program - eupa 2019 · of complex proteomics data, in a hands on workshop performed by experts in...

Potsdam 2019

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PROTEOMIC FORUM 2019XIII. Annual Congress of the European Proteomics Associati on:From Genes via Proteins and their Interacti ons to Functi ons

March 24–28

PROGRAM

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general InformaTIon

See the bar raised at thermofisher.com/TMT

For Research Use Only. Not for use in diagnostic procedures. © 2018 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. AD65161-EN 0218M

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The proteome is complex and dynamic. You need innovative tools to study the sophistications of Mother Nature. TMT multiplexed solutions enable you to ask real questions of your sample and obtain a meaningful description of how protein expression changes with disease, with the progression of time, and even within different parts of the cell. TMT workflows open the door to answering a whole new set of questions quickly.

proteomics






proteomics

3

Table of conTenTs

Welcome note ......................................................................................................................... 4

Organization and imprint ........................................................................................................ 5

Program overviews Sunday, March 24 ............................................................................................................. 6 Monday, March 25 ........................................................................................................... 7 Tuesday, March 26 ............................................................................................................ 8 Wednesday, March 27 ...................................................................................................... 9 Thursday, March 28 .......................................................................................................... 9

Scientific program Sunday, March 24 ............................................................................................................. 10 Monday, March 25 ........................................................................................................... 12 Tuesday, March 26 ............................................................................................................ 20 Wednesday, March 27 ...................................................................................................... 30 Thursday, March 28 .......................................................................................................... 37

Poster sessions Poster session 1 I Poster session 3 ................................................................................. 38 Poster session 2 I Poster session 4 ................................................................................. 52

General information ................................................................................................................ 66

Awards and grants .................................................................................................................. 67

Room overview and booth plan ............................................................................................. 68Sponsors and exhibitors l Media cooperations .................................................................... 69

Social program ........................................................................................................................ 70

Index of presenting authors and session chairs ..................................................................... 71

Key of abstract IDsIL Invited lectureNC News cornerP Poster presentationSP Short presentation






proteomics






proteomics

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Welcome noTe

Dear Sir or Madam, Dear colleague,

On behalf of the organizing committee it is a great pleasure to invite you to join the Proteomic Forum 2019/ XIII. Annual Congress of the European Proteomics Association: From Genes via Proteins and their Interactions to Functions.

The congress will provide a unique opportunity to present and discuss progress in the inter-disciplinary field of proteome research, including applications. In Potsdam in the vicinity of Sanssouci Palace you will have the chance to discuss your data with leading experts. Recognizing current bottle necks, a special emphasis will be placed on options for analysis of complex proteomics data, in a hands on workshop performed by experts in the field on Thursday as well as in special sessions by vendors in which dedicated software solutions for data analysis will be presented. Education will be a major theme of the conference and thus the program will feature diverse activities ranging from an Educational Day on Sunday, two News Corners and a EuPA Young Investigator Prize Session and Challenge Session to a Career Session.From Monday through Wednesday, a mix of plenary and parallel sessions will cover topics like: Systems Biology – Multi-omics Approaches, Proteomics in Cell Biology, Protein-MS in Clinical Applications, Structural Proteomics/ Protein Complexes, Multi Attribute Monitoring, From Data to Information to Knowledge to Utility, Interactomics, Bioinformatics: From Data Analysis to Integration, Human Diseases, Redox Proteomics, Microbiome and Microbial Patho-gens, Plant and Nutrition, New Technologies, Signaling Networks/ Pathway Activity, Protein Transport and Subcellular Localisation, Antibiotic Resistance Mechanisms/ New Antimicrobial Compounds, Host-Pathogen Interactions, Proteogenomics and Clinical Applications.

Thus, a broad spectrum of interdisciplinary research and applications of proteomics will be covered by talks of invited speakers and short talks selected from submitted abstracts. Each of the participants will have the opportunity to present latest results in four poster sessions from Sunday through Wednesday.Complementing the scientific program, an industrial exhibition will cover the latest products and developments in proteomics technologies.You are cordially invited by the German Society of Proteome Research and the European Proteomics Association.

We are delighted to welcome you in Potsdam!

With best regards,

Albert Sickmann and Uwe Völker

Session chairs & pre-conference organizersThe organizers of the Proteomic Forum 2019/ XIII. EuPA Congress wish to thank all those who have been actively involved in the organization of the scientific program as pre-conference program organizer, reviewer or session chair. Their involvement is highly appreciated and has greatly contributed to the quality of the final program.

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organIzaTIon anD ImPrInT

congress venueUniversity of Potsdam I Campus Griebnitzsee, Haus 6August-Bebel-Strasse 89 I 14482 Potsdam, DE

Hosting societiesEuropean Proteomics Association (EuPA) www.eupa.org

in cooperation withGerman Society of Proteome Research (DGPF e. V.) www.dgpf.org

conference chairsProf. Dr. Uwe VölkerUniversity Medicine GreifswaldInterfaculty Institute for Genetics and Functional Genomics I Greifswald, DE

Prof. Dr. albert sickmannLeibniz-Institute for Analytical Sciences – ISAS – e. V. I Dortmund, DE

Organizing committeeAndrea Urbani (Rome/IT)Julia Bandow (Bochum/DE)Dörte Becher (Greifswald/DE)Hans-Peter Braun (Hannover/DE)Tilman Grune (Potsdam/DE)Stefanie Hauck (Munich/DE)Bernhard Küster (Munich/DE)Katharina Riedel (Greifswald/DE)Kai Stühler (Duesseldorf/DE)Marius Ueffing (Tuebingen/DE)Bettina Warscheid (Freiburg/DE)

Conference organizerConventus Congressmanagement & Marketing GmbHCyntia Rammel, Carolin RollnikPhone +49 3641 31 16-333/-352 I [email protected]

Design/layoutLayout krea.tif-studio UG (haftungsbeschränkt)Print siblog – Gesellschaft für Dialogmarketing, Fulfillment & Lettershop mbHCirculation 550Editorial deadline March 15, 2019

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Program oVerVIeWsunday, march 24

H03 S23 S24 S25 1st level Foyer10:00–11:30

11:45–13:15

13:45–15:15

15:30–16:45 15:30–16:45 15:30–16:45

17:00–18:00

18:05–18:15Meet the industry 18:15–20:15 18:15–19:45

EuPA Young Proteomics Investigator Club (YPIC)



Welcome and opening lecture

Poster session 1Even poster numbers

Meet the experts

Meet the experts

Meet the experts

Welcome reception

March 24

p. 10

p. 10

p. 10

p. 10 p. 10 p. 10

p. 11

p. 11

p. 11

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Program oVerVIeWmonday, march 25

H01 H02 H03 H04 1st level08:30–10:00

10:30–12:00

12:15–13:15 12:15–13:15

13:30–14:30 13:30–14:30 13:30–14:30

14:30–15:30 14:30–16:30

15:30–16:30

16:40–17:40 16:40–17:40 16:40–17:40 16:40–17:40

17:45–18:45General assembly DGPF

Training course 1 Thermo Fisher Scientific GmbH

News corner 1

Lunch workshop Thermo Fisher Scientific

Plant and nutrition

Proteomics in cell biology

Interactomics: Affinity, proximity and spatial [...]

Training course 2Waters GmbH

Human disease: Cancer, neuro, cardio, rare, immunology

Bioinformatics: From data analysis to integration

Poster session 2Odd poster numbers

Lunch workshopBruker Daltonik

Multi attribute monitoring, host cell protein, [...]

March 25

Structural proteomics:Protein complexes

Protein‐MS in clinical applications

p. 12

p. 12

p. 13 p. 13

p. 13 p. 14p. 14

p. 15

p. 16

p. 16 p. 16p. 18

p. 18

p. 15

p. 17

March 28H01 H02 H03 H04 1st level H03

08:30–10:00 09:30–17:00

10:00–11:00

10:30–12:00

12:15–13:15

13:15–14:4513:30–14:30 13:30–14:30

14:30–16:3014:45–15:45

15:30–16:30

16:40–17:40 16:40–17:40 16:40–17:40

17:45–19:15

Key

Closing and award lecture

March 27

Educational workshop Tool‐training for proteomics

Proteogenomics and clinical applications

Career session

Training course 8 Tecan Deutschland

Technological Innovations

Signaling networks, pathways and pathway activity (PTMs)


Antibiotic resistance mechanisms [...]

Interactomics: Affinity, proximity, and spatial proteomics

Protein transport and subcellular localization

Host‐pathogen interactions

Training Course 6 Bruker Daltonik

Lunch workshop SERVA Electro‐phoresis

Training course 7SERVA Electro‐phoresis

Workshop | Training course

SymposiumPlenary sessionPoster sessionNetworking

Lunch workshop | Industry sessionEuPA/DGPF session

p. 30

p. 31

p. 30

p. 31

p. 32p. 33p. 32

p. 34

p. 34

p. 34

p. 35p. 35p. 36

p. 37

p. 37

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Program oVerVIeWTuesday, march 26

H01 H02 H03 H04 1st level08:30–10:00

10:30–11:30 10:30–12:00

12:15–13:15 12:15–13:15 12:15–13:15

13:20–14:2013:30–14:30 13:30–14:30 13:30–14:30

14:30–15:30 14:30–16:30

15:30–16:30

16:40–17:40 16:40–17:40 16:40–17:40

17:50–18:10YPIC‐Challenge

Congress party(Biosphäre)

Systems biology:Multi‐omics approaches

Proteomics in cell biology

Lunch workshop Thermo Fisher Scientific

Redox proteomics

Lunch workshop Waters

New technologies

Training course 5AB SCIEX Germany

EuPA Young Investigator Prize session

Lunch workshop Evosep

Human disease: Cancer, neuro, cardio, rare, immunology

News corner 2

Training course 4Bruker Daltonik

Poster session 3Even poster numbers

Microbiome and microbial pathogens

March 26

From data to infor‐mation to know‐ledge to utility

Training course 3Thermo Fisher Scientific

19:30–23:59

p. 28

p. 20

p. 20

p. 20

p. 21 p. 22

p. 22 p. 23p. 24

p. 22

p. 26

p. 26

p. 26

p. 27

p. 28 p. 27

p. 24

p. 23

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Program oVerVIeWWednesday, march 27 and Thursday, march 28

March 28H01 H02 H03 H04 1st level H03

08:30–10:00 09:30–17:00

10:00–11:00

10:30–12:00

12:15–13:15

13:15–14:4513:30–14:30 13:30–14:30

14:30–16:3014:45–15:45

15:30–16:30

16:40–17:40 16:40–17:40 16:40–17:40

17:45–19:15

Key

Closing and award lecture

March 27

Educational workshop Tool‐training for proteomics

Proteogenomics and clinical applications

Career session

Training course 8 Tecan Deutschland

Technological Innovations

Signaling networks, pathways and pathway activity (PTMs)


Antibiotic resistance mechanisms [...]

Interactomics: Affinity, proximity, and spatial proteomics

Protein transport and subcellular localization

Host‐pathogen interactions

Training Course 6 Bruker Daltonik

Lunch workshop SERVA Electro‐phoresis

Training course 7SERVA Electro‐phoresis

Workshop | Training course

SymposiumPlenary sessionPoster sessionNetworking

Lunch workshop | Industry sessionEuPA/DGPF session

p. 30

p. 31

p. 30

p. 31

p. 32p. 33p. 32

p. 34

p. 34

p. 34

p. 35p. 35p. 36

p. 37

p. 37

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scIenTIfIc Programsunday, march 24

10:00–11:30 session 1Room H03 EuPA Young Proteomics Investigator Club (YPIC)Chairs M. Langini , N. Schmidt (Duesseldorf/DE)

10:00 Omics integration strategies C. Pineau (Rennes/FR)

10:45 Cross-linking/Mass spectrometry for analyzing protein assemblies A. Sinz (Halle a. d. Saale/DE)

11:30–11:45 Coffee break within the industrial exhibition

11:45–13:15 session 2Room H03 EuPA Young Proteomics Investigator Club (YPIC)Chairs A. Hogrebe, M. Langini (Duesseldorf/DE)

11:45 Application of thermal proteome profiling M. Bantscheff (Heidelberg/DE)

12:30 Methods to interrogate the subcellular location of proteins K. Lilley (Cambridge/GB)

13:15–13:45 Lunch break within the industrial exhibition

13:45–15:15 session 3Room H03 EuPA Young Proteomics Investigator Club (YPIC)Chairs A. Banaei Esfahani (Zurich/CH), D. Rešetar Maslov (Rijeka/HR)

13:45 Analyzing protein turnover using SILAC M. Selbach (Berlin/DE)

14:30 An inclusive future for proteomics: adding: PTMs to the routine recipe L. Martens (Ghent/BE)


15:30–16:45 Meet the expertsRoom S23–S25 EuPA Young Proteomics Investigator Club (YPIC)

Room S23 Chair: D. Resetar Maslov (Rijeka/HR) Experts: L. Martens (Ghent/BE), M. Bantscheff (Heidelberg/DE) C. Pineau (Rennes/FR)

Room S24 Chair: M. Langini (Duesseldorf/DE) Experts: S. Pennington (Dublin/IE), K. Lilley (Cambridge/GB) M. Babu (Regina/CA)

Room S25 Chair: F. Wiesenhofer (Klosterneuburg/AT) Experts: A. Sinz (Halle a. d. Saale/DE), N. Kelleher (Evanston, IL/US) J. Yates (La Jolla, CA/US)

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scIenTIfIc Programsunday, march 24

17:00–18:00 Welcome and opening lectureRoom H03

Chair U. Völker (Greifswald/DE)

Proteomics in translational research M. Mann (Martinsried/DE)

18:05–18:15 meet the industryRoom H03

18:15–20:15 Poster session 11st level

Posters Even poster numbers Please refer to page 38 for further information.

18:15–19:45 Welcome receptionFoyer Please refer to page 70 for further information.

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scIenTIfIc Programmonday, march 25

08:30–10:00 Plenary session 1Room H03 Structural proteomics I Protein complexesChair B. Warscheid (Freiburg/DE)

08:30 Developing the orbitrap for native top-down proteomics and high-fidelityIL1 readout of endogenous protein complexes N. Kelleher (Evanston, IL/US)

09:00 Mitochondrial network organization during neuronal reprogrammingIL2 M. Babu (Regina/CA)

09:30 SWATH-MS profiling of signalosomes during activation and checkpoint IL3 inhibition in primary T cells J. Celis-Gutierrez (Marseille/FR), P. Blattmann (Zurich/CH), Y. Zhai N. Jarmuzynski, K. Ruminski, C. Gregoire, Y. Ounoughene F. Fiore (Marseille/FR), S. Hauri (Zurich/CH), E. Caron (Montreal/CA) R. Aebersold (Zurich/CH), R. Roncagalli, B. Malissen (Marseille/FR) M. Gstaiger (Zurich/CH)


10:30–12:00 Plenary session 2Room H03 Protein-MS in clinical applicationsChair A. Sickmann (Dortmund/DE)

10:30 Mass spec (discovery and targeted) to advance basic and translationalIL4 cancer research H. Rodriguez (Bethesda, MD/US)

11:00 Implementing precision health: Targeting patients at risk for major adverseIL5 cardiac events J. van Eyk (Los Angeles, CA/US)

11:30 Development and delivery of advanced protein biomarker tests for an era ofIL6 precision medicine S. Pennington (Dublin/IE)


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12:15–13:15 Lunch workshop 1 I Bruker Daltonik GmbHRoom H03 Unleashing the power of TIMS and QTOF technology with the unique PASEF acquisition mode

Trapped ion mobility spectrometry (TIMS) in combination with high-reso-lution QTOF mass spectrometer has been shown to provide a number of benefits for shotgun proteomics experiments. The TIMS-QTOF technology provides new acquisition strategy such as data-dependent acquisition Parallel Accumulation – Serial Fragmentation (PASEF) and data-independent PASEF (diaPASEF) which will both be presented at our lunch seminar.

Introduction to the timsTOF Pro powered by PASEFS. Koch (Bruker Daltonik GmbH/DE)

PASEF scan modes on the timsTOF Pro for rapid proteome analysis M. Mann (Max Planck Institute of Biochemistry/DE)

12:15–13:15 Lunch workshop l Thermo Fisher Scientific GmbH Room H04 MS toolbox for structural and functional biology

In this seminar, we will share several exciting new advancements in instru-mentation and methods to study protein complex assemblies and structures as well as generate standardized proteome profiles which meet the require-ments of translational research projects.

Comprehensive proteome mapping using LC-FAIMS Pro-MS/MSS. Memmi (Thermo Fisher Scientific)

MS analyses of glycosylated proteins and DNA-protein complexesH. Urlaub (Max-Planck-Institute for Biophysical Chemistry, Goettingen)

Clinical proteotype analysis: From panel sequencing to deep proteotype sequencingB. Wollscheid (ETH Zurich)

13:30–14:30 Symposium 1Room H03 Multi attribute monitoring, host cell protein, antibody drug conjugates, target identificationChair B. Wollscheid (Zurich/CH)

13:30 Multi-attribute monitoring of antibody modifications in a quality control IL7 laboratory: Points to consider D. Reusch (Penzberg/DE)


14


13:55 Candidate optimization for antibody-drug conjugatesIL8 R. Kellner (Darmstadt/DE)

14:20 Evaluation of a high-resolution/accurate-mass analyser to characterize andSP1 quantify host cell proteins N. Pythoud, J. Bons (Strasbourg/FR), G. Husson (Zwijnaarde/BE) S. Cianferani, C. Carapito (Strasbourg/FR)

13:30–14:30 Symposium 2Room H04 Plant and nutritionChair H.-P. Braun (Hannover/DE)

13:30 The contribution of autophagy to plant protein turnover ratesIL9 H. Millar (Perth/AU)

13:55 Combining linear motif analysis and kinase expression profiling for SP4 kinase-target assignment J. Mergner, M. Frejno (Freising/DE), S. Altmann P. Falter-Braun (Neuherberg/DE), B. Kuster (Freising/DE)

14:07 Proteomic analysis of goat milk kefir: Profiling the fermentation-time SP2 dependent protein digestion and identification of potential peptides with biological activity J. J. Izquierdo-González, F. Amil-Ruiz, C. Fuentes-Almagro M. J. Rodríguez-Ortega (Córdoba/ES)

14:19 Food proteomics: Tuna species differentiation using a LC-HRMS/SP3 MS method and peptide biomarkers I. Brümmer, J. Brockmeyer (Stuttgart/DE)

13:30–14:30 Symposium 3Room H01 Interactomics: Affinity, proximity, and spatial proteomicsChair S. Hauck (Munich/DE)

13:30 Thermal proteome profiling identifies candidate targets of a novel small SP 5 molecule that exploits vulnerability to reactive oxygen species in lung cancer F. Johnson, A. Liu, D. Lu, A. Nagelberg, A. Prudova, J. Luu Y. Inoue (Vancouver/CA), R. Somwar (New York, NY/US), P. Sorensen G. Morin, W. Lockwood (Vancouver/CA)

13:42 Multilayered proteomic interaction analysis identifies cancer mutations SP6 that affect the composition and function of kinase complexes M. Mehnert, R. Ciuffa, F. Frommelt, F. Uliana, A. van Drogen, M. Gstaiger R. Aebersold (Zurich/CH)

15


13:54 Dissecting the role of Lebercilin in cilia disassemblySP7 K. Boldt, M. Moniruzzaman, T. Leonhard, S. Bolz, K. Junger, A. Cakar W. Haq, T. Beyer, M. Ueffing (Tuebingen/DE)

14:06 Peptide array protein pulldowns and BioID unravel SLiM based protein SP8 interactions in the transcription factor C/EBP α E. Ramberger, D. Perez-Hernandez, V. Sapozhnikova, K. Zimmermann E. Kowenz-Leutz, P. Mertins (Berlin/DE), G. Dittmar (Luxembourg/LU) A. Leutz (Berlin/DE)

14:18 Quantitative phosphoproteomics reveals an extended basophilic kinase SP9 substrate motif for modulating protein interactions and dynamics by dual-site phosphorylation L. Reimann, A. Schwaeble (Freiburg/DE), Y. Leber (Bonn/DE) A. L. Fricke (Freiburg/DE), S. Schaeuble (Jena/DE) H. Wiese (Freiburg, Ulm/DE), M. Puchinger (Vienna/AT) C. Peikert (Freiburg/DE), P. van der Ven (Bonn/DE), G. Radziwill (Freiburg/DE) K. Djinović-Carugo (Vienna/AT; Freiburg/DE), D. Fuerst (Bonn/DE) B. Warscheid (Freiburg/DE)

14:30–16:30 Poster session 21st level

Posters Odd poster numbers Please refer to page 52 for further information.

