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PROGRAM BOOK
INTERNATIONAL SEMINAR AND
WORKSHOP ON ADVANCED CELLULAR
AND MOLECULAR BIOLOGY TECHNIQUES
6 - 7 JULY 2017
INTEGRATED BIOMEDICAL LABORATORY
FACULTY OF MEDICINE
UDAYANA UNIVERSITY
2
FOREWORD
Dear colleagues
Welcome to the Faculty of Medicine Udayana University
Welcome to the International Seminar and Workshop on Advanced Cellular and Molecular
Biology Techniques
As we all know, cellular and molecular biology have an important theoretical and practical
contribution in the field of science, particularly health and medicine. Cellular and molecular
techniques had been developed very quickly in the field of agricultural biology, farm biology,
pharmacy, veterinary and medicine. Both of them have a great contribution in explaining the
basis of life on earth and how to use that knowledge for human wellbeing.
This seminar held as continuation of our understanding about important role of biomedical
science. We hope there will be a great discussion about recent advances in biological science
and its applications related with health science and disease. Highly reputable foreign and
native speakers are invited to share their knowledge about the advancement in biomolecular
science and techniques. There are free papers as well as poster presentations from many
researchers in Indonesia that have a great contribution on the ongoing discussion on
molecular and cellular biology topics.
The seminar is commenced with a workshop on theme “Detection of gene
polymorphism” and “Choosing a Proper ELISA Method” as a part to learn methods that are
commonly used in molecular and cellular biology areas.
This book program provides you schedule of the event, abstract and biography of invited
speakers and abstract of free papers and posters.
We would like to express our gratitude to all sponsors. Those sponsors are an invaluable part
of our work. Their support helps us generate the palpable energy of the seminar and
workshop.
We hope you have a fruitful discussion and an enjoyable seminar and workshop!
Ni Nyoman Ayu Dewi
Chair
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TABLE OF CONTENT
Page
Foreword 2
Table of Content………………………………………………………………. 3
Schedule ……………………………………………………………………..
Workshop 4
Seminar 5
Paralel Oral Presentation 6
Poster Presentation 7
Abstract of Invited Speaker
Cellular and Molecular Biology Techniques:
From basic to advanced methods (Sangkot Marzuki) 9
Over-expression of Proteins in E.coli (Wen-Chien Lee 10
Hepatitis B Drug Resistance: Molecular Technique for
Detection (I Dewa Nyoman Wibawa) 11
Novel Understanding of Cellular and Molecular Basis of
Sepsis Pathophysiology: A Proposal of New Treatment Strategy
(Masahiro Nishibori) 13
Next Generation Sequencing Application in Medicine
(Joanne Ngeow) 14
Novel Pathogen Identification by Matrix Assisted
Laser Desorption/Ionization-Time of Flight
(Marut Tangwattanachuleeporn) 15
Stem Cell Therapy as an Alternative in Anti-Aging and
Regenerative Medicine (Indra Bachtiar) 16
Curriculum Vitae of Invited Speaker 17
Abstract of Oral Presentation 25
Abstract of Poster Presentation 51
Committee 67
4
SCHEDULE
WORKSHOP
Thursday, 6th July 2017
Time Activity Speaker/PIC
8.00-8.30 Registration Committee
8.30-9.15 Polymorphism and PCR Ayu Dewi/Taufik
9.15–9.30 BREAK
9.30-10.30 Hands on DNA purification Team
10.00-10.30 ELISA Step 2 Team
10.30-11.00
Hands on PCR (Polymorphism
Detection)
Team
10.30-11.00 Hands on DNA concentration
measurement
Taufik
11.00-11.45 ELISA Wande
12.00-12.15 ELISA Step 3
12.15-13.00 LUNCH BREAK
13.15-13.30 ELISA Step 4 Team
14.00-14.15 ELISA Step 5 Team
14.15-15.15 Electrophoresis Team
15.00-15.15 ELISA reading Team
15.15-15.30 BREAK
15.30-16.15 Sequencing analysis Ayu Dewi
16.15-selesai Discussion and closing Team
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SCHEDULE
SEMINAR
Friday, 7th July 2017
Time Venue Person In
Charge
07.30-08.30 Registration Secretariat
Division
08.30-09.00 Pembukaan
- Welcome Dance
- Welcome speak from Chair
- Welcome speak and opening by Rector
Event/Seminar
Division
09.00-10.00 Keynote speaker
Prof. Dr. Sangkot Marzuki (Cellular and
Molecular Biology Techniques : From basic to
advanced methods)
Moderator ( dr.
Mariadi, Sp.PD)
10.00-10.30 Coffee break
10.30-11.00 Dr. Joanne Ngeow Yuen Yie, MBBS,M.Med,
MRCP,FAMS,MPH (Next Generation
Sequencing Application in Medicine)
11.00-11.30 Prof. Dr.dr I Dewa Nyoman Wibawa, SpPD-
KGEH (Molecular biology techniques in
clinical setting)
11.30-12.00 Indra Bachtiar, Ph.D (Stem Cell Technology)
12.00-12.30 Panel Discussion
12.30-14.00 ISHOMA
14.00-14.30 Prof. Wen-Chien Lee, PhD (Over- Expression
of Proteins in E.coli)
Moderator (dr.
Komang Januarta
14.30-15.00 Prof. Masahiro Nishibori, M.D, PhD (Novel
Understanding of Cellular and Molecular basis
of Sepsis Pathophysiology: a proposal of new
treatment strategy
15.00-15.30 Asst. Prof Marut Tangwattanachuleeporn, Dr.
rer.nat (Novel pathogen Identification by matrix
assisted laser decorption/ionization-time of
flight
15.30-16.00 Panel Discussion
16.00-17.00 Parallel Discussion
18.00-finish Closing
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PARALLEL ORAL PRESENTATION
Time and Place Speaker Person in Charge/Moderator
Time :16.00-17.00
Place : Ruang Sidang FK
(Health)
1. AA Mas Putrawati
Triningrat
2. Bagus Komang
Satriayasa
3. Yuliana Heri Suselo
4. Dordia Anindita
Rotinsulu
5. Silvi Dwi Anggraini
Moderator : dr. I Kadek
Swastika, M.Kes
Committee : I Made
Suardana
Time : 16.00-17.00
Place : Ruang Histologi
(Health)
1. Mahadewa Tjokorda BG
2. Made Kurnia Widyastuti
Giri
3. I Nyoman Suarsana
4. Elysanti Dwi Martadiani
5. Desak Made Wihandani
Moderator : Dr. dr I
Nyoman Wande, Sp.PK
Committee : I Gede
Wiranatha
Time : 16.00-17.00
Place : Ruang Bahasa
(Health)
1. Luh Putu Iin Indrayani
Maker
2. I WP Sutirta Yasa
3. I Made Jawi
4. I Kadek Swastika
5. Ni Wayan Tianing
Moderator : dr. I Wayan
Surudarma, M.Si
Committee : I Dewa Ketut
Alit Sumarsana
Time : 16.00-17.00
Place : Ruang kelas 401
Agriculture, Nutrion, And
Environment
1. Sheila Simanjuntak
2. I Ketut Berata
3. Ida Bagus Made
Artadana
4. Lili Anggaini
5. I Gusti Ngurah Gede
Putra
6. Sang Gede Purnama
7. Vabera Maslami
Moderator : dr. I Gusti
Kamasan Nyoman Arijana,
M.Si, Med
Committee : I Made Arjana
Note :
- Time for presentation is @ 8 min (presentation + discussion)
- Please give your presentation to the committee when you register at the registration
desk
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POSTER PRESENTATION
No. Abstract Title First Author
1
THE EFFECT OF CACAO BEANS
EXTRACTS ADMINISTRATION ON
SOD AND OX-LDL CONCENTRATION IN OXIDATIVE STRESS
CONDITIONED RAT
Dewi
Wiryanthini IA
2
ANALYSIS OF FOOD USAGE AND NUTRITIONAL STATUS IN RURAL
AREA IN BALI PROVINCE,
INDONESIA
Kadek Tresna
Adhi
3
THE RISK OF CERVICAL
ADENOCARCINOMA IN PATIENTS
INFECTED WITH HUMAN
PAPILLOMAVIRUS TYPE-18
I Gusti Ayu Sri
Mahendra Dewi
4
THE PROFILE OF EPIDERMAL GROWTH FACTOR RECEPTOR
MUTATION DETECTED FROM
CYTOLOGIC SLIDES OF LUNG ADENO CARCINOMA PATIENTS IN
SANGLAH HOSPITAL, BALI
Ni Wayan
Winarti
5
COMBINATION OF HIGHER DOSE
METFORMIN AND LOWER DOSE CISPLATIN INCREASES APOPTOSIS
IN CERVICAL CANCER CELLS LINE
Ratih Dewi Yudhani
6
S GENE MUTATIONS OF HEPATITIS
B VIRUS IN HEPATITIS B CHRONIC PATIENTS AFTER 12 WEEKS OF
TELBIVUDINE TREATMENT
Yenti Purnamasari
7
ETHANOLIC EXTRACT OF THE
POWDER OF RED EARTHWORM (Lumbricus rubellus) OBTAINED FROM
SEVERAL ORGANIC FARMLANDS IN
BALI, INDONESIA: ANALYSIS OF TOTAL PHENOLIC CONTENT AND
ANTIOXIDANT CAPACITY
Ni Wayan Sucindra Dewi
8
Pharmaco-ethological and Hippocampal
CA1 Region Neurohistological Study of Sertraline Effects on Single-prolonged
Stress-induced Rodent Model of PTSD
Agung Nova Mahendra
9
The anticancer activity of (e)-1-(4’-aminophenyl)-3-phenylprop-2-en-1-on
against DMBA-induced mammary cancer
in Sprague Dawley rat through the regulation of microRNA-21 expression.
Ida Ayu Ika Wahyuniari
10
Preparation and in vitro characterization of self-nano emulsifying system of C-
phenylcalix-[4]-resorcinaryl octacinnamate
and C-methylcalix-[4]-resorcinaryl octabenzoate as ultraviolet absorbers
Indrayani Agung Wiwiek
11
Supplementation 2000mg and 1000mg of Linoleic Acid and Alfa Linolenic Acid
Delayed Prediabetic State in Balinese
Young Obese Women: a Randomised Clinical Trial
I W Weta
No Abstract Title First Author
12
CYP2E1 GENOTYPE AND
TRANSAMINASE LEVEL OF TUBERCULOSIS PATIENTS
RECEIVING FIXED DOSE
COMBINATION OF ANTITUBERCULOSIS
I Gusti Ayu
Artini
13
Determinants of Post Traumatic
Stress Disorder (PTSD) Among Traffic Accident Victims in Bali -
Indonesia
Putu Aryani
14
Detection of Citrus Vein Phloem
Degeneration (CVPD) Disease and the Quality of Healthy Fruits in
Nutrient Deficiency of Citrus
I Gede Putu Wirawan
15 Mutation Identification of RpoB Gene from MDR-TB Isolates at
Sanglah Hospital Denpasar, Bali
Dwija, Ida Bagus Nyoman
Putra
16
Hypoglycaemic effects of
zyzygum polyanthum leaves extract on Alloxan-Induced
Through Decrease Of Blood
Glucose And 8-Hydroxy-2 Deoxyguanosine (8-OHdG) In
Hyperglycemic Wistar Rats
Sri Wahjuni
8
ABSTRACT OF INVITED
SPEAKERS
9
CELLULAR AND MOLECULAR BIOLOGY TECHNIQUES: FROM BASIC TO
ADVANCED METHODS
Prof. Sangkot Marzuki
Akademi Ilmu Pengetahuan Indonesia (AIPI)
Indonesian Academy of Sciences
ABSTRACT
10
OVER-EXPRESSION OF PROTEINS IN E. COLI
Wen-Chien Lee
Department of Chemical Engineering, National Chung Cheng University, Minhsiung 621,
Taiwan
ABSTRACT
The techniques for producing recombinant proteins at a high level in Escherichia coli are
well established. Normally, over-expression is achieved by the integration of the nucleotide
sequence that encodes a protein of interest into a high copy-number plasmid containing the
strong and regulatable promoter and then activated by the addition of an inducer. However, as
a recombinant protein is over-expressed in E. coli, aggregation of polypeptide chains to form
insoluble inclusion bodies (IBs) has been frequently observed. In this presentation, previous
studies on the mechanism of IBs formation and possible sources of cytoplasmic IBs are
reviewed, and the methods to improve the solubility of the over-expressed proteins are
discussed. IBs are conventionally believed to be formed by the irretrievable deposition of
partially folded or improperly folded intermediates and they are thus biologically inactive.
However, biologically active IBs have been observed for many enzymes including
galactosidases, reductases, oxidases, kinases, phosphorylases, and aldolases when over-
expressed in E. coli. The IBs containing proteins with higher conformational quality and
enzymatic activity are thus of great interest. The strategies that can create favorable cellular
conditions for producing active IBs for using as biocatalysts will be thoroughly discussed.
11
HEPATITIS B DRUG RESISTANCE:
MOLECULAR TECHNIQUE FOR DETECTION I Dewa Nyoman Wibawa
Gastroentero-hepatology Div., Dept.of Internal Medicine,
Udayana Univ., School of Medicine/
Sanglah General Hospital.
ABSTRACT
In the treatment of chronic hepatitis B in the past, substantial advances have been made. Until
recently, approved treatments for chronic hepatitis B include 2 formulations of interferon and
4 nucleos(t)ide analogues (NAs). Sustained viral suppression is rarely achieved after
withdrawal of a 48-week course of NA therapy, necessitating long, and in many cases,
indefinite treatment with increasing risk of development of drug resistance. Antiviral
resistance and poor adherence are the most important factors in treatment failure of hepatitis
B.
The occurrence of antiviral drug resistance is depends on, what we call as the 4 golden rules
of drug resistance, viral mutation frequency, the intrinsic mutability of the antiviral target
site, the selective pressure exerted by that drug, and the magnitude and rate of virus
replication.
The particular principles of antiviral drug resistance are: single and double mutants preexist;
this is because viral replication generates diversity. Resistance may fade, but not disappear;
that is, when you stop therapy, the resistant virus does not disappear, it is just replaced into a
minor compartment or a minor quasi species; then, when that therapy is reinstigated, it
reappears. So it may fade, but it will not disappear. Partial suppression promotes resistance,
no replication equals no resistance. The polymerase is structurally flexible. Patient
compliance is important.
Monitoring the emergence of antiviral resistance becomes increasingly important especially
with the expanding repertoire of nucleoside/nucleotide analogs for anti-HBV therapy. Severe
hepatitis exacerbations associated with resistance mutations further complicate the picture of
HBV infection. HBV replicates via an RNA intermediate. The lack of proofreading ability of
the RT (HBV polymerase) is attributed to the increased mutational rates in HBV. The
emergence of mutants resistant to antiviral therapy is associated with reduced treatment
efficacy, treatment failure or virological breakthrough.
Genotypic resistance refers to the detection of mutations in HBV DNA that correspond to
changes in amino acid sequence in the reverse transcriptase region of the HBV genome that
have been shown to confer resistance using phenotypic assays, while phenotypic resistance
refers to the decreased susceptibility of HBV polymerase to an antiviral agent in vitro.
Genotypic and phenotypic assay is use for determining HBV drug resistance.
