production of biofeeds, biofuels and biochemicals from co2 ... yukawa.pdf · 2017 bio world...

5.3 Microbial and Synthetic Approaches to CO2 Utilization,

2017 BIO World Congress

Dr. Hideaki YukawaCEO, Utilization of Carbon Dioxide Institute Co., Ltd.

Production of Biofeeds, Biofuels and Biochemicalsfrom CO2 using Hydrogen Bacteria

July 24, 2017

Copyright© 2017 by UCDI All Rights reserved. 1

UCDI’s Business Plan

Convert CO2 into high-value products

Contribution to food security and global warming problems


UCDI’s Platform: Hydrogen Bacteria

Hydrogenophilus thermolutelus


What are Hydrogen Bacteria?

Conclusion: 20L-culture could support one Astronaut


Biotech. and Bioengi. vol-8,217(1966)

NASA: Exploring the possibility of using ”Hydrogen Bacteria” as

a protein production for space flight


History of UCDI’s Hydrogen Bacteria

Isolation from Nature

1970s: Dr. Kodama’s team, Applied Microbiology Lab., University of Tokyo, had tried to isolate many kinds of hydrogen bacteria from nature.

They set up a landmark of hydrogen bacteria’s feature for future industrialization.

* Rapid growth in a complete inorganic medium Target of doubling time : less than 1 hour

* Thermophilic hydrogen bacteriaTarget of optimum temperature: over 50˚C

Scientific AdvisorDr. Toru Kodama


History of UCDI’s Hydrogen Bacteria

Screening Result1976: Dr. Kodama’s team got big success

The strain’s feature• Doubling time: 1hour• Optimum temperature: 52˚C• Optimum pH: 7.0•Gram- negative• Strictly aerobic

Taxonomic position: Pseudomonas hydrogenothermophilaAgric. Biol. Chem., 41,685 (1977)

By the detailed taxonomic study: Hydrogenophilus thermolutelusInt. J. Syst Bacteriol.,49,783 (1999)

Tokyo

IzuPeninsula

The targeted strain was isolated from a hot spring in Izu Peninsula


[archive] History of UCDI’s Hydrogen Bacteria

“Hydrogen Bacteria” is rich nutrition• Protein content: 80%• Continuous production process is

practicable• Feed test using poultry was done

successfully

Social cartoon“Dinner in future”

“Hydrogen Bacteria” on the dinner table

Japanese News Paper “Asahi “ took up the research project in both article and social cartoon(1978)


World Population Forecast

This sharp increase in global population is the root cause of various issues facing our planet, including environmental pollution and food security.

Now: More than 7 B people inhabit our planet

Future: Over 9B or close to 10 B by the year 2050

Source: Ministry of Agriculture, Forestry and Fisheries


Biofeeds: Single Cell Protein

UCDI strain Hydrogenophilusthermolutelus

• High growthrate: dubling time 1hour

• High Amino Value• Non-GMO• Natural protein source

High protein-score/ High cell density

Before inoculation 10hrs incubation

Hydrogenophilusthermolutelus


Biofeeds: Production Process


Biofeeds: Aquaculture Fields

Fishers and aquaculture:

Important sources of food and nutrition

World per capita fish supply

20kg in 2014 Thanks to Aquaculture

Aquaculture provides half of all fish

Source: FAO Fish State


How to keep sustainable aquaculture?

Fish feed: the biggest cost in the

production of aquaculture

Fishmeal is main ingredient

50~70%W/W in fish feed

Fishmeal Production and Price

Plant engineering : UCDI collaborating with major engineering companies


UCDI’s Biofeeds : Business Plan

2014 2025 Growth rate

Chicken 88.4 113.0 28%

Pork 111.2 133.0 20%

(Million ton)Supply and Demand

Market : aquaculture, chicken, pork

Source: Policy Research Institute, Ministry of Agriculture, Forestry and Fisheries (PRIMAFF)


rDNA Research

Established main generic tools

• Genetic manipulation both in autotrophic and heterotrophic culture• Gene transfer

Plasmid vectorsIntroduction into chromosome by homologous recombination

• Transformation by electroporation• Selection

Positive selection by antibiotics resistance kanamycin, hygromycin, etc.

Negative selection with sacB gene• Reporter gene

Beta-galactosidase from Thermus therm ophilus

(e.g. for screening of promoters)

Counter selection


rDNA Research

Set up ” Industrial host cell ” for cell-factory

Business Plan

Butanol : Material for Jet Fuel

Amino Acids: Feed Additives (Trp, Met. etc.)

Organic Acids/Aromatic compounds : Materials for Polymer


Biocatalysts: genetically modified strains

We are looking for business partners !!

Amino Acid

Established : August 12, 2015

Paid-in Capital : US$1M

Head Office : Minato-ku, Tokyo

Representative : Dr. Hideaki Yukawa, CEO

Research Facility : Campus of University of Tokyo

Mission : Contribution to Food Security and Global

Warming Problems


About Us


Scientific Advisor

Dr. Toru Kodama

Special Advisor

Dr. Kazuo Nagai

Director

Ms. Tomoko YukawaExecutive Officer

Mr. Hiroshi Maeda

Manager

Ms. Setsuko Beppu

Manager

Ms. Sumie HonmaManager

Ms. Hikari Miyazaki

Manager

Ms. Ryoko Senoo

CEO

Dr. Hideaki Yukawa

Head Office


Research Team

rDNA works

Fermentation works

Senior Researcher

Dr. Naoto OhtaniResearcher

Dr. Kenro ShinagawaResearcher

Dr. Shota Oku

Alignment :Applied Microbiology Lab. University of Tokyo

Professor, PhD

Masaharu Ishii


Thank youPlease contact to

[email protected]

production of biofeeds, biofuels and biochemicals from co2 ... yukawa.pdf · 2017 bio world...

Documents