proteins cytoskeleton - ccae sample ppt · overview: protein folding interactions between the amino...
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CCAE Class 1 1
Thurs,April 14
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Protein Structure & Function
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Proteins come in all shapes and sizes
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Proteins 101All proteins found in living organisms are made from the same 20 chemical building blocks (amino acids).
Major types of proteins :Structural Proteins (tubulin, actin, collagen)Enzymes (DNA polymerase, helicase, RNA polymerase, etc.)Transport Proteins (membrane pumps, carrier proteins)Motor Proteins (kinesin, dynein, myosin)
Proteins are major components of all living organisms and the subset of proteins called enzymes perform cellular reactions.
*Proteins are made when an mRNA transcript is translated on a ribosome.
Define: (1) subunit (2) monomer (3) polymerization
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Amino AcidsAll proteins found in nature are made from the same 20 building blocks
These building blocks are called amino acids.*
Amino acids all have the same chemical structure with the exception of their side chain, aka ‘R-group’.
Each amino acid’s side chain has specific properties. Side chains can be either: negative, positive, polar uncharged (hydrophilic), or nonpolar (hydrophobic).
N’ end C’ end(amino end) (carboxylic acid end)
The interactions between many amino acids determine protein shape and function.
*Amino acids are also called residues or peptides. What is a polypeptide?
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Three-Letter Abbreviation
One-Letter Abbreviation
hydrophilic hydrophobic
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Peptide BondsHow do amino acids attach to each other to make a peptide chain?:
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PolypeptideAll of these amino acids are connected by peptide bonds, forming a polypeptide.When the polypeptide folds into its proper shape, it is called a protein.
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Protein FoldingInteractions between the amino acids determine how polypeptides fold.
SIDE CHAIN INTERACTIONS
“BACKBONE” INTERACTIONS (non side chain)
hydrophobic
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Overview: Protein FoldingInteractions between the amino acids determine how the polypeptide folds.Protein conformation refers to the proteins specific shape, based on this folding.
POLAR amino acid side chains (neutral charge) can make hydrogen bonds with other POLAR amino acid side chains.
NON-POLAR amino acid side chains can interact with other NON-POLAR amino acid side chains by hydrophobic forces.
NEGATIVE side chains can make ionic bonds with POSITIVE side chains, or can repel other NEGATIVE side chains (and visa versa).
ANY amino acid can form hydrogen bonds with another in close proximity.
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Protein Folding:Proteins always fold to keep nonpolar (hydrophobic) side chains protected from the outside environment.
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There are four types of structures that proteins can make:Primary, Secondary, Tertiary, and Quaternary.
Primary structure of a proteinis the chain of amino acids connectedby peptide bonds.
Amino acids are numbered, starting at the Amino end (N’).
Protein Primary Structure (1º)
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Protein Secondary Structure (2º)There are four types of structures that amino acids can be found in:
Primary, Secondary, Tertiary, and Quaternary.
Secondary structures are formed when the “backbone” of the polypeptide interacts by hydrogen bonds (secondary structures do not include form from side chain interactions).
Three common secondary structures: alpha-helix, beta-sheet, and coils.
COIL
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Alpha Helix
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Beta-Sheet
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Analyze this.
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Protein Tertiary Structure (3º)Tertiary structure of proteins is the combination of multiple secondary structures into 3D domains.
Domains can form their shapes independently.
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Protein Quaternary Structure (4º)Quaternary Structure: The structure of a protein that results from the interaction of two or more individual polypeptides to give larger functional proteins
Examples: hemoglobin, actin filament
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First Look at Mutations
A single amino acid change in a protein can have major consequences!
[?] How may mutations affect a protein’s conformation?
Example: Sickle Cell Anemia
Hemoglobin is a quaternary protein made up of four subunits twisted together, each of which contains a heme group, capable of transporting a molecule of oxygen.
