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How do microscopes work?

Light Microscopes◦ Produce magnified images by focusing visible

light.◦ Staining can be used to show structures in the

cell.◦ Can magnify up to 1000x

Electron Microscopes◦ Produce magnified images by focusing a beam of

electrons◦ Some can magnify 100,000x

Eyepiece – contains magnifying lens Arm – supports the body tube Stage – supports the slide being observed Body Tube – Maintains the proper distance between the

eyepiece and the objectives Focus- Fine and course adjustments move the body

tube to focus the image Objective – Provides magnification. Total magnification

= eyepiece times objective Condenser or diaphragm – controls the amount of light

passing up to the eyepiece Mirror or illuminator – produces or reflects light up

toward the eyepiece

How image is created

Advantage Disadvantage

Light Microscope

Scanning Electron Microscope

Transmission Electron Microscope