14:30–15:30 Training course 1 I Thermo Fisher Scientific GmbHRoom H02 Latest advances in multiplexing technology

Thermo ScientificTM Tandem Mass Tags (TMT) reagents enable deep, quan-titative proteome profiling – With the added benefit of a 10X gain in sample throughput. Multiplexed isobaric tags can be used for wide variety of samples providing exceptional analytical depth, high throughput, reproducibility, with few missing quantitative values. In this workshop, our expert speakers will discuss the benefits of performing multiplexed quantification, its combination with FAIMS and a new TMT standard which can be used to rigorously bench-mark Orbitrap system performance.

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15:30–16:30 Training course 2 I Waters gmbHRoom H01 Progenesis QI Proteomics – Software for discovery omics analysis:Identify and quantify all your data with confidence

Progenesis QI software – Discover the significantly changing proteins in your samples. Progenesis QI Proteomics software enable you to accurately quan-tify and identify the proteins in your samples that are significantly changing. Most common identification search engines are supported. But you can also use public/private spectral libraries and create easily and confidently your own spectral library. With support for all common vendors’ raw formats and a guided workflow, Progenesis QIp help to overcome your Omics analysis challenges.

16:40–17:40 Symposium 4Room H03 Bioinformatics: From data analysis to integrationChair G. Tsangaris (Athens/GR)

16:40 A time of enlightenment: Straightforward identification of protein IL10 modifications L. Martens (Ghent/BE)

17:05 Integrating database search and de novo sequencing for SP10 immunopeptidomics with DIA approach B. Shan, W. Zhang, H. Tran, L. Xin, X. Chen, M. Li (Waterloo/CA)

17:17 Simple targeted assays for metabolic pathways and signaling: SP11 A powerful tool for targeted proteomics D. Kopczynski, A. Hentschel, C. Coman (Dortmund/DE) N. H. Schebb (Hannover, Wuppertal/DE), T. Hornemann (Zurich/CH) D. Mashek (Minneapolis, MN/US), H.-F. Schött, K. Lorenz (Dortmund/DE) C. S. Ejsing (Odense/DK, Heidelberg/DE), J. Medenbach (Regensburg/DE) R. Ahrends (Dortmund/DE)

17:29 DeepRibo: A neural network for the precise gene annotation of SP12 prokaryotes by combining ribosome profiling signal and binding site patterns J. Clauwaert, G. Menschaert, W. Waegeman (Ghent/BE)

16:40–17:40 Symposium 5Room H04 Proteomics in cell biologyChair K. Stühler (Duesseldorf/DE)

16:40 Ubiquitinome dynamics upon proteasome modulationSP13 J. Demmers (Rotterdam/NL)

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16:52 Proteome analyses of the human hematopoietic stem cell niche SP14 reveal molecular features of age-dependent functional decline M. L. Hennrich, N. Romanov, P. Horn (Heidelberg/DE) S. Jaeger (Barcelona/ES), P. Bork (Heidelberg/DE), P. Aloy (Barcelona/ES) A. Ho, A.-C. Gavin (Heidelberg/DE)

17:04 In-depth proteomic characterization of lineage-biased hematopoietic SP15 progenitor cells in the fetus and adult M. Jassinskaja, K. Pimková, E. Johansson, E. Sitnicka, J. Hansson (Lund/SE)

17:16 Using crosslinking coupled to mass spectrometry to study structural SP16 dynamics of enzyme-substrate complexes C. Sailer, F. Offensperger, A. Julier, K.-M. Kammer (Konstanz/DE) R. Walker-Gray (Konstanz/DE, London/GB), M. G. Gold (London/GB) M. Scheffner, F. Stengel (Konstanz/DE)

17:28 The prion protein: From interactions through evolution and function to SP17 avenues for disease intervention G. Schmitt-Ulms, M. Mehrabian, D. Williams, F. Ghodrati X. Wang (Toronto/CA)

16:40–17:40 Symposium 6Room H01 Human disease: cancer, neuro, cardio, rare, immunologyChair A. Urbani (Rome/IT)

16:40 Top-down proteomics in cardiac disease and regenerationIL11 Y. Ge (Madison, WI/US)

17:05 pwSILK: In vivo large scale mapping of protein turnover in the human SP18 cerebrospinal fluid J. Colinge, S. Lehmann, J. Vialaret, C. Hirtz, E. Cornillot M. Ory (Montpellier/FR)

17:17 Alanyl-glutamine improves cell viability and cytoprotective responses in SP19 endothelial cells during peritoneal dialysis fluid exposure R. Herzog, S. Tarantino (Vienna/AT), M. Bartosova C. P. Schmitt (Heidelberg/DE), C. Aufricht, K. Kratochwill (Vienna/AT)

17:29 Proteome-level response profiling of TRAIL-based combination therapies SP20 in NSCLC S. Surinova, A. Bhamra, N. Angelopoulos, I. Areso, A. Montinaro H. Walczak (London/GB)

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16:40–17:40 news corner 1Room H02 Chair F. Lottspeich (Stockdorf/DE)

16:40 Thermal proteome profiling of dosed animals measures target and off-NC1 target engagement T. Werner, J. Perrin, N. Kurzawa, D. Childs, M. Kalxdorf, A. Rutkowska D. Poeckel, E. Stonehouse, K. Strohmer, B. Heller, D. Thomson, J. Vappiani J. Krause, H. C. Eberl, C. Rau, H. Franken, W. Huber, M. Faelth-Savitski S. Mikhail, M. Bantscheff, G. Bergamini (Heidelberg/DE)

16:52 Benchmarking PEA-Olink technology with mass spectrometry-based NC2 protein quantifications in blood plasma A. Petrera, C. Von Toerne, J. Behler, C. Huth, B. Thorand, F. Metzger C. Gieger, S. Hauck (Munich/DE)

17:04 Veterinary proteomics: An unified approach for multiple species, sample NC3 types and mixed pathophysiology A. Horvatić, J. Kuleš, N. Guillemin, A. Galan, V. Mrljak (Zagreb/HR) P. D. Eckersall (Zagreb/HR; Glasgow/GB)

17:16 DB-nESI overcomes the ion flux problem of modern nanoLC-MS/MSNC4 S. Brandt, I. Reginskaya, M. Schilling (Dortmund/DE) A. Sickmann (Dortmund, Bochum/DE; Aberdeen/GB) R. P. Zahedi (Montreal/CA), J. Franzke, S. Loroch (Dortmund/DE)

17:28 Sequential Digest + XiSearch + XiFDR: More bang for your buck in NC5 crosslinking mass spectrometry L. Fischer (Berlin/DE; Edinburgh/GB), M. Mendes (Berlin/DE) J. Rappsilber (Berlin/DE; Edinburgh/GB)

17:45–18:45 general assembly DgPfRoom H03

PUBS: American LaboratoryAmerican Drug DiscoveryAmerican Pharmaceutical ReviewBio BusinessBio IT WorldBioTech WorldBioTechnology FocusBioPharm InternationalBioProcess InternationalChemical & Engineering NewsDrug Discovery and DevDrug Discovery NewsEnvironmental Science & Technology

Food ProcessingFood QualityFood Safety MagazineGenetic Engineering News (GEN)Genome TechnologyGenomics & ProteomicsJour of The American Soc for Mass SpectrometryLab Asia Media GuideLab Business - JesmarLCGC AsiaLCGC N. AmericaLCGC EuropeMolecular and Cellular Protemomics Nature Methods

Pharmaceutical Discovery & DevelopmentPharmaceutical ManufacturingPharmaceutical TechnologyPharmaceutical Technology EUROPEPharmaceutical ExecutivePharm Form and QualityProteomics JournalScientific AmericanScientific Computing & InstrumentationScientific Computing World

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08:30–10:00 Plenary session 3Room H03 Systems biology – Multi-omics approachesChair E. Csosz (Debrecen/HU)

08:30 Unravelling the hidden universe of small proteins in bacterial genomesIL12 L. Serrano, S. Miravet, M. Lluch (Barcelona/ES)

09:00 Proteomics gone fast – And what to do with itIL13 M. Ralser (Berlin/DE)

09:30 The in silico human surfaceome & technologies for the elucidation of IL14 the Surfaceome nanoscale organization B. Wollscheid (Zurich/CH)


10:30–11:30 Training course 3 I Thermo Fisher Scientific GmbHRoom H02 Thermo Scientific MS Toolbox for structural biology

To understand protein function and mechanism of action, it is essential to determine protein complex assembly and structure. Thermo Fisher Scientific leads the way in accelerating protein structure-function studies with its Inte-grative Structural Biology solutions. Thermo ScientificTM Orbitrap-based mass spectrometers enable characterization of intact proteins and large complexes leading to understanding dynamic structure-function relationships. In this workshop, our expert speakers will share practical aspects of performing structural proteomics via native and top-down MS.

10:30–12:00 Plenary session 4Room H03 Proteomics in cell biologyChair S. Pennington (Dublin/IE)

10:30 Capturing the spatial organisation of the transcriptome and proteomeIL15 K. Lilley (Cambridge/GB)

11:00 Protein stability in drug discovery and molecular biologyIL16 M. Savitski (Heidelberg/DE)

11:30 The testicular proteome: Insights into normal and pathological IL17 spermatogenesis C. Pineau (Rennes/FR)

scIenTIfIc ProgramTuesday, march 26

21



12:15–13:15 Lunch workshop l Waters GmbHRoom H01

A Multi-Function Cyclic Ion Mobility – Mass Spectrometry System We describe a novel system that integrates cyclic Ion Mobility (cIM) into a tandem MS platform with the latest technology enhancements from Waters. The combination of these elements provides unparalleled IMS performance and experimental flexibility. The instrument is a cyclic ion mobility-enabled quadrupole time-of-flight (Q-cIM-oaToF) mass spectrometer. The circular path of the mobility device (one metre path length) minimizes instrument footprint whilst providing a longer, higher mobility resolution separation path; a multi-pass capability provides significantly higher resolution over a reduced (selected) mobility range. In addition to standard operational modes of the system, such as single and multi-pass cIM separation, the ‘multi-function’ instrument design enables a range of multi-stage experiments to be performed such as: ion selection of specific mobility separated species, ion storage, activation, IMSn and importantly, custom combinations of the above. Using the unique functionality afforded by this instrument, scientists can develop game changing ion-mobility acquisition strategies for structural analysis; varying storage and separation times to inves-tigate structural isomers, charge reduction and conformational transitions. G. Weibchen (Waters Eschborn)

Rapid Qualitative Analysis and Absolute Quantification of Plasma Proteins Using SONAR for Proteomic Clinical Research Studies Quantitative proteomics often incorporates the use of stable labeled isotopes in order to provide absolute quantification. Recent advancements have seen the introduction of peptide panels allowing the quantification of more than 500 proteins in plasma sample sets. However, this is technically challenging when attempting to acquire the data using more traditional MS acquisition modes such as multiple reaction monitoring, since the duty cycle of the instrument is compromised and therefore results in under sampling of the data. An alternative approach is to apply SONAR, a data independent analysis (DIA) methodology, allowing for high throughput while also ensuring high specificity and maintaining quantitative performance. SONAR is based on utilization of a fast scanning quadru-pole, enabling the technique to be compatible with fast chromatography, high throughput workflows. Clinical research using proteomics is one high throughput example which requires the analytical benefits provided by SONAR. Here, we present the applicability of SONAR for high throughput, absolute quantification of plasma proteins from human samples consisting of controls, chronic obstructive pulmonary disease, and asthma. S. Rexroth (Waters Eschborn)

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12:15–13:15 Lunch workshop l EvosepRoom H02

12:15–13:15 Lunch workshop l Thermo Fisher Scientific GmbHRoom H03 Unleash your OrbitrapTm: Innovations in hardware and workflows for deep proteome profiling in cooperation with PharmaFluidics

The use of µPAC™ columns for high sensitivity biomedical applicationsK. Mechtler (IMP-IMBA Vienna)

Expanding the limits of bottom up proteomics using pillar array columns and data-independent acquisition (DIA) L. Reiter (Biognosys)

13:20–14:20 news corner 2Room H02

Chair H. Meyer (Dortmund/DE)

13:30 Total solubilization of FFPE samples for high throughput clinical proteomicsNC6 J. Wilson (Farmingdale, NY/US), I. Ilieva (Philadelphia, PA/US) D. J. Pappin (Farmingdale, Cold Spring Harbor, NY/US) J. B. Wojcik (Philadelphia, PA/US)

13:42 Antithrombin analysis by mass spectrometry for clinical chemistry NC7 application: Quantitation and proteoform characterization R. Ruhaak, F. Romijn, M. Pieterse, J. Nouta, N. Smit, E. Dominguez-Vega Y. van der Burgt, M. Wuhrer, C. Cobbaert (Leiden/NL)

13:54 High-throughput protein complex profiling in mammalian tissues samplesNC8 C. Martelli, E. G. Williams (Zurich/CH), P. Xue (Zurich/CH; Beijing/CN) A. Fossati, F. Uliana, F. Frommelt, I. Bludau, T. Sajic, L. Gillet, B. C. Collins R. Aebersold (Zurich/CH)

14:06 Simplified human gut microbiota (SIHUMI) community cultivated in in vitro P122 bioreactors shows no changes towards varying nutrient flow rates S. Schäpe, J. L. Krause, H. Gunda, F. Jean, F. Katarina, R.-K. Ulrike F. Schattenberg, S. Müller, N. Jehmlich, M. von Bergen (Leipzig/DE)

14:18 Post-column solvent addition enhances microflow bottom-up proteomics NC10 in the low microgram range U. Distler, M. Łącki (Mainz/DE), M. Wanninger (Milford, MA/US) S. Tenzer (Mainz/DE)

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13:30–14:30 Symposium 7Room H03 Redox proteomicsChair T. Grune (Potsdam/DE)

13:30 Chlorination and oxidation of extracellular matrix proteins in IL18 cardiovascular disease A. Rogowska-Wrzesinska (Odense/DK)

13:55 Cause and effects of H2S-mediated redox unbalance in SP21 ALS proteome V. Greco, V. Corasolla Carregari, F. Marini, A. Spalloni, P. Longone L. Pieroni, A. Urbani (Rome/IT)

14:07 Sensitive and accurate comparative analysis of the metalloproteomics of SP22 anaerobically respiring microorganisms R. Budhraja, C. Ding, P. Walter, J. Mattusch, S. Wagner (Leipzig/DE) G. Sawers (Halle a. d. Saale/DE), L. Adrian (Leipzig/DE)

14:19 Mapping thiol oxidative modifications in primary haematopoietic stem cellsSP23 K. Pimkova, M. Jassinskaja, E. Johansson, J. Hansson (Lund/SE)

13:30–14:30 Symposium 8Room H04 Microbiome and microbial pathogensChair C. Carapito (Strasbourg/FR)

13:30 Elucidating the effector interactomes of bacterial pathogensIL19 P. van Damme, V. Jonckheere (Ghent/BE)

13:55 Inter-strain proteome variances of 27 planktonic and biofilm grown SP24 clinical Pseudomonas aeruginosa isolates J. Erdmann, J. G. Thöming, A. Pich (Hannover/DE), C. Lenz (Goettingen/DE) S. Häußler (Hannover, Braunschweig/DE)

14:07 Proteomics and immunoproteomics characterization of Staphylococcus P129 aureus strains causing infective endocarditis C. Hentschker, K. Surmann, S. Michalik, L. Busch, J. Iqbal, M. Gesell Salazar T. Meyer (Greifswald/DE), E. Ruotsalainen, V. P. Kontinen (Helsinki/FI) S. Fuchs (Wernigerode/DE), U. Mäder, B. M. Bröker, S. Holtfreter U. Völker (Greifswald/DE)

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14:19 An immunoproteomic approach identifies a panel of new candidate SP26 antigens for a diva workflow in brucellosis A. Soggiu (Milan/IT), V. Greco (Rome/IT), M. Tittarelli G. Garofolo (Teramo/IT), A. Spina (Catanzaro/IT), A. Janowicz, V. Paci F. Sacchini (Teramo/IT), C. Piras (Reading/GB), D. Britti (Catanzaro/IT) L. Bonizzi (Milan/IT), P. Roncada (Catanzaro/IT)

13:30–14:30 Symposium 9Room H01 new technologiesChair R. Kellner (Darmstadt/DE)

13:30 Cross-linking – Mass spectrometry for system-wide protein interaction IL20 studies A. Sinz (Halle a. d. Saale/DE)

13:55 HUNTER – A new method for sensitive identification of protein SP27 N-termini from microscale proteomes F. Demir (Juelich/DE), S. H. Weng (Vancouver/CA), S. Dirnberger D. Tuscher (Juelich/DE), E. Ergin, A. Uzozie, L. Nierves, J. Tsui P. Lange (Vancouver/CA), P. Huesgen (Cologne, Juelich/DE)

14:07 Novel aspects on chemical protein-RNA cross-linking coupled with SP28 mass spectrometry at amino acid resolutio A. Wulf, L. Welp, S. Vos, A. Stuetzer, S. Johansson, K. Sloan, R. Ficner M. Bohnsack, P. Cramer, H. Urlaub (Goettingen/DE)

14:19 Complex-centric proteome profiling in one day with SEC-SWATH-MS SP29 and short gradient analysis B. C. Collins (Zurich/CH), P. Xue (Zurich/CH; Beijing/CN), C. Martelli I. Bludau (Zurich/CH), G. Rosenberger (New York, NY/US) C. Nicod (Zurich/CH), M. Heusel (Lund/SE), A. Fossati, F. Uliana F. Frommelt (Zurich/CH), Y. Liu (West Haven, CT/US) R. Aebersold (Zurich/CH)

14:30–16:30 Poster session 31st level Posters Even poster numbers Please refer to page 39 for further information.

