Real-time Monitoring technology of Mass spectrometry

Speaker : Achong

Outline

IMS

PTR-MS

SIFT-MS

Application of IMS

IMS-TOF

IMS-IMS-TOF

Application of PTR-MS

PIT-MS

Real-time Monitoring Mass spectrometry

Environmental science

Biochemical and medical diagnosis

Workplace safety

Security check

EI source

Analysis of atmospheric air is especially challenging using electron ionization mass spectrometry, because the gas loading of the ion source by N2 and O2 (as well as water vapor) can be excessive.

70 ev

Real-time Monitoring MS

Soft chemical ionization

Measurement in Real-Time

Without pre-concentration system

I. IMS (ion mobility spectrometer)

Ion mobility mass spectrometry (Eiceman,1991; Baumbach, 2001)

Analytical Chemistry, A390-A397, November 1, 2004

Ion mobility (K)

Journal of Bimolecular Technique, Vol.13, Issue 2, June, 2002

Ion Mobility-Mass Spectroscopy

Witt & Bowers: JACS. 2000, 122, 3458.

Ion mobility mass spectrometry

Advantages:Multiform ion source could be applied.Some isomer could be separated.Real-time measurement

Disadvantages:Lower mass resolutionLower sensitivity to allow accurate analysis of complex mixtures.Standard is requisite

II. PTR-MS (proton-transfer reaction)

Proton-transfer reaction-mass spectrometry (Lagg A, Taucher J, Hansel A, Lindinger W. 1994.)

http://info.uibk.ac.at/c/c7/c722/umwelt/html/animation.html

proton-transfer reaction

H3O+ + R → RH+ + H20

[RH+]

= [H3O+]o(1 - e-k[R]t)

= [H30+]o[R]kt

J. Mass Spectrom Ion Processes, 173 (1998) 191-241

J. Mass Spectrom Ion Processes, 173 (1998) 191-241

J. Mass Spectrom Ion Processes, 173 (1998) 191-241

PTR mass spectrometry

Advantages:Soft ionization and low matrix effectReal-time measurementLow limit of detection (0.5 ppbv for 1-min measurements)

Disadvantages:A large number of VOCs could not be identified and

quantified.

III. SIFT-MS (selected ion flow tube)

http://www.syft.com/

Adams NG, Smith D. 1976. Spanel P ,Smith D. 1995.

SIFT mass spectrometry

Advantages:application of multiple reagent ions (H3O+, NO+ and O2+)quantification in real-time discriminate isomers in Real-Time

Disadvantages:The kinetic parameters of the reagent ion and the analyte ion must be known.

Selected ion flow tube mass spectrometry for no-line trace gas analysis David Smith and Patrik SpanelReference : Mass Spectrometry Reviews, 2005, 24, 661– 700

Application of IMS

IMS/TOF

IMS/IMS/TOF

Ion Mobility/TOFMS

Anal. Chem. 2003, 75,6202-6208

Pulse sequence of IMS & TOF

Anal. Chem. 2003, 75,6202-6208

example spectrum

Analytical Chemistry, 1998 Vol. 70, No. 11, June 1,

Anal. Chem. 2003, 75,6202-6208

collision-induced dissociation

http://proteomics.med.ncku.edu.tw/proteomicsweb/Liao_94_1007.ppt

IMS/IMS/TOF

Anal. Chem. 2006, 78,4161-4174

operational mode A

Anal. Chem. 2006, 78,4161-4174

operational mode B

Anal. Chem. 2006, 78,4161-4174

operational mode C

Anal. Chem. 2006, 78,4161-4174

Application of PTRMS

PIT-MS

PIT-MS

http://cires.colorado.edu/~gouw/Instruments/PITMS/PITMS.html

PIT-MSproton-transfer ion trap-mass spectrometry

J Am Soc Mass Spectrom 2005, 16, 1316–1324

CID process on ion-trap MS

http://www.chem.agilent.com/cag/Other/IonTrap_fig2.gif

calibration measurement of the PIT-MS

J Am Soc Mass Spectrom 2005, 16, 1316–1324

single CID measurement of m/z 59

ambient air pure acetone pure propanal

J Am Soc Mass Spectrom 2005, 16, 1316–1324

CID of propanal and acetone

m/z=59 m/z=41 m/z=31

J Am Soc Mass Spectrom 2005, 16, 1316–1324