Phenotypic assays for the detection of antiviral resistance are based on the demonstration of
decreased susceptibility of a replication mutant clone to the antiviral agent in vitro as
compared with the wild-type strain. Phenotypic assays offer the advantage of directly
measuring drug susceptibility as well as the cumulative effect of multiple mutations. The
concentration of the antiviral agent required to reduce the replication of the HBV clone
studied to 50% replication is referred to as the IC50. An increase in the concentration of the
antiviral agent required as determined by the IC50 is expressed as 'fold change'.
Genotyping and phenotyping of clinical isolates of HBV is routinely done in many
laboratories worldwide and the results can be expected to aid in design of therapeutic
strategies that maximize the benefit from new antiviral drugs. Resistance testing will also
make an increasing contribution to patient management, especially for individuals who have
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already failed treatment and those who have known risk factors associated with more rapid
emergence of drug resistance.
The availability of advances in methods for HBV DNA quantitation and the early
identification of drug resistance in HBV have been particularly useful in the management of
chronic HBV infection. The decision of switching to appropriate alternative therapies
following early identification of drug resistance will improve the outcome of therapeutic
interventions. The need to better understand the clinical outcome and to minimize the
morbidity in patients with chronic HBV will drive the development of newer molecular
methods.
13
NOVEL UNDERSTANDING OF CELLULAR AND MOLECULAR BASIS OF
SEPSIS PATHOPHYSIOLOGY: A PROPOSAL OF NEW TREATMENT
STRATEGY
Masahiro Nishibori
Department of Pharmacology
Okayama University Graduate School of Medicine, Dentistry
and Pharmaceutical Sciences
http://www.okayama-u.ac.jp/user/pharmaco/
ABSTRACT
Sepsis is a systemic inflammatory response syndrome (SIRS) associated with infection. The
pathogenesis of sepsis includes the disturbance of blood-vascular homeostasis, which may
cause multiple organ failure, circulatory shock, and disseminated intravascular coagulation,
leading to high mortality. The proinflammatory cytokine response in the acute phase may be
triggered by the constituents of invading pathogens and tissue damage-associated molecular
patterns, accompanied by the activation of vascular endothelial cells, a pivotal step for
inducing the migration of leukocytes into inflammatory sites with pathogen invasion. A
recent study suggested that neutrophil adhesion on vascular endothelial cells may trigger
platelet aggregation and immunothrombus formation in septic acute respiratory distress
syndrome (ARDS). Thus, circulating neutrophils may play important roles in the
pathogenesis of septic conditions in addition to infiltrating neutrophils. However, the
uncontrolled activation of neutrophils has not been examined in detail due to methodological
limitations.
Recently, we found a novel mechanism of activation of circulating neutrophils and vascular
endothelial cells in septic condition that has shed light on our understanding of septic
pathogenesis. We identified a plasma protein that maintains the circulating neutrophils and
vascular endothelial cells quiescent and decreases markedly during sepsis. Based on these
findings, I will propose a novel therapy for sepsis and multiple organ failure in clinic.
14
NEXT GENERATION SEQUENCING APPLICATION IN MEDICINE
Joanne Ngeow
Head, Cancer Genetics Service
Division of Medical Oncology
National Cancer Centre Singapore
From the vantage point of 2017, it is difficult to imagine a time when cancer was not widely
accepted as a genetic disease, in the most basic sense of being caused by alterations in the
structure and function of genes. Indeed, it was not until the second half of the 20th century
that the heritable nature of common cancers started to be widely accepted. Over the decades
since then, genealogic and epidemiologic observations set the stage for a burst of scientific
activity in the 1990s, when developments in positional cloning facilitated the discovery of
genes responsible for the most common cancer predisposition syndromes, and for several less
common ones. These discoveries provided invaluable insights in the biology of not only
inherited cancer but also sporadic disease and led the way for cancer genomics research and
gene-directed management and treatment. Currently, over 400 hereditary cancer
susceptibility syndromes have been described, the majority of which are inherited in an
autosomal dominant manner. Although many of these are rare syndromes, they are thought to
account for at least 5–10% of all cancer, amounting to a substantial burden of morbidity and
mortality in the human population. It is increasingly important that clinicians from all fields
stay abreast of the cancer and non-cancer manifestations of these syndromes. We will review
how next-generation sequencing has revolutionized medical practice allowing us to tailor our
treatments and management according to the genomic profile of patients. We will review the
promise as well as the challenges associated with next generation sequencing in clinical
practice.
15
NOVEL PATHOGEN IDENTIFICATION BY MATRIX ASSISTED LASER
DESORPTION/IONIZATION-TIME OF FLIGHT
Marut Tangwattanachuleeporn
Medical Technology Unit, Faculty of Allied Health Sciences,
Burapha University, Chon Buri, Thailand.
ABSTRACT
In few years ago, matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) has
emerged as a fantastic tool for microorganism identification. The principle of this tool is the
analysis of mass spectrometry in ribosomal proteins, which are commonly abundant in the
microbial cell. Then, the mass spectra of ribosomal proteins are analyzed by software and
compared with profile databases of microorganisms. Indeed, this technique has become a
standard method in clinical diagnostic microbiology. Moreover, this technique can be applied
for direct detection of pathogen from clinical specimens (e.g. blood, urine and cerebrospinal
fluid), discovery of antibiotic-resistant pathogens without drug susceptibility test, and
bacterial and fungal typing in epidemiology including generation of phyloproteomic trees.
Microbial identification by MALDI-TOF mass spectrometry is rapid, sensitive, robust,
automatable, and economical in terms of both labor and money. Ultimately, this presentation
will focus in comparison with conventional routine methods and share an idea about its
application in the future.
16
STEM CELL THERAPY AS AN ALTERNATIVE IN ANTI-AGING AND
REGENERATIVE MEDICINE
Indra Bachtiar
Stemcell and Cancer Institute (SCI), PT Kalbe Farma Tbk
Jl Ahmad Yani no 2, Pulomas, Jakarta 13210, INDONESIA
ABSTRACT
Aging is an inevitable physiological change. It is a multifactorial process characterized by a
progressive loss of physiological integrity that leads to impaired function and increased
vulnerability to death. Molecular inflammation is an important biological component of
aging, and monitoring the molecules that mediate inflammation may be useful to assess the
aging processes. Specifically, monitoring blood levels of pro-inflammatory cytokines such as
tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) may give us the means to better
understand the aging process. Furthermore, hallmarks of senescent cells include cellular
DNA damage, mitochondrial dysfunction leading to increased reactive oxygen species (ROS)
and decreased ATP, the production of proinflammatory cytokines, telomere shortening, the
trigger of stem cell depletion and cell senescence. Currently, anti-aging therapy with stem
cells as a regenerative medical treatment is proposed as the most effective way to delay
senescence. Stem cell transplantation is generally considered as a highly promising candidate
method for regenerative applications because stem cells possess a high proliferative capacity
and have the potential to differentiate into other cell types. Moreover, previous studies
suggest that accumulated metabolic stress and impaired function of adult stem cells in vivo
are critical for the initiation and development of aging and aging-associated degenerative
diseases. However, understanding the process of stemcell is crucial for efficient expansion
protocols to generate a large number of cells based on traditional culture technic that is
necessary for successful treatment. The aim of this presentation is to take a deep insight into
significant aspects of stemcell-based therapies and to highlight practical limitations that
might impair a safe and efficient clinical application.
17
CURICCULUM VITAE OF
INVITED SPEAKERS
18
Sangkot Marzuki
Professor Sangkot Marzuki is President of the Indonesian Academy of Sciences, and was
Director of the Eijkman Institute for Molecular Biology in Jakarta from 1992-2014. His
scientific interest has been in the biogenesis of energy transducing membranes, and related
human genetic disorders; an interest he developed during his 17 years as medical faculty
member of Monash University in Australia, before moving to Indonesia in 1992 to rebuild
the Eijkman Institute, where he extended this interest to encompass human genome diversity
and infectious diseases. For his scientific contribution, he received a higher doctorate from
Monash University (1998), and awarded doctor honoris causa from Utrecht University
(2006), and doctor of science honoris causa from the University of Queensland (2010).
Professor Marzuki graduated as a medical doctor from the University of Indonesia in 1968,
obtained his M.Sc. from Mahidol University in Bangkok (1971), and Ph.D. from Monash
University (1975). He has held honorary appointments as a Professor of Medicine at Monash
University (Melbourne), Professor of Medical Sciences at the University of Indonesia in
Jakarta, Professor of Biochemistry and Molecular Biology at the University of Queensland,
and Professor of Medicine at Sydney University. He served in the Indonesian National
Research Council (1994-2004) and Higher Education Council (1996-2000; 2003-2008), as
Governing Council Member of Asia-Pacific International Molecular Biology Network (1998-
2008), and chairman of the Asia Pacific Network of Human Geneticists (1995-2005).
19
Wen-Chien Lee
Dr. Wen-Chien Lee is professor in Department of Chemical Engineering and Director of
Systems Biology and Tissue Engineering Research Center at National Chung Cheng
University. He received his B.S. and M.S. in chemical engineering from National Taiwan
Institute of Technology and Ph.D. from Purdue University (1989). He has served as a
visiting professor in Cornell University (USA, 2001) and Kasetsart University (Thailand,
2014) and chairman of Department of Chemical Engineering at National Chung Cheng
University. In past three years, he was the President of the Biotechnology and Biochemical
Engineering Society of Taiwan (BEST) and Deputy Secretary General of the Asian
Federation of Biotechnology (AFOB). Now he is serving as the Honorable President of
BEST to continue his service to biotechnology community. He has published 100
international journal papers and book chapters. His current research interests are proteomics,
biomagnetic particles, production of proteins and biomass-based chemicals.
20
I Dewa Nyoman Wibawa
First Name : Prof. Dr. dr. I Dewa Nyoman Last Name: Wibawa, Sp.PD-KGEH
(with degrees)
Date of Birth : November 17th 1952 Married to: dr. Ni Ketut Candra Wiratmi, Sp.S
Office/Institution : Gastroentero-Hepatology Division, Department of Internal Medicine.
Medical Faculty Udayana University/Sanglah General Hospital
Office Address : Jl. Diponegoro, Denpasar Bali
Telephone : 0361-244177 Fax: 0361-244177
E-mail Address : [email protected]
Home Address : Jl. Sekar Tunjung II/1 Denpasar Bali
Current Position : Chief of Gastroentero-Hepatology Division, Department of Internal
Medicine. Medical Faculty Udayana University/Sanglah General Hospital
Academic Appointment and Activities:
- Medical Doctor, Udayana University (1979)
- Specialty in Internal Medicine, Diponegoro University (1986)
- Consultant in Gastroentero-Hepatology, Indonesia Association of Internal Medicine
(1997)
- PhD, University of Airlangga (2000)
Professional Activities:
- Doctor in Sanglah General Hospital
- Lecturer in Medical Faculty Udayana University
- Head of Study Program of Internal Medicine, Medical Faculty, Udayana Universitiy
Awards and Qualifications:
- Medika Award (1999)
Society Memberships:
- Indonesian Association of Medical Doctor
- Indonesian Association of Internal Medicine.
- Indonesian Association for the Study of the Liver Diseases.
- Indonesian Association of Gastroenterology.
- Indonesian Association of Digestive Endoscopy
21
Masahiro Nishibori
Sex : Male
Birthday : March 16, 1956
Nationality : Japanese
Current Position: Professor, Department of Pharmacology, Okayama University Graduate
School of Medicine, Dentistry and Pharmaceutical Sciences
Address : 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan
Communication: Phone/Fax: +81-86-235-7140
E-mail: [email protected]
Education:
1971-1974 Hikone-higashi High School in Shiga Prefecture
1974-1980 Okayama University Medical School
1985 PhD (Okayama University Graduate School of Medicine)
Employment
1980-1988 Research Associate of Okayama University Medical School
1988-1995 Assistant Professor of Okayama University Medical School
1990-1992 Visiting Scientist of Manitoba Institute of Cell Biology, Manitoba, Canada
1995-2001 Associate Professor of Okayama University Medical School
2001- Professor of Okayama University Graduate School of Medicine, Dentistry, and
Pharmaceutical Sciences
Awards
1983 Yuki Memorial Prize of Okayama Medical Society
1990 Uehara Memorial Research Fellowship (The Uehara Memorial Foundation)
2009 21st Century Invention Award (Japan Institute of Invention and Innovation)
2010 Okayama Prefectural Culture Prize (Academic Category) (Okayama Prefecture)
2012 Bio Business Japan Award 2012 (Saito-Award) (Osaka Prefecture)
2012 Sanyo Newspaper Award (Academic field)
Research Fields: Pharmacology, Drug Development
Academic Activities:
Editor-in-Chief of Journal of Okayama Medical Association (2009-2015)
Associate Editor of Journal of Pharmacological Sciences (2006-2008; 2015 - )
Representative of Forum on Drug Research in Okayama (FDRO) (2004 - )
Member of Japanese Pharmacological Society, Japanese Neuroscience Society,
Japanese Immunology Society, and Society for Neuroscience (USA)
Invited Lectures:
2010 Special Invited Lecture in Annual Meeting of Japanese Pharmaceutical Society
2010 The Fourth International HMGB1 Symposium
2012 Japanese Pharmaceutical Society, Drug development symposium
2012 The 22th Brain Blood Vessel Symposium
2011 Special Invited Lecture in Japanese Neurosurgery Meeting
2014 Special Invited Lecture in Japanese Anesthesiology Meeting
2012 The 22th Brain Blood Vessel Symposium
Publications: 150 peer-reviewed papers
22
Joanne Ngeow
Head, Cancer Genetics Service
Division of Medical Oncology
National Cancer Centre Singapore
11 Hospital Drive
Singapore 169610
Dr Joanne Ngeow, MBBS, MRCP, MPH is Senior Consultant,
Division of Medical Oncology at the National Cancer Centre
Singapore and Assistant Professor with the Oncology
Academic Clinical Program, Duke-NUS Graduate Medical
School. Dr Ngeow currently heads the Cancer Genetics Service at the National Cancer Centre
Singapore with an academic interest in hereditary cancer syndromes and translational clinical
cancer genetics.
She was awarded consecutive fellowships by the National Medical Research Council and the
Ambrose Monell Foundation to complete formal clinical and bench training in Cancer
Genomic Medicine at the Genomic Medicine Institute, Cleveland Clinic, Ohio. Dr Ngeow’s
research focuses on understanding how gene-environmental interactions predisposes to
cancer initiation and progression.
23
Marut Tangwattanachuleeporn Burapha University, Division of Microbiology, Department of Medical
Technology, Faculty of Allied Healt Sciences, 169 Longhadbangsaen
Road, Saensuk District, Maung, Chonburi, 20131, Thailand
Marut Tangwattanachuleeporn is a microbiologist currently holding an
Assistant professorship for Microbiology at Burapha University, Chon
Buri, Thailand. He got master degree in medical microbiology with the
highest GPA in the class as 3.94 from Chulalongkorn University,
Thailand and earned Ph.D. degree in Biology from Goettingen University, Germany. In 2011
– 2013, he was a representative for Southeast Asia of the Young ISHAM (the International
Society for Human and Animal Mycology, one of the biggest fungal society). His current
research focus on the prevalence of pathogenic fungi such as Aspergillus spec.,
dermatophytes, or Cryptococcus spec. in the environment as potential sources of infection as
well as monitoring of their antimycotic drug resistance. He had 12 international publications
and 7 national publications (in Thai). Now, he has an administrative position in Burapha
University as Assistant president for research affairs.