Find out more at www.msvision.comMSVision Dedicated to Mass Spectrometry

Find out more at www.msvision.com

The battle to discover specifi c protein biomarkers has been hampered by the complexity and dynamic range of proteins in plasma…

A challenge so well known it’s a cliché!

Biomotif, Karolinska Institutet & MS Vision have co-pioneered the pI-TRAP åcelus System for automated, MS-compatible, high speed,

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25

Comprehensive and reproducible technique

Ideal for studying extremely complex samples that could containthousands of analytes over a wide dynamic range

Collection of MS/MS data on everything detectable in the sample

Every peak is reliably and reproducibly quanti�ed to ensure complete detection of peptides and proteins.

Stop by SCIEX stand #9 at EUPA for more information.

AB Sciex is doing business as SCIEX. © 2019 AB Sciex. For research use only. Not for use in diagnostic procedures. The trademarks mentioned herein are the property of the AB Sciex Pte. Ltd. or their respective owners. AB SCIEX™ is being used under license. Related to RUO-MKT-03-8078-A

SWATH® Acquisition The Eternal Sample

Find out more at www.msvision.comMSVision Dedicated to Mass Spectrometry

Find out more at www.msvision.com

The battle to discover specifi c protein biomarkers has been hampered by the complexity and dynamic range of proteins in plasma…

A challenge so well known it’s a cliché!

Biomotif, Karolinska Institutet & MS Vision have co-pioneered the pI-TRAP åcelus System for automated, MS-compatible, high speed,

isoelectric, pre-fractionation of peptides & proteins

Divide your sample into 30 fractions in about one hour and conquer biomarker discovery !

26


14:30–15:30 Training course 4 I bruker Daltonik gmbHRoom H02 Benefits of multidimensional approaches for proteomics studies

The increased acquisition rate and sensitivity levels are not the sole benefits of PASEF for shotgun proteomics experiments, as the Trapped Ion Mobility Spectrometry (TIMS) allows adding a separation which is orthogonal to the chromatographic and TOF separation. In this workshop proteomics experts will cover the benefits of IMS and TIMS in particular for shotgun proteomics approaches on different biological samples.

Glycopeptide profiling in blood plasma for clinical applicationsH. Wessel, PhD (Radboud UMC/NL)

Proteomics of the human endometrium: defining the molecular mechanisms underlying endometriosisC. Pineau, PhD (Protim Core Facility/FR)

15:30–16:30 Training course 5 I ab scIeX germany gmbHRoom H01 A complete workflow in a cloud from DDA to differential expression analysis

The SCIEX cloud offers complete workflow from experiment manager, protein identification to differential expression analysis. Experiment manager helps to organize acquired datasets within multiomics study. ProteinPilotTM is used for discovery proteomics and OneOmicsTM is an app to visualize data and inter-pret it in biologically-relevant ways. OneOmicsTM a “Click & Easy” workflow that integrats next-generation proteomics (NGP) data with next-generation sequencing (NGS) data. Major benefit using the SCIEX cloud Platform is the relative ease of use with which the data could be analyzed compared with the previous workflow which required extensive user customization by writing scripts and formatting files. This enabled the researchers to focus on the biology rather than the complications associated with formatting datasets and transformation of files.

16:40–17:40 EuPA Young Investigator Prize sessionRoom H03

Chairs K. Mechtler (Vienna/AT), D. Resetar-Maslov (Rijeka/HR)

16:40 System-based identification of genetic and environmental regulators of SP25 virulence in mycobacterium tuberculosis A. Banaei-Esfahani (Zurich/CH), J. Feldmann, L. Folkman, S. Borrell A. Trauner, K. Borgwardt (Basel/CH), B. Collins (Zurich/CH) S. Gagneux (Basel/CH), R. Aebersold (Zurich/CH)

27


16:55 Large scale study of Human Mitochondrial Proteome: Untargeted P202 characterization of membrane proteins by high resolution mass spectrometry F. Marini, L. Pieroni, V. Corasolla Carregari, V. Greco, A. Urbani (Rome/IT)

17:10 Dynamics and nanoscale organization of the neuronal surfaceomeSP5 M. van Oostrum, B. Campbell, M. Müller, S. K. Tyagarajan B. Wollscheid (Zurich/CH)

17:25 From molecular classification of glioma to a novel therapeutic strategy P127 based on tumor associated macrophages M. Duhamel, F. Rodet, M. Wisztorski, M. Rose, L. Drelich, I. Fournier M. Salzet (Villeneuve d’Ascq/FR)

17:50–18:10 Young Proteomics Investigators Club (YPIC)-challenge presentationRoom H03

Chairs K. Mechtler (Vienna/AT), D. Resetar-Maslov (Rijeka/HR)

Team 1: IgNobels Team 2: Fun

16:40–17:40 Symposium 10Room H04 From data to information to knowledge to utilityChair M. Ralser (Berlin/DE)

16:40 XL-MS for all: New search engines for protein-protein, protein-DNA, and IL21 protein-RNA cross-linking analysis T. Sachsenberg, E. Netz, T. Dijkstra (Tuebingen/DE), A. Chernev, A. Stützer H. Urlaub (Goettingen/DE), O. Kohlbacher (Tuebingen/DE)

17:05 Prophane – Versatile annotation and interpretation of metaproteomic SP30 identifications H. Schiebenhöfer, K. Trappe (Berlin/DE), E. Schmid (Zurich/CH), T. Muth B. Y. Renard (Berlin/DE), K. Riedel (Greifswald/DE) S. Fuchs (Wernigerode/DE)

17:17 BIONDA: A free database for a fast information on published protein SP31 biomarkers and biomarker candidates A. Frericks-Zipper, M. Eisenacher, A. Mosig, L. Reinert M. Turewicz (Bochum/DE)

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17:29 Network propagation as a tool for improving functional interpretability SP32 and completeness of proteomics experiments G. Barel (Berlin/DE), N. Selevsek (Zurich/CH), H. Gmuender O. Clayton (Basel/CH), L. Kunz (Zurich/CH), L. Küpfer (Aachen/DE) A. Roth (Basel/CH), R. Schlapbach (Zurich/CH), R. Herwig (Berlin/DE)

16:40–17:40 Symposium 11Room H01 Human disease: cancer, neuro, cardio, rare, immunologyChair F. Amado (Aveiro/UA)

16:40 Phosphoproteomics for precision oncologyIL22 C. Jimenez, R. Beekhof, T. V. Pham, C. van Alphen, A. A. Henneman J. C. Knol, F. Rolfs, M. Labots, E. Henneberry, T. Y. S. Le Large, R. R. de Haas S. R. Piersma (Amsterdam/NL), V. Vurchio, A. Bertotti L. Trusolino (Torino/IT), H. M. W. Verheul (Amsterdam/NL)

17:05 Machine learning of 1566 prostate tissue proteomes uncovered protein SP33 signatures predicting prostate cancer survival Q. Zhong (Westmead/AU; Zurich/CH), T. Guo (Hangzhou/CN; Zurich/CH) T. Zhu (Hangzhou/CN), R. Shah (Westmead/AU), G. Chen (Hangzhou/CN) R. Poulos (Westmead/AU), J. Ljubicic (Zurich/CH) P. Hains, N. Lucas (Westmead/AU), Y. Zhu (Hangzhou/CN; Zurich/CH) R. Dorothea (Zurich/CH), R. Sun (Hangzhou/CN), H. Roest G. Rosenberger (Zurich/CH), J. Neumann, K. Charmpi (Cologne/DE) M. Manica, M. Buljan, W. Shao (Zurich/CH), G. Ruan (Hangzhou/CN) N. Rupp, D. Schirmacher, P. Patrick (Zurich/CH), R. Reddel P. Robinson (Westmead/AU), M. R. Martinez (Zurich/CH) A. Beyer (Cologne/DE), P. Wild (Zurich/CH; Frankfurt a. M./DE) R. Aebersold (Zurich/CH)

17:17 Mass spectrometry imaging in cancer diagnostics and cancer spheroids IL23 as drug testing model P. Hoffmann, M. Klingler-Hoffmann, M. R. Condina, M. Briggs, P. Mittal M. K. Oehler (Adelaide/AU)

19:30–23:59 Congress partyBiosphäre Please refer to page 70 for further information.

▐ High Performance Electrophoresis of Proteins ▐ Horizontal Large Gel Separation ▐ Compatible with 2D Western Blotting ▐ Advanced 2D Gel Image Analysis by Delta 2D

(classic, 2D DIGE, 2D Western Blot, 2D DIBE, and more approaches)

SERVA Electrophoresis GmbH – www.serva.de – [email protected] – Tel.: +49 6221 13840-0

HPE™ BlueHorizon™ From Protein Separation to 2D Analysis

We look forward

to see you!

Lunch Workshop Wednesday Mar 27 12:15 pm to 1:15 pm H02

„Electrophoresis Reloaded – Protein Separation live: Fast, fluorescent and furious“

Training Course Wednesday Mar 27 2:45 pm to 3:45 pm H02

„Mercator Gel Technology – Live on Stage“

Trypsin/Lys-C MixMass Spec Grade

• Increased Peptide and Protein Identifi cations

• Improved Reproducibility

• Tolerant to Trypsin-Inhibiting Contaminants

www.promega.com

29

▐ High Performance Electrophoresis of Proteins ▐ Horizontal Large Gel Separation ▐ Compatible with 2D Western Blotting ▐ Advanced 2D Gel Image Analysis by Delta 2D

(classic, 2D DIGE, 2D Western Blot, 2D DIBE, and more approaches)

SERVA Electrophoresis GmbH – www.serva.de – [email protected] – Tel.: +49 6221 13840-0

HPE™ BlueHorizon™ From Protein Separation to 2D Analysis

We look forward

to see you!

Lunch Workshop Wednesday Mar 27 12:15 pm to 1:15 pm H02

„Electrophoresis Reloaded – Protein Separation live: Fast, fluorescent and furious“

Training Course Wednesday Mar 27 2:45 pm to 3:45 pm H02

„Mercator Gel Technology – Live on Stage“

Trypsin/Lys-C MixMass Spec Grade

• Increased Peptide and Protein Identifi cations

• Improved Reproducibility

• Tolerant to Trypsin-Inhibiting Contaminants

www.promega.com

30

scIenTIfIc ProgramWednesday, march 27

08:30–10:00 Plenary session 5Room H03 Signaling networks, pathways and pathway activity (PTMs)Chair B. Küster (Munich/DE)

08:30 Dissecting oncogenic EGF receptor signaling in-vivo by quantitative IL24 interaction proteomics and phosphoproteomics J. Olsen (Copenhagen/DK)

09:00 Proteome dynamicsIL25 M. Selbach (Berlin/DE)

09:30 MCP lecture IL26 Drug effects on protein stability and proteostasis M. Bantscheff (Heidelberg/DE)


10:00–11:00 Training course 6 I bruker Daltonik gmbHRoom H02 Unlocking the power of a fourth dimension in mass spectrometry: Introduction of different post-processing software for PASEF data

Trapped ion mobility spectrometry (TIMS) provides an additional dimension of separation to LC-MS. Appropriate software that can extract the informa-tion contained in the 4D data space (m/z, retention time, TIMS and signal intensity) is required. In this workshop, experts will present their software solutions (MaxQuant, PEAKS studio, openSWATH) for the timsTOF Pro plat-form and how the fourth dimension can improve proteome coverage, quantification accuracy and dynamic range in proteomics analyses.

MaxQuant for timsTOF ProNikita Prianichnikov (Max Planck Institute of Biochemistry, J. Cox group/DE)

Integration of spectral library with sequence database and de novo sequencing for PASEF data W. Zhang (Bioinformatics Solution Inc./CA)

diaPASEF: Concepts and data analysis with openSWATHB. Collins (ETH Zurich/CH)

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10:30–12:00 Plenary session 6Room H03 Interactomics: Affi nity, proximity, and spati al proteomicsChair M. Ueffi ng (Tuebingen/DE)

10:30 Broad analysis of HLA class I pepti de epitopes profi led by mass IL27 spectrometry in mono-allelic cells improves neoanti gen binding predicti on S. Klaeger (Cambridge, MA/US), S. Sarkizova (Boston, MA/US) D. Keskin (Cambridge, Boston, MA/US), K. Clauser, H. Keshishian C. Harti gan (Cambridge, MA/US), N. Hacohen C. Wu (Cambridge, Boston, MA/US), S. Carr (Cambridge, MA/US)

11:00 The synapti c proteome: Abundance, proteoforms, lifeti mes, interacti onsIL28 H. Urlaub (Goetti ngen/DE)

11:30 Predicti on of redox-modifi ed cysteines using machine learning and IL29 stati sti cal methods on sequence and structural protein data B. Kessler (Oxford/GB), M. Kessler (Frankfurt a. M./DE)

12:00–13:30 Lunch break within the industrial exhibiti on

12:15–13:15 Lunch workshop l SERVA Electrophoresis GmbHRoom H02 in cooperati on with DECODON

The workshop presented by Serva Electrophoresisand Decodon allows an overview of the latest developments in High Performance Electrophoresis, Fluorescence Labeling, Detecti on (by SERVA) and Image Analysis (by Decodon Soft ware).„MERCATOR Gel technology on stage“: We will present main steps of protein electrophoresis and detecti on. SERVA will show how to run a large format horizontal HPE™ Mercator gel using BlueHorizon™ and to capture the image followed by fi nal (soft ware based) evaluati on (Delta 2D Decodon). Prof. Simone König, University Münster, the inventor of the technology, will present the theoreti cal background and potenti al applicati ons. In the separate SERVA lunch symposium (same day), SERVA introduces the new type of fi lm-based horizontal HPE gel suited for Western Blotti ng. Prac-ti cal aspects of handling will be addressed. SERVA also presents the new Mercator protein marker developed by Prof. König’s group. Decodon will show premium evaluati on of signals obtained aft er semi-dry protein transfer. „Get more out of your data“: Both training sessions aim to show latest tech-nical trends in rapid protein analysis. Benefi ts of the SERVA high performance gel electrophoresis, transfer and image analysis of Western Blots in Delta2D soft ware (by Decodon) are evident that enable scienti sts to getti ng superior, reliable resultsTake the chance to see how we can help you to make your daily lab life a bit easier. SERVA Electrophoresis and Decodon look forward presenti ng a smart workfl ow for your protein analysis. Join us and enjoy the live sessions.


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13:15–14:45 Career session – Hostet by the Young Proteomics Investigators Club (YPIC)Room H01

Chairs C. Moritz (Saint-Étienne/FR)

Employability has been one of the main goals of the Bologna process. However, many universities neglect training their students’ skills in career-planning, especially beyond academia. The Career Session can help to fillthis gap. Five well-known companies of the proteomics sector present theirfo-cuses and inform about requested competences for candidates. Currentjob vacancies – if available – will be presented. In addition, the chances, obsta-cles, and necessary skills of academic careers will be addressed in two of the talks.

Industry part13:15 Career advices from Thermo Fisher Scientific T. Börsig (Dreieich/DE)

13:28 Career advices from SERVA Electrophoresis B. Müller (Heidelberg/DE)

13:41 How to get a job in the biotech industry (TECAN Deutschland) C. Scherling (Crailsheim/DE)

13:54 Career perspectives in instrument manufacturer industry (MS Vision) M. Macht (Almere/NL)

14:07 Career advices from SCIEX P. Blankenstein (Darmstadt/DE)

Academic part14:20 Best Practice in EU grant applications J. Scherer, (St. Ingbert/DE)

14:33 How bumpy is the road to become a professor? – A professor reports tba

13:30–14:30 Symposium 12Room H03 Protein transport and subcellular localizationChair A. Pitt (Birmingham/UK)

13:30 Mitochondrial complexomicsIL30 U. Brandt (Nijmegen/NL; Cologne/DE)

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13:55 The human mitochondrial proteome defined in a cellular context SP34 through multifaceted quantitative proteomics M. Morgenstern, C. Peikert, C. Klemm, I. Suppanz, P. Lübbert (Freiburg/DE) N. Naumenko (Goettingen/DE), O. Alka, C. Hartmann, C. Schütze, S. Stiller F. Drepper, S. Oeljeklaus (Freiburg/DE), S. Dennerlein (Goettingen/DE) N. Pfanner, N. Wiedemann, B. Warscheid (Freiburg/DE)

14:07 A comprehensive protein proximity map for endogenous p53SP35 G. Vandemoortele, D. De Sutter, A. Moliere, K. Gevaert (Ghent/BE) F. Rousseau, J. Schymkowitz (Leuven/BE), S. Eyckerman (Ghent/BE)

14:19 Protein turnover within mitochondrial complexesSP36 J. Heidler, H. Giese, V. Strecker, I. Wittig (Frankfurt a. M./DE)

13:30–14:30 Symposium 13Room H04 Antibiotic resistance mechanisms – New antimicrobial compoundsChair U. Völker (Greifswald/DE)

13:30 Histidine phosphorylation: A new dimension in the phosphoproteomeIL31 C. Potel, S. Lemeer, N. Prust, M. H. Lin (Utrecht/NL)

13:55 Comparison of Proteomic Responses (CoPR) – An antibiotic mechanism SP37 of action discovery tool C. Senges, J. Stepanek, U. Ay, P. Prochnow, N. Janzing (Bochum/DE) T. Lukezic (Ljubljana/SI), N. Lorenz (Bochum/DE), H. Petkovic (Ljubljana/SI) N. Metzler-Nolte, J. Bandow (Bochum/DE)

14:07 A novel Re(I)-(CO)3 N-heterocyclic carbene complex antibiotic withSP39 multiple targets S. Schäkermann, D. Siegmund, P. Prochnow, L. Janzen, M. Engelhardt N. Metzler-Nolte, J. Bandow (Bochum/DE)

14:07 Culture free metaproteomics to evaluate antimicrobial resistance of bacterialSP38 consortia of raw milk C. Piras (Reading/GB; Milano/IT), A. Soggiu (Milano/IT), V. Greco (Rome/IT) A. Zecconi (Milano/IT), O. Hale, R. Cramer (Reading/GB) C. Gusmara (Milano/IT), D. Britti (Catanzaro/IT), L. Bonizzi (Milano/IT) A. Urbani (Roma/IT), P. Roncada (Catanzaro/IT)

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14:30–16:30 Poster session 41st level Posters Odd poster numbers Please refer to page 52 for further information.

