Bioreactor & FermentationTechnology at acib

Rent a Bioreactor

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Microbial Fermentation 05

Animal Cell Fermentation 05

Cell Harvest 07

Filtration 08

Separation 08

Analytics 10

Our Services 11

About acib & Contact 12

Content

Rent a BioreactorThe Austrian Centre of Industrial Biotechnology (acib) has been supporting companies and scientific partners for decades. We under-stand how important the right know-how and facilities can be to success-fully conclude projects. We also know, how expensive it can be to install and maintain the latest state-of-the-art equipment, especially in case of huge bioreactors.

The new solution at acibMaking available the expertise and facilities you need for your fermenta-tion and/or bioprocess development. Our experts provide you with offers tailored for your specific project and you choose whether to:

k rent-a-bioreactor / DSP for your projectk train your staffk let us do all the work for you

A detailed list of available equipment for your projects is on the next pages.

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Upstream ProcessingM I C R O B I A L F e R M e n T A T I O n

Fermentation for bacteria, yeast and fungi

k Fully automated process control (Allen Bradley/Rockwell)k Supply with steam, ice water, cooling water and compressed airk 2 modular bioreactors (10 – 20 L, 60 – 100 L)k 30 L and 200 L (working volume) stirred tank reactork Storage/fermentation vessels (1250 L, 2000 L, 3600 L) with double jacket, stirrer and aerationk Additional mobile vessels for storage and media preparation available

Labscale fermentation for bacteria, yeasts and fungi

k Incubation in shake flasksk DASGIP® parallel bioreactor system ( 4 × 1 L bioreactors)k Infors Multifors® II (4 × 2 L bioreactors) with offgas analysis (BlueSense)k Sartorius Biostat® Bplus (2 L bioreactor)k Biostat Ed. (2 × 5 L bioreactors), modified and adaptablek Additional access to high throughput micro bioreactor systems (BioLector®), lab scale bioreactors equipped with online monitoring systems

A n I M A L C e L L F e R M e n T A T I O n

Fermentation of CHO, hybridoma and insect cellsk including automated process controlk 70 L scale stirred tank bioreactor (fully automated)k 500 L airlift bioreactork 1600 L scale stirred tank bioreactork Additional access to small and lab scale bioreactors/systems (shake flask, DASGIP®, WAVE …)

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Downstream ProcessingC E L L H A R V E S T

Centrifugationk Several labscale centrifugesk High performance centrifuge with continuous feed and discountinuous discharge (Contifuge) with 22,000 g and solid volume 0.3 Lk Disc-stack centrifuge (GEA PSC-6) with 8000 m2 equivalent clarification area; 30 – 300 L/h throughput and other Westfalia Disc Separator units

High Pressure homogenization (HPH)k Two-stage high pressure homogenizer – 1500 bark Benchtop system for lab scale and optimization (10 L/h throughput)k Pilot scale system variable (50 – 100 L/h throughput)

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F I L T R A T I O n

Dead-end FiltrationkFilter test system for filter disks (14 cm2) and capsules filtration operation up to 100 L/batch with pressurized closed ves-sels

Ultra-/DiafiltrationkSmall scale (50 – 300 cm2) andkPilot scale systems (up to 2 m2 membrane area) with automated recording of all system data (pressure, fluxes, etc.) kSystems can be operated in different control modes (constant flux, constant trans-membrane pressure, min and max inlet pressure)kSterile filtration for intermediates and final products

Micro- and ultrafiltration (crossflow ceramic modules)k2 microfiltration modules Atech (0.2 µm) with 0.13 m² filter area eachk2 microfiltration modules Atech (0.2 µm) with 0.2 m² filter area eachk2 microfiltration modules Atech (0.2 µm) with 0.58 m² filter area eachk2 ultrafiltration modules Atech (0.05 µm) with 0.2 m² filter area eachkPumps, casings, storage vessels

Special filtration techniqueskVacuum drum filter VELO FRP 1 (1 m² filter area, 500 L/h)

S e P A R A T I O n

Precipitation and refoldingk(Fed-)Batch operation with temperature control, headspace gas overlay and on-line monitoring probes. Operating volume 20 – 100 L (suitable for precipitation and refolding reactions)

ChromatographykÄKTA explorer 10 and 100 for development and small scale preparative purificationkÄKTA Pilot for pilot scale chromatography up to 40 L/h, Column size 0,5 – 10 LkÄKTA purifier and ÄKTA pure (labscale chromatography)