24
Indra Bachtiar
EDUCATION
1. Postdoctoral at Laboratorium of Biophysical Chemistry, National Heart, Lung, and Blood Institute (NHLBI), National Institute of Health (NIH), Bethesda, Maryland, USA (2002-2005)
2. Ph.D., Department of Pharmaceutical Molecular Biology, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba, Aramaki, Aoba-ku, Sendai 980-8578, Japan (1998-2002)
PROFESSIONAL POSITIONS Head of Stemcell R&D/Production and Commercialization, REGENIC Lab, PT
Bifarma Adiluhung, Kalbe Farma TbK Principal Investigator at Stem Cell and Cancer Institute Indonesia -SCI-
Indonesia Reviewer at Elsevier Publisher, Bentham Science Publisher, Springer and
BMC-Proteome Science PATENT AND AWARDS 1. MONBUSHO Scholarship, Japan, 1998. 2. Intramural Research Training Award, IRTA, National of Heart, lung and Blood
Institute, NIH, Bethesda, USA, 2003 3. International Stemcell Research Society (ISSCR) 2015 4. Combination of Alpha-1-Acid Glycoprotein and Alpha-Fetoprotein as an
Improved Diagnostic Tool for Hepatocellular Carcinoma”, Patent Registered in USA and Australia.
RECENT JOURNALS/TEXTBOOK CHAPTER (2016 only) TEXTBOOK 1. INTECH Open Science Textbook Chapter, Hypoxia in Mesenchymal Stem
Cell, Chapter 5, Thomson Reuters, ISBN 978-953-51-2896-0. 2016 ARTICLES 2016 1. Wahyu W, Laura W, Dwi A, Harry M, Nurul F, Sutiman B. S, M. Aris W and
Indra B; Biomarkers and Genomic Medicine, In Press, Available online 9 March 2016
2. Mahanani, Erlina Sih, Bachtiar, Indra, Ana, Ika Dewi; Key Engineering Materials, Vol. 696, p205-211. 7p, 2016
25
ORAL PRESENTATION
26
THE COMPARISON BETWEEN LECTURING AND CADRE RECRUITMENT
METHOD TO INCREASE KNOWLEDGE, BEHAVIOR AND ATTITUDE IN
CEMPAGA VILLAGER ABOUT CATARACT BLINDNESS
AA Mas Putrawati Triningrat, AAA Sukartini Djelantik, Ni Made Ari Suryathi
Ophthalmology Department, Faculty of Medicine, University of Udayana
ABSTRACT
Introduction and Objective: Cataract is the highest cause of blindness in Bali. Cempaga
village which located in Singaraja Bali island is original village that have been surveyed for
cataract blindness previously and the number is almost 50%. According to the survey, the
most barrier of cataract surgery is less of knowledge about cataract. The purpose of this study
is to compare the two methods, lecturing and cadre recruitment to increase knowledge,
behaviour and attitude. Methods: This is cross sectional study, and using questionnaire
before and after intervention of two methods Result: Lecturing method statistically
significant to increase knowledge in respondent (p<0,001). Attitude and behaviour seems not
significant increased. In this study, cadre recruitment method not significant to increased
knowledge, attitude and behaviour. Conclusion: In this study we found only lecturing
method can increase significantly knowledge about cataract blindness. We suggest for a long
term study and a regular training for cadre in population to give a good services in order to
improve knowledge, behaviour, attitude concerning eye health especially cataract blindness.
Keywords: lecturing method, cadre recruitment, knowledge, attitude, behaviour, cataract
blindness
27
PURPLE SWEET POTATO TUBER ETHANOLIC EXTRACT DECREASES VCAM
EXPRESSION IN HIGH-CHOLESTEROL DIET-FED RABBITS
Bagus Komang Satriyasa
Department of Pharmacology and Therapy,
Faculty of Medicine, Udayana University
ABSTRACT
Coronary heart disease (CHD) is commonly caused by atherosclerosis. CHD is one of the
most common death-causing diseases globally, especially in developed countries.
Atherosclerosis can be caused by increased expression of proinflammatory cytokines such as
IL-1, IL-6, IL-8, monocyte chemoattractant, VCAM and ICAM, and also enzymes such as
COX-2 and matrix metalloproteinases (MMPs). The aim of this research was to evaluate the
effect of anthocyanin-containing purple sweet potato tuber ethanolic extract in inhibiting
atherosclerosis in rabbits. This study was experimental laboratory study, with randomized
control group post-test-only design, using 16 local Balinese rabbits (4-5 months-old) obtained
from Animal Unit Laboratory. This study found that mean VCAM expressions are 63.3750
and 6.1250, for control (first) and treatment (second) group, respectively. Statistical analysis
showed significant difference in VCAM expression (p<0.05). Therefore, anthocyanin-
containing purple sweet potato tuber ethanolic extract can inhibit atherosclerosis via the
inhibition of VCAM expression.
Keywords: Purple sweet potato ethanolic extract, VCAM, rabbits
28
ANTIOXIDANT POTENTIAL AND HYPOLIPIDEMIC EFFECTS OF COMBINED
PURPLE SWEET POTATO TUBER EXTRACT WITH HONEY IN RATS GIVEN
HIGH CHOLESTEROL FEED
I W P Sutirta Yasa1, I Made Jawi
2*
1.Department of Clinical Pathology, Faculty of Medicine, Udayana University
2.Department of Pharmacology Faculty of Medicine, Udayana University
Denpasar Bali Indonesia.
Corresponding: E-mail: [email protected]
ABSTRACT
Flavonoids prevents cholesterol rising in blood and has antioxidant effects. The purpose of
this study is to prove hypolipidemic and antioxidant effect of purple sweet potato tuber
extract with natural honey combination, which contains flavonoids. This is a true
experimental study using randomized pre-post-test control group design. With 28 adult male
wistar rats, were divided into 4 groups. Before treatment, lipid profiles, MDA, and SOD were
examined as a pre-test data. During the 4 weeks of treatment, Group 1 were given high-
cholesterol feed. Group 2 were given high-cholesterol feed and water extract of purple sweet
potato tubers 200 mg / day. Group 3 were given high-cholesterol feed and natural honey 3 cc
/ day. Group 4 were given high-cholesterol feed and water extract combination of purple
sweet potato tuber with natural honey. After the treatment, the blood were examined again as
the pre-test data. The obtained data were tested using t-test and ANOVA. The results showed
a significant increase of total cholesterol, LDL, and MDA in blood (p <0.05), and
significantly decrease HDL in group 1 (p <0.05). In group 2, 3, and 4 there were no
significant difference of these parameters (p> 0.05), but significantly difference with group 1
(p <0.05). The conclusion is the combination of water extract of purple sweet potato tuber
with honey, can maintain lipid profile and MDA in the normal range, at rats with high-
cholesterol feed.
Keywords: Purple sweet potato, honey, lipid profile, SOD, rat.
29
ENDOGENOUS ANTIOXIDANTS AMONG PROPORTIONAL EXERCISE
Made Kurnia Widiastuti Giri
Ganesha Educational University
Email: [email protected]
ABSTRACT Exercise has been declared as one of the natural anti-aging therapies. Studies on the anti-
aging effects of exercise did through many expertise,such one of them is through
immunological studies. The purpose of this study was to analyze the differences in the levels
of specific endogenous antioxidant produced by the mitochondria that was Superoxide
Dismutase (SOD). Methods: This study was a randomized control trial on two group, with a
power of 80%, α = 5% used 20 experimental rats were simple randomly divided into two
groups: sedentary and exercise group proportional (moderate exerciseintensity, 10 minutes a
day, 3 times a week). Treatment was given for 6 weeks and at the end of treatment, blood
examination by using Enzyme Linked Immunosorbent Assay (ELISA) on serum of both
groups. Data were analyzed using parametric statistical tests paired sample t test to test the
difference in results between the dependent variable and the initial observation period after
treatment, while the differences between groups is done with the Chi square test. Results :In
comparison with sedentary groups, proportionated exercise resulted increases performance,
reduction in MDA and increases of SOD (performance 0.6± 5.7 vs 5.2 ± 5.9 vs., p < 0.01;
MDA −5.1 ± 5.9 vs. 3.9± 5.5, p < 0.02; SOD 0.7 ± 2.2 vs 5,6± 6,8. =, p = 0.02). Conclusion:
Among rats in proportioned exercise, we found that participation in an 6-week intervention
was feasible and resulted SOD increases in blood. Thus would be recommended for future
research wtih biomolecular technique analyzing exercise proportinally combined with other
antiaging therapies.
Keywords: exercise, anti-aging, SOD
30
MOLECULAR DETECTION OF AVIAN INFLUENZA VIRUS FROM BIRDS SOLD
IN A MULTISPECIES ANIMAL MARKET JAKARTA
Dordia Anindita Rotinsulu1*
, Surachmi Setiyaningsih1, Abdulgani Amri Siregar
1
1Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia
*corresponding author: [email protected]
ABSTRACT Avian Influenza (AI) is a zoonotic disease caused by influenza virus A of the family
Orthomyxoviridae. The Avian Influenza virus infects various birds and mammals, including
human. Multispecies animal market, as a meeting place for human, birds, mammals, and
reptiles can potentially transmit AI virus among animal to human. The purpose of this study
was to detect the presence of Avian Influenza virus (Matrix gene and H5 gene) from various
bird species sold in a multispecies animal market in Jakarta using real-time Reverse
Transcriptase-Polymerase Chain Reaction (rRT-PCR). A number of 862 field samples from
28 families and 96 bird species were collected using cross-sectional sampling technique in a
multispecies animal market, Jakarta. These field samples consisted of 649 fecal samples, 114
cloacal swab samples, and 99 oropharyngeal swab samples. The samples were pooled based
on the type of sample and kiosk where the sample was collected. To detect the presence of AI
virus, molecular technique by using real-time RT-PCR was preformed targeting the matrix
gene of AI virus. Samples which were positive for Matrix gene were further assessed using
real-time RT-PCR targeting the H5 gene for AI virus subtyping. As many as 7 of 649 fecal
samples (1.08%) were positive for Matrix Gene AI Virus. However, none cloacal or
oropharyngeal swab were positive for AI. Positive fecal samples were detected in four bird
species, which are three magpie-robins (Copsychus saularis, family Turdidae), two white-
eyes (Zosterops palpebrosus, family Zostropidae), a yellow-vented bulbul (Pycnonotus
goiavier, family Pycnotidae), and a robin (Leiothrix lutea, family Sylviidae). Based on
molecular detection using real-time RT-PCR, none of the samples were positive for H5 gene
of AI virus.
Keywords: Avian Influenza, birds, matrix gene, real-time RT-PCR.
31
K-RAS MUTATION PROFILE IN COLORECTAL CARCINOMA PATIENTS IN
SANGLAH HOSPITAL
Luh Putu Iin Indrayani M, Ni Putu Sriwidyani
Anatomical Pathology Department, Faculty of Medicine Udayana University/Sanglah
General Hospital
ABSTRACT
Introduction: Today is an era of personalized medicine where treatment is tailored
personalized to each patient status. K-ras mutation is one of molecular examination in
colorectal carcinoma and the result will direct treatment decision to patient. Patient with K-
ras mutation should not to be treated with anti-EGFR. This study was a preliminary study to
determined K-ras mutation profile in colorectal carcinoma in Bali. Methods: Formalin-fixed
paraffin embedded tissue of 23 colorectal carcinoma patients in Sanglah Hospital were
collected in Anatomical Pathology Department and sent to Dr. Cipto Mangunkusumo
Hospital for K-ras examination in the year 2016. All of H-E slides were examined and the
spesimen with maximum of well preserved tumor cells were selected. Mutation of K-ras gene
was performed by PCR. Result: From 23 cases, 70% was male and 30% was female, with
mean age was 48.3 year. Fourteen cases (60.9%) had K-ras mutation (9 cases with codon 12
mutation, 4 cases with codon 13 mutation, 1 case with codon 59 mutation and 1 case with
codon 117 mutation). Conclusion: It is found that K-ras mutation rate was 60.9% and the
two most common mutations observed in codon 12 and 13.
Key words: K-ras, mutation, colorectal cancer, Bali
32
THE EFFECT OF CACAO BEANS EXTRACTS ADMINISTRATION ON SOD AND
OX-LDL CONCENTRATION IN OXIDATIVE STRESS CONDITIONED RAT
Dewi Wiryanthini IA1, Dewi Ratnayanti IGA
2, Sutadarma IWG
1
1Biochemistry Department,
2Histology Department Faculty of medicine Udayana University
Email : [email protected]
ABSTRACT
A study in adult Wistar white rats on the effect of extract of cacao beans on the SOD and ox-
LDL level in oxidative stress induce by psychosocial stress was carried out. As many as 20
adult Wistar white rats with oxidative stress induced by psychosocial stress in this study were
divided into 4 groups with 5 rats in each group in Pretest-Postest Control Group Design as
Control group or placebo (P0), intervention group by 70 mg extracts of cacao beans (P1), 140
mg (P2), and 280 mg (P3) for 14 days. All groups were examined for blood SOD and ox-
LDL concentration before and after intervention. This study revealed increase significantly
with p=0,000 on SOD concentration in oxidative stress white rat induced by psychosocial
stress after administration of cacao beans extract in group P1 P1 (535,59 vs 554,24), P2
(540,68 vs 632,2) dan P3 (545,76 vs 696,61) with not significantly decrease of ox-LDL
(p=0,957). This study showed that cacao beans extract can inhibit oxidative stress caused by
psychosocial stress with increased SOD concentration although not effective decreased ox-
LDL.
Keywords: Psychosocial stress, oxidative stress, cacao beans extract, SOD, ox-LDL
33
THE DIFFERENCE IN MOTOR IMPROVEMENTS RELATED TO COMBINATION
OF OMEGA-3 POLYUNSATURATED FATTY ACID AND ALPHA-
TOCOPHEROLSUPPLEMENTATIONSDIET OFWEIGHT-DROPPED INDUCED
SPINAL CORD INJURY IN RATS
Mahadewa Tjokorda GB, Wisnu A. Wardana, Wisnu Wardhana
Neurosurgery Department Udayana University-Sanglah Hospital, Bali, Indonesia
Address correspondence to:TjokordaGB Mahadewa, MD.Ph.D., Associate Professor of
Neurosurgery, University of Udayana, Division of Neurospine, Sanglah General Hospital,
KesehatanStreet 1, Denpasar, Bali, Indonesia 80000. Phone: (62361) 249988, Fax: (62361)
244322, e-mail: [email protected]; [email protected]
ABSTRACT
Spinal cord injury (SCI) is the cause of severe neurological disability, and no satisfactory
treatment is currently available. Evidence suggests that omega-3polyunsaturated fatty acid
(PUFA) could target some of the pathological mechanisms that underlie damage after SCI. In
parallel, promising effect of alpha-tocopherol(vitamin E) also shown as a potent antioxidant
to prevent lipid peroxidation on SCI. Therefore, we aim to investigate the combination effect
of omega-3 PUFA and vitamin E supplementation in improving motor function of weight-
dropped induced SCI in rats.
