Supervisor: Prof. Dr. C. Kratky

1997 Diploma thesis, Center of Molecular Genetics, University of California San Diego (UCSD), La Jolla, USA

1995-96 Studies in Biochemistry and Chemistry, Western Washington University, Bellingham, USA

1991-1997 Studies of Chemistry, Karl-Franzens University (KFU) Graz, Austria Honors ▪ Awards 2015 Promotion Award for Scientific Achievements (Förderungungspreis)

sponsored by the State of Styria, Austria 2015 Journal of Structural and Functional Genomics Poster Award, ICSG,

Rehovot, Israel 2013 Kardinal-Innitzer-Foerderungspreis (Cardinal-Innitzer Promotion Award),

Kardinal-Innitzer-Studienfonds, Vienna. 2008 Award for Excellent Scientific Achievements, University of Graz 2005-2006 Max-Kade Fellowship, awarded by the Austrian Academy of Sciences and the Max-Kade Foundation, New York, USA 2003-2005 Erwin-Schrödinger Fellowship, awarded by the Austrian Science Fund (FWF) Career related activities since 2011 Editorial board member PLosOne since 2010 Reviewer of peer-reviewed Journals (Cell Metabolism, e-Life, BBA, JBC,

Biochemistry, PLosOne, JLR, Journal of Structural Biology, etc) since 2010 Member of scientific and organizing committee of meetings (e.g.

Austrian Bio-NMR Meeting, International Graz Symposium on Lipid and Membrane Biology, Graz, Austria)


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Funding Org.

Project Number


Funding Period

University support

Incentive Förderung: S-Layer

Proteins 36 2016

FWF/ University support

F3016 SFB Lipotox, Subproject P16;

Priority Research Program 2nd and 3rd Funding Period

577 2011-2017


W9 DK Molecular Enzymology 322 2014-2017

Foundation Leducq

12CVD04

Transatlantic Network on Triglycerides — Triglyceride Metabolism in Obesity and

Cardiovascular Disease

191 2013-2017

NAWI Graz

N/A

Unraveling the secrets of the “lipolysome”: Characterization of lipolytic proteins and its protein-

protein complexes

90 2012-2015

FWF P24857 Structure, dynamics, and substrate specificity of monoglyceride lipases

348 2012-2017



FWF P22170 Functional Characterization of CGI-

58 293 2010-2015

Publications [1] Heier, C., Taschler, U., Radulovic, M., Aschauer, P., Eichmann, T.O., Grond, S., Wolinski,

H., Oberer, M., Zechner, R., Kohlwein, S.D., Zimmermann, R. Monoacylglycerol Lipases Act as Evolutionarily Conserved Regulators of Non-oxidative Ethanol Metabolism J Biol Chem 2016, 291 (22), 11865-11875. 10.1074/jbc.M115.705541

[2] Fong, L.G., Young, S.G., Beigneux, A.P., Bensadoun, A., Oberer, M., Jiang, H., Ploug, M. GPIHBP1 and Plasma Triglyceride Metabolism Trends Endocrinol Metab 2016, 27 (7), 455-469. 10.1016/j.tem.2016.04.013

[3] Aschauer, P., Rengachari, S., Lichtenegger, J., Schittmayer, M., Das, K.M., Mayer, N., Breinbauer, R., Birner-Gruenberger, R., Gruber, C.C., Zimmermann, R., Gruber, K., Oberer, M. Crystal structure of the Saccharomyces cerevisiae monoglyceride lipase Yju3p Biochim Biophys Acta 2016, 1861 (5), 462-470. 10.1016/j.bbalip.2016.02.005

[4] Sahu-Osen, A., Montero-Moran, G., Schittmayer, M., Fritz, K., Dinh, A., Chang, Y.F., McMahon, D., Boeszoermenyi, A., Cornaciu, I., Russell, D., Oberer, M., Carman, G.M., Birner-Gruenberger, R., Brasaemle, D.L. CGI-58/ABHD5 is phosphorylated on Ser239 by protein kinase A: control of subcellular localization J Lipid Res 2015, 56 (1), 109-121. 10.1194/jlr.M055004

[5] Rengachari, S., Aschauer, P., Sturm, C., Oberer, M. Purification, crystallization and preliminary X-ray diffraction analysis of a soluble variant of the monoglyceride lipase Yju3p from the yeast Saccharomyces cerevisiae Acta Crystallogr F Struct Biol Commun 2015, 71 (Pt 2), 243-246. 10.1107/S2053230X15001557

[6] Hofer, P., Boeszoermenyi, A., Jaeger, D., Feiler, U., Arthanari, H., Mayer, N., Zehender, F., Rechberger, G., Oberer, M., Zimmermann, R., Lass, A., Haemmerle, G., Breinbauer, R., Zechner, R., Preiss-Landl, K. Fatty Acid-binding Proteins Interact with Comparative

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Gene Identification-58 Linking Lipolysis with Lipid Ligand Shuttling J Biol Chem 2015, 290 (30), 18438-18453. 10.1074/jbc.M114.628958

[7] Heier, C., Radner, F.P., Moustafa, T., Schreiber, R., Grond, S., Eichmann, T.O., Schweiger, M., Schmidt, A., Cerk, I.K., Oberer, M., Theussl, H.C., Wojciechowski, J., Penninger, J.M., Zimmermann, R., Zechner, R. G0/G1 Switch Gene 2 Regulates Cardiac Lipolysis J Biol Chem 2015, 290 (43), 26141-26150. 10.1074/jbc.M115.671842

[8] Boeszoermenyi, A., Nagy, H.M., Arthanari, H., Pillip, C.J., Lindermuth, H., Luna, R.E., Wagner, G., Zechner, R., Zangger, K., Oberer, M. Structure of a CGI-58 Motif Provides the Molecular Basis of Lipid Droplet Anchoring J Biol Chem 2015, 290 (44), 26361-26372. 10.1074/jbc.M115.682203

[9] Beigneux, A.P., Fong, L.G., Bensadoun, A., Davies, B.S., Oberer, M., Gardsvoll, H., Ploug, M., Young, S.G. GPIHBP1 missense mutations often cause multimerization of GPIHBP1 and thereby prevent lipoprotein lipase binding Circ Res 2015, 116 (4), 624-632. 10.1161/CIRCRESAHA.116.305085

[10] Adeyo, O., Oberer, M., Ploug, M., Fong, L.G., Young, S.G., Beigneux, A.P. Heterogeneity in the properties of mutant secreted lymphocyte antigen 6/urokinase receptor-related protein 1 (SLURP1) in Mal de Meleda Br J Dermatol 2015, 173 (4), 1066-1069. 10.1111/bjd.13868

[11] Steinkellner, G., Gruber, C.C., Pavkov-Keller, T., Binter, A., Steiner, K., Winkler, C., Lyskowski, A., Schwamberger, O., Oberer, M., Schwab, H., Faber, K., Macheroux, P., Gruber, K. Identification of promiscuous ene-reductase activity by mining structural databases using active site constellations Nat Commun 2014, 5, 4150. 10.1038/ncomms5150

[12] Nagy, H.M., Paar, M., Heier, C., Moustafa, T., Hofer, P., Haemmerle, G., Lass, A., Zechner, R., Oberer, M., Zimmermann, R. Adipose triglyceride lipase activity is inhibited by long-chain acyl-coenzyme A Biochim Biophys Acta 2014, 1841 (4), 588-594. 10.1016/j.bbalip.2014.01.005

[13] Harper, S., Gratton, H.E., Cornaciu, I., Oberer, M., Scott, D.J., Emsley, J., Dreveny, I. Structure and catalytic regulatory function of ubiquitin specific protease 11 N-terminal and ubiquitin-like domains Biochemistry 2014, 53 (18), 2966-2978. 10.1021/bi500116x

[14] Cerk, I.K., Salzburger, B., Boeszoermenyi, A., Heier, C., Pillip, C., Romauch, M., Schweiger, M., Cornaciu, I., Lass, A., Zimmermann, R., Zechner, R., Oberer, M. A peptide derived from G0/G1 switch gene 2 acts as noncompetitive inhibitor of adipose triglyceride lipase J Biol Chem 2014, 289 (47), 32559-32570. 10.1074/jbc.M114.602599

[15] Boeszoermenyi, A., Schmidt, J.C., Cheeseman, I.M., Oberer, M., Wagner, G., Arthanari, H. Resonance assignments of the microtubule-binding domain of the C. elegans spindle and kinetochore-associated protein 1 Biomol NMR Assign 2014, 8 (2), 275-278. 10.1007/s12104-013-9499-x

[16] Thiel, K., Heier, C., Haberl, V., Thul, P.J., Oberer, M., Lass, A., Jackle, H., Beller, M. The evolutionarily conserved protein CG9186 is associated with lipid droplets, required for their positioning and for fat storage J Cell Sci 2013, 126 (Pt 10), 2198-2212. 10.1242/jcs.120493

[17] Rengachari, S., Aschauer, P., Schittmayer, M., Mayer, N., Gruber, K., Breinbauer, R., Birner-Gruenberger, R., Dreveny, I., Oberer, M. Conformational plasticity and ligand binding of bacterial monoacylglycerol lipase J Biol Chem 2013, 288 (43), 31093-31104. 10.1074/jbc.M113.491415

[18] Schweiger, M., Paar, M., Eder, C., Brandis, J., Moser, E., Gorkiewicz, G., Grond, S., Radner, F.P., Cerk, I., Cornaciu, I., Oberer, M., Kersten, S., Zechner, R., Zimmermann, R., Lass, A. G0/G1 switch gene-2 regulates human adipocyte lipolysis by affecting activity and localization of adipose triglyceride lipase J Lipid Res 2012, 53 (11), 2307-2317. 10.1194/jlr.M027409

[19] Schmidt, J.C., Arthanari, H., Boeszoermenyi, A., Dashkevich, N.M., Wilson-Kubalek, E.M., Monnier, N., Markus, M., Oberer, M., Milligan, R.A., Bathe, M., Wagner, G., Grishchuk, E.L., Cheeseman, I.M. The kinetochore-bound Ska1 complex tracks depolymerizing microtubules and binds to curved protofilaments Dev Cell 2012, 23 (5), 968-980. 10.1016/j.devcel.2012.09.012

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[20] Rengachari, S., Bezerra, G.A., Riegler-Berket, L., Gruber, C.C., Sturm, C., Taschler, U., Boeszoermenyi, A., Dreveny, I., Zimmermann, R., Gruber, K., Oberer, M. The structure of monoacylglycerol lipase from Bacillus sp. H257 reveals unexpected conservation of the cap architecture between bacterial and human enzymes Biochim Biophys Acta 2012, 1821 (7), 1012-1021. 10.1016/j.bbalip.2012.04.006

[21] Kumari, M., Schoiswohl, G., Chitraju, C., Paar, M., Cornaciu, I., Rangrez, A.Y., Wongsiriroj, N., Nagy, H.M., Ivanova, P.T., Scott, S.A., Knittelfelder, O., Rechberger, G.N., Birner-Gruenberger, R., Eder, S., Brown, H.A., Haemmerle, G., Oberer, M., Lass, A., Kershaw, E.E., Zimmermann, R., Zechner, R. Adiponutrin functions as a nutritionally regulated lysophosphatidic acid acyltransferase Cell Metab 2012, 15 (5), 691-702. 10.1016/j.cmet.2012.04.008

[22] Oberer, M., Boeszoermenyi, A., Nagy, H.M., Zechner, R. Recent insights into the structure and function of comparative gene identification-58 Curr Opin Lipidol 2011, 22 (3), 149-158. 10.1097/MOL.0b013e328346230e

[23] Lass, A., Zimmermann, R., Oberer, M., Zechner, R. Lipolysis - a highly regulated multi-enzyme complex mediates the catabolism of cellular fat stores Prog Lipid Res 2011, 50 (1), 14-27. 10.1016/j.plipres.2010.10.004

[24] Cornaciu, I., Boeszoermenyi, A., Lindermuth, H., Nagy, H.M., Cerk, I.K., Ebner, C., Salzburger, B., Gruber, A., Schweiger, M., Zechner, R., Lass, A., Zimmermann, R., Oberer, M. The minimal domain of adipose triglyceride lipase (ATGL) ranges until leucine 254 and can be activated and inhibited by CGI-58 and G0S2, respectively PLoS One 2011, 6 (10), e26349. 10.1371/journal.pone.0026349

[25] Heier, C., Taschler, U., Rengachari, S., Oberer, M., Wolinski, H., Natter, K., Kohlwein, S.D., Leber, R., Zimmermann, R. Identification of Yju3p as functional orthologue of mammalian monoglyceride lipase in the yeast Saccharomycescerevisiae Biochim Biophys Acta 2010, 1801 (9), 1063-1071. 10.1016/j.bbalip.2010.06.001

[26] Gruber, A., Cornaciu, I., Lass, A., Schweiger, M., Poeschl, M., Eder, C., Kumari, M., Schoiswohl, G., Wolinski, H., Kohlwein, S.D., Zechner, R., Zimmermann, R., Oberer, M. The N-terminal region of comparative gene identification-58 (CGI-58) is important for lipid droplet binding and activation of adipose triglyceride lipase J Biol Chem 2010, 285 (16), 12289-12298. 10.1074/jbc.M109.064469

[27] Marintchev, A., Edmonds, K.A., Marintcheva, B., Hendrickson, E., Oberer, M., Suzuki, C., Herdy, B., Sonenberg, N., Wagner, G. Topology and regulation of the human eIF4A/4G/4H helicase complex in translation initiation Cell 2009, 136 (3), 447-460. 10.1016/j.cell.2009.01.014

[28] Kienesberger, P.C., Oberer, M., Lass, A., Zechner, R. Mammalian patatin domain containing proteins: a family with diverse lipolytic activities involved in multiple biological functions J Lipid Res 2009, 50 Suppl, S63-68. 10.1194/jlr.R800082-JLR200

[29] Schweiger, M., Schoiswohl, G., Lass, A., Radner, F.P., Haemmerle, G., Malli, R., Graier, W., Cornaciu, I., Oberer, M., Salvayre, R., Fischer, J., Zechner, R., Zimmermann, R. The C-terminal region of human adipose triglyceride lipase affects enzyme activity and lipid droplet binding J Biol Chem 2008, 283 (25), 17211-17220. 10.1074/jbc.M710566200

[30] Lindqvist, L., Oberer, M., Reibarkh, M., Cencic, R., Bordeleau, M.E., Vogt, E., Marintchev, A., Tanaka, J., Fagotto, F., Altmann, M., Wagner, G., Pelletier, J. Selective pharmacological targeting of a DEAD box RNA helicase PLoS One 2008, 3 (2), e1583. 10.1371/journal.pone.0001583

[31] Oberer, M., Zangger, K., Gruber, K., Keller, W. The solution structure of ParD, the antidote of the ParDE toxin antitoxin module, provides the structural basis for DNA and toxin binding Protein Sci 2007, 16 (8), 1676-1688. 10.1110/ps.062680707

[32] Madl, T., Van Melderen, L., Mine, N., Respondek, M., Oberer, M., Keller, W., Khatai, L., Zangger, K. Structural basis for nucleic acid and toxin recognition of the bacterial antitoxin CcdA J Mol Biol 2006, 364 (2), 170-185. 10.1016/j.jmb.2006.08.082

[33] Bordeleau, M.E., Mori, A., Oberer, M., Lindqvist, L., Chard, L.S., Higa, T., Belsham, G.J., Wagner, G., Tanaka, J., Pelletier, J. Functional characterization of IRESes by an inhibitor of the RNA helicase eIF4A Nat Chem Biol 2006, 2 (4), 213-220. 10.1038/nchembio776

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[34] Bordeleau, M.E., Cencic, R., Lindqvist, L., Oberer, M., Northcote, P., Wagner, G., Pelletier, J. RNA-mediated sequestration of the RNA helicase eIF4A by Pateamine A inhibits translation initiation Chem Biol 2006, 13 (12), 1287-1295. 10.1016/j.chembiol.2006.10.005

[35] Oberer, M., Marintchev, A., Wagner, G. Structural basis for the enhancement of eIF4A helicase activity by eIF4G Genes Dev 2005, 19 (18), 2212-2223. 10.1101/gad.1335305

[36] Zangger, K., Oberer, M., Keller, W., Sterk, H. X-filtering for a range of coupling constants: application to the detection of intermolecular NOEs J Magn Reson 2003, 160 (2), 97-106.

[37] Pavkov, T., Oberer, M., Egelseer, E.M., Sara, M., Sleytr, U.B., Keller, W. Crystallization and preliminary structure determination of the C-terminal truncated domain of the S-layer protein SbsC Acta Crystallogr D Biol Crystallogr 2003, 59 (Pt 8), 1466-1468.

[38] Oberer, M., Zangger, K., Prytulla, S., Keller, W. The anti-toxin ParD of plasmid RK2 consists of two structurally distinct moieties and belongs to the ribbon-helix-helix family of DNA-binding proteins Biochem J 2002, 361 (Pt 1), 41-47.

[39] Zangger, K., Oberer, M., Sterk, H. Pure-phase selective excitation in fast-relaxing systems J Magn Reson 2001, 152 (1), 48-56. 10.1006/jmre.2001.2401

[40] Oberer, M., Lindner, H., Glatter, O., Kratky, C., Keller, W. Thermodynamic properties and DNA binding of the ParD protein from the broad host-range plasmid RK2/RP4 killing system Biol Chem 1999, 380 (12), 1413-1420. 10.1515/BC.1999.181

Selected oral presentations 2016 “The cap makes the difference: Structural evidence for conserved cap architecture and

conformational plasticity in monoacylglycerol lipases”. Brno. 8th Conference Chemistry towards Biology

2016 “Structure of a CGI-58 Motif Provides the Molecular Basis of Lipid Droplet Anchoring” First Austrian Bio-NMR Meeting, Graz, Austria

2014 “Structural Biology in Lipid Metabolism” Young Investigators in Lipid Science, Düsseldorf, Germany

2013 „Structural Investigations in Lipid Metabolism“, X. H.P. Kaufmann-Tage (German Society for Fat Science, DGF), Heidelberg, Germany

2011 “Cap architecture conservation in monoglyceride lipases from bacteria to humans”. German Crystallographic Society Meeting (DGK), Salzburg, Austria

2010 “The N-Terminal Region of Comparative Gene Identification-58 (Cgi-58) is Important for Lipid Droplet Binding and Activation of Adipose Triglyceride Lipase”, Gordon Research Conference, GRC Lipid and Lipoprotein Metabolism in Health and Disease, Waterville Valley, USA

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Curriculum Vitae – Assoz. Prof. Dr. rer. nat. Stefan Schild Institute of Molecular Biosciences, University of Graz, Humboldtstrasse 50, 1st floor, 8010 Graz, Austria, Phone: +43 (0) 316 380 1970, E-Mail: [email protected] Year/ Place of Birth *1976/ Ingolstadt, Germany Nationality German Orcid ID http://orcid.org/ 0000-0001-7842-0177 No of Publications 35 (22 first and/or corresponding) h-Index 16 (Scopus, Nov, 2016) Supervised Ph.D. Students since 2011: 3 finished, 5 ongoing Key words bacterial pathogens, biofilm, transmission, nutrient aquisition, virulence,

nucleases, lipid transporter, outer membrane, outer membrane vesicles, colonization


since 2011 Associate professor, Institute of Molecular Biosciences, University of Graz, Austria

2011 Habilitation in “Microbiology” 2010 - 2011 Assistant professor, Institute of Molecular Biosciences, University of

Graz, Austria 2008 - 2010 University Assistant, Institute of Molecular Biosciences, University of

Graz, Austria 2005 - 2008 Howard Hughes Medical Institute (HHMI) research associate in the lab

of Dr. Andrew Camilli, Department of Molecular Biology & Microbiology, Tufts University, Boston, MA

Education 2002 - 2005 PhD thesis (very good)

University of Wuerzburg, Germany, Supervisor: Prof. Dr. J. Reidl 1996 - 2002 Study in Biology (with distinction), University of Wuerzburg, Germany Supervisor diploma thesis: Prof. Dr. J. Hacker Honors ▪ Awards 2014 Innovation award, Science Park Graz 2011 Travel award from the Land Steiermark 2009 Publication award from the Heinrich-Jörg Stiftung 2002 – 2005 PhD fellowship from the “Studienstiftung des deutschen Volkes” Career related activities since 2013 member of the ethic commission for animal research, Institute of

Molecular Biosciences, University of Graz, Austria since 2011 co-representative for biological safety BSL-2 and genetically modified

organisms (GMOs), Institute of Molecular Biosciences, University of Graz, Austria

since 2008 Coordinator of animal research for the Dept. of Microbiology, Institute of Molecular Biosciences, University of Graz, Austria

since 2000 trained in special courses on laboratory animals certified by GV-Solas/ FELASA, category B

1997 - 2002 Member of the students council of the faculty of Biology, University of Wuerzburg, Germany

1998 - 1999 Speaker of the students council


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Funding Org.

Project Number

Research Topic Amount funded [kEuro]

Funding Period

BioTechMed/ University Support

-

Immunomodulatory and Antiproliferative Bioactivities of the Bacterial Secretome

in Gastrointestinal Disease and Therapy (Coordinator & PI)

186 starting in

2017

FWF P 27654 Characterization of Vibrio cholerae

biofilms 329 2016-2021

FFG – Basis project

853729 “Immunotherapy against P. acnes for treatment of acne vulgaris and

implant associated infections” 65/ year since 2016



FWF P 25691 Outer membrane vesicles of

enteric pathogens 306 2013-2018

FWF P 22986 Gene regulation in dynamic

biofilms 282 2011-2015



Publications [1] Vorkapic, D., Pressler, K., Schild, S. Multifaceted roles of extracellular DNA in bacterial

physiology Current genetics 2016, 62 (1), 71-79. 10.1007/s00294-015-0514-x [2] Roier, S., Zingl, F.G., Cakar, F., Durakovic, S., Kohl, P., Eichmann, T.O., Klug, L.,

Gadermaier, B., Weinzerl, K., Prassl, R., Lass, A., Daum, G., Reidl, J., Feldman, M.F., Schild, S. A novel mechanism for the biogenesis of outer membrane vesicles in Gram-negative bacteria Nature communications 2016, 7, 10515. 10.1038/ncomms10515

[3] Pressler, K., Vorkapic, D., Lichtenegger, S., Malli, G., Barilich, B.P., Cakar, F., Zingl, F.G., Reidl, J., Schild, S. AAA+ proteases and their role in distinct stages along the Vibrio cholerae lifecycle Int J Med Microbiol 2016, 306 (6), 452-462. 10.1016/j.ijmm.2016.05.013

[4] Kim, H.M., Colaiacovo, M.P. CRISPR-Cas9-Guided Genome Engineering in C. elegans Current protocols in molecular biology 2016, 115, 31 37 31-31 37 18. 10.1002/cpmb.7

[5] Gumpenberger, T., Vorkapic, D., Zingl, F.G., Pressler, K., Lackner, S., Seper, A., Reidl, J., Schild, S. Nucleoside uptake in Vibrio cholerae and its role in the transition fitness from host to environment Mol Microbiol 2016, 99 (3), 470-483. 10.1111/mmi.13143

[6] Salem, W., Leitner, D.R., Zingl, F.G., Schratter, G., Prassl, R., Goessler, W., Reidl, J., Schild, S. Antibacterial activity of silver and zinc nanoparticles against Vibrio cholerae and enterotoxic Escherichia coli Int J Med Microbiol 2015, 305 (1), 85-95. 10.1016/j.ijmm.2014.11.005

[7] Roier, S., Blume, T., Klug, L., Wagner, G.E., Elhenawy, W., Zangger, K., Prassl, R., Reidl, J., Daum, G., Feldman, M.F., Schild, S. A basis for vaccine development: Comparative characterization of Haemophilus influenzae outer membrane vesicles Int J Med Microbiol 2015, 305 (3), 298-309. 10.1016/j.ijmm.2014.12.005

[8] Leitner, D.R., Lichtenegger, S., Temel, P., Zingl, F.G., Ratzberger, D., Roier, S., Schild-Prufert, K., Feichter, S., Reidl, J., Schild, S. A combined vaccine approach against Vibrio

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cholerae and ETEC based on outer membrane vesicles Front Microbiol 2015, 6, 823. 10.3389/fmicb.2015.00823

[9] Seper, A., Pressler, K., Kariisa, A., Haid, A.G., Roier, S., Leitner, D.R., Reidl, J., Tamayo, R., Schild, S. Identification of genes induced in Vibrio cholerae in a dynamic biofilm system Int J Med Microbiol 2014, 304 (5-6), 749-763. 10.1016/j.ijmm.2014.05.011

[10] Schneditz, G., Rentner, J., Roier, S., Pletz, J., Herzog, K.A., Bucker, R., Troeger, H., Schild, S., Weber, H., Breinbauer, R., Gorkiewicz, G., Hogenauer, C., Zechner, E.L. Enterotoxicity of a nonribosomal peptide causes antibiotic-associated colitis Proceedings of the National Academy of Sciences of the United States of America 2014, 111 (36), 13181-13186. 10.1073/pnas.1403274111

