GREEN SYNTHESIS OF SILVER

NANOPARTICLES USING

PLANT EXTRACT

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By

Abhishek Indurkar

Assignment for Research Methodology

IntroductionSilver Nanoparticles

• Nanotechnology is manipulation of matter on an atomic and molecular range.

• Size of nanoparticles = between 1 and 100 nanometer (1 nm = 10-9 m) in size.

• Silver nanoparticles show wide application in the field of nanomedicine.[1]

• Applications of nanotechnology

1. Nanomedicine

2. Nanoelectronics

3. Biomaterials energy production.[2]

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KIT,s College of Engineering, Kolhapur

Green Synthesis of Silver Nanoparticles

• Silver nanoparticles can be synthesized by several methods.

• They can be divided into three broad categories:

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Synthesis of Silver Nanoparticles[2]

1. Physical synthesis

2. Chemical synthesis

• Using plant extract[2]

• Using antibiotics[2]

• Using microorganisms[2]

3.Biogenic synthesis

• The biological synthesis of nanoparticles has provided a means for improved

techniques compared to the traditional methods that call for the use of harmful

reducing agents.

• Chemical and Physical methods for the synthesis are very costly, toxic and affects

the medical applications.

• Biogenic methods of silver nanoparticles synthesis are ecofriendly and produce

nanoparticle with precise shape and size.[2]

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5

[Ref 3]

Main topic

Green Synthesis of Silver Nanoparticles

Using Plant Extract

• Biosynthesis of nanoparticles is considered a green technology, as it does not involve

any harmful chemicals.

• Several microorganisms, such as bacteria, fungi and yeasts have come up as Nano-

factories, synthesizing metal nanoparticles of Ag and Au.

• However, the use of plants for the fabrication of nanoparticles has drawn attention,

because of its rapid, economical, eco-friendly protocol, and it provides a single step

technique for the biosynthesis process.

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Notes

• The synthesis of silver nanoparticles depend on phenolic content of different plant sources.

Ag+ reduction Ag0

• Different parameters like temperature, time, pH, etc. influence the size and shape of silver

nanoparticles.

• Antimicrobial activity of AgNPs can be studied using Well diffusion assay.

• Applications :

1. Effluent treatment process (for reducing the microbial load)[7]

2. Wound dressing

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Methodology

1. Preparation of plant extract

2. Synthesis of silver nanoparticles

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Ag+ ions were reduced by addition of plant extract to aqueous AgNO3 solution.

Reduction of the Ag+ ions was monitored by measuring the ultraviolet-visible spectrum of the solution at regular intervals on a spectrophotometer operating at a resolution of 1

nm.

The effects of temperature on the rate of synthesis and size of the prepared silver nanoparticles were studied by carrying out the reaction in a water bath at particular

temperature with reflux.

The concentration of AgNO3 solution was also varied

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Analysis of silver

nanoparticles

Scanning Electron

Microscope

UV Visible

Spectrophotometer

Transmission

Electron Microscop

e

X-ray Diffraction

Fourier Transform Infrared

Spectroscopy

Assignment for Research Methodology

Categorisation

The parameters like reaction time and size of nanoparticles are considered.

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Sr. No. Source Time Of Reaction Size (nm) References

1. Garcinia morella 25 min 120.1 Borah D., et.al[5]

2. Citrus sinensis 1 hr 50-73 D.Subhashini [6]

3. Pomelo Fruit 24 hrs 2.5-5.7 D. Sarvamangala[8]

4. Tribulus terrestris 36 hrs 24.63 V. Gopinath[9]

5. Aloe vera 24 hrs 500 Hashoosh S.I,et.al[3]

6. Lemon Extract 24 hrs 30.5 Selvakumar P,et.al[10]

7. Banana peel extract 72 hrs 23.7 Ibrahim H.M.M [7]

8. Oak fruit bark 30 min 20-25 Veisi H.,et.al [11]

9. Carambola fruit extract 1 hr 18 Mane Gavade S J. [12]

10. Apple Extract 1 hr 30.25 Abidin Ali Z.,et.al [13]

11. Malus domestica 24 hrs 20 Roy k.,Et.Al [14]

Detailed Outline

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Synthesis of silver nanoparticles

Chemical Physical Biological

Micro-organisms Antibiotics Plant extract

Parameters

Time Size

References

Slide # 5,6, 7.

Literature Slide # 8,9,10

Narrowing topic

Slide # 2,3

Introduction

Slide # 1

Detailed Outline

• Introduction

• Notes

• Out of different methods, biogenic methods of synthesis of silver nanoparticles were selected.