Twenty five rats were randomly divided into the five following groups: control group,
omega-3PUFA group, vitamin E group, combination treatment (omega-3 PUFAand vitamin
E) group and sham operated group. SCI was induced in the rats by using a weight-dropped
model around T9-T11 vertebra level. Omega-3PUFA and vitamin E was administered orally
on each day for two weeks of treatment. The sham group underwent laminectomy without
spinal cord compression and treatment. Motor function was measured using Basso, Beattie
and Bresnahan (BBB) locomotor scaleat the 1st, 7
th and 14
th day.We found significant
difference of motoric function between omega-3 PUFAgroup and combination treatment
group compared to the control (p<0,05). The highest BBB score on the first week was found
in combination treatment group (mean 16±2,3) but no significant difference was found
compared to the omega-3 PUFA group. On the subsequent week, combination treatment was
showing significant improvement compared to the omega-3 PUFAgroup (p<0,05).
These finding had demonstrated the effect of omega-3PUFA as neuroprotective agent to
prevent the secondary injury in SCI induced rats. Combination of omega-3 PUFAwith
vitamin E improves its efficacy to become a promising novel therapy for SCI.
Keywords: Omega-3 PUFA, vitamin E, improves motor function.
34
ULTRASOUND EVALUATION OF NEPHRITIS, HEPATIC STEATOSIS AND
KNEE JOINT EFFUSION IN BALINESE WOMEN WITH SYSTEMIC LUPUS
ERITHEMATOSUS
Elysanti Dwi Martadiani1, Andi Manaek Hatimbulan Panjaitan
2, Widyanto Pangarso
Adhi2, I Gde Raka Widiana
2, Dewa Ayu Ratna Dewi
1
1Department of Radiology, Faculty of Medicine, Udayana University, Sanglah Hospital,
Denpasar, Bali, Indonesia 2Department of Internal Medicine, Faculty of Medicine,Udayana University, Sanglah
Hospital,
Denpasar, Bali, Indonesia
ABSTRACT Introduction: Organ involvement such as nephritis, hepatic steatosis and knee joint effusion
may influence the severity of systemic lupus erithematosus (SLE). Ultrasound (US) can
detect morphological changes of those organs in SLE patients, but there is lack of data about
US-detected lupus organs involvement in Balinese women. Objective of this study is to
investigate the US findings of nephritis, hepatic steatosis and knee joint effusion in Balinese
women with SLE. Method: This study was an analytical cross sectional study. Inclusion
criteria were Balinese women, age more than 12 years, who had been diagnosed as SLE.
Exclusion criteria were heavy-drinking of alcohol, end-stage renal disease, acute knee
infection, and during US evaluation reveals hydronephrosis, renal abscess, emphysematous
pyelonephritis, kidney stone, or kidney tumor. Anamnesis, physical, laboratory and US
examination were done. The prevalence of US-detected nephritis, hepatic steatosis, and knee
joint effusion was determined. Association between several clinical and laboratory results
with US findings were tested using Spearman’s correlation and binary logistic regression
tests (α = 0.05). Result: From 57 subjects, US demonstrated nephritis in 19 (33%) subjects,
hepatic steatosis in 31 (54.5%) subjects, right knee joint effusion in 12 (21.1%) subjects and
left knee joint effusion in 14 (24.6%) subjects. No significant correlation between
dyslipidemia and hepatic steatosis (p>0.05), but there were significant correlations between
knee pain and knee joint effusion (p<0.001), also between decreased of renal function with
right and left nephritis (p<0.05). After adjustment with several laboratory indicators, only
right nephritis had significant association with decreased of renal function (p<0.05).
Conclusion: Nephritis, hepatic steatosis and knee joint effusion were identified in Balinese
women SLE patients using US. Some clinical and laboratory indicators had significant
correlation with knee joint effusion and nephritis, but most of laboratory parameters
demonstrated no significant correlation with US-detected nephritis and hepatic steatosis.
Keyword: systemic lupus erythematosus, nephritis, hepatic steatosis, knee joint effusion,
ultrasound
35
HEPCIDIN AND MATRIPTASE-2 BECOME POTENTIAL BIOMARKER FOR
RESPONSIVENESS TO ORAL IRON SUPPLEMENTATION IN FEMALE
ADOLESCENTS WITH IRON DEFICIENCY ANEMIA
Yuliana Heri Suselo, Sri Wulandari, Amelya Augusthina Ayusari, Dono Indarto
Research Group of Human Proteomics, Faculty of Medicine,
Universitas Sebelas Maret Surakarta
ABSTRACT Background. Iron deficiency anemia (IDA) is one of nutritional problems for all ages in
Indonesia. Iron fortification and supplementation has become national program for treatment
of IDA in pregnant women. The prevalence of IDA in female adolescents is also high, but
there is no program for IDA treatment. Higher levels of hepcidin are related to non-
responsiveness to oral iron supplementation. Matriptase-2 is a major enzyme which regulates
hepcidin secretion. Thus, the aim of this study was to investigate whether or not female
adolescents were responsive to oral iron supplementation. Methods. This was a quasi-
experimental study with pre- and post-test group design. Research subjects were187 female
adolescents who studied in year 10 and 11 of secondary schools in Boyolali regency.
Hemoglobin (Hb) levels were measured using the cyanmethemoglobin method and ferritin
serum used ELISA. Female adolescents with IDA were given 1 tablet ferro-fumarate per day
for 1 month. After treatment, Hb levels were determined using the same method and serum
levels of hepcidin and matriptase-2 were detected by ELISA. All data were analyzed using
independent t-test with 95% significance level. Results. Around fifty percent female
adolescents had IDA and 38.2% was responsive to iron supplementation (ΔHb ≥ 1 g/dL)
while 61.8% was non-responsive (ΔHb < 1 g/dL). Hepcidin levels of non-responsive
subjects (6.8 ng/mL) were higher than that of responsive subjects (6.4 ng/mL) but it did not
reach significant difference (p=0.30). Lower levels of matriptase-2 were significantly
observed in non-responsive subjects (666.3 pg/mL), compared with responsive subjects
(1133.0 pg/mL) (p=0.05). Conclusion. More than 50% of female adolescents is not
responsive to oral iron supplementation with higher levels of hepcidin and lower levels of
matriptase-2. Both proteins are a potential biomarker for detection of responsiveness to oral
iron supplementation.
Key words : Hepcidin, IDA, Matriptase-2, Responsiveness, Oral iron supplementation
36
THE DIFFERENCE EXPRESSION OF GLUT4 AND GLYCOGEN LEVELS ON
CELLS OF LIVER AND MUSCLE TISSUES IN HYPERGLYCEMIC AND
NORMOGLYCEMIC CONDITIONS
I Nyoman Suarsana1*
, Iwan Harjono Utama1, I Putu Gde Yudhi Arjentinia
2,
I Made Kardena3, Anak Agung Ayu Mirah Adi
3
1Laboratory of Biochemistry, Faculty of Veterinary Medicine, Udayana University, Jln. P.B.
Sudirman Denpasar, Bali, Indonesia 80233 2Laboratory of Internal Disease Veteriner, Faculty of Veterinary Medicine, Udayana
University, Jln. P.B. Sudirman Denpasar, Bali, Indonesia 80233 3Laboratory of Phatology Veteriner, Faculty of Veterinary Medicine, Udayana University,
Jln. P.B. Sudirman Denpasar, Bali, Indonesia 80233 *Correspondence to: I Nyoman Suarsana,
Laboratory of Biochemistry, Faculty of Veterinary Medicine,
Udayana University, Bali-Indonesia : [email protected]; [email protected]
ABSTRACT
Background: Under normal physiological conditions, the glucose uptake into the cell is
regulated by glucose transporter (GLUT4) which is stimulated by insulin to be afterward
metabolized into energy and or stored into glycogen. The research aimed to determine the
expression of glucose transporter (GLUT4), glycogen levels on cells liver and muscle tissues
in hyperglycemic and normoglycemic conditions on rats. Method: A total of 20 rats of
Spraque Dawlly strain were grouped into 2 treatment groups. The normal group and the
hyperglycemia group. The observations were performed on: blood glucose level by glucose
oxidase biosensor method by using the Blood Glucose Test Meter of GlucoDr, liver and
muscle glycogen levels by spectrophotometry and GLUT4 analysis on cells of liver and
muscles tissues by using immunohistochemical technique. Results: The results showed that
80% of sucrose administration has caused hyperglycaemic rats with the blood glucose levels
of 131.2 g/dl. In hyperglycemic conditions, the levels of liver glycogen (9.4 g/mg) and
muscle (8.68 g/ mg) were significantly higher (P <0.05) than in the liver (8.54 g / mg) and
muscle (7.87 g /mg) in normoglycemic. Meanwhile, the liver glycogen levels were
significantly higher (P <0.05) when compared with the muscle glycogen levels in both
hyperglycemic and normoglycemic conditions. The expression of GLUT4 in hyperglycemic
was significantly higher (P <0.05) in both liver (64.05%) and muscle (56.15%) when
compared with the liver (53.21%) and the muscle (48.67% ) in normoglycemic. Between the
liver and muscle tissues, the expression of GLUT4 was significantly higher (P <0.05) in the
liver when compared with muscle tissue in both hyperglycemic and normoglycemic
conditions. Conclusion: Expression of GLUT4 and levels of glycogen in hyperglycemic liver
is higher compared to the muscles.
Keywords: glucose, glycogen, hyperglycemic, GLUT4
37
COMPARISON OF ANTIOXIDANT POTENTIAL OF ETHANOL EXTRACT WITH
AQUEOUS EXTRACT OF PURPLE SWEET POTATO TUBER IN HUMAN
VASCULAR ENDOTHELIAL CELLS EXPOSED TO H2O2
I Made Jawi1, I G K Arijana2
1.Department of Pharmacology, 2. Department of Histology,
Faculty of Medicine, Udayana University, Jl. PB. Sudirman Denpasar
Bali Indonesia.
*Corresponding author’s email :[email protected]
ABSTRACT Oxidative stress causes endothelial dysfunction. Water extract of purple sweet potato tubers
have antioxidant potential in animal and human vascular endothelial, through increased
superoxide dismutase (SOD). This study aims to prove the comparison between ethanol
extract and aqueous extract of purple sweet potato tubers increasesing the expression of
SOD-2, SOD-3 and eNOS in human vascular endothelium. This study was an experimental
study of the vascular endothelial cells which undergo oxidative stress in vitro, which is
protected by ethanol extract or aqueous extract of purple sweet potato tuber at various
concentrations. Observance of the SOD-2, SOD-3 and eNOS in endothelial cells is done
using the method immunohyschemestry with monoclonal antibodies. The results showed an
increase in SOD-2, SOD-3 and eNOS was significantly (p <0.05) in endothelial protected by
ethanol extract or water extract of purple sweet potato tubers. The ethanol extract only
showed its effect at concentration of 1.5625 µg/ml for SOD-2 and SOD-3. The effect of
aqueous extract were at a concentration of 1.5625 to 3.125µg/ml. From these results it can
be concluded that the ethanol extract or aqueous extract of purple sweet potato tubers can
protect endothelial cells from oxidative stress through increased expression of SOD-2 and
SOD-3 .Ethanol extract requires much lower concentrations to show its effect compared with
aqueous extracts.
Keywords: Aqueous extract or ethanol extract of purple sweet potato, SOD, HUVEC.
38
THE PREVALENCE OF BACTERIAL VAGINOSIS (BV) AMONG
REPRODUCTIVE WOMEN IN BALI
Septarini NW1)
, Sutarga IM1)
, Suariyani LP1)
1)
School of Public Health, Faculty of Medicine, Udayana University, Denpasar, Bali
*Correcponding Author: [email protected]
ABSTRACT Bacterial Vaginosis (BV) is one of the most common vaginal infections among women in
reproductive age. This infection occurs due to the reduction of number of lactobacillus
bacteria and the massive increase of anaerobic bacteria on vagina. Gardnerella vaginalis is
one of the agents of disease. If it is left under-diagnosed, it will course serious complication
to the women, including uterus infection, pelvic inflammatory disease, sterility as well as
outside reproductive organ complication. It is also increase the chance of HIV infection. In
Bali, there was no research on the prevalence and determinants of BV. This was a cross
sectional study conducted in two STIs clinics in Bali. The samples were women in
reproductive age that visited those clinics. Besides collecting vaginal secret from the subjects
to be tested using gram stain methods to find clue cells, the subjects were also asked about
BV’s determinants. Ethical clearance was obtained from Medical Faculty/Sanglah Hospital,
Udayana University. Subjects were given informed consent prior to sample collection. The
total subjects joined this study were 100 women. The prevalence of Bacterial vaginosis
among subjects was 67%. No statistically significant relationship was found between
determinants including age, contraceptive use, parity, and condom use with the increase risk
of getting BV infection. However, the high prevalence of this disease suggest that there is a
need to screen this disease is one of the prevention matter at the general community level in
order to give prompt treatment and prevent the spread and complication.
Keywords : Bacterial vaginosis, determinants, reproductive women
39
LOW PREVALENCE OF CAVEOLIN-1 ONCOGENIC POLYMORPHISM G14713A
AND T29107A AMONG BREAST CANCER PATIENT IN SANGLAH GENERAL
HOSPITAL
Desak Made Wihandani1, Putu Anda Tusta Adiputra
2, I Gede Putu
Supadmanaba1,1Biochemistry Department Faculty of Medicine Udayana University
2Oncology Surgery Division Faculty of Medicine Udayana University/Sanglah General
Hospital
Corresponding Author: Desak Made Wihandani, Biochemistry Department Faculty of
Medicine Udayana University. Email: [email protected]
ABSTRACT
Background: Currently, breast cancer is one of the most common cancers among women,
surpassing only by cervical cancer. Despite the improvement in terms of survivability as well
as prognosis, most patients are coming with advanced disease at the time of diagnosis. Unlike
early disease, determining the outcome of advanced disease is still challenging. Initial
investigation showed that polymorphism within caveolin-1 gene could also contribute in
determining risk as well as prognosis. In our initial study, we investigated the prevalence of
two oncogenic polymorphism of Caveolin-1: SNP CAV1 G14713A and T29107A. Method:
The samples were obtained by consecutive sampling in Sanglah Hospital from January 2016
to December 2016. The blood samples were collected in EDTA tube and the DNA was
isolated using Promega Kyt. Subsequent PCR was conducted to amplify the gene which then
sequenced in Genetika Science. Result: We enrolled 43 samples with complete medical
records in our analysis. The mean age in our sample was 49.11±10.33 years and mostly
stadium III cancer (39.7%) and poorly differentiated. 68% of our sample were luminal types
and the rests were HER2 and TNBC with comparable proportion. We only detect one
heterozygous sample for G14713A polymorphism in which the patient had Luminal A type
but with high Ki67 and poorly differentiated. For T29107A, we detect two heterozygous
individuals and one homozygous individual, all with the same characteristics with G14713A.
Conclusion: The prevalence of SNP CAV1 G14713A and T29107A were considerably low in
breast cancer population in Bali. However, the characteristic of the samples with the
polymorphism suggest that further investigation is needed to confirm their effect in brest
cancer morphology.
Keywords: Breast cancer, Caveolin-1, Polymorphism, G14713A and T29107A.
