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Prezentci cme 10/25/13 Electrolytically modified functional water (Kaqun) as a clinically proven adjuvant treatment in cancer patients www.kaqun.eu JNOS HUNYADI MD DScSANDOR KULIN MD

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Prezentci cme 10/25/13 What is KAQUN? KAQUN is a special type of high oxygen- content water, which can be used as water for therapy by drinking and/or bathing. Technology used: electrolysis nothing is added to water

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Prezentci cme 10/25/13 Kaqun Hungria Ltd. has been using electrolysis- produced, high oxygen-content water since 2001 for the purpose of improving well-being, in form of drinking and bathing therapy. Last 10 years 10 completed licensed scientific study

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Prezentci cme 10/25/13 Percentage ratio of NK cells increased The expression of CD25 surface marker increased Increased reactive oxygen intermediates production improves activity of neutrophil granulocytes IMMUNOLOGICAL EXAMINATIONS (DEPARTMENT OF CYTOGENETICS AND IMMUNOLOGY OF THE NATIONAL INSTITUTE OF CHEMICAL SAFETY)

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Prezentci cme 10/25/13 The effects of KAQUN-water on patients undergoing oncological treatment. A randomized study National Instiute of Oncology Budapest, Hungary 100 patients with Melanoma Malignum stage IV

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Prezentci cme 10/25/13 To study the effects of Kaqun-water on patients undergoing oncology treatment Primary endpoints: Radiation-induced dermatitis Drug-induced dermatitis Fitness and state of mind Studying changes in exulceration Secondary endpoints: Changes in cutaneous manifestations of melanoma Tumor response in complex oncotherapy of stage IV melanoma

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Prezentci cme 10/25/13 EFFECT ON DERMATITIS CAUSED BY RT 3. days of 1st KAQUN- therapy 14. days of 1st KAQUN- therapy Last day of 3rd KAQUN-therapy

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Prezentci cme 10/25/13 EFFECT ON DERMATITIS CAUSED BY RT Before KAQUN-therapyAfter 10 daysAfter 7 daysAfter 3 days

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Prezentci cme 10/25/13 Investigation of electrolysed functional (Kaqun) water on growth of human ovarian carcinoma (A2780), and human cervix carcinoma (KB-3-1) xenografts in SCID mice. JNOS HUNYADI MD Department of Dermatology, University of Debrecen, Hungary National Institute of Oncology, Budapest, Hungary

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Prezentci cme 10/25/13 Human cervix carcinoma cells KB-3-1 Human ovarium carcinoma cells A2780

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Prezentci cme 10/25/13 Cervix, control, 16 days

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Prezentci cme 10/25/13 Cervix, Kaqun 16 days

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Prezentci cme 10/25/13 Ovary, control 16 days

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Prezentci cme 10/25/13 Ovary, Kaqun 16 days

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Prezentci cme 10/25/13 Overall observations There is a remarkable difference between the two investigated tumors. Growing of A2780 cell tumors were in a 7 days delay compared to that of KB-3-1 cells Four out of the 9 control mice died before finishing the experiment, while only 2 out of the 14 Kaqun consuming groups did so. Seventeen days after xenotransplantation the volume of tumor was significantly smaller in Kaqun and pre-Kaqun group in the case of both types of tumors if the injected cells were 4 million.

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Prezentci cme 10/25/13 Growth of tumors after 4 X 106 A2780 tumor cell transplantation was not detected in 3 of 14 mice in the Kaqun treated groups, as opposed to control mice (n=9) where growing tumors were detected in each of them..

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Prezentci cme 10/25/13 The most significant ovarian carcinoma inhibiting effect was seen in the group of 2 X 106 A2780 cell transplanted mice, when starting Kaqun water consumption 14 days before xenotransplantation.

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Prezentci cme 10/25/13 In 2 X 106 KB-3-1 tumor cell transplanted, Kaqun water consuming animals in 5 out of 14 mice, a tendency of tumor-regression was observed.

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Prezentci cme 10/25/13 Karuppaiyah Selvendiran, M. Lakshmi Kuppusamy, Shabnam Ahmed, Anna Bratasz, Guruguhan Meenakshisundaram, Brian K. Rivera, Mahmood Khan and Periannan Kuppusamy: Oxygenation inhibits ovarian tumor growth by downregulating STAT3 and cyclin-D1 expressions. Cancer Biology & Therapy 10:4, 386-390; August 15, 2010; 2010 Landes Bioscience

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Prezentci cme 10/25/13 Physiological background of KAQUN actions Sndor Kulin MD Orlando 2013

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Prezentci cme 10/25/13 The key to understanding: Mitochondria

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Prezentci cme 10/25/13 "Cancer, above all other diseases, has countless secondary causes. But, even for cancer, there is only one prime cause. Summarized in a few words, the prime cause of cancer is the replacement of the respiration of oxygen in normal body cells by a fermentation of sugar " Dr. Otto H. Warburg From his Nobel Lecture

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Prezentci cme 10/25/13 Electron transport chain ATP - Energy - Membrane Potential Redox Signaling Molecules CO2 Production

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Prezentci cme 10/25/13 Healthy cells: -70mV Chronic inflammation: < -50 mV Cancer: < -20 mV Death: 0 mV Membrane potential

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Prezentci cme 10/25/13 Low membrane potential (below -20 mV) is a prerequisite and a trigger of cell division. Even healthy cells decrease membrane potential below this threshold to perform cell division. There is no cell division above this value! Because of lack of energy, cancer cells can not increase membrane potential - so they remain in the division phase all the time The lower the membrane potential the higher the division rate! There is a strong link between membrane potential values and clinical malignancy.

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Prezentci cme 10/25/13 Electron transport chain ATP - Energy - Membrane Potential Redox Signaling Molecules CO2 Production

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Prezentci cme 10/25/13 Redox signaling molecules have regulating effects on o gene expression, o apoptosis, o cell growth, o cell adhesion, o chemotaxis, o angiogenesis, o immune reactions, o inflammation. As the primary source of redox signaling molecules are the mitochondria - in case of mitochondrial dysfunction, all these functions are affected

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Prezentci cme 10/25/13 Electron transport chain ATP - Energy - Membrane Potential Redox Signaling Molecules CO2 Production

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Prezentci cme 10/25/13 In lack of mitochondrial activity there is no CO2 production in cancer tissues. In lack of pCO2, Oxygen remains attached to Hemoglobin - inspite of the rich blood supply of the tumor, cancer tissue remains hypoxic. In lack of Oxygen, mitochondria can not be restarted. Any method that brings Oxygen into cancer cells and restarts mitochondria is curative to cancer.

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Prezentci cme 10/25/13 KAQUN has extra high stable dissolved Oxygen content Dissolved Oxygen reaches and enters cancer cells without hemoglobin contribution KAQUN triggers redox reactions in seconds as a proof of mitochondrial activity KAQUN triggers apoptosis as a proof of increased cell energy level and increased membrane potential KAQUN triggers cell redifferentiation as a proof of mitochondrial activity What do we know about KAQUN water?

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Prezentci cme 10/25/13 Thank you for your attention! 2013 EUROPEAN KAQUN SYSTEM ALL RIGHTS RESERVED info@kaqun.eu www.kaqun.eu Thank you for your attention! 2013 EUROPEAN KAQUN SYSTEM ALL RIGHTS RESERVED info@kaqun.eu