[11] Lichtenegger, S., Bina, I., Roier, S., Bauernfeind, S., Keidel, K., Schild, S., Anthony, M., Reidl, J. Characterization of lactate utilization and its implication on the physiology of Haemophilus influenzae Int J Med Microbiol 2014, 304 (3-4), 490-498. S1438-42 10.1016/j.ijmm.2014.02.010

[12] Seper, A., Hosseinzadeh, A., Gorkiewicz, G., Lichtenegger, S., Roier, S., Leitner, D.R., Rohm, M., Grutsch, A., Reidl, J., Urban, C.F., Schild, S. Vibrio cholerae Evades Neutrophil Extracellular Traps by the Activity of Two Extracellular Nucleases PLoS Pathog 2013, 9 (9), e1003614. 10.1371/journal.ppat.1003614

[13] Roier, S., Fenninger, J.C., Leitner, D.R., Rechberger, G.N., Reidl, J., Schild, S. Immunogenicity of Pasteurella multocida and Mannheimia haemolytica outer membrane vesicles Int J Med Microbiol 2013, 303 (5), 247-256. S1438-4221(13)00065-9 10.1016/j.ijmm.2013.05.001

[14] Moisi, M., Lichtenegger, S., Tutz, S., Seper, A., Schild, S., Reidl, J. Characterizing the Hexose-6-Phosphate Transport System of Vibrio cholerae, a Utilization System for Carbon and Phosphate Sources J Bacteriol 2013, 195 (8), 1800-1808. 10.1128/JB.01952-12

[15] Leitner, D.R., Feichter, S., Schild-Prufert, K., Rechberger, G.N., Reidl, J., Schild, S. Lipopolysaccharide modifications of a cholera vaccine candidate based on outer membrane vesicles reduce endotoxicity and reveal the major protective antigen Infect Immun 2013, 81 (7), 2379-2393. 10.1128/IAI.01382-12

[16] Lees-Miller, R.G., Iwashkiw, J.A., Scott, N.E., Seper, A., Vinogradov, E., Schild, S., Feldman, M.F. A common pathway for O-linked protein-glycosylation and synthesis of capsule in Acinetobacter baumannii Mol Microbiol 2013, 89 (5), 816-830. 10.1111/mmi.12300

[17] Roier, S., Leitner, D.R., Iwashkiw, J., Schild-Prufert, K., Feldman, M.F., Krohne, G., Reidl, J., Schild, S. Intranasal immunization with nontypeable Haemophilus influenzae outer membrane vesicles induces cross-protective immunity in mice PLoS One 2012, 7 (8), e42664. 10.1371/journal.pone.0042664

[18] Iwashkiw, J.A., Seper, A., Weber, B.S., Scott, N.E., Vinogradov, E., Stratilo, C., Reiz, B., Cordwell, S.J., Whittal, R., Schild, S., Feldman, M.F. Identification of a general O-linked protein glycosylation system in Acinetobacter baumannii and its role in virulence and biofilm formation PLoS Pathog 2012, 8 (6), e1002758. 10.1371/journal.ppat.1002758

[19] Fengler, V.H., Boritsch, E.C., Tutz, S., Seper, A., Ebner, H., Roier, S., Schild, S., Reidl, J. Disulfide bond formation and ToxR activity in Vibrio cholerae PLoS One 2012, 7 (10), e47756. 10.1371/journal.pone.0047756

[20] Seper, A., Fengler, V.H., Roier, S., Wolinski, H., Kohlwein, S.D., Bishop, A.L., Camilli, A., Reidl, J., Schild, S. Extracellular nucleases and extracellular DNA play important roles in Vibrio cholerae biofilm formation Mol Microbiol 2011, 82 (4), 1015-1037. 10.1111/j.1365-2958.2011.07867.x

[21] Bishop, A.L., Schild, S., Patimalla, B., Klein, B., Camilli, A. Mucosal immunization with Vibrio cholerae outer membrane vesicles provides maternal protection mediated by antilipopolysaccharide antibodies that inhibit bacterial motility Infect. Immun. 2010, 78 (10), 4402-4420. 10.1128/IAI.00398-10

[22] Schild, S., Nelson, E.J., Bishop, A.L., Camilli, A. Characterization of Vibrio cholerae outer membrane vesicles as a candidate vaccine for cholera Infect Immun 2009, 77 (1), 472-484.

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[23] Moisi, M., Jenul, C., Butler, S.M., New, A., Tutz, S., Reidl, J., Klose, K.E., Camilli, A., Schild, S. A novel regulatory protein involved in motility of Vibrio cholerae J Bacteriol 2009, 191 (22), 7027-7038.

[24] Tamayo, R., Schild, S., Pratt, J.T., Camilli, A. Role of cyclic Di-GMP during el tor biotype Vibrio cholerae infection: characterization of the in vivo-induced cyclic Di-GMP phosphodiesterase CdpA Infect Immun 2008, 76 (4), 1617-1627.

[25] Song, T., Mika, F., Lindmark, B., Liu, Z., Schild, S., Bishop, A., Zhu, J., Camilli, A., Johansson, J., Vogel, J., Wai, S.N. A new Vibrio cholerae sRNA modulates colonization and affects release of outer membrane vesicles Mol Microbiol 2008, 70 (1), 100-111.

[26] Schild, S., Nelson, E.J., Camilli, A. Immunization with Vibrio cholerae outer membrane vesicles induces protective immunity in mice Infect Immun 2008, 76 (10), 4554-4563. 10.1128/IAI.00532-08

[27] Schild, S., Bishop, A.L., Camilli, A. Ins and Outs of Vibrio cholerae Microbe 2008, 3 (3), 131-136.

[28] Nelson, E.J., Chowdhury, A., Flynn, J., Schild, S., Bourassa, L., Shao, Y., LaRocque, R.C., Calderwood, S.B., Qadri, F., Camilli, A. Transmission of Vibrio cholerae is antagonized by lytic phage and entry into the aquatic environment PLoS Pathog 2008, 4 (10), e1000187.

[29] Schild, S., Tamayo, R., Nelson, E.J., Qadri, F., Calderwood, S.B., Camilli, A. Genes induced late in infection increase fitness of Vibrio cholerae after release into the environment Cell Host Microbe 2007, 2 (4), 264-277. 10.1016/j.chom.2007.09.004

[30] Berg, T., Schild, S., Reidl, J. Regulation of the chitobiose-phosphotransferase system in Vibrio cholerae Arch Microbiol 2007, 187 (6), 433-439. 10.1007/s00203-006-0207-4

[31] Schild, S., Lamprecht, A.K., Reidl, J. Molecular and functional characterization of O antigen transfer in Vibrio cholerae J Biol Chem 2005, 280 (27), 25936-25947. 10.1074/jbc.M501259200

[32] Schild, S., Lamprecht, A.K., Fourestier, C., Lauriano, C.M., Klose, K.E., Reidl, J. Characterizing lipopolysaccharide and core lipid A mutant O1 and O139 Vibrio cholerae strains for adherence properties on mucus-producing cell line HT29-Rev MTX and virulence in mice Int J Med Microbiol 2005, 295 (4), 243-251.

[33] Schild, S. Bedeutung der Lipopolysaccharidstrukturen bei pathogenen Vibrio cholerae Stämmen für die Ausbildung von Cholera und Abgrenzung zu Umweltisolaten Bayerischen Julius-Maximilians-Universität Würzburg, Würzburg, 2005.

[34] Schild, S., Reidl, J. Vibrio cholerae: Ursache und Wirkung BIOspektrum 2003, 4, 360-361.

[35] Nesper, J., Schild, S., Lauriano, C.M., Kraiss, A., Klose, K.E., Reidl, J. Role of Vibrio cholerae O139 surface polysaccharides in intestinal colonization Infect Immun 2002, 70 (11), 5990-5996.

[36] Nesper, J., Kraiss, A., Schild, S., Blass, J., Klose, K.E., Bockemuhl, J., Reidl, J. Comparative and genetic analyses of the putative Vibrio cholerae lipopolysaccharide core oligosaccharide biosynthesis (wav) gene cluster Infect Immun 2002, 70 (5), 2419-2433.

Patents

Roier S, Reidl J, Schild S. 2012. Vaccine against Pasteurellaceae. EP 2505208

Schild S, Nelson EJ, Camilli A. 2008. Cholera Vaccines. PCT/US08/879,292

Selected oral presentations 2015 „Bacterial Pathogenesis: Adaptation strategies, surface structures and therapeutic

interventions“. University of Hannover. October 2015 2015 "From outer membrane vesicles to nanoparticles: Therapeutic approaches to fight

bacterial infections". University of Stuttgart Hohenheim. May 2015

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2014 “They are out there – OMVs as vaccine candidates”. International Graduate School 1409, University Muenster. December 2014

2013 “OMVs - Vaccine against Pasteurellaceae”. Biovaria Munich. June 2013 2012 “There and Back again: A Vibrio's tale”. Research summit in Obergurgl of the DFG

research program „Host-Adapted Metabolism of Bacterial Pathogens“. October 2012 2011 “Outer membrane vesicles as vaccine candidates”. New York University – Dept. of

Medicine. September 2011. 2010 “Bacterial pathogenesis of the gastrointestinal tract: Vibrio cholerae and cholera”.

University of Wuerzburg - Symposium Infectious Diseases. March 2010 2010 “Microbial Pathogenesis: Vibrio cholerae and Clostridium difficile”. Copenhagen –

Symposium by MIMS. January 2010

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Curriculum Vitae – Univ.-Prof. Dipl.-Ing. Dr.rer.nat. Ulrich Stelzl Institute of Pharmaceutical Sciences (IPW), Department of Pharmaceutical Chemistry, University of Graz, Austria; Phone: +43 (0) 316 380 5397, E-Mail: [email protected] Year/ Place of Birth *1971/ Vienna, Austria Nationality Austrian Orcid ID http://orcid.org/0000-0003-2500-3585 No of Publications 44 (23 first and/or corresponding) h-Index 20 (Scopus, Nov, 2016) Supervised Ph.D. Students Since 2007: 8 finished, 1 ongoing Key words The group is focusing on the analysis of molecular interaction networks

with the aim to understand the dynamics of molecular networks underlying cellular processes related to human disease. Experimental functional genomics techniques, e.g. HTP Y2H screening, are utilized in combination with biochemical, cell biological and computational methods. Network biology offers a more comprehensive understanding of biology concomitantly improving the practice of medicine.


since Sept. 2015 Professor at the Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, University of Graz and BioTechMed-Graz, Austria

2007-2015 Max-Planck Research Group Leader (W2 position) at the Max-Planck Institute for Molecular Genetics (MPIMG), Berlin, Germany

Head of the Otto Warburg Laboratory “Molecular Interaction Networks” 2002-2007 Postdoctoral fellow / NGFN project leader in the AG Neuroproteomics

headed by Erich Wanker, Max-Delbrück Center for Molecular Medicine (MDC), Berlin-Buch, Germany

2001-2002 Research Fellow in the Laboratory of Dinshaw Patel at the Memorial Sloan Kettering Cancer Center (MSKCC), New York, USA

2000 Postdoctoral fellow in the AG Ribosomen, MPIMG, Berlin Education 1996-1999 Ph.D. thesis at the Max-Planck Institute Molecular Genetics, Berlin,

Germany in the AG Ribosomen under the supervision of Knud Nierhaus, Academic degree Dr. rer. nat., University of Vienna

1994-1995 Studies of Biochemistry at the ETH Zurich, Switzerland Diploma Thesis at the ETH Zurich, Laboratory for Organic Chemistry. 1990-1996 Studies of Technical Chemistry / Biochemistry at the TU Vienna

Graduated with honors, April 1996 at the TU Vienna Honors ▪ Awards 2008 Erwin Schrödinger Preis of the HGF (50 TEuro price money, shared) http://www.helmholtz.de/ueber_uns/forschungspreise/erwin_schroedinge

r_preis/ Career related activities Program committee member of ISMB 2015 and ISMB 2016 Special Section Organizer/Chair at ISMB 2013 Editorial Board of Scientific Reports (NPG) (since 06/13) Associate Editor BMC Bioinformatics (since 05/10) Academic Editor Microbial Cell (since 07/16)


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Funding Org.

Project Number


Funding Period

Max Planck Society

Project grant The human RNA-binding protein

interactome 78 2015

BMBF (DE)

01GS08171 NGFNplus 500 2008–2011

Max Planck Society

Max-Planck Research

Group Molecular Interaction Networks 2900 2007–2016

Publications [1] Apelt, L., Knockenhauer, K.E., Leksa, N.C., Benlasfer, N., Schwartz, T.U., and Stelzl, U.

Systematic Protein-Protein Interaction Analysis Reveals Intersubcomplex Contacts in the Nuclear Pore Complex. Mol Cell Proteomics 2016, 15, 2594-2606. 10.1074/mcp.M115.054627

[2] Bachmann, V.A., Mayrhofer, J.E., Ilouz, R., Tschaikner, P., Raffeiner, P., Rock, R., Courcelles, M., Apelt, F., Lu, T.-W., and Baillie, G.S., et al. Gpr161 anchoring of PKA consolidates GPCR and cAMP signaling. Proc Natl Acad Sci U S A 2016, 113, 7786-7791. 10.1073/pnas.1608061113

[3] Bohme, M.A., Beis, C., Reddy-Alla, S., Reynolds, E., Mampell, M.M., Grasskamp, A.T., Lutzkendorf, J., Bergeron, D.D., Driller, J.H., and Babikir, H., et al. Active zone scaffolds differentially accumulate Unc13 isoforms to tune Ca(2+) channel-vesicle coupling. Nat Neurosci 2016, 19, 1311-1320. 10.1038/nn.4364.

[4] Schutze, T., Ulrich, A.K.C., Apelt, L., Will, C.L., Bartlick, N., Seeger, M., Weber, G., Luhrmann, R., Stelzl, U., and Wahl, M.C. Multiple protein-protein interactions converging on the Prp38 protein during activation of the human spliceosome. RNA 2016, 22, 265-277. 10.1261/rna.054296.115

[5] Weir, M.E., Mann, J.E., Corwin, T., Fulton, Z.W., Hao, J.M., Maniscalco, J.F., Kenney, M.C., Roman Roque, K.M., Chapdelaine, E.F., and Stelzl, U., et al. Novel autophosphorylation sites of Src family kinases regulate kinase activity and SH2 domain-binding capacity. FEBS Lett 2016, 590, 1042-1052. 10.1002/1873-3468.12144

[6] Absmeier, E., Rosenberger, L., Apelt, L., Becke, C., Santos, K.F., Stelzl, U., and Wahl, M.C. A noncanonical PWI domain in the N-terminal helicase-associated region of the spliceosomal Brr2 protein. Acta Crystallogr D Biol Crystallogr 2015, 71, 762-771. 10.1107/S1399004715001005

[7] Grossmann, A., Benlasfer, N., Birth, P., Hegele, A., Wachsmuth, F., Apelt, L., and Stelzl, U. Phospho-tyrosine dependent protein-protein interaction network. Mol Syst Biol 2015, 11, 794. 10.15252/msb.20145968

[8] Stelzl, U. E. coli network upgrade. Nat Biotechnol 2014, 32, 241-243. 10.1038/nbt.2848 [9] Woodsmith, J., and Stelzl, U. Studying post-translational modifications with protein

interaction networks. Curr Opin Struct Biol 2014, 24, 34-44. 10.1016/j.sbi.2013.11.009 [10] Kamburov, A., Stelzl, U., Lehrach, H., and Herwig, R. The ConsensusPathDB interaction

database: 2013 update. Nucleic Acids Res 2013, 41, D793-800. 10.1093/nar/gks1055 [11] Stelzl, U. Molecular interaction networks in the analyses of sequence variation and

proteomics data. Proteomics Clin Appl 2013, 7, 727-732. 10.1002/prca.201300039 [12] Weimann, M., Grossmann, A., Woodsmith, J., Ozkan, Z., Birth, P., Meierhofer, D.,

Benlasfer, N., Valovka, T., Timmermann, B., and Wanker, E.E., et al. A Y2H-seq approach defines the human protein methyltransferase interactome. Nat Methods 2013, 10, 339-342. 10.1038/nmeth.2397

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[13] Woodsmith, J., Kamburov, A., and Stelzl, U. Dual coordination of post translational modifications in human protein networks. PLoS Comput Biol 2013, 9, e1002933. 10.1371/journal.pcbi.1002933

[14] Chua, J.J.E., Butkevich, E., Worseck, J.M., Kittelmann, M., Gronborg, M., Behrmann, E., Stelzl, U., Pavlos, N.J., Lalowski, M.M., and Eimer, S., et al. Phosphorylation-regulated axonal dependent transport of syntaxin 1 is mediated by a Kinesin-1 adapter. Proc Natl Acad Sci U S A 2012, 109, 5862-5867. 10.1073/pnas.1113819109

[15] Hegele, A., Kamburov, A., Grossmann, A., Sourlis, C., Wowro, S., Weimann, M., Will, C.L., Pena, V., Luhrmann, R., and Stelzl, U. Dynamic protein-protein interaction wiring of the human spliceosome. Mol Cell 2012, 45, 567-580. 10.1016/j.molcel.2011.12.034

[16] Hosur, R., Peng, J., Vinayagam, A., Stelzl, U., Xu, J., Perrimon, N., Bienkowska, J., and Berger, B. A computational framework for boosting confidence in high-throughput protein-protein interaction datasets. Genome Biol 2012, 13, R76. 10.1186/gb-2012-13-8-r76

[17] Kamburov, A., Grossmann, A., Herwig, R., and Stelzl, U. Cluster-based assessment of protein-protein interaction confidence. BMC Bioinformatics 2012a, 13, 262. 10.1186/1471-2105-13-262

[18] Kamburov, A., Stelzl, U., and Herwig, R. IntScore: a web tool for confidence scoring of biological interactions. Nucleic Acids Res 2012b, 40, W140-6. 10.1093/nar/gks492

[19] Worseck, J.M., Grossmann, A., Weimann, M., Hegele, A., and Stelzl, U. A stringent yeast two-hybrid matrix screening approach for protein-protein interaction discovery. Methods Mol Biol 2012, 812, 63-87. 10.1007/978-1-61779-455-1_4

[20] Elefsinioti, A., Sarac, O.S., Hegele, A., Plake, C., Hubner, N.C., Poser, I., Sarov, M., Hyman, A., Mann, M., and Schroeder, M., et al. Large-scale de novo prediction of physical protein-protein association. Mol Cell Proteomics 2011, 10, M111.010629. 10.1074/mcp.M111.010629

[21] Soler-Lopez, M., Zanzoni, A., Lluis, R., Stelzl, U., and Aloy, P. Interactome mapping suggests new mechanistic details underlying Alzheimer's disease. Genome Res 2011, 21, 364-376. 10.1101/gr.114280.110

[22] Vinayagam, A., Stelzl, U., Foulle, R., Plassmann, S., Zenkner, M., Timm, J., Assmus, H.E., Andrade-Navarro, M.A., and Wanker, E.E. A directed protein interaction network for investigating intracellular signal transduction. Sci Signal 2011, 4, rs8. 10.1126/scisignal.2001699

[23] Sylvester, M., Kliche, S., Lange, S., Geithner, S., Klemm, C., Schlosser, A., Grossmann, A., Stelzl, U., Schraven, B., and Krause, E., et al. Adhesion and degranulation promoting adapter protein (ADAP) is a central hub for phosphotyrosine-mediated interactions in T cells. PLoS One 2010, 5, e11708. 10.1371/journal.pone.0011708

[24] Vinayagam, A., Stelzl, U., and Wanker, E.E. Repeated two-hybrid screening detects transient protein–protein interactions. Theor Chem Acc 2010, 125, 613-619. 10.1007/s00214-009-0651-8

[25] Palidwor, G.A., Shcherbinin, S., Huska, M.R., Rasko, T., Stelzl, U., Arumughan, A., Foulle, R., Porras, P., Sanchez-Pulido, L., and Wanker, E.E., et al. Detection of alpha-rod protein repeats using a neural network and application to huntingtin. PLoS Comput Biol 2009, 5, e1000304. 10.1371/journal.pcbi.1000304

[26] Venkatesan, K., Rual, J.-F., Vazquez, A., Stelzl, U., Lemmens, I., Hirozane-Kishikawa, T., Hao, T., Zenkner, M., Xin, X., and Goh, K.-I., et al. An empirical framework for binary interactome mapping. Nat Methods 2009, 6, 83-90. 10.1038/nmeth.1280

[27] Ozlem Tastan Bishop, A., Stelzl, U., Pech, M., and Nierhaus, K.H. Characterization of RNA-protein interactions by phosphorothioate footprinting and its applications to the ribosome. Methods Mol Biol 2008, 488, 129-151. 10.1007/978-1-60327-475-3_9

[28] Stelzl, U., and Nierhaus, K.H. In vitro selection of random RNA fragments to identify protein-binding sites within large RNAs. Methods Mol Biol 2008, 488, 247-255. 10.1007/978-1-60327-475-3_17

[29] Grelle, G., Kostka, S., Otto, A., Kersten, B., Genser, K.F., Muller, E.-C., Walter, S., Boddrich, A., Stelzl, U., and Hanig, C., et al. Identification of VCP/p97, carboxyl terminus of Hsp70-interacting protein (CHIP), and amphiphysin II interaction partners using

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membrane-based human proteome arrays. Mol Cell Proteomics 2006, 5, 234-244. 10.1074/mcp.M500198-MCP200

[30] Horn, S.C., Lalowski, M., Goehler, H., Droge, A., Wanker, E.E., and Stelzl, U. Huntingtin interacts with the receptor sorting family protein GASP2. J Neural Transm 2006, 113, 1081-1090. 10.1007/s00702-006-0514-6

[31] Stelzl, U., and Wanker, E.E. The value of high quality protein-protein interaction networks for systems biology. Curr Opin Chem Biol 2006, 10, 551-558. 10.1016/j.cbpa.2006.10.005

[32] Weidemann, W., Stelzl, U., Lisewski, U., Bork, K., Wanker, E.E., Hinderlich, S., and Horstkorte, R. The collapsin response mediator protein 1 (CRMP-1) and the promyelocytic leukemia zinc finger protein (PLZF) bind to UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (GNE), the key enzyme of sialic acid biosynthesis. FEBS Lett 2006, 580, 6649-6654. 10.1016/j.febslet.2006.11.015

[33] Stelzl, U., Worm, U., Lalowski, M., Haenig, C., Brembeck, F.H., Goehler, H., Stroedicke, M., Zenkner, M., Schoenherr, A., and Koeppen, S., et al. A human protein-protein interaction network: a resource for annotating the proteome. Cell 2005, 122, 957-968. 10.1016/j.cell.2005.08.029

[34] Blume, A., Weidemann, W., Stelzl, U., Wanker, E.E., Lucka, L., Donner, P., Reutter, W., Horstkorte, R., and Hinderlich, S. Domain-specific characteristics of the bifunctional key enzyme of sialic acid biosynthesis, UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase. Biochem J 2004, 384, 599-607. 10.1042/BJ20040917

[35] Goehler, H., Lalowski, M., Stelzl, U., Waelter, S., Stroedicke, M., Worm, U., Droege, A., Lindenberg, K.S., Knoblich, M., and Haenig, C., et al. A protein interaction network links GIT1, an enhancer of huntingtin aggregation, to Huntington's disease. Mol Cell 2004, 15, 853-865. 10.1016/j.molcel.2004.09.016

[36] Stelzl, U., Zengel, J.M., Tovbina, M., Walker, M., Nierhaus, K.H., Lindahl, L., and Patel, D.J. RNA-structural mimicry in Escherichia coli ribosomal protein L4-dependent regulation of the S10 operon. J Biol Chem 2003, 278, 28237-28245. 10.1074/jbc.M302651200

[37] Connell, S.R., Trieber, C.A., Stelzl, U., Einfeldt, E., Taylor, D.E., and Nierhaus, K.H. The tetracycline resistance protein Tet(○) perturbs the conformation of the ribosomal decoding centre. Molecular Microbiology 2002, 45, 1463-1472. 10.1046/j.1365-2958.2002.03115.x

[38] Wilson, D.N., Blaha, G., Connell, S.R., Ivanov, P.V., Jenke, H., Stelzl, U., Teraoka, Y., and Nierhaus, K.H. Protein Synthesis at Atomic Resolution. Mechanistics of Translation in the Light of Highly Resolved Structures for the Ribosome. Curr Protein Pept Sci 2002, 3, 1-53. 10.2174/1389203023380846

[39] Stelzl, U., and Nierhaus, K.H. SERF: in vitro election of random RNA fragments to identify protein binding sites within large RNAs. Methods 2001a, 25, 351-357. 10.1006/meth.2001.1247

[40] Stelzl, U., and Nierhaus, K.H. A short fragment of 23S rRNA containing the binding sites for two ribosomal proteins, L24 and L4, is a key element for rRNA folding during early assembly. RNA 2001b, 7, 598-609. 10.1017/S1355838201002059

[41] Diedrich, G., Spahn, C.M., Stelzl, U., Schafer, M.A., Wooten, T., Bochkariov, D.E., Cooperman, B.S., Traut, R.R., and Nierhaus, K.H. Ribosomal protein L2 is involved in the association of the ribosomal subunits, tRNA binding to A and P sites and peptidyl transfer. EMBO J 2000, 19, 5241-5250. 10.1093/emboj/19.19.5241

[42] Stelzl, U., Spahn, C.M., and Nierhaus, K.H. Selecting rRNA binding sites for the ribosomal proteins L4 and L6 from randomly fragmented rRNA: application of a method called SERF. Proc Natl Acad Sci U S A 2000, 97, 4597-4602. 10.1073/pnas.090009297

[43] Blaha, G., Stelzl, U., Spahn, C.M., Agrawal, R.K., Frank, J., and Nierhaus, K.H. Preparation of functional ribosomal complexes and effect of buffer conditions on tRNA positions observed by cryoelectron microscopy. Meth Enzymol 317, 292-309. 10.1016/S0076-6879(00)17021-1

[44] Stelzl, U., Spahn, C.M., and Nierhaus, K.H. RNA-protein interactions in ribosomes: In vitro selection from randomly fragmented rRNA. Meth Enzymol 318, 251-268. 10.1016/S0076-6879(00)18057-7

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Patents

Wanker E.E., H. Göhler, M. Lalowski, U. Stelzl, H. Lehrach, M. Strödicke (2003). Disease Related Protein Network. EP03013957.0., PCT 2004/006617.