40
TAENIA SPECIES IDENTIFICATION BY MPCR ON TAENIASIS PATIENT WHO
VISITED PARASITOLOGY DEPARTMENT FACULTY OF MEDICINE UDAYANA
UNIVERSITY FROM 2014 TO 2016
Kadek Swastika, Made Sudarmaja, Luh Ariwati
Department Of Parasitology Faculty of Medicine Udayana University
Corresponding Author: Kadek Swastika; Department Of Parasitology Faculty of Medicine
Udayana University. Email: [email protected]
ABSTRACT
Taeniasis/Cysticercosis is a zoonosis belonging to the neglected diseases. Now is still a health
problem in some developing countries. Taeniasis/cysticercosis is one of health problem in
Indonesia, Bali is one of the three endemic areas beside Papua and Nort Sumatera. Three
human Taenia species which mature in the human intestine are known in Asia. Taenia
solium, T. saginata and T. asiatica. The aim of this study was to indentify the species of
taenia by multiplex PCR and to know the prevalence of the cysticercosis among taeniasis
carriers. The progglotids/segments of the worm from the Taeniasis patient who came to
Department of Parasitology Faculty of Medicine Udayana University from 2014-2016 were
collected. Patients were treated with Yomesan® 2 gr and the proglotids/worm were collected.
These proglotids were Identified by multiplex PCR. From the taeniasis carrier also collect the
serum and confirmed by ELISA the if there were cysticercosis cases. There were total 23
worm/proglotids were collected from 23 taeniasis carrier. Patients came from four regencies
of nine regencies in Bali were : Gianyar, Badung, Denpasar and Karangasem. A total of
69.6% (16/23) patients came from Gianyar regency . There were 69,6 % patients were male
and 30,4% were female. All of proglotids were confirmed as Taenia saginata by multiplex
PCR. There were no cysticersosis cases were confirmed by ELISA among the taeniasis
carriers. The integrated health education and sustainable program to detected and deworming
carriers are important to prevent and control this disease
Keywords : Taeniasis, Bali, Cysticercosis
41
C-1562T POLYMORPHISM OF MATRIX METALLOPROTEINASE-9 (MMP-9)
GENE ASSOCIATED WITH ELEVATED LEVEL OF PLASMA MMP-9
CONCENTRATION IN PATIENT WITH ACUTE MYOCARDIAL INFRACTION
(AMI) IN DENPASAR, BALI
1Tianing Ni Wayan.,
2Bagus Ari Pradnyana Dwi.P., 1D.M Wihandani
1Department of Biochemistry Faculty of Medicine, Udayana University, Bali-Indonesia
2Faculty of Medicine Udayana-University/Sanglah Hospital, Bali Indonesia
ABSTRACT
Background: Acute Myocardial Infarction (AMI) is emergency medical condition which
still has high mortality and morbidity. In recent decades, it prevalence has a tendency to
increase, parallel with other chronic diseases. One of suspected contributing factor in AMI is
the presence of C1562T polymorphism on MMP-9 gene which increase plasma MMP-9
concentration and destabilize the plaque. However, this notion needs further confirmatory
studies, as there are several contradictive reports regarding their association. This Study
aimed to determine the relationship between C1562T polymorphism with the increase of
MMP-9 concentration in AMI. Method: A cross-sectional was conducted in Cardiovascular
Centre of Sanglah General Hospital. The peripheral blood samples were obtained from
subjects and the DNA and blood plasma was isolated. The polymorphism was detected using
PCR and RFLP while MMP-9 concentration was determined by ELISA. Results: The
average concentration of plasma MMP-9 was found to be at 8.33 ng/mL which ranged from
0.74 ng/mL until 31.93ng/mL. The proportion of MMP-9 C1562T polymorphism was 20%
from all subjects. The average concentration of MMP-9 was higher than cut-off standard
which is 0.6 ng/mL. Analysis within the group revealed that CT-genotype had significantly
higher average MMP-9 compared with CC phenotype (11.27 ng/mL vs 7.65 ng/mL).
Conclusion: C1562T polymorphism appears to be significantly associated with AMI by
increasing the concentration of MMP-9 in blood plasma.
Keywords: C1562T Polymorphism, MMP-9, Acute Myocardial Infarction
42
DETECTION OF THEA3243G MTDNA MUTATION BY PCR ALLELE SPECIFIC
AMPLIFICATION (PASA) FOR DIABETES MELLITUS TYPE 2 AT SANGLAH
HOSPITAL BALI
Surudarma W, Wihandani DM
Biochemistry Department, Medical Faculty of Udayana University
Jl. PB. Sudirman, Denpasar, Bali, Indonesia
ABSTRACT
Genetic factors play a role as a cause of type 2 diabetes mellitus. Genes in mitochondrial
DNA (mtDNA) can be the cause of type 2 diabetes mellitus because oxidative
phosphorylation that produce ATP in mitochondria plays an important role in insulin
secretion by pancreatic β cells. The dysfunctionof insulin secretion is associated with the
mutation of A to G at the 3243th nucleotide position of the tRNAleu gene of mitochondrial
DNA.This mutation has been shown as a causal mutation in maternal-derived diabetes
mellitus accompanied by deafness, Maternally Inheridited Diabetes and Deafness (MIDD).
Characteristics of mutations A3243G mtDNA is a heteroplasmic mutation with a relatively
low number of mutant DNA.Several studies in Indonesia have reported the difficulty of
detecting such low frequency mtDNA heteroplasmic mutations. Therefore a precise, accurate
and relatively inexpensive method is needed. The method chosen in this study was PCR
Allele Specific Amplification (PASA)that performed on two tubes using 3 primary types,
universal primer 5'-AAC GTT GGG GCC TTT GCG TA-3 '(D1),normal primer 5'-GGG TTT
GTT AAG ATG GCA GA-3' (DN), and mutant primer 5'- GGG TTT GTT AAG ATG GCA
GG-3' (DMt). Samples that containing A3243G mtDNA mutations will produce a PCR
product with a 200 pb band on both tubesbecause heteroplasmicmutation contain of mutant
mtDNA and normal mtDNA.In this study found heteroplasmic mutation A3243G mtDNA in
21 samples of 96 patients with type 2 diabetes mellitus.
Keyword: mutation, diabetes mellitus, PASA, heteroplasmic.
43
Potential Role Of Snakes In Salmonella enteritidis And Arizona
hinshawii Transmission To Human: Zoonosis
Silvi Dwi Anggraini1 and Yos Adi Prakoso
2,*)
1Asa Animals Clinic, Kediri, Indonesia, 64117.
2Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, Indonesia, 55281.
*)author e-mail: [email protected]
ABSTRACT
Salmonella enteritidis and Arizona hinshawii are common bacteria in reptiles. The bacteria
has a potency of zoonosis by human-reptiles contact, especially snakes. The aim of this study
is to isolate S. enteritidis and A. hinshawii from domesticated snakes. During 1 months of this
study a total of 37 cloacal swabs of snakes (Python reticulatus) were collected from reptiles
owner in Surabaya. The cloacal swabs were streaked on MacConkey Agar (MCA), Blood
Agar (BA) and Xylose Lysine Deoxycholate Agar (XLD) and subsequently incubated at 37o
C for 24 hours for primary isolation. Bacterial identification used an endoenzyme test
(catalase and oxidase) and Micro-ID test (Voges Proskauer, nitrate broth, phenylalanine
diaminase, H2S producing, indole, decarboxylase ornithine, decarboxylase lysine, malonate,
urease, esculin, ONPG, arabinose, adonitol, inositol, and sorbitol. The result showed that
Salmonella enteritidis (29,72%) and Arizona hinshawii (40,45%) were isolated and identified
from the collected samples. Current study supports the finding that snakes can be regarded as
critical source of S. enteritidis and A. hinshawii human infection.
Keywords: snakes, S. enteritidis, A. hinshawii, zoonosis
44
VARIOUS SYMPTOM OF CITRUS VEIN PHLOEM DEGENERATION DISEASE
(HUANGLONGBING) CAUSED BY DIFFERENT NUTRIENT DEFICIENCY AS
THE EFFECT OF Liberobacter asiaticum INFECTION
Sheila Simanjuntak, I Gede Putu Wirawan*, and Ni Made Sritamin
Laboratory of Genetic Resources and Molecular Biology
Udayana University
*Corresponding author , E-mail: [email protected] and [email protected]
ABSTRACT Citrus vein phloem degeneration (CVPD) disease or Huanglongbing caused by a Gram
negative bacteria, L. asiaticum is the main disease of citrus plant. The symptom of the
disease very similar to the virus infection and that caused in the beginning was said as virus
infection. Recently, in 1996, it was confirmed that the disease was due to bacterial infection.
This study was done to confirm the nutrient deficiency of the citrus plants was caused by L.
asiaticum infection. Different nutrient deficiency showed different symptom of the disease
and all of those phenomena were caused by different transport protein that affect by the
infection of L. asiaticum.
Keywords: Citrus vein phloem degeneration (CVPD), L. asiaticum, deficiency.
45
LEAD DETECTION IN BLOOD OF THE BALI CATTLE THAT WERE
SLAUGHTERED IN THE TRADITIONAL SLAUGHTERHOUSES IN
DENPASAR
I Ketut Berata
1, I Made Kardena
1, Ni Nyoman Werdi Susari
2,
and I Wayan Sudira3
1Pathology Laboratory of Veterinary Medicine Faculty of Udayana University
2Anatomy Laboratory of Veterinary Medicine Faculty of Udayana University
3Farmacology & Toxicology Lab of Veterinary Medicine Faculty of Udayana University
Jl.PB Sudirman Denpasar – Bali – Indonesia
Corresponding author: [email protected]
ABSTRACT
Contamination of lead in the body of the cattle were thought initially to be coming from the
feed and the environment in which the cattle was maintained. When a human consumes more
than 2.0 ppm of lead-contaminated beef, it could cause damage to various organs, and also
lower cognitive abilities. This study aims to determine the amount of heavy metal lead in the
blood of bali cattle which were slaughtered in a traditional slaughterhouse in Denpasar. Blood
plasma samples of 20 bali cattle were taken randomly and used for this study. The blood
stored in 10ml tubes which were filled with 0.5% Ethylene diamine tetraacetic acid (EDTA)
previously. Lead content measurement were done by using atomic absorption
spectrophotometer (AAS) method. From the measurement result, it was obtained that the lead
content average was 7.35±4.33 ppm. There was a blood sample that did not contain any lead.
The lead content of this result is higher than in the cattle at Suwung Landfill Denpasar, which
was 6.60±1.85 ppm, as the result of our research previously. This results shows that lead
contamination is not only originated from the place in which the cattle was maintained. There
were other risk factors that can contribute to it, which needs to be determined. It can be
concluded that lead contamination in blood plasma of bali cattle which were slaughtered in a
traditional slaughterhouse is 7.35±4.33 ppm. There is the need to do a further study about the
risk factors of lead contamination in the blood of bali cattle.
Keywords: lead, bali cattle, blood plasma.
46
WHOLE PLANT REGENERATION OF BALINESE RED RICE (ORYZA SATIVA
CV. BARAK CENANA) FROM MATURE EMBRYO-DERIVED CALLUS.
Ida Bagus Made Artadana1*
, Gilang Bintang Fajar Suhono1, Popy Hartatie Hardjo
1,
Maria Goretti Marianti Purwanto1, Yoko Brigitte Wang
1
1Faculty of Biotechnology, University of Surabaya, Raya Kalirungkut, 60291, Surabaya,
Indonesia. Tel.: +62-31-2981399. Fax: +61-31-2981278. *corresponding author. Email: [email protected].
Abstract
An efficient regeneration is considered to be important in rice characteristic improvement
through soma clonal mutation or transformation. In this research, we aimed to optimize the
whole plant regeneration step of Oryza sativa Cv. Barak Cenana, a high value local Balinese
red rice, from previously proliferated callus.
Mature embryo-derived callus of Barak Cenana was developed into whole plants by indirect
organogenesis where the roots were firstly regenerated and then followed by shoots
formation. The optimization of Barak Cenana callus regeneration was conducted by culturing
in Murashige and Skoog (MS) basic media, supplemented with various combination of plant
growth regulators. We observed that the fastest green spot formation was resulted in MS
media containing Benzyl Adeno Purine (BAP), a cytokinine.
The highest number of regenerated plants was obtained from MS media + 5 mg/L BAP (i.e.
41 plants from 30 callus) among other concentrations of BAP used (1, 3, 5 and 6 mg/L). In
addition, we also found one albino plant derived from callus in MS media + BAP 1 mg/L
during regeneration. This character was persistent even after three times subcultures, shown
that a soma clonal mutation has been occurred.
In conclusion, we found that whole plant regeneration of Barak Cenana could be achieved by
culturing mature embryo-derived callus in MS media containing 5 mg/L BAP. Moreover, the
improvement of Barak Cenana characteristics may potentially be achieved through soma
clonal variation.
Keywords: Barak Cenana, Callus, Regeneration, BAP
47
THE POTENTIAL OF IKAN BUDU AS A LACTID ACID BACTERIA SOURCE
PRODUCING γ-AMINOBUTYRIC ACID (GABA) TO INDUCED HEAT STRESS IN
BROILER
Lili Anggraini1)
, Yetti Marlida2)
, Wizna2)
, Jamsari3)
dan Mirzah2)
1) Graduate Program Andalas University
2) Department of Nutrition and Feed Technology, Faculty of Animal Science, Andalas University
3) Department of Plant Breeding, Faculty of Agriculture, Andalas University
ABSTRACT
Ikan budu is a traditional food product made of marine fish fermented with the addition of
salt. Lactic acid bacteria (LAB) is a type of bacteria that has a critical role in the fermentation
process. The purpose of this study is to get LAB isolates that have the ability to produce γ-
Amino Butyric Acid (GABA). The study was conducted in several stages, the first was the
isolation of LAB from ikan budu based on the establishment of clear zones on MRSA media
added 2% CaCO3. Second, screening of LAB that has the capability to produce GABA by
using MRB media added 50 mM L-glutame as inducer. Third, the morphological
characterization of isolates. The result of the research showed that 9 of 17 isolates were able
to produce GABA detected through testing with TLC (Thin Layer Chromatography) use the
standards of GABA and iluen consisting of n-Butanol: acetic acid: H2O (v/v/v). Nine isolates
were subsequently named B1, B2, B3, B4, B5, B6, B7, B8 and B9. Quantitative measurement
of GABA production used a spectrophotometer with a color reagent ninhydrin and
absorbance was measured at 570 nm, with the highest yield in isolate B2 (15.365 mg / ml)
and the lowest in isolate B1 (3.765 mg / ml). The observation of morphological and
biochemical activity isolate B2 obtained that the isolates belonged to the gram-positive
bacteria, basil cell and aerobic. Based on catalase and oxidase test that showed negative
results, the isolate B2 can be classified based on the type of Lactobacillus sp. From the results
of this study concluded that LAB sourced from ikan budu has potential as a producer of
GABA which can be used as a feed additive anti heat stress for livestock, where the highest
result found in isolate B2 with production of 15.365 mg / ml.
