sperm transport in the male tract animal science 434
TRANSCRIPT
Animal Science 434
Lecture 15: Gamete Transport
Sperm Transport in the Male TractFluid Flow Cilia
Fluid FlowMuscle Contractions
Sperm Transport in the Male Tract
• No protein synthetic ability• addition or loss of plasma membrane proteins and lipids• redistribution of proteins and lipids within sperm• changes in lipid diffusion coefficients
Acquisition ofFertilizing Ability
and Motility
Sperm Transport in the Male Tract
Addition ofDecapacitation
Factor Addition of DFFrom AccessoryGland Secretions
Decapacitation Factor• protein, glycolipid and/or lipid• stabilizes the plasma membrane
Site of EjaculationSite of ejaculation Semen Characteristics Species
Vagina slight coagulation of ejaculate human, rabbitvagina semen with high sperm cattle, sheep
concentration
uterus voluminous, distention of cervix horsecervix, uterus voluminous, retention of penis dog, pig
during copulationuterus vagina plug rodents
Sperm Loss From Female Tract
• retrograde flow• phagocytosis by neutrophils
Experiment on Retrograde Flow inthe Cow
Uterine Horn
Uterine Body
Cervix
Inseminationat 3 different
spots
0
5
10
15
20
Cum
ulat
ive
% S
perm
Re
cove
red
From
Vag
ina
0 1 2 3 4 5 6 7 8Hour After Insemination
Uterine Body
Uterine Horn
Cum
ulat
ive
% S
perm
Rec
over
edFr
om th
e Va
gina
0
10
20
30
40
50
60
cervix
0 1 2 3 4 5 6 7 8
Hour After Insemination
Cum
ulat
ive
% S
perm
Rec
over
edFr
om th
e Va
gina
Hours After Insemination
Cervix
Uterine Horn
Evolutionary Strategies for DealingWith Retrograde Flow of Semen
• coagulation of semen or a plug• concentrated semen• semen deposited in uterus
Sperm Loss From Female Tract
• retrograde flow• phagocytosis by neutrophils
Neutrophile Infiltration of Uterus
Phases of Sperm Transport• rapid
»15-30 min• sustained
»6 - 12 hr• oviductal
»at ovulation
Evidence for Sustained Transport
Ligatures atUtero-tubal
Junction
Effect of Oviduct Transection onFertilization in Heifers
Interval from mating* to Oocytes Fertilizedtransection of UTJ
6 0%8 50%
12 83%
* Mating was done at the start of estrus.
4540353025201510500
20
40
60
80
100
Effect of Isthmus Transection on Fertilization in the Pig
Time From Mating to Transection (hr)
Pe
rce
nta
ge
of
Oo
cy
tes
Fe
rtil
ize
d
Control (no transection)
Transected Isthmus Ovulation
Isthmus
Uterus
Ligationto Transectthe Isthmus
Ampulla
Ovary
Sperm Transport
}
2 cm
Sperm Transport in the PigPe
rcen
tage
of O
ocyt
es F
ertil
ized
}
Ovulation
OocyteCumulus CellsFollicular Fluid Cue for ovulation may
effect isthmus to triggeroviductal transport
6-12 hr18-24 hr 1-2 hrLocation of Fertilizing Sperm Barriers To Sperm Transport
Cervix
Barriers To Sperm Transport
Uterotubal Junction
Ovulation
OocyteCumulus CellsFollicular Fluid
Fluid Flow
Factors Regulating OocyteTransport in the Oviduct
Remains closed untilday 3 - 5 post ovulation
Toward Uterus
TowardAmpulla
Total Fluid Production
Factors Regulating OocyteTransport in the Oviduct
Cilia
Fluid FlowFluid FlowMuscleContractions
No Contractions
Remains closed untilday 3 - 5 post ovulation
Site ofFertilization
Site ofFertilization
Now view the videopodcast entitled
Ovulation and Oocyte Transport
Obtain from Lean@UW podcastlinks for this course in theLecture area.