14:45–15:45 Training course 7 I SERVA Electrophoresis GmbHRoom H02 Electrophoresis reloaded – Protein separation live: Fast, fluorescent and furious

The workshop presented by Serva Electrophoresis and Decodon allows an overview of the latest developments in High Performance Electrophoresis, Fluorescence Labeling, Detection (by SERVA) and Image Analysis (by Decodon Software).„MERCATOR Gel technology on stage“: We will present main steps of protein electrophoresis and detection. SERVA will show how to run a large format horizontal HPE™ Mercator gel using BlueHorizon™ and to capture the image followed by final (software based) evaluation (Delta 2D Decodon). Prof. Simone König, University Münster, the inventor of the technology, will present the theoretical background and potential applications. In the separate SERVA lunch symposium (same day), SERVA introduces the new type of film-based horizontal HPE gel suited for Western Blotting. Prac-tical aspects of handling will be addressed. SERVA also presents the new Mercator protein marker developed by Prof. König’s group. Decodon will show premium evaluation of signals obtained after semi-dry protein transfer. „Get more out of your data“: Both training sessions aim to show latest tech-nical trends in rapid protein analysis. Benefits of the SERVA high performance gel electrophoresis, transfer and image analysis of Western Blots in Delta2D software (by Decodon) are evident that enable scientists to getting superior, reliable results. Take the chance to see how we can help you to make your daily lab life a bit easier. SERVA Electrophoresis and Decodon look forward presenting a smart workflow for your protein analysis. Join us and enjoy the live sessions.

15:30–16:30 Training course 8 I Tecan Deutschland gmbHRoom H01 Semi-automated sample processing for proteomics

Meet our experts in MS sample prep. Our workshop speakers will present innovative workflows using positive pressure workstations and liquid handling platforms to prepare samples for proteomics and steroid analysis.

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16:40–17:40 Symposium 14Room H03 Host-pathogen interactionsChair J. Bandow (Bochum/DE)

16:40 Determination of host-pathogen quaternary protein structures in IL32 complex biological samples J. Malmström (Lund/SE)

17:05 Levels of human proteins in plasma associated with acute pediatric malariaSP40 P. Nilsson (Stockholm/SE)

17:17 Proteomic analysis of intracellular Bordetella pertussis: Moving towards SP41 a better understanding of bacterial persistence and pathogenesis K. Surmann (Greifswald/DE), Y. Lamberti, J. H. Cafiero H. Valdez (La Plata/AR), B. Vecerek, J. Holubova (Prague/CZ), F. Schmidt U. Völker (Greifswald/DE), M. E. Rodriguez (La Plata/AR)

17:29 Clostridium difficile toxins reorganize the proteome of target cellsSP42 T. Schweitzer, F. Stieglitz, J. Junemann, A. Wulf, J. Erdmann, R. Gerhard I. Just, A. Pich (Hannover/DE)

16:40–17:40 Symposium 15Room H04 Proteogenomics and clinical applicationsChair K. Mechtler (Vienna/AT)

16:40 Proteogenomics of colorectal cancer tumorsIL33 C. Borchers (Victoria/CA), B. Blank-Landeshammer A. Sickmann (Dortmund/DE), G. Batist (Montreal/CA)

17:05 Glycoproteogenomics: A frequent gene polymorphism affects the SP43 glycosylation pattern of human fetuin/α-2-HS-glycoprotein Y. Lin, A. Heck, V. Franc (Utrecht/NL)


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17:17 Advancing mass spectrometry-based large-cohort proteomics for SP44 precision medicine – An international cancer moonshot multiple site study Y. Xuan (Bremen/DE), N. W. Bateman (Bethesda, MD/US) S. Gallien (Paris/FR), Y. Zhou, M. Hu (Shanghai/CN) N. Parikh (Bethesda, MD/US), P. Navarro (Bremen/DE) B. Hood (Bethesda, MD/US), C. Lorenz, C. Loosse (Dortmund/DE) S. Goetze (Zurich/CH), R. B. Kitata (Taipei/TW), S. Piersma D. Chiasserini (Amsterdam/NL), H. Zhu (Shanghai/CN) G. Hou (Shenzhen/CN), M. Tahir, (Odense/DK), A. Macklin A. Khoo (Toronto/CA), X. Sun (Xián/CN), B. Crossett (Sydney/AU) A. Sickmann (Dortmund/DE), B. Wollscheid, (Zurich/CH) Y. Chen (Taipei/TW), C. Jimenez (Amsterdam/NL), H. Zhou (Shanghai/CN) S. Liu (Shenzhen/CN), M. R. Larsen (Odense/DK), T. Kislinger (Toronto/CA) Z. Chen (Xián/CN), B. L. Parker, S. J. Cordwell, (Sydney/AU) T. P. Conrads (Bethesda, MD, Annandale, VA/US)

17:29 Pepper – A proteogenomics pipeline for validated bacterial gene annotationSP45 S. Fuchs, A. Hasel (Wernigerode/DE), S. Engelmann (Braunschweig/DE) M. Wilkens (Braunschweig, Wernigerode/DE), E. Lehmann M. Kucklick (Braunschweig/DE)

16:40–17:40 Symposium 16Room H01 Technological InnovationsChair O. Trifonova (Moscow/RU)

16:40 Sensitive measurement of plasma proteins using parallel reaction SP47 monitoring and real-time targeting of 804 stable isotope standards with MaxQuant.live S. Müller (Schlieren/CH), F. Meier (Martinsried/DE), T. Gandhi O. Bernhardt (Schlieren/CH), M. Mann (Martinsried/DE) L. Reiter (Schlieren/CH)

16:52 Ultra-fast proteomics enabled by scanning SWATH in conjunction with SP48 high-flow chromatography and neural network-based processing software (DIA-NN) V. Demichev (Cambridge, London/GB), C. Messner S. Vernardis (London/GB), N. Bloomfield, G. Ivosev, F. Wasim S. Tate (Concord/CA), K. Lilley (Cambridge/GB) M. Ralser (London/GB; Berlin/DE)

17:04 Reproducibility, specificity and accuracy of relative quantification SP49 using spectral library-based data independent acquisition K. Marcus, S. Pacharra, K. Barkovits, K. Pfeiffer, S. Steinbach M. Eisenacher, J. Uszkoreit (Bochum/DE)

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17:16 Evaluation of targeted proteomics approaches on a TIMS-Q-TOFSP50 A. Lesur, J. Longworth (Luxembourg/LU), P.-O. Schmit (Wissembourg/FR) F. Bernardin, G. Dittmar (Luxembourg/LU)

17:28 Disussion

17:45–19:00 closing and award lectureRoom H03

Chairs K. Mechtler (Vienna/AT), U. Völker (Greifswald/DE)

Award Session 17:45 Promotion Prize of the DGPF17:53 Poster Prize DGPF18:00 YPIC Prize18:03 Poster Prize EuPA18:08 Industry Award EuPA18:13 Start Up Company Award18:18 Juan Pablo Albar Protein Pioneer Award

18:30 Closing Lecture IL34 A PTM code for membrane protein maturation J. Yates, S. Panlow, C. Bamberger (La Jolla, CA/US)

scIenTIfIc Program Thursday, march 2809:30–17:00 Educational workshopRoom H03 Tool-training for proteomics

In this one day course four proteomics software tools will be introduced. Attendees of the course will learn how to use the tools PIA, PeptideShaker, SearchGUI and BIONDA effectively for their daily work. This course is orga-nized as a common teaching event of the EuBIC initiative and the de.NBI/ELIXIR Germany service center BioInfra.Prot.

09:00 Registration09:20 Setting up the virtual machine09:45 Introduction to EuBIC, de.NBI, BioInfra.Prot & ELIXIR10:00 KNIME11:00 PIA12:30 Lunch (at own expense)13:30 PeptideShaker / SearchGUI15:00 Coffee break15:30 BIONDA16:30 Discussion17:00 End

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PosTer sessIon 1 I PosTer sessIon 3

Please note that all abstracts are printed as handed-in.

P2 Sperm proteome analysis of the hooded oysterK. Thanormjit (Saensook, Mueang, ChonBuri/TH), C. Srisomsap, D. ChokchaichamnankiM. R. J. Svasti (Lak Si, Bangkok/TH), S. Kingtong (Saensook, Mueang, ChonBuri/TH)

P4 Induction and molecular characterization of Systemic Acquired Resistance (SAR) for the control of Fusarium Wilt in AbacaJ. Mati-Om (Baybay Leyte/PH), R. Gapasin, E. Tulin, R. Piamonte (Baybay/PH)L. J. Oyong (Bontoc, Southern Leyte/PH)

P6 The interplay of proteins in ALS and prion diseases E. Teferedegn, C. Un, D. Tesfaye (Izmir/TR)

P8 Metabolomic fatty acids analysis in high-fat diet induced obese rats: The possible protective effect of antinflammatory interventions.R. Ghazala, A. Elmedenay, A. Meleis, P. Mohey El din, H. Samir (Alexandria/EG)

P10 Effects of foliar application of 24-epibrassinolide on growth and induction of resistance of maize plants to Helminthosporiosis on the fieldK. T. William Norbert, N. D. Jules Patrice, A. Heu, A. S. Thierry, A. ZachéeT. T. Paul Martial (Yaoundé/CM)

P12 Urine – The next generation early disease biomarker sourceY. Gao (Beijing/CN)

P14 Ultrafast and universal proteomic sample preparation – From intact tissue to LC-MS injection in 15 minJ. Döllinger, A. Schneider, P. Lasch (Berlin/DE)

P16 Development of a fully automated pipeline for phosphoproteome profilingS. Stoychev, N. Previn, I. Govender, G. Isak, J. jordaan (Pretoria/ZA)

P18 A Multi-pathway enrichment approach for quantitative profiling of cellular signalingS. Aravamudhan (Leiden/NL)

P20 A LC-MS/MS method to detect reactive oxygen species reveals the unstable nature of hydroethidine-based probesY. Xiao, D. Meierhofer (Berlin/DE)

P22 Reduction of inflammatory effects associated with O-GlcNAcylation by silibinin in NASH miceO.-S. Kwon, S. J. Lee, D. E. Lee (Daegu/KR)

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P24 Proteomic profiling of F508 del-CFTR expressing cells to identify new pharmacological targets for Cystic FibrosisC. Braccia (Legnano, Genova/IT), V. Tomati, E. Caci, N. Pedemonte, A. Armirotti (Genova/IT)

P26 The wool proteome in three distinct ovine breeds from PortugalJ. Plowman (Lisboa/PT; Lincoln/NZ), A. Campos (Porto/PT), S. R. Silva (Vila Real/PT)A. Thomas, C. van Koten, S. Clerens (Lincoln/NZ), A. de Almeida (Lisboa/PT)

P28 Dietary spirulina and lysozyme in piglet feeding: A study on the hepatic proteomeC. Martins (Lisboa/PT), J. Kuleš (Zagreb/HR), D. Ribeiro (Lisboa/PT), A. Horvatić, N. Guillemin V. Mrljak (Zagreb/HR), J. A. M. Prates, J. B. Freire (Lisboa/PT)P. D. Eckersall (Zagreb/HR; Glasgow/GB), A. de Almeida (Lisboa/PT)

P30 Mitochondrial phosphoproteome analysis during neurogenesis reveals new regulators of energy metabolismL. Hoell, S. Amin, R. Malty, M. T. Moutaoufik, Z. Minic, M. Babu (Regina/CA)

P32 A decrease of fructose-1,6-bisphosphatase in papillary renal cell carcinoma indicates a fundamental role in tumorigenesisA. Alahmad, A. Rabien, K. Jung, D. Meierhofer (Berlin/DE)

P34 Multi-attribute monitoring (MAM) to identify differences in Trastuzumab from 2 manufacturersT. Shibata (Tokyo/JP), S. Heidelberger (Warrington/GB), J. Dojahn (Darmstadt/DE)

P36 Fast microflow chromatography for accelerating protein identification experimentsD. Merkel, D. Waidelich, J. Dojahn (Darmstadt/DE), N. Morrice (Warrington/GB)C. Hunter (Redwood, CA/US), Z. Demianova (Darmstadt/DE)

P38 Proteoformer 2.0: Further developments in the ribosome profiling-assisted proteogenomic hunt for new proteoformsS. Verbruggen, E. Ndah, W. Van Criekinge (Ghent/BE), S. Gessulat, B. KüsterM. Wilhelm (Munich/DE), P. Van Damme, G. Menschaert (Ghent/BE)

P40 Renal oncocytomas rewire glutathione metabolismG. Kürschner, K. Jung, D. Meierhofer (Berlin/DE)

P42 Probing for the scaffolding functions of the Cellular Master Switch AktM. Lindemann, N. Uhlenbrock, M. Schmidt, J. Weisner, I. Landel, M. MüllerD. Rauh (Dortmund/DE)

P44 Studying HapX iron sensing in Aspergillus fumigatus by label-free quantificatioM. Scheven, T. Krüger (Jena/DE), M. Misslinger (Innsbruck/AT), P. HortschanskyO. Kniemeyer (Jena/DE), H. Haas (Innsbruck/AT), A. A. Brakhage (Jena/DE)

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P46 Towards deciphering proteoforms: Chromosome-centric lessonsS. Naryzhny (Moscow, Gatchina, Leningrad/RU), E. Zorina, A. KopylovV. Zgoda (Moscow/RU), N. Ronzhina, O. Kleyst (Gatchina, Leningrad/RU)A. Archakov (Moscow/RU)

P48 Mass spectrometry-based phosphoproteomics to uncover PD-1/PD-L1 signalingS. Petzoldt (Freising, Munich, Heidelberg/DE), J. Zecha (Heidelberg, Munich, Freising/DE)E. Hui (San Diego, CA/US), B. Küster (Freising, Munich, Heidelberg/DE)

P50 xiView: A common platform for the downstream analysis of Cross-Linking/Mass Spectrometry dataM. Graham, C. Combe, L. Kolbowski, J. Rappsilber (Edinburgh/GB)

P52 Data-dependent (DDA) and data-independent (DIA) label free quantification (LFQ) of the CSF proteome of non-human primatesA. Schmidt (Munich/DE), H. Ito (Osaka/JP), D. Moechars, B. Hrupka (Beerse/BE), S. MüllerS. Lichtenthaler (Munich/DE)

P54 Phosphoproteome study on Staphylococcus aureus to identify phosphoproteins involved in virulenceN. Prust, C. M. Potel, N. van Sorge, S. Lemeer (Utrecht/NL)

P56 Integrative structural proteomics analysis of the 20S Proteasome ComplexR. Viner, D. Horn (San Jose, CA/US), E. Damoc (Bremen/DE), A. Konijnenberg (Eindhoven/NL)S. Memmi (Breda/NL)

P58 The mitochondrial interactome in baker’s yeast: A snapshot taken by cross-linking mass spectrometryA. Linden, R. Pflanz, I. Parfentev, B. Homberg, M. Deckers, P. RehlingH. Urlaub (Goettingen/DE)

P60 BioInfra.Prot: Bioinformatics services for proteomicsT. Sunny, M. Turewicz, K. Schork, G. Mayer, M. Eisenacher (Bochum/DE)

P62 Identification of candidate biomarkers of HPV-associated sinonasal squamous cell carcinoma using LC-MS/MST. Ekalaksananan, T. Nukpook, C. Pientong (Khon Kaen/TH), S. Roytrakul (Pathum thani/TH) W. Teeramatwanich, N. Patarapadungkit, P. Vatanasapt (Khon Kaen/TH)

P64 Hands-off: Fully automated and TMT-compatible sample preparation in less than4 hours on the PreOn platform employing the iST-NHS technology F. Hosp, D. Jansen, N. Kulak (Martinsried/DE)

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P66 High throughput signaling pathway analysis using multiplex-immunoprecipitation and fast LC-PRMS. Gallien (Villebon sur Yvette/FR), A. Gajadhar (San Jose, CA/US), B. Patel (Rockford, IL/US)T. Arrey (Bremen/DE), D. Sarracino, S. Trusiak (Cambridge, MA/US)C. Dauly (Villebon sur Yvette/FR),Y. Xuan (Bremen/DE), E. I. Chen (Cambridge, MA/US)

P68 Comparative phosphorylation map of human Dishevelled 3 (hDVL3)K. Hanáková, O. Bernatík, P. Ovesná, M. Kravec, M. Micka, M. Rádsetoulal, D. PotesilL. Cajánek, Z. Zdráhal, V. Bryja (Brno/CZ)

P70 Decision path for choosing a normalization method for a quantitative proteomics data setK. Schork, M. Turewicz, M. Kohl, M. Eisenacher (Bochum/DE)

P74 Workflow for the processing of bottom-up label-free proteomic experimentsK. Gömöryová, P. Paclíková, T. Radaszkiewicz, Z. Zdráhal, V. Bryja, D. Potěšil (Brno/CZ)

P76 High Sensitivity Phosphoproteomics using PASEF on a TIMS-QTOF mass spectrometerH. Koch (Bremen/DE), K. Desch (Frankfurt a. M./DE), S. Koch, T. KosinskiP. Brechlin (Bremen/DE), E. Schuman, J. Langer (Frankfurt a. M./DE)

P78 PASEF on a TIMS-QTOF instrument for reproducible, sensitive and high-throughput shotgun proteomicsS. Koch, H. Koch, T. Kosinski, M. Lubeck, R. Hebeler (Bremen/DE), A. Brunner, F. MeierM. Mann (Martinsried, Munich/DE)

P80 The response of the saliva proteome in sheep infected with liver fluke (Fasciola hepatica) D. Meltzer (Glasgow/GB), P. Skuce, G. Mitchell (Edinburgh/GB), R. Zadoks (Glasgow/GB)A. Horvatic, J. Kules (Zagreb/HR), R. Burchmore (Glasgow/GB), V. MrljakD. Eckersall (Zagreb/HR)

P82 Analysis of the tobacco pollen sequestromeZ. Zdrahal, D. Potesil (Brno/CZ), S. Hafidh, K. Müller, J. Fila, C. Michailidis, A. HerrmannovaJ. Fecikova (Prague/CZ), T. Ischebeck (Goettingen/DE), L. S. Valášek, D. Honys (Prague/CZ)

P84 Learning ubiquitous factors from millions of mass spectra – Deploying a general spectrum processorT. Altenburg, T. Muth, B. Y. Renard (Berlin/DE)

P86 Proteomic analysis of brain-specific proteins in minipig Huntington disease modelI. Poliakh (Libechov, Prague/CZ), J. Tylečková (Libechov/CZ)J. Červenka (Libechov, Prague/CZ), J. Novák, P. Vodička (Libechov/CZ)

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P88 Identification of possible new salivary biomarkers of stress in sheep using high-resolution quantitative proteomic studyA. Horvatić (Zagreb/HR), D. Escribano (Barcelona/ES), M. D. Contreras-Aguilar (Murcia/ES)N. Guillemin (Zagreb/HR), J. Cerón, M. Lopez-Arjona, M. L. Hevia, X. Manteca (Murcia/ES)V. Mrljak (Zagreb/HR), P. D. Eckersall (Zagreb/HR; Glasgow/GB)

P90 Post-transcriptional regulation of phytohormone signal transduction in Arabidopsis immunityM. Abukhalaf, D. Thieme, C. Proksch, J. Ziegler, W. Hoehenwarter (Halle a. d. Saale/DE)

P92 Proteomic analysis of seminal plasma exosomes: Comparison of 3 detergents in filter-aided sample preparation for LC-MS/MSH. Kupcova Skalnikova (Libechov/CZ), J. Cervenka (Libechov, Prague/CZ)K. Turnovcova (Prague/CZ), J. Novak, B. Bohuslavova, P. Vodicka (Libechov/CZ)

P94 Identification of Sus Scrofa immune organs proteins for creation immuno regulatorA. Akhremko, E. Vasilevskaya (Moscow/RU)