Keywords: : LAB, GABA, ikan budu, heat stress
48
MEDICAL WASTE MANAGEMENT STRATEGY IN INPATIENT PRIMARY
HEALTH CENTER WITH SYSTEM APPROACH: A SWOT ANALYSIS
I Gusti Ngurah Gede Putra
I Gusti Ngurah Gede Putra1*
, Sang Putu Kaler Surata2, Gusti Ayu Ari Agung
2
1Master Student of Regional Planning and Environmental Management Post Graduate
University Mahasaraswati Denpasar, Indonesia 2Staf Lecturer Science Program of Regional Planning and Environmental Management Post
Graduate University Mahasaraswati Denpasar, Indonesia *Author’s email: [email protected]
ABSTRACT Introduction: Primary health care center with inpatient services should have a proper
management of medical waste. However, this program has not been implemented properly by
any inpatient health care centers and, thus, an analysis of waste management starting from the
input, process, and output is necessary. The purpose of this study was to evaluate and analyze
the strategies of medical waste management in health care centers primarily which have an
inpatient service. Methods: A descriptive, explanatory research was conducted by using
respondents from all parties who directly involved in the management of medical waste.
Observation, questionnaires, and interviews were used to obtain the data and analysis
conducted by focus group discussions using Strength Weakness Opportunity Threats
(SWOT). Results: The Strength we found in this study include implemented medical waste
management in health care centers, sufficient funds, and adequate infrastructures. Identified
weaknesses including untrained personnel, poor planning, and poor discipline in each unit.
Opportunities that identified in our study include written regulations on an environmental
health program, the institution shaped in Public Service Board, regular training programs by
health authorities and the third parties that were available to cooperate in managing medical
waste. Several threats were identified namely environmental pollution, high demands of the
local community and the bottleneck problem in the incinerator operating licenses.
Conclusion: It is possible to implement medical waste management primary health care
center especially which have inpatient services. However, proper training, good planning,
regulation and supervision are required to ensure it continuation and maintaining the quality.
Keywords: medical waste, management, system, inpatient health care center
49
TRANSOVARIAL TRANSMISSION DENGUE VIRUS AND LARVA
DENSITY IN BALI
Sang Gede Purnama
1, Pasek Kardiwinata
1, Tri Baskoro
2
Email : [email protected]
1. Public health department, Medicine Faculty, Udayana University
2. Parasitology department, Medicine Faculty, Gajah Mada University
ABSTRACT
Dengue hemorrhagic fever (DHF) is still a public health problem in Indonesia and it endemic in
most area of Indonesia. Previous studies were only collected the data about density of larvae
whereas rarely studies have been done on the virus virulence. This data is very important to
know the presence and types of dengue virus. Usually virus was identified by PCR analysis
which was highly cost and that caused very rare identification. Imunocytochemistry method is
cheaper than RT-PCR and also could prove their transovarian transmission from mosquito to
eggs. This study was an observational analytic with cross sectional design. Random sampling
technique was used to select 600 respondents from three regencies in Bali including 200
households in Tabanan, 200 households in Gianyar and 200 households in Denpasar. Mosquito
density was measured through a survey of entomology and Maya Index. Aedes aegypti eggs
from ovitrap was installed in the field. Mosquitoes were cultured to identify dengue virus with
immunocytochemistry methods, imunoperoksidase streptavidin-biotin complex (IISBC) on the
head squash and confirmed by RT-PCR.
Results of larva density named house index (HI), container index (CI) and breteau index (BI) in
Denpasar (11.5%; 2.6%; 13.5%), Gianyar Regency (57.5%, 10.9%; 61%), and Tabanan Regency
(33.1%; 10.4%; 42%). This results showed the evidence of transovarial transmission of dengue
virus in eggs. Transovarial index in Denpasar was 11.11%; Gianyar regency was 7.14% and
Tabanan regency was 7.14%. The positive dengue virus serotypes founded were Den-2.
There is evidence transovarial transmission of dengue virus serotypes Den-2. The density of
mosquitoes in areas outside Denpasar were high but the level of virulence of the virus in
Denpasar was the highest. It could became a determinant factor that increases the number of
dengue cases.
Keywords: transovarial, dengue, Bali
50
THE POTENTIAL OF TAPE SINGKONG AS LACTIC ACID BACTERIA PRODUCING
GLUTAMATE TO INCREASE QUALITY OF CARCASS BROILER
Vabera Maslami1)
, Yetti Marlida2)
, Mirnawati2)
, Jamsari3)
and YuliatyShafan Nur2)
1) Undergraduate Program,Andalas University
2) Department of Animal Nutrition and Feed Technology, Faculty of Animal science, Andalas University
3) Department of Plant Breeding, Faculty of Agriculture, Andalas University
ABSTRACT Glutamate acid is the amino acid building blocks of other amino acids, neurotransmitters and
forming the image of taste. Lactic Acid Bacteria (LAB) is one of the glutamate acid-producing
microorganisms are widely found in fermented products. Tape singkong is one of the traditional
foods produced from the fermentation of cassava with the activity of several types of
microorganisms. The study consisted of three stages. Stage 1; isolation LAB Tape singkong from
Padang and Bukit Tinggi . Stage 2; Qualitative and quantitative selection of LAB which
producing glutamate acid. Stage 3; Characterization the best LAB in producing acid glutamate.
The results was obtained 68 isolates of LAB from Padang and 54 Isolates of LAB from Bukit
Tinggi were then selected qualitatively using TLC (then layer chromatographic) were immersed
in a solvent mixture of n-buthanol, acetic acid and water (4: 1: 1 v / v) that 60 isolates of LAB
from Padang and 26 isolates of LAB from Bukit Tinggi producing glutamate acid. After the
selection the performed qualitative quantitative selection with spectrophotometer was measured
at 570nm. Highest Glutamate acid production is isolates from Padang TS.P 15 22.58 mg / ml
and isolates from Bukit Tinggi TS.B 16 16.97 mg/ml. the Two isolates which producing the
most high Glutamate acid Characterization according the organisms by reference to Bergey’s
manual of systemic bacteriology. The characterization of two isolates (TS.P 15 and TS.B 15)
was bacill, convex surface, white milk, and was a gram positive bacteria and aerobic. Based on
catalase test and oxidase test showed that isolate TS.P 15 and TS.B 15 was negative catalase and
oxidase, but for the glucose, sucrose and mannitol test the two isolates were positives and
negatives to lactose test. Based on the characterization, the two isolates were Lactobacillus sp.
The conclusion from this study is there isolates Isolates of LAB TS.P16 (Lactobacillus. Sp)
which potential to produce glutamate acid with the production of 22.58 mg / ml.
Keywords: Glutamate acid, LAB, Tape Singkong
51
POSTER PRESENTATION
52
ANALYSIS OF FOOD USAGE AND NUTRITIONAL STATUS IN RURAL AREA IN
BALI PROVINCE, INDONESIA
Kadek Tresna Adhi1, a)
, Ni Wayan Arya Utami1, b)
, I Made Suka Adnyana1, c)
, Ni Wayan
Septarini1, d)
, Ni Putu Ayu Wulan Noviyanti1, e)
1Fakultas Kedokteran, Universitas Udayana
a)
ABSTRAK
Report of Food and Nutritional Awareness System in 2012 revealed that the condition of food
usage in Bali Province of Indonesia was classified as vulnerable especially in the poor area. This
research aimed to understand the detail condition of food usage and nutritional status in the poor
area of Bali Province. The design of this research was a descriptive cross-sectional observational
study with the qualitative and quantitative approach. The sample of quantitative research was
targeted family. Meanwhile, qualitative research evaluates the opinion from health care
providers, community leaders, and cadres from selected village in Karangasem, Klungkung,
Bangli, and Buleleng district. The data was analyzed descriptively to obtain the proportion of
food usage and nutritional status. The result revealed that there was 75.5% family with
vulnerable food status (VFS), 11.1% with prone food status (PFS) and 1.4% with deficient food
status (DFS). Furthermore, there were 16.4%. To conclude, the region from which the samples
were obtained was in the state of food vulnerability which resulted in the emergence of various
nutritional problem among the children.The active role of government and local community are
needed to optimize the utilization of local food sources to improve the nutritional status of the
local community especially toddlers and children.
Keywords: food utilization, nutritional status, food vulnerability, toddler, and poor area
53
THE RISK OF CERVICAL ADENOCARCINOMA IN PATIENTS INFECTED
WITH HUMAN PAPILLOMAVIRUS TYPE-18
I Gusti Ayu Sri Mahendra Dewi
Department of Pathology Anatomy
Faculty of Medicine Udayana University/ Sanglah Hospital Denpasar Bali
ABSTRACT
Introduction: cancer is still a health problem worldwide. Cervical cancer is the second most
common cancer in women in developing countries. High-risk Human Papillomavirus (HPV) is
well recognized as the causative agent of cervical cancer. Squamous cell carcinoma and
adenocarcinoma are the most and the second most common histopathological type. HPV
genotypes probably correlate to histopathological type. This study aim to prove that infection by
HPV-18 give a greater risk for the occurrence of cervical adenocarcinoma compared with
infection by other high risk HPV types. Methods: This study was case control study.
Adenocarcinoma with HPV-18 positive as the case group and adenocarcinoma positive with
other high risk HPV types as the control group. Histopathological diagnosis performed on H & E
stain. Genotyping of HPV used SPF-10 and specific E7-primer by LiPA. Bivariate analysis was
employed to calculate odds ratios (ORs) and Pearson’s X2 test was applied. Result: Total
number of adenocarcinoma observed in this study were 42 patients, consisted of 12 cases and 30
controls. The most prevalence adenocarcinoma occurred in the age group 41-50 years. The risk
of adenocarcinoma in patients with HPV-18 infection was 2.88 fold compared with infection by
other high risk HPV types (95% CI = 1.12-7.38; p = 0.024). Conclusion: HPV-18 infection
caused cervical adenocarcinoma is 3.0 more than other high risk HPV types
Keywords: cervical adenocarcinoma, HPV-18, histopathological type
54
THE PROFILE OF EPIDERMAL GROWTH FACTOR RECEPTOR MUTATION
DETECTED FROM CYTOLOGIC SLIDES OF LUNG ADENO CARCINOMA
PATIENTS IN SANGLAH HOSPITAL, BALI
Ni Wayan Winarti, Herman Saputra
Department of Pathology Anatomy, Faculty of Medicine,
Udayana University/Sanglah General Hospital, Bali, Indonesia
ABSTRACT
Introduction: Epidermal Growth Factor Receptor (EGFR), KRAS and ALK are prototypical
driver mutation which play fundamental roles in lung tumorigenesis. Improper activation of
EGFR due to somatic EGFR gene mutation, may result in increased cell survival, proliferation,
invasion and metastasis in lung adeno carcinoma. Here in we determined the profile of EGFR
mutation in lung adeno carcinoma using cytologic slides, that may serve useful information in
the management of lung cancer by targeting approach of anti EGFR. Methods: This is a cross
sectional study included all lung adeno carcinoma cases in Sanglah Hospital, Denpasar, who
underwent EGFR mutation examination during the year 2013-2016. The diagnosis and EGFR
mutation examination both using cytologic slides. The frequency and types of EGFR mutation
were collected and compared between age and sex. The result then showed descriptively and
analyzed with chi square test. Result: One hundred and nine cases were included in this study,
consisted of 56% (61 cases) male and 44% (48 cases) female, ranging from 32 until 91 year old.
The peak ages were 61-70 year old (30.3%) and 51-60 year old (29.4%). EGFR examination
revealed 51.4% (56 cases) showed mutation and 48.6% (53 cases) were wild type. Three most
common types of mutation with highest sensitivity to anti EGFR were obtained in this study.
They were Ins/Del exon 19 (25.7%), point mutation of Exon 21 L858R (16.5%) and Exon 21
L861Q (5.5%). The remainder, mutation of Exon 20 T790M (0.9%) and mixed mutation (2.8%),
were considered resistent to anti EGFR. The mutation was more frequent in female (66.7%) than
male (39.3%), but not significanly different between age ≤ 50 (54.8%) and > 50 year old (50%).
Conclusion: In order to improve the management of patients with lung adeno carcinoma, the
detection of EGFR mutation from cytologic slide is valuable.
Keywords: lung adeno carcinoma, cytology, EGFR mutation
55
COMBINATION OF HIGHER DOSE METFORMIN AND LOWER DOSE CISPLATIN
INCREASES APOPTOSIS IN CERVICAL CANCER CELLS LINE
Ratih Dewi Yudhani1, Muthmainah
2, Dono Indarto
3
1 Departement of Pharmacology, Medical Faculty, Universitas Sebelas Maret, Surakarta,
Indonesia 2
Departement of Anatomy, Medical Faculty, Universitas Sebelas Maret, Surakarta,
Indonesia 3
Departementof Physiology, Medical Faculty, Universitas Sebelas Maret, Surakarta,
Indonesia
ABSTRACT
Introduction:Cervical cancer is one of the highest gynecologycal malignancy worldwide
including Indonesia which leads to high morbidity and mortality rate. Cisplatin is the first line
chemotherapy for treatment of cervical cancer but its use is limited because of serious side
effects. Our previous study has also showed that metformin enhances the anti-proliferative effect
of cisplatin in cervical cancer cell line (HeLa). This study aimed to investigate whether or not
HeLa cells treated with metformin and cisplatin enhances cell apoptosis. Methods:2 x 105
HeLa
cells were cultured with RPMI medium supplemented with 10% fetal bovine serum, 2%
penicillin-streptomycin and 0.5% fungizone for 24 hours. HeLa cells were then treated with 60
mM metformin, 40 µM cisplatin or combination of 30 mM metformin and 6.25 µM cisplatin or
7.5 mM metformin and 12.5 µM cisplatin for 48 hours. Apoptotic and necrotic cells were
measured using flow cytometer. All data were statistically analyzed using Kruskall-Wallis
testand the significant value was set up < 0.05. Results:HeLa cells which were treated with 30
mM metformin and 6.25 µM cisplatin had higher apoptosis (10.26 ± 7.72 %) than HeLa cells
without treatment (2.72 ± 2.30 %) but it was not significantly difference (p = 0.26). Mean while,
7.5 mM metformin and 12.5 µM cisplatin administered to HeLa cells decreased apoptosis (4.73
± 1.32%), compared with apoptosis in the earlier combined dose. Unfortunately, apoptosis
induction of these drug combination was much lower than HeLa cells treated with 60 mM
metformin (30.32 ± 22.54%) or 40 µM cisplatin (14.80 ± 3.61%) alone. However, the different
percentage of apoptosis did not reach significance (p = 0.26). Conclusion: Administration of 30
mM metformin and 6.25 µM cisplatin in HeLa cells induces higher apoptosis than that of 7.5
mM metformin and 12.5 µM cisplatin.
