8/7/2019 Presentation - Miniaturised Diagnostics for Health - Zulf Ali

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Miniaturised Diagnostics for Health

Zulfiqur Ali, (z.ali@tees.ac.uk)

School of Science and Technology

University of Teesside

Microdiagnostics, EKTN, NetPark, 1stJune 2009

8/7/2019 Presentation - Miniaturised Diagnostics for Health - Zulf Ali

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Miniaturised diagnostics for health

Increasing costs of healthcare

Demographics

Chronic diseases (heart disease, stroke,

cancer, diabetes, lung cancer) are leading

causes of mortality and increasing in

prevalence

Prevention and early detection of disease

Care at the point of need

More efficient approach for development

of drug therapies

Need for complex chemical and biologicaloperations at low cost in automated

manner to allow operation by non-

specialist staff

8/7/2019 Presentation - Miniaturised Diagnostics for Health - Zulf Ali

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Microfluidics

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Storage, dosing, moving and mixing smallvolumes (mL pL) of liquids in channelstypically 10-100Qm wide

Design and construction

Fabrication protocol

Fluidic control (pressure, electrokinetic,centrifugal, acoustic)

Detection (optical, electrochemical,acoustic)

Packaging, macro world interface (fluidic,mechanical, optical, electrical)

Materials - glass, silicon, polymer, hybrid

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DVT-IMP: aid diagnosis of deep vein

thrombosis DVT is the leading cause of unexpected death in

most developed nations

results from the formation of blood clots in deepveins, usually in the leg.

Diagnosis is problematic.

Requires blood test and clinical assessmentfollowed by medical imaging if required.

Current blood tests can only eliminate DVT.

Specificity of current tests results in false negativeresults, putting patients at risk.

http://www.diagnosingdvt.com

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DVT-IMP

Measure D-dimer concentration

in whole blood

Immunoassay using high

affinity antigen-antibody

binding

Impedance based detection

Microfluidics for sample

processing

Quantitative, accurate and

reproducible

Simple and easy to use Potential use in primary care

and clinics

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A

b -

.4 mg/ml

Ag -

pg/ml

Ag - 5

pg/ml

Ag - 4,5 ng/ml

Ag - 5

ng/ml

Ag - 4,5 g/ml

-

I

(Z)(;

2)

Re (Z) (;

2)

Nyquist Plots for varying model [ g]

Dynamic range: 100pg/ml 100ng/ml, Opt. temp. 20

C

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Transducer layer

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Diagnostic Cartridge

Micr idic body

dhesive foil

Electrode layer

Microfluidic body

dhesive foil

Electrode layer

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Roll-to-roll process

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