snc2d lesson 2 microscopes - science to a tee...biologists use different types of microscopes to...
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MICROSCOPES
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1.2.Dunia
Sage
ELECTRON MICROSCOPE
ELECTRON MICROSCOPE
ELECTRON MICROSCOPE
★The ELECTRON microscope uses a beam of electrons that penetrate or reflect of the specimen to produce a highly magnified sharper image
★magnification can reach 1-5 million time the human eye.
★Transmission E.M.- produces flat images
★Scanning E.M.- produces 3D looking images
ELECTRON MICROSCOPE
ELECTRON MICROSCOPE
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THE LIGHT MICROSCOPE AS A TOOL FOR CELL RESEARCH○ Biologists use different types of microscopes to
explore cell structure and function.
○ Cells can be viewed under a microscope to look for abnormalities.
THE LIGHT MICROSCOPE
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COMPOUND LIGHT MICROSCOPE
★The compound light microscope uses visible light that is projected through sets of lenses. •the objective and eye piece
★Typically ranges from 40x to 2,000x the human eye.
Objective lens= 40x eyepiece (ocular) is =10x Total magnification 40 x 10 = 400x
COMPOUND LIGHT MICROSCOPEPart Function
1. Revolving nosepiece
Holds the objective lenses
2. Objective lenses
Magnify specimen; three lenses are usually 4X, 10X, and 40X
3. Stage Supports the slide for observation
COMPOUND LIGHT MICROSCOPEPart Function
4. Diaphragm and Iris
pass light and adjusts the light through the specimen
5. Condenser lens
Focuses light onto the specimen
6. Fine adjustment knob
Sharpens an image
COMPOUND LIGHT MICROSCOPEPart Function
7. Coarse adjustment knob
Moves the stage up or down to focus on the specimen
8. Stage clips Hold the slide in position on the stage
9. Eyepiece Magnifies the specimen, usually by 10X; single lens
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RESOLUTION○ Being able to see clear detail in an image depends
on the resolution ○ Resolution is the ability to distinguish between
two objects that are very close together.
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1.2.Sage
Find and focus on the specimen
○ The LOW RULES
1. low stage 2. lowest magnification 3. low light
Preparing a Wet Mount
A. Place a drop of water on a coverslip
B. Float specimen on the water drop
C. Place a coverslip over the slide (folding it over carefully as to not trap air bubbles
D. Apply a drop of stain to the edge of the cover slip
○
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Reviewing Cell drawings
1. Use a sharp pencil
2. Draw a field of view
3. Draw cells with a clean contour line (stippling may be used)
4. Label using a ruler
5. Only a few cells required (3-5 with tissue)
6. Estimate the size of the specimen with the light microscope.
7. Title and magnification of the image used.
Measuring SizeMagnification 10 x 10 = 100x
1. Go to 100x magnification- (10 eye piece x 10 objective) 2. Use the scale of diameter = 1mm or 1000 um 3. Estimate size
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LAB
Biological drawings
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Biological drawings
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Biological drawings