P96 Development of a targeted proteomics method for investigation of cyclooxygenase-2 expressionN. M. Hartung (Wuppertal/DE), S. Immenschuh (Hannover/DE)N. H. Schebb (Wuppertal/DE)

P98 Proteomics profiling of nasal polyps and HPV-associated nasal polypsC. Pientong, T. Nukpook, T. Ekalaksananan (Khon Kaen/TH), S. Roytrakul (Pathum Thani/TH)W. Teeramatwanich, N. Patarapadungkit, P. Vatanasapt (Khon Kaen/TH)

P100 Development of quantitative targeted proteomics for the diagnosis of Lyme borreliosisP. Cantero, N. Boulanger, B. Jaulhac, Y. Hansmann, C. Lenormand, L. Zilliox (Strasbourg/FR) P. Kieffer (Mulhouse/FR), L. Ehret-Sabatier (Strasbourg/FR)

P102 Effect of TMT label on peptide fragmentation and subsequent handling by different search enginesT. Mathieson, M. Kösters, C. Fufezan, M. Bantscheff (Heidelberg/DE)

P104 In search of the meaning in proteoformic profilesO. Kiseleva, A. Lisitsa, E. Ponomarenko, E. Poverennaya (Moscow/RU)

P106 Determining the phosphorylation dynamics in human spliceosomeK.-T. Pan, I. Silbern, M. N. Leelaram, O. Dybkov, R. Lührmann, H. Urlaub (Goettingen/DE)

P108 Proteomic coverage and analysis reproducibility in mAB producing CHO cellsL. Brachtvogel, T. Noll, R. Hoffrogge (Bielefeld/DE)

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P110 Relative quantification of TMT-labeled, cross-linked proteins using XlinkX node in Proteome DiscovererP. Pankert, R. Viner (Dreieich/DE)

P112 Finding in vivo drug targets in Danio rerio by thermal proteome profilingN. Leijten, P. Bakker, J. den Hertog, S. Lemeer (Utrecht/NL)

P114 An optimized enrichment strategy for improved mass spec analysis of chemically cross-linked peptidesM. Janda (Dreieich/DE), R. Viner (San Jose, CA/US), L. Foster, E. Raja, C. EtienneR. Bomgarden (Rockford, IL/US)

P118 Protein markers and risk of type 2 diabetes and prediabetes: A targeted proteomics approach in the KORA F4/FF4 studyC. von Törne, C. Huth, F. Schederecker, T. de las Heras Gala (Neuherberg/DE)C. Herder (Duesseldorf/DE), F. Kronenberg (Innsbruck/AT), C. Meisinger (Augsburg/DE) W. Rathmann (Duesseldorf/DE), W. König (Munich/DE), M. Waldenberger (Neuherberg/DE) M. Roden (Duesseldorf/DE), A. Peters, B. Thorand, S. M. Hauck (Neuherberg/DE)

P120 Detecting relevant molecular interactions in patient-data-derived multi-omics biomolecular networks for drug predictionK. Baum (Strassen/LU; Berlin/DE), J. C. Rajapakse (Singapore/SG), F. Azuaje (Strassen/LU)

P124 Data-independent acquisition analysis of the mitochondrial proteome to identify quantitative, disease-specific signaturesF. Klose, C. J. Mageean, S. Wende, K. Boldt, J. Fizgerald, M. UeffingC. J. Gloeckner (Tuebingen/DE)

P126 Dissecting the regulatory components of epithelial transport by interactome analysis of all human Claudin family proteinsL. Suarez-Artiles, E. Ramberger, T. Breiderhoff, D. Müller, P. Mertins (Berlin/DE)G. Dittmar (Luxembourg/LU)

P128 Using redox proteomic methods to investigate the hypoxic stress response of Aspergillus fumigatusL. Ivanova (Jena/DE), E. Shekhova (Aberdeen/GB), T. Krüger (Jena/DE)L. Leichert (Bochum/DE), O. Kniemeyer, A. A. Brakhage (Jena/DE)

P130 xMAPr – High-dynamic-range (HDR) quantification of antigen specific antibody bindingT. Meyer, F. Schmidt, U. Völker, S. Michalik (Greifswald/DE)

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P132 Characterizing the repertoire of targeted autoantigens of autoimmune neuropathiesC. Moritz (Saint-Priest-en-Jarez/FR), O. Stoevesandt (Cambridge/FR), E. Reynaud-FederspielD. Simonnet (Saint-Priest-en-Jarez/FR), Y. Tholance (Saint-Priest-en-Jarez, Saint-Étienne/FR)C. Rosier (Saint-Étienne, Saint-Priest-en-Jarez/FR)K. Ferraud (Saint-Priest-en-Jarez, Saint-Étienne/FR), M. Taussig (Cambridge, MA/US)J.-P. Camdessanché, J.-C. Antoine (Saint-Priest-en-Jarez, Saint-Étienne/FR)

P134 Targeted quantitative analysis of Serum Adipokines and Apolipoproteins by LC-MS/MSL. Krieg, A. Schaffert, M. Kern, M. Blüher (Leipzig/DE), K. Schubert (Leipzig/DE; Oxford/GB)M. von Bergen (Leipzig/DE)

P136 Multi-omics to develop nanomaterial grouping strategies for risk assessmentI. Kratochvil (Leipzig/DE), A. Bannuscher (Berlin/DE), B. Hellack (Duisburg/DE)A. Haase (Berlin/DE), K. Schubert (Leipzig/DE; Oxford/GB), M. von Bergen (Leipzig/DE)

P138 Prezygotic mate selection in the self-compatible Nicotiana attenuata results from coordinated variation in self-incompatibility genesH. Guo, R. Halitschke, N. Wielsch, K. Gase, A. Svatos, I. Baldwin (Jena/DE)

P142 Depletion of high molecular mass proteins for the identification of small proteins and short open reading frame encoded peptides in cellular proteomesL. Cassidy, P. Kaulich, A. Tholey (Kiel/DE)

P146 Optimised Desorption Electrospray Ionisation Mass Spectrometry Imaging (DESI-MSI) method for the analysis of proteins/peptides directly from tissue sectionsG. Weibchen (Eschborn/DE), M. Towers (Wilmslow/GB), E. Claude (Eschborn/DE)T. Beaty (Eschborn/DE; Milford, MA/US)

P148 Characteristics of different multidimensional data independent acquisition techniques on a single MS platformM. Kipping (Eschborn/DE), C. Hughes, K. Richardson, P. H (Manchester/GB)

P150 Integrated multi-omics and systems biology analysis of the targeted treatment of obesity within mouse modelsG. Weibchen (Eschborn/DE), M. van der Lienden (Leiden/NL), G. KramerN. Dekker (Amsterdam/NL), L. Gethings, J. Vissers (Wilmslow/GB)C. Argmann (New York, NY/US), J. Aerts (Leiden/NL), M. van Eijk (Leiden, Amsterdam/NL)

P152 Targeted proteomics-driven computational modeling of the mouse macrophage toll-like Receptor Signaling PathwayN. P. Manes, J. M. Calzola, P. R. Kaplan, M. Meier-Schellersheim, I. FraserA. Nita-Lazar (Bethesda, MD/US)

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P154 The end of Kozak Dogma: The Ghost ProteomeM. Salzet (Villeneuve d’Ascq/FR)

P156 Protein complexes as a regulatory tool in MollicutesO. Bukato, O. Pobeguts, I. Butenko, M. Levites, V. Ladygina, D. Evsyutina, G. FisunovV. Govorun (Moscow/RU)

P158 Method optimization for acetylome analysis using cell line MEC-1H. Kuchaříková, G. Lochmanová, K. Mikulášek, D. Potěšil, Z. Zdráhal (Brno/CZ)

P160 A comparative study of the internalization of two recombinant α-galactosidase A, employed in the treatment of Fabry-Anderson diseaseI. Iacobucci, C. Vollaro, F. Cozzolino, V. Citro, M. V. Cubellis, P. Pucci, M. Monti (Naples/IT)

P162 Proteome analysis of Streptococcus suis under stress conditions and in host-pathogen interactionD. Dittmar (Greifswald/DE), C. Weiße (Leipzig/DE), P. Hildebrandt, U. Mäder, M. Gesell Salazar, S. Michalik (Greifswald/DE), C. Baums (Leipzig/DE), U. Völker (Greifswald/DE)

P164 Proteomic insight reveals cold adaptive and anti-freeze potential in Iodobacter sp PC H194, a psychrotroph isolated from Indian Trans-HimalayaP. Kashyap, V. Kumar, S. Kumar, D. Singh (Palampur, Himachal Pradesh/IN)

P168 The proteome of schizophrenia brainsG. Reis-de-Oliveira, M. Fioramonte, G. S. Zucolli, V. de Almeida (Campinas/BR), A. SchmittP. Falkai (Munich/DE), D. Martins-de-Souza (Campinas/BR)

P170 Tissue sampling for proteomics and metabolomics with the picosecond Infrared laserH. Schlüter (Hamburg/DE)

P172 Picky: A user friendly online SRM & PRM method designerH. Zauber, M. Kirchner, M. Selbach (Berlin/DE)

P174 Moonlighting proteins of Bacillus cereus – Potential virulence factors, identified by SurfaceomicsM. Kranzler (Vienna/AT), N. Jeßberger (Oberschleissheim/DE), T. Buchacher (Vienna/AT)E. Märtlbauer (Oberschleissheim/DE), M. Ehling-Schulz (Vienna/AT)

P176 Peptide-based quality control in urine: (Quantitative) impact of correct sampling on peptidomeT. Barth, A. Petrera, C. Gar, A. Lechner, S. M. Hauck, C. von Törne (Munich/DE)

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P178 Integrated FTIR imaging and proteomics for identification of a histochemical biomarker candidate in bladder cancerK. Witzke, F. Großerueschkamp, H. Jütte, M. Horn (Bochum/DE), F. RoghmannN. von Landenberg (Herne/DE), T. Bracht, A. Kallenbach-Thieltges, H. Käfferlein, T. BrüningK. Schork, M. Eisenacher, K. Marcus (Bochum/DE), J. Noldus (Herne/DE), A. TannapfelB. Sitek, K. Gerwert (Bochum/DE)

P180 Studying PI3K-AKT-mTOR-S6K and RAF-MEK-ERK-RSK signaling in exercising skeletal muscle cells through global quantitative phosphoproteomicsA. L. Fricke, L. Reimann, C. D. Peikert, G. Radziwill, B. Warscheid (Freiburg/DE)

P182 In vivo cell surface protein biotinylation to characterize the differential protein expression between the neonatal and adult cardiac vasculatureT. Kocijan, R. ElMergawy (Trieste/IT), O. Shevchuk (Dortmund/DE), S. VodretM. Myers (Trieste/IT), A. Sickman (Dortmund/DE), S. Zacchigna (Trieste/IT)

P186 The influence of the honeybee venom peptides on the human erythrocytes membrane proteomeA. Swiatly-Blaszkiewicz, L. Mrówczyńska, G. Rosiński, J. Matysiak (Poznan/PL)

P188 Maps interaction protein complexesT. Koshlan, K. Kulikov (Saint-Petersburg/RU)

P190 Tracking Host Cell Proteins while biopharmaceutical manufacturing: Advanced methodologies to improve product qualityS. Wohlrab (Penzberg/DE)

P192 Proteomic phenotyping of mouse models for myelin-related diseasesO. Jahn, S. Siems, D. Hesse (Goettingen/DE), S. Tenzer (Mainz/DE)H. Werner (Goettingen/DE)

P194 Data processing approach for the construction and evaluation of an organism’s UNIQUOME with comparative analysis for the Human, Rat and Mouse UniquomesE. Kontopodis, V. Pierros, A. Anagnostopoulos, D. Stravopodis, I. Papassideri, C. VorgiasG. Tsangaris (Athens/GR)

P196 Unraveling the metagenomic potential: New strand for processing next generation sequencing dataL. T. Kishi, E. M. Lopes, C. C. Fernandes, E. S. G. Peppe, E. G. de Macedo LemosM. V. F. Lemos (Jaboticabal/BR)

P198 Mass spectrometry-based analysis of the royal jelly proteomeE. Matuszewska, P. Dereziński, M. Witkowska, A. Światły-Błaszkiewicz, Z. KokotJ. Matysiak (Poznan/PL)

47


P200 Impact of venom immunotherapy on protein/peptide profiles in patients allergic to Hymenoptera venomE. Matuszewska (Poznan/PL), J. Matysiak (Kalisz/PL), P. DerezińskiA. Światły-Błaszkiewicz (Poznan/PL), S. Kosiński, E. Smorawska-Sabanty (Lodz/PL)Z. Kokot (Poznan/PL), M. Kowalski (Lodz/PL), J. Matysiak (Poznan/PL)

P202 Large scale study of Human Mitochondrial Proteome: Untargeted characterization of membrane proteins by high resolution mass spectrometryF. Marini, L. Pieroni, V. Corasolla Carregari, V. Greco, A. Urbani (Rome/IT)

P204 The articular cartilage proteome is dependent on zone, age and disease stateA. Smagul, D. Simpson, S. Tew, M. Peffers (Liverpool/GB)

P206 Data-independent acquisition spiked with QconCAT quantification standards (DISQ): An alternative to targeted acquisition?F. C. Sigloch (Bad Abbach/DE), F. Metzger, U. Ohmayer (Munich/DE)A. Bhangu-Uhlmann (Bad Abbach/DE), S. Hauck (Munich/DE)

P208 Functional characterization of SPANX-A/D family in human spermatozoa and melanomaI. Urizar-Arenaza, N. Osinalde, A. Benedicto, A. Perez, I. Muñoa-Hoyos, J. A. RodriguezA. Asumendi, M. D. Boyano, N. Subiran (Leioa/ES), I. Kratchmarova (Odense/DK)

P210 Comprehensive proteome analysis of Xanthomonas campestris pv. campestris B100V. Ortseifen, J. Voß, L. Brachtvogel, R. Hoffrogge, T. Noll, K. Niehaus (Bielefeld/DE)

P212 Influence of antibiotic therapy on lung proteome adaptation in murine pneumoniaS. Blankenburg (Greifswald/DE), S. Berger (Berlin/DE), S. Michalik, M. Gesell SalazarC. Hentschker (Greifswald/DE), C. Goekeri, N. Suttorp (Berlin/DE), J. Vera (Erlangen/DE)M. Witzenrath, G. Nouailles (Berlin/DE), U. Völker (Greifswald/DE)

P214 Unveiling the outer membrane vesicle proteome of the fish pathogen A. salmonicidaT. Kroniger, A. Trautwein-Schult (Greifswald/DE), B. Köllner (Greifswald-Insel Riems/DE)D. Becher (Greifswald/DE)

P216 The neighborhood of LRRK2 – Revisiting the LRRK2 interactome by proximity labelingM. Eckert, F. von Zweydorf, C. J. Gloeckner (Tuebingen/DE)

P218 Biomarker discovery in Parkinson’s disease by LC-MS/MS analysis of cerebrospinal fluid and urineS. Virreira Winter, O. Karayel, J. Bader, M. Steger (Martinsried/DE)P. Geyer (Copenhagen/DK; Martinsried/DE), N. Skotte (Copenhagen/DK)M. Mann (Martinsried/DE; Copenhagen/DK)

48


P220 Characterization of bacterial isolates from parsley phyllosphere by MALDI-TOF mass spectrometryM. Heinze, A.-C. Scherwinski, S. Ruppel, K. Witzel (Grossbeeren/DE)

P222 The impact of simulated microgravity in pancreatic adenocarcinoma cellsM. Manfredi, M. A. Masini, V. Vanella, E. Bixio, B. Valentina, E. Robotti (Alessandria/IT)F. Masetto, M. Donadelli (Verona/IT), E. Marengo (Alessandria/IT)

P224 Integrated serum proteomics and metabolomics for putative biomarker discovery in inflammatory bowel diseaseM. Manfredi (Verona/IT), E. Conte, E. Barberis, E. Robotti (Alessandria/IT), D. CecconiJ. Brandi (Verona/IT), V. Caneparo, M. Gariglio, M. De Andrea (Novara/IT)E. Marengo (Alessandria/IT)

P226 Dissection of YAP1 proteoforms and interactionsF. Uliana, A. Fossati, R. Ciuffa, M. Mehnert, F. Frommelt, R. AebersoldM. Gstaiger (Zurich/CH)

P228 Proteomics of Diatoms: Discovery of Polyamine modifications in biosilica-associated proteinsA. Milentyev, C. Heintze, N. Poulsen, N. Kröger, A. Shevchenko (Dresden/DE)

P230 Quantification and elucidation of the metabolic channelling of Xanthomonas campestris pv. campestris B100 proteins involved in substitution of the polysaccharide xanthan by pyruvic acid or acetyl groupsJ. Voß, V. Ortseifen, K. Niehaus (Bielefeld/DE)

P232 Exploring the mitochondrial degradome by quantitative proteomics in a cellular model of Parkinson’s diseaseM. Lualdi (Busto Arsizio/IT), M. Ronci (Chieti/IT), F. Corno, E. S. Turilli, M. SponchiadoT. Alberio, M. Fasano (Busto Arsizio/IT)

P234 Comparison of MS-cleavable crosslinkersJ. Singh, D. Winter (Bonn/DE)

P236 Structural modeling of a bacterial laccaseB. Bonfá Buzzo, G. Cabral Fernandes, E. Soares Gomes-Pepe, É. Mendes LopesE. G. de Macedo Lemos (Jaboticabal/BR)

P238 Reproducible and hands-off sample preparation workflows for mass mpectrometry with focused ultrasonicationM. Werner, K. Amerault, V. Process, L. Jansen (Woburn, MA/US), N. Autret (Brighton/GB)V. Meyyappan (Woburn, MA/US), D. Pappin, J. Wilson (New York, NY/US)

49


P240 Absolute quantification of the lysosomal proteome by Multiple Reaction Monitoring and QconCAT protein standardsP. R. Mosen (Bonn/DE), R. Sakson (Heidelberg/DE), E. Kaade (Bonn/DE)T. Ruppert (Heidelberg/DE), V. Gieselmann, D. Winter (Bonn/DE)

P242 Simulation of human gastrointestinal metabolism of soy allergens in an in-vitro model and proteome analysis by LC-HRMS/MSJ. Bräcker, J. Brockmeyer (Stuttgart/DE)

P244 In silico characterization of a prospected and annotated bacterial hemicellulase in a genome from a microbial consortiumP. A. Maldaner Pereira, E. Soares Gomes-Pepe, E. G. de Macedo LemosM. V. F. Lemos (Jaboticabal/BR)

P246 Screening of non-proteinogenic molecules as tool for quality control in mass-spectrometry-based proteomicsF. Metzger, M. Linnerbauer, J. Merl-Pham, S. Hauck (Munich/DE)

P248 Can you trust your model? A showcase study of validation in 13C metabolic flux analysisN. Sundqvist (Linkoeping/SE)

P250 Systems biology of MHC class I antigen presentation studied in human cancer cell linesJ. Hahlbrock (Mainz/DE), P. Navarro (Bremen/DE), S. Boegel, U. Sahin, H. SchildS. Tenzer (Mainz/DE)

P252 Mass spectrometry-based identification of body fluids for forensic purposesK. Barkovits, J. Stepien, S. Roocke (Bochum/DE), S. Kuhlmann (Duesseldorf/DE)A. Dorn (Munich/DE), K. Marcus (Bochum/DE)

P254 AmyloGram: Prediction of protein amyloidogenicityJ. Chilimoniuk (Wrocław/PL), M. Burdukiewicz (Warsaw/PL), P. Sobczyk (Wrocław/PL)S. Rödiger (Senftenberg/DE), M. Gąsior-Głogowska, M. KotulskaP. Mackiewicz (Wrocław/PL)

P256 The first phosphoproteome-wide mechanistic model of insulin signalingW. Lövfors, E. Nyman, G. Cedersund (Linkoeping/SE)

P258 Proteomics in translational researchM. Mann (Martinsried/DE)

P260 Occupational smoke exposure may modify the expression of respiratory tract proteomaS. Neves, S. Pacheco, F. Vaz, V. M. Torres (Lisbon/PT), P. James (Cologne/DE), T. SimoesD. Penque (Lisbon/PT)

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P262 Search and investigation of potential peptide agents of interaction between human organism and its microbiomeG. Arapidi, A. S. Urban, I. O. Butenko, V. O. Shender, M. S. Osetrova, G. A. NosO. M. Ivanova, T. M. Saveleva, A. N. Mitin, N. I. Sharova, M. F. Nikonova, A. I. MartinovE. N. Ilina, V. T. Ivanov, V. M. Govorun (Moscow/RU)

P264 Monitoring cellular networks – In vivo XL-MS in B cellsJ. Schwarz, A. Linden, J. Corso, M. Engelke, J. Wienands, H. Urlaub (Göttingen/DE)

P266 Muscle proteins turn-over during exercise and ageingF. Marchiano, N. Luis (Marseille/FR)

Editor-in-Chief:Andrew PittAston University, Birmingham, UK

Editor-in-Chief:Juan J. CalveteResearch Professor of the Spanish Research Council (CSIC), Professor of PEDECIBA (Uruguay), Head of the Structural and Functional Venomics Laboratory, Instituto de Biomedicina de Valencia, Spain

Since 2008, Journal of Proteomics has been publishing fundamental and clinical research in diverse � elds of proteomics research, aimed at protein scientists and analytical chemists.

