SPERMATOGENESIS

PADA IKAN

Def :

Proses pembentukan sperma (spermatogonia)

Meiosis

spermatogenesis

spermiogenesis dan spermiasi

TESTIS TERDIRI :

tubulla longitudinalis (cyste seminiferus

mengandung sel-sel spermatogenik yang

berdiferensiasi sinkroni

dikelilingi sel Sertoli / sel Nutrient).

Testicular Histology

Scanning Electron Micrograph

Light Micrograph

Seminiferous Tubules

Interstial Tissue

SEL SERTOLI (SEL NUTRIENT)

Ciri-ciri : Bentuk pyramidal panjang bertautan Melekat lamina basalis Ujung sel menjorok ke dalam lumen

tubulus / lobulus

Fungsi : Penyokong, pelindung dan mengatur

nutrisi sel-sel spermatogenik Fagositosis (produk berlebih)

resorbsi lisosom sel Sertoli Sekeresi cairan

Sel Leydig (sel Interstitial)

Ciri-ciri :

Muncul setelah dewasa

Bentuk bulat / poligonal

Mempunyai inti di tengah

Sitoplasma eosinofil

Fungsi :

Mensekresi hormone testosterone (meiosis dan maturasi).

Mengatur perkembangan kelamin sekunder (dimorfisme dan dikromatisme).

SPERMATOGENESIS

- Proliferasi

- Tumbuh Spermatogenesis

- Mature

- Trasnformasi = Spermiogenesis

Metamorfosa

Cytological differentiation of spermatogenesis

SPERMATOGONIA MITOSIS

Spermatosit primer (4n)

spermtosit sekunder (2n)

spermatid (n)

spermatozoa

milt oleh vas deferens

air

Spermatogonium Spermatid : Spermatogenesis

Spermatid Spermatozoa : Spermiogenesis

- aparatus golgi Akrosom

- inti kepala spermatozoa

- sentriol ekor spermatozoa

- plasma membran selubung tubuh

- mitokondria berkumpul di ekor

Spermatogonium- dekat lamina basalis, besar bervariasi

- inti bulat lonjong, + butir-butir kromatin

- nukleoli dekat selaput inti

- sitoplasma granuler, < jelas

- HE = inti bulat, biru (kadang-kadang mitosis)

Spermatosit Primer

- Produk akhir spermatogonium B

- inti paling besar, sentral

- interfase = (+) butir-butir kromatin halus

dan rata

Spermatosit Sekunder

- produk meiosis I

- sel tampak bulat ~ spermatogonium

- inti bulat, sentral

- interfase = Inti seperti Spermatosit primer

dengan spermatid muda

Spermatid- produk meiosis II

- hampir berbatasan dengan lumen tubulus seminiferus

- inti eksentris, lonjong

- berkelompok 4 – 8

- daerah golgi dekat inti, berbatas tidak jelas

- mitokondria (butir-butir >>) dalam membran sitoplasma

SpermatozoaTubulus seminiferus berkala dewasa kelamin

- mamalia = 50 – 60

- kepala

leher

ekor = - pangkal

- tengah

- ujung

http://www.fed.cuhk.edu.hk/~johnson/photomicrographs/animal/Reproductive/Testis_TS_LP_2.5x4_H.jpg

LOBULAR LUMEN

Sel Sertoli

Spermatogonium

Spermatocyte

Spermatid

Sperma

Sperm Duct

LOBULAR TUBULAR

Figure 6. Seminiferous tubule, transverse section

1. stem cell

2 & 3 spermatogonium

4. 5. spermatogonium

6. 1st spermatocytes

7. 2nd spermatocytes

8. spermatids

9. mature spermatozoa

10. Leydig

11. blood vessel

FIG. 1. Chinook salmon testes showing stages of germ cell development

Bar =20 µm

primary A spermatogonia transitional spermatogonia late B spermatogonia

primary spermatocytes secondary spermatocytes spermatids (arrow) and spermatozoa (arrowhead)

Spermatogonia

Spermatogonia

Intermediate spermatocytes

Mature spermatazoa

Spermatozoa tails

Spermatozoa heads

SEM of fish sperm

SPERM COMPARISON

NOTE THE 5 MICRON SIZE.

Remember 1000 microns = 1 mm, 25 mm = 1 inch

SPERM MOTILITY The sperm moves by rotating its tail in a spiral motion through

the water. This induces waves of force backward propelling the sperm forward. If the sperm hits a hard surface, like an egg, the spiral motion will cause the entire sperm to rotate.

SPERM MOTILITY The sperm moves by rotating its tail in a spiral motion through the

water. This induces waves of force backward propelling the sperm forward. If the sperm hits a hard surface, like an egg, the spiral motion will cause the entire sperm to rotate.

Sperma bergerak :

Air laut media bersifat hipertonik

Air tawar media bersifat hipotonik

Sperma aktif bergerak dalam air : 30-60’

(hilang setelah 5”).

Sperma ikan teleostei : (-) akrosom

mikrofil (fertilisasi 45 – 60’).