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8/3/2019 Science Presentation-Analgesic Effect New Synthetic Drugs

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1/25/2012 1

ANALGESIC EFFECT OF NEW

SYNTHETIC DRUG BENZOFURAN-3,4-DIHYDROXY CHALCONE BY

HOT PLATE TESTS IN MICE

Theresia Ayu. Rayon 2443009070Edwin Jeika B. 2443009108


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OUTLINE

Introduction

Materials Methods

Results & Disscusion

Conclusions

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INTRODUCTION

Analgesic drugs relief pain

??

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Effiicacy &Unwanted

Effect

Need new

better drugs


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INTRODUCTION

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ChalconeDerivates

Anti-inflammatory effect andcancer chemo preventiveactivity

Hydroxy chalcones inhibit 12-lipoxygenase & cycloxygenase

Benzofuran-3-one Derivates

Anti-inflammatory effectRapidly and extensivelymetabolized after systemicadministration nontoxic


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INTRODUCTION : Purpose

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ChalconeDerivates

DerivatBenzofuran-3-one

Activity Tests


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MATERIALs

Synthesis

Pharmacology

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5-MethoxybenzofuranOxalyl ChlorideDiazomethane

Acetic Acid3,4-DihydroxybenzaldehydeDry HCl

Mice: Male SwissAlbino

with good housing


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METHODs

Synthesis ofDrugs:

Reaction &Crystalilzed

Pharmacology:

Analgesic Effecttested by Hotplate method

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O

O

O

Cl

2,5-dimethoxybenzoyl chloride

N+

N-

diazomethan

O

O

O

N N

2-diazo-1-(2,5-dimethoxyphenyl)ethanone

O

O

O

OH

2,5-dimethoxybenzoic acid

O

O

Cl

Cl

oxalyl dichloride

O

O

O

Cl

2,5-dimethoxybenzoyl chloride

Step 1

Step 2

Method: Synthesis

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O

O

O

N N

2-diazo-1-(2,5-dimethoxyphenyl)ethanone

O

OH

acetic acid

O

O

O

5-methoxybenzofuran-3(2H)-one

O

O

O

5-methoxybenzof uran-3(2H)-one

O

OH

OH

3,4-dihydroxybenzaldehyde O

O

OOH

OH

(z)-2-(3,4-dihydroxybenzylidene)-5-methoxybenzofuran-3(2h)-one

Step 3

Step 4

Method: Synthesis

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Benzofuran-3,4-dihydroxy

chalcone (BF-DK)


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Method : Hot Plate

Temperature 551C

Observation

0, 15, 30, 45, and 60 min after i.p.

lick the fore or hind paw or jump out

Quantitatif Maximum Possible Effect

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Method : Hot Plate

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Group 1 2 3 -controll +controll

Drug BF-

DK

BF-

DK

BF-

DK

Saline Morphin

e

Ibuprofen

Doses(mg/kg)

12,5 25 50 10ml/kg 2,5 200


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RESULT & DISCUSSION

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RESULT & DISSCUSION

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RESULT & DISSCUSION

Concentration of BF-DK, reached to max in

45 minutes after injection Metabolized

In acute phase, BF-DK equal to ibuprofen Analgesic effect of Benzofuran-3,4-dihydroxy

chalcone is lower than Morphine (central

analgesic)

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CONCLUSION

Drug have analgesic effect

Further research can be developing to

investigation the analgesic mechanisms ofthe drug

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science presentation-analgesic effect new synthetic drugs

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