racing bacterial cells in microfluidic gradients
DESCRIPTION
Racing Bacterial Cells in Microfluidic Gradients. in order to measure chemotactic efficiency of isogenic bacteria population in correlation to their morphology. Racing Bacterial Cells in Microfluidic Gradients. - PowerPoint PPT PresentationTRANSCRIPT
![Page 1: Racing Bacterial Cells in Microfluidic Gradients](https://reader035.vdocuments.us/reader035/viewer/2022062516/56812a66550346895d8de64b/html5/thumbnails/1.jpg)
Racing Bacterial Cells in Microfluidic Gradients
in order to measure chemotactic efficiency of isogenic bacteria population in correlation to their morphology
![Page 2: Racing Bacterial Cells in Microfluidic Gradients](https://reader035.vdocuments.us/reader035/viewer/2022062516/56812a66550346895d8de64b/html5/thumbnails/2.jpg)
Racing Bacterial Cells in Microfluidic Gradients
in order to measure chemotactic efficiency of isogenic bacteria population in correlation to their morphology
Why:
Length variation is observed in isogenic bacteria population
![Page 3: Racing Bacterial Cells in Microfluidic Gradients](https://reader035.vdocuments.us/reader035/viewer/2022062516/56812a66550346895d8de64b/html5/thumbnails/3.jpg)
Racing Bacterial Cells in Microfluidic Gradients
in order to measure chemotactic efficiency of isogenic bacteria population in correlation to their morphology
Why:
Length variation is observed in isogenic bacteria population
Does length variation have any functional role? → e.g. enhanced/diminshed motility?
![Page 4: Racing Bacterial Cells in Microfluidic Gradients](https://reader035.vdocuments.us/reader035/viewer/2022062516/56812a66550346895d8de64b/html5/thumbnails/4.jpg)
Racing Bacterial Cells in Microfluidic Gradients
in order to measure chemotactic efficiency of isogenic bacteria population in correlation to their morphology
Why:
Length variation is observed in isogenic bacteria population
Does length variation have any functional role? → e.g. enhanced/diminshed motility?
Aim: Physical model of how cell size and number of flagella relate to swimming speeds and efficiency in chemotaxis
![Page 5: Racing Bacterial Cells in Microfluidic Gradients](https://reader035.vdocuments.us/reader035/viewer/2022062516/56812a66550346895d8de64b/html5/thumbnails/5.jpg)
How:
Build microfluidics chamber using PDMS based soft-lithography
![Page 6: Racing Bacterial Cells in Microfluidic Gradients](https://reader035.vdocuments.us/reader035/viewer/2022062516/56812a66550346895d8de64b/html5/thumbnails/6.jpg)
How:
Build microfluidics chamber using PDMS based soft-lithography Create nutrition gradient in chamber to induce chemotaxis (adding sugar)
→ Quantitative measurement of gradient by adding dye in same conc. → Simulating gradient with physics modeling program
chemoattractant bacteria
![Page 7: Racing Bacterial Cells in Microfluidic Gradients](https://reader035.vdocuments.us/reader035/viewer/2022062516/56812a66550346895d8de64b/html5/thumbnails/7.jpg)
How:
Build microfluidics chamber using PDMS based soft-lithography Create nutrition gradient in chamber to induce chemotaxis (adding sugar)
→ Quantitative measurement of gradient by adding dye in same conc. → Simulating gradient with physics modeling program
Recording bacterias with DIC timelapse microscopy Identify single cells and measure their motion tracks (Matlab) as well as size
chemoattractant bacteria
![Page 8: Racing Bacterial Cells in Microfluidic Gradients](https://reader035.vdocuments.us/reader035/viewer/2022062516/56812a66550346895d8de64b/html5/thumbnails/8.jpg)
How:
Build microfluidics chamber using PDMS based soft-lithography Create nutrition gradient in chamber to induce chemotaxis (adding sugar) → Quantitative measurement of gradient by adding dye in same conc.
→ Simulating gradient with physics modeling program Recording bacterias with DIC timelapse microscopy Identify single cells and measure their motion tracks (Matlab) as well as size
![Page 9: Racing Bacterial Cells in Microfluidic Gradients](https://reader035.vdocuments.us/reader035/viewer/2022062516/56812a66550346895d8de64b/html5/thumbnails/9.jpg)
Build microfluidics chamber using PDMS based soft-lithography Create nutrition gradient in chamber to induce chemotaxis (adding sugar) → Quantitative measurement of gradient by adding dye in same conc.
→ Simulating gradient with physics modeling program Recording bacterias with DIC timelapse microscopy Identify single cells and measure their motion tracks (Matlab) as well as size
~1.5 µm thickness~ 3 µm spacing
![Page 10: Racing Bacterial Cells in Microfluidic Gradients](https://reader035.vdocuments.us/reader035/viewer/2022062516/56812a66550346895d8de64b/html5/thumbnails/10.jpg)
![Page 11: Racing Bacterial Cells in Microfluidic Gradients](https://reader035.vdocuments.us/reader035/viewer/2022062516/56812a66550346895d8de64b/html5/thumbnails/11.jpg)
Positions of tracked beads
![Page 12: Racing Bacterial Cells in Microfluidic Gradients](https://reader035.vdocuments.us/reader035/viewer/2022062516/56812a66550346895d8de64b/html5/thumbnails/12.jpg)