presentation of anti-obesity effects on unripe rubus coreanus miquel extract

8/10/2019 Presentation of Anti-Obesity Effects on Unripe Rubus Coreanus Miquel Extract

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Anti-Obesity Effects on Unripe

Rubus coreanus Miquel Extract inHigh Fat Diet-Induced Obese Mice

By:

ILMA INAROH AZIZAH

1101094


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BackgroundMortality and the

costs that are spent

to tackle the obesity-

related illnesses

Synthesizing

medicines, suchas orlistat, etc.

Using traditional

medicine such as

green tea extract is

better.

In Korea, Rubus

Coreanus can also

treat the obesity

((Jeong, MY. 2013).

The antiinflammatory

activity depend on the

degree of Rubus

coreanus ripening.

(Yang, HM. 2008)

This study will

distinguish the unripe

Rubus coreanus

Miquel as an anti-

obesity with the ripeone.


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Problems

What is the unripe Rubuscoreanus (uRC) activity on

obesity?

How does it compare to the ripe

Rubus coreanus (RC) ?


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Purpose

To determine the anti-obesity

effects of the unripe Rubus

coreanus (uRC) and compare it

to the ripe Rubus coreanus (RC)


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Literature Studies

Rubus Coreanus Miquel


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Obesity

According to WHO definition, the

Obesity is an overweight condition that isan abnormal or excessive fat

accumulation that may impair health.

Obesity is a condition in which excessbody fat has accumulated due to lipids

changing into adipocytes and an increase

in the number of differentiated mature

cells which are regulated by genetic and

environmental factors such as nutrients.

Adipocyte

tissue


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Rubus coreanus Miquel

Rubus Coreanus is a kind of berriesfound in South Korea, China and

Japan, commonly called Bokbunja

(Korea).

The dried fruit are used in traditional

herbal medicine for the treatment of

impotence, spermatorrhea,

enuresis,and asthma.


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Previous Works

Yang, HM.2008

Antiinflammatory Activities of Rubus coreanus

Depend on the Degree of Fruit Ripening.

Panchal,SK. 2012

Ellagic acid attenuates high-carbohydrate, high-

fat diet-induced metabolic syndrome in rats.

Jeong,

MY. 2013

Rubi Fructus (Rubus coreanus) InhibitsDifferentiation to Adipocytes in 3T3-L1 Cells.


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Material and Methods


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Results and Discussion


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IN VITRO

3T3-L1 cells

(preadipocytecells)

Cell CultureDifferentiatedinto adipocyte

cells

Added by extractof uRC and

extract of RC

Measured its cellsviability using

MTT assay

Measured LipidAccumulation

using Oil Red OStaining


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According to Cell Viabilitys graph

The lowest number of cell viability is on 300 concentration

either in RC or uRC, its cell viability was decreased about 15%of control cells.


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According to The Result using Oil Red

O-Stained


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The darker red color

of the cells, the greater

number of adipocytecells, which is

indicating that on the

uRC 300g/mLhas a

lower number of

adipocyte cells, so that

it has the anti-obesity

effects.


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And by using the graph of lipid accumulation vs concentration, has

indicated that the higher concentration of uRC, the lower lipid

accumulation. So that, these results indicate that uRC effectively blockadipocyte differentiation in 3T3-L1 preadipocytes.


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Results and Discussion


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IN VIVO

Normal

Diet(ND)

High Fat

Diet(HFD)

HFD+uRC

50mg/kg/day

HFD+uRC

100mg/kg/day

HFD+

Green Tea

Extract50 mg/kg/day

ND: 10% kcal from fat;

HFD: 60% kcal from fat;.


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In Vivo

Took the bloodand serum

samples from themice

Measured itsserum lipid

parameter usingLDX system

Analyze the result


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Result of Serum Lipid Parameter in using LDX

system


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Conclusion

uRC administration significantly suppressed lipidaccumulation, body weight gain, adiposetissue weight, and serum levels of TC, TG,

LDL-cholesterol, and glucose in the 3T3-L1adipocyte of high fat diet-induced obese

mice.

presentation of anti-obesity effects on unripe rubus coreanus miquel extract

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