Research areas include: • Human, animal, microbial and plant proteomics • Non-conventional and non-model organism proteomics • Biomarker discovery • Protein analytics or bioinformatics • Tissue imaging by mass spectrometry • Metabolomics • Genomics • Systems biology • Toxicogenomics • Pharmacoproteomics

EuPA Open Proteomics is an open access, online only scienti� c journal focused on all aspects of proteomics, from basic to translational research. The journal publishes articles that are built on a solid experimental design and explored with robust data analysis techniques.

EuPA Open Proteomics welcomes: • Benchmark datasets • Protocols • Reviews and opinions • Original research articles • Heralded datasets.

EuPA Open ProteomicsJournal of Proteomics

Elsevier publishes these and more proteomics journals Visit the Elsevier booth for more information about scienti� c publishing or to pick up your free journal copy.*

elsevier.com/locate/euprotelsevier.com/locate/jprot

2017

1.80*

3.7722017 Journal Impact Factor

*2018 Journal Citation Reports®

(Clarivate Analytics, 2019)

2017

3.85

Published by Elsevier on behalf ofAn o� cial journal of

*Subject to availability

For the full aims and scope, or to submit your paper, visit:

51

Editor-in-Chief:Andrew PittAston University, Birmingham, UK

Editor-in-Chief:Juan J. CalveteResearch Professor of the Spanish Research Council (CSIC), Professor of PEDECIBA (Uruguay), Head of the Structural and Functional Venomics Laboratory, Instituto de Biomedicina de Valencia, Spain

Since 2008, Journal of Proteomics has been publishing fundamental and clinical research in diverse � elds of proteomics research, aimed at protein scientists and analytical chemists.

Research areas include: • Human, animal, microbial and plant proteomics • Non-conventional and non-model organism proteomics • Biomarker discovery • Protein analytics or bioinformatics • Tissue imaging by mass spectrometry • Metabolomics • Genomics • Systems biology • Toxicogenomics • Pharmacoproteomics

EuPA Open Proteomics is an open access, online only scienti� c journal focused on all aspects of proteomics, from basic to translational research. The journal publishes articles that are built on a solid experimental design and explored with robust data analysis techniques.

EuPA Open Proteomics welcomes: • Benchmark datasets • Protocols • Reviews and opinions • Original research articles • Heralded datasets.

EuPA Open ProteomicsJournal of Proteomics

Elsevier publishes these and more proteomics journals Visit the Elsevier booth for more information about scienti� c publishing or to pick up your free journal copy.*

elsevier.com/locate/euprotelsevier.com/locate/jprot

2017

1.80*

3.7722017 Journal Impact Factor

*2018 Journal Citation Reports®

(Clarivate Analytics, 2019)

2017

3.85

Published by Elsevier on behalf ofAn o� cial journal of

*Subject to availability

For the full aims and scope, or to submit your paper, visit:

52


Please note that all abstracts are printed as handed-in.

P1 Membrane protein analysis of oyster spermS. Kingtong (Chonburi/TH), K. Kellner, B. Bernay, P. Sourdaine, C. Heude Berthelin (Caen/FR)

P3 Effect of DDT on proteome profile of oyster tissue and potential biomarker of DDT exposureS. Chueycham (Chonburi/TH), C. Srisomsap, D. Chokchaichamnankit, J. Svasti (Bangkok/TH)S. Kingtong (Chonburi/TH)

P5 Biological control of the date palm tree borers, Oryctes spp (Coleoptera: Scarabaidae: Dynastinae)M. Khalaf, H. Alrubeai (Baghdad/IQ)

P7 Pre-analytical variation in protein biomarker researchL. Pollack (Uppsala/SE)

P9 Discoidin, CUB and LCCL domain-containing protein 2 (DCBLD2) is a novel biomarker of myxobrosarcoma invasion identified by global protein expression profilingK. Kikuta (Tochigi, Tokyo/JP), A. Yoshida, M. Nakamura, M. Matsumoto, A. KawaiT. Kondo (Tokyo/JP)

P11 Proteomics, “proteome-mix” or proteoformics – unraveling by next generation settingsH. Rhode, P. Muckowa, R. Büchler, S. Wendler, B. Tautkus, M. Vogel, T. MooreJ. Grosskreutz, A. Klemm (Jena/DE)

P13 Perceptions about the nanoparticles interaction with wheat proteomeF. Yasmeen, S. Komatsu (Swabi/PK)

P15 MALDI-TOF plasma protein profiling for the detection of early breast cancer and correlation with clinicopathhological featuresP. Moez, D. Younan, N. Abouseada, A. T. Awad, A. Ghoneim (Alexandria/EG)

P19 Maximize the output of routine proteome analyses by using a 50 cm long microfabricated nano LC columnR. van Ling, C. Mitterer, G. van Raemdonck (Zwijnaarde, Ghent/BE)

P21 Uncharacterized mitochondrial protein and respirasome assemblyM. T. Moutaoufik, R. Malty, L. Hoell, M. Babu (Regina/CA)

P23 Identification and characterization of tumor-derived exosomes through proteomics approachesR. Rana, J. Kumari (Delhi/IN)

53


P25 Proteome changes of extracellular vesicles isolated from the plasma of myelodysplastic syndrome patientsK. Pecankova, P. Majek, T. Riedel, E. Jäger, J. Cermak, J. E. Dyr (Prague/CZ)

P27 The effects of dietary spirulina and lysozyme supplementation on the piglet’s intestinal mucosa proteomeC. Martins (Lisboa/PT), J. Kuleš (Zagreb/HR), D. Ribeiro (Lisboa/PT), A. Horvatić, N. GuilleminV. Mrljak (Zagreb/HR), J. A. M. Prates, J. Freire (Lisboa/PT)P. D. Eckersall (Zagreb/HR; Glasgow/GB), A. de Almeida (Lisboa/PT)

P29 Elucidation of wound healing responses after cold atmospheric plasma treatmentJ.-W. Lackmann, S. Bekeschus, K. Masur, K. Wende (Greifswald/DE)

P31 Simplifying the use of ion libraries during data processing of data independent acquisition proteomics dataD. Waidelich (Darmstadt/DE), N. Morris (Warrington/GB), J. DojahnD. Merkel (Darmstadt/DE), M. Huebsch, A. Lau (Redwood, CA/US), C. Hunter (Concord/CA)

P33 Establishment of farmed fish welfare biomarkers – A multi-omics approachC. Raposo de Magalhães, D. Schrama, M. Cerqueira, A. P. Farinha (Gambelas, Faro/PT)A. Kuehn, D. Revets (Esch-sur-Alzette/LU), S. BoonanuntanasarnC. Nakharuthai (Nakhon Ratchasima/TH), P. M. Rodrigues (Gambelas, Faro/PT)

P35 Identification and absolute quantification of individual Host Cell Proteins by SWATH® Aquisition LC-MSJ. Dojahn (Darmstadt/DE), R. R. Lund, S. B. Nielsen, J. Crawford, J. BunkenborgA. Rasmussen, E. Mørtz, T. Kofoed (Odense/DK)

P37 Plasma protein targets for early detection and monitoring of sinusoidal obstruction syndromeP. Majek, K. Pecankova, J. Vydra, V. Valkova, A. Vitek, J. Dyr (Praha/CZ)

P39 Characterization of the plasma proteome in normal and overconditioned dairy cows during the transition from late pregnancy to early lactationM. Hosseini Ghaffari (Bonn/DE), K. Schuh (Bonn, Bingen a. R./DE), J. Kuleš, N. GuilleminA. Horvatić, V. Mrljak (Zagreb/HR), P. D. Eckersall (Zagreb/HR; Glasgow/GB)G. Dusel (Bingen a. R./DE), C. Koch (Muenchweiler a. d. A./DE), H. Sadri (Tabriz/IR)H. Sauerwein (Bonn/DE)

P41 Quantitative elution of biotinylated peptides and proteins from streptavidin complexesU. Warnken, M. Schnölzer (Heidelberg/DE)

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P43 Proteomic and metabolomic stratification of Equine OsteoarthritisJ. Anderson, P. Clegg, M. Phelan, E. Caamaño-Gutiérrez, L. Rubio-Martinez, C. RiggsM. Peffers (Liverpool/GB)

P45 The queen conch (Lobatus gigas) proteome: A valuable tool for biological studies in marine gastropodsD. Domínguez-Pérez (Porto/PT), J. Lippolis (Ames, IA/US), M. Dennis, B. MillerK. Tiley (Basseterre/KN), V. Vasconcelos (Porto/PT), A. de Almeida (Lisboa/PT)A. Campos (Porto/PT)

P47 Proteogenomics of adenosine-to-inosine RNA editing in brain proteomes of fruit fly, mouse and humanS. Moshkovskii, K. Kuznetsova, A. Kliuchnikova, A. Goncharov, L. Levitsky, D. KarpovM. Gorshkov (Moscow/RU)

P49 Purple: A fully automated software for strategic selection of peptides in viral diagnostics using MS-based targeted proteomicsJ. Lechner, F. Hartkopf, P. Hiort, J. Döllinger, B. Renard, T. Muth (Berlin/DE)

P51 MaxQuant software for trapped ion mobility enhanced shotgun proteomicsN. Prianichnikov, C. Wichmann (Martinsried/DE), S. Beck, H. Koch, M. Lubeck (Bremen/DE)J. Cox (Martinsried/DE)

P53 The canine faecal proteome in chronic intestinal diseaseE. O’Reilly, A. Ridyard (Glasgow/GB), A. Horvatic, J. Kules, N. Guillemin, V. MrljakD. Eckersall (Zagreb/HR)

P55 rawDiag – Accessing orbitrap data on the fast laneT. Kockmann, C. Panse, C. Trachsel (Zurich/CH)

P57 viQC: Visual and intuitive quality control for bottom-up proteomicsE. Solovyeva (Dolgoprudny, Moscow/RU), A. Lobas (Moscow/RU), A. Surin (Pushchino/RU)V. Gorshkov (Odense/DK), A. Kopylov, M. Gorshkov (Moscow/RU)

P59 Protein expression profiles and biomarkers identification for HPV-associated sinonasal inverted papilloma by LC-MS/MST. Nukpook, C. Pientong, T. Ekalaksananan (Khon Kaen/TH), S. Roytrakul (Pathum thani/TH) W. Teeramatwanich, N. Patarapadungkit, P. Vatanasapt (Khon Kaen/TH)

P61 A mass spectrometry based method to validate the LiMAx breath testR. Gajowski, T. Wünsch, M. Stockmann, S. Bulik, H.-G. Holzhütter, D. Meierhofer (Berlin/DE)

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P63 Development of a multiple reaction monitoring-mass spectrometry assay for the quantification of myeloid-derived growth factor in plasma samples from healthy individuals and patients with coronary artery diseaseF. Polten, M. Reboll, K. Bethmann, L. Bodo (Hannover/DE)C. Widera (Oldenburg, Hannover/DE), E. Giannitsis (Heidelberg/DE), J. Tillmanns, T. KempfJ. Bauersachs, A. Pich, K. C. Wollert (Hannover/DE)

P65 The canine plasma proteome in neoplastic and inflammatory diseasesR. Furioso Ferreira (Zagreb/HR), R. Mischke (Hannover/DE), J. Kuleš, A. HorvatićN. Guillemin, V. Mrljak (Zagreb/HR), D. Eckersall (Zagreb/HR; Glasgow/GB)

P67 Differentiation between wild and farmed Sea Bream using high resolution mass spectrometry and two-dimensional electrophoresisC. Guglielmetti, S. Sciuto, G. Esposito, S. Brusadore (Turin/IT)M. Manfredi (Alessandria Novara/IT), E. Marengo (Alessandria/IT), P. L. AcutisM. Mazza (Turin/IT)

P69 Accelerating DIA studies to extend workflow utility, using fast microflow LC gradientsN. Morrice, C. Hunter, Z. Demianova (Warrington/GB)

P71 MS/MS spectrum streaming for near real-time protein identificationK. Schallert, R. Zoun (Magdeburg/DE), S. Brehmer (Bremen/DE), D. Benndorf, G. SaakeU. Reichl (Magdeburg/DE)

P73 Identification of ISG15-target proteins during Coxsackievirus B3 infectionC. Bredow, M. Kespohl (Berlin/DE), F. Thery, F. Impens (Ghent/BE)K.-P. Knobeloch (Freiburg/DE), A. Beling (Berlin/DE)

P75 Skeletal muscle proteomics analysis reveals changes associated with dietary amino acid supplementation in unweaned calvesK. Yu (Bellaterra/ES), J. Kuleš, N. Guillemin, A. Horvatić (Zagreb/HR)M. Terré (Caldes de Montbui/ES), V. Mrljak (Zagreb/HR)P. D. Eckersall (Zagreb/HR; Glasgow/GB), A. Bassols (Bellaterra/ES)

P77 Development of the human proteome peptide catalog – A comprehensive repository of reference peptides for the human proteomeK. Schnatbaum (Berlin/DE), D. P. Zolg, M. Wilhelm (Freising/DE), T. Knaute, J. ZerweckH. Wenschuh (Berlin/DE), B. Kuster (Freising/DE), U. Reimer (Berlin/DE)

P79 A new approach for end-user driven quantification of SIL peptides for targeted proteomicsK. Schnatbaum, L. Hornberger, J. Zerweck, T. Knaute, H. Wenschuh, U. Reimer (Berlin/DE)

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P81 Analysis of host cell proteins in antibody preparation using PASEFS. Pengelley, S. Koch (Bremen/DE), G. Tremintin (San Jose, CA/US), D. SuckauJ. Boosfeld (Bremen/DE), M. Greig (San Jose, CA/US)

P83 Hepatic, muscular and adipose tissues proteomic characterization in the Muskox (ovibos moschatus): Differences between males and femalesD. M. Ribeiro (Lisboa/PT; Esch a. d. Alzette/LU), S. PlanchonC. Leclercq (Esch a. d. Alzette /LU), K. Raundrup (Nuuk/GL)S. P. Alves, R. J. B. Bessa (Lisboa/PT), J. Renaut (Esch a. d. Alzette/LU)A. M. Almeida (Lisboa/PT)

P85 Maximizing throughput, robustness and analytical depth for shotgun proteomicsS. Koch, T. Kosinski, C. Meier-Credo, S. Kuehne, C. Gebhardt, H. Koch (Bremen/DE)

P87 MetaProteomics Pipeline (MPP): Integrating metaproteomics analysis tools into a modularised workflowK. Trappe, H. Schiebenhöfer, T. Muth, B. Y. Renard (Berlin/DE), S. Fuchs (Wernigerode/DE)

P89 Proteomic study of histone modifications in plantsG. Lochmanova, D. Ledvinová, K. Mikulášek, H. Kuchaříková, S. Brabencová, M. FojtováZ. Zdráhal (Brno/CZ)

P91 Mass spectrometry-based investigations of cold atmospheric plasma-induced post-translational modifications (PTM’s) in peptidesS. Wenske, J.-W. Lackmann, T. von Woedtke, K.-D. Weltmann, K. Wende (Greifswald/DE)

P93 Biochemical and structural insights into the transcriptional regulators MAT1-1-1 and MAT-1-2-1 from the penicillin-producing fungus Penicillium chrysogenumB. Ramšak, U. Kück (Bochum/DE)

P95 Evaluation of peptide quantification – Susceptibility for technical bias from batch effects and batch compensationJ. Willforss, F. Levander (Lund/SE)

P97 Assessment of sample preparation techniques for bottom-up proteomicsK. Mikulasek, H. Konecna, D. Potesil, Z. Zdrahal (Brno/CZ)

P99 An improved, high-throughput Parallel Reaction Monitoring (PRM) analysis pipeline using both MS1 and MS2 dataT. Gandhi, O. Bernhardt, S. Müller, I. Lienert, L. Reiter (Schlieren/CH)

P101 Rapid identification of intact staphylococcal bacteriophages using MALDI-TOF mass spectrometryO. Sedo, D. Stverakova, M. Benesik, Z. Zdrahal, J. Doskar, R. Pantucek (Brno/CZ)

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P103 Proteomics unravels metabolic changes in skeletal muscles of hibernating brown bearsB. Chazarin, A. Ziemianin (Strasbourg, Paris/FR), S. Chanon (Lyon/FR)M. Plumel (Strasbourg/FR), A. Evans (Koppang/NO), J. M. Arnemo (Koppang/NO; Ås/SE)G. Gauquelin-Koch (Paris/FR), K. Storey (Carleton/CA), C. Simon (Paris/FR)S. Blanc (Strasbourg/FR), E. Lefai (Saint-Genès Champanelle/FR), F. Bertile (Strasbourg/FR)

P105 Prediction and identification of small proteins in Staphylococcus aureus using a proteogenomics approachM. Kucklick, E. Lehmann, M. Wilkens, A. Mustafayeva, T. Ludwig, M. Diwo, J. WissingL. Jänsch (Braunschweig/DE), S. Fuchs (Wernigerode/DE), S. Engelmann (Braunschweig/DE)