Keywords: Metformin, Cisplatin, Apoptosis, HeLa cells
56
S-GENE MUTATIONS OF HEPATITIS B VIRUS IN HEPATITIS B CHRONIC
PATIENTS AFTER 12 WEEKS OF TELBIVUDINE TREATMENT
Yenti Purnamasari1, Susan Irawaty Ie
2, Luthfi Parewangi
3, Nasrum Massi
4, David H.Muljono
2,4,5
1Medicine Faculty of Halu Oleo University, Kendari, Indonesia; 2Eijkman Institute for Molecular Biology, Jakarta, Indonesia;
3Gastrohepatology Department of WahidinSudirohusodo Hospital, Makassar, Indonesia;
4Medicine Faculty of Hasanuddin University, Makassar, Indonesia;
5Sydney Medical School, University of Sydney, Sydney, Australia
Email: [email protected]
ABSTRACT Background: Hepatitis B is an inflammation of liver cells caused by hepatitis B virus (HBV). In
Indonesia, the average prevalence of HBV surface antigen (HBsAg) is 9.4%, categorizing
Indonesia as an endemic area. Telbivudine antiviral therapy aims to suppress HBV replication
targeted on polymerase region, unless in the presence of drug-resistant mutations, the mutant will
replicate continuously. On the other hand, the overlapping structure of the HBV genome means
that alterations in the P gene will affect S gene as well. This study aimed to observe the
mutations in HBsAg and predict the antigenicity index changes of HBsAg mutants in chronic
hepatitis B patients after 12 weeks of Telbivudine treatment. Methods: Serum samples of 26
chronic hepatitis B subjects who have received 12 weeks of Telbivudine treatment in
Gastrohepatology Department of Wahidin Sudirohusodo Public Hospital were collected as
samples of this study. HBV DNA was extracted and amplified with nested polymerase chain
reaction (PCR) method. Positive amplification results were purified, sequenced and analyzed for
the presence of mutations. Result: Overall, there were 21 point mutations were found in HBsAg
from 13 positive samples, one of which is associated with diagnostic failure and escape mutants
of active vaccination, while 2 point mutations are associated with HBIg passive vaccination
escape mutants. Seven mutations found in the ‘a’ determinant of HBsAg, with 4 point mutations
occured within the 1st loop of 'a' determinant showing the highest antigenicity index changes,
which may cause alterations of the immunological properties of the HBsAg mutants.
Keywords: Hepatitis B Virus, S Gene, chronic hepatitis B, telbivudine treatment, week 12
57
ETHANOLIC EXTRACT OF THE POWDER OF RED EARTHWORM (Lumbricus
rubellus) OBTAINED FROM SEVERAL ORGANIC FARMLANDS IN BALI,
INDONESIA: ANALYSIS OF TOTAL PHENOLIC CONTENT AND ANTIOXIDANT
CAPACITY
Ni Wayan Sucindra Dewi,1 Agung Nova Mahendra,
1,2 Gustu Widi Kencana Putra,
3
I Made Jawi,1 Dewa Made Sukrama,
4 Ni Luh Kartini
5
Department of Pharmacology and Therapy,1 Division of Drug Development and Laboratory
Animal, Integrated Biomedical Laboratory Unit, Faculty of Medicine,2 Laboratory of Food
Analysis, Faculty of Agricultural Technology,3 Department of Microbiology, Faculty of
Medicine,4 Faculty of Agriculture,
5 Udayana University, Bali, Indonesia.
ABSTRACT
Background: Red earthworm (Lumbricus rubellus) has been used for centuries as an ingredient
of Ayurvedic therapy and traditional Chinese medicine (TCM). The worm is used in the
treatment of inflammation, hematological disorders, fever, hepatic disorders, joint pain, high
blood pressure, and several other indications. L. rubellus is also bred and developed into powder
in Bali, Indonesia to be used empirically in the treatment of diabetes, stroke, and joint pain.
These disorders are known to be associated with inflammation and oxidative stress. Phenolics are
known to act as anti-inflammatory agent. To our knowledge, there is no data regarding the total
phenolic content and antioxidant capacity of L. rubellus obtained from Bali. Methods: Ethanolic
extract of L. rubellus powder was made in The Laboratory of Division of Drug Development and
Laboratory Animal, Integrated Biomedical Unit, Faculty of Medicine, Udayana University. Total
phenolic content and antioxidant capacity were investigated in The Laboratory of Food Analysis,
Udayana University. Total phenolic content was determined using Folin-Ciocalteu method,
meanwhile antioxidant capacity was determined using DPPH method. Results: We found that
the ethanolic extract of L. rubellus powder had total phenolic content of 1016.31 mg/100 g gallic
acid equivalent (GAE) and exhibited IC 50% of 12.33 mg/mL. Conclusion: We conclude that
ethanolic extract of L. rubellus powder obtained from several organic farmlands in Bali contains
phenolic acid and shows antioxidant effect in vitro. L. rubellus powder can be potentially used as
a natural antioxidant source to treat disorders associated with inflammation and oxidative stress.
Keywords: Lumbricus rubellus powder, ethanolic extract, total phenolics, antioxidant capacity
58
CYP2E1 GENOTYPE AND TRANSAMINASE LEVEL OF TUBERCULOSIS PATIENTS
RECEIVING FIXED DOSE COMBINATION OF ANTITUBERCULOSIS
I Gusti Ayu Artini1, I Gusti Ngurah Bagus Artana
2, I Gusti Made Aman
1, Agung Nova
Mahendra1
1Pharmacology Department, Medical Faculty, Udayana University, Indonesia
2Internal Medicine Department, Medical Faculty, Udayana University, Indonesia
ABSTRACT
Introductions: Antituberculosis drug-induced liver injury (ATLI) had become a common
serious side effect regarding antituberculosis use. Isoniazid (INH) was believed as a crucial
factor related to ATLI incidence and a genetic factor related to INH metabolism (eg. CYP2E1)
was assumed as an important contributor of ATLI. This study aimed to investigate the genotype
pattern of CYP2E1 and serum transaminase level on tuberculosis patients receiving fixed-dose
combination of antituberculosis. Methods: As many as 35 tuberculosis patients attending
Pulmonary Outpatient Clinic of Sanglah Hospital were included in this cross-sectional study.
Identification of CYP2E1 genotype was performed with PCR-RFLP assay using RsaI and DraI
restriction enzyme. Results: This study revealed the proportion of c1/c1; c1/c2; and c2/c2
genotype of CYP2E1 on 5’-flanking region were 62.9%; 34.3%; and 2.8%, respectively; whereas
the proportion of DD, CD and CC genotype of CYP2E1 on intron 6 were 60%; 28.6%; and
11.4%, respectively. The proportion of hepatotoxicity was 14.3%, while the mean value of AST
and ALT level were 23.5±13.6 IU/L and 23.3±21.1 IU/L. Conclusion: The dominant proportion
of CYP2E1 genotype on 5’-flanking region and intron 6 were c1/c1 and DD.
Keyword: antituberculosis drug-induced liver injury, isoniazid, CYP2E1 genotype
59
PHARMACO-ETHOLOGICAL AND HIPPOCAMPAL CA1 REGION
NEUROHISTOLOGICAL STUDY OF SERTRALINE EFFECTS ON SINGLE-
ROLONGED STRESS-INDUCED RODENT MODEL OF PTSD
Agung Nova Mahendra,1,2*
I Nyoman Tri Pramartha,3 Ni Luh Sari Darmayanti,
3
Ni Wayan Sucindra Dewi1
Department of Pharmacology and Therapy,1 and Division of Drug Development and Laboratory
Animal, Integrated Biomedical Laboratory Unit,2 and Undergraduate Student of Physician
Education Study Program (PSPD),3 Faculty of Medicine, Udayana University, Bali, Indonesia
*Correspondence: Agung Nova Mahendra, MD, M.Sc.
Faculty of Medicine, Udayana University, Bali, Indonesia
E-mail: [email protected]
ABSTRACT Background: Sertraline is one of several drugs approved for posttraumatic stress disorder
(PTSD), an anxiety disorder which is often accompanied by hippocampal defect. Hippocampal
CA1 region is known to be associated with anxiety. Single-prolonged stress (SPS)-treated rats, a
rodent model of PTSD, has been found to induce apoptosis of hippocampal pyramidal neurons.
To our knowledge, the effect of sertraline on anxiety-like behaviors and hippocampal CA1
neurons had not been studied in SPS-induced rats as a model of PTSD. The aim of this study was
to investigate the effect of sertraline on classical and ethological variables of anxiety-like
behavior, and histomorphological appearance of hippocampal CA1 region in SPS-induced rats.
Methods: Wistar male rats (8-10 weeks; 150-180 g [n = 35]), were randomized into 5 groups.
Group 1: Normal control. Group 2: Rats induced with SPS for 7 days + 7 days of consolidation.
Group 3: Rats induced with SPS for 7 days + 7 days of consolidation + sertraline 4.5 mg/kg/day
(orally). Group 4: Rats induced with SPS for 7 days + 7 days of consolidation + sertraline 9
mg/kg/day (orally). Group 5: Rats induced with SPS for 7 days + 7 days of consolidation +
sertraline 18 mg/kg/day (orally). Sertraline was administered for 14 days. Behavioral measures
were studied with elevated plus maze test. Hippocampus-containing coronal slices of the brains
were HE-stained and analyzed using Optilab. Results: The numbers of closed arm entries and
stretched-attend posture (SAP) were not different between groups. Sertraline decreased time
spent in closed arms, increased open arm entries, time spent in open arms, and unprotected head
dips (UHD). Lowest dose of sertraline exerted highest level of anxiety as evidenced by the
number of protected head dips (PHD), but higher doses ameliorated this effect. Spatial
arrangement of CA1 pyramidal neurons was severely impaired in all SPS-exposed groups.
Pyknotic, dark-stained, and shrinked neurons were commonly found in sertraline-treated rats,
especially at the dose of 18 mg/kg/day. Conclusions: We found that chronic administration of
sertraline exerts anxiolytic effect on single-prolonged stress-induced rats. Increasing dose of
sertraline is associated with increasing “chaotic” appearance of hippocampal CA1 region
histomorphology.
Keywords: Sertraline, pharmaco-ethological analysis, hippocampal CA1 region, single-
prolonged stress, rats
60
DETERMINANTS OF POST TRAUMATIC STRESS DISORDER (PTSD)
AMONG TRAFFIC ACCIDENT VICTIMS IN BALI – INDONESIA
Putu Aryani1, Cokorda Bagus Jaya Lesmana2, Pande Putu Januraga3,
Komang Ayu Kartika Sari1, Nyoman Sutarsa1
1Department of Community and Preventive Medicine, Faculty of Medicine, Udayana University, 2 Department of Psychiatric,
Faculty of Medicine, Udayana University, 3School of Public Health, Faculty of Medicine, Udayana University
ABSTRACT
Background: The incidence of traffic accident in Bali has increased over the last decade. The
mortality rate remains high – contributing to more than 500 deaths annually between 2011 and
2013. Studies have revealed that traffic accident victims are more vulnerable to experience post-
traumatic stress disorder (PTSD). This however is rarely been explored in the context of eastern
culture society especially among the Balinese. This study was aimed at exploring the incidence
and determinants of PTSD among traffic accident victims in Bali Province. Methods: A cross
sectional analytic study was conducted to explore incidence and severity of PTSD among traffic
accident victims in Bali Province between 2015 and 2016. A total of 153 respondents were
selected using an accidental technique. Data were collected using a structured interview guided
by structured questionnaires. The international questionnaire (PCL-C V) to screen the symptom
of PTSD for traffic accident victims was adopted. Data were analyzed by applying statistical
analyses. Results: The majority of respondents were 18-27 years (65.4%), female (62.1%),
Balinese (87.6%), Hindu (85.6%), being single (64.1%), high school graduated (50.3%),
employed (62.2%), and living with the extended family (42.5%). Prevalence of PTSD among
traffic accident victims was only 1.9%. It might be associated to the late screening time where
the majority of respondents were interviewed a month or more after the accident (71.5%). As
many as 46.2% respondents reported a mild symptom of PTSD. Higher proportion was found
among younger age, female, low educational level, severe injuries and longer hospitalization.
Conclusion: The prevalence of PTSD among traffic accident victims was very low which could
be underestimated due to the late screening process. An early screening and a prompt treatment
are required to facilitate coping strategies among traumatic victims.
61
SUPPLEMENTATION 2000MG AND 1000MG OF LINOLEIC ACID AND
ALFA LINOLENIC ACID DELAYED PREDIABETIC STATE IN
BALINESE YOUNG OBESE WOMEN: A RANDOMISED CLINICAL
TRIAL I W Weta
1, Wayan P Sutirtayasa
2, Anak Agung N Subawa
3, Safarina G Malik
4.
1 Department of Community and Preventive Medicine/Department of Clinical Nutrition, Facullty of Medecine,
Udayana University/Sanglah Hospital, Denpasar Bali Indonesia. 2
Study Program Doctor, Post Graduate Program Faculty of Medicine Udayana University, Denpasar Bali
Indonesia. 3 Department of Clinical Pathology Faculty of Medicine Udayana University Denpasar Bali Indonesia.
4 Eijkman Institute for Molecular Biology, Ministry of Research Technology and Higher Education, Republic of
Indonesia, Jakarta, Indonesia
Corresponding Author: Dr I Wayan Weta, Department of Preventive and Community Medicine/Clinical Nutrition,
Faculty of Medicine, Udayana University/Sanglah Hospital, Jl. PB Sudirman, Denpasar, Bali 80232, Indonesia.
Tel: +62 81337005360; Fax: +620361246656
Email: [email protected]
ABSTRACT
Background. Obesity fuelled epidemic of type 2 diabetes mellitus, globally. Controlling
prediabetic state in obese person is important to prevent the type 2 diabetes. PUFAs (n-6 and n-
3) have a role in controlling insulin resistance. The aim is to elucidate the effect of the linoleic
acid (LA) and alfa linolenic acid (ALA) supplementation to fasting plasma glucose in young
women obese patients. Material and Method. In totally, 45 young obese women in Denpasar
Bali, randomized into two group; 22 participants were supplemented with 2000mg and 1000mg
of LA and ALA (Intervention), and 23 participants were given placebo (Control), for 12 weeks.
Both groups were recommended to restrict their daily energy intake <1500 kcal, during the
study. The data namely; dietary, anthropomeric, blood triglyceride, plasma fasting glucose were
assessed in base-line and end-line of study. Results. Both groups resticted their energy intake
less than 1500 kcal in the endline assessment. All of body fat parameters (BMI, triglycreide,
waist circumference, conisity index, waist to height ratio, and lipid accumulation product;
p=0.003, p=0.049, p<0.001, p=0.002, p=0.001, and p= 0.001, respectively) decreased in the
Intervention group. However, only the conisity index decreased (p=0.025) in the Control group.
In the Intervention group no significant change of fasting plasma glukose, contrary it increased
(p=0.007) in the Control group. The impaired plasma glucose increased in the Control group
(OR=8, p=0.039), but not in the Intervention group (p=0.508). Conclusion. Restriction energy
intake with supplementation 2000mg and 1000mg of LA and ALA control prediabetes of young
obese women.
Key words: alfa linolenic acid, fasting plasma glukose, prediabetes, young obese women.
62
THE ANTICANCER ACTIVITY OF (E)-1-(4’-AMINOPHENYL)-3-PHENYLPROP-2-
EN-1-ON AGAINST DMBA-INDUCED MAMMARY CANCER IN SPRAGUE DAWLEY
RAT THROUGH THE REGULATION OF MICRORNA-21 EXPRESSION
Ida Ayu Ika Wahyuniari1, I G K Nyoman Arijana
1, Ni Putu Sriwidyani
2, Ida Ayu Dewi
Wiryanthini3, Mustofa
4, Muhammad Ghufron
5, Sitarina Widyarini
6, Sofia Mubarika
5
1Department of Histology, Faculty of Medicine, Udayana University, Bali,
2Department of Anatomical Pathology, Faculty of Medicine, Udayana University, Bali,
3Department of Biochemistry, Faculty of Medicine, Udayana University, Bali,
4Department of Pharmacology and Therapy, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta,
5Department of Histology and Cell Biology, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta,
6Department of Anatomical Pathology, Faculty of Veterinary Science, Universitas Gadjah Mada,
Yogyakarta, Indonesia.
