production of insulin from microbial culture
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TRANSCRIPT
PRODUCTION OF INSULIN FROM MICROBIAL CULTURE
RUI BAIMUHAMMAD AKLIL BIN AYOB
YANGJIE CUI
CHEN 60351 : CORE BIOTECHNOLOGY 1
CONTENT1. Abstract2. Introduction3. Main process of insulin production4. Significance of human insulin5. Conclusion
Abstract Hormone secreted by pancreas Control blood sugar level Lack of insulin caused diabetes
mellitus Industrial production by
manipulating genetic engineering and microbial culture technology
Introduction Protein generated
by beta cell of pancreas (islet of Langerhans)
Exist in animal: porcine and bovine
Build up of 51 amino acids
30 amino acids form chain B
21 amino acids form chain A
2 chain linked by disulfide bonds
(Introduction)…cont
www.bio.davidson.edu
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(Introduction)…cont Diabetes mellitus
(DM) lack of insulin to control blood sugar level
Need to insulin therapy to keep healthy
www.som.uq.edu.au
Main process of insulin production Microorganisms
Fungi Yeast
www.google.com.hk
Microorganisms
Kinds of bacteria E. coli
www.google.com.hk
Genetic Engineering
http://blogs.swa-jkt.com/swa/10822/
Microbial Fermentation
Significance of human insulin 1978: insulin from animal; pig and cow Switch to microbial culture. Why?
Quantity & quality is better No allergy and side effect
E. coli culture is convenient Cheaper for commercial
Predictable Problems easily solved
Conclusion Insulin market grown at
7% annual rate Company: Novo
Nordisk, Eli Lilly & Sanofi Aventis
Cost : £75 – 200 per vial Different delivery ways
www.novonordisk.com
http://www.bd.com
Thank you!