questions bank for molecular biology (1)
TRANSCRIPT
-
8/12/2019 Questions Bank for Molecular Biology (1)
1/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
2/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
3/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
4/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
5/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
6/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
7/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
8/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
9/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
10/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
11/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
12/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
13/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
14/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
15/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
16/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
17/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
18/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
19/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
20/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
21/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
22/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
23/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
24/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
25/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
26/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
27/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
28/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
29/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
30/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
31/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
32/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
33/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
34/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
35/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
36/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
37/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
38/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
39/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
40/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
41/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
42/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
43/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
44/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
45/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
46/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
47/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
48/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
49/50
!"#$ &'($)#*+ ,-+. -+/ -$$*0#-)(/ -+$1(2 $"(() 1(2( 0*34#5(/ ,6 7#5'+ 8' -+/ 9-2'+ :-+;-5#0.
-
8/12/2019 Questions Bank for Molecular Biology (1)
50/50
Your labmate was given a piece of DNA and told to make a map of the BamHI restriction enzymesites. The labmate digested the DNA with BamHI to completion and also did a partial digest. Hethen isolated fragments specifically created by the partial digest. These fragments were digestedwith BamHI to completion and the resulting products were run on a new gel. Below are pictures ofthe two gels (note: the size of each band in kilobases is indicated above the band), visualized with
ethidium bromide. Help your labmate interpret the gel by drawing the resulting map. Show therelative location of each of the restriction enzyme sites, the number of nucleotides between the sitesand indicate if the DNA molecule is circular or linear.
BamHI
BamHI
partial
digest
0.5
4.5
2.52.7
5.0
4.5
0.7
2.0
0.7
2.0
0.5
5.0
fragment
0.5
4.5
2.0
0.7
0.5
2.7
fragment
2.5
fragment
3.2
fragment
3.2
7.0
7.0
fragment
2.0 2.0
0.5
0.7
4.5
2.0
0.5
uncut
DNA
7.7