Correspondence to Ida Ayu Ika Wahyuniari, Department of Histology, Faculty of Medicine, Udayana
University, Jalan PB. Sudirman, Denpasar, Bali, 80237, Indonesia
email : [email protected]
ABSTRACT
Background: The new anticancer is urgently needed due to the high resistance and recurrence of
breast cancer. Previous study reported that a new chalcone derivative, (e)-1-(4’-aminophenyl)-3-
phenylprop-2-en-1-on, has a potential cytotoxicity against T47D breast cancer cell line. In this
study we investigated the anticancer activity of (e)-1-(4’-aminophenyl)-3-phenylprop-2-en-1-on
against DMBA-induced mammary cancer in Sprague Dawley rat and its effect on microRNA-21
expression. Methods: Twenty four female rat were divided into 6 groups. The first group, G1 as
negative control. The groups 2 to 6 (G2, G3, T1, T2, and T3) were induced by DMBA 20
mg/kgBW for 5 weeks. After breast nodule observed, G2 as vehicle-treated group and G3 as
positive control received tamoxifen, respectively. Whereas, T1, T2, T3 received (e)-1-(4’-
aminophenyl)-3-phenylprop-2-en-1-on with different doses, ie. 5, 15, and 45 mg/kgBW/day for
21 days, respectively. Tumor size was measured every weeks for 21 days and on day 22, plasma
and breast tissues were collected to examine the miR-21 expression by qRT-PCR and
histopathological feature, respectively. Result: The result showed that significantly decreased
tumor growth (p<0.05) and better histopathological malignant grading in T1, T2, T3 were
observed. Moreover, significantly reduced miR-21 relative expression (p<0.05) in T1 and T2
were also observed. Conclusion: (e)-1-(4’-aminophenyl)-3-phenylprop-2-en-1-on has potential
anticancer activity on DMBA-induced mammary cancer in Sprague Dawley rat through its
activity on miR-21 expression. Hence, (e)-1-(4’-aminophenyl)-3-phenylprop-2-en-1-on might be
a new anticancer candidate in the future.
Keywords: (e)-1-(4’-aminophenyl)-3-phenylprop-2-en-1-on, tumor growth, histopathology,
microRNA-21 expression, breast cancer
63
PREPARATION AND IN VITRO CHARACTERIZATION OF SELF-NANO
EMULSIFYING SYSTEM OF C- PHENYLCALIX-[4]-RESORCINARYL
OCTACINNAMATE AND C-METHYLCALIX-[4]-RESORCINARYL
OCTABENZOATE AS ULTRAVIOLET ABSORBERS
Indrayani Agung Wiwiek1, Suwaldi Martodihardjo
2, Soenardi
3, Jumina
4, I Gusti M
Ngurah Budiana5, Mustofa
6
1Department of Pharmacology, Faculty of Medicine, Udayana University, PB.Sudirman, Bali 80237, Indonesia
2Department of Pharmaceutics, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281
3Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara,
Yogyakarta 55281 4Department of Skin and Venerelogy, Faculty of Medicine, Universitas Gadjah Mada, Bulaksumur Yogyakarta
55281, Indonesia 5Departement of Chemistry, Faculty of Education and Teacher Training, Nusa Cendana University, Kupang 85001,
Indonesia 6Department of Pharmacology and Therapy, Faculty of Medicine, Universitas Gadjah Mada, Bulaksumur
Yogyakarta 55281, Indonesia
Address of Correspondence: Agung Wiwiek Indrayani Department of Pharmacology, Faculty of Medicine,
Universitas Udayana, Jl. PB.Sudirman Bali 80237, Indonesia Email: [email protected]
ABSTRACT
Background: Sunscreens are cosmetic products which are used to protect the skin from
ultraviolet (UV) rays exposure. Ultraviolet rays, especially ultraviolet B (UVB) rays can cause
skin cancer. C-phenylcalix-[4]-resorcinaryl octacinnamate (compound 1) and C-methylcalix-[4]-
resorcinaryl octabenzoate (compound 2) are newly synthesized compounds which have high
absorbance of UVB rays. Despite their potential activity, these compounds have low solubility in
water. Therefore, in order to increase the solubility, they were formulated into a nanoemulsion.
Objective: The aim of the present study was to prepare and characterize a self-nano emulsifying
system of two compounds as UV absorber. Methods: Optimum formula of nanoemulsion was
analyzed using D-optimal mixture design with Design Expert® ver. 7.1.5 by varying
concentrations of candlenut oil, tween 80, and polyethylen glycol 400 (v/v). Sun Protection
Factor (SPF), transmittance and time of emulsification were evaluated as optimization
parameters. The optimal formula was further evaluated for its distribution, droplet size, and zeta
potential. Results: A combination of two compounds was used in formulations and has SPF
value of 52.189. The composition of optimal formula was obtained at 2.5 mL of candlenut oil,
5.066 mL of tween 80, and 2.434 mL of polyethylene glycol 400. The results showed that the
optimal formula has SPF value of 81.577, % of transmittance 90.84, emulsification time of 17.42
seconds, desirability of 0.726, average droplet size of 129.9 nm with polydispersity index 0.393
and zeta potential of -15.2 mV. Conclusion: Nanoemulsion formulation using candlenut oil
could increase SPF value of sunscreen compounds and potentially be used as alternative UV
absorbers dosage forms in the future.
Keywords: self-nanoemulsifying system, formulation, C-phenylcalix-[4]-resorcinaryl
octacinnamate, C-methylcalix -[4]-resorcinaryl octabenzoate, UV absorbers, candlenut oil.
64
DETECTION OF CITRUS VEIN PHLOEM DEGENERATION (CVPD)
DISEASE AND THE QUALITY OF HEALTHY FRUITS IN NUTRIENT
DEFICIENCY OF CITRUS
I Gede Putu Wirawan*, Sheila Simanjuntak, Made Sritamin, and
Nyoman Wijaya
Graduate School of Agricultural Biotechnology, Faculty of Agriculture, Udayana University
Jalan. PB. Sudirman. Denpasar 80232 Bali. Email: [email protected]
ABSTRACT
Background: Citrus plantation has a main disease called citrus greening disease or
Huanglongbing or Citrus Vein Phloem Degeneration (CVPD), and in Indonesia is wellknown as
CVPD disease. The disease caused by Gram negative bacteria called Liberobacter asiaticus. The
disease symptom is very similar to plant deficiency of nuitrients and can be confirmed by using
polymerase chain reaction (PCR). Objective: This study try to find the nutrients deficiency,
quality of the fruits, and to detect the present of the pathogenic bacteria, L. asiaticus. Methods:
In the study of nutrients deviciency was done by using AAS technique, while the study of the
healthy and consumable fruits was done by using AAS and HPLC techniques. Detection of L.
asiaticus we used the PCR method. Results: The results of this study showed that all citrus
plants showing the nutrients deficiency were infected by the pathogenic bacteria. Nutrient
deficiency of citrus plants were found to be deficiency in Fe, Mn, Mg, or Zn and the quality of
the fruits therefore, were physically small and lack of vitamin C and antioxidances. Conclusion:
The results indicated that there is a correlation between the citrus plant’s infected by L. asiaticus
caused nutrients deficiency and lead to the quality of the fruits that we consume.
Keywords: Citrus plants, nutrient deficiency, Liberibacter asiaticus, PCR, AAS
65
MUTATION IDENTIFICATION OF RPOB GENE FROM MDR-TB ISOLATES AT
SANGLAH HOSPITAL DENPASAR, BALI
Dwija, Ida Bagus Nyoman Putra, Ni Made Adi Tarini, Ida Ayu Kade Ratna
Sukmaningsih, Wahyu Hidayati
Microbiology Department, Faculty of Medicine Udayana University, Bali.
Corresponding author: [email protected]
ABSTRACT
Tuberculosis (Tb) that caused by Mycobacterium tuberculosis (Mtb) bacteria is one of the
leading infections that caused high mortality rate annually. Anti-mycobacterium drugs is almost
successful to control the burden of diseases, but emergence of drug resistance bacteria such as
Multi Drug Resistance (MDR-) or Extended Drug Resistance ( XDR-) tuberculosis is an another
problem faced today. Genetic change or mutation is one of the mechanisms that bacteria can be
resistance to one or more drugs. RpoB gene is one target of most effective drugs: rifampicin,
changes within this gene will make drugs is not effective. The aim of this research is to explore
the RpoB gene from the multidrug resistance Mtb isolate which is isolated from TB suspected
patient. Mtb isolated then observe both phenotype and genetic. As much as 30 sputum specimen
was collected, all of them is examine by microscopic and further culture and DNA Extraction for
PCR using specific primer. Among 30 specimens has been collected, all of them is AFB smears
positive. Drugs susceptibility testing result is vary, but all of them is Rifampicin resistance,
followed by resistance to one or more other drugs. Sequence analysis show that mutation has
found is nucleotide change and insertion.
Keywords : MDR, RpoB, Mutation, Sanglah
66
HYPOGLYCAEMIC EFFECTS OF ZYZYGUM POLYANTHUM LEAVES EXTRACT
ON ALLOXAN-INDUCED THROUGH DECREASE OF BLOOD GLUCOSE AND 8-
HYDROXY-2 DEOXYGUANOSINE (8-OHDG) IN HYPERGLYCEMIC WISTAR RATS
Sri Wahjuni 1; I Wyn Wita
2
1 Departement of Chemistry,Faculty of Mathematic and Natural Science Udayana University Campus Bukit
Jimbaran street 80361 Bali Indonesia 2 Clinical Internal Departement, Faculty of Medicine, Udayana University, PB. Sudirman Street Denpasar Bali
Indonesia
ABSTRACT
Hyperglyemia is a condition with an increase of blood glucose fasting levels (above 109 mg/dL)
and 2-hour blood glucose post prandial (above 158 mg/dL). Hyperglycemia increases reactive
oxygen species (ROS) through the process of enzymatic reactions. This reaction includes
oxidation and phosphorylation and ADPH-Oxidase reaction and through non-enzymatic process
by forming of Gluco oxidants and Glycation . Hyperglycemia is caused by abnormalities in
insulin secretion or action of insulin disorders. The state of hyperglycemia in diabetes lead to
increase formation of free radicals, antioxidants and decrease a number of events that eventually
occurs is called oxidative stress. Hyperglycemia can induce an increase in free radicals through
glucose auto-oxidation, the formation of Advanced Glycation End Products (AGES), and incre
ase of polyol pathway activity (sorbitol). The process outooxidation non hyperglycemia and
glycation reactions result in the release of electrons. The release of the electrons will trigger the
formati on of free radicals (RB) particulary superoxide radicals (O2), and hydrogen peroxide
(H2O2) and via Haber-Weis and Feton will form hydroxyl radicals (OH.). These materials are
known as oxygen free radicals ( RBO), which scan damage cell membranes (8-OHdG) and
glucose blood are known to diabetic Wistar Rats. Zyzygum polyanthum leaves extract rich in
phytol and phytol acetat is the same chlorofil. Extract zyzygum polyanthum has an ability to
prevent hiperglicemic disease. This is caused by the present of phytol and phytol acetat in the
zyzygum polyanthum leaves extract which has been phytol-chemistry tested and analyzed by
GC-MS such as phytol and phytol acetat substance.This research aimed to study the effect of
zyzygum polyanthum leaves extract in inhibiting stress oxidative in hyperglycemic. A
randomized pre-test and posttest control group design was performed on 40Wistar rats by
analyzing blood glucose,8-Hydroxy-2 Deoxiguanosin (8OHdG). Extraction of 3 kg zyzygum
polyanthum leaves ) with etanol gave 0,730 kg crude extract. Hyperglycemic on Wistar rats was
made by inducing hyperglycemic with alloxan 2 week. The experimental was performed on five
groups of animals that were Hyperglycemic: P0 for normal rats feed standar (control positip),P1
for normal rats alloxan induced with not intake zyzygum polyanthum (control negatip), P2 for
hyperglycemia rats alloxan induced with intake zyzygum polyanthum leaves extract 0.5 mg kg-1
body weight, P3 for hyperglycemia rats allozan induced with intake zyzygum polyanthum
leaves extract 2.0 mLkg-1 body weight; P4 for hyperglycemia rats alloxan induced with intake
zyzygum polyanthum leves extract 5.0 mLkg-1 body weight. P5 for hyperglycemia rats alloxan
induced with treatment glibenclamide 0,18 mg/day/200g body weight. The result showed that
intake of zyzygum polyanthum leaves extract in 5,0 mLkg-1
body given have decreased the
activity of blood glucose 65.91 %, decreased 8-OHdG 50.20%.
Keywords: Zyzygum polyanthum leaves extract extract; blood glucose; 8-Hydroxy-2
Deoxyguanosine ( 8-OHdG)
67
COMMITTEE
Advisors
Prof. Dr. dr. Putu Astawa, SpOT (K), M.Kes
Prof. Dr. dr. I Gede Putu Adiatmika, M.Kes
Executive Committee
Chairman : Dr. rer. nat. dr. Ni Nyoman Ayu Dewi, M.Si.
Representative chairman : dr. I Wayan Surudarma, M.Si
Secretary : dr. Agung Nova Mahendra, M.Sc
Treasurer : Nyoman Sri Handayani, S.Si
Event Division
Seminar :
Dr. dr. Desak Made Wihandani, M.Kes
dr. I Kadek Swastika, M.Kes
Ni Komang Tri Widianingsih, A.Md, A.K
I.A. Kade Ratna Sukmadewi, A.Md, A.K
Molecular Biology Workshop :
I.B.N. Putra Dwija, S.Si., M.Biotech.
Ni Ketut Nanik Astuti, S.Si
Ni Putu Senshi Septiasari, S.Si., M.Si
I Gd Wayan Topan Yogi Dyana, ST.
ELISA Workshop :
Dr. dr. I Nyoman Wande, SpPK
Amy Yelly Kusmawati, SKM, MP.
I Ketut Gede Adi Santika, A.Md, A.K
Poster :
dr. I G Kamasan Nyoman Arijana, M.Si.Med
I Made Angga Baskara, A.Md, A.K
Secretariat and Public Relation :
dr. Putu Gede Supadmanaba, S.Ked.
dr. IA. Dewi Wiryanthini, M.Biomed
I Made Indrawarma, S.Kom
Consumption :
Ni Wayan Sudarni
Ni Wayan Noni
Ni Wayan Niti
68
Equipment :
dr. Wayan Gede Sutadarma, SpGK
I Dewa Ketut Alit Sumarsana, Amd.
I Made Arjana, Amd.
Gede Wiranatha, S.Si.
I Dewa Gede Wira Satya Utama, S.Kom
I Made Suardana
Documentation :
I Nyoman Agus Tinajaya
A.A. Ngurah Mayun S.T
Transportation :
Drs. I Ketut Kartika
I Wayan Adnyana Wijaya, S.P