P107 Rapid proteome profiling of heart tissue by PCT-SWATH mass spectrometry informs cardiac research and diagnosticsC. Lenz, L. Neuenroth, S. Brandenburg, S. Lehnart, H. Urlaub (Goettingen/DE)

P109 Peptide Ion Mapping Program: A fast and reliable peak classification in mass spectrometry spectra using machine learningF. Hartkopf, T. Muth, B. Y. Renard (Berlin/DE)

P111 Development of a quality control standard for Tandem Mass Tags (TMT) workflowsS. Radau, A. M. Robitaille (Dreieich/DE)

P113 Integration of proteomics and metabolomics data: A use case from the bacterial worldM. Ferro, J. Novion-Ducassou, F. Combes (Grenoble/FR), A. Perret (Evry/FR)L. Cottret (Castanet-Tolosan/FR), D. Vallenet (Evry/FR), E. Thevenot (Gif, Yvette/FR)

P115 Discovering regulatory networks of cholesterol homeostasis by identifying oxysterol target proteins using thermal proteome profilingC. Rossetti, L. Laraia (Kongeny Lyngby/DK)

P117 Application of the novel diaPASEF method for short gradientsS. Kaspar-Schoenefeld, M. Lubeck, H. Koch, S. Koch, O. Raether (Bremen/DE), F. MeierA.-D. Brunner (Martinsried/DE), A. Ha, M. Frank (Toronto/CA), B. CollinsR. Aebersold (Zurich/CH), H. Roest (Toronto/CA)M. Mann (Martinsried/DE; Copenhagen/DK)

P119 Comprehensive Proteome Mapping of a human cancer cell line using LC-FAIMS Pro-MS/MSS. Ndiaye (Villebon sur Yvette/FR), R. Huguet, S. Prasad, J. Silveira, G. McAlister, P. M. RemešD. J. Bailey, Q. Song, M. W. Belford, E. R. Wouters, J.-J. Dunyach, V. ZabrouskovS. E. Abbatiello (San Jose, CA/US)

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P121 de.NBI – The German Network for Bioinformatics InfrastructureD. Kleefisch, K. Schork, T. Sunny, M. Turewicz, M. Eisenacher (Bochum/DE), R. AlkhateebT. Dammann-Kalinowski, D. Jording, A. Tauch, D. Wibberg, M. WittchenA. Pühler (Bielefeld/DE)

P123 A metabolic labeling strategy for relative protein quantification in Clostridioides difficilA. Trautwein-Schult, S. Maaß, K. Plate, A. Otto, D. Becher (Greifswald/DE)

P125 An outside perspective – surface protein and secretome analysis on spores of allergenic fungiA. Pschibul, M. Blango, T. Krüger, T. Zheng, J. Li, G. Panagiotou, O. KniemeyerA. A. Brakhage (Jena/DE)

P127 From molecular classification of glioma to a novel therapeutic strategy based on tumor associated macrophagesM. Duhamel, F. Rodet, M. Wisztorski, M. Rose, L. Drelich, I. FournierM. Salzet (Villeneuve d’Ascq/FR)

P131 Cell-surface Proteomics: New perspective for identifications of toxic DinoflagellatesK.-y. Chan, H. K. Kong, K. W. H. Kwok, C.-l. Lo (Hunghom, Kowloon, Hong Kong/CN)

P133 Poor transcript-protein correlation in the brain: Negatively correlating gene products reveal neuronal polarity as a potential causeC. Moritz (Saint-Priest-en-Jarez/FR; Kaiserslautern/DE), T. Mühlhaus (Kaiserslautern/DE)S. Tenzer (Mainz/DE), T. Schulenborg (Kaiserslautern, Langen/DE)E. Friauf (Kaiserslautern/DE)

P135 Stability and variability of protein structures at phosphorylation: Bioinformatics analysis of spatial features in PDB dataB. Sobolev, D. Karasev, A. Veselovsky, V. Zgoda, A. Archakov (Moscow/RU)

P137 Application container and KNIME metanodes for reproducible processing of omics dataD. Potesil, K. Gomoryova, M. Bednarik, M. Cupak, V. Bryja, Z. Zdrahal (Brno/CZ)

P139 Transcriptomic and proteomic response of the filamentous fungus Aspergillus nidulans to low temperature stressB. Hanf, T. Krüger, D. Mattern, M. Stroe (Jena/DE), B. Bunk, J. Overmann (Braunschweig/DE)A. Brakhage, O. Kniemeyer (Jena/DE)

P141 Proteome alterations during progression of Duchenne muscular dystrophy: Insights from a clinical relevant transgenic pig modelT. Fröhlich, H. Tamiyakul, E. Kemter, F. Flenkenthaler, K. Otte, N. Klymiuk, E. WolfG. Arnold (Munich/DE)

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P143 A targeted proteomics assay for the detection of potential bioterror virusesM. Grossegesse, J. Doellinger, A. Nitsche (Berlin/DE)

P145 Global plasma protein profiling of a dilated cardiomyopathy patient cohortV. M. Dhople, E. Hammer, M. Dörr, K. Lehnert, S. Ameling, K. Weitmann, S. FelixU. Völker (Greifswald/DE)

P147 Rapid analysis of NCI60 Cell Line Panel Metabolic and lipid profiles with an automatic well plate reader using laser-assisted REIMSG. Weibchen (Eschborn/DE), R. Schaffer (Budapest/HU), J. Balog (Budapest/HU; London/GB)D. Simon, N. Kucsma, A. Lovrics, G. Szakacs (Budapest/HU), S. Pringle (Wilmslow/GB)Z. Takats (London/GB)

P149 Rapid qualitative and absolute quantification of plasma based proteins using a novel scanning quadrupole DIA acquisition methodM. Kipping (Eschborn/DE), C. Hughes, S. Lennon, L. Gethings, D. Heywood (Manchester/GB)F. Marty, S. Müller, L. Reiter (Schlieren/CH), R. Plumb (Milford, MA/US)

P151 Towards routine intact level quantification of monoclonal antibody in plasma by HRMSS. Rexroth (Eschborn/DE), Y. W. Alelyanus, M. D. Wrona, H. ShionC. Dunning (Milford, MA/US), J. Kirk (Manchester/GB)

P153 Effects of methionine supplementation on the performance of European seabass using a shotgun proteomics approachA. P. Farinha Resende, M. Cerqueira, D. Schrama, C. Raposo de Magalhães (Faro/PT)R. Colen, S. Engrola (Gambelas, Faro/PT), P. M. Leal Rodrigues (Faro/PT)

P155 Charge reversal peptide derivatization combined with SCX chromatography and 18O labeling improves proteome wide identification of protein C-terminiP. Kaleja, A. O. Helbig, A. Tholey (Kiel/DE)

P157 Quantitative analysis of protein, mRNA and microRNA response to hypoxia in primary hippocampal neurons C. L. Schiffmann, A. Zieseniss, C. Lenz, S. Burkhardt, G. Jain, H. Urbanke, D. M. KatschinskiH. Urlaub, A. Fischer (Goettingen/DE)

P161 Chip-based sensing of stress-induced membrane release of unprocessed Cell Surface Proteins in vitro and in serumG. Müller (Oberschleissheim/DE), A. Herling (Frankfurt a. M./DE)K. Stemmer (Oberschleissheim/DE), A. Lechner (Munich/DE)M. Tschöp (Oberschleissheim/DE)

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P163 The role of electrostatic interaction in the formation of protein complexes, for example proteins p53, p63 and p73T. Koshlan, K. Kulikov (Saint-Petersburg/RU)

P165 Polymorphism of Ghrelin genes among four populations as tools for improvement of Nigerian indigenous chickensI. Emmanuel-Udeozor, H. Foleng-Ndofor, S. Ugwu (Nsukka/NG)

P167 Identification and quantification of Host Cell Proteins in biopharmaceuticals using a novel data-independent acquisition mode and MS/MS spectral library searchA. Corbin (Saint Quentin en Ivelines/FR)

P169 Protease-based approaches for improved characterization of biotherapeutic proteinsM. Rosenblatt, S. Saveliev, C. Hosfield, C. End, M. Urh (Madison, WI/US)

P173 Evaluation of PQ500, an Assay Panel Covering more than 500 proteins, in plasma samples of subjects with NSCLC and pancreatic cancer using high-throughput MRM mass spectrometryS. Müller, N. Dupuis, T. Treiber, C. Escher, L. Reiter (Schlieren/CH)

P175 Severity based alterations of the proteome pattern in patients with community-acquired pneumonia (CAP)M. Gesell Salazar (Greifswald/DE), T. Kacprowski (Greifswald, Freising-Weihenstephan/DE)S. Michalik (Greifswald/DE), P. Ahnert (Leipzig/DE), M. Kiehntopf (Jena/DE)M. Löffler (Leipzig/DE), N. Suttorp (Berlin/DE), U. Völker (Greifswald/DE)

P177 Development of Parallel Reaction Monitoring (PRM) applied to HER2-positive breast cancerL. Camoin, M. Guerin, Y. Toiron, E. Baudelet, M. Pophillat, S. GranjeaudP. Fourquet (Marseille/Fr), W. Jacot (Montpellier/Fr), C. Tarpin, R. Sabatier, E. AgavnianP. Finetti, J. Adelaide, D. Birnbaum, C. Ginestier, E. Charafe-Jauffret, P. ViensF. Bertucci, J.-P. Borg (Marseille/Fr)

P179 Low pH sample preparation method for accurate analysis of PTMs in biotherapeutic proteinsS. Saveliev (Madison, WI/US), C. End (Mannheim/DE), M. UrhM. Rosenblatt (Madison, WI/US)

P181 Novel Workflow for MS-based IgG quantification in preclinical pharmacokinetic studiesM. Rosenblatt (Madison, WI/US), C. End (Mannheim/DE), N. Nath, M. Urh (Madison, WI/US)

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P183 Reducing the risk of misdiagnosis of indirect ELISA by normalizing serum-specific background noise: The example of detecting anti-FGFR3 autoantibodiesC. Moritz (Saint-Priest-en-Jarez/FR), Y. Tholance (Saint-Priest-en-Jarez, Saint-Étienne/FR)F. Lassablière (Saint-Priest-en-Jarez/FR), J.-P. CamdessanchéJ.-C. Antoine (Saint-Priest-en-Jarez, Saint-Étienne/FR)

P185 Recent updates to Pyteomics and Python proteomics softwareL. Levitsky, M. Ivanov (Moscow/RU), J. Klein (Boston, MA/US), M. Gorshkov (Moscow/RU)

P187 Qualitative analysis of the dissociation constant (Kd) in the interaction of peptides with full-length proteinsT. Koshlan, K. Kulikov (Saint-Petersburg/RU)

P189 Searching for serum biomarkers of ovarian cancer using iTRAQ-based proteomicsA. Swiatly-Blaszkiewicz, A. Horała, J. Matysiak, J. Hajduk, E. Nowak-MarkwitzZ. Kokot (Poznan/PL)

P191 VAMS vs DBS: Which technology is better for blood metabolites profiling?O. Trifonova, D. Maslov, E. Balashova, P. Lokhov, A. Lisitsa, A. Archakov (Moscow/RU)

P193 Systematic assessment of nuclear complexes dynamics upon mammalian dosage compensationA. Fossati, F. Uliana, F. Frommelt, M. Gstaiger, R. Aebersold, A. Wutz (Zurich/CH)

P195 Feta cheese proteins: The identity of Greece’s national treasureT. Anagnostopoulos, G. Tsangaris (Athens/GR)

P199 LC-MS/MS proteomic analysis of secreted protein fractions from Corynebacterium glutamicum and its PPIase FkpA deletion mutantA. Wirtz, M. Klees, M. Bott, T. Polen (Juelich/DE)

P201 Molecular detection of Leishmania infantum DNA and host blood meal identification in Phlebotomus in a hypoendemic focus of human leishmaniasis in northern AlgeriaB. Kahina (Boumerdes/DZ), T. Djamel (Boumerdes/DZ; Marseille/FR)L. Ismail (Blida/DZ; Marseille/FR), B.-L. Amina (Tipaza/DZ), B. Idir (Marseille/FR; Alger/DZ)P. Philippe (Marseille/FR)

P203 The membrane proteome and phosphoproteome of MycN-activated neuroblastoma cellsA. Tsakaneli (London/GB), V. Corasolla Carregari, F. Marini, L. Pieroni, V. GrecoA. Urbani (Rome/IT), A. Sala (London/GB), G. Palmisano (Rome/IT; São Paulo/BR)

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P205 Impact of PEGylation coating on gold nanoparticles serum protein coronaR. Nicoară, M. Ilies, C. A. Iuga, F. Loghin (Cluj-Napoca/RO), M. C. Lo Giudice, E. PoloK. A. Dawson (Dublin/IE)

P207 Protein corona characterization of a new synthesized class of PEGylated gold nanoparticlesR. Nicoară, M. Ilies, C. A. Iuga, F. Loghin (Cluj-Napoca/RO), L. M. Herda, E. PoloK. A. Dawson (Dublin/IE)

P211 Multiapproach tear proteomics analyses in glaucoma with emphasis on wound healing following trabeculectomyE. Csosz, N. Tóth, E. Deák, G. Kalló, A. Csutak, J. Tőzsér (Debrecen/HU)

P213 Searching for the ethanol-tolerant yeast: Rpn4- and proteasome-dependent yeast proteome remodeling in response to ethanol stressJ. A. Bubis, I. A. Tarasova (Moscow/RU), V. Gorshkov (Odense/DK), V. L. KarpovD. S. Spasskaya (Moscow/RU), F. Kjeldsen (Odense/DK), M. V. GorshkovD. S. Karpov (Moscow/RU)

P215 CB10Cel5: The broad-thermal-and-pH-spectrum GH5 endoglucanase suitable for sugarcane biomass degradationE. Soares Gomes Pepe, E. M. Lopes, J. C. CampanharoE. G. de Macedo Lemos (Jabotcabal/BR)

P217 Multi-omics landscape of murine pancreatic beta cell carcinoma following combined immunotherapiesJ. D’Alvise, M. A. Jarboui, B. Schörg, D. Sonanin, F. Klose, M. Röcken, S. DammeierM. Ueffing, K. Boldt, B. J. Pichler, M. Kneilling (Tuebingen/DE)

P219 Rif1 palmitoylation. The development of a high sensitivity method to identify and quantify sites of cysteine-S-acylation.D. Hess, G. A. Fontana, V. Iesmantavicius, J. Seebacher, U. Rass (Basel/CH)

P221 High-order structural characterization of monoclonal antibodies by combination of intact, middle-up and bottom-up techniques using sheathless capillary electrophoresis-mass spectrometryJ. Giorgetti, S. Nassur (Strasbourg/FR), R. Gahoual (Saint-Julien en Genevois, Paris/FR)A. Beck (Saint-Julien en Genevois/FR), P.-O. Schmit (Wissembourg/FR), E. Leize-WagnerY. N. François (Strasbourg/FR)

P223 Comparison of strategies for MS based Identification of biotinylated proteins and its application on Organelle ProteomicsS. Ahmadi, D. Winter (Bonn/DE)

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P225 Analysis of Cap G as a redox regulated proteinN. Schmidt, K. Stühler, G. Poschmann (Duesseldorf/DE)

P227 Investigation of two-step differentiation process of neurosphere to oligodendrocytes by quantitative proteomicsA. Dehghani, C. Schoor, V. Gieselmann, D. Winter (Bonn/DE)

P229 Proteomic characterization of antibody secreting cells using a bead-based western blot and a nano-LC-MS approachR. Kretz, L. Walter (Sigmaringen/DE)

P231 Discrecarin metalloprotease, a prothrombin activator from Tityus discrepans scorpion venom that supports the phylogeography of South American TityusB. Pereira, J. Brazón, J. Figueroa (Caracas/VE)

P233 Optimization of sample processing for the analysis of lysosomal proteomeS. Ponnaiyan, F. Akter, J. Singh, D. Winter (Bonn/DE)

P235 Replacing immunoassays with MS-based technology: Quantitative proteomics assays enabling deep molecular phenotyping of the mouseO. Shevchuk (Dortmund/DE), S. Michaud (Vancouver Island/CA), I. Feldmann, L. KolliparaY. Reinders (Dortmund/DE), C. Borchers (Vancouver Island/CA), A. Sickmann (Dortmund/DE)

P237 Proteomics unravels molecular basis of synapse dysfunction in MYO9A-deficiencyL. Kollipara (Dortmund/DE), E. O´Connor (Newcastle upon Tyne/GB) C. Schröder, A. Sickmann (Dortmund/DE), H. Lochmüller (Freiburg/DE; Ottawa/CA)A. Roos (Dortmund, Essen/DE)

P239 Connecting the proteome and metabolome of peritoneal dialysis effluent: Influence of dwell duration and cytoprotective interventionF. M. Wiesenhofer, R. Herzog, K. Kratochwill (Vienna/AT)

P241 Prospection and in silico analysis of a Polysaccharide Liase 8B. F. Silva de Sousa, E. Soares Gomes-Pepe, T. C. Luque Castellane, E. M. LopesE. G. de Macedo Lemos (Jaboticabal/BR)

P243 Unraveling the tissue specific Na, K-ATPase α/β-subunit combinations and elucidating their functional rolesM. Winter, S. Dalla (Hamburg/DE), J. Lohr (London/GB; Hamburg/DE)S. Dobler (Hamburg/DE)

P245 Lac 11: A new metagenomic laccase broad-thermal-spectrumN. Sarmanho Monteiro Lima, E. Soares Gomes - Pepe, E. M. LopesE. G. de Macedo Lemos (Jaboticabal/BR)

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P247 Generation and evaluation of BirA* mouse lines for cell type specific proteomicsS. Ahmadi, E. Pourbarkhordariesfandabadi, A. Egert, C. Geissen, M. Hesse, M. BreitbachK. Kimura, H. Schorle, B. Fleischmann, V. Gieselmann, D. Winter (Bonn/DE)

P249 Blood donation induced changes in the proteome of human plateletsM. Esefeld, L. Steil, V. Dhople, E. Hammer, A. Sümnig, T. Thiele, A. GreinacherU. Völker (Greifswald/DE)

P251 Redox profiling in Mesothelial Cells during experimental peritoneal dialysisR. Herzog, A. Wagner, K. Kratochwill (Vienna/AT)

P253 The mitochondrial proteome in RKIP based heart failure treatmentY. Reinders, T. Brand, L. Kollipara, K. Lorenz, A. Sickmann (Dortmund/DE)

P255 Challenges in the proteomic characterization of advanced in-vitro test systemsE. Salzmann (Sigmaringen/DE), K. Bode (Konstanz/DE), M. Templin (Reutlingen/DE)T. Brunner (Konstanz/DE), D. Stoll (Sigmaringen/DE)

P259 Ex vivo LC-MS based proteomics of the dopamine transporter applying DAT-knockout ratsC. Müller, F. Sialana, J. Wackerlig, E. Urban (Vienna/AT), G. Lubec (Salzburg/AT)T. Langer (Vienna/AT)

P261 Effects of positive airway pressure therapy on red blood cell proteome from patients with obstructive sleep apneaC. Valentim-Coelho, F. Vaz, A. Feliciano, P. Pinto, C. Barbara, D. Penque (Lisboa/PT)

P263 A new role of exogenous spliceosomal components in communication between cancer cells and chemoresistanceV. Shender, P. Shnaider (Moscow/RU), K. Anufrieva (Moscow, Dolgoprudny/RU)G. Arapidi (Dolgoprudny, Moscow/RU), I. Malianc, I. Semenov (Moscow/RU), G. StepanovE. Juravlev (Novosibirsk/RU), M. Pavlyukov (Moscow/RU), T. Grigoreva (Kazan/RU)O. Lebedeva, M. Lagarkova, V. Govorun (Moscow/RU)

P265 Evaluation of unique proteome signature of cancer cell secretomes under chemotherapy conditionP. Shnaider (Moscow/RU), K. Anufrieva, G. Arapidi (Moscow, Dolgoprudny/RU), I. MaliancM. Pavlyukov, O. Lebedeva, M. Lagarkova, V. Govorun, V. Shender (Moscow/RU)

26th to 28th of August 2019 Vienna Biocenter Campus

FIRST EUROPEAN SINGLE CELL PROTEOMICS CONFERENCE

Bogdan Budnik Harvard University, USAStefanie Hauck Helmholtz Zentrum München, GermanyJeroen Krijgsveld DKFZ, GermanyBernhard Küster TUM, GermanyAlexander Leitner ETH Zurich, SwitzerlandMatthias Mann MPI, GermanyErwin M. Schoof DTU, DenmarkNikolai Slavov NU, USARoman Zubarev KI, Sweden

• Registration Deadline:

31. Jul 2019• Registration is free of

charge but required for

attendance.

