Pires & Dolan Mol. Biol. Evol. 2010

PIFs

HEC/IND

PREbHLH family

Carretero-Paulet et al. Plant Physiol. 2010

non DNA-binding bHLHs as regulators

Liljegren et al. Cell 2004

atypical bHLHs lack conserved glutamate residue in DBD

Gremski et al. Development 2007

HECATEs lack conserved glutamate

wild-type 35S:HEC2 35S:HEC3

Gremski et al. Development 2007

stigma/style defects in HEC mutants phenocopy spt

Gremski et al. Development 2007

HECs interact with SPT in Y2H

Carretero-Paulet et al. Plant Physiol. 2010

non DNA-binding bHLHs as regulators

Lee et al. Plant Cell Physiol. 2006

PRE proteins; PRE1 = PACLOBUTRAZOL RESISTANCE1

GA1 GA2

paclobutrazol

GA20 oxidasesGA3 oxidasesGA2 oxidases gibberellin pool

PRE overexpression triggers GA response

Lee et al. Plant Cell Physiol. 2006

GA1 GA2

paclobutrazol

GA20 oxidasesGA3 oxidasesGA2 oxidases gibberellin pool

PRE overexpression triggers GA response

Lee et al. Plant Cell Physiol. 2006

GA signaling constitutively

repressed

PRE overexpression triggers GA response

Lee et al. Plant Cell Physiol. 2006 Hyun et al. Plant Mol. Biol. 2006

PRE6/KIDARI overexpression affects light signaling

Hyun et al. Plant Mol. Biol. 2006

PRE6/KIDARI suppresses HFR1

Hyun et al. Plant Mol. Biol. 2006

Wang et al. Plant Cell 2009

PRE5/ATSB1 regulates BR response

PRE/BNQ may regulate DNA-binding bHLH activities

Mara et al. Plant Cell 2010

Loss of PRE/BNQ in flowers affects flower development

Mara et al. Plant Cell 2010

bnq3/pre4WT

Mara et al. Plant Cell 2010

PREs are regulated by floral ID genes (PRE=BNQ)

/PRE1/PRE2

/PRE4

Mara et al. Plant Cell 2010

Schlereth et al. Nature 2010

PRE3 is MP target (TMO) required for basal embryogenesis

Esmon et al. PNAS 2006

PRE2/BNQ2

SUBFAMILY XV: PRE GENES

AT5G39860

PRE1/BNQ1/ili1-D (PACLOBUTRAZOL-RESISTANCE1/BANQUO1/INCREASED LAMINA INCLINATION) bHLH136

Lee et al. Plant Cell Physiol 2006; Mara et al. Plant Cell 2010; Zhang et al. Plant Cell 2009

AT5G15160 PRE2/BNQ2 bHLH134Lee et al. Plant Cell Physiol 2006; Esmon et al. PNAS 2006

AT1G74500PRE3/TMO7/atbs1-D (TARGET OF MONOPTEROS7/AT-BRI1-SUPP) bHLH135

Lee et al. Plant Cell Physiol 2006; Schlereth et al. Nature 2010; Wang et al. Plant Cell 2009 MP/ARF5 target in embryos

AT3G47710 PRE4/BNQ3 bHLH161 Lee et al. Plant Cell Physiol 2006;

AT3G28857 PRE5 Lee et al. Plant Cell Physiol 2006;

AT1G26945 PRE6/KDR (KIDARI)Lee et al. Plant Cell Physiol 2006; Hyun & Lee Plant Mol Bio 2006

OTHER bHLHs

AT3G25710 TMO5 BHLH032 Schlereth et al. Nature 2010 MP/ARF5 target in embryos

SUBFAMILY VIIIb: HEC GENES

AT4G00120 EDA33/IND (INDEHISCENT) Liljegren et al. Cell 2004

AT5G67060 HEC1 (HECATE 1)

Gremski et al. Development 2006; Okushima et al. Plant Cell 2005 IAA-induction is ARF7-dependent

AT3G50330 HEC2 (HECATE 2) Gremski et al. Development 2006

AT5G09750 HEC3 (HECATE 3) Gremski et al. Development 2006

AT3G21330 bHLH family protein

RECENT bHLH REVIEWS/EVOLUTION

Pires & Dolan Mol. Biol. Evol. 2010

Carretero & Paulet Plant Phys. 2010 in press