regulace genov É exprese
DESCRIPTION
REGULACE GENOV É EXPRESE. C E N T R A L D O G M A. I N M O L B I O L O G Y. Initiation is the rate-limiting step for translation main target of the translational regulation. DNA transcription RNA translation - initiation - elongation - termination PROTEIN. P-site. - PowerPoint PPT PresentationTRANSCRIPT
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REGULACE
GENOVÉ
EXPRESE
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CENTRAL
DOGMA
IN
MOL
BIOLOGY
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Initiation is the rate-limiting step for translation
main target of the translational regulation
DNA
transcription
RNAtranslation -
initiation -
elongation -
termination
PROTEIN
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5’ end determination / (f)Met-tRNAiMet recruitment /
mRNA recruitment / the AUG start codon recognitionBACTERIA
Shine-Dalgarno seq. (AGGAGG motif) in every mRNA complementary to 3’ end of 16S rRNA (small subunit)3 so called initiation factors (IFs) => fMet-tRNAi
Met recruitment
EUKARYOTS 5’ 7Methyl-Guanosine Cap 3’ Poly-tail 12 eukaryotic initiation factors (eIFs) Scanning for AUG
P-site
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GeneralTranslation Initiationpathwayand itsregulation
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Main Object of our Studies => roles of eIFs 1, 2, 3 and 5 in TIcontacts of the eIFs with the 40S ribosome => structure & function
role of eIF3 and its individual subunits in Met-tRNAi
Met binding as well as in scanning and AUG recognition
role of eIF3 in TI on the Hepatitis Virus C
model organism budding yeast S. cerevisiae
geneticsmolecular biologybiochemistry
How does the cell make sure that all proteins are translated in a timely and spatially coordinated fashion?
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Anna DelijannisováAnna HerrmannováMartina JanoškováOlga JanouškováEdit RutkaiBéla Szamecz Leoš Valášek([email protected])Institute of MicrobiologyAS CR, Prague
Alan G. HinnebuschNICHD, USAPeter Lukavsky,MRC Cambridge, UK Klaus H. NielsenUNI of Aarhus, DKMartin Pospisek,Charles UNI, CRJon LorschJohn Hopkins UNI, USA
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Laboratoř regulace genové exprese Akademie věd České republikyhledá šikovné a pracovité zájemce o bakalářské a magisterské studium!!!
nabízíme: a) zajímavá témata zaobírající se studiem jednoho ze základních procesů genové exprese => regulací proteosyntézy za použití modelového organismu „budding yeast“ Saccharomyces cerevisiae b) špičkově vybavenou laboratoř podporovanou zahraničními grantovými agenturami jako jsou: The Wellcome Trust (UK) Howard Hughes Medical Institute (USA) Fogarty International Center NIH (USA) c) publikační aktivitu v prestižních zahraničních časopisech d) práci v mladém, nadšením sršícím mezinárodním kolektivupožadujeme: a) zvídavého, bystrého, pracovitého a férového ducha b) intermediate english communication skills Kontantní adresa je uvedena níže. Díky,L. ValášekLeoš Valášek, Ph.D.Laboratoř regulace genové expreseMikrobiologický Ústav, AV ČRVídeňská 1083Praha, 142 20Tel: +420 241 062 288Cell: +420 724 731 661Fax: +420 241 062 665e-mail: [email protected]://www.veravalaskova.net/LV.html