Wanker E.E., U. Stelzl, M. Lalowski, H. Göhler, U. Worm, C. Hänig, M. Strödicke (2005). Method for the identification of protein-protein interactions in a disease relevant protein network. EP04030994.0.

Selected oral presentations selected from >60 2016 BioMed X Lunch Talk Series / DKFZ Postdoc Network; 22nd Sept 2016; BioMed X

Innovation Center / DKFZ, Heidelberg, Germany; 2015 CSH Systems Biology: Networks, March 17 - 21, 2015, Cold Spring Harbor Laboratory,

New York, USA 2014 HUPO 2014, 13th Human Proteome Organization World Congress, October 5-8, 2014,

Madrid, Spain 2013 ESF-EMBO Symposium, Molecular Perspectives On Protein-Protein Interactions, 25-

30 May 2013, Pultusk, Poland 2012 Ian Lawson Van Toch Memorial Lecture, OCI Seminar in Computational Biology, Sept

27th 2012, Ontario Cancer Institute / University of Toronto, CA

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Curriculum Vitae – Ao. Univ.Prof. Dr. rer. nat. Klaus Zangger Institute of Chemistry, University of Graz, Heinrichstrasse 28, Ground floor, 8010 Graz, Austria, Phone: +43 (0) 316 380 8673, E-Mail: [email protected] Year/Place of Birth *1970/ Bruck an der Mur, Austria Nationality Austrian Orcid ID http://orcid.org/ 0000-0003-1682-1594 No of Publications 96 (52 first and/or corresponding) h-Index 26 (ISI Web of Knowledge, Nov, 2016) Supervised Ph.D. Students since 2007: 10 finished, 4 ongoing Key words NMR spectroscopy, peptide and protein structure, pure shift NMR, lipid

transporter Professional experiences

since 2005 Head of NMR Lab, Institute of Chemistry, University of Graz, Austria since 2004 Ao. University Professor, Institute of Chemistry, University of Graz,

Austria 2003 Habilitation in Chemistry and appointment as “Dozent” 2001-2003 Assistant professor, Institute of Chemistry, University of Graz, Austria 2000-2001 APART Fellowship at the Institute of Chemistry at the University of Graz 1999-2000 Post-doc at the Institute of Pharmaceutical Chemistry at the University

of Graz, Austria 1997-1999 Post-doc at the Institute of Biochemistry, Molecular Biology and

Biophysics at the University of Minnesota at Minneapolis, USA (Prof. Ian M. Armitage), (E. Schrödinger fellowship)

1993-1996 Assistant at the Institute of Organic Chemistry at the University of Graz Education 1993-1996 PhD thesis (with distinction) University of Graz, Austria, Supervisor:

Prof. Dr. H. Sterk 1988-1996 Study in Chemistry (with distinction), University of Graz, Austria Honors ▪ Awards 2000-2001 APART Fellowship (Austrian Academy of Sciences) 1997-1999 Erwin Schrödinger Fellowship (Austrian Science Funds FWF) Career related activities Organizer of the biannual NMR Summerschool Niederöblarn Organization of several workshops/symposia, like the CEUM 2010,

Austrian BioNMR Meeting 2016 Reviewer for several journals (e.g. JACS, PNAS, Angew.Chem, JBC)

~50 reviews per year Reviewer for several granting agencies (e.g. DFG (Germany), ANR

(France), ETH Zurich, EU projects. Member in various habilitation and professor hiring commissions,

University of Graz, Austria


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Funding Org.

Project Number


Funding Period

FWF P27793 Spatially-selective NMR

spectroscopy 273 2015-2017


W9

DK Molecular Enzymology 322 2014-2017

FWF P25880 NMR of paramagnetic relaxation

enhancements 231 2013-2016

FWF P24742 Pure shift NMR 276 2012-2015



Austrian Ministry of Science

University Infrastructure

700 MHz liquid state-NMR spectrometer

1700 2011

FWF P22630 NMR studies on the relaxosome 272 2010-2013



FWF P20020 Membrane-bound toxin-antitoxin

systems 271 2007-2011

Publications [1] Reichart, B., Guedes de la Cruz, G., Zangger, K., Kappe, C.O., Glasnov, T.

Copper/Nafion-Catalyzed Hydroarylation Process Involving Ketenimine Intermediates: A Novel and Synthetic Approach to 4-Sulfonamidoquinoline-2-ones and Derivatives Thereof Advanced Synthesis & Catalysis 2016, 358 (1), 50–55. 10.1002/adsc.201500942

[2] Nusshold, C., Ullen, A., Kogelnik, N., Bernhart, E., Reicher, H., Plastira, I., Glasnov, T., Zangger, K., Rechberger, G., Kollroser, M., Fauler, G., Wolinski, H., Weksler, B.B., Romero, I.A., Kohlwein, S.D., Couraud, P.-O., Malle, E., Sattler, W. Assessment of electrophile damage in a human brain endothelial cell line utilizing a clickable alkyne analog of 2-chlorohexadecanal Free radical biology & medicine 2016, 90, 59–74. 10.1016/j.freeradbiomed.2015.11.010

[3] Guttenberger, N., Glabonjat, R.A., Jensen, K.B., Zangger, K., Francesconi, K.A. Synthesis of two arsenic-containing cyclic ethers: Model compounds for a novel group of naturally-occurring arsenolipids Tetrahedron Letters 2016, 57 (41), 4578–4580. 10.1016/j.tetlet.2016.08.097

[4] Glanzer, S., Kunert, O., Zangger, K. Determination of unresolved heteronuclear scalar coupling constants by J(up)-HSQMBC Journal of magnetic resonance (San Diego, Calif. : 1997) 2016, 268, 88–94. 10.1016/j.jmr.2016.05.002

[5] Fercher, C., Probst, I., Kohler, V., Goessweiner-Mohr, N., Arends, K., Grohmann, E., Zangger, K., Meyer, N.H., Keller, W. VirB8-like protein TraH is crucial for DNA transfer in Enterococcus faecalis Scientific reports 2016, 6, 24643. 10.1038/srep24643

[6] Fercher, C., Keller, W., Zangger, K., Meyer, N.H. 1H, 15N and 13C chemical shift assignment of the Gram-positive conjugative transfer protein TraHpIP501 Biomol NMR Assign 2016, 10 (1), 163–166. 10.1007/s12104-015-9658-3

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[7] Zorzini, V., Buts, L., Schrank, E., Sterckx, Y.G.J., Respondek, M., Engelberg-Kulka, H., Loris, R., Zangger, K., van Nuland, N.A.J. Escherichia coli antitoxin MazE as transcription factor: insights into MazE-DNA binding Nucleic acids research 2015, 43 (2), 1241–1256. 10.1093/nar/gku1352

[8] Zangger, K. Pure shift NMR Progress in Nuclear Magnetic Resonance Spectroscopy 2015, 86-87C, 1–20. 10.1016/j.pnmrs.2015.02.002

[9] Ullen, A., Nusshold, C., Glasnov, T., Saf, R., Cantillo, D., Eibinger, G., Reicher, H., Fauler, G., Bernhart, E., Hallstrom, S., Kogelnik, N., Zangger, K., Oliver Kappe, C., Malle, E., Sattler, W. Covalent adduct formation between the plasmalogen-derived modification product 2-chlorohexadecanal and phloretin Biochemical pharmacology 2015, 93 (4), 470–481. 10.1016/j.bcp.2014.12.017

[10] Roier, S., Blume, T., Klug, L., Wagner, G.E., Elhenawy, W., Zangger, K., Prassl, R., Reidl, J., Daum, G., Feldman, M.F., Schild, S. A basis for vaccine development: Comparative characterization of Haemophilus influenzae outer membrane vesicles International journal of medical microbiology : IJMM 2015, 305 (3), 298–309. 10.1016/j.ijmm.2014.12.005

[11] Mauhart, J., Glanzer, S., Sakhaii, P., Bermel, W., Zangger, K. Faster and cleaner real-time pure shift NMR experiments Journal of magnetic resonance (San Diego, Calif. : 1997) 2015, 259, 207–215. 10.1016/j.jmr.2015.08.011

[12] Glanzer, S., Zangger, K. Uniform Reduction of Scalar Coupling by Real-Time Homonuclear J-Downscaled NMR Chemphyschem : a European journal of chemical physics and physical chemistry 2015, 16 (15), 3313–3317. 10.1002/cphc.201500640

[13] Glanzer, S., Zangger, K. Visualizing unresolved scalar couplings by real-time J-upscaled NMR Journal of the American Chemical Society 2015, 137 (15), 5163–5169. 10.1021/jacs.5b01687

[14] Glanzer, S., Pulido, S.A., Tutz, S., Wagner, G.E., Kriechbaum, M., Gubensak, N., Trifunovic, J., Dorn, M., Fabian, W.M.F., Novak, P., Reidl, J., Zangger, K. Structural and functional implications of the interaction between macrolide antibiotics and bile acids Chemistry (Weinheim an der Bergstrasse, Germany) 2015, 21 (11), 4350–4358. 10.1002/chem.201406413

[15] Dupe, A., Judmaier, M.E., Belaj, F., Zangger, K., Mosch-Zanetti, N.C. Activation of molecular oxygen by a molybdenum complex for catalytic oxidation Dalton transactions (Cambridge, England : 2003) 2015, 44 (47), 20514–20522. 10.1039/c5dt02931g

[16] Boeszoermenyi, A., Nagy, H.M., Arthanari, H., Pillip, C.J., Lindermuth, H., Luna, R.E., Wagner, G., Zechner, R., Zangger, K., Oberer, M. Structure of a CGI-58 motif provides the molecular basis of lipid droplet anchoring J Biol Chem 2015, 290 (44), 26361–26372. 10.1074/jbc.M115.682203

[17] Amanpour, T., Zangger, K., Belaj, F., Bazgir, A., Dallinger, D., Kappe, C.O. A detailed investigation of the multicomponent reaction of salicylaldehyde, ethyl acetoacetate and isocyanides under microwave heating Tetrahedron 2015, 71 (39), 7159–7169. 10.1016/j.tet.2014.12.002

[18] Wagner, G.E., Gutfreund, S., Fauland, K., Keller, W., Valenta, R., Zangger, K. Backbone resonance assignment of Alt a 1, a unique beta-barrel protein and the major allergen of Alternaria alternata Biomol NMR Assign 2014, 8 (2), 229–231. 10.1007/s12104-013-9489-z

[19] Vogel, A., Schmiedel, R., Hofmann, U., Gruber, K., Zangger, K. Converting Aspartase into a β-Amino Acid Lyase by Cluster Screening ChemCatChem 2014, 6 (4), 965–968. 10.1002/cctc.201300986

[20] Reinsperger, T., Luy, B. Homonuclear BIRD-decoupled spectra for measuring one-bond couplings with highest resolution: CLIP/CLAP-RESET and constant-time-CLIP/CLAP-RESET Journal of magnetic resonance (San Diego, Calif. : 1997) 2014, 239, 110–120. 10.1016/j.jmr.2013.11.015

[21] Pratter, S.M., Ivkovic, J., Birner-Gruenberger, R., Breinbauer, R., Zangger, K., Straganz, G.D. More than just a halogenase: modification of fatty acyl moieties by a trifunctional metal enzyme Chembiochem 2014, 15 (4), 567–574. 10.1002/cbic.201300345

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[22] Meyer, N.H., Zangger, K. Viva la Resolucion. Enhancing the Resolution of H-1 NMR Spectra by Broadband Homonuclear Decoupling Synlett 2014, 25 (7), 920–927. 10.1055/s-0033-1340635

[23] Meyer, N.H., Zangger, K. Boosting the resolution of 1H NMR spectra by homonuclear broadband decoupling Chemphyschem : a European journal of chemical physics and physical chemistry 2014, 15 (1), 49–55. 10.1002/cphc.201300861

[24] Meyer, N.H., Zangger, K. Enhancing the resolution of multi-dimensional heteronuclear NMR spectra of intrinsically disordered proteins by homonuclear broadband decoupling Chemical communications (Cambridge, England) 2014, 50 (12), 1488–1490. 10.1039/c3cc48135b

[25] Lienhart, W.-D., Gudipati, V., Uhl, M.K., Binter, A., Pulido, S.A., Saf, R., Zangger, K., Gruber, K., Macheroux, P. Collapse of the native structure caused by a single amino acid exchange in human NAD(P)H:quinone oxidoreductase(1.) The FEBS journal 2014, 281 (20), 4691–4704. 10.1111/febs.12975

[26] Hlina, J., Arp, H., Walewska, M., Florke, U., Zangger, K., Marschner, C., Baumgartner, J. Coordination Chemistry of Cyclic Disilylated Germylenes and Stannylenes with Group 11 Metals Organometallics 2014, 33 (24), 7069–7077. 10.1021/om500668r

[27] Gubensäk, N., Fabian, W.M.F., Zangger, K. Disentangling scalar coupling patterns by real-time SERF NMR Chemical communications (Cambridge, England) 2014, 50 (82), 12254–12257. 10.1039/c4cc05892e

[28] Glanzer, S., Zangger, K. Directly decoupled diffusion-ordered NMR spectroscopy for the analysis of compound mixtures Chemistry (Weinheim an der Bergstrasse, Germany) 2014, 20 (35), 11171–11175. 10.1002/chem.201402920

[29] Dall'antonia, F., Pavkov-Keller, T., Zangger, K., Keller, W. Structure of allergens and structure based epitope predictions Methods (San Diego, Calif.) 2014, 66 (1), 3–21. 10.1016/j.ymeth.2013.07.024

[30] Wuensch, C., Lechner, H., Glueck, S.M., Zangger, K., Hall, M., Faber, K. Asymmetric Biocatalytic Cannizzaro-Type Reaction ChemCatChem 2013, 5 (7), 1744–1748. 10.1002/cctc.201300028

[31] Wagner, G.E., Sakhaii, P., Bermel, W., Zangger, K. Monitoring fast reactions by spatially-selective and frequency-shifted continuous NMR spectroscopy: application to rapid-injection protein unfolding Chem Commun 2013, 49 (30), 3155–3157. 10.1039/c3cc39107h

[32] Schrank, E., Wagner, G.E., Zangger, K. Solution NMR studies on the orientation of membrane-bound peptides and proteins by paramagnetic probes Molecules (Basel, Switzerland) 2013, 18 (7), 7407–7435. 10.3390/molecules18077407

[33] Sakhaii, P., Haase, B., Bermel, W., Kerssebaum, R., Wagner, G.E., Zangger, K. Broadband homodecoupled NMR spectroscopy with enhanced sensitivity J Magn Reson 2013, 233, 92–95. 10.1016/j.jmr.2013.05.008

[34] Redzej, A., Ilangovan, A., Lang, S., Gruber, C.J., Topf, M., Zangger, K., Zechner, E.L., Waksman, G. Structure of a translocation signal domain mediating conjugative transfer by type IV secretion systems Molecular microbiology 2013, 89 (2), 324–333. 10.1111/mmi.12275

[35] Praznik, W., Loppert, R., Cruz Rubio, J.M., Zangger, K., Huber, A. Structure of fructo-oligosaccharides from leaves and stem of Agave tequilana Weber, var. azul Carbohydrate research 2013, 381, 64–73. 10.1016/j.carres.2013.08.025

[36] Meyer, N.H., Zangger, K. Boosting the resolution of 1H NMR spectra by homonuclear broadband decoupling ChemPhysChem 2013, 15 (1), 49–55.

[37] Meyer, N.H., Zangger, K. Simplifying proton NMR spectra by instant homonuclear broadband decoupling Angew Chem Int Ed Engl 2013, 52 (28), 7143–7146. 10.1002/anie.201300129

[38] Hocking, H.G., Zangger, K., Madl, T. Studying the structure and dynamics of biomolecules by using soluble paramagnetic probes Chemphyschem : a European journal of chemical physics and physical chemistry 2013, 14 (13), 3082–3094. 10.1002/cphc.201300219

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[39] Glanzer, S., Schrank, E., Zangger, K. A general method for diagonal peak suppression in homonuclear correlated NMR spectra by spatially and frequency selective pulses J.Magn.Reson. 2013, 323, 1–6.

[40] Novak, P., Jednačak, T., Parlov Vuković, J., Zangger, K., Rubčić, M., Galić, N., Hrenar, T. Synthesis, Structural Characterization and Hydrogen Bonding of Mono(salicylidene)carbohydrazide Croatica Chemica Acta 2012, 85 (4), 451–456. 10.5562/cca2123

[41] Kosol, S., Schrank, E., Krajačić, M.B., Wagner, G.E., Meyer, N.H., Göbl, C., Rechberger, G.N., Zangger, K., Novak, P. Probing the interactions of macrolide antibiotics with membrane-mimetics by NMR spectroscopy Journal of medicinal chemistry 2012, 55 (11), 5632–5636. 10.1021/jm300647f

[42] Kosol, S., Schrank, E., Bukvic-Krajacic, M., Wagner, G.E., Meyer, H., Göbl, C., Zangger, K., Novak, P. Probing the Interactions of Macrolide Antibiotics with Membrane-Mimetics by NMR Spectroscopy J.Med.Chem. 2012, 55 (11), 5632–5636.

[43] Holzer, M., Zangger, K., El-Gamal, D., Binder, V., Curcic, S., Konya, V., Schuligoi, R., Heinemann, A., Marsche, G. Myeloperoxidase-derived chlorinating species induce protein carbamylation through decomposition of thiocyanate and urea: novel pathways generating dysfunctional high-density lipoprotein Antioxidants & redox signaling 2012, 17 (8), 1043–1052. 10.1089/ars.2011.4403

[44] Hohlweg, W., Kosol, S., Zangger, K. Determining the orientation and localization of membrane-bound peptides Curr Protein Pept Sci 2012, 13 (3), 267–279.

[45] Fröhlich, R.F., Schrank, E., Zangger, K. 2,2,2-Trifluoroethyl 6-thio-beta-D-glucopyranoside as a selective tag for cysteines in proteins Carbohydr Res 2012, 361, 100–104. 10.1016/j.carres.2012.08.010

[46] Falsone, S.F., Meyer, N.H., Schrank, E., Leitinger, G., Pham, C.L., Fodero-Tavoletti, M.T., Holmberg, M., Dulle, M., Scicluna, B., Gesslbauer, B., Ruckert, H.M., Wagner, G.E., Merle, D.A., Nollen, E.A., Kungl, A.J., Hill, A.F., Cappai, R., Zangger, K. SERF protein is a direct modifier of amyloid fiber assembly Cell Rep 2012, 2 (2), 358–371. 10.1016/j.celrep.2012.06.012

[47] Durchschein, K., Wallner, S., Macheroux, P., Zangger, K., Fabian, W.M.F., Faber, K. Unusual C=C bond isomerization of an alpha,beta-unsaturated gamma-butyrolactone catalysed by flavoproteins from the old yellow enzyme family Chembiochem 2012, 13 (16), 2346–2351. 10.1002/cbic.201200475

[48] Stueckler, C., Winkler, C.K., Hall, M., Hauer, B., Bonnekessel, M., Zangger, K., Faber, K. Stereo-Controlled Asymmetric Bioreduction of α,β-Dehydroamino Acid Derivatives Advanced Synthesis & Catalysis 2011, 353 (7), 1169–1173. 10.1002/adsc.201100042

[49] Spirk, S., Salentinig, S., Zangger, K., Belaj, F., Pietschnig, R. Self-assembly of tert -butyl silanetriol in solution and aggregation with tetrahydrofuran Supramolecular Chemistry 2011, 23 (12), 801–805. 10.1080/10610278.2011.622380

[50] Lang, S., Kirchberger, P.C., Gruber, C.J., Redzej, A., Raffl, S., Zellnig, G., Zangger, K., Zechner, E.L. An activation domain of plasmid R1 TraI protein delineates stages of gene transfer initiation Molecular microbiology 2011, 82 (5), 1071–1085. 10.1111/j.1365-2958.2011.07872.x

[51] Falsone, S.F., Leitinger, G., Karner, A., Kungl, A.J., Kosol, S., Cappai, R., Zangger, K. The neurotransmitter serotonin interrupts alpha-synuclein amyloid maturation Biochim Biophys Acta 2011, 1814 (5), 553–561. 10.1016/j.bbapap.2011.02.008

[52] Durchschein, K., Fabian, W.M.F., Macheroux, P., Zangger, K., Trimmel, G., Faber, K. Reductive biotransformation of nitroalkenes via nitroso-intermediates to oxazetes catalyzed by xenobiotic reductase A (XenA) Organic & biomolecular chemistry 2011, 9 (9), 3364–3369. 10.1039/c0ob01216e

[53] Legat, A., Gruber, C., Zangger, K., Wanner, G., Stan-Lotter, H. Identification of polyhydroxyalkanoates in Halococcus and other haloarchaeal species Applied microbiology and biotechnology 2010, 87 (3), 1119–1127. 10.1007/s00253-010-2611-6

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[54] Kosol, S., Zangger, K. Dynamics and orientation of a cationic antimicrobial peptide in two membrane-mimetic systems J Struct Biol 2010, 170 (1), 172–179. 10.1016/j.jsb.2009.12.026

[55] Jonge, N.d., Hohlweg, W., Garcia-Pino, A., Respondek, M., Buts, L., Haesaerts, S., Lah, J., Zangger, K., Loris, R. Structural and thermodynamic characterization of Vibrio fischeri CcdB J Biol Chem 2010, 285 (8), 5606–5613. 10.1074/jbc.M109.068429

[56] Grossauer, J., Kosol, S., Schrank, E., Zangger, K. The peptide hormone ghrelin binds to membrane-mimetics via its octanoyl chain and an adjacent phenylalanine Bioorg Med Chem 2010, 18 (15), 5483–5488. 10.1016/j.bmc.2010.06.062

[57] Göbl, C., Kosol, S., Stockner, T., Rückert, H.M., Zangger, K. Solution structure and membrane binding of the toxin fst of the par addiction module Biochemistry 2010, 49 (31), 6567–6575. 10.1021/bi1005128

[58] Göbl, C., Dulle, M., Hohlweg, W., Grossauer, J., Falsone, S.F., Glatter, O., Zangger, K. Influence of phosphocholine alkyl chain length on peptide-micelle interactions and micellar size and shape J Phys Chem B 2010, 114 (13), 4717–4724. 10.1021/jp9114089

[59] Zangger, K., Respondek, M., Gobl, C., Hohlweg, W., Rasmussen, K., Grampp, G., Madl, T. Positioning of micelle-bound peptides by paramagnetic relaxation enhancements J Phys Chem B 2009, 113 (13), 4400–4406. 10.1021/jp808501x

[60] Respondek, M., Buts, L., Jonge, N.d., Haesaerts, S., Loris, R., van Melderen, L., Wyns, L., Zangger, K. Sequence-specific 1H, 15N and 13C resonance assignments of the 23.7-kDa homodimeric toxin CcdB from Vibrio fischeri Biomol NMR Assign 2009, 3 (1), 145–147. 10.1007/s12104-009-9161-9

[61] Madl, T., Bermel, W., Zangger, K. Use of relaxation enhancements in a paramagnetic environment for the structure determination of proteins using NMR spectroscopy Angew Chem Int Ed Engl 2009, 48 (44), 8259–8262. 10.1002/anie.200902561

[62] Jonge, N.d., Garcia-Pino, A., Buts, L., Haesaerts, S., Charlier, D., Zangger, K., Wyns, L., Greve, H.d., Loris, R. Rejuvenation of CcdB-poisoned gyrase by an intrinsically disordered protein domain Mol Cell 2009, 35 (2), 154–163. 10.1016/j.molcel.2009.05.025

[63] Falsone, S.F., Kungl, A.J., Rek, A., Cappai, R., Zangger, K. The molecular chaperone Hsp90 modulates intermediate steps of amyloid assembly of the Parkinson-related protein alpha-synuclein J Biol Chem 2009, 284 (45), 31190–31199. 10.1074/jbc.M109.057240

[64] Zangger, K., Gossler, R., Khatai, L., Lohner, K., Jilek, A. Structures of the glycine-rich diastereomeric peptides bombinin H2 and H4 Toxicon 2008, 52 (2), 246–254. 10.1016/j.toxicon.2008.05.011

[65] Respondek, M., Madl, T., Göbl, C., Golser, R., Zangger, K. Mapping the orientation of helices in micelle-bound peptides by paramagnetic relaxation waves Journal of the American Chemical Society 2007, 129 (16), 5228–5234. 10.1021/ja069004f

[66] Oberer, M., Zangger, K., Gruber, K., Keller, W. The solution structure of ParD, the antidote of the ParDE toxin antitoxin module, provides the structural basis for DNA and toxin binding Protein Sci 2007, 16 (8), 1676–1688. 10.1110/ps.062680707

[67] Stadlbauer, W., Täubl, A.E., Dang, H.V., Reidlinger, C., Zangger, K. Synthesis of quinolin-4-yl substituted malonates, cyanoacetates, acetoacetates and related compounds Journal of Heterocyclic Chemistry 2006, 43 (1), 117–125. 10.1002/jhet.5570430118

[68] Madl, T., van Melderen, L., Mine, N., Respondek, M., Oberer, M., Keller, W., Khatai, L., Zangger, K. Structural basis for nucleic acid and toxin recognition of the bacterial antitoxin CcdA J Mol Biol 2006, 364 (2), 170–185.