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S e P A R A T I O n

LyophilizationkLabonco Freezone 2.5 plus, labscalekChrist Gamma 1-16 LSC plus, lab-/pilot scale

Fluid bed drying/granulationkAMMAG A-WT 43 multifunctional fluid bed drier for drying, granulation and coating, pilot scale (ca. 50 L batch size)

Distillation/Rectificationkplants of different sizes (DN 10 – DN 150)kdifferent operation modes (discontinuous, continuous)kadjustable for different separation problems at ambient pressure and vacuum operationkthin film evaporatorkrotary evaporators (up to 20 litres)ksteam distillation

Liquid – Liquid Extraction plantskmixer-settler plants of different sizes (ml up to several litres)kextraction columns with different internals (packed, sieve tray, RDC, TCDC and operation mode (agitated, pulsed)

CO2-technologykextraction plant (300 ml, 690 bar, 240 °C up to 10 litres, 325 bar, 200 °C, including stirrer)kimpregnation plantkhigh pressure view cell (400 bar, 100 °C)kmicro-mixer fractionation plant for liquid feed materialkextraction column (2 m active height, 35,4 mm inner diameter, 180 bar, 100 °C, settler with view glasses at bottom and top)

Dryingkspray dryer (MINOR plant, d = 800 mm, h = 600 mm, 80 kg/h air, max. inlet temperature = 340 °C)kfluidized bed dryer

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Membrane unit OSMOkflat sheet membranes for MF, NF, UF, RO (max. 64 bar)

Single separation equipmentkdisc centrifuge (pilot plant size)

A n A LY T I C S

kAcid/base titration, Karl FischerkGC-FID, GC-MS, GC-ECDkHPLCkIon chromatographykAASkTOCkDrying balancekPAAR densiometerkPAAR Stabinger viscosimeterksurface tension (ring, droplet)

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Our ServicesWe provide tailored calculations for each project! In case of sensitive data, we can work under protection of a CDA/nDA. Aside from contract research, we are also looking forward to supporting you in national or international funding projects.

e X A M P L e S

k Process development for up- and downstream processing for bacteria, yeasts, fungi and higher eukaryotic systems

k Fermentation scale up from µ- to lab- and pilot scale

k On-line monitoring platform and development of monitoring strategies for critical points in real-time in up- and downstream

k Manufacturing and processing of products at pilot scale (non-GMP, but “GMP-like” conditions available)

k Available in-house model systems e.g.: rhSOD, GFP, rh-antiTNFα, rhTNFα mAB, scFv (IB or soluble) …

k Available model organisms: E. coli, P. pastoris, CHO, Sf9-insect cells, etc. also open for model systems and organisms provided by partner

k Method development for cell harvest, IB harvest, refolding, precipitation, small scale development and transfer to pilot scale

k Filter testing for depth- and sterile filtration including upscale

k Ultra-/Diafiltration incl. method development and upscale; membrane testing

k Chromatographic separation; method development and upscale; bench marking of different resins with in-house model systems (different feed)

k Test of equipment provided by supplier

k Individual training for single or multi-unit operations including academic level education in bioprocess engineering

k complete process development from determination of thermodynamic data, lab scale experiments and to pilot scale experiments

k experimental validation of the downstream process

k modelling, simulation and feasibility studies of single separation units up to complete processes

k economic and environmental process evaluation

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INNOVATIONS FROM NATURE

F O L L O W U S O N

www.facebook.com/acibgmbhwww.linkedin.com/company/acib-gmbhwww.youtube.com/acib

INNOVATIONS FROM NATURE

The Austrian Centre of Industrial Biotechnology A B O U T A C I B

The Austrian Centre of Industrial Biotechnology (acib) is a top-class international research institution in the fi eld of industrial biotechnology. Since its foundation in 2010 the centre of has specialized on the development of innovative, eco-friendly and economical processes for the biotech-, chemical- and pharmaceutical in-dustries using the methods and tools of nature. Presently 200+ coworkers are carrying out research in more than 175 research projects.

C O n T A C T

acib GmbHDr. Martin TrinkerDirector Business Development& FundraisingPetersgasse 14A-8010 Graz

martin.trinker@acib.at +43 316 873 9316 www.acib.at

INNOVATIONS FROM NATURE

www.acib.at