IMPORTANT

Karl Mechtler

E-Mail: [email protected]

Roman Zubarev


Claudia Ctortecka


Organization & Contact:

Con rmed Speaker:

More information can be found on our website:www.proteomics-academy.org/escp-2019

www.conventus.de

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Success throughversatile solutions

As a full-service PCO, we provide you with intelligent and innovative solutions in an advisory and implementing manner.

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26th to 28th of August 2019 Vienna Biocenter Campus

FIRST EUROPEAN SINGLE CELL PROTEOMICS CONFERENCE

Bogdan Budnik Harvard University, USAStefanie Hauck Helmholtz Zentrum München, GermanyJeroen Krijgsveld DKFZ, GermanyBernhard Küster TUM, GermanyAlexander Leitner ETH Zurich, SwitzerlandMatthias Mann MPI, GermanyErwin M. Schoof DTU, DenmarkNikolai Slavov NU, USARoman Zubarev KI, Sweden

• Registration Deadline:

31. Jul 2019• Registration is free of

charge but required for

attendance.

IMPORTANT

Karl Mechtler


Roman Zubarev


Claudia Ctortecka


Organization & Contact:

Con rmed Speaker:

More information can be found on our website:www.proteomics-academy.org/escp-2019

www.conventus.de©

Nej

ron

Phot

o / F

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ia

Success throughversatile solutions

As a full-service PCO, we provide you with intelligent and innovative solutions in an advisory and implementing manner.

66

abstractsAll abstracts will be available as a pdf-file on our website at www.eupa2019.org. Please note that all abstracts are printed as handed-in.

cateringTea, coffee, refreshments and snacks will be provided during the official program breaks within the exhibition areas (please refer to page 68). Free lunch boxes will be offered from Monday until Wednesday during the indus-trial symposia.

Certificate of attendanceCertificates of attendance may be picked up upon leaving the conference at the check-In.

GTC

General terms and conditionsPlease find all details on our website at www.eupa2019.org.

InternetA wireless internet access (wifi) is available in the main building.

general InformaTIon

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Travel grants sponsored by the DGPF and EuPAThe support of young scientist is one of the major interests of the DGPF and EuPA. There-fore, several activities are planned for young scientist at Proteomic Forum 2019 in Potsdam. Besides the EuPA Young Investigator Prize (EuPA-YIP) and the Promotion Prize of the DGPF there will be an Educational Day allowing not only young scientist to learn more about proteomics or to discuss practical questions about mass spectrometry etc. Additionally, the DGPF and the EuPA decided to give young scientist the chance to participate at the Proteomic Forum 2019 by each offering 10 travel grants (500 EUR) to young scientists.

EuPA Young Investigator prize (PhD)Young Investigator Awards (YIA) will be granted to young investigators who have started or obtained their PhD or MD degree not later than three years before the time of submission and are a member of a EuPA affiliated society and working in a European laboratory.

Promotion prize of the DGPFThe German Society for Proteome Research will award a Promotion Prize of an amount of 2,500 € to an outstanding dissertation in the field of proteomics which was finished during the last 2 years in Germany (since January 1, 2017). Here, the originality as well as the dura-tion of work and a conceivably high degree of independency will be the basis for awarding the Promotion Prize.

Poster prizes All posters can compete for the poster prize. The evaluation will take place via an online tool. All participants are allowed to vote for the three best posters. We kindly ask you to attend the award ceremony on Wednesday, March 27 for the announcement of the awardees.

MCP lectureship Dr. Marcus Bantscheff was selected to receive the MCP (Molec-ular and Cellular Proteomics) Award. His talk is scheduled for March 27, 09:30–10:00.The lectureship is an ongoing series of lectureships sponsored by the journal MCP and was chosen out of a proposed list.

aWarDs anD granTs

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We would like to thank the following sponsors for their great support.

Scienti fi c sponsorGerman Research Foundati on

Plati num sponsors

Silver sponsors

Bronze sponsors

s Ponsors anD eXHIbITors I meDIa cooPeraTIons

exhibitors Booth No.AB SCIEX Germany GmbH 9AdvaTec® Analyti cs GmbH & Co. KG 11Biognosys AG 22Bioinformati cs Soluti ons Inc. 18Bruker Daltonik GmbH 5CORTECNET 12Decondon 21Elsevier B. V. 17EURISOTOP SAS 25Evosep Biosystems 3HUPO – Human Proteome Organizati on 10I&L Biosystems GmbH 20INTAVIS Bioanalyti cal Instruments AG 7JPT Pepti de Technologies GmbH 19LI-COR Biosciences GmbH 4Matrix Science Ltd 15Merck 24MS Vision 6Olink Proteomics 8PharmaFluidics 16Promega 14SERVA Electrophoresis GmbH 21Tecan Deutschland GmbH 23Thermo Fisher Scienti fi c 13Waters GmbH 2

SponsorsIonbench SarlShimadzu Deutschland GmbH

Media cooperati onsSpringer-Verlag GmbH/Springer Spektrum, HeidelbergBioSpektrum

LJ-Verlag GmbH & Co. KG, FreiburgLaborjournal

State at printi ng

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In the center of Potsdam is a place with a holiday-atmosphere 365 days a year – the Biosphäre Potsdam. The jungle landscape of the Biosphäre Potsdam with over 20.000 beauti ful plants, many exoti c animals and an hourly thunderstorm with thunder and lightning takes you to a world far away. Your journey begins at the waterfall and conti nues on intertwined paths to the butt erfl y house and to the high-trail up in the treetops.

Date Tuesday, March 26Time 19:30–23:59Locati on Biosphäre Potsdam Georg-Hermann-Allee 99 14469 PotsdamRegistrati on fee 60 EUR

A shutt le for your way there is organized. Please meet at 18:50 at the check-in. The shutt le starts at 19:00 in front of the university.Please be aware that you need to organize your way back on your own. There is a tram (96) starti ng right in front of the building – A schedule is available at the check-in.

socIal Program

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A warm welcome to Potsdam! After many inter-esti ng talks and presentati ons it is ti me for the fi rst networking evening. We invite you to socialize with your colleagues and other delegates in a pleasant atmosphere with some food and drinks. The welcome recepti on is included in the registrati on fee.

Date Sunday, March 24Time 18:20–19:45Locati on University Potsdam Campus Griebnitzsee, Haus 6 August-Bebel-Straße 89 14482 PotsdamRegistrati on fee Included in conference registrati on

Welcome recepti�

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InDeX of PresenTIng aUTHors anD sessIon cHaIrs

aAbukhalaf, M. 42Ahmadi, S. 62Akhremko, A. 42Alahmad, A. 39Altenburg, T. 41Amado, F. 28Anagnostopoulos, T. 61Arapidi, G. 50Aravamudhan, S. 38Autret, N. 48

bBabu, M. 10, 12Banaei-Esfahani, A. 26Bandow, J. 33, 35Bantscheff, M. 10, 30Barkovits, K. 49Barth, T. 45Baum, K. 43Blankenburg, S. 47Boldt, K. 15Boosfeld, J. 56Borchers, C. 35Braccia, C. 39Brachtvogel, L. 42Bräcker, J. 49Brandt, U. 32Braun, H.-P. 14Brechlin, P. 41Bredow, C. 55Brümmer, I. 14Bubis, J. A. 62Budhraja, R. 23Bukato, O. 45

cCamoin, L. 60Cantero, P. 42Carapito, C. 23Chan, K.-y. 58Chazarin, B. 57Chilimoniuk, J. 49Chueycham, S. 52Clauwaert, J. 16Colinge, J. 17

Collins, B. C. 24Combe, C. 40Corasolla Carregari, V. 61Corbin, A. 60Csosz, E. 20, 62

DD‘Alvise, J. 62Dauly, C. 41de Almeida, A. 39, 53, 54Dehghani, A. 63de Macedo Lemos, E. G. 46, 48, 62, 63Demichev, V. 36Demmers, J. 16Dhople, V. M. 59Distler, U. 22Dittmar, D. 45Dojahn, J. 53Döllinger, J. 38Duhamel, M. 27, 58

eEckersall, D. 41, 54Eckert, M. 47Ekalaksananan, T. 40Emmanuel-Udeozor, I. 60Erdmann, J. 23Esefeld, M. 64

fFarinha Resende, A. P. 59Ferro, M. 57Fischer, L. 18Fossati, A. 61Fricke, A. L. 46Fröhlich, T. 58Fuchs, S. 36Furioso Ferreira, R. 55

gGajowski, R. 54Gandhi, T. 56Gao, Y. 38Ge, Y. 17

Gesell Salazar, M. 60Ghazala, R. 38Gloeckner, C. J. 43Gömöryová, K. 41Graham, M. 40Greco, V. 23Grossegesse, M. 59Grune, T. 23Gstaiger, M. 12Guglielmetti, C. 55

HHanáková, K. 41Hartkopf, F. 57Hartung, N. M. 42Hauck, S. 14Heinze, M. 48Hennrich, M. L. 17Hentschker, C. 23Herwig, R. 28Herzog, R. 64Hess, D. 62Hoell, L. 39Hoffmann, P. 28Hoffrogge, R. 42Hogrebe, A. 10Horvatić, A. 18, 42Hosp, F. 40Hosseini Ghaffari, M. 53Huesgen, P. 24Huguet, R. 57

IIacobucci, I. 45Ilies, M. 62Ivanova, L. 43

JJahn, O. 46Janda, M. 43Jassinskaja, M. 17Jimenez, C. 28Johnson, F. 14

72

InDeX of PresenTIng aUTHors anD sessIon cHaIrs

KKahina, B. 61Kaleja, P. 59Kashyap, P. 45Kaspar-Schoenefeld, S. 57Kelleher, N. 10, 12Kellner, R. 14, 24Kessler, B. 31Khalaf, M. 52Kikuta, K. 52Kingtong, S. 52Kipping, M. 44, 59Kiseleva, O. 42Klaeger, S. 31Kleefisch, D. 58Kniemeyer, O. 58Koch, S. 13, 41Kocijan, T. 46Kockmann, T. 54Kohlbacher, O. 27Kokot, Z. 61Kollipara, L. 63Kopczynski, D. 16Koshlan, T. 46, 60, 61Kranzler, M. 45Kratochvil, I. 44Kratochwill, K. 17Kretz, R. 63Krieg, L. 44Kroniger, T. 47Krüger, T. 39Kuchaříková, H. 45Kucklick, M. 57Kuehne, S. 56Kupcova Skalnikova, H. 42Küster, B. 30Kwon, O.-S. 38

lLackmann, J.-W. 53Langini, M. 10Leijten, N. 43Lemeer, S. 33Lemos, M. V. F. 49Lenz, C. 57

Levitsky, L. 61Lilley, K. 10, 20Lin, Y. 35Lindemann, M. 39Linden, A. 40Lochmanova, G. 56Loroch, S. 18Lottspeich, F. 18Lövfors, W. 49Lualdi, M. 48

mMajek, P. 53Malmström, J. 35Manfredi, M. 48Mann, M. 11, 13Marchiano, F. 50Marcus, K. 36Marini, F. 27, 47Martelli, C. 22Martens, L. 10, 16Mathieson, T. 42Mati-Om, J. 38Matuszewska, E. 46Matysiak, J. 47Mechtler, K. 22, 26, 27 35, 37Mehnert, M. 14Meierhofer, D. 39Memmi, S. 13, 40Mergner, J. 14Merkel, D. 39, 53Metzger, F. 49Meyer, H. 22Michalik, S. 43Mikulasek, K. 56Milentyev, A. 48Millar, H. 14Mitterer, C. 52Moez, P. 52Morgenstern, M. 33Moritz, C. 32, 44, 58, 61Morrice, N. 55Morris, N. 53

Mosen, P. R. 49Moshkovskii, S. 54Moutaoufik, M. T. 52Müller, C. 64Müller, G. 59Müller, S. 60Muth, T. 54

nNdiaye, S. 57Neves, S. 49Nicoară, R. 62Nilsson, P. 35Nita-Lazar, A. 44Nukpook, T. 54

oOlsen, J. 30Ortseifen, V. 47

PPan, K.-T. 42Pankert, P. 43Pecankova, K. 53Peffers, M. 54Pennington, S. 10, 12, 20Pereira, B. 63Petrera, A. 18Petzoldt, S. 40Pientong, C. 42Pierros, V. 46Pimkova, K. 23Pineau, C. 10, 20, 26Piras, C. 33Pitt, A. 32Poliakh, I. 41Pollack, L. 52Polten, F. 55Ponnaiyan, S. 63Potesil, D. 58Prianichnikov, N. 54Prust, N. 40Pschibul, A. 58Pythoud, N. 14

general InformaTIon

73

rRadau, S. 57Ralser, M. 20, 27Ramberger, E. 15Ramšak, B. 56Rana, R. 52Raposo de Magalhães, C. 53Reimann, L. 15Reinders, Y. 64Reis-de-Oliveira, G. 45Reiter, L. 22, 36Resetar-Maslov, D. 26, 27Reusch, D. 13Rexroth, S. 21, 59Rhode, H. 52Ribeiro, D. M. 56Rodriguez, H. 12Rodríguez-Ortega, M. J. 14Rogowska-Wrzesinska, A. 23Rosenblatt, M. 60Rosenblatt, M. 60Rossetti, C. 57Ruhaak, R. 22

sSailer, C. 17Salzet, M. 45Salzmann, E. 64Sarmanho Monteiro Lima, N. 63Savitski, M. 20Schallert, K. 55Schäpe, S. 22Schiebenhöfer, H. 27Schiffmann, C. L. 59Schlüter, H. 45Schmidt, A. 40Schmidt, N. 10, 63Schmit, P.-O. 37, 62Schmitt-Ulms, G. 17Schnatbaum, K. 55Schork, K. 41Schwarz, J. 50Schweitzer, T. 35Sedo, O. 56

Selbach, M. 10, 30Senges, C. 33Serrano, L. 20Shan, B. 16Shender, V. 64Shevchuk, O. 63Shibata, T. 39Sickmann, A. 12Sigloch, F. C. 47Singh, J. 48Sinz, A. 10, 24Smagul, A. 47Soggiu, A. 24Solovyeva, E. 54Stoychev, S. 38Stühler, K. 16Suarez-Artiles, L. 43Sundqvist, N. 49Sunny, T. 40Surinova, S. 17Surmann, K. 35Swiatly-Blaszkiewicz, A. 46

TTeferedegn, E. 38Tenzer, S. 49Thanormjit, K. 38Tholey, A. 44Trappe, K. 56Trautwein-Schult, A. 58Trifonova, O. 36, 61Tsangaris, G. 16Turewicz, M. 27

UUeffing, M. 31Uliana, F. 48Urbani, A. 17Urizar-Arenaza, I. 47Urlaub, H. 13, 31

VValentim-Coelho, C. 64Van Damme, P. 23

van Eyk, J. 12Verbruggen, S. 39Virreira Winter, S. 47Völker, U. 11, 33, 37von Törne, C. 43Voß, J. 48

WWaidelich, D. 53Warnken, U. 53Warscheid, B. 12Weibchen, G. 21, 44, 59Wenske, S. 56Werner, T. 18Wielsch, N. 44Wiesenhofer, F. M. 63Willforss, J. 56William Norbert, K. T. 38Wilson, J. 22Winter, D. 64Winter, M. 63Wirtz, A. 61Wittig, I. 33Witzke, K. 46Wohlrab, S. 46Wollscheid, B. 13, 20Wulf, A. 24

XXiao, Y. 38Xuan, Y. 36

YYasmeen, F. 52Yates, J. 10, 37Yu, K. 55

zZauber, H. 45Zdrahal, Z. 41Zhong, Q. 28Zorina, E. 4

74

noTes

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The timsTOF Pro with PASEF technology delivers revolutionary improvements in scan speed, coupled with enhanced specifi city and high sensitivity. It does so while simultaneously maintaining ultra-high resolution for both precursors and MS/MS spectra. This unique performance quintet gives scientists the tools to dig deeper into the complex biology of the deep proteome.

Screen, Confi rm and Quantify

Near 100% duty cycle using dual TIMS technology

Unrivalled MS/MS speed >100Hz

Discover more proteins using PASEF

For more information please visit www.bruker.com/timstofpro

TIMS-QTOF MSInnovation with Integrity

timsTOFThe New Standard for Shotgun Proteomics

Powered by PASEF

For research use only. Not for use in diagnostic procedures

timsTOF Pro_A5_08-2018.indd 1 07.03.2019 16:56:41

75

Powered by PASEF

The timsTOF Pro with PASEF technology delivers revolutionary improvements in scan speed, coupled with enhanced specifi city and high sensitivity. It does so while simultaneously maintaining ultra-high resolution for both precursors and MS/MS spectra. This unique performance quintet gives scientists the tools to dig deeper into the complex biology of the deep proteome.

Screen, Confi rm and Quantify

Near 100% duty cycle using dual TIMS technology

Unrivalled MS/MS speed >100Hz

Discover more proteins using PASEF

For more information please visit www.bruker.com/timstofpro

TIMS-QTOF MSInnovation with Integrity

timsTOFThe New Standard for Shotgun Proteomics

Powered by PASEF

For research use only. Not for use in diagnostic procedures

timsTOF Pro_A5_08-2018.indd 1 07.03.2019 16:56:41

program - eupa 2019 · of complex proteomics data, in a hands on workshop performed by experts in...

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