[69] Stranzl, G.R., Gruber, K., Steinkellner, G., Zangger, K., Schwab, H., Kratky, C. Observation of a short, strong hydrogen bond in the active site of hydroxynitrile lyase from Hevea brasiliensis explains a large pKa shift of the catalytic base induced by the reaction intermediate J Biol Chem 2004, 279 (5), 3699–3707. 10.1074/jbc.M306814200

[70] Messerschmidt, Handbook of Metallothioneins. John Wiley & Sons: Chichester; UK, 2004.

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[71] Khatai, L., Goessler, W., Lorencova, H., Zangger, K. Modulation of nitric oxide-mediated metal release from metallothionein by the redox state of glutathione in vitro Eur J Biochem 2004, 271 (12), 2408-2416.

[72] Cobine, P.A., McKay, R.T., Zangger, K., Dameron, C.T., Armitage, I.M. Solution structure of Cu6 metallothionein from the fungus Neurospora crassa Eur J Biochem 2004, 271 (21), 4213-4221.

[73] Zangger, K., Pervushin, K., Sterk, H., Lange, A.J., Okar, D.A. Sequence-specific (1)H, (13)C and (15)N resonance assignments of the rat liver fructose-2,6-bisphosphatase domain J Biomol NMR 2003, 27 (3), 281-282.

[74] Zangger, K., Oberer, M., Keller, W., Sterk, H. X-filtering for a range of coupling constants: application to the detection of intermolecular NOEs J Magn Reson 2003, 160 (2), 97-106.

[75] Kunert, O., Stingl, H., Rosian, E., Krssak, M., Bernroider, E., Seebacher, W., Zangger, K., Staehr, P., Chandramouli, V., Landau, B.R., Nowotny, P., Waldhausl, W., Haslinger, E., Roden, M. Measurement of fractional whole-body gluconeogenesis in humans from blood samples using 2H nuclear magnetic resonance spectroscopy Diabetes 2003, 52 (10), 2475–2482.

[76] Zangger, K., Armitage, I.M. Dynamics of interdomain and intermolecular interactions in mammalian metallothioneins J Inorg Biochem 2002, 88 (2), 135-143.

[77] Oberer, M., Zangger, K., Prytulla, S., Keller, W. The anti-toxin ParD of plasmid RK2 consists of two structurally distinct moieties and belongs to the ribbon-helix-helix family of DNA-binding proteins Biochem J 2002, 361 (Pt 1), 41-47.

[78] Dworczak, R., Fabian, W.M.F., Reidlinger, C., Rumpler, A., Schachner, J., Zangger, K. Nonlinear optical properties of diazabutadienes and -hexatrienes; experimental and computational aspects Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 2002, 58 (10), 2135–2144.

[79] Zangger, K., Shen, G., Oz, G., Otvos, J.D., Armitage, I.M. Oxidative dimerization in metallothionein is a result of intermolecular disulphide bonds between cysteines in the alpha-domain Biochem J 2001, 359 (Pt 2), 353-360.

[80] Zangger, K., Öz, G., Haslinger, E., Kunert, O., Armitage, I.M. Nitric oxide selectively releases metals from the amino-terminal domain of metallothioneins: potential role at inflammatory sites Faseb J 2001, 15 (7), 1303-1305.

[81] Zangger, K., Oberer, M., Sterk, H. Pure-phase selective excitation in fast-relaxing systems Journal of magnetic resonance (San Diego, Calif. : 1997) 2001, 152 (1), 48–56. 10.1006/jmre.2001.2401

[82] Stadler, A., Zangger, K., Belaj, F., Kollenz, G. Neat carbomethoxypivaloylketene—preparation and chemical reactivity Tetrahedron 2001, 57 (31), 6757–6763. 10.1016/s0040-4020(01)00625-1

[83] Öz, G., Zangger, K., Armitage, I.M. Three-dimensional structure and dynamics of a brain specific growth inhibitory factor: metallothionein-3 Biochemistry 2001, 40 (38), 11433-11441.

[84] Oettl, K., Greilberger, J., Zangger, K., Haslinger, E., Reibnegger, G., Jurgens, G. Radical-scavenging and iron-chelating properties of carvedilol, an antihypertensive drug with antioxidative activity Biochem Pharmacol 2001, 62 (2), 241–248.

[85] Zangger, K., Öz, G., Armitage, I.M. Re-evaluation of the binding of ATP to metallothionein J Biol Chem 2000, 275 (11), 7534–7538.

[86] Zangger, K., Armitage, I.M. The accordion-HMQC: heteronuclear correlations for a range of coupling constants Magn.Reson.Chem. 2000, 38, 452–458.

[87] Zangger, K., Öz, G., Otvos, J.D., Armitage, I.M. Three-dimensional solution structure of mouse [Cd7]-metallothionein-1 by homonuclear and heteronuclear NMR spectroscopy Protein Sci 1999, 8 (12), 2630–2638.

[88] Zangger, K., Armitage, L.M. Silver and gold NMR Metal-based drugs 1999, 6 (4-5), 239–245. 10.1155/mbd.1999.239

[89] Zangger, K., Armitage, I.M. Sensitivity-enhanced detection of fast exchanging protons by an exchange-edited gradient HEHAHA-HSQC experiment J Magn Reson 1998, 135 (1), 70–75.

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[90] Zangger, K., Sterk, H. Homonuclear broadband-decoupled NMR spectra J.Magn.Reson. 1997, 124, 486–489.

[91] Hickel, A., Gradnig, G., Schall, M., Zangger, K., Griengl, H. Determination of enantiomeric purity of mandelonitrile with derivatized cyclodextrins in water Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 1997, 53 (3), 451–455. 10.1016/s1386-1425(96)01838-0

[92] Zangger, K., Sterk, H. 1H-Detected 2D J-Resolved INEPT-INADEQUATE J.Magn.Reson.A 1996, 121, 56–59.

[93] Zangger, K., Sterk, H. Selective Measurement of Chemical Exchange Rates by Synchronous Nutation in Nuclear Magnetic Resonance Spectroscopy The Journal of Physical Chemistry 1996, 100 (26), 11198–11201. 10.1021/jp9604352

[94] Zangger, K., Sterk, H. Conformation of Peptide T in Aqueous Solution. Determination of Homonuclear Coupling Constants by Selective HOHAHA Techniques Magn.Reson.Chem. 1995, 33, 421–425.

[95] Zangger, K., Sterk, H. NMR Studies of Exchanging Amide Protons in 1-Desamino-8-D-vasopressin by Selective Hartmann-Hahn Transfer Mag.Reson.Chem. 1995, 33, 124–127.

[96] Zangger, K., Sterk, H. Pseudo-3D HOHAHA-ROESY with Doubly Selective Pulses. Application to a Decapeptide J.Magn.Reson.B 1995, 107, 186–188.

10 Selected recent oral presentations (all invited) 2016 “Using interrupted acquisition for resolution-enhanced real-time NMR” NMR

Minisymposium, Linz, Austria. June 2016 2016 “Enhancing the resolution of NMR spectra by interrupted acquisition” MatChemComp

Conference, Dubrovnik, Croatia. June 2016 2015 “Simplifying NMR spectra of drugs by pure shift experiments” NMR in Drug Discovery

workshop, Red Island, Croatia. September 2015 2015 “Enhancing the Resolution of Proton-detected NMR Spectra by Spatially-selective

Excitation” ENC Conference, Monterey, USA. April 2015 2015 “Enhancing the Resolution of Proton-detected NMR Spectra by Spatially-selective

Excitation” NMRS Conference, Amritsar, India. March 2015 2015 “Simplifying proton-detected NMR spectra by spatially-selective excitation.” MMCE

Conference, Krynica, Poland. February 2015 2014 “Simplifying proton-detected NMR spectra by spatially-selective excitation.” CEUM

Conference, Zagreb, Croatia. October 2014 2014 “Simplifying proton NMR spectra by spatially-selective pulses” SMASH NMR

Conference, Atlanta, Georgia, USA. September 2014 2014 “Simplifying proton-detected spectra by spatially and frequency-selective excitation”

Euromar Conference, Zurich, Switzerland. June 2014 2013 “Protein structure determination by liquid state NMR spectroscopy using paramagnetic

relaxation” Bio-Nano-Imaging Workshop, Vienna, Austria. May 2013

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Curriculum Vitae – Ao. Univ.-Prof. Dr. rer. nat. Ellen L. Zechner Institute of Molecular Biosciences, University of Graz, Humboldtstrasse 50, 1st floor, 8010 Graz, Austria, Phone: +43 (0) 316 380 5624, E-Mail: [email protected]

Year/Place of Birth *1961/ Florida, USA Nationality Austrian Orcid ID http://orcid.org/ 0000-0003-2035-1898 No of Publications 55 (31 first and/or corresponding) h-Index 20 (ISI Web of Knowledge, Nov, 2016) Supervised Ph.D. Students since 2007: 9 finished, 3 ongoing Key words bacterial communication, protein secretion, pathogenicity,

gastrointestinal pathogens, biofilms


since 2016 Vice President of the Austrian Science Fund (FWF), Biology and Medicine Section

2011-Sept 2016 Austrian Science Fund (FWF), Full Member of the Kuratorium Fields of Genetics, Microbiology and Biotechnology 2015 - 2016 Vice Dean of the Natural Sciences Faculty, University of Graz since 2001 Ao. University Professor, Institute of Molecular Biosciences, University of

Graz, 1999 - 2001 Assistant Professor, Institute of Molecular Biosciences, University of

Graz 1994 -1999 University Assistant, Institute of Microbiology, University of Graz 1992 - 1994 Postdoctoral fellow, Institute of Microbiology, University of Graz

Education 1986 -1991 Ph.D. in Molecular Biology, Sloan-Kettering Division, Cornell University,

Graduate School of Medical Sciences, New York, USA

1979-1983 B.A. degree in Chemistry and Biology, Oberlin College, Oberlin, USA

Honors ▪ Awards 2014/ 2015 Prizes awarded for Schneditz, G. et al. (2014) Enterotoxicity of a

nonribosomal peptide causes antibiotic-associated colitis. Proc Natl Acad Sci U S A 111: 13181-13186

(10,000 €) Top Abstract award, United European Gastroenterologists, Berlin and (4,000 €) Wewalka Prize, Salzburg

Career related activities since 2013 Advisory Board, Doktoratskolleg Molecular Fundamentals of

Inflammation (MOLIN), Medical University of Graz since 2016 NAWI-Graz Steering Committee, University of Graz / Technical

University of Graz cooperative research and teaching program since 2016 Supervisory Board, Doctoral Academy Graz 2009 - 2016 Spokesperson for the University of Graz core research area “Molecular

Enzymology and Physiology” 2007 - 2016 Director, Doctoral School of Molecular Biology and Biochemistry,

University of Graz 2006 - 2015 University of Graz representative, COIMBRA Universities’ Task Force

“Doctoral Studies and Research” 2006 - 2008 Treasurer, International Society for Plasmid Biology and other Mobile

Genetic Elements since 2005 Director of the FWF-financed Doktoratskolleg Molecular Enzymology,

University of Graz and Graz University of Technology, Austria Since 2004 Trustee, Brian M. Wilkins Research Fund

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Funding Org.

Project Number


Funding Period

BioTechMed/ University Support

-

Immunomodulatory and Antiproliferative Bioactivities of the Bacterial Secretome

in Gastrointestinal Disease and Therapy (Coordinator & PI)

138 starting in

2017

University Support

- Bacterial communication and

competition in the human intestine 193 2016-2019

BioTechMed-Graz

- Bacterial metabolite signal molecules in the intestinal

microbiota 155 2014-2017

FWF P24016 Regulation of the initiation of bacterial type IV secretion

326 2011-2016

EU EFRE / County of

Styria -

Bacterial type IV protein translocation and Catheter-

associated urinary tract infections 310 2011-2014

FWF P21434

Genetic analysis of bacterial biofilm formation and migration on

urethral catheters (co-PI: A. Reisner)

276 2009-2012

Publications

[1] Zechner, E.L. Inflammatory disease caused by intestinal pathobionts Curr Opin Microbiol 2017, COMICR-D-16-00096, in press.

[2] Ilangovan, A., Zanetti, G., Zechner, E.L., Waksman, G. Cryo-EM structure of a relaxase reveals the molecular basis of DNA unwinding during bacterial conjugation Cell 2017, CELL-D-16-02111, in revision.

[3] Kienesberger, S., Cox, L.M., Livanos, A., Zhang, X.S., Chung, J., Perez-Perez, G.I., Gorkiewicz, G., Zechner, E.L., Blaser, M.J. Gastric Helicobacter pylori Infection Affects Local and Distant Microbial Populations and Host Responses Cell Rep 2016, 14 (6), 1395-1407. 10.1016/j.celrep.2016.01.017

[4] Gruber, C.J., Lang, S., Rajendra, V.K., Nuk, M., Raffl, S., Schildbach, J.F., Zechner, E.L. Conjugative DNA Transfer Is Enhanced by Plasmid R1 Partitioning Proteins Front Mol Biosci 2016, 3, 32. 10.3389/fmolb.2016.00032

[5] Zollner-Schwetz, I., Herzog, K.A., Feierl, G., Leitner, E., Schneditz, G., Sprenger, H., Prattes, J., Petritsch, W., Wenzl, H., Kump, P., Gorkiewicz, G., Zechner, E., Hogenauer, C. The Toxin-Producing Pathobiont Klebsiella oxytoca Is Not Associated with Flares of Inflammatory Bowel Diseases Dig Dis Sci 2015. 10.1007/s10620-015-3765-y

[6] Schneditz, G., Rentner, J., Roier, S., Pletz, J., Herzog, K.A., Bucker, R., Troeger, H., Schild, S., Weber, H., Breinbauer, R., Gorkiewicz, G., Hogenauer, C., Zechner, E.L. Enterotoxicity of a nonribosomal peptide causes antibiotic-associated colitis Proc Natl Acad Sci U S A 2014, 111 (36), 13181-13186. 10.1073/pnas.1403274111

[7] Reisner, A., Maierl, M., Jorger, M., Krause, R., Berger, D., Haid, A., Tesic, D., Zechner, E.L. Type 1 fimbriae contribute to catheter-associated urinary tract infections caused by Escherichia coli J Bacteriol 2014, 196 (5), 931-939. 10.1128/JB.00985-13

[8] Lang, S., Gruber, C.J., Raffl, S., Reisner, A., Zechner, E.L. Common requirement for the relaxosome of plasmid R1 in multiple activities of the conjugative type IV secretion system J Bacteriol 2014, 196 (11), 2108-2121. 10.1128/JB.00045-13

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[9] Kienesberger, S., Sprenger, H., Wolfgruber, S., Halwachs, B., Thallinger, G.G., Perez-Perez, G.I., Blaser, M.J., Zechner, E.L., Gorkiewicz, G. Comparative genome analysis of Campylobacter fetus subspecies revealed horizontally acquired genetic elements important for virulence and niche specificity PLoS One 2014, 9 (1), e85491. 10.1371/journal.pone.0085491

[10] Herzog, K.A., Schneditz, G., Leitner, E., Feierl, G., Hoffmann, K.M., Zollner-Schwetz, I., Krause, R., Gorkiewicz, G., Zechner, E.L., Hogenauer, C. Genotypes of Klebsiella oxytoca isolates from patients with nosocomial pneumonia are distinct from those of isolates from patients with antibiotic-associated hemorrhagic colitis J Clin Microbiol 2014, 52 (5), 1607-1616. 10.1128/JCM.03373-13

[11] Gibert, M., Juarez, A., Zechner, E.L., Madrid, C., Balsalobre, C. TrhR, TrhY and HtdA, a novel regulatory circuit that modulates conjugation of the IncHI plasmids Mol Microbiol 2014, 94, 1146-1161. 10.1111/mmi.12823

[12] Redzej, A., Ilangovan, A., Lang, S., Gruber, C.J., Topf, M., Zangger, K., Zechner, E.L., Waksman, G. Structure of a translocation signal domain mediating conjugative transfer by type IV secretion systems Mol Microbiol 2013, 89 (2), 324-333. 10.1111/mmi.12275

[13] Alperi, A., Larrea, D., Fernandez-Gonzalez, E., Dehio, C., Zechner, E.L., Llosa, M. A translocation motif in relaxase TrwC specifically affects recruitment by its conjugative Type IV Secretion System J Bacteriol 2013, 195, 4999-5006. 10.1128/JB.00367-13

[14] Zechner, E.L., Lang, S., Schildbach, J.F. Assembly and mechanisms of bacterial type IV secretion machines Philos Trans R Soc Lond B Biol Sci 2012, 367 (1592), 1073-1087. 10.1098/rstb.2011.0207

[15] Sprenger, H., Zechner, E.L., Gorkiewicz, G. So close and yet so far - Molecular Microbiology of Campylobacter fetus subspecies Eur J Microbiol Immunol 2012, 2 (1), 66-75. 10.1556/EuJMI.2.2012.1.10

[16] Reisner, A., Wolinski, H., Zechner, E.L. In situ monitoring of IncF plasmid transfer on semi-solid agar surfaces reveals a limited invasion of plasmids in recipient colonies Plasmid 2012, 67 (2), 155-161. 10.1016/j.plasmid.2012.01.001

[17] Lang, S., Zechner, E.L. General requirements for protein secretion by the F-like conjugation system R1 Plasmid 2012, 67 (2), 128-138. 10.1016/j.plasmid.2011.12.014

[18] Nuk, M.R., Reisner, A., Zechner, E.L. The transfer operon of plasmid R1 extends beyond finO into the downstream replication genes Plasmid 2011, 65 (2), 150-158. 10.1016/j.plasmid.2010.12.003

[19] Nuk, M.R., Reisner, A., Neuwirth, M., Schilcher, K., Arnold, R., Jehl, A., Rattei, T., Zechner, E.L. Functional analysis of the finO distal region of plasmid R1 Plasmid 2011, 65 (2), 159-168. 10.1016/j.plasmid.2010.12.002

[20] Lang, S., Kirchberger, P.C., Gruber, C.J., Redzej, A., Raffl, S., Zellnig, G., Zangger, K., Zechner, E.L. An activation domain of plasmid R1 TraI protein delineates stages of gene transfer initiation Mol Microbiol 2011, 82 (5), 1071-1085. 10.1111/j.1365-2958.2011.07872.x

[21] Kienesberger, S., Schober Trummler, C., Fauster, A., Lang, S., Sprenger, H., Gorkiewicz, G., Zechner, E.L. Interbacterial macromolecular transfer by the Campylobacter fetus subsp. venerealis type IV secretion system J Bacteriol 2011, 193 (3), 744-758. 10.1128/JB.00798-10

[22] Kienesberger, S., Gorkiewicz, G., Wolinski, H., Zechner, E.L. New molecular microbiology approaches in the study of Campylobacter fetus Microb Biotechnol 2011, 4 (1), 8-19. 10.1111/j.1751-7915.2010.00173.x

[23] Lang, S., Gruber, K., Mihajlovic, S., Arnold, R., Gruber, C.J., Steinlechner, S., Jehl, M.A., Rattei, T., Frohlich, K.U., Zechner, E.L. Molecular recognition determinants for type IV secretion of diverse families of conjugative relaxases Mol Microbiol 2010, 78 (6), 1539-1555. 10.1111/j.1365-2958.2010.07423.x

[24] Joainig, M.M., Gorkiewicz, G., Leitner, E., Weberhofer, P., Zollner-Schwetz, I., Lippe, I., Feierl, G., Krause, R., Hinterleitner, T., Zechner, E.L., Hogenauer, C. Cytotoxic effects of Klebsiella oxytoca strains isolated from patients with antibiotic-associated hemorrhagic colitis or other diseases caused by infections and from healthy subjects J Clin Microbiol 2010, 48 (3), 817-824. 10.1128/JCM.01741-09

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[25] Hoffmann, K.M., Deutschmann, A., Weitzer, C., Joainig, M., Zechner, E., Hogenauer, C., Hauer, A.C. Antibiotic-associated hemorrhagic colitis caused by cytotoxin-producing Klebsiella oxytoca Pediatrics 2010, 125 (4), e960-963. 10.1542/peds.2009-1751

[26] Gorkiewicz, G., Kienesberger, S., Schober, C., Scheicher, S.R., Gully, C., Zechner, R., Zechner, E.L. A genomic island defines subspecies-specific virulence features of the host-adapted pathogen Campylobacter fetus subsp. venerealis J Bacteriol 2010, 192 (2), 502-517. 10.1128/JB.00803-09

[27] de la Cruz, F., Frost, L.S., Meyer, R.J., Zechner, E.L. Conjugative DNA metabolism in Gram-negative bacteria FEMS Microbiol Rev 2010, 34 (1), 18-40. 10.1111/j.1574-6976.2009.00195.x

[28] Sut, M.V., Mihajlovic, S., Lang, S., Gruber, C.J., Zechner, E.L. Protein and DNA effectors control the TraI conjugative helicase of plasmid R1 J Bacteriol 2009, 191 (22), 6888-6899. 10.1128/JB.00920-09

[29] Mihajlovic, S., Lang, S., Sut, M.V., Strohmaier, H., Gruber, C.J., Koraimann, G., Cabezon, E., Moncalian, G., de la Cruz, F., Zechner, E.L. Plasmid r1 conjugative DNA processing is regulated at the coupling protein interface J Bacteriol 2009, 191 (22), 6877-6887. 10.1128/JB.00918-09

[30] Kienesberger, S., Gorkiewicz, G., Joainig, M.M., Scheicher, S.R., Leitner, E., Zechner, E.L. Development of experimental genetic tools for Campylobacter fetus Appl Environ Microbiol 2007, 73 (14), 4619-4630.

[31] Reisner, A., Zechner, E.L., Lateral Mobility and Expression of Genes in Microbial Biofilms. In Understanding Biofilms and their importance in industry, environment, and medicine, Anne, J. a. S., W., Ed. Springer: 2006.

[32] Reisner, A., Krogfelt, K.A., Klein, B.M., Zechner, E.L., Molin, S. In vitro biofilm formation of commensal and pathogenic Escherichia coli strains: impact of environmental and genetic factors J Bacteriol 2006, 188 (10), 3572-3581.

[33] Reisner, A., Holler, B.M., Molin, S., Zechner, E.L. Synergistic effects in mixed Escherichia coli biofilms: conjugative plasmid transfer drives biofilm expansion J Bacteriol 2006, 188 (10), 3582-3588.

[34] Haft, R.J., Palacios, G., Nguyen, T., Mally, M., Gachelet, E.G., Zechner, E.L., Traxler, B. General mutagenesis of F plasmid TraI reveals its role in conjugative regulation J Bacteriol 2006, 188 (17), 6346-6353.

[35] Fernandez-Lopez, R., Machon, C., Longshaw, C.M., Martin, S., Molin, S., Zechner, E.L., Espinosa, M., Lanka, E., de la Cruz, F. Unsaturated fatty acids are inhibitors of bacterial conjugation Microbiology 2005, 151 (Pt 11), 3517-3526.

[36] Zechner, E.L., Bailey, M.J. The Horizontal Gene Pool: an ESF workshop summary Plasmid 2004, 51 (2), 67-74.

[37] Csitkovits, V.C., Dermic, D., Zechner, E.L. Concomitant reconstitution of TraI-catalyzed DNA transesterase and DNA helicase activity in vitro J Biol Chem 2004, 279 (44), 45477-45484.

[38] Reisner, A., Haagensen, J.A., Schembri, M.A., Zechner, E.L., Molin, S. Development and maturation of Escherichia coli K-12 biofilms Mol Microbiol 2003, 48 (4), 933-946.

[39] Gorkiewicz, G., Feierl, G., Schober, C., Dieber, F., Kofer, J., Zechner, R., Zechner, E.L. Species-specific identification of campylobacters by partial 16S rRNA gene sequencing J Clin Microbiol 2003, 41 (6), 2537-2546.

[40] Csitkovits, V.C., Zechner, E.L. Extent of single-stranded DNA required for efficient TraI helicase activity in vitro J Biol Chem 2003, 278 (49), 48696-48703.