Sickle cell anemia is a disorder that results from a mutated hemoglobin protein
Glutamic acid (E) at position 6 of hemoglobin à Valine (V)
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CCAE Class 1 20
PART TWO
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Cells are very animated!
Questions:•What makes up the structural support of a cell?• How do materials get transported within and between cells?• How do cells move?
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What’s the purpose of a skeleton? What would happen if we didn’t have skeletons?
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Cells contain elaborate arrays of protein fibers that function in:establishing cell shapeproviding mechanical strengthCELL locomotionchromosome separation in cell divisionintracellular transport of organelles
Cytoskeleton controls movement and structure
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17_02_3 types of protein filaments
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There are three major classes of cytoskeleton proteins.
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Size: 10 nm in diameter
Main functions: support within cellssupport between cellselastic, tensile strength
I. Intermediate Filaments
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Many cells need to stick together
(NO NEED TO MEMORIZE)
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Size: 7 nm in diameter
Subunit: G-Actin +ATP
Main functions: cell strengthcell locomotioncell division
Polarity: plus and minus ends
II. Actin
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II. Actin
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MINUS PLUS
Polarity of Actin
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III. Microtubules
Microtubules are the tracks on which cellular cargo is transported.
MTs are scaffolding and support for the cell.
Structure: Microtubules are straight, hollow cylinders whose wall is made up of a ring of 13 smaller ‘proto’filaments.
Size: 25 nm in diameter
Subunit: MTs are built by the assembly of dimers of alpha tubulin and beta tubulin..
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Microtubule Polarity
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The MT end attached to the centrosome is called the minus end; the free end is the plus end.
Microtubules grow at the plus end by the polymerization of GTP-bound tubulin dimers , and shrink by the release of GDP-bound tubulin dimers. ”Catastrophe” and “Rescue”
Beta-tubulin faces the + end.Alpha-tubulin faces the – end.
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Microtubules are very dynamic!
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https://www.youtube.com/watch?v=tJKXNarWpqE
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Cellular trafficking: Second look
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How do materials get transported throughout the cell?
There are two major groups of MOTOR PROTEINS that travel on microtubules:
•kinesins (move toward plus end of the microtubules)•dyneins (move toward the minus end).
Example:The rapid transport of organelles, like mitochondria, along neurons takes place along microtubules with their plus ends pointed toward the end of the neuron.
Molecular Motors
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Molecular Motors
There are two major groups of MOTOR PROTEINS that travel on microtubules:
•kinesins (move toward plus end of the microtubules)•dyneins (move toward the minus end).
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Kinesin and ATP
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Motor Proteins Up Close: Kinesin
Kinesin Video
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Flagellar Motion: Live Demo
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Microtubules are responsible for cell movement.
Cilia and Flagella
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39WATCH THIS: http://csls-text.c.u-tokyo.ac.jp/active/06_01.html
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Cytoskeleton & Motor Proteins in LiteratureEXTRA SLIDE
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Question 1: How do organelles and vesicles get transported around the cell?
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Molecular Motors: Lab Experiment
What kind of animal cell should we use?
What can you see from the image?
EXTRA SLIDE
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Molecular Motors: Experiment
Question 2: What are these “tracks” that the vesicles and organelles move on?
Hypothesis: The “tracks” that organelles and vesicles move on are microtubules.
How would you identify a microtubule? Hint: what can you stain specifically?Tubulin!
Question 3: What is the energy sourcerequired for this activity?
Experiment: Deplete all ATPAdd in ATP and see what happensUse a negative control
-ATP + ATP
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EXTRA SLIDE
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Molecular Motors: Experiment
4. What are the proteins that help cell transport and movement?
Hypothesis: kinesin protein?
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EXTRA SLIDE
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End of Class 3
CCAE Class 1 44
Homework for Thursday, April 21:
WATCH all YouTube links in this slideshow
REVIEW slides from class, and organize notes
COMPLETE Cytoskeleton Worksheet and Protein Structure Activity