structure of macromolecules …by small and simple things are great things brought to pass
TRANSCRIPT
Structure of Structure of MacromoleculMacromolecul
eses
…by small and simple things are great things
brought to pass.
Macromolecules
Macromolecules are polymers constructed by the formation of covalent bonds between
smaller molecules called monomers
Molecular weight exceeding 1000
Condensation and Hydrolysis
Monomers are joined by condensation reactions, which
release a molecule of water for each bond
formed.
Hydrolysis reactions use water to break
polymers into monomers.
Molecular organization in the cell is a hierarchy
4 BASIC BIOLOGICAL MACROMOLECULES
• Proteins
• Carbohydrates
• Lipids
• Nucleic acids
Proteins
Proteins are polymers composed of hundreds or even thousands of amino acids linked in series by peptide bonds.
Basic building block is the amino acid
The functions of proteins include support, protection, catalysis, transport, defense, regulation, and movement.
What is amino acid?
• Amino means?
• Acid?
• There are 20 amino acids commonly
found in proteins
• peptide linkages form by condensation reactions between the carboxyl and amino groups of adjacent amino acids.
Amino Acids1. Alanine
2. Arginine
3. Lysine
4. Glycine
5. Asparagine
6. Methionine
7. Isoleucine
8. Aspartic acid
9. Tryptophan
10.Leucine
11.Cysteine
12.Tyrosine
13.Phenylalanine
14. Glutamic acid
15. Threonine
16. Proline
17. Valine
18. Histidine
19. Glutamine
20. Serine
Proteins: Structure
• Primary Structure
• Secondary Structure
• Tertiary Structure
• Quaternary Structure
Proteins: Structure
Primary Structure: the sequence of amino acids bonded by peptide linkages (Diversity
20n) (covalent bonding)
Even a slight change in the amino acid sequence can
cause the protein to malfunction
For example, A single amino acid substitution in hemoglobin causes sickle cell disease
Secondary Structure Results from hydrogen bonding between the oxygen of one amino acid and the hydrogen of
another(non covalent interactions)
α helices and β pleated sheets (maintained by hydrogen bonds between atoms of the amino acid residues)
The alpha helix is a coiled secondary structure due to a
hydrogen bond every fourth amino
acid
The beta pleated sheet is formed by hydrogen bonds between parallel
parts of the protein
A single polypeptide may have portions with both types of
secondary structure
Generated by bending and folding of the polypeptide chain
1) Covalent disulfide bridges, 2)Hydrophobic interactions 3) van der Waals forces 4) Ionic bonds
Tertiary Structure
Quaternary structure results from interactions among
separate polypeptide chains.
Proteins: Denaturation
A loss of three-dimensional structure sufficient to
cause loss of function is called denaturation. Proteins are denatured by heat, alterations in pH, or certain
chemicals lose their tertiary and secondary structure as well as their biological function. Renaturation is
not often possible.
Carbohydrates
Carbohydrates are polyhydroxy aldehydes or ketones,
or substances that yield such compounds on hydrolysis
They act as source of energy that can be transported
They also have structural roles
classification
• Monosaccharides
• Disaccharides
• Oligosaccharides (3-20)
• Polysaccharides
Monosaccharides
The monosaccharidesare also called simple sugars and have the formula (CH2O)n. Monosaccharidescannot be broken down into smaller sugars
Ring form of sugars• There are two forms of
the ring structure (α-glucose and β-glucose), which differ only in the placement of the —H and —OH attached to carbon 1.
• The α and β forms (called anomers) interconvert and exist in equilibrium when dissolved in water.
Glycosidic linkages
Covalently link monosaccharides into larger units
such as disaccharides,
oligosaccharides, and
polysaccharides
Digestible
PolysaccharidesThe polysaccharides are sugar polymers containing more than 20 or so monosaccharide units; some have hundreds or thousands of units. Some polysaccharides, such as cellulose, are linear chains; others, such as glycogen, are branched. For example
Cellulose: Glucose polysaccharide β Linkages Starch: Glucose polysaccharide α LinkagesGlycogen: Glucose polysaccharide Branched
Polysaccharides
Modified Carbohydrates
Lipids Lipids are a class of biological
molecules defined by low solubility in water and high solubility in nonpolar solvents. As molecules that are largely hydrocarbon in
nature, lipids represent highly reduced forms of carbon and, upon oxidation in
metabolism, yield large amounts of energy. Lipids are thus the molecules of choice for
metabolic energy storage.