[41] Schroder, G., Krause, S., Zechner, E.L., Traxler, B., Yeo, H.J., Lurz, R., Waksman, G., Lanka, E. TraG-like proteins of DNA transfer systems and of the Helicobacter pylori type IV secretion system: inner membrane gate for exported substrates? J Bacteriol 2002, 184 (10), 2767-2779.

[42] Reisner, A., Molin, S., Zechner, E.L. Recombinogenic engineering of conjugative plasmids with fluorescent marker cassettes FEMS Microbiol. Ecol. 2002, 42, 251-259.

[43] Gorkiewicz, G., Feierl, G., Zechner, R., Zechner, E.L. Transmission of Campylobacter hyointestinalis from a pig to a human J Clin Microbiol 2002, 40 (7), 2601-2605.

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[44] Karl, W., Bamberger, M., Zechner, E.L. Transfer protein TraY of plasmid R1 stimulates TraI-catalyzed oriT cleavage in vivo J Bacteriol 2001, 183 (3), 909-914. 10.1128/JB.183.3.909-914.2001

[45] Strohmaier, H., Noiges, R., Kotschan, S., Sawers, G., Hogenauer, G., Zechner, E.L., Koraimann, G. Signal transduction and bacterial conjugation: characterization of the role of ArcA in regulating conjugative transfer of the resistance plasmid R1 J Mol Biol 1998, 277 (2), 309-316. 10.1006/jmbi.1997.1598

[46] Kupelwieser, G., Schwab, M., Hogenauer, G., Koraimann, G., Zechner, E.L. Transfer protein TraM stimulates TraI-catalyzed cleavage of the transfer origin of plasmid R1 in vivo J Mol Biol 1998, 275 (1), 81-94. S0022-2836(97)91436-4 [pii]

10.1006/jmbi.1997.1436 [47] Grohmann, E., Moscoso, M., Zechner, E.L., del Solar, G., Espinosa, M. In vivo definition

of the functional origin of leading strand replication on the lactococcal plasmid pFX2 Mol Gen Genet 1998, 260 (1), 38-47.

[48] Zechner, E.L., Pruger, H., Grohmann, E., Espinosa, M., Hogenauer, G. Specific cleavage of chromosomal and plasmid DNA strands in gram-positive and gram-negative bacteria can be detected with nucleotide resolution Proc Natl Acad Sci U S A 1997, 94 (14), 7435-7440.

[49] Polzleitner, E., Zechner, E.L., Renner, W., Fratte, R., Jauk, B., Hogenauer, G., Koraimann, G. TraM of plasmid R1 controls transfer gene expression as an integrated control element in a complex regulatory network Mol Microbiol 1997, 25 (3), 495-507.

[50] Grohmann, E., Zechner, E.L., Espinosa, M. Determination of specific DNA strand discontinuities with nucleotide resolution in exponentionally growing bacteria harboring rolling circle-replicating plasmids FEMS Microbiol Lett 1997, 152 (2), 363-369. S0378-1097(97)00228-0 [pii]

[51] Zechner, E.L., Wu, C.A., Marians, K.J. Coordinated leading- and lagging-strand synthesis at the Escherichia coli DNA replication fork. III. A polymerase-primase interaction governs primer size J Biol Chem 1992, 267 (6), 4054-4063.

[52] Zechner, E.L., Wu, C.A., Marians, K.J. Coordinated leading- and lagging-strand synthesis at the Escherichia coli DNA replication fork. II. Frequency of primer synthesis and efficiency of primer utilization control Okazaki fragment size J Biol Chem 1992, 267 (6), 4045-4053.

[53] Wu, C.A., Zechner, E.L., Reems, J.A., McHenry, C.S., Marians, K.J. Coordinated leading- and lagging-strand synthesis at the Escherichia coli DNA replication fork. V. Primase action regulates the cycle of Okazaki fragment synthesis J Biol Chem 1992, 267 (6), 4074-4083.

[54] Wu, C.A., Zechner, E.L., Marians, K.J. Coordinated leading- and lagging-strand synthesis at the Escherichia coli DNA replication fork. I. Multiple effectors act to modulate Okazaki fragment size J Biol Chem 1992, 267 (6), 4030-4044.

[55] Wu, C.A., Zechner, E.L., Hughes, A.J., Jr., Franden, M.A., McHenry, C.S., Marians, K.J. Coordinated leading- and lagging-strand synthesis at the Escherichia coli DNA replication fork. IV. Reconstitution of an asymmetric, dimeric DNA polymerase III holoenzyme J Biol Chem 1992, 267 (6), 4064-4073.

[56] Russell, D.S., Gherzi, R., Johnson, E.L., Chou, C.K., Rosen, O.M. The protein-tyrosine kinase activity of the insulin receptor is necessary for insulin-mediated receptor down-regulation J Biol Chem 1987, 262 (24), 11833-11840.

CV – Zechner R.

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Curriculum Vitae – Univ.-Prof. Dr. Rudolf Zechner Institute of Molecular Biosciences, University of Graz, Heinrichstrasse 31, 2nd floor, 8010 Graz, Austria, Phone: +43 (0) 316 380 1900, E-Mail: [email protected] Year/ Place of Birth *1954/ Graz, Austria Nationality Austria Orcid ID 0000-0001-5483-1182 No of Publications 201 (57 first and/or corresponding) h-Index 62 (Scopus, Nov, 2016) Supervised Ph.D. Students since 2007: 6 finished, 2 ongoing Key words Biochemistry, molecular biology, cell biology of lipid and energy

metabolism


since 2016 Director BioTechMed-Graz, Austria since 2000 Professor of Biochemistry, Institute of Molecular Biosciences, University

of Graz, Austria 1998-1999 Professor of Biochemistry, Chairman of the Institute of Biochemistry,

University of Graz, Austria 1994-1998 Head of the Section of Molecular Biology, Institute of Medical

Biochemistry, University of Graz, Austria 1990-1998 Associate Professor, Institute of Medical Biochemistry, University of

Graz, Austria 1990 Habilitation in Biochemistry, Institute of Medical Biochemistry, University

of Graz, Austria 1987-1990 University Assistant, Institute of Medical Biochemistry, University of

Graz, Austria 1985-1987 Research Associate, Laboratory of Biochemical Genetics and

Metabolism, The Rockefeller University, New York, USA 1980-1984 University Assistant, Institute of Medical Biochemistry, University of

Graz, Austria Education

1980 Jul Ph.D. Graduation in Chemistry, University of Graz, Austria 1972-1980 University education in General Chemistry, University of Graz, Austria Honors ▪ Awards 2015 Louis-Jeantet Prize for Medicine 2014 Richard Havel Award Lecture at The Deuel Lipid Conference, San

Diego, CA, USA 2013 Science Award, sponsored by the Country of Styria 2013 ERC Advanced Grant by the European Research Council 2012 Kardina-Innitzer-Würdigungspreis, sponsored by the Kardinal-Innitzer

Studienfonds, Austria 2012 Award Lecture: DeWitt S. Goodman Lectureship, Columbia University,

NY, USA 2012 Award Lecture: Van Deenen Lecture, International Conference on the

Bioscience of Lipids der American Society of Biochemistry and Molecular Biology, Banff, Canada

2011 Award Lecture: The Journal of Lipid Research Lectureship Award, Kern Lipid Conference, Vail, Colorado, USA

2009 European Lipid Science Award, sponsored by the European Federartion for the Science and Technology of Lipids


CV – Zechner R.

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2007 Wittgenstein Award, sponsored by the Austrian Federal Ministry of Science and Research

1990 Science Award, sponsored by the County of Styria 1990 Kuner-Science Award, sponsored by Unilever 1986 Erwin-Schroedinger Stipendium, sponsored by the Austrian Science

Fund FWF 1985 Max-Kade Fellowship, sponsored by the Max Kade Foundation Career related activities since 2012 Member of the Editorial Board of Cell Metabolism since 2010 Executive Editor: BBA-Molecular and Cellular Biology of Lipids since 2008 Member of the Austrian Academy of Sciences since 2007 Speaker of the Research Consortium SFB LIPOTOX, Graz, Austria since 2004 Member of the Editorial Board of the Journal of Lipid Research since 2004 Corresponding member of the Austrian Academy of Sciences since 2002 Project Coordinator for the GEN-AU project “GOLD – Genomics of Lipid-

associated Disorders” 1998-2004 Chairman of the Natural Science Faculty, University of Graz, Austria since 1999 Vice President of the Austrian Atherosclerosis Society 1997-2005 Speaker of the Research Consortium SFB Biomembranes, Graz, Austria

Externally Funded National and International Projects (since 2011)

Funding Org.

Project Number


Funding Period

Austrian Science

Fund, FWF F30

SFB LIPOTOX - Lipases and the Control of Energy

Balance, Insulin Resistance, and Lipotoxicity (Coordinator & PI) - 1st, 2nd and 3rd Funding

Period

3800 (12.5 Mio for consortium)

2007-2017

Austrian Science

Fund, FWF Z136

Wittgenstein Award: Metabolic Lipase in Energy and Lipid

Metabolism 1500 2008-2014

Foundation Leducq

12 CVD 04

Transatlantic Network on Triglycerides - Triglyceride Metabolism in Obesity and

Cardiovascular Disease (Coordinator & PI)

665 (12 Mio for consortium)

2013-2017

European Research Council (ERC)

ERC-2013-ADG

340896

ERC Advanced Grant: The Role of Lipolysis in the

Pathogenesis of Cancer-associated Cachexia

2500 2014-2019

GEN-AU - Genome

Research in Austria/FFG

FFG-820979 GOLD I-III - Genomics Of Lipid-associated Disorders

(Coordinator & PI)

3000 (11 Mio for consortium)

2002-2012

Publications

[1] Grond S, Eichmann TO, Dubrac S, et al. PNPLA1 deficiency in mice and humans leads to a defect in the synthesis of ω-O-acylceramides J Invest Dermatol 2016. 10.1016/j.jid.2016.08.036

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[2] Grond S, Radner FPW, Eichmann TO, et al. Skin Barrier Development Depends on CGI-58 Protein Expression During Late-Stage Keratinocyte Differentiation J Invest Dermatol 2016. 10.1016/j.jid.2016.09.025

[3] Grumet L, Eichmann TO, Taschler U, et al. Lysosomal Acid Lipase Hydrolyzes Retinyl Ester and Affects Retinoid Turnover J Biol Chem 2016, 291(34), 17977-17987. 10.1074/jbc.M116.724054

[4] Heier C, Taschler U, Radulovic M, et al. Monoacylglycerol Lipases Act as Evolutionarily Conserved Regulators of Non-oxidative Ethanol Metabolism J Biol Chem 2016, 291(22), 11865-11875. 10.1074/jbc.M115.705541

[5] Kory N, Grond S, Kamat SS, et al. Mice Lacking Lipid Droplet-Associated Hydrolase, a Gene Linked to Human Prostate Cancer, Have Normal Cholesterol Ester Metabolism J Lipid Res November 2016jlr.M072538. 10.1194/jlr.M072538

[6] Lord CC, Ferguson D, Thomas G, et al. Regulation of Hepatic Triacylglycerol Metabolism by CGI-58 Does Not Require ATGL Co-activation Cell Rep 2016, 16(4), 939-949. 10.1016/j.celrep.2016.06.049

[7] Boeszoermenyi A, Nagy HM, Arthanari H, et al. Structure of a CGI-58 Motif Provides the Molecular Basis of Lipid Droplet Anchoring J Biol Chem 2015, 290(44), 26361-26372. 10.1074/jbc.M115.682203

[8] Das S, Stadelmeyer E, Schauer S, et al. Micro RNA-124a Regulates Lipolysis via Adipose Triglyceride Lipase and Comparative Gene Identification 58 Int J Mol Sci 2015, 16(4), 8555-8568. 10.3390/ijms16048555

[9] Eichmann TO, Grumet L, Taschler U, et al. ATGL and CGI-58 are lipid droplet proteins of the hepatic stellate cell line HSC-T6 J Lipid Res 2015, 56(10), 1972-1984. 10.1194/jlr.M062372

[10] Folick A, Oakley HD, Yu Y, et al. Lysosomal signaling molecules regulate longevity in Caenorhabditis elegans Science 2015, 347(6217), 83-86. 10.1126/science.1258857

[11] Heier C, Radner FPW, Moustafa T, et al. G0/G1 Switch Gene 2 Regulates Cardiac Lipolysis J Biol Chem 2015, 290(43), 26141-26150. 10.1074/jbc.M115.671842

[12] Hofer P, Boeszoermenyi A, Jaeger D, et al. Fatty Acid-binding Proteins Interact with Comparative Gene Identification-58 Linking Lipolysis with Lipid Ligand Shuttling J Biol Chem 2015, 290(30), 18438-18453. 10.1074/jbc.M114.628958

[13] Jaeger D, Schoiswohl G, Hofer P, et al. Fasting-induced G0/G1 switch gene 2 and FGF21 expression in the liver are under regulation of adipose tissue derived fatty acids J Hepatol 2015, 63(2), 437-445. 10.1016/j.jhep.2015.02.035

[14] Mayer N, Schweiger M, Melcher M-C, et al. Structure–activity studies in the development of a hydrazone based inhibitor of adipose-triglyceride lipase (ATGL) Bioorg Med Chem 2015, 23(12), 2904-2916. 10.1016/j.bmc.2015.02.051

[15] Pollak NM, Jaeger D, Kolleritsch S, et al. The Interplay of Protein Kinase A and Perilipin 5 Regulates Cardiac Lipolysis J Biol Chem 2015, 290(3), 1295-1306. 10.1074/jbc.M114.604744

[16] Savonen R, Hiden M, Hultin M, et al. The tissue distribution of lipoprotein lipase determines where chylomicrons bind J Lipid Res 2015, 56(3), 588-598. 10.1194/jlr.M056028

[17] Schreiber R, Hofer P, Taschler U, et al. Hypophagia and metabolic adaptations in mice with defective ATGL-mediated lipolysis cause resistance to HFD-induced obesity Proc Natl Acad Sci 2015, 112(45), 13850-13855. 10.1073/pnas.1516004112

[18] Schweiger M, Zechner R. Breaking the Barrier—Chaperone-Mediated Autophagy of Perilipins Regulates the Lipolytic Degradation of Fat Cell Metab 2015, 22(1), 60-61. 10.1016/j.cmet.2015.06.017

[19] Taschler U, Schreiber R, Chitraju C, et al. Adipose triglyceride lipase is involved in the mobilization of triglyceride and retinoid stores of hepatic stellate cells Biochim Biophys Acta - Mol Cell Biol Lipids 2015, 1851(7), 937-945. 10.1016/j.bbalip.2015.02.017

[20] Weinert BT, Iesmantavicius V, Moustafa T, et al. Acetylation dynamics and stoichiometry in Saccharomyces cerevisiae Mol Syst Biol 2015, 11(10), 833-833. 10.15252/msb.156513

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[21] Weinert BT, Moustafa T, Iesmantavicius V, Zechner R, Choudhary C. Analysis of acetylation stoichiometry suggests that SIRT3 repairs nonenzymatic acetylation lesions EMBO J 2015, 34(21), 2620-2632. 10.15252/embj.201591271

[22] Zechner R. FAT FLUX: enzymes, regulators, and pathophysiology of intracellular lipolysis EMBO Mol Med 2015, 7(4), 359-362. 10.15252/emmm.201404846

[23] Al-Zoughbi W, Pichler M, Gorkiewicz G, et al. Loss of Adipose Triglyceride Lipase is associated with human cancer and induces mouse pulmonary neoplasia Oncotarget November 2014. 10.18632/oncotarget.9418

[24] Brahma MK, Adam RC, Pollak NM, et al. Fibroblast growth factor 21 is induced upon cardiac stress and alters cardiac lipid homeostasis J Lipid Res 2014, 55(11), 2229-2241. 10.1194/jlr.M044784

[25] Camus G, Schweiger M, Herker E, et al. The Hepatitis C Virus Core Protein Inhibits Adipose Triglyceride Lipase (ATGL)-mediated Lipid Mobilization and Enhances the ATGL Interaction with Comparative Gene Identification 58 (CGI-58) and Lipid Droplets J Biol Chem 2014, 289(52), 35770-35780. 10.1074/jbc.M114.587816

[26] Cerk IK, Salzburger B, Boeszoermenyi A, et al. A Peptide Derived from G0/G1 Switch Gene 2 Acts as Noncompetitive Inhibitor of Adipose Triglyceride Lipase J Biol Chem 2014, 289(47), 32559-32570. 10.1074/jbc.M114.602599

[27] Grahn THM, Kaur R, Yin J, et al. Fat-specific Protein 27 (FSP27) Interacts with Adipose Triglyceride Lipase (ATGL) to Regulate Lipolysis and Insulin Sensitivity in Human Adipocytes J Biol Chem 2014, 289(17), 12029-12039. 10.1074/jbc.M113.539890

[28] Jha P, Claudel T, Baghdasaryan A, et al. Role of adipose triglyceride lipase (PNPLA2) in protection from hepatic inflammation in mouse models of steatohepatitis and endotoxemia Hepatology 2014, 59(3), 858-869. 10.1002/hep.26732

[29] Kotzbeck P, Zechner R. Angiopoietin-like 4: An endogenous break of intestinal lipid digestion Mol Metab 2014, 3(2), 88-89. 10.1016/j.molmet.2014.01.009

[30] Mattijssen F, Georgiadi A, Andasarie T, et al. Hypoxia-inducible Lipid Droplet-associated (HILPDA) Is a Novel Peroxisome Proliferator-activated Receptor (PPAR) Target Involved in Hepatic Triglyceride Secretion J Biol Chem 2014, 289(28), 19279-19293. 10.1074/jbc.M114.570044

[31] Mussbacher M, Stessel H, Wölkart G, et al. Role of the ubiquitin–proteasome system in cardiac dysfunction of adipose triglyceride lipase-deficient mice J Mol Cell Cardiol 2014, 7711-19. 10.1016/j.yjmcc.2014.09.028

[32] Nagy HM, Paar M, Heier C, et al. Adipose triglyceride lipase activity is inhibited by long-chain acyl-coenzyme A Biochim Biophys Acta - Mol Cell Biol Lipids 2014, 1841(4), 588-594. 10.1016/j.bbalip.2014.01.005

[33] Petruzzelli M, Schweiger M, Schreiber R, et al. A Switch from White to Brown Fat Increases Energy Expenditure in Cancer-Associated Cachexia Cell Metab 2014, 20(3), 433-447. 10.1016/j.cmet.2014.06.011

[34] Schrammel A, Mussbacher M, Wölkart G, et al. Endothelial dysfunction in adipose triglyceride lipase deficiency Biochim Biophys Acta - Mol Cell Biol Lipids 2014, 1841(6), 906-917. 10.1016/j.bbalip.2014.03.005

[35] Schreiber R, Zechner R. Lipolysis meets inflammation: arachidonic acid mobilization from fat J Lipid Res 2014, 55(12), 2447-2449. 10.1194/jlr.C055673

[36] Schweiger M, Eichmann TO, Taschler U, Zimmermann R, Zechner R, Lass A. Measurement of Lipolysis Methods Enzymol 2014:171-193. 10.1016/B978-0-12-800280-3.00010-4

[37] Tsoli M, Schweiger M, Vanniasinghe AS, et al. Depletion of White Adipose Tissue in Cancer Cachexia Syndrome Is Associated with Inflammatory Signaling and Disrupted Circadian Regulation PLoS One 2014, 9(3), e92966. 10.1371/journal.pone.0092966

[38] Zechner R, Langin D. Hormone-Sensitive Lipase Deficiency in Humans Cell Metab 2014, 20(2), 199-201. 10.1016/j.cmet.2014.07.018

[39] Zierler KA, Zechner R, Haemmerle G. Comparative gene identification-58/α/β hydrolase domain 5 Curr Opin Lipidol 2014, 25(2), 102-109. 10.1097/MOL.0000000000000058

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[40] Chitraju C, Trotzmuller M, Hartler J, et al. The impact of genetic stress by ATGL deficiency on the lipidome of lipid droplets from murine hepatocytes J Lipid Res 2013, 54(8), 2185-2194. 10.1194/jlr.M037952

[41] Currie E, Schulze A, Zechner R, Walther TC, Farese RV. Cellular Fatty Acid Metabolism and Cancer Cell Metab 2013, 18(2), 153-161. 10.1016/j.cmet.2013.05.017

[42] Kienesberger PC, Pulinilkunnil T, Nagendran J, et al. Early structural and metabolic cardiac remodelling in response to inducible adipose triglyceride lipase ablation Cardiovasc Res 2013, 99(3), 442-451. 10.1093/cvr/cvt124

[43] Mayer N, Schweiger M, Romauch M, et al. Development of small-molecule inhibitors targeting adipose triglyceride lipase Nat Chem Biol 2013, 9(12), 785-787. 10.1038/nchembio.1359

[44] Pollak NM, Schweiger M, Jaeger D, et al. Cardiac-specific overexpression of perilipin 5 provokes severe cardiac steatosis via the formation of a lipolytic barrier J Lipid Res 2013, 54(4), 1092-1102. 10.1194/jlr.M034710

[45] Pulinilkunnil T, Kienesberger PC, Nagendran J, et al. Myocardial Adipose Triglyceride Lipase Overexpression Protects Diabetic Mice From the Development of Lipotoxic Cardiomyopathy Diabetes 2013, 62(5), 1464-1477. 10.2337/db12-0927

[46] Schrammel A, Mussbacher M, Winkler S, et al. Cardiac oxidative stress in a mouse model of neutral lipid storage disease Biochim Biophys Acta - Mol Cell Biol Lipids 2013, 1831(11), 1600-1608. 10.1016/j.bbalip.2013.07.004

[47] Sitnick MT, Basantani MK, Cai L, et al. Skeletal Muscle Triacylglycerol Hydrolysis Does Not Influence Metabolic Complications of Obesity Diabetes 2013, 62(10), 3350-3361. 10.2337/db13-0500

[48] van de Weijer T, Havekes B, Bilet L, et al. Effects of Bezafibrate Treatment in a Patient and a Carrier With Mutations in the PNPLA2 Gene, Causing Neutral Lipid Storage Disease With Myopathy Circ Res 2013, 112(5), e51-e54. 10.1161/CIRCRESAHA.113.300944

[49] Yasmeen R, Reichert B, Deiuliis J, et al. Autocrine Function of Aldehyde Dehydrogenase 1 as a Determinant of Diet- and Sex-Specific Differences in Visceral Adiposity Diabetes 2013, 62(1), 124-136. 10.2337/db11-1779

[50] Young SG, Zechner R. Biochemistry and pathophysiology of intravascular and intracellular lipolysis Genes Dev 2013, 27(5), 459-484. 10.1101/gad.209296.112

[51] Zierler KA, Jaeger D, Pollak NM, et al. Functional Cardiac Lipolysis in Mice Critically Depends on Comparative Gene Identification-58 J Biol Chem 2013, 288(14), 9892-9904. 10.1074/jbc.M112.420620

[52] Chitraju C, Trotzmuller M, Hartler J, et al. Lipidomic analysis of lipid droplets from murine hepatocytes reveals distinct signatures for nutritional stress J Lipid Res 2012, 53(10), 2141-2152. 10.1194/jlr.M028902

[53] Eichmann TO, Kumari M, Haas JT, et al. Studies on the Substrate and Stereo/Regioselectivity of Adipose Triglyceride Lipase, Hormone-sensitive Lipase, and Diacylglycerol-O-acyltransferases J Biol Chem 2012, 287(49), 41446-41457. 10.1074/jbc.M112.400416

[54] Farese RV, Zechner R, Newgard CB, Walther TC. The Problem of Establishing Relationships between Hepatic Steatosis and Hepatic Insulin Resistance Cell Metab 2012, 15(5), 570-573. 10.1016/j.cmet.2012.03.004

[55] Grall A, Guaguère E, Planchais S, et al. PNPLA1 mutations cause autosomal recessive congenital ichthyosis in golden retriever dogs and humans Nat Genet 2012, 44(2), 140-147. 10.1038/ng.1056

[56] Kienesberger PC, Pulinilkunnil T, Sung MMY, et al. Myocardial ATGL Overexpression Decreases the Reliance on Fatty Acid Oxidation and Protects against Pressure Overload-Induced Cardiac Dysfunction Mol Cell Biol 2012, 32(4), 740-750. 10.1128/MCB.06470-11

[57] Kumari M, Schoiswohl G, Chitraju C, et al. Adiponutrin Functions as a Nutritionally Regulated Lysophosphatidic Acid Acyltransferase Cell Metab 2012, 15(5), 691-702. 10.1016/j.cmet.2012.04.008

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[58] Lasa A, Schweiger M, Kotzbeck P, et al. Resveratrol regulates lipolysis via adipose triglyceride lipase J Nutr Biochem 2012, 23(4), 379-384. 10.1016/j.jnutbio.2010.12.014

[59] Morak M, Schmidinger H, Riesenhuber G, et al. Adipose Triglyceride Lipase (ATGL) and Hormone-Sensitive Lipase (HSL) Deficiencies Affect Expression of Lipolytic Activities in Mouse Adipose Tissues Mol Cell Proteomics 2012, 11(12), 1777-1789. 10.1074/mcp.M111.015743

