RNAi

Mitchell Planty

Quick Overview

RNAi– Natural regulatory mechanism for gene expression.

– Protection mechanism against overproduction of harmful

products

– Transmittable to other cells.

– Transmittable to offspring

dsRNA cell dsRNA + Dicer enzyme siRNA

siRNA + RISC mRNA = X

Useful?

Gene Identification

Gene Therapy

Delivery?

MSNs

M-MSNs

No negative effects

Example of Application

Aurora-A kinase

Laryngeal caner cell line Hep-2

Blocked by RNAi using a plasmid delivery

Suppressed Aurora-A production by 52%

Tumor size from Hep-2 line 7-10 cm

Tumor size from interfered line 1-3 cm

Works Cited

Campbell, Mary K., and Shawn O. Farrell. "13.8." Biochemistry. Belmont, CA: Brooks/Cole Cengage Learning, 2012. 385. Print.

"RCSB PDB - 1MQ4 Structure Summary." Web. 30 Mar. 2012. http://www.rcsb.org/pdb/explore/explore.do? structureId=1MQ4.

X, Li, Xie QR, Zhang J, and Gu H. "The Packaging of SiRNA within the Mesoporous Structure of Silica Nanoparticles." National Center for Biotechnology Information. U.S. National Library of

Medicine. Web. 30 Mar. 2012. http://www.ncbi.nlm.nih.gov/pubmed/21906804.

Zang, H., XH Chen, CP Cai, SL Wang, BY Liu, and L. Zhou. "[Suppression of Aurora-A by RNA Interference Inhibits Laryngeal Cancer Hep-2 Cell Growth]." National Center for Biotechnology Information. U.S. National Library of Medicine. Web. 30 Mar. 2012. <http://www.ncbi.nlm.nih.gov/pubmed/ 22455777>.