• Water insoluble due to nonpolar covalent
bonds
• Hydrophobic molecules aggregate together (by hydrophobic and Van der Waals force)
1) Store energy as triglycerides
2) Phospholipids form cell membranes
3) Carotenoids help plants capture light energy
4)Steroids are hormones and vitamins
5) Animal fat is thermal insulator
6) Insulation of nerves
7) Water repellant for skin, fur and feathers
Fats and oils are triglycerides, composed of three fatty acids covalently bonded to a glycerol molecule by ester linkages.
Triglycerides
Lipids: Saturated and unsaturatedSaturated fatty acids have a hydrocarbon chain with
no double bonds.
The hydrocarbon chains of unsaturated fatty acids have one or more double bonds that bend the chain,
preventing close packing.
Phospholipids
Phospholipids contain fatty acids bound to glycerol by ester linkages. In phospholipids, however, any one of several phosphate-containing compounds replaces one of the fatty acids. The phosphate functional group has a negative electric charge, so this portion of the molecule is hydrophilic, attracting polar water molecules. But the two fatty acids are hydrophobic, so they tend to aggregate away from water.
A variety of polar groupsare esterified to the phosphoric acid moiety of the molecule. The phosphate, together with such esterified entities, is referred to as a “head” group
Lipids in Aqueous Cell Environment
The interactions of the hydrophobic tails and hydrophilic heads of phospholipids generate a
phospholipid bilayer that is two molecules thick. The head groups are directed outward, where they
interact with the surrounding water. The tails are packed together in the interior of the bilayer.
Lipids in Vitamins and hormones
Vitamins are small molecules that are not synthesized by the body, but are necessary for its normal functioning. There are four lipid soluble vitamins- vitamin A,D,E and K.
Many hormones are also lipid in nature e.g cortisol.
Nucleic Acids
• Nucleic acids are linear polymers of nucleotides.
• Nucleotides have three characteristic components: (1) a nitrogenous (nitrogen-containing) base, (2) a pentose, and (3) a phosphate.
• Nucleotide without the phosphate group is called a nucleoside.
Nitrogenous bases• The nitrogenous bases are derivatives of
two parent compounds, pyrimidine and purine.
• Both DNA and RNA contain two major purine bases, adenine(A) and guanine(G), and two major pyrimidines.
• In both DNA and RNA one of the pyrimidines is cytosine(C), but the second major pyrimidine is not the same in both: it is thymine(T) in DNA and uracil (U) in RNA
Purines are double ring bases e.g. adenine & guanine Pyrimidines are single ring bases e.g. cytosine, thymine, uracil
Nucleoside • Nucleosides are compounds formed when a
base is linked to a sugar. Nucleosides are composed of : (1) a nitrogenous base, (2) a pentose
• Examples :
Adenosine
Guanosine,
Cytidine,
Thymidine,
Uridine
Nucleotides • Nucleotides have three characteristic
components: (1) a nitrogenous base, (2) a pentose, and (3) a phosphate.
• Examples:
Adenylate (AMP)
Guanylate (GMP)
Cytidylate (CMP)
Thymidylate (TMP)
Uridylate (UMP)
Nucleic acids• Nucleic acids are
linear polymers of nucleotides
• Examples: deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)
structure• In both RNA and
DNA, the backbone of the macromolecule consists of alternating pentose sugars and phosphates (sugar—phosphate—sugar—phosphate). The bases are attached to the sugars and project from the chain.
• The nucleotides
are joined by phosphodiester linkages between the sugar of one nucleotide and the phosphate of the next .The phosphate groups link carbon 3 in one pentose sugar to carbon 5 in the adjacent sugar.
• Most RNA
molecules consist of only one polynucleotide chain. DNA, however, is usually double-stranded; it has two polynucleotide strands held together by hydrogen bonding between their nitrogenous bases.
• The two strands of
DNA run in opposite directions. This antiparallel orientation is necessary for the strands to fit together in three-dimensional space.
• The uniqueness of a nucleic acid resides in its nucleotide sequence
• Only four nitrogenous
bases—and thus only four nucleotides—are found in DNA: adenine (A), cytosine (C), guanine (G), and thymine (T). In double-stranded DNA, adenine and thymine always pair (A-T), and cytosine and guanine always pair (C-G). (complementary base pairing. )
Differences between
DNA• Double stranded• Contains
deoxyribose• Contains thymine• Stores genetic
information
RNA• single stranded• Contains ribose
• Contains uracil• Participates in the
expression of genetic information stored in the DNA
• Three types rRNA, mRNA, tRNA
Nucleic Acids
DNA Double Helix has Uniform Width Information in Sequence not Shape
RNA: Genetic Material and Enzyme
Many viruses use RNA as their hereditary material
RNAs can achieve chemical catalysis, like enzymes e.g. in ribosome the active site is composed entirely
of RNA . Thesecatalytic RNAs are called ribozymes.
QUESTIONS??