[60] Moustafa T, Fickert P, Magnes C, et al. Alterations in Lipid Metabolism Mediate Inflammation, Fibrosis, and Proliferation in a Mouse Model of Chronic Cholestatic Liver Injury Gastroenterology 2012, 142(1), 140-151.e12. 10.1053/j.gastro.2011.09.051

[61] Schweiger M, Paar M, Eder C, et al. G0/G1 switch gene-2 regulates human adipocyte lipolysis by affecting activity and localization of adipose triglyceride lipase J Lipid Res 2012, 53(11), 2307-2317. 10.1194/jlr.M027409

[62] Shimizu I, Yoshida Y, Katsuno T, et al. p53-Induced Adipose Tissue Inflammation Is Critically Involved in the Development of Insulin Resistance in Heart Failure Cell Metab 2012, 15(1), 51-64. 10.1016/j.cmet.2011.12.006

[63] Wölkart G, Schrammel A, Dörffel K, Haemmerle G, Zechner R, Mayer B. Cardiac dysfunction in adipose triglyceride lipase deficiency: treatment with a PPARα agonist Br J Pharmacol 2012, 165(2), 380-389. 10.1111/j.1476-5381.2011.01490.x

[64] Zechner R, Zimmermann R, Eichmann TO, et al. FAT SIGNALS - Lipases and Lipolysis in Lipid Metabolism and Signaling Cell Metab 2012, 15(3), 279-291. 10.1016/j.cmet.2011.12.018

[65] Aflaki E, Radovic B, Chandak PG, et al. Triacylglycerol Accumulation Activates the Mitochondrial Apoptosis Pathway in Macrophages J Biol Chem 2011, 286(9), 7418-7428. 10.1074/jbc.M110.175703

[66] Basantani MK, Sitnick MT, Cai L, et al. Pnpla3/Adiponutrin deficiency in mice does not contribute to fatty liver disease or metabolic syndrome J Lipid Res 2011, 52(2), 318-329. 10.1194/jlr.M011205

[67] Borg ML, Andrews ZB, Duh EJ, Zechner R, Meikle PJ, Watt MJ. Pigment Epithelium-Derived Factor Regulates Lipid Metabolism via Adipose Triglyceride Lipase Diabetes 2011, 60(5), 1458-1466. 10.2337/db10-0845

[68] Cornaciu I, Boeszoermenyi A, Lindermuth H, et al. The Minimal Domain of Adipose Triglyceride Lipase (ATGL) Ranges until Leucine 254 and Can Be Activated and Inhibited by CGI-58 and G0S2, Respectively PLoS One 2011, 6(10), e26349. 10.1371/journal.pone.0026349

[69] Das SK, Eder S, Schauer S, et al. Adipose Triglyceride Lipase Contributes to Cancer-Associated Cachexia Science 2011, 333(6039), 233-238. 10.1126/science.1198973

[70] Etschmaier K, Becker T, Eichmann TO, et al. Adipose triglyceride lipase affects triacylglycerol metabolism at brain barriers J Neurochem 2011, 119(5), 1016-1028. 10.1111/j.1471-4159.2011.07498.x

[71] Haemmerle G, Moustafa T, Woelkart G, et al. ATGL-mediated fat catabolism regulates cardiac mitochondrial function via PPAR-α and PGC-1 Nat Med 2011, 17(9), 1076-1085. 10.1038/nm.2439

[72] Harris CA, Haas JT, Streeper RS, et al. DGAT enzymes are required for triacylglycerol synthesis and lipid droplets in adipocytes J Lipid Res 2011, 52(4), 657-667. 10.1194/jlr.M013003

[73] Lammers B, Chandak PG, Aflaki E, et al. Macrophage Adipose Triglyceride Lipase Deficiency Attenuates Atherosclerotic Lesion Development in Low-Density Lipoprotein Receptor Knockout Mice Arterioscler Thromb Vasc Biol 2011, 31(1), 67-73. 10.1161/ATVBAHA.110.215814

[74] Lass A, Zimmermann R, Oberer M, Zechner R. Lipolysis – A highly regulated multi-enzyme complex mediates the catabolism of cellular fat stores Prog Lipid Res 2011, 50(1), 14-27. 10.1016/j.plipres.2010.10.004

[75] Mueller KM, Kornfeld J-W, Friedbichler K, et al. Impairment of hepatic growth hormone and glucocorticoid receptor signaling causes steatosis and hepatocellular carcinoma in mice Hepatology 2011, 54(4), 1398-1409. 10.1002/hep.24509

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[76] Oberer M, Boeszoermenyi A, Nagy HM, Zechner R. Recent insights into the structure and function of comparative gene identification-58 Curr Opin Lipidol 2011, 22(3), 149-158. 10.1097/MOL.0b013e328346230e

[77] Radner FPW, Grond S, Lass A, Zechner R. Fat in the skin Dermatoendocrinol 2011, 3(2), 77-83. 10.4161/derm.3.2.15472

[78] Scherer T, O’Hare J, Diggs-Andrews K, et al. Brain Insulin Controls Adipose Tissue Lipolysis and Lipogenesis Cell Metab 2011, 13(2), 183-194. 10.1016/j.cmet.2011.01.008

[79] Taschler U, Radner FPW, Heier C, et al. Monoglyceride Lipase Deficiency in Mice Impairs Lipolysis and Attenuates Diet-induced Insulin Resistance J Biol Chem 2011, 286(20), 17467-17477. 10.1074/jbc.M110.215434

[80] Buchebner M, Pfeifer T, Rathke N, et al. Cholesteryl ester hydrolase activity is abolished in HSL macrophages but unchanged in macrophages lacking KIAA1363 J Lipid Res 2010, 51(10), 2896-2908. 10.1194/jlr.M004259

[81] Chandak PG, Radovic B, Aflaki E, et al. Efficient Phagocytosis Requires Triacylglycerol Hydrolysis by Adipose Triglyceride Lipase J Biol Chem 2010, 285(26), 20192-20201. 10.1074/jbc.M110.107854

[82] Coassin S, Schweiger M, Kloss-Brandstätter A, et al. Investigation and Functional Characterization of Rare Genetic Variants in the Adipose Triglyceride Lipase in a Large Healthy Working Population PLoS Genet 2010, 6(12), e1001239. 10.1371/journal.pgen.1001239

[83] Gorkiewicz G, Kienesberger S, Schober C, et al. A Genomic Island Defines Subspecies-Specific Virulence Features of the Host-Adapted Pathogen Campylobacter fetus subsp. venerealis J Bacteriol 2010, 192(2), 502-517. 10.1128/JB.00803-09

[84] Gruber A, Cornaciu I, Lass A, et al. The N-terminal Region of Comparative Gene Identification-58 (CGI-58) Is Important for Lipid Droplet Binding and Activation of Adipose Triglyceride Lipase J Biol Chem 2010, 285(16), 12289-12298. 10.1074/jbc.M109.064469

[85] Holmes RS, Wright MW, Laulederkind SJF, et al. Recommended nomenclature for five mammalian carboxylesterase gene families: human, mouse, and rat genes and proteins Mamm Genome 2010, 21(9-10), 427-441. 10.1007/s00335-010-9284-4

[86] Kosteli A, Sugaru E, Haemmerle G, et al. Weight loss and lipolysis promote a dynamic immune response in murine adipose tissue J Clin Invest 2010, 120(10), 3466-3479. 10.1172/JCI42845

[87] Radner FPW, Streith IE, Schoiswohl G, et al. Growth Retardation, Impaired Triacylglycerol Catabolism, Hepatic Steatosis, and Lethal Skin Barrier Defect in Mice Lacking Comparative Gene Identification-58 (CGI-58) J Biol Chem 2010, 285(10), 7300-7311. 10.1074/jbc.M109.081877

[88] Schoiswohl G, Schweiger M, Schreiber R, et al. Adipose triglyceride lipase plays a key role in the supply of the working muscle with fatty acids J Lipid Res 2010, 51(3), 490-499. 10.1194/jlr.M001073

[89] Fex M, Haemmerle G, Wierup N, et al. A beta cell-specific knockout of hormone-sensitive lipase in mice results in hyperglycaemia and disruption of exocytosis Diabetologia 2009, 52(2), 271-280. 10.1007/s00125-008-1191-9

[90] Huijsman E, van de Par C, Economou C, et al. Adipose triacylglycerol lipase deletion alters whole body energy metabolism and impairs exercise performance in mice AJP Endocrinol Metab 2009, 297(2), E505-E513. 10.1152/ajpendo.00190.2009

[91] Kienesberger PC, Lee D, Pulinilkunnil T, et al. Adipose Triglyceride Lipase Deficiency Causes Tissue-specific Changes in Insulin Signaling J Biol Chem 2009, 284(44), 30218-30229. 10.1074/jbc.M109.047787

[92] Klar J, Schweiger M, Zimmerman R, et al. Mutations in the Fatty Acid Transport Protein 4 Gene Cause the Ichthyosis Prematurity Syndrome Am J Hum Genet 2009, 85(2), 248-253. 10.1016/j.ajhg.2009.06.021

[93] Peyot M-L, Guay C, Latour MG, et al. Adipose Triglyceride Lipase Is Implicated in Fuel- and Non-fuel-stimulated Insulin Secretion J Biol Chem 2009, 284(25), 16848-16859. 10.1074/jbc.M109.006650

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[94] Riederer M, Erwa W, Zimmermann R, Frank S, Zechner R. Adipose tissue as a source of nicotinamide N-methyltransferase and homocysteine Atherosclerosis 2009, 204(2), 412-417. 10.1016/j.atherosclerosis.2008.09.015

[95] Schreiber R, Taschler U, Wolinski H, et al. Esterase 22 and beta-glucuronidase hydrolyze retinoids in mouse liver J Lipid Res 2009, 50(12), 2514-2523. 10.1194/jlr.M000950

[96] Schweiger M, Lass A, Zimmermann R, Eichmann TO, Zechner R. Neutral lipid storage disease: genetic disorders caused by mutations in adipose triglyceride lipase/PNPLA2 or CGI-58/ABHD5 AJP Endocrinol Metab 2009, 297(2), E289-E296. 10.1152/ajpendo.00099.2009

[97] Zechner R, Kienesberger PC, Haemmerle G, Zimmermann R, Lass A. Adipose triglyceride lipase and the lipolytic catabolism of cellular fat stores J Lipid Res 2009, 50(1), 3-21. 10.1194/jlr.R800031-JLR200

[98] Zechner R, Madeo F. Cell biology: Another way to get rid of fat Nature 2009, 458(7242), 1118-1119. 10.1038/4581118a

[99] Zimmermann R, Lass A, Haemmerle G, Zechner R. Fate of fat: The role of adipose triglyceride lipase in lipolysis Biochim Biophys Acta - Mol Cell Biol Lipids 2009, 1791(6), 494-500. 10.1016/j.bbalip.2008.10.005

[100] Alsted TJ, Nybo L, Schweiger M, et al. Adipose triglyceride lipase in human skeletal muscle is upregulated by exercise training AJP Endocrinol Metab 2008, 296(3), E445-E453. 10.1152/ajpendo.90912.2008

[101] Kienesberger PC, Oberer M, Lass A, Zechner R. Mammalian patatin domain containing proteins: a family with diverse lipolytic activities involved in multiple biological functions J Lipid Res 2008, 50(Supplement), S63-S68. 10.1194/jlr.R800082-JLR200

[102] Kienesberger PC, Lass A, Preiss-Landl K, et al. Identification of an insulin-regulated lysophospholipase with homology to neuropathy target esterase J Biol Chem 2008, 283(9), 5908-5917. 10.1074/jbc.M709598200

[103] Pinent M, Hackl H, Burkard TR, et al. Differential transcriptional modulation of biological processes in adipocyte triglyceride lipase and hormone-sensitive lipase-deficient mice. Genomics 2008, 92(1), 26-32. 10.1016/j.ygeno.2008.03.010

[104] Reid BN, Ables GP, Otlivanchik OA, et al. Hepatic Overexpression of Hormone-sensitive Lipase and Adipose Triglyceride Lipase Promotes Fatty Acid Oxidation, Stimulates Direct Release of Free Fatty Acids, and Ameliorates Steatosis J Biol Chem 2008, 283(19), 13087-13099. 10.1074/jbc.M800533200

[105] Schweiger M, Schoiswohl G, Lass A, et al. The C-terminal Region of Human Adipose Triglyceride Lipase Affects Enzyme Activity and Lipid Droplet Binding J Biol Chem 2008, 283(25), 17211-17220. 10.1074/jbc.M710566200

[106] Haemmerle G, Lass A, Zimmermann R, et al. Defective lipolysis and altered energy metabolism in mice lacking adipose triglyceride lipase Science 2006, 312(5774), 734-737. 10.1126/science.1123965

[107] Kurat CF, Natter K, Petschnigg J, et al. Obese yeast: triglyceride lipolysis is functionally conserved from mammals to yeast J Biol Chem 2006, 281(1), 491-500. 10.1074/jbc.M508414200

[108] Lass A, Zimmermann R, Haemmerle G, et al. Adipose triglyceride lipase-mediated lipolysis of cellular fat stores is activated by CGI-58 and defective in Chanarin-Dorfman Syndrome Cell Metab 2006, 3(5), 309-319. 10.1016/j.cmet.2006.03.005

[109] Osibow K, Frank S, Malli R, Zechner R, Graier WF. Mitochondria maintain maturation and secretion of lipoprotein lipase in the endoplasmic reticulum Biochem J 2006, 396(1), 173-182. 10.1042/BJ20060099

[110] Schoenborn V, Heid IM, Vollmert C, et al. The ATGL gene is associated with free fatty acids, triglycerides, and type 2 diabetes Diabetes 2006, 55(5), 1270-1275

[111] Schweiger M, Schreiber R, Haemmerle G, et al. Adipose triglyceride lipase and hormone-sensitive lipase are the major enzymes in adipose tissue triacylglycerol catabolism J Biol Chem 2006, 281(52), 40236-40241. 10.1074/jbc.M608048200

[112] Tschernatsch MMO, Mlecnik B, Trajanoski Z, Zechner R, Zimmermann R. LPL-mediated lipolysis of VLDL induces an upregulation of AU-rich mRNAs and an activation

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of HuR in endothelial cells Atherosclerosis 2006, 189(2), 310-317. 10.1016/j.atherosclerosis.2006.01.007

[113] Birner-Gruenberger R, Susani-Etzerodt H, Waldhuber M, et al. The lipolytic proteome of mouse adipose tissue Mol Cell Proteomics 2005, 4(11), 1710-1717. 10.1074/mcp.M500062-MCP200

[114] Kratky D, Zimmermann R, Wagner EM, et al. Endothelial lipase provides an alternative pathway for FFA uptake in lipoprotein lipase-deficient mouse adipose tissue J Clin Invest 2005, 115(1), 161-167. 10.1172/JCI15972

[115] Lindegaard MLS, Olivecrona G, Christoffersen C, et al. Endothelial and lipoprotein lipases in human and mouse placenta J Lipid Res 2005, 46(11), 2339-2346. 10.1194/jlr.M500277-JLR200

[116] Zechner R, Strauss JG, Haemmerle G, Lass A, Zimmermann R. Lipolysis: pathway under construction Curr Opin Lipidol 2005, 16(3), 333-340

[117] Augustus A, Yagyu H, Haemmerle G, et al. Cardiac-specific knock-out of lipoprotein lipase alters plasma lipoprotein triglyceride metabolism and cardiac gene expression J Biol Chem 2004, 279(24), 25050-25057. 10.1074/jbc.M401028200

[118] Fritz G, Wagner EM, Lindner H, Hofmann W, Zechner R, Glatter O. Dependence of lipoprotein-lipase-catalyzed triacylglycerol hydrolysis on droplet size of synthetic monodisperse emulsions measured with static light scattering J Colloid Interface Sci 2004, 275(2), 642-648. 10.1016/j.jcis.2004.02.085

[119] Wagner EM, Kratky D, Haemmerle G, et al. Defective uptake of triglyceride-associated fatty acids in adipose tissue causes the SREBP-1c-mediated induction of lipogenesis J Lipid Res 2004, 45(2), 356-365. 10.1194/jlr.M300293-JLR200

[120] Zimmermann R, Strauss JG, Haemmerle G, et al. Fat mobilization in adipose tissue is promoted by adipose triglyceride lipase Science 2004, 306(5700), 1383-1386. 10.1126/science.1100747

[121] Brunner F, Maier R, Andrew P, Wölkart G, Zechner R, Mayer B. Attenuation of myocardial ischemia/reperfusion injury in mice with myocyte-specific overexpression of endothelial nitric oxide synthase Cardiovasc Res 2003, 57(1), 55-62

[122] Gorkiewicz G, Feierl G, Schober C, et al. Species-specific identification of campylobacters by partial 16S rRNA gene sequencing J Clin Microbiol 2003, 41(6), 2537-2546

[123] Haemmerle G, Zimmermann R, Zechner R. Letting lipids go: hormone-sensitive lipase Curr Opin Lipidol 2003, 14(3), 289-297. 10.1097/01.mol.0000073509.41685.36

[124] Hensley LL, Ranganathan G, Wagner EM, et al. Transgenic mice expressing lipoprotein lipase in adipose tissue. Absence of the proximal 3’-untranslated region causes translational upregulation J Biol Chem 2003, 278(35), 32702-32709. 10.1074/jbc.M304200200

[125] Nöhammer C, Brunner F, Wölkart G, et al. Myocardial dysfunction and male mortality in peroxisome proliferator-activated receptor alpha knockout mice overexpressing lipoprotein lipase in muscle Lab Invest 2003, 83(2), 259-269

[126] Seltenhammer MH, Simhofer H, Scherzer S, et al. Equine melanoma in a population of 296 grey Lipizzaner horses Equine Vet J 2003, 35(2), 153-157

[127] Strauss JG, Hayn M, Zechner R, Levak-Frank S, Frank S. Fatty acids liberated from high-density lipoprotein phospholipids by endothelial-derived lipase are incorporated into lipids in HepG2 cells Biochem J 2003, 371(Pt 3), 981-988. 10.1042/BJ20021437

[128] Voshol PJ, Haemmerle G, Ouwens DM, et al. Increased hepatic insulin sensitivity together with decreased hepatic triglyceride stores in hormone-sensitive lipase-deficient mice. Endocrinology 2003, 144(8), 3456-3462. 10.1210/en.2002-0036

[129] Zimmermann R, Haemmerle G, Wagner EM, Strauss JG, Kratky D, Zechner R. Decreased fatty acid esterification compensates for the reduced lipolytic activity in hormone-sensitive lipase-deficient white adipose tissue J Lipid Res 2003, 44(11), 2089-2099. 10.1194/jlr.M300190-JLR200

[130] Ziouzenkova O, Perrey S, Asatryan L, et al. Lipolysis of triglyceride-rich lipoproteins generates PPAR ligands: evidence for an antiinflammatory role for lipoprotein lipase Proc Natl Acad Sci USA 2003, 100(5), 2730-2735. 10.1073/pnas.0538015100

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[131] Esenabhalu VE, Cerimagic M, Malli R, et al. Tissue-specific expression of human lipoprotein lipase in the vascular system affects vascular reactivity in transgenic mice. Br J Pharmacol 2002, 135(1), 143-154. 10.1038/sj.bjp.0704440

[132] Gorkiewicz G, Feierl G, Zechner R, Zechner EL. Transmission of Campylobacter hyointestinalis from a pig to a human J Clin Microbiol 2002, 40(7), 2601-2605

[133] Goti D, Balazs Z, Panzenboeck U, et al. Effects of lipoprotein lipase on uptake and transcytosis of low density lipoprotein (LDL) and LDL-associated alpha-tocopherol in a porcine in vitro blood-brain barrier model J Biol Chem 2002, 277(32), 28537-28544. 10.1074/jbc.M203989200

[134] Haemmerle G, Zimmermann R, Hayn M, et al. Hormone-sensitive lipase deficiency in mice causes diglyceride accumulation in adipose tissue, muscle, and testis J Biol Chem 2002, 277(7), 4806-4815. 10.1074/jbc.M110355200

[135] Haemmerle G, Zimmermann R, Strauss JG, et al. Hormone-sensitive lipase deficiency in mice changes the plasma lipid profile by affecting the tissue-specific expression pattern of lipoprotein lipase in adipose tissue and muscle J Biol Chem 2002, 277(15), 12946-12952. 10.1074/jbc.M108640200

[136] Merkel M, Heeren J, Dudeck W, et al. Inactive lipoprotein lipase (LPL) alone increases selective cholesterol ester uptake in vivo, whereas in the presence of active LPL it also increases triglyceride hydrolysis and whole particle lipoprotein uptake J Biol Chem 2002, 277(9), 7405-7411. 10.1074/jbc.M107914200

[137] Preiss-Landl K, Zimmermann R, Hämmerle G, Zechner R. Lipoprotein lipase: the regulation of tissue specific expression and its role in lipid and energy metabolism Curr Opin Lipidol 2002, 13(5), 471-481

[138] Strauss JG, Zimmermann R, Hrzenjak A, et al. Endothelial cell-derived lipase mediates uptake and binding of high-density lipoprotein (HDL) particles and the selective uptake of HDL-associated cholesterol esters independent of its enzymic activity Biochem J 2002, 368(Pt 1), 69-79. 10.1042/BJ20020306

[139] Brunner F, Andrew P, Wölkart G, Zechner R, Mayer B. Myocardial contractile function and heart rate in mice with myocyte-specific overexpression of endothelial nitric oxide synthase Circulation 2001, 104(25), 3097-3102

[140] Kratky D, Strauss JG, Zechner R. Tissue-specific activity of lipoprotein lipase in skeletal muscle regulates the expression of uncoupling protein 3 in transgenic mouse models Biochem J 2001, 355(Pt 3), 647-652

[141] Strauss JG, Frank S, Kratky D, et al. Adenovirus-mediated rescue of lipoprotein lipase-deficient mice. Lipolysis of triglyceride-rich lipoproteins is essential for high density lipoprotein maturation in mice J Biol Chem 2001, 276(39), 36083-36090. 10.1074/jbc.M104430200

[142] Voshol PJ, Jong MC, Dahlmans VE, et al. In muscle-specific lipoprotein lipase-overexpressing mice, muscle triglyceride content is increased without inhibition of insulin-stimulated whole-body and muscle-specific glucose uptake Diabetes 2001, 50(11), 2585-2590

[143] Zimmermann R, Panzenböck U, Wintersperger A, et al. Lipoprotein lipase mediates the uptake of glycated LDL in fibroblasts, endothelial cells, and macrophages Diabetes 2001, 50(7), 1643-1653

[144] Guertl B, Beham A, Zechner R, Stammberger H, Hoefler G. Nasopharyngeal angiofibroma: an APC-gene-associated tumor? Hum Pathol 2000, 31(11), 1411-1413

[145] Meraji S, Abuja PM, Hayn M, et al. Relationship between classic risk factors, plasma antioxidants and indicators of oxidant stress in angina pectoris (AP) in Tehran Atherosclerosis 2000, 150(2), 403-412

[146] Nöhammer C, El-Shabrawi Y, Schauer S, et al. cDNA cloning and analysis of tissue-specific expression of mouse peroxisomal straight-chain acyl-CoA oxidase. Eur J Biochem 2000, 267(4), 1254-1260

[147] Prattes S, Hörl G, Hammer A, et al. Intracellular distribution and mobilization of unesterified cholesterol in adipocytes: triglyceride droplets are surrounded by cholesterol-rich ER-like surface layer structures J Cell Sci September 20002977-2989

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[148] Van Eck M, Zimmermann R, Groot PH, Zechner R, Van Berkel TJ. Role of macrophage-derived lipoprotein lipase in lipoprotein metabolism and atherosclerosis Arterioscler Thromb Vasc Biol 2000, 20(9), E53-62

[149] Zechner R, Strauss J, Frank S, et al. The role of lipoprotein lipase in adipose tissue development and metabolism Int J Obes Relat Metab Disord 2000, 24 Suppl 4S53-6

[150] Zimmermann R, Sartipy P, Winkler R, Zechner R, Hurt-Camejo E, Kostner GM. Endogenously produced glycosaminoglycans affecting the release of lipoprotein lipase from macrophages and the interaction with lipoproteins Biochim Biophys Acta 2000, 1484(2-3), 316-324

[151] Levak-Frank S, Hofmann W, Weinstock PH, et al. Induced mutant mouse lines that express lipoprotein lipase in cardiac muscle, but not in skeletal muscle and adipose tissue, have normal plasma triglyceride and high-density lipoprotein-cholesterol levels Proc Natl Acad Sci USA 1999, 96(6), 3165-3170

[152] Wang X, Greilberger J, Levak-Frank S, Zimmermann R, Zechner R, Jürgens G. Endogenously produced lipoprotein lipase enhances the binding and cell association of native, mildly oxidized and moderately oxidized low-density lipoprotein in mouse peritoneal macrophages Biochem J 1999, 343 Pt 2347-353

[153] Hoefler G, Noehammer C, Levak-Frank S, et al. Muscle-specific overexpression of human lipoprotein lipase in mice causes increased intracellular free fatty acids and induction of peroxisomal enzymes Biochimie 1997, 79(2-3), 163-168. 10.1016/S0300-9084(97)81509-X

[154] Levak-Frank S, Weinstock PH, Hayek T, et al. Induced mutant mice expressing lipoprotein lipase exclusively in muscle have subnormal triglycerides yet reduced high density lipoprotein cholesterol levels in plasma J Biol Chem 1997, 272(27), 17182-17190

[155] Panzenboeck U, Wintersberger A, Levak-Frank S, et al. Implications of endogenous and exogenous lipoprotein lipase for the selective uptake of HDL3-associated cholesteryl esters by mouse peritoneal macrophages J Lipid Res 1997, 38(2), 239-253

[156] Weinstock PH, Levak-Frank S, Hudgins LC, et al. Lipoprotein lipase controls fatty acid entry into adipose tissue, but fat mass is preserved by endogenous synthesis in mice deficient in adipose tissue lipoprotein lipase Proc Natl Acad Sci USA 1997, 94(19), 10261-10266

[157] Zechner R. The tissue-specific expression of lipoprotein lipase: implications for energy and lipoprotein metabolism Curr Opin Lipidol 1997, 8(2), 77-88

[158] Duque M, Graupner M, Stütz H, et al. New fluorogenic triacylglycerol analogs as substrates for the determination and chiral discrimination of lipase activities J Lipid Res 1996, 37(4), 868-876

[159] Frank S, Durovic S, Zechner R, Kostner GM. Biosynthesis of lipoprotein-a from transfected cells Z Gastroenterol 1996, 34 Suppl 333-35

[160] Sattler W, Levak-Frank S, Radner H, Kostner GM, Zechner R. Muscle-specific overexpression of lipoprotein lipase in transgenic mice results in increased alpha-tocopherol levels in skeletal muscle Biochem J 1996; 15;318 ( Pt 1):15-9

[161] Steyrer E, Frank S, Durovic S, Zechner R, Kostner GM. LDL structure and Lp(a) formation: the role of lecithin:cholesterol acyltransferase Z Gastroenterol 1996, 34 Suppl 339-40

[162] Wicher I, Sattler W, Ibovnik A, Kostner GM, Zechner R, Malle E. Quantification of lipoprotein lipase (LPL) by dissociation-enhanced lanthanide fluorescence immunoassay. Comparison of immunoreactivity of LPL mass and enzyme activity of LPL J Immunol Methods 1996, 192(1-2), 1-11

[163] Levak-Frank S, Radner H, Walsh A, et al. Muscle-specific overexpression of lipoprotein lipase causes a severe myopathy characterized by proliferation of mitochondria and peroxisomes in transgenic mice J Clin Invest 1995, 96(2), 976-986. 10.1172/JCI118145

[164] Stalenhoef AF, Armstrong VW, Steinmetz A, Taskinen MR, Zechner R. The 17th annual meeting of the European Lipoprotein Club Arterioscler Thromb Vasc Biol 1995, 15(4), 543-549

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[165] Steyrer E, Haubenwallner S, Hörl G, Giessauf W, Kostner GM, Zechner R. A single G to A nucleotide transition in exon IV of the lecithin: cholesterol acyltransferase (LCAT) gene results in an Arg140 to His substitution and causes LCAT-deficiency Hum Genet 1995, 96(1), 105-109

[166] Weinstock PH, Bisgaier CL, Aalto-Setälä K, et al. Severe hypertriglyceridemia, reduced high density lipoprotein, and neonatal death in lipoprotein lipase knockout mice. Mild hypertriglyceridemia with impaired very low density lipoprotein clearance in heterozygotes J Clin Invest 1995, 96(6), 2555-2568. 10.1172/JCI118319

[167] A.F.H. S, V.W. A, S. H, G. S, A.K. S. The 16th annual meeting of the European Lipoprotein Club Arterioscler Thromb 1994, 14(5), 831-837

[168] Durovic S, März W, Frank S, et al. Decreased binding of apolipoprotein (a) to familial defective apolipoprotein B-100 (Arg3500-->Gln). A study of the assembly of recombinant apolipoprotein (a) with mutant low density lipoproteins J Biol Chem 1994, 269(48), 30320-30325

[169] Hoefler G, Forstner M, McGuinness MC, et al. cDNA cloning of the human peroxisomal enoyl-CoA hydratase: 3-hydroxyacyl-CoA dehydrogenase bifunctional enzyme and localization to chromosome 3q26.3-3q28: a free left Alu Arm is inserted in the 3’ noncoding region Genomics 1994, 19(1), 60-67

[170] Schreibmayer W, Frohnwieser B, Dascal N, et al. Beta-adrenergic modulation of currents produced by rat cardiac Na+ channels expressed in Xenopus laevis oocytes Receptors Channels 1994, 2(4), 339-350

[171] Steyrer E, Durovic S, Frank S, et al. The role of lecithin: cholesterol acyltransferase for lipoprotein (a) assembly. Structural integrity of low density lipoproteins is a prerequisite for Lp(a) formation in human plasma J Clin Invest 1994, 94(6), 2330-2340. 10.1172/JCI117598

[172] Aggerbeck LP, Angelin B, Armstrong V, et al. The 15th annual meeting of the European Lipoprotein Club Arterioscler Thromb a J Vasc Biol 1993, 13(4), 618-627

[173] Birchbauer A, Knipping G, Juritsch B, Aschauer H, Zechner R. Characterization of the apolipoprotein AI and CIII genes in the domestic pig Genomics 1993, 15(3), 643-652. 10.1006/geno.1993.1119

[174] Fried SK, Appel B, Zechner R. Interleukin 1 alpha decreases the synthesis and activity of lipoprotein lipase in human adipose tissue Horm Metab Res 1993, 25(2), 129-130. 10.1055/s-2007-1002060

[175] Haubenwallner S, Hörl G, Shachter NS, et al. A novel missense mutation in the gene for lipoprotein lipase resulting in a highly conservative amino acid substitution (Asp180-->Glu) causes familial chylomicronemia (type I hyperlipoproteinemia) Genomics 1993, 18(2), 392-396. 10.1006/geno.1993.1481

[176] Aalto-Setälä K, Fisher EA, Chen X, et al. Mechanism of hypertriglyceridemia in human apolipoprotein (apo) CIII transgenic mice. Diminished very low density lipoprotein fractional catabolic rate associated with increased apo CIII and reduced apo E on the particles J Clin Invest 1992, 90(5), 1889-1900. 10.1172/JCI116066

[177] Zechner R, Moser R, Newman TC, Fried SK, Breslow JL. Apolipoprotein E gene expression in mouse 3T3-L1 adipocytes and human adipose tissue and its regulation by differentiation and lipid content J Biol Chem 1991, 266(16), 10583-10588

[178] Zechner R, Newman TC, Steiner E, Breslow JL. The structure of the mouse lipoprotein lipase gene: a B1 repetitive element is inserted into the 3’ untranslated region of the mRNA Genomics 1991, 11(1), 62-76

[179] Zechner R. Rapid and simple isolation procedure for lipoprotein lipase from human milk Biochim Biophys Acta 1990, 1044(1), 20-25

[180] Fried SK, Zechner R. Cachectin/tumor necrosis factor decreases human adipose tissue lipoprotein lipase mRNA levels, synthesis, and activity J Lipid Res 1989, 30(12), 1917-1923.

[181] Bihari-Varga M, Gruber E, Rotheneder M, Zechner R, Kostner GM. Interaction of lipoprotein Lp(a) and low density lipoprotein with glycosaminoglycans from human aorta Arteriosclerosis 1988, 8(6), 851-857

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[182] Weintraub MS, Zechner R, Brown A, Eisenberg S, Breslow JL. Dietary polyunsaturated fats of the W-6 and W-3 series reduce postprandial lipoprotein levels. Chronic and acute effects of fat saturation on postprandial lipoprotein metabolism J Clin Invest 1988, 82(6), 1884-1893. 10.1172/JCI113806

[183] Zechner R, Newman TC, Sherry B, Cerami A, Breslow JL. Recombinant human cachectin/tumor necrosis factor but not interleukin-1 alpha downregulates lipoprotein lipase gene expression at the transcriptional level in mouse 3T3-L1 adipocytes. Mol Cell Biol 1988, 8(6), 2394-2401

[184] Desoye G, Schweditsch MO, Pfeiffer KP, Zechner R, Kostner GM. Correlation of hormones with lipid and lipoprotein levels during normal pregnancy and postpartum J Clin Endocrinol Metab 1987, 64(4), 704-712. 10.1210/jcem-64-4-704

[185] Kostner GM, Knipping G, Groener JE, Zechner R, Dieplinger H. The role of LCAT and cholesteryl ester transfer proteins for the HDL and LDL structure and metabolism Adv Exp Med Biol 1987, 21079-86

[186] Zechner R, Dieplinger H, Steyrer E, Groener J, Calvert D, Kostner GM. In vitro formation of HDL-2 from HDL-3 and triacylglycerol-rich lipoproteins by the action of lecithin:cholesterol acyltransferase and cholesterol ester transfer protein Biochim Biophys Acta 1987, 918(1), 27-35

[187] Knipping G, Birchbauer A, Steyrer E, Groener J, Zechner R, Kostner GM. Studies on the substrate specificity of human and pig lecithin: cholesterol acyltransferase: role of low-density lipoproteins Biochemistry 1986, 25(18), 5242-5249

[188] Zechner R, Desoye G, Schweditsch MO, Pfeiffer KP, Kostner GM. Fluctuations of plasma lipoprotein-A concentrations during pregnancy and post partum Metabolism 1986, 35(4), 333-336

[189] Zechner R, Moser R, Kostner GM. Isolation of pure LpB from human serum J Lipid Res 1986, 27(6), 681-686

[190] Dieplinger H, Zechner R, Kostner GM. The in vitro formation of HDL2 during the action of LCAT: the role of triglyceride-rich lipoproteins J Lipid Res 1985, 26(3), 273-282

[191] Druml W, Zechner R, Magometschnigg D, et al. Post-heparin lipolytic activity in acute renal failure Clin Nephrol 1985, 23(6), 289-293

[192] Knipping G, Zechner R, Kostner GM, Holasek A. Factors affecting the conversion of high-density lipoproteins: experiments with pig and human plasma Biochim Biophys Acta 1985, 835(2), 244-252

[193] Kostner GM, Hadorn B, Roscher A, Zechner R. Plasma lipids and lipoproteins of a patient with cholesteryl ester storage disease J Inherit Metab Dis 1985, 8(1), 9-12

[194] Kostner GM, Krempler F, Dieplinger H, Zechner R, Teubl I, Sandhofer F. Altered metabolism of low density lipoprotein in humans after prolonged incubation in plasma Clin Sci (Lond) 1985, 68(4), 411-418

[195] Kostner GM, Wurm H, Zechner R, Pilger E, Dieplinger H. Apolipoproteins: significance for lipid metabolism and for diagnosis of atherosclerotic disease Prog Clin Biol Res 1985, 18867-77

[196] Kostner GM, Zechner R, Bihari-Varga M, Gruber E. The interaction of human plasma low density lipoproteins with glycosamino-glycans: influence of the chemical composition Lipids 1985, 20(1), 24-28

[197] Knipping G, Steyrer E, Zechner R, Holasek A. Isolation and characterization of polymorphic forms of porcine apoC-II by chromatofocusing J Lipid Res 1984, 25(1), 86-91.

[198] Zechner R, Dieplinger H, Roscher A, Kostner GM. The low-density-lipoprotein pathway of native and chemically modified low-density lipoproteins isolated from plasma incubated in vitro Biochem J 1984, 224(2), 569-576

[199] Zechner R, Kostner GM, Dieplinger H, Degovics G, Laggner P. In vitro modification of the chemical composition of human plasma low density lipoproteins: effects of morphology and thermal properties Chem Phys Lipids 1984, 36(2), 111-119

[200] Prager R, Schernthaner G, Kostner GM, Mühlhauser I, Zechner R, Dorda W. Effect of bezafibrate on plasma lipids, lipoproteins, apolipoproteins AI, AII and B and LCAT

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activity in hyperlipidemic, non-insulin-dependent diabetics Atherosclerosis 1982, 43(2-3), 321-327

[201] Zechner R, Dieplinger H, Roscher A, Krempler F, Kostner GM. The role of lecithin:cholesterol acyltransferase for the low density lipoprotein composition and specific binding to the B receptor Biochim Biophys Acta 1982, 712(2), 433-435

Patents

Zimmermann R, Zechner R, Haemmerle G, Hoefler G, Das S, Lass A. 2015. Method for treatment of cachexia by administering inhibitors of adipose triglyceride lipase expression or activity, US 8993509 B2

Zechner R, Zimmerman R, Strauss J, Haemmerle G., Lass A. 2005. Pharmaceutical composition for modulating the activity of a novel triglyceride hydrolase. EP 1765997

Selected oral presentations

2015 Louis-Jeantet Prize Lecture at the EMBO Meeting Birmingham, UK, September 2015 2014 Richard Havel Lecture, Deuel Lipid Conference, San Diego, CA, USA, March 2014 2012 DeWitt S. Goodman Lectureship, Columbia University, NY, USA, sponsored by

Columbia University, New York, USA, July 2012 2012 Van Deenen Lecture - Lipolysis: how fat catabolism affects multiple aspects of lipid and

energy metabolism - at the International Conference on the Bioscience of Lipids and the American Society of Biochemistry and Molecular Biology, Banff, Canada, September 2012

2011 The Journal of Lipid Research Lectureship Award at the Kern Lipid Conference, Vail, Colorado, sponsored by the Journal of Lipid Research, January 2011

2009 European Lipid Science Award Lecture, sponsored by the European Federation for the Science and Technology of Lipids, October 2009

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Curriculum Vitae – Assoz. Prof. Dr. rer. nat. Robert Zimmermann Institute of Molecular Biosciences, University of Graz, Heinrichstrasse 31, 2nd floor, 8010 Graz, Austria, Phone: +43 (0) 316 380 1900(1904), E-Mail: [email protected] Year/ Place of Birth *1967/ Leibnitz, Austria Nationality Austria Orcid ID 0000-0002-7354-870X No of Publications 84 (22 first and/or corresponding) h-Index 37 (Scopus, Nov, 2016) Supervised Ph.D. Students since 2007: 8 finished, 3 ongoing Key words Lipid metabolism, lipid signaling, lipolysis, endocannobinoids,

lipases


since 2008 Associate Professor at the Institute of Molecular Biosciences, University of Graz, Austria

2008 Habilitation in Biochemistry and Molecular Biology, University of Graz, Austria

2002-2008 Assistant Professor at the Institute of Molecular Biosciences, University of Graz, Austria

1998-2002 Post-doc, Institute of Biochemistry, University of Graz, Austria Education

1998 Graduation “Dr. rer. nat.” 1994-1998 Ph.D. thesis at the Insitute of Medical Biochemistry, University of Graz,

Austria 1994 Graduation “Master of Science” 1992-1994 Diploma thesis at the Insitute of Medical Biochemistry, University of

Graz, Austria 1986-1992 Studies in Biology, University of Graz, Austria Externally funded national and international projects (since 2011)

Funding Org.

Project Number


Funding Period

FWF TRP4 Development of small molecule

inhibitors for ATGL 300 2010-2013

FWF P24294 Characterization of mice with tissue-specific deletion of monoglyceride

lipase 300 2012-2015

FWF P26166 Functional characterization of

α/β hydrolase domain-containing 6 (ABHD6)

450 2014-2017

FWF P 28533 Molecular mechanisms mediating zinc-alpha2-glycoprotein signaling

450 2016-2019


W9 DK Molecular Enzymology

Period 3/4 237 322

2011-2014 2014-2017


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Publications [1] Heier C, Xie H, Zimmermann R. Nonoxidative ethanol metabolism in humans-from

biomarkers to bioactive lipids IUBMB Life 2016, 68(12), 916-923. 10.1002/iub.1569 [2] Grumet L, Eichmann TO, Taschler U, et al. Lysosomal Acid Lipase Hydrolyzes Retinyl

Ester and Affects Retinoid Turnover J Biol Chem 2016, 291(34), 17977-17987. 10.1074/jbc.M116.724054

[3] Al-Zoughbi W, Pichler M, Gorkiewicz G, et al. Loss of adipose triglyceride lipase is associated with human cancer and induces mouse pulmonary neoplasia Oncotarget 2016, 7(23), 33832-33840. 10.18632/oncotarget.9418

[4] Vujic N, Schlager S, Eichmann TO, et al. Monoglyceride lipase deficiency modulates endocannabinoid signaling and improves plaque stability in ApoE-knockout mice Atherosclerosis 2016, 2449-21. 10.1016/j.atherosclerosis.2015.10.109

[5] Heier C, Taschler U, Radulovic M, et al. Monoacylglycerol Lipases Act as Evolutionarily Conserved Regulators of Non-oxidative Ethanol Metabolism J Biol Chem 2016, 291(22), 11865-11875. 10.1074/jbc.M115.705541

[6] Aschauer P, Rengachari S, Lichtenegger J, et al. Crystal structure of the Saccharomyces cerevisiae monoglyceride lipase Yju3p Biochim Biophys Acta 2016, 1861(5), 462-470. 10.1016/j.bbalip.2016.02.005

[7] Doler C, Schweiger M, Zimmermann R, Breinbauer R. Chemical Genetic Approaches for the Investigation of Neutral Lipid Metabolism Chembiochem 2016, 17(5), 358-377. 10.1002/cbic.201500501

[8] Grabner GF, Eichmann TO, Wagner B, et al. Deletion of Monoglyceride Lipase in Astrocytes Attenuates Lipopolysaccharide-Induced Neuroinflammation J Biol Chem 2015, 291(2), 913-923. 10.1074/jbc.M115.683615

[9] Pribasnig MA, Mrak I, Grabner GF, et al. α/β Hydrolase Domain-containing 6 (ABHD6) Degrades the Late Endosomal/Lysosomal Lipid Bis(monoacylglycero)phosphate J Biol Chem 2015, 290(50), 29869-29881. 10.1074/jbc.M115.669168

[10] Schreiber R, Hofer P, Taschler U, et al. Hypophagia and metabolic adaptations in mice with defective ATGL-mediated lipolysis cause resistance to HFD-induced obesity Proc Natl Acad Sci U S A 2015, 112(45), 13850-13855. 10.1073/pnas.1516004112

[11] Heier C, Radner FPW, Moustafa T, et al. G0/G1 Switch Gene 2 Regulates Cardiac Lipolysis J Biol Chem 2015, 290(43), 26141-26150. 10.1074/jbc.M115.671842

[12] Wang H, Bell M, Sreenivasan U, et al. Unique regulation of adipose triglyceride lipase (ATGL) by perilipin 5, a lipid droplet-associated protein J Biol Chem 2011, 286(18), 15707-15715. M110.207779 [pii]10.1074/jbc.M110.207779

[13] Eichmann TO, Grumet L, Taschler U, et al. ATGL and CGI-58 are lipid droplet proteins of the hepatic stellate cell line HSC-T6 J Lipid Res 2015, 56(10), 1972-1984. 10.1194/jlr.M062372

[14] Taschler U, Eichmann TO, Radner FPW, et al. Monoglyceride lipase deficiency causes desensitization of intestinal cannabinoid receptor type 1 and increased colonic μ-opioid receptor sensitivity Br J Pharmacol 2015, 172(17), 4419-4429. 10.1111/bph.13224

[15] Hofer P, Boeszoermenyi A, Jaeger D, et al. Fatty Acid-binding Proteins Interact with Comparative Gene Identification-58 Linking Lipolysis with Lipid Ligand Shuttling J Biol Chem 2015, 290(30), 18438-18453. 10.1074/jbc.M114.628958

[16] Taschler U, Schreiber R, Chitraju C, et al. Adipose triglyceride lipase is involved in the mobilization of triglyceride and retinoid stores of hepatic stellate cells Biochim Biophys Acta 2015, 1851(7), 937-945. 10.1016/j.bbalip.2015.02.017

[17] Navia-Paldanius D, Aaltonen N, Lehtonen M, et al. Increased tonic cannabinoid CB1R activity and brain region-specific desensitization of CB1R Gi/o signaling axis in mice with global genetic knockout of monoacylglycerol lipase Eur J Pharm Sci 2015, 77180-188. 10.1016/j.ejps.2015.06.005

[18] Mayer N, Schweiger M, Melcher M-C, et al. Structure-activity studies in the development of a hydrazone based inhibitor of adipose-triglyceride lipase (ATGL) Bioorg Med Chem 2015, 23(12), 2904-2916. 10.1016/j.bmc.2015.02.051

[19] Pollak NM, Jaeger D, Kolleritsch S, et al. The interplay of protein kinase A and perilipin 5 regulates cardiac lipolysis J Biol Chem 2015, 290(3), 1295-1306.

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10.1074/jbc.M114.604744 [20] Heier C, Zimmermann R. The sparing use of fat: G0s2 controls lipolysis and fatty acid

oxidation Diabetologia 2015, 58(1), 7-9. 10.1007/s00125-014-3430-6 [21] Cerk IK, Salzburger B, Boeszoermenyi A, et al. A peptide derived from G0/G1 switch gene

2 acts as noncompetitive inhibitor of adipose triglyceride lipase J Biol Chem 2014, 289(47), 32559-32570. 10.1074/jbc.M114.602599

[22] Nagy HM, Paar M, Heier C, et al. Adipose triglyceride lipase activity is inhibited by long-chain acyl-coenzyme A Biochim Biophys Acta 2014, 1841(4), 588-594. 10.1016/j.bbalip.2014.01.005

[23] Jha P, Claudel T, Baghdasaryan A, et al. Role of adipose triglyceride lipase (PNPLA2) in protection from hepatic inflammation in mouse models of steatohepatitis and endotoxemia Hepatology 2014, 59(3), 858-869. 10.1002/hep.26732

[24] Schweiger M, Eichmann TO, Taschler U, Zimmermann R, Zechner R, Lass A. Measurement of lipolysis Methods Enzymol 2014, 538171-193. 10.1016/B978-0-12-800280-3.00010-4

[25] Mayer N, Schweiger M, Romauch M, et al. Development of small-molecule inhibitors targeting adipose triglyceride lipase Nat Chem Biol 2013, 9(12), 785-787. 10.1038/nchembio.1359

[26] Thomas G, Betters JL, Lord CC, et al. The Serine Hydrolase ABHD6 Is a Critical Regulator of the Metabolic Syndrome Cell Rep 2013. S2211-1247(13)00507-X [pii]10.1016/j.celrep.2013.08.047

[27] Alsted TJ, Ploug T, Prats C, et al. Contraction-induced lipolysis is not impaired by inhibition of hormone-sensitive lipase in skeletal muscle J Physiol 2013, 591(Pt 20), 5141-5155. jphysiol.2013.260794 [pii]10.1113/jphysiol.2013.260794

[28] Janssen MCH, van Engelen B, Kapusta L, et al. Symptomatic lipid storage in carriers for the PNPLA2 gene Eur J Hum Genet 2013, 21(8), 807-815. 10.1038/ejhg.2012.256

[29] Zierler KA, Jaeger D, Pollak NM, et al. Functional cardiac lipolysis in mice critically depends on comparative gene identification-58 J Biol Chem 2013, 288(14), 9892-9904. M112.420620 [pii]10.1074/jbc.M112.420620

[30] Wang H, Sreenivasan U, Gong DW, et al. Cardiomyocyte-specific perilipin 5 overexpression leads to myocardial steatosis and modest cardiac dysfunction J Lipid Res 2013, 54(4), 953-965. jlr.M032466 [pii]10.1194/jlr.M032466

[31] Pollak NM, Schweiger M, Jaeger D, et al. Cardiac-specific overexpression of perilipin 5 provokes severe cardiac steatosis via the formation of a lipolytic barrier J Lipid Res 2013, 54(4), 1092-1102. jlr.M034710 [pii]10.1194/jlr.M034710

[32] Eichmann TO, Kumari M, Haas JT, et al. Studies on the substrate and stereo/regioselectivity of adipose triglyceride lipase, hormone-sensitive lipase, and diacylglycerol-O-acyltransferases J Biol Chem 2012, 287(49), 41446-41457. M112.400416 [pii]10.1074/jbc.M112.400416

[33] Schweiger M, Paar M, Eder C, et al. G0/G1 switch gene-2 regulates human adipocyte lipolysis by affecting activity and localization of adipose triglyceride lipase J Lipid Res 2012, 53(11), 2307-2317. 10.1194/jlr.M027409

[34] Chitraju C, Trötzmüller M, Hartler J, et al. Lipidomic analysis of lipid droplets from murine hepatocytes reveals distinct signatures for nutritional stress J Lipid Res 2012, 53(10), 2141-2152. 10.1194/jlr.M028902

[35] Rengachari S, Bezerra GA, Riegler-Berket L, et al. The structure of monoacylglycerol lipase from Bacillus sp. H257 reveals unexpected conservation of the cap architecture between bacterial and human enzymes Biochim Biophys Acta 2012, 1821(7), 1012-1021. 10.1016/j.bbalip.2012.04.006

[36] Kumari M, Schoiswohl G, Chitraju C, et al. Adiponutrin functions as a nutritionally regulated lysophosphatidic acid acyltransferase Cell Metab 2012, 15(5), 691-702. S1550-4131(12)00148-9 [pii]10.1016/j.cmet.2012.04.008

