Immunhistology

Solutions with system

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ZYTOMED SYSTEMS GmbH Anhaltinerstraße 16 14163 Berlin Fon +49 30 804 984 990 Fax +49 30 804 984 999info@zytomed-systems.com www. zytomed-systems.com

Somatostatin Receptor antibodies

Somatostatin Receptor SSTR2A and SSTR5 antibodies – New prognostic toolsZytomed Systems now offers two new, highly specific and affinity-purified antibodies for de-tection of the somatostatin receptor subtypes SSTR2A and SSTR5. Both antibodies are especially developed for immunohistochemistry on FFPE tis-sue sections.SSTR2A and SSTR5 are very promising new mar-kers. For some tumours, especially neuroendocri-ne tumours, somatostatin analogues are used for therapy. The targets of these therapeutically active agents are somatostatin receptors on the tumour cell surface. It can be very helpful for the physician to know if the receptors are expressed on the cell.Somatostatin receptors moderate the effect of somatostatin in many tissues via different path-ways. For example, somatostatin inhibits the growth hormone secretion in the pituitary gland. The pituitary gland and the spleen express high levels of all receptor types. The subtypes 2A and 5 are the most important receptors in humans because all somatostatin analogues bind to these subtypes.

SSTR2A and SSTR5 are highly expressed in the cyto-plasmic membrane in a variety of human tumours. The expression in neuroendocrine tumours (NET) of the pancreas, lung and gastrointestinal tract is of spe-cial interest because depending on the tumour type 40 – 80% of these are positive for SSTR2A and SSTR5.“The analysis of the SSTR status specifically for each patient, and studies of individual tumour biological behaviour, might be of therapeutic in-terest and could help to optimise treatment espe- cially in unresectable tumours.” says M. Appetec-chia from the Regina Elena National Cancer Insti-tute in Rome. Determining the expression level of SSTR2A and SSTR5 may help to decide which pati-ents are suitable for treatment with somatostatin analogues (Octreotide, Lanreotide).Our new antibodies against the somatostatin re-ceptor subtypes SSTR2A and SSTR5 are produced in cooperation with Prof. Stefan Schulz MD, one of the leading experts on this topic. Both antibodies are affinity-purified and therefore allows for a very clear membranous staining.

SSTR2A on pancreas (AP – Permanent AP Red)

SSTR2A on carcinoid (HRP – AEC)

SSTR5 on pancreas (HRP – DAB)

Description Reactivity Method Pre-treatment Dilution Volume Cat. No

Somatostatin Receptor 2A SSTR2AClone: polyclonalHost: Rabbit

HU, MS,RT

P EDTA pH 9.0 1:100 0.5 ml RBK046-05

Somatostatin Receptor 5 SSTR5Clone: polyclonalHost: Rabbit

HU P Citrate pH 6.0 1:100 0.5 ml RBK051-05

Product information

Bibliography

[1] Plöckinger U, Albrecht S, Mawrin C, Saeger W, Buchfelder M, Petersen S, Schulz S „Selective loss of somatostatin receptor 2 in Octreotide-resistant growth hormone secreting adenomas“ J Clin Endocrinol Metab 93:1203-1210, 2008

[2] Fischer T, Doll D, Jacobs S, Kolodziej A, Stumm R, Schulz S „Reassessment of sst2 somatosta-tin receptor expression in human normal and neoplastic tissues using the novel rabbit mono-clonal antibody UMB-1“ J Clin Endocrinol Metab 93:4519-4524, 2008

[3] Asnacios A, Courbon F, Rochaix P, Bauvin E, Cances-Lauwers V, Susini C, Schulz S, Boneu A, Guimbaud R, Buscail L “Indium-111-pente-treotide scintigraphy and somatostatin receptor subtype 2 expression: New prognostic factor for malignant well-differentiated endocrine tumors.” J Clin Oncol 26:963-970, 2008

[4] Appetecchia M, Baldelli R “Somatostatin ana-logues in the treatment of gastroenteropancrea-tic neuroendocrine tumours, current aspects and new perspectives” J Exp Clin Cancer Res 29:19, 2010

[5] Heaney AP, Melmed S “Molecular targets in pituitary tumours” Nat Rec Cancer 4:285-295, 2004

ER on breast stained with AP-Poly-mer Kit and Permanent AP-Red

MSH-2 on colon stained with HRP One-Step Polymer and DAB

S-100 on nerve tissue stained with AP-Polymer Kit and Permanent AP-Red

E-Cadherin on breast stained with HRP One-Step Polymer and AEC

Immunhistology

Solutions with system

Description Volume Cat. No.

ZytoChem Plus (HRP) Polymer Kit1 Kit (6 ml / 60 tests) POLHRP-006

1 Kit (100 ml / 1000 tests) POLHRP-100

ZytoChem Plus (HRP) One-Step Polymer anti-Mouse/Rabbit/Rat

6 ml / 60 tests ZUC053-060

100 ml / 1000 tests ZUC053-100

ZytoChem Plus (HRP) Polymer anti-Rabbit6 ml / 60 tests ZUC032-060

100 ml / 1000 tests ZUC032-100

ZytoChem Plus (HRP) Polymer anti-Mouse6 ml / 60 tests ZUC050-060

100 ml / 1000 tests ZUC050-100

ZytoChem Plus (AP) Polymer Kit1 Kit (6 ml / 60 tests) POLAP-006

1 Kit (100 ml / 1000 tests) POLAP-100

All mentioned Zytochem Plus Polymer kits are suitable for the detection of primary antibodies obtained from mouse and rabbit. They contain ready-to-use reagents of Blocking Solution, Post Block, and Enzyme-Polymer. The species reactivity of the single reagents is mentioned respectively.

Description Volume Cat. No.

AEC Single Solution (Ready-to-use)8 ml ZUC037-008

100 ml ZUC037-100

AEC Substrate Kit(Chromogen concentrate and substrate buffer)

1 Kit (500 tests) ZUC042-050

1 Kit (5000 tests) ZUC042-500

Permanent AEC Kit (4 components; alcohol-resistant, for permanent mounting)

1 Kit (2000 tests) ZUC054-200

DAB Substrate Kit (Chromogen concentrate and substrate buffer)

50 ml / 500 tests DAB057

500 ml / 5000 tests DAB530

Permanent AP-Red Kit (2 components; alcohol-resistant, for permanent mounting)

125 ml / 1250 tests ZUC001-125

550 ml / 5000 tests ZUC001-500

Fast-Red Substrate Pack (Tablets and Buffer)

55 ml / 500 tests RED055

125 ml / 1250 tests RED125

550 ml / 5000 tests RED500

Description Volume Cat. No.

Antibody Diluent100 ml ZUC025-100

500 ml ZUC025-500

HIER* Citrate Buffer pH 6.0 (10X)100 ml (for 1000 ml) ZUC028-100

500 ml (for 5000 ml) ZUC028-500

Pink PAP Pen 1 pen ZUC064

Pink PAP Pen Mini 1 pen ZUC065

Wash Buffer (20X)500 ml (for 10 litre) ZUC020-500

2500 ml (for 50 litre) ZUC020-2500

* HIER = Heat Induced Epitope Retrieval.

High sensitivity detection systems polymer technique

Chromogens

Accessories

Detection systems and accessory reagents

A062/2011/E