• Green synthesis of silver nanoparticles using plant extract (mainly fruit extract) was selected.

• Different parameters in the synthesis of silver antibiotic were studied. Reaction time and size of nanoparticles are selected for detailed study.

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THANK YOU…

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References

1. Satoshi H. and Serpone N., (2013).“Microwave in nanoparticle synthesis”, first edition, Wiley-VCH

Verlag GmbH & Co. KGaA.

2. Rauwel P. (2015) “silver nanoparticles: synthesis, Properties and applications”, Hindawi Publishing

cotrpotratrion, Advances in material Science and engineering.

3. Priyanka Singh., (2016), “Biological Synthesis of Nanoparticles from Plants and Microorganisms”,

vol 34, p588–599.

4. Ibrahim H.M.M., (2015)“Green synthesis and characterization of silver nanoparticles using banana

peel extract and their antimicrobial activity against representative microorganisms”, Journal of

Radiation Research and Applied Sciences, p265-275. [IMF above 2]

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Bibliography

1. Satoshi H. and Serpone N., (2013).“Microwave in nanoparticle synthesis”, first edition, Wiley-VCH Verlag GmbH & Co. KGaA.

2. Rauwel P. et al., (2015) “silver nanoparticles: synthesis, Properties and applications”, HindawiPublishing cotrpotratrion, Advances in material Science and engineering.

3. Singh P. et al., (2016), “Biological Synthesis of Nanoparticles from Plants andMicroorganisms”, vol 34, p588–599.

4. Hashoosh S. I.,et.al, (2014)“Production of Ag nanoparticles Using Aloe vera Extract and itsAntimicrobial Activity”, Journal of Al-Nahrain University, Vol.17 (2), p165-171.

5. Borah D. & Yadav A., (2015) “A Novel ‘Green’ Synthesis of Antimicrobial SilverNanoparticles (AgNPs) by using Garcinia morella (Gaertn) Desr. Fruit Extract” Nanoscience& Nanotechnology-Asia,5, p25-31.

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6. Subhashini D., (2015) “Antibacterial potency of nanosilver synthesized from the fruitfiltrate of citrus sinensis”, International Journal of Pharma and Bio Sciences, p396 – 403. [IMF 5.12]

7. Ibrahim H.M.M., (2015)“Green synthesis and characterization of silver nanoparticles usingbanana peel extract and their antimicrobial activity against representative microorganisms”,Journal of Radiation Research and Applied Sciences, p265-275. [IMF 2.18]

8. D. Sarvamangala, (2013)“Biogenic Synthesis of AGNP’s Using Pomelo Fruit –Characterization and Antimicrobial Activity against Gram +Ve and Gram –Ve Bacteria”,International Journal of Pharmaceutical Sciences Review and Research, p30-35. [IMF 2.54]

9. Gopinath V. et al., (2012) “Biosynthesis of silver nanoparticles from Tribulus terrestris andits antimicrobial activity: A novel biological approach”, Colloids and Surfaces B:Biointerfaces, 69– 74 [IMF 2.18]

10.Selvakumar P. et al., (2016)“Green Synthesis and Antimicrobial Activity ofMonodispersed Silver Nanoparticles Synthesized Using Lemon Extract”, Synthesis andReactivity in Inorganic, Metal-Organic, and Nano-Metal Chemistry, 291–294

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11. Veisi H. et al., (2016) “Green synthesis and characterization of monodispersed silver

nanoparticles obtained using oak fruit bark extract and their antibacterial activity”,AppliedOrganometallic Chemistry, vol 30, 387–391[IMF 2.248]

12. Mane Gavade S J., (2015) “Green synthesis of silver nanoparticles by using carambola fruit

extract and their antibacterial activity”, Advances in Natural Sciences: Nanoscience andNanotechnology, vol 6.

13.Zainal Abidin Ali, Rosiyah Yahya, Shamala Devi Sekaran, and R. Puteh, “Green Synthesis of

Silver Nanoparticles Using Apple Extract and Its Antibacterial Properties,” Advances inMaterials Science and Engineering, vol. 2016, Article ID 4102196, 6 pages, 2016.doi:10.1155/2016/4102196.

14. Roy K., Sarkar C., & Ghosh C. (2014) “Green synthesis of silver nanoparticles using fruit

extract of malus domestica and study of its antimicrobial activity”, Vol. 9, p1137 – 11

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