[37] Paar M, Jungst C, Steiner NA, et al. Remodeling of lipid droplets during lipolysis and growth in adipocytes J Biol Chem 2012, 287(14), 11164-11173. M111.316794 [pii]10.1074/jbc.M111.316794

[38] Zechner R, Zimmermann R, Eichmann TO, et al. FAT SIGNALS--lipases and lipolysis in

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lipid metabolism and signaling Cell Metab 2012, 15(3), 279-291. S1550-4131(12)00018-6 [pii]10.1016/j.cmet.2011.12.018

[39] Grall A, Guaguère E, Planchais S, et al. PNPLA1 mutations cause autosomal recessive congenital ichthyosis in golden retriever dogs and humans Nat Genet 2012, 44(2), 140-147. 10.1038/ng.1056

[40] Schreiber R, Taschler U, Preiss-Landl K, Wongsiriroj N, Zimmermann R, Lass A. Retinyl ester hydrolases and their roles in vitamin A homeostasis Biochim Biophys Acta 2012, 1821(1), 113-123. S1388-1981(11)00068-0 [pii]10.1016/j.bbalip.2011.05.001

[41] Etschmaier K, Becker T, Eichmann TO, et al. Adipose triglyceride lipase affects triacylglycerol metabolism at brain barriers J Neurochem 2011, 119(5), 1016-1028. 10.1111/j.1471-4159.2011.07498.x

[42] Cornaciu I, Boeszoermenyi A, Lindermuth H, et al. The minimal domain of adipose triglyceride lipase (ATGL) ranges until leucine 254 and can be activated and inhibited by CGI-58 and G0S2, respectively PLoS One 2011, 6(10), e26349. 10.1371/journal.pone.0026349PONE-D-11-15986 [pii]

[43] Haemmerle G, Moustafa T, Woelkart G, et al. ATGL-mediated fat catabolism regulates cardiac mitochondrial function via PPAR-alpha and PGC-1 Nat Med 2011, 17(9), 1076-1085. nm.2439 [pii]10.1038/nm.2439

[44] Das SK, Eder S, Schauer S, et al. Adipose triglyceride lipase contributes to cancer-associated cachexia Science (80- ) 2011, 333(6039), 233-238. science.1198973 [pii]10.1126/science.1198973

[45] Taschler U, Radner FPW, Heier C, et al. Monoglyceride lipase deficiency in mice impairs lipolysis and attenuates diet-induced insulin resistance J Biol Chem 2011, 286(20), 17467-17477. 10.1074/jbc.M110.215434

[46] Lass A, Zimmermann R, Oberer M, Zechner R. Lipolysis - a highly regulated multi-enzyme complex mediates the catabolism of cellular fat stores Prog Lipid Res 2011, 50(1), 14-27. S0163-7827(10)00052-4 [pii]10.1016/j.plipres.2010.10.004

[47] Coassin S, Schweiger M, Kloss-Brandstatter A, et al. Investigation and functional characterization of rare genetic variants in the adipose triglyceride lipase in a large healthy working population PLoS Genet 2010, 6(12), e1001239. 10.1371/journal.pgen.1001239

[48] Molhoj S, Hansen HS, Schweiger M, Zimmermann R, Johansen T, Malmlof K. Effect of the cannabinoid receptor-1 antagonist rimonabant on lipolysis in rats Eur J Pharmacol 2010, 646(1-3), 38-45. S0014-2999(10)00791-0 [pii]10.1016/j.ejphar.2010.08.006

[49] Buchebner M, Pfeifer T, Rathke N, et al. Cholesteryl ester hydrolase activity is abolished in HSL-/- macrophages but unchanged in macrophages lacking KIAA1363 J Lipid Res 2010, 51(10), 2896-2908. jlr.M004259 [pii]10.1194/jlr.M004259

[50] Heier C, Taschler U, Rengachari S, et al. Identification of Yju3p as functional orthologue of mammalian monoglyceride lipase in the yeast Saccharomycescerevisiae Biochim Biophys Acta 2010, 1801(9), 1063-1071. S1388-1981(10)00118-6 [pii]10.1016/j.bbalip.2010.06.001

[51] Gruber A, Cornaciu I, Lass A, et al. The N-terminal Region of Comparative Gene Identification-58 (CGI-58) Is Important for Lipid Droplet Binding and Activation of Adipose Triglyceride Lipase J Biol Chem 2010, 285(16), 12289-12298. 10.1074/jbc.M109.064469

[52] Radner FPW, Streith IE, Schoiswohl G, et al. Growth retardation, impaired triacylglycerol catabolism, hepatic steatosis, and lethal skin barrier defect in mice lacking comparative gene identification-58 (CGI-58) J Biol Chem 2010, 285(10), 7300-7311. 10.1074/jbc.M109.081877

[53] Schoiswohl G, Schweiger M, Schreiber R, et al. Adipose triglyceride lipase plays a key role in the supply of the working muscle with fatty acids J Lipid Res 2010, 51(3), 490-499. 10.1194/jlr.M001073

[54] Schreiber R, Taschler U, Wolinski H, et al. Esterase 22 and beta-glucuronidase hydrolyze retinoids in mouse liver J Lipid Res 2009, 50(12), 2514-2523. 10.1194/jlr.M000950

[55] Schweiger M, Lass A, Zimmermann R, Eichmann TO, Zechner R. Neutral lipid storage disease: genetic disorders caused by mutations in adipose triglyceride lipase/PNPLA2 or CGI-58/ABHD5 Am J Physiol Endocrinol Metab 2009, 297(2), E289-96. 00099.2009 [pii]10.1152/ajpendo.00099.2009

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[56] Zechner R, Kienesberger PC, Haemmerle G, Zimmermann R, Lass A. Adipose triglyceride lipase and the lipolytic catabolism of cellular fat stores J Lipid Res 2009, 50(1), 3-21. R800031-JLR200 [pii]10.1194/jlr.R800031-JLR200

[57] Alsted TJ, Nybo L, Schweiger M, et al. Adipose triglyceride lipase in human skeletal muscle is upregulated by exercise training Am J Physiol Endocrinol Metab 2009, 296(3), E445-53. 90912.2008 [pii]10.1152/ajpendo.90912.2008

[58] Schweiger M, Schoiswohl G, Lass A, et al. The C-terminal region of human adipose triglyceride lipase affects enzyme activity and lipid droplet binding J Biol Chem 2008, 283(25), 17211-17220. 10.1074/jbc.M710566200

[59] Kienesberger PC, Lass A, Preiss-Landl K, et al. Identification of an insulin-regulated lysophospholipase with homology to neuropathy target esterase J Biol Chem 2008, 283(9), 5908-5917. M709598200 [pii]10.1074/jbc.M709598200

[60] Schweiger M, Schreiber R, Haemmerle G, et al. Adipose triglyceride lipase and hormone-sensitive lipase are the major enzymes in adipose tissue triacylglycerol catabolism J Biol Chem 2006, 281(52), 40236-40241. 10.1074/jbc.M608048200

[61] Tschernatsch MM, Mlecnik B, Trajanoski Z, Zechner R, Zimmermann R. LPL-mediated lipolysis of VLDL induces an upregulation of AU-rich mRNAs and an activation of HuR in endothelial cells Atherosclerosis 2006, 189(2), 310-317. S0021-9150(06)00033-5 [pii]10.1016/j.atherosclerosis.2006.01.007

[62] Schoenborn V, Heid IM, Vollmert C, et al. The ATGL gene is associated with free fatty acids, triglycerides, and type 2 diabetes Diabetes 2006, 55(5), 1270-1275.

[63] Heckmann BL, Zhang X, Xie X, et al. Defective adipose lipolysis and altered global energy metabolism in mice with adipose overexpression of the lipolytic inhibitor G0/G1 switch gene 2 (G0S2) J Biol Chem 2014, 289(4), 1905-1916. 10.1074/jbc.M113.522011

[64] Lass A, Zimmermann R, Haemmerle G, et al. Adipose triglyceride lipase-mediated lipolysis of cellular fat stores is activated by CGI-58 and defective in Chanarin-Dorfman Syndrome Cell Metab 2006, 3(5), 309-319. S1550-4131(06)00114-8 [pii]10.1016/j.cmet.2006.03.005

[65] Kurat CF, Natter K, Petschnigg J, et al. Obese yeast: triglyceride lipolysis is functionally conserved from mammals to yeast J Biol Chem 2006, 281(1), 491-500. M508414200 [pii]10.1074/jbc.M508414200

[66] Birner-Gruenberger R, Susani-Etzerodt H, Waldhuber M, et al. The lipolytic proteome of mouse adipose tissue Mol Cell Proteomics 2005, 4(11), 1710-1717. 10.1074/mcp.M500062-MCP200

[67] Jandrositz A, Petschnigg J, Zimmermann R, et al. The lipid droplet enzyme Tgl1p hydrolyzes both steryl esters and triglycerides in the yeast, Saccharomyces cerevisiae Biochim Biophys Acta 2005, 1735(1), 50-58. 10.1016/j.bbalip.2005.04.005

[68] Zechner R, Strauss JG, Haemmerle G, Lass A, Zimmermann R. Lipolysis: pathway under construction Curr Opin Lipidol 2005, 16(3), 333-340. 00041433-200506000-00011 [pii]

[69] Kratky D, Zimmermann R, Wagner EM, et al. Endothelial lipase provides an alternative pathway for FFA uptake in lipoprotein lipase-deficient mouse adipose tissue J Clin Invest 2005, 115(1), 161-167. 10.1172/JCI15972

[70] Zimmermann R, Strauss JG, Haemmerle G, et al. Fat mobilization in adipose tissue is promoted by adipose triglyceride lipase Science (80- ) 2004, 306(5700), 1383-1386. 306/5700/1383 [pii]10.1126/science.1100747

[71] Marsche G, Zimmermann R, Horiuchi S, Tandon NN, Sattler W, Malle E. Class B scavenger receptors CD36 and SR-BI are receptors for hypochlorite-modified low density lipoprotein J Biol Chem 2003, 278(48), 47562-47570. 10.1074/jbc.M308428200

[72] Zimmermann R, Haemmerle G, Wagner EM, Strauss JG, Kratky D, Zechner R. Decreased fatty acid esterification compensates for the reduced lipolytic activity in hormone-sensitive lipase-deficient white adipose tissue J Lipid Res 2003, 44(11), 2089-2099. 10.1194/jlr.M300190-JLR200

[73] Voshol PJ, Haemmerle G, Ouwens DM, et al. Increased hepatic insulin sensitivity together with decreased hepatic triglyceride stores in hormone-sensitive lipase-deficient mice Endocrinology 2003, 144(8), 3456-3462. 10.1210/en.2002-0036

[74] Haemmerle G, Zimmermann R, Zechner R. Letting lipids go: hormone-sensitive lipase

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Curr Opin Lipidol 2003, 14(3), 289-297. 10.1097/01.mol.0000073509.41685.36 [75] Strauss JG, Zimmermann R, Hrzenjak A, et al. Endothelial cell-derived lipase mediates

uptake and binding of high-density lipoprotein (HDL) particles and the selective uptake of HDL-associated cholesterol esters independent of its enzymic activity Biochem J 2002, 368(Pt 1), 69-79. 10.1042/BJ20020306BJ20020306 [pii]

[76] Preiss-Landl K, Zimmermann R, Hammerle G, Zechner R. Lipoprotein lipase: the regulation of tissue specific expression and its role in lipid and energy metabolism Curr Opin Lipidol 2002, 13(5), 471-481.

[77] Haemmerle G, Zimmermann R, Hayn M, et al. Hormone-sensitive lipase deficiency in mice causes diglyceride accumulation in adipose tissue, muscle, and testis J Biol Chem 2002, 277(7), 4806-4815. 10.1074/jbc.M110355200M110355200 [pii]

[78] Haemmerle G, Zimmermann R, Strauss JG, et al. Hormone-sensitive lipase deficiency in mice changes the plasma lipid profile by affecting the tissue-specific expression pattern of lipoprotein lipase in adipose tissue and muscle J Biol Chem 2002, 277(15), 12946-12952. 10.1074/jbc.M108640200M108640200 [pii]

[79] Zimmermann R, Panzenbock U, Wintersperger A, et al. Lipoprotein lipase mediates the uptake of glycated LDL in fibroblasts, endothelial cells, and macrophages Diabetes 2001, 50(7), 1643-1653.

[80] Van Eck M, Zimmermann R, Groot PH, Zechner R, Van Berkel TJ. Role of macrophage-derived lipoprotein lipase in lipoprotein metabolism and atherosclerosis Arter Thromb Vasc Biol 2000, 20(9), E53-62.

[81] Zimmermann R, Sartipy P, Winkler R, Zechner R, Hurt-Camejo E, Kostner GM. Endogenously produced glycosaminoglycans affecting the release of lipoprotein lipase from macrophages and the interaction with lipoproteins Biochim Biophys Acta 2000, 1484(2-3), 316-324.

[82] Wang X, Greilberger J, Levak-Frank S, Zimmermann R, Zechner R, Jürgens G. Endogenously produced lipoprotein lipase enhances the binding and cell association of native, mildly oxidized and moderately oxidized low-density lipoprotein in mouse peritoneal macrophages Biochem J 1999, 343 Pt 2347-353.

[83] Kostner GM, Wo X, Frank S, Kostner K, Zimmermann R, Steyrer E. Metabolism of Lp(a): assembly and excretion Clin Genet 1997, 52(5), 347-354.

[84] Panzenboeck U, Wintersberger A, Levak-Frank S, et al. Implications of endogenous and exogenous lipoprotein lipase for the selective uptake of HDL3-associated cholesteryl esters by mouse peritoneal macrophages J Lipid Res 1997, 38(2), 239-253.

Patents

Zimmermann R, Zechner R, Haemmerle G, Hoefler G, Das S, Lass A. 2015. Method for treatment of cachexia by administering inhibitors of adipose triglyceride lipase expression or activity, US 8993509 B2

Schweiger M, Romauch M, Zimmermann R, Mayer N, Breinbauer R. 2015. ATGListatin and pharmaceutical composition comprising the same, US9206115 B2:

Zimmermann R, Zechner R, Hämmerle G, Höfler G, Das S, Lass A. 2010. Modulation of adipose triglyderide lipase for prevention and treatment of cachexia, loss of weight and muscle atrophy and methods of screening therefor, US 13/262,094

Zechner R, Zimmerman R, Strauss J, Haemmerle G., Lass A. 2005. Pharmaceutical composition for modulating the activity of a novel triglyceride hydrolase. EP 1765997

research grants of the consortium members

69

Patents of the consortium members (complete list)

Authors Patent Information Year

Roier S, Reidl J, Schild S. EP 2505208: Vaccine against Pasteurellaceae. 2012

Schild S, Nelson EJ, Camilli A. WO2009049013 A2: Cholera Vaccines. 2008

Wanker EE, Göhler M, Lalowski H, Stelzl U, Lehrach H, Strödicke M.

EP03013957.0: Disease Related Protein Network. 2003

Wanker EE, Stelzl U, Lalowski H, Göhler M, Worm U, Hänig C, Strödicke M.

EP04030994.0: Method for the identification of protein-protein interactions in a disease relevant protein network.

2005

Zechner R, Zimmerman R, Strauss J, Hämmerle G.

EP 250050745594 Modulating the activity of triglyceride hydrolase

2005

Zimmermann R, Zechner R, Hämmerle G, Höfler G, Das S, Lass A

US 13/262,094, Modulation of adipose triglyderide lipase for prevention and treatment of cachexia, loss of weight and muscle atrophy and methods of screening therefor

2010

Schweiger M, Romauch M, Zimmermann R, Mayer N, Breinbauer R

US9206115 B2: ATGListatin and pharmaceutical composition comprising the same

2015

Schweiger M, Romauch M, Zimmermann R, Mayer N, Fuchs E, Breinbauer R.

WO2014114649 A: Lipase inhibitors. 2014

Baekelandt V, Buettner S, Madeo F, Winderickx J

US2014093494 (A1): Alpha Synuclein Toxicity 2014

Winderickx J, Van den Berghe G, Gunst J, Vanhorebeek I, Langouche L, Eisenberg T, Madeo F, Magnes C, Sinner F

US2012015901 (A1): Methods and Preparations for Protecting Critically Ill Patients

2012

Madeo F, Eisenberg T, Pieber T, Magnes C, Sinner F, Winderickx J, Van den Berghe G

WO2012007531 (A2); WO2012007531 (A3): Methods and Compostions for Diagnosing Medical Conditions

2012

Eisenberg T, Rockenfeller P, Madeo F, Minois N, Kroemer G, Winderickx J

NL1036427 (C): Activators of the Autophagic Pathway

2010

Baekelandt V, Buettner S, Gerard M, Madeo F, Windericks J

WO2010094090 (A3); WO2010094090 (A2): Synucleinophaties

2010

Winderickx J, Eisenberg T, Kroemer G, Madeo F, Minois N, Rockenfeller P

WO2010081204 (A2); WO2010081204 (A3): Activators of the Autophagic Pathway

2010

Froehlich KU, Madeo F DE19842612 (A1): Rapid Screening Assay for Apoptosis-modulating Substances Comprises Determining the Effects of Substances on Yeasts

2000


70

Research grants of the consortium members (since 2011)

PI Funding Org. Project

Number Research Topic

Amount Funded [kEuro]

Funding Period

Schild, Zechner E.

BioTechMed-Graz

SECRETOME

Immunomodulatory and Antiproliferative Bioactivities

of the Bacterial Secretome in Gastrointestinal Disease

and Therapy

429 2017-2019

Madeo, Oberer,

Zechner R.

FWF/ University Support

F30 SFB LIPOTOX - 3rd Funding

Period

MO: 236 FM: 214 RZ: 660

2014-2017

Oberer Schild,

Zangger Zechner E., Zechner R.,

Zimmermann


W901 DK Molecular Enzymology, funding period 4, (Director:

Zechner E.) 322 / PI

2014-2017

Oberer, Zechner R.

Foundation Leducq

12 CVD 04

Transatlantic Network on Triglycerides - Triglyceride Metabolism in Obesity and


881 2013-2017

Madeo, Oberer,

Zechner R.


F30 SFB LIPOTOX - 2nd Funding

Period

MO: 340 FM: 465 RZ: 1.67

2011-2014

Oberer Schild,


Zimmermann




2011-2014

Oberer Schild,


Zimmermann




2008-2011

Gorkiewicz Zechner E.

FWF P20479 Comparative Analysis of

Campylobacter fetus virulence mechanisms

234 2008-2013

Madeo, Zechner R.


F30 SFB LIPOTOX – 1st Funding

Period FM: 431 RZ: 1.49

2007-2011

Kühnlein DFG KU 2866/1-1

Regulation of Drosophila melanogaster body fat

content by store-operated calcium entry

133 2013-2016

Kühnlein Behrens Weise

Foundation -

Nutrigenomics of Obesity using the Drosophila Model

Organism 150

2013-2015

Kühnlein Max Planck

Society -

Molecular Physiology of Drosophila

700 2010-2016


71

Madeo FWF P29203 Causal epigenetic changes

during aging and caloric restriction

352 2016-2019

Madeo FWF P29262 Mechanisms of Spermidine mediated neuroprotection

339 2016-2019

Madeo FWF P27893 Pro-autophagic Polyphenols

and Polyamines for Longevity 453

2015-2018

Madeo FWF P24381 Causes of spermidine-

mediated longevity 395

2012-2015

Madeo University

support -

Unconventional research programm

420 2014-2018

Madeo University Support

- FlySleep 184 2014-2017

Madeo FWF/DFG “DACH”

I1000 Linking mitochondrial

dynamics with neurotoxicity in yeast cell death models

144 2012-2015

Madeo FWF P23490 Autophagic cell death:

characteristics and regulation 376

2011-2014

Madeo FWF W1226 DK Metabolic and

Cardiovascular Disease 392

2010-2018

Oberer University of

Graz N/A

36 Incentive Förderung: S-Layer Proteins

36 2016

Oberer Foundation

Leducq 12CVD04

Transatlantic Network on Triglycerides — Triglyceride Metabolism in Obesity and


191 2013-2017

Oberer FWF P24857 Structure, dynamics, and

substrate specificity of monoglyceride lipases

348 2012-2017

Oberer NAWI Graz N/A

Unraveling the secrets of the “lipolysome”:

Characterization of lipolytic proteins and its protein-

protein complexes

90 2012-2015

Oberer FWF P22170 Functional Characterization of

CGI-58 293

2010-2015

Schild FWF P 27654 Characterization of Vibrio

cholerae biofilms 329

2016 - 2021

Schild FWF P 25691 Outer membrane vesicles of

enteric pathogens 306

2013-2018

Schild FWF P 22986 Gene regulation in dynamic

biofilms 282

2011-2015

Stelzl Max Planck

Society -

The human RNA-binding protein interactome

78 2015

Stelzl BMBF (DE) 01GS08171 NGFNplus 500 2008–2011

Stelzl Helmholtz

Society - Alliance on Systems Biology 95

2008–2010

Stelzl Max Planck

Society -

Molecular Interaction Networks

2900 2007–2016


72

Zangger FWF P27793 Spatially-selective NMR

spectroscopy 273

2015-2017

Zangger FWF P25880 NMR of paramagnetic

relaxation enhancements 231

2013-2016

Zangger FWF P24742 Pure shift NMR 276 2012-2015

Zangger Austrian

Ministry of Science

University Infra-

structure

700 MHz liquid state-NMR spectrometer

1700 2011

Zangger FWF P22630 NMR studies on the

relaxosome 272

2010-2013

Zangger FWF P20020 Membrane-bound toxin-

antitoxin systems 271

2007-2011

Zechner E. University Support

- Bacterial communication and

competition in the human intestine

193 2016-2019

Zechner E. BioTechMed-

Graz -

Bacterial metabolite signal molecules in the intestinal

microbiota 155

2014-2017

Zechner E. FWF P24016 Regulation of the initiation of

bacterial type IV secretion 326

2011-2016

Zechner E. EU EFRE /

County of Styria -

Bacterial type IV protein translocation and Catheter-

associated urinary tract infections

310 2011-2014

Zechner E. FWF P21434

Genetic analysis of bacterial biofilm formation and migration on urethral

catheters (co-PI: A. Reisner)

276 2009-2012

Zechner R ERC ERC-2013-

ADG 340896

ERC Advanced Grant: The Role of Lipolysis in the

Pathogenesis of Cancer-associated Cachexia

2500 2014-2019

Zechner R Louise Jeantet

Prize -

Lipolysis – Characterization of Crucial Enzymes and (Patho)Physiological

Mechanisms

605 2015-2019

Zechner R FWF Z136 Wittgenstein Award:

Metabolic Lipase in Energy and Lipid Metabolism

1500 2008-2014

Zechner R

GEN-AU - Genome

Research in Austria/FFG

FFG-820979

GOLD I-III - Genomics Of Lipid-associated Disorders

(Coordinator & PI)

3000 (11 Mio

for consortiu

m)

2002-2012

Zimmermann FWF P26166 Functional characterization of

α/β hydrolase domain-containing 6 (ABHD6)

450 2014-2017

Zimmermann FWF P24294 Characterization of mice with

tissue-specific deletion of monoglyceride lipase

300 2012-2015


73

Zimmermann FWF TRP4 Development of small

molecule inhibitors for ATGL 300

2010-2013

Zimmermann FWF P21296 Lipases in monoacylglycerol and retinylester metabolism

400 2009-2012

Zimmermann FFG/BMWF GEN-AU GOLD III

Molecular mechanisms regulating lipolysis (C7)

180 2009-2012

Prizes and awards of the consortium members

74

Prizes and awards of the consortium members (since 2011)

PI Prize/ Award Year

Madeo Erzherzog-Johann Research Award 2015

Oberer Promotion Award for Scientific Achievements (Förderungungspreis) sponsored by the State of Styria, Austria

2015

Oberer Journal of Structural and Functional Genomics Poster Award, ICSG, Rehovot, Israel

2015

Oberer Kardinal-Innitzer-Foerderungspreis (Cardinal-Innitzer Promotion Award), Kardinal-Innitzer-Studienfonds, Vienna

2013

Schild Innovation Award, Life Science Park Styria, Graz 2014

Schild Travel award from the Land Steiermark 2011

Schild, Zechner E.

Wewalka Prize 2015, Salzburg + Top Abstract Prize 2014, United European Gastroenterologists, Berlin for the publication: Schneditz G, Rentner J, Roier S, Herzog KAT, Bücker R, Tröger H, Schild S, Weber H, Breinbauer R, Gorkiewicz G, Högenauer C, Zechner EL. 2014. Enterotoxicity of a nonribosomal peptide causes antibiotic-associated colitis. PNAS. 111(36):13181-13186.

2015 and 2014

Zechner R. Louis-Jeantet Prize for Medicine 2015

Zechner R. Richard Havel Award Lecture at The Deuel Lipid Conference, San Diego, CA, USA.

2014

Zechner R. Science Award, sponsored by the Country of Styria 2013

Zechner R. ERC Advanced Grant by the European Research Council 2013

Zechner R Kardinal-Innitzer-Würdigungspreis, sponsored by the Kardinal-Innitzer Studienfonds, Austria

2012

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