repressilator: “i’ll be back.”

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pressilator: “I’ll be back http://www.freefoto.com/images/11/11/11_11_12---Portland-Bill-Lighthouse_web.jpg

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Repressilator: “I’ll be back.”. http://www.freefoto.com/images/11/11/11_11_12---Portland-Bill-Lighthouse_web.jpg. Repressilator: “I’ll be back.”. Is it possible to get a culture of E. coli cells to flash on and off with a green light?. - PowerPoint PPT Presentation

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Page 1: Repressilator: “I’ll be back.”

Repressilator: “I’ll be back.”

http://www.freefoto.com/images/11/11/11_11_12---Portland-Bill-Lighthouse_web.jpg

Page 2: Repressilator: “I’ll be back.”

Repressilator: “I’ll be back.”

Is it possible to get a culture of E. coli cells to flash on and off with a green light?

A Synthetic Oscillatory Network of Transcriptional Regulators; Michael B. Elowitz and Stanislas Leibler; Nature. 2000 Jan 20;403(6767):335-8.

Page 3: Repressilator: “I’ll be back.”
Page 4: Repressilator: “I’ll be back.”
Page 5: Repressilator: “I’ll be back.”

Repressilator: “I’ll be back.”

Is it possible to get a culture of E. coli cells to flash on and off with a green light?

YES!!

Page 6: Repressilator: “I’ll be back.”

Clonal cells = clonal action?

Page 7: Repressilator: “I’ll be back.”

Repressilator: “I’ll be back.”

Is it possible to coordinate a culture of E. coli cells to flash on and off with a green light?

NO!!

Page 8: Repressilator: “I’ll be back.”

Repressilator: “I’ll be back.”

What worked and what did not?

How could you regulate the repressilator to be synchronous?

Page 9: Repressilator: “I’ll be back.”

Alternative Representations

What if you don’t understand the molecular biology short hand, canthis information be presented in a different way to reach a wider audience?

Page 10: Repressilator: “I’ll be back.”
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Page 12: